KR20040016585A - Provender sterilization composition matter - Google Patents

Abstract

PURPOSE: Provided is a composition for in vivo detoxification of the product of lipid oxidation and in vivo sterilization of decomposition bacteria, thereby reducing disease occurrence and the use of antibiotics, and increasing productivity. CONSTITUTION: A composition for in vivo detoxification of product of lipid oxidation and in vivo sterilization of decomposition bacteria comprises 10 wt.% of water soluble ethoxyquin, 5 wt.% of biphenyl-dimethyl-dicarboxylate, 5 wt.% of Schizandra spp. extract, 5 wt.% of Artemisia capillaris THUNB. extract, 5 wt.% of ginkgo leaf extract, 5 wt.% of Melilotus extract, 5 wt.% of Saururus Chinesis(Lour) Bail extract, 5 wt.% of Houttuynia Cordata extract, 5 wt.% of Agrimonia pilosa LEDEB. extract and 50 wt.% of diluent. It is added to feed stuffs for livestock or fishes.

Description

Composition for the detoxification of fatty acid products in feed and the sterilization of decaying bacteria in the body {Provender sterilization composition matter}

The present invention relates to a composition for the body detoxification of the fatty acid products contained in the feed and the sterilization of the decaying bacteria in the body, more specifically, the disease in the growth period by continuously ingesting the fat oxide and perishable bacteria contained in the feed animal The present invention relates to a composition for the detoxification of fatty acid products contained in feed and the sterilization of perishable bacteria in the body, which has dramatically improved the symptoms of development and growth.

In general, feedstocks are cultivated, harvested, stored and distributed on a large scale, or because they use food by-products, so they are not as precise as quality control. Therefore, the fat contained in the feedstock is partially oxidized, so that various forms of decaying bacteria are growing in the distribution process.

Conventionally, when the fat contained in the feedstock has already been oxidized to a certain level, the fat oxide cannot be inactivated with a powder-type antioxidant. Animals ingesting the feed prepared by combining such raw materials are endogenous defense factors in the body. After decayed, peroxidation of tissue cells proceeded, and after prolonged development, various cell degenerations, including inflammation, occurred.

The primary changes are the peroxidation of liver tissue cells, which leads to local liver damage and the hematologically elevated levels of oxygen free radicals resulting in hemolysis of erythrocytes and peripheral blood vessels. Thrombosis resulted in blood circulation disorders.

High levels of Oxygen free radicals in the blood and peripheral blood circulation impede the destruction of other tissue cells in the body, causing growth delays and frequent digestive and respiratory diseases. Fatty acid levels of corn and fishmeal used as feedstocks are shown in Table 1 below.

This is an average value of the harvest of corn and fish meal used in feed companies for one year. The measurement technique conformed to the standard technique specified in the American Oil Chemistry Society (AOCS), and a solution of chloroform: methanol: distilled water in a weight ratio of 2: 1: 1 was used as an organic solvent for complete extraction of fat. UV-Visible Spectrophotometer was used.

TABLE 1

division POV (Peroxide Value) AnV Total-Aflatoxin Corn (from China) 70.01 38.31 15 ppb Corn (Argentina) 75.57 13.00 18 ppb Corn (US) 64.86 18.02 8 ppb Corn (Brazil) 51.25 16.78 18 ppb Dairy cattle feed 43.8 31.13 17 ppb Dog food (domestic) 52 8.4 14 ppb Dog food (foreign) 83.14 27.67 20 ppb Eel Feed 83.57 47.87 Salmonella, Streptococcus, Vibrio Flatfish Feed 74.17 31.14 Salmonella, Streptococcus, Vibrio Shrimp Feed 88.07 63.24 Salmonella, Streptococcus

Most feed ingredients and commercial feeds in Korea contain a large amount of fatty oxides, and perishable bacteria are detected at a significant density. This level is significantly higher than the POV (peroxide 5 or less) of stable foods, which causes toxicity in animals.

In this way, the fat oxides contained in feedstocks cause hepatic peroxidation to damage liver tissue cells, and the rise of free radicals in the blood causes major organs and cell membrane peroxidation of organs, causing damage. Meanwhile, free radicals in the blood cause peripheral blood vessel disorders by generating blood clots by hemolysis of red blood cells in peripheral blood vessels.

