KR20040018848A - pH CONTROL AND CONTAMINATION ALARM SYSTEM OF BIOREACTOR USED FOR PLANT TISSUE CULTURE - Google Patents

pH CONTROL AND CONTAMINATION ALARM SYSTEM OF BIOREACTOR USED FOR PLANT TISSUE CULTURE Download PDF

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KR20040018848A
KR20040018848A KR1020020050914A KR20020050914A KR20040018848A KR 20040018848 A KR20040018848 A KR 20040018848A KR 1020020050914 A KR1020020050914 A KR 1020020050914A KR 20020050914 A KR20020050914 A KR 20020050914A KR 20040018848 A KR20040018848 A KR 20040018848A
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concentration
value
bioreactor
measured
culture
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KR100496010B1 (en
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정석현
강창호
권기영
한길수
최홍기
이기명
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대한민국(관리부서:농촌진흥청)
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control

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Abstract

PURPOSE: A pH control and contamination alarm system of bioreactors for plant tissue culture is provided, thereby simultaneously controlling pH of several bioreactors and detecting contamination thereof at an early stage. Therefore, high quality products can be produced. CONSTITUTION: A pH control and contamination alarm system of bioreactors for plant tissue culture comprises a plurality of bioreactors(1,2,3); pH electrodes(4,5,6) installed within the bioreactors(1,2,3) and detecting pH of the medium in each bioreactor; an integrated controller(7) receiving pH values and processing them; a computer(13) connecting the integrated controller(7) and providing the processed data results; and a plurality of pumps(10,11,12) which introduce one solution selected from NaOH solution(9) and HCl solution(8) into the bioreactors(1,2,3) according to the pH measuring modules by the integrated controller.

Description

식물조직배양용 생물반응기 내 배양액 피이 에이치 농도 제어 및 오염경보장치{pH CONTROL AND CONTAMINATION ALARM SYSTEM OF BIOREACTOR USED FOR PLANT TISSUE CULTURE}PH Control and Contamination Alarm System for Bioreactors for Plant Tissue Culture {pH CONTROL AND CONTAMINATION ALARM SYSTEM OF BIOREACTOR USED FOR PLANT TISSUE CULTURE}

본 발명은 식물조직배양용 생물반응기내 배양액 pH 제어 및 오염경보장치를 위한 제어장치에 관한 것으로서, 더욱 상세하게는 나리류 등의 조직배양에 이용되는 복수의 생물반응기(bio-reactor) 내의 pH 농도를 배양에 적합한 농도로 제어하고 배양 중 배양액의 오염감염 여부를 경보하도록 하는 제어장치에 관한 것이다.The present invention relates to a control device for controlling the pH of the culture medium in the bioreactor for plant tissue culture and a pollution warning device, and more specifically, the pH concentration in a plurality of bio-reactor used for tissue culture such as lilies. The present invention relates to a control device for controlling the concentration to a concentration suitable for culturing and alarming whether or not the culture solution is contaminated.

나리류는 인편을 통한 영양번식을 하는 식물로서 구근은 전분 및 다른 여러 양분의 저장기관 역할을 하며 줄기가 생기는 생장점을 포함한다. 또한 구근은 바이러스 등에 의한 감염으로 수량이나 품질저하의 문제로 여러해 동안 지속적으로 사용할 수 없어 농가에서는 매년 새로운 구근을 구입하여 재배하고 있는 실정인 바 나리류의 종구를 성장속도가 빠르고 많은 양의 종구를 배양할 수 있도록 생물반응기(bio-reactor)를 이용한 조직배양방법 등을 통해 연구개발되어 왔다. 예를들어, 대한민국 특허등록번호 제198970호의 경우는 반응기 중간에 교반기(impeller)가 장착되고, 정제된 공기를 주입할 수 있도록 반응기 바닥에 많은 공기구멍이 형성되어 있는 생물반응기(bio-reactor)를 이용하여 산업적으로 대량생산하는 방법을 제시하고 있다.Lilium is a plant that propagates through human scales, and the bulb serves as a reservoir for starch and other nutrients, and includes a growth point for stems. In addition, bulbs can not be used continuously for many years due to infections caused by viruses, etc. due to problems in quantity and quality, so farmers buy new bulbs every year. It has been researched and developed through a tissue culture method using a bio-reactor to culture. For example, in the case of Korean Patent Registration No. 198970, a bio-reactor having an agitator in the middle of the reactor and having many air holes in the bottom of the reactor for injecting purified air is provided. It suggests how to mass produce industrially.

그러나 이러한 종래의 생물반응기(bio-reactor)를 이용한 나리 구근 등의 배양에는 2∼4 개월 정도가 소요되며, 또한 배양기간 중 주기적으로 배양액을 교체하며 배양하고 있어 배양에 많은 노력과 시간이 소요되는 문제점이 있다.However, it takes about 2 to 4 months to cultivate bulbs using the conventional bio-reactor, and also cultures by replacing the medium periodically during the incubation period. There is a problem.

본 발명은 상기한 문제점을 해결하기 위하여 창출된 것으로서 본 발명의 식물조직배양용 생물반응기내 배양액 pH 제어 및 오염경보장치는 복수의 생물반응기 (bio-reactor) 내의 pH 농도를 생육에 적합한 농도로 제어하여 배양기간의 단축을 도모하고, 또한 통합 컨트롤러(controller)를 이용하여 복수의 생물반응기(bio-reactor)를 동시에 관리하여 제어효율을 향상시키고 동시에 배양액의 오염감염 여부를 조기에 판단하여 효율적인 배양관리가 이루어질 수 있도록 하는 장치를 제공하려는 데 그 목적이 있다.The present invention was created in order to solve the above problems, the pH control and contamination alarm apparatus of the culture medium in the bioreactor for plant tissue culture of the present invention to control the pH concentration in the plurality of bio-reactor (bio-reactor) to a concentration suitable for growth To shorten the cultivation period, and to manage multiple bio-reactors at the same time by using an integrated controller to improve the control efficiency, and at the same time to determine whether the culture solution is contaminated early and effective culture management The purpose is to provide a device that can be made.

도 1은 본 발명에 따른 생물배양기내 배양액의 pH농도 제어 및 오염경보장치의 전체구성을 보여주는 구성도,1 is a block diagram showing the overall configuration of the pH concentration control and contamination alarm device of the culture medium in the bioculture according to the present invention,

도 2는 pH농도 제어과정을 간략히 나타내는 블럭도,2 is a block diagram briefly illustrating a pH concentration control process;

도 3은 pH농도 제어를 위한 pH측정비교모듈에 대한 제어 흐름도,3 is a control flowchart for a pH measurement comparison module for controlling the pH concentration,

도 4는 오염 감염 판단을 위한 pH변화량비교모듈에 대한 제어 흐름도이고4 is a control flowchart for a pH change comparison module for determining a contamination infection;

도 5는 본 발명의 작동과정을 보여주는 작동흐름도이다.5 is a flow chart showing the operation of the present invention.

<도면의 주요부분에 대한 부호의 설명><Description of Symbols for Main Parts of Drawings>

1,2,3생물반응기4,5,6pH 전극1,2,3 bioreactor 4,5,6 pH electrode

7통합컨트롤러8산(HCL) 용액7 Integrated Controller 8 Acid (HCL) Solution

9염기(NaOH) 용액10,11,12정량펌프9 NaOH solution 10, 11, 12 metering pump

13제어용 컴퓨터14경보장치13 Computer 14 alarm system for control

SA공기공급장치S30pH측정비교모듈SA air supply unit S30pH measurement comparison module

S220pH변화량비교모듈S220pH variation comparison module

상기한 목적을 달성하기 위하여, 본 발명의 식물조직배양용 생물반응기내 배양액 pH 제어 및 오염경보장치는 나리 구근 등의 조직배양에 이용되는 복수의 생물반응기(bio-reactor) 내 pH 농도를 동시에 제어하고, pH 농도의 변화로부터 배양액의 오염감염 여부를 판단하여 경보할 수 있도록 하는 것을 특징으로 한다.In order to achieve the above object, the culture medium pH control and contamination alarm device in the plant tissue culture bioreactor of the present invention simultaneously controls the pH concentration in a plurality of bio-reactor (bio-reactor) used for tissue culture such as bulbous bulbs And, it is characterized in that the alarm to determine whether the contamination of the culture solution from the change in pH concentration.