Adipose metabolism and peripheral vascular dysfunction due to peroxidation of liver tissue cells promote tissue damage due to a decrease in the supply of metabolic nutrients to the organs of the peripheral organs and organs.

Decaying bacteria are attached to the gastrointestinal mucosa and secrete toxins, damaging the gastrointestinal mucosa, disrupting the intestinal flora and causing diarrhea. Perishable bacteria infected in the body with damaged gastrointestinal mucosa cause disease by infecting major organs damaged by free radicals in the blood, directly damaging the major organs, and providing secondary pathogenic bacterial infection pathways.

Thus, peroxidation of tissue cells is induced by free radicals and peripheral vascular hemostasis in blood, and damage to tissue cells is deteriorated by toxins of perishable bacteria, which can lead to disease if prolonged. The above symptoms occur temporarily in a short time, but most of them develop into a disease form as they enter the later stages of growth.

Conventional attempts have been made to prevent vitamin E and stable yeast selenium by overdose. However, due to the excessive amount of exogenous lipid peroxide, it was difficult to alleviate the symptoms, and added glutathione, SOD enzyme, etc. It was things to do.

Vitamin E is well known for eliminating free radicals, which are produced during nutrient metabolism in the body of animals. This is a function of protecting tissue cells from free radicals produced by metabolic processes in the body in the absence of toxicity because the fat oxide intake is minimal in the case of a diet with a low oxidation level of food such as food.

However, since the amount of fat oxide in food is higher than that of food, vitamin E is mostly destroyed before it is transferred to tissue cells. Therefore, antioxidant activity was not effective in the body.

Selenium, glutathione, and SOD enzymes are well known as endogenous defense factors in vivo, but they have been used to protect tissue cells from free radicals generated during metabolism in vivo when consuming fresh, unoxidized fat.

Since the level of fat oxide is several times higher than that of food, the endogenous defense factor of liver tissue cells is destroyed from the ingested fat oxide and peroxidation of liver tissue cell membrane leads to abnormal free radical levels. Will rise. Because selenium has its own toxicity, it is well known that glutathione peroxidase synthesis does not increase significantly organically because overdosage and ingested fat oxides damage the liver tissue cell membranes, thereby degrading function.

Vitamin E, glutathione, and SOD enzymes can be temporarily administered to protect liver tissue cell membranes. However, due to the high cost of feed production, temporary treatment is possible, but continuous detoxification from sustained intake of fatty oxides is not possible.

Therefore, there is a need to find a means to inactivate the fat oxide contained in the feed in the animal body.

In addition, feedstock with a high fatty acid level had the following problems.

1) The content of fatty oxide is high.

2) As the fat is oxidized, the viscosity of the fat decreases and the perishable bacteria multiply accordingly. It contains a small amount of cytotoxin, inflammatory toxin and neurotoxin secreted by the decaying bacteria. Livestock, farmed fish, which continuously consume feed prepared by combining feed ingredients containing excessive or extremely low levels of toxicity, have the following problems in the body.

1) Hepatic tissue cytoplasm is deposited by the cellulose (Peroxide linkage protein, oxidized pigments) is reduced liver function.

2) The level of free radicals in the blood is elevated due to the peroxidation of liver tissue cells, which causes peroxidation of major organ cells in the body, thereby degrading the function of the major organs and causing the bacterial infection.

3) Because of the high level of free radicals in blood, hemolysis of peripheral blood vessels causes thrombosis.

4) In the state where the gastrointestinal mucosa is partially damaged by high levels of free radicals in the blood, decaying bacteria attach to the gastrointestinal mucosa to secrete toxins, which in turn damages the gastrointestinal mucosa, and destroys the intestinal flora, digestive disorders and diarrhea. Occurs.

5) Perishable bacteria are infected into the body through partially damaged parts of the gastrointestinal tract mucosa, and may induce inflammation or worsen damage in major organs and organs in the body, thereby providing a path for secondary bacterial infection.

Therefore, there is a need for an effective feed preparation method that neutralizes the fat oxide contained in the feed until it reaches the hepatocytes, eliminates free radicals in the blood, improves peripheral blood circulation disorders, sterilizes rot-causing bacteria and eliminates inflammation in the body. .