이하 본 발명에 따른 식물조직배양용 생물반응기내 배양액 pH 제어 및 오염경보장치의 구성 및 작용에 대한 바람직한 실시예를 첨부한 도면을 참조로 하여 보다 상세히 설명하면 다음과 같다.Hereinafter, with reference to the accompanying drawings, a preferred embodiment for the configuration and operation of the culture medium pH control and contamination alarm system in the plant tissue culture bioreactor according to the present invention will be described in detail.

첨부된 도면 중 도 1은 본 발명에 따른 생물배양기내 배양액의 pH농도 제어 및 오염경보장치의 전체구성을 보여주는 구성도이며 도 2는 pH농도 제어과정을 간략히 나타내는 블럭도이고 도 3은 pH농도 제어를 위한 pH측정비교모듈에 대한 제어 흐름도이며 도 4는 오염 감염 판단을 위한 pH변화량비교모듈에 대한 제어 흐름도이고 도 5는 본 발명의 작동과정을 보여주는 작동흐름도이다.In the accompanying drawings, Figure 1 is a block diagram showing the overall configuration of the pH concentration control and contamination alarm system of the culture medium in the bioculture according to the present invention, Figure 2 is a simplified block diagram showing the pH concentration control process and Figure 3 is a pH concentration control Figure 4 is a control flow chart for the pH measurement comparison module for Figure 4 is a control flow chart for the pH change comparison module for determining the contamination infection and Figure 5 is a flow chart showing the operation of the present invention.

하기에서 본 발명을 설명함에 있어, 관련된 공지 기능 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명은 생략할 것이며, 후술되는 용어들은 본 발명에서의 기능을 고려하여 정의된 용어들로서 이는 사용자, 운용자의 의도 또는 관례 등에 따라 달라질 수 있다. 그러므로 그 정의는 본 명세서 전반에 걸친 내용을 토대로 내려야 할 것이다.In the following description of the present invention, when it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted, and the following terms are used in the present invention. As defined in consideration of the terms, they may vary depending on the intention or custom of the user or operator. Therefore, the definition should be made based on the contents throughout the specification.

먼저 첨부된 도1은 복수의 생물반응기(bio-reactor) 내 배양액의 pH 농도를 제어하고, pH 농도의 변화로부터 오염감염 여부를 판단하여 경보하는 전체 시스템의 구성을 보여주는 구성도로서, 생물의 조직배양을 위한 복수의 생물반응기(bio-reactor)(1)(2)(3) 내의 pH 농도를 측정하기 위하여 상기 생물반응기의 상부 일정위치에 장착된 pH 전극(4)(5)(6)을 이용하여 생물반응기 내의 pH 농도를 계측하고 그 계측값을 통합 컨트롤러(7)로 전송하게 되며 상기 통합 컨트롤러(7)에서의 신호처리과정을 통해 미리 결정된 목표값과 비교한 후 적정량의 산(HCL, 0.1N)과 염기(NaOH, 0.1N) 용액(8)(9)을 상기 생물반응기(bio-reactor) 내로 주입하여 pH 농도를 제어하도록 하였다. 여기에서 산과 염기 용액의 공급에는 일정량을 공급하도록 하는 정량펌프 (10)(11)(12)를 이용하여 구동모터의 회전수 및 구동시간 등의 조절을 통해 적량의 산과 염기 용액이 상기 생물반응기(1)(2)(3) 내로 주입되도록 하였다. 또한 상기 pH 전극(4)(5)(6)을 통해 계측된 생물반응기 내의 pH 농도를 통합 컨트롤러(7)를 통해 받은 신호처리를 통해 제어용 컴퓨터(13)를 통해 미리 정해진 프로그램에 따라 분석하고 결과를 사용자로 하여금 볼 수 있도록 하며 만일 생물반응기 내가 오염되었을 경우에는 즉시 경보장치(14)가 작동되도록 구성되어져있다.First, Figure 1 is a schematic diagram showing the configuration of the entire system for controlling the pH concentration of the culture medium in the plurality of bio-reactor (bio-reactor), and determines and alerts to the presence of contamination from the change in pH concentration, the organization of the organism In order to measure the pH concentration in a plurality of bio-reactors (1) (2) (3) for cultivation, pH electrodes (4) (5) (6) mounted at a predetermined position on the top of the bioreactor are replaced. The pH concentration in the bioreactor is measured, and the measured value is transmitted to the integrated controller 7, and compared with a predetermined target value through signal processing in the integrated controller 7, and then an appropriate amount of acid (HCL, 0.1N) and a base (NaOH, 0.1N) solution (8) (9) were injected into the bio-reactor to control the pH concentration. Here, the acid and base solutions are supplied to the bioreactor by adjusting the rotational speed and the driving time of the driving motor using a quantitative pump 10, 11, 12 to supply a predetermined amount to the acid and base solution. 1) (2) (3). In addition, the pH concentration in the bioreactor measured by the pH electrodes 4, 5, 6 is analyzed according to a predetermined program through the control computer 13 through signal processing received through the integrated controller 7. It is configured to enable the user to see the alarm device 14 immediately if the bioreactor is contaminated.

상기에서 생물반응기(1)(2)(3) 하부 바닥에는 정제된 공기를 주입할 수 있도록 통기구가 형성되어져 있으며 상기 통기구를 통해 생물반응기 내로 정제된 공기를 공급하도록 하는 공기공급장치(SA)가 구성된다.The bottom of the bioreactor (1) (2) (3) is formed with a vent for injecting the purified air and the air supply device (SA) for supplying the purified air into the bioreactor through the vent It is composed.

제2도는 pH 농도를 제어하는 프로세스를 보여주는 블럭도로서, 상기 생물반응기(bio-reactor)(1)(2)(3) 내의 pH 농도를 pH측정비교모듈(S30)을 통해 측정된 pH 농도값을 제어목표값과 비교한 후 설정된 목표값으로 제어하도록 컴퓨터(13)와 함께 통합 컨트롤러(7)에서 정량펌프(10)(11)(12)를 구동시켜 산ㆍ염기용액(8)(9)을 상기 생물반응기 내로 투입시킴으로서 생물반응기(bio-reactor) 내 pH농도를 실시간으로 제어되는 것을 보여준다.2 is a block diagram showing a process for controlling pH concentration, wherein the pH concentration value measured by the pH measurement comparison module (S30) in the pH of the bio-reactor (1) (2) (3) And the acid / base solution (8) (9) by driving the metering pumps (10) (11) (12) in the integrated controller (7) together with the computer (13) to compare the control target value with the control target value. It is shown that the pH concentration in the bio-reactor is controlled in real time by introducing into the bioreactor.

제3도는 pH농도의 제어과정을 보인 pH 농도의 제어흐름도로서 도에서 알 수 있는 것처럼 pH 농도의 제어는 측정된 pH값과 목표설정값을 비교하여 산(HCL, 0.1N)과 염기(NaOH, 0.1N)를 생물반응기(bio-reactor) 내로 주입시켜 pH농도를 제어하도록 하는 것이다. 이 때 상기 산(HCL, 0.1N)과 염기(NaOH, 0.1N)의 공급에는 정량펌프를 이용하여 설정시간만큼 산 및 염기 용액이 생물반응기 내로 주입되도록 하며 pH 농도의 설정값은 Low 값과 High 값을 설정하여 그 범위 내에서 항상 유지되도록 하였다.3 is a control flow chart of pH concentration showing the process of controlling the pH concentration. As can be seen from the figure, the control of pH concentration compares the measured pH value with the target set value, and compares the acid (HCL, 0.1N) and base (NaOH, 0.1N) is injected into the bio-reactor to control the pH concentration. At this time, the acid (HCL, 0.1N) and the base (NaOH, 0.1N) is supplied to the acid and base solution is injected into the bioreactor for a set time using a metering pump and the pH concentration is set to Low and High The value is set so that it is always kept within the range.

이를 더욱 상세히 설명하면 상기 pH 농도의 제어흐름은 다음과 같은 단계로 이루어진다.To explain this in more detail the control flow of the pH concentration is made of the following steps.

생물반응기(1)(2)(3) 상에 장착설치된 pH 전극(4)(5)(6)을 통해 상기 생물반응기 내의 pH 농도를 계측하는 pH농도계측단계(S10);와PH concentration measurement step (S10) of measuring the pH concentration in the bioreactor through the pH electrode (4) (5) (6) mounted on the bioreactor (1) (2) (3); and

상기 pH농도계측단계(S10)에서 계측된 pH 값에 대하여 미리 설정된 Low 값과 High 값과 비교하는 설정범위비교단계(S20);와A setting range comparison step (S20) comparing the low value and the high value preset for the pH value measured in the pH concentration measuring step (S10); and

상기 설정범위비교단계(S20)에서는 계측된 pH 값이 미리 설정되어 입력된 Low 값과 High 값의 범위 내에 있을 경우, 이는 정상적인 운전상태를 나타내고 있으므로 귀환(feedback)(R1)되어 계속 pH를 계측하게 되며, 계측된 pH 값이 상기 설정범위 외에 있으면 다음 단계로 진행하게 된다.In the set range comparison step (S20), if the measured pH value is set in advance and falls within a range of the input low and high values, this indicates a normal operating state and is thus fed back (R1) to continuously measure the pH. If the measured pH value is outside the set range, the process proceeds to the next step.