The present invention was developed in view of the conventional problems, and an object of the present invention is to protect the liver cells by inactivating the fat oxide contained in the feed from the gastrointestinal tract to the liver tissue cells, and to reduce the level of free radicals in the blood. It inhibits the peroxidation reaction of major organs and organs in the body, improves blood circulation disorders of peripheral blood vessels, and increases metastasis of metabolic nutrients and endogenous defense factors to promote recovery of peripheral tissues damaged by free radicals. , Detoxification of decaying bacteria in the intestine, decay of infected bacteria infected in the body, and anti-inflammation of fatty acid products contained in feeds that suppress the spread of disease through the elimination of inflammation formed in the body In providing a method.

The present invention adds water-soluble ethoxyquine to the feed to inactivate fatty oxides in the body, and adds biphenyl-dimethyl-dicarboxylate (BDD) and Schisandra chinensis extract to the blood free radicals in animals. Scavenging, ginkgo biloba leaf extract, electric leaf extract (Melilotus extract) is added to improve peripheral blood circulation disorders, and jinjin mugwort extract is added to promote liver detoxification function by detoxifying toxins secreted by decaying bacteria, triticale extract, By adding Echochochocho, Seonchochochocho extract, it is possible to simultaneously perform intestinal sterilization of perishable bacteria, sterilization of infected perishable bacteria, and elimination of inflammation.

Inactivating fatty acid of water-soluble ethoxyquin in the body is as follows.

As the antioxidant for feed, the fatty acid products that reached the liver cells by adding water-soluble ethoxyquine, which is emulsified or encapsulated fat-soluble ethoxyquine to improve absorption in the intestine, and inactivated fatty oxide from the gastrointestinal tract, Ethoxyquin inactivates the fatty oxide prior to reaction.

In this way, the fatty oxides are effectively removed from the body, while hydrogen is supplied to the seroids deposited in the hepatocytes to inactivate lipid peroxide and to decompose and inactivate the seroids.

The action of scavenging free radicals in blood of non-pentyl dimethyl dicardoxylate (hereinafter referred to as BDD) or Schisandra chinensis extract is as follows.

BDD and Schisandra chinensis extracts are well known as antioxidants in vivo, and among the components contained in Schisandra chinensis extract, the antioxidant substance is BDD, and recently, it is chemically synthesized and marketed under the name BDD. However, BDD and Schisandra chinensis extract are added at the same time because the level of free radicals in the blood of farmed fish and livestock animals is too high.

Ethoxyquine inhibits the oxidation of BDD and Schisandra chinensis extracts with fatty oxides, since it inactivates the fatty oxides before they react with the antioxidants of BDD and Schisandra chinensis extracts. Therefore, BDD and Schisandra chinensis extract are introduced into blood vessels without loss of titer via liver tissue, effectively eliminating free radicals in blood.

Ginkgo biloba leaf extracts and cultivated leaf extracts (Melilotus extracts) improve the vascular dysfunction and help BDD and Schizandra extracts to effectively eliminate free radicals in peripheral blood vessels. Such complementary actions can only be effective.

Effective free radical scavenging action of BDD and Schisandra chinensis extract inhibits the peroxidation of peripheral tissue cells.

Ethoxyquine is a powerful antioxidant that prevents the loss of potency due to oxidation when added to fat-soluble vitamins and glutathione, including vitamin E. Therefore, it can prevent the potency loss of BDD and Schisandra chinensis extract.

Since most fatty oxides are inactivated in the body by the action of ethoxyquine, BDD and Schisandra chinensis extract are transferred to the blood via liver tissue. Ginkgo biloba leaf extracts and melilotus extracts improve blood circulation impairment in peripheral blood vessels, allowing antioxidants of BDD and Schisandra chinensis extract to effectively eliminate free radicals in peripheral blood vessels. In addition, by suppressing free radicals in the blood, the level of free radicals in the blood is suppressed, thereby inhibiting cellular peroxidation of major organs and organs in the body.

Ginkgo biloba leaf extracts and M. foliaceae extracts (Melilotus extracts) are added to improve peripheral blood circulation disorders as follows.