상기 설정범위비교단계(S20)에서 계측된 pH 값과 Low 설정값을 비교하는 pH측정비교모듈단계(S30);와PH measurement comparison module step (S30) for comparing the pH value and the low set value measured in the set range comparison step (S20); and

상기 pH측정비교모듈단계(S30)에서는 계측된 pH 값이 설정된 Low값 이하일 경우에는 염기(NaOH)용액(8)을 공급하도록 정량펌프(10)(11)(12)를 구동하여 염기를 보충하도록 하며, 계측된 pH 값이 설정된 High값 이상일 경우에는 산(HCL)용액(9)을 공급하도록 정량펌프(10)(11)(12)를 구동시켜 산을 보충하도록 함으로써 상기 생물반응기(bio-reactor)내의 pH 농도가 설정범위 내에서 항상 일정한 값을 유지하도록 한다.In the pH measurement comparison module step (S30), when the measured pH value is lower than or equal to the set Low value, the quantitative pumps (10) (11) (12) are driven to supply the base (NaOH) solution (8) to replenish the base. When the measured pH value is equal to or higher than the set High value, the bio-reactor is operated by supplementing the acid by driving the metering pumps 10, 11, and 12 to supply the acid (HCL) solution 9. Keep the pH concentration within) constant at all times within the set range.

상기 pH측정비교모듈단계(S30)에서의 산과 염기의 공급을 통해 생물반응기 내의 pH 값은 조절되어질 것이며 이러한 조절된 pH농도 값을 설정범위 내에 있는 가를 비교하는 조절pH농도값비교단계(S40);로 이루어진다.PH value in the bioreactor will be adjusted through the supply of acid and base in the pH measurement comparison module step (S30) and comparing the adjusted pH concentration value (S40) comparing the adjusted pH concentration value within the set range; Is made of.

상기 조절pH농도값비교단계(S40)에서는 상기 pH측정비교모듈단계(S30)에서의 산과 염기용액의 공급을 통해 생물반응기 내에서 조절되어지는 pH 값을 지속적으로pH 전극(4)(5)(6)으로 하여금 측정함으로서 측정된 pH농도의 계측값이 설정범위 내에 있게되면 상기 pH농도계측단계(S10)로 귀환(feedback)(R2)하게 되어 생물반응기 내의 pH 농도를 계속 측정하게 되고, 측정된 pH 계측값이 설정범위 내에 있지 않으면 상기 pH측정비교모듈단계(S30)로 귀환(R3)되도록 하여 pH 농도를 지속 조절하도록 한다.In the control pH concentration comparison step (S40), the pH value that is controlled in the bioreactor is continuously maintained by supplying an acid and a base solution in the pH measurement comparison module step (S30). 6) When the measured value of the measured pH concentration is within the set range by measuring, it returns to the pH concentration measuring step (S10) (R2) to continue to measure the pH concentration in the bioreactor, If the pH measurement value is not within the setting range, the pH measurement comparison module step (S30) is returned (R3) to continuously adjust the pH concentration.

제4도는 생물반응기(bio-reactor) 내 배양액의 오염에의 감염 여부를 판단하는 흐름도이다. 도에서 알 수 잇는 것처럼 배양액의 오염에의 감염 여부를 판단하는 과정은 다음과 같이4 is a flow chart for determining whether the culture medium in the bio-reactor is infected with contamination. As can be seen from the figure, the process of determining whether the culture medium is infected with contamination is as follows.

생물반응기 상에 장착설치된 pH 전극(4)(5)(6)에 의하여 생물반응기 내의 pH 농도를 측정하는 pH 농도측정단계(S200);와PH concentration measurement step (S200) of measuring the pH concentration in the bioreactor by the pH electrode (4) (5) (6) mounted on the bioreactor; and

상기 pH 농도측정단계(S200)에서 측정된 pH 농도값을 미리 설정된 시간 간격으로 저장하도록 하는 pH측정값저장단계(S210);와PH measurement value storage step (S210) to store the pH concentration value measured in the pH concentration measurement step (S200) at a predetermined time interval; and

상기 pH측정값저장단계(S210)를 통해 저장된 각 pH농도 측정값(X1, X2, X3...Xn-1,Xn)들에 대하여 각 측정값의 변화량(ΔX = Xn- Xn-1)을 계산하고 그 변화량을 미리 설정된 업셋(offest) 설정값과 비교함으로서 다음 단계를 수행토록 하는 pH변화량비교모듈단계(S220);로 이루어져 있음을 알 수 있다.Change amount (ΔX = X n ) of each measured value for each pH concentration measured value (X 1 , X 2 , X 3 ... X n-1 , X n ) stored through the pH measured value storing step (S210) It can be seen that the pH change module comparison step (S220) to perform the next step by calculating the X n-1 ) and compare the change amount with the preset upset (offest) set value.

상기 pH변화량비교모듈단계(S220)에서는 시간별 전후 측정값을 상호 차감비교하여 그 차감값인 pH농도측정값변화량(ΔX = Xn- Xn-1)을 구하고 그 측정값변화량을 미리 설정된 업셋(offset)설정값과 비교함으로서 다음단계를 수행토록 하는 바,상기 측정값변화량이 업셋(offset)설정값 이상일 경우 즉, 급격한 pH 농도의 저하를 나타낼 경우에는 오염 발생으로 판단하도록 하는 오염 상태를 나타내므로 즉각적으로 오염경보가 작동(S230)되도록 하며, 상기 측정값변화량이 설정값 이하일 경우에는 비오염상태를 나타내므로 상기 pH변화량비교모듈단계(S220)에로 다시 귀환(feedback)(R4)시켜 반복 수행하도록 한다.In the step of comparing the pH change module step (S220), the measured value change amount (ΔX = X n -X n-1 ), which is the subtraction value, is obtained by comparing the difference between before and after measured values by time, and the preset upset ( By comparing the set value with the set value), the next step is performed. When the measured value change amount is more than the offset set value, that is, when the abrupt pH concentration is lowered, the contamination state is determined. Pollution alarm is immediately activated (S230), and if the measured value change amount is less than the set value indicates a non-pollution state, so as to return to the pH change amount comparison module step (S220) (R4) to perform repeatedly do.

이상과 같이 구성된 본 발명의 식물조직배양용 생물반응기내 배양액 pH 제어 및 오염경보장치에 대한 작동 상태를 도5를 통하여 설명하면,Referring to Figure 5 illustrates the operating state of the culture medium pH control and contamination alarm system in the plant tissue culture bioreactor configured as described above,

공기공급장치(SA)에 의하여 정제된 공기가 생물반응기(1)(2)(3) 내로 투입되고 상기 생물반응기에 장착설치된 pH 전극(4)(5)(6)에 의하여 생물반응기 내의 pH 농도를 계측하게 되며 상기 계측된 pH 농도값은 모든 데이터를 통합처리하는 통합컨트롤러(7)로 보내져 입력되며 상기 입력된 계측값에 따른 정보는 컴퓨터(PC)(13)를 통하여 사용자로 하여금 알 수 있도록 한다. 상기 통합컨트롤러(7)에 입력된 pH측정값과 설정범위(High, Low)에 대한 pH측정비교모듈(S30)를 통하여 pH값이 Low 설정값 이하이면 정량펌프(10)(11)(12)를 작동시켜 염기(NaOH)용액(8)을 상기 생물반응기 내로 투입시키고, pH값이 High 설정값 이상이면 정량펌프(10)(11)(12)를 작동시켜 산(HCL)용액(9)을 상기 생물반응기 내로 투입시켜 pH 농도를 조절하도록 한다. 또한 상기 통합컨트롤러(7)에의 설정된 시간별로 입력된 pH 측정값에 대한 전후값의 차이량를 산정한 pH농도 변화량(ΔX)을 미리 설정한 업셋설정값과 비교하도록 하는 pH변화량비교모듈(S220)을 통하여 상기 pH농도 변화량(ΔX)이 상기 업셋설정값 이하인 경우에는 귀환토록 하여 반복수행시키도록 하고 상기 pH농도 변화량(ΔX)이 상기 업셋설정값 이상인 경우에는 경보기(14)를 통해 즉각 오염경보작동 (S230) 하도록 한다.The air purified by the air supply (SA) is introduced into the bioreactor (1) (2) (3) and the pH concentration in the bioreactor by the pH electrodes (4) (5) (6) installed in the bioreactor. The measured pH concentration value is sent to the integrated controller 7 which integrates all data and inputs the information according to the input measured value so that the user can know it through the computer 13. do. If the pH value is less than the low set value through the pH measurement comparison module (S30) for the pH measurement value and the set range (High, Low) input to the integrated controller (7), the metering pump (10) (11) (12) Base (NaOH) solution (8) is introduced into the bioreactor, and when the pH value is higher than the high set value, the metering pumps (10) (11) (12) are operated to operate the acid (HCL) solution (9). Into the bioreactor to adjust the pH concentration. In addition, the pH change amount comparison module (S220) for comparing the pH concentration change amount ΔX, which calculates the difference between the before and after values for the pH measured values input to the integrated controller 7 with the preset upset setting value, If the pH concentration change value ΔX is less than or equal to the upset set value, the control unit may return to repeat the operation. If the pH concentration change value ΔX is greater than or equal to the upset set value, an immediate contamination alarm operation may be performed through the alarm 14 S230).