Peripheral vascular disorders are resolved by adding ginkgo biloba extract or erythropoie extract (Melilotus extract) in order to dissolve blood clots and expand blood vessels caused by elevated free radicals.

By eliminating peripheral blood circulation disorders, peripheral tissue metastases of metabolic nutrients supplied by improved liver function may be increased and blood free radicals may be added to restore damaged peripheral tissues.

Improvement of liver function is achieved by water-soluble ethoxyquine and phosphorus mugwort extract. Soluble ethoxyquine neutralizes fatty oxides in liver tissue and phosphorus fungus extract promotes the function of detoxifying toxins secreted by rot bacteria in the liver. Remove it.

When the liver function is improved by this toxin elimination effect, the metabolized nutrients in the liver are increased, which increases the supply of nutrients to peripheral tissues by improving the peripheral blood circulation of Ginkgo biloba leaf extract and Mellilotus extract. do. Therefore, by complementary action like this must be mixed and added to exert the function.

The effect of detoxifying the toxins secreted by rot bacteria by promoting the liver detoxification function by adding Injin mugwort extract is as follows.

The decaying bacteria in the feed secrete bacterial toxins during infection, or infect the animal's body to secrete cellular toxins, inflammatory toxins, neurotoxins, etc. As a result, major organs and organs can be protected from toxins.

Intestinal sterilization of decaying bacteria and the disinfection of infected decayed bacteria and the elimination of inflammation with the addition of three hundred sec extract, fish extract and Seonchocho extract are as follows.

Fish paste and Seonhakcho extract are added to feed to prevent the attachment of decaying bacteria to the gastrointestinal mucosa. Sterilized bacteria that are already infected are sterilized while anti-inflammatory action of trichophytium extract occurs locally on major organs and organs. Relieves inflammation

Perishable bacteria that are continuously infected through feed in the intestine, and bacteria that infect the body to inflame and remove the inflammation, water soluble ethoxyquine, BDD, Schisandra chinensis extract, Ginkgo biloba extract, electric leaf extract (Melilotus extract), Injin mugwort extract can restore damaged liver and peripheral tissues.

This antimicrobial and anti-inflammatory action prevents tissue damage caused by decaying bacteria, neutralizes fatty acid products, decreases free radicals in blood, improves peripheral blood circulation disorders, and hepatic toxin detoxification. Keep your health at its best

Hereinafter, the preferred embodiment of the present invention will be described in detail.

Example 1

In order to conduct the experiment on the chicken of the present invention, the 39-day-old laying hens were divided into a control group, an experimental group 1, and an experimental group 2 to relieve stress for 7 days, and then administered the following experimental feed for 4 weeks.

Control group: Experimental feed administration

Experimental group 1: 0.015% by weight of water-soluble ethoxyquine was added to 100% by weight of the experimental feed.

Experimental Group 2: 0.015% by weight of water-soluble ethoxyquinone, 0.005% by weight of Schisandra chinensis extract (98Brix), 0.002% by weight of Ginkgo leaf extract (98Brix), 0.005% by weight of electric leaf extract (Melilotus extract) (98Brix) , 0.003% by weight of jinjin mugwort extract (98Brix), 0.01% by weight of tritical herb extract (98Brix), 0.01% by weight of fish herb extract (98Brix), 0.01% by weight of Seoncho herb extract (98Brix), and the extract was adsorbed on adsorbent silica Mixed with laying hen diet.

Experimental group 1 was administered only water-soluble ethoxyquine to compare the efficacy of the other additives. To verify the effect, blood biochemical analysis and egg quality judgment were performed at the end of the experiment.

Equipment used for blood biochemical analysis: CH-100 PLUS from SEAC, Italy, used as a reagent of Stanbio company, and the standard serum was calibrated for each analysis item and examined.

Blood biochemical analysis of the control group is as follows.

GOT 224-314U / L, GPT 26-32U / L, GGT 16-21U / L,

Total Cholesterol 109-95 mg / dL,

Triglycerides 53-67mg / dL, Phospholipid 169.7-204.1mg / dL,

Total-protein 3.2 ~ 3.4g / dL, Albumin 1.4 ~ 1.8g / dL,

Globulin 1.8-1.6 g / dL,

Blood biochemical analysis of experimental group 1

GOT 176-188U / L, GPT 6-7U / L, GGT 14-18U / L,

Total Cholesterol 179-181 mg / dL,

Triglycerides 1415-1539 mg / dL,

Phospholipid 1030.6-1079.3 mg / dL,

Total protein 12.0-12.8g / dL, Albumin 2.7-3.0g / dL,

Globulin 9.3-9.8 g / dL,

Blood biochemical analysis of experimental group 2

GOT 78 ~ 91U / L, GPT 6 ~ 7U / L, GGT 9 ~ 11U / L,

Total Cholesterol 140-157 mg / dL,

Triglycerides 332-425 mg / dL,

Phospholipid 745.4-637.2 mg / dL,

Total protein 5.7-6.2g / dL, Albumin 3.2-3.4g / dL,

Globulin 2.5-2.8 g / dL,

According to the above results, the GOT GPT level showed that the control group had the most severe damage to the liver, muscle, myocardium, etc., and the experimental group 1, which received the water-soluble ethoxyquine, was lower than the control group, indicating that the protective effect of the tissue cells was shown. Experimental group 2 showed the most stable, so the peroxidation reaction in the body is the lowest. This is direct evidence that exogenous fatty oxides are effectively detoxified in the body.

The level of GGT indicates the level of pathogenic bacteria infected with liver tissue cells, and the control group was the highest, and Experimental group 1, which received water-soluble ethoxyquine, had pathogenic bacteria infection in liver tissue cells. On the other hand, in Experimental Group 2, GGT was the lowest, and pathogenic bacteria due to the action of Eoseongcho and Seonhakcho seemed to be sterilized.

Triglycerides, Phospholipid, Total protein and Albumin were the lowest in the control group. It is a state in which nutrient metabolism is lowered due to peroxidation of hepatocytes by exogenous lipid peroxide, infection of perishable bacteria, and toxins secreted by bacteria. In experimental group 1, the level was too high, which is due to the inhibition of hepatic cell peroxidation by water-soluble ethoxyquine. However, the metabolic nutrients are stored in the blood due to the impaired circulation of peripheral blood vessels. Too high a value was recorded.

This is the reason for the desperate need for peripheral vascular dysfunction and free radical scavenger. Experimental group 2 is more metabolic nutrients in the liver cells than the control group and seems to be effectively transferred to peripheral tissues.

Scattering results are shown in Table 2 below.

TABLE 2

division Control Experimental Army 1 Experimental Army 2 % Scattering 68 68 82 Average egg weight (g) 61.1 64.2 64.2 Feed allowance (g / day) 119.9 121.4 126.6

In the control group and experimental group 1, the scattering rate was greatly reduced, but the experimental group 2 slightly decreased. In experimental group 2, the average egg weight was maintained despite the slight decrease in egg production.

<Example 2>

In order to experiment with pigs, 9 heads of 30kg Landrace species were divided into control, experimental group 1, and experimental group 2 for 100 days, and unlimited water and feed were administered during the breeding period. Experimental feed is a commercial feed that is commercially available.

Control: General feed.

Experimental group 1: 0.015% of water-soluble ethoxyquine was added based on 100% weight ratio of general feed.

Experimental Group 2: 0.015% by weight of water-soluble ethoxyquine, 0.01% by weight of Schisandra chinensis extract (98Brix), 0.003% by weight of Ginkgo biloba extract (98Brix), 0.0007% by weight of electric breeze extract (Meloilox extract) (98Brix) , 0.005% by weight of jinjin mugwort extract (98Brix), 0.015% by weight of fish vinegar extract (98Brix), 0.015% by weight of tricotweed extract (98Brix), 0.015% by weight of Seonhakcho extract (98Brix).

The extract was adsorbed onto adsorbent silica, converted to powder form, and then mixed and administered to feed. To verify the efficacy of the onset, blood biochemistry, body weight, and feed efficiency were compared at the end of the experiment.

Equipment used for blood biochemical analysis: CH-100 PLUS from SEAC, Italy, used as a reagent of Stanbio company, and the standard serum was calibrated for each analysis item and examined.