상기와 같은 구성을 갖춘 시스템을 통하여 생물반응기 내의 pH 농도를 항상 일정한 값이 유지되도록 함으로서 효율적인 배양이 가능하게 된다.Through the system having the above configuration, the pH concentration in the bioreactor is always maintained at a constant value, thereby enabling efficient culture.

이상과 같이 본 발명에 의하면, 통합 컨트롤러를 이용하여 나리류 등의 조직배양에 이용되는 복수의 생물반응기(bio-reactor) 내 pH 농도를 동시에 제어함으로서 제어효율의 향상과 고품질의 배양물 생산을 도모하고 오염감염 여부를 조기에 판단함으로서 효율적인 배양관리가 이루어질 수 있는 효과를 갖는다.As described above, according to the present invention, by simultaneously controlling the pH concentration in a plurality of bio-reactor used for tissue culture such as Lilium using an integrated controller to improve the control efficiency and to produce high-quality cultures And by judging whether the infection is contaminated early has the effect of efficient culture management.

Claims (3)

복수의 식물조직배양용 생물반응기(bio-reactor);와A plurality of plant tissue culture bio-reactors; and 상기 각 생물반응기에 장착설치되어 각 생물반응기 내 배양물의 pH 농도를 계측하는 pH 전극;과A pH electrode installed in each bioreactor to measure the pH concentration of the culture in each bioreactor; and 상기 pH 전극에 의해 계측된 각각의 pH 농도측정값을 입력받아 통합처리하는 통합컨트롤러;와An integrated controller which receives and integrates each pH concentration measurement value measured by the pH electrode; and 상기 통합컨트롤러와 연결되어 각종 정보처리결과를 제공하는 컴퓨터;및A computer connected to the integrated controller to provide various information processing results; and 상기 통합컨트롤러에 의한 pH측정모듈에 따라 염기(NaOH)용액 및 산(HCL)용액(9) 중 하나를 선택하여 상기 생물반응기 내로 투입시켜 pH 농도를 조절하도록 하는 복수의 정량펌프;A plurality of metering pumps configured to select one of a base (NaOH) solution and an acid (HCL) solution (9) according to the pH measurement module by the integrated controller and to adjust the pH concentration by introducing into the bioreactor; 로 이루어져 복수의 생물반응기 내 배양물의 pH농도를 항상 일정한 값을 유지하도록 구성되는 것을 특징으로 하는 식물조직배양용 생물반응기 내 배양물의 pH농도 제어장치.The pH concentration control device of the culture in the bioreactor for plant tissue culture, characterized in that consisting of a plurality of bioreactor to maintain a constant pH concentration of the culture in the culture. 상기 제1항의 제어장치에서 상기 통합컨트롤러에 입력된 pH농도 측정값에 대한 시간별 전후입력값의 차이량를 산정한 pH농도 변화량(ΔX)을 미리 설정한 업셋설정값과 비교하도록 하는 pH변화량비교모듈을 통하여 상기 pH농도 변화량이 상기 업셋설정값 이상 시 오염경보가 작동하도록 하는 것이 부가되는 것을 특징으로 하는 복수의 식물조직배양용 생물반응기 내 배양액의 pH 농도 변화량을 분석하여 조기에 오염발생여부를 판단하고 경보하는 식물조직배양용 생물반응기 내 배양물의 오염경보장치.The pH change amount comparison module for comparing the pH concentration change amount (ΔX) calculated by calculating the difference between the time value before and after the pH concentration measurement value input to the integrated controller in the controller of claim 1 with the preset upset set value; Through the analysis of the pH concentration change of the culture medium in a plurality of plant tissue culture bioreactor, characterized in that it is added to enable the operation of the pollution alarm when the pH concentration change is above the upset set value to determine whether the contamination occurred early Pollution alarm device of culture in bioreactor for plant tissue culture. 청구항 제1항 또는 2항 중 어느 한 항에 있어서,The method according to claim 1 or 2, 상기 pH측정모듈은The pH measuring module 생물반응기(1)(2)(3) 상에 장착설치된 pH 전극(4)(5)(6)을 통해 상기 생물반응기 내의 pH 농도를 계측하는 pH농도계측단계(S10);와PH concentration measurement step (S10) of measuring the pH concentration in the bioreactor through the pH electrode (4) (5) (6) mounted on the bioreactor (1) (2) (3); and 상기 pH농도계측단계(S10)에서 계측된 pH 값에 대하여 미리 설정된 Low 값과 High 값과 비교하는 설정범위비교단계(S20);와A setting range comparison step (S20) comparing the low value and the high value preset for the pH value measured in the pH concentration measuring step (S10); and 상기 설정범위비교단계(S20)에서 계측된 pH 값과 Low 설정값을 비교하여 계측된 pH 값이 설정된 Low값 이하일 경우에는 염기(NaOH)용액(8)을 공급하도록 정량펌프(10)(11)(12)를 구동하여 염기를 보충하도록 하며, 계측된 pH 값이 설정된 High값 이상일 경우에는 산(HCL)용액(9)을 공급하도록 정량펌프(10)(11)(12)를 구동시켜 산을 보충하도록 함으로써 상기 생물반응기(bio-reactor)내의 pH 농도가 설정범위 내에서 항상 일정한 값을 유지하도록 하는 pH측정비교모듈단계(S30);와When the pH value measured in the set range comparison step (S20) and the measured pH value is less than the set Low value, the metering pump (10, 11) to supply a base (NaOH) solution (8) (12) to replenish the base, and when the measured pH value is higher than the set High value, the metering pumps (10) (11) (12) are driven to supply the acid (HCL) solution (9). PH measurement comparison module step (S30) to ensure that the pH concentration in the bio-reactor to always maintain a constant value within the set range by replenishing; and 상기 pH측정비교모듈단계(S30)에서의 산과 염기용액의 공급을 통해 생물반응기 내에서 조절되어지는 pH 값을 지속적으로 pH 전극(4)(5)(6)으로 하여금 측정함으로서 측정된 pH농도의 계측값과 설정범위를 비교하여 설정범위 내에 있게되면 상기 pH농도계측단계(S10)로 귀환(feedback)(R2)하게 되어 생물반응기 내의 pH 농도를 계속 측정하게 되고, 측정된 pH 계측값이 설정범위 내에 있지 않으면 상기 pH측정비교모듈단계(S30)로 귀환(R3)되도록 하여 pH 농도를 지속 조절하도록 하는 조절pH농도값비교단계(S40);The pH concentration measured by continuously measuring the pH value which is adjusted in the bioreactor by supplying the acid and base solution in the pH measurement comparison module step (S30). When the measured value and the set range is within the set range, it is fed back to the pH concentration measuring step (S10) (R2) to continuously measure the pH concentration in the bioreactor, and the measured pH measured value is the set range. If it is not within the pH measurement comparison module step (S30) to return (R3) adjusted pH concentration value comparison step to continuously adjust the pH concentration (S40); 로 이루어지는 것을 특징으로 하는 식물조직배양용 생물반응기 내 배양물의 pH농도 제어 및 오염경보장치.PH concentration control and contamination alarm device of the culture in the bioreactor for plant tissue culture, characterized in that consisting of.
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