Blood biochemical analysis of the control group

GOT 84 ~ 97U / L, GPT 38 ~ 42U / L, GGT 35 ~ 38U / L,

Creatinine 1.8-2.1U / L, Total Cholesterol 109-116mg / dL,

Triglycerides 65-72mg / dL, Phospholipid 99.6-112.1mg / dL,

Total protein 6.2-6.8 g / dL,

Albumin 3.8-4.2 g / dL, Globulin 2.4-2.6 g / dL,

Blood biochemical analysis of experimental group 1

GOT 67-62U / L, GPT 29-33U / L, GGT 35-38U / L,

Creatinine 1.6-2.0U / L, Total Cholesterol 105-116mg / dL,

Triglycerides 210-232 mg / dL, Phospholipid 240-270 mg / dL,

Total-protein 9.8-10.1 g / dL,

Albumin 5.4-5.9g / dL, Globulin 4.2-4.4g / dL,

Blood biochemical analysis of experimental group 2

GOT 58 to 62 U / L, GPT 13 to 18 U / L, GGT 11 to 14 U / L,

Creatinine 0.6-0.8U / L, Total Cholesterol 107-114mg / dL,

Triglycerides 102-106mg / dL, Phospholipid 184-197mg / dL,

Total protein 7.8-6.2 g / dL,

Albumin 5.3-4.2 g / dL, Globulin 2.5-2 g / dL,

According to the above results, in the experimental group 1 to which the water-soluble ethoxyquine was added compared to the control group, the GOT GPT level was lowered due to the protective action of the hepatocytes, and the experimental group 2 showed more stable values. GGT showed no difference between the control group and the experimental group 1, but showed a large difference from the experimental group 2, indicating that the decaying bacteria were infected with liver tissue and had a significant toxic effect.

In terms of creatinine (Creatinine) levels in the control group and the experimental group 1 pigs showed a decrease in kidney function and damage, while the experimental group 2 shows a stable value. This is clear evidence that peripheral vascular hematopoiesis improved in pigs of Experimental Group 2.

In experimental group 2, which improved peripheral vascular dysfunction, free radical scavenging of blood, liver detoxification of exogenous lipid peroxide, and bactericidal bactericidal activity, the level of metabolic nutrients synthesized in liver was higher than that of control group and experimental group 1, but experimental group 1 It was lower than that.

This is because experimental group 1 is protected by hepatic ethoxyquine and liver function is restored, but metabolic nutrients are stored in blood because of peripheral vascular hemostasis.

The weight gain and feed intake of the raised pigs are shown in Table 3.

TABLE 3

division Weight before experiment (Kg) Weight at end (Kg) Weight gain (Kg) Feed intake Feed efficiency Control 29.8 108.7 78.9 2.46 3.11 Experiment group 1 30.2 110.8 80.6 2.48 3.08 Experiment group 2 29.9 113.9 84 2.52 3.00

As a result of experimental group 2, hepatocellular protection improves liver function, blood free radical scavenging and peripheral vascular thrombolysis improves peripheral blood circulation, sterilization of perishable bacteria and sterilization and infection of infected perishable bacteria Digestive capacity seems to be improved by improving the internal organ function of the liver and metabolic nutrients in the peripheral tissue metastasis increased the increase in reproductive rate can be seen that significantly.

Example 3

To test the dairy cows, two dairy cows producing grade 2 milk and two dairy cows diagnosed with mastitis were separately added to the concentrated feed, which was administered and administered as follows.

Prior to the experiment, several experiments were performed in advance to determine the most significant factors among the causes of frequent respiratory diseases and frequent diarrhea in dairy cattle and other cattle.

The ruminant shaft has a large amount of free radicals during nutrient metabolism.

To determine whether peroxidation of tissue cells in the body is induced by free radicals or peroxidation of tissue cells by exogenous fat oxides, the level of fat oxide contained in dairy cow milk is measured and grade 1-2 milk is used. The blood of the normal group producing was analyzed.

Dairy cattle feed analysis result

POV 43.8-40.2 meq / kg oil,

Anisidine (AnV) 31.13-27.82 mg / kfg oil,

Total Aflatoxin 17-19ppb, Histamin 70-105mg / kg feed,

Normal blood analysis results

GOT 70-103U / L, GPT 23-35U / L, GGT 35-39U / L,

Creatinine 0.8-1.0 U / L, Total Cholesterol 247-307 mg / dL,

Triglycerides 15-22mg / dL, Phospholipid 290-300.7mg / dL,

Total Protein 6.2-7.4 g / dL, Albumin 3.8-4.1 g / dL,

Blood analysis of mastitis syndrome

GOT 95-70U / L, GPT 27-36U / L, GGT 33-38U / L,

Creatinine 3.2-2.9 U / L, Total Cholesterol 148-270 mg / dL,

Triglycerides 15-21mg / dL, Phospholipid 260-331mg / dL,

Total Protein 6.6-7.2 g / dL, Albumin 3.1-3.8 g / dL,

As can be seen above, the POV level of dairy cattle feed is significantly lower than that of other livestock, and the fat content in the feed is low. It is judged that there is no action.

Due to the characteristics of feed materials, decaying bacteria, including mold, caused by oxidation of fat, are considered to be a high level in terms of aflatoxin and histamine, which support that GGT levels are high in both normal and mastitis groups. The big difference between normal and mastitis is creatinine, which indicates the level of kidney damage.

The mastitis group had a significantly higher level than the normal group, and the normal group also showed signs of mastitis frequently. It should be seen as damage and bacterial growth.

Therefore, in dairy cows and fattening, the compounding ratio of added substances should be greatly modified due to physiological characteristics.

Both normal and mastitis groups were added at the following ratios.

0.005% by weight of water-soluble ethoxyquinone, 0.015% by weight of Schizandra chinensis extract (98Brix), 0.003% by weight of Ginkgo biloba extract (98Brix) and 0.0007% by weight of Mellilotus extract (98Brix), relative to 100% by weight of rich feed (98Brix) 0.005% by weight, fish herb extract (98Brix) 0.015% by weight, three hundred seconds extract (98Brix) 0.015% by weight, Sunhakcho extract (98Brix) 0.015% by weight was added.

The effect was verified by observing the rate of change of somatic cells and blood after 4 weeks of thick feed added at the above ratio.

After three weeks of administration, blood biochemistry showed no difference in blood biochemical analysis between normal and mastitis patients.

(Normal group, mastitis group)

GOT 46 to 59U / L, GPT 15 to 18U / L, GGT 12 to 16U / L,

Creatinine 0.6-0.7 U / L, Total Cholesterol 206-235 mg / dL,

Triglycerides 23-27mg / dL, Phospholipid 330.4-340.3mg / dL,

Total Protein 6.9-7.4 g / dL, Albumin 3.4-3.9 g / dL,

The somatic cell change rate is shown in Table 4 below. (The somatic cell determination was performed every week.)

TABLE 4

division 1 week 2 weeks 3 weeks 4 weeks Normal group A Grade 2 Grade 1 Grade 1 Grade 1 Normal group B Grade 2 Grade 1 Grade 1 Grade 1 Mastitis Group A Disposal Disposal Grade 3 Grade 2 Mastitis group B Disposal Disposal Grade 3 Grade 2

According to the table above, GOT GPT levels are lowered, and total-Cholesterol, Triglycerides, Phospholipid, are stable in both normal and dermalitis groups. The stability of the free radicals and peripheral vascular dysfunction were improved, and metabolic nutrients were normally transferred to peripheral tissues.

Therefore, the damage of kidney tissue is improved and the function of kidney is improved (improving the ability of Creatinine to be excreted in blood). The mammary gland is due to the action of eliminating blood free radicals, eliminating inflammation, sterilizing rot bacteria, and improving peripheral blood circulation. The damage seems to be improving as it recovers.

Therefore, as time passed, the quality of milk changed to a stable trend in the normal group, and in the mastitis group, the decrease in somatic cell count was remarkable due to the resolution of inflammation.

Example 4

In order to experiment with farmed fish, 1000 full length 20C flounder fish were separated and housed in three tanks for 6 months.

The flounder was screened at 1,200,000 heads in Jeju seedling culture center and transported to the experimental farms when the rear end group reached 1500 cm in total length.

After the transfer, the (U.S. rangen) E.P feed was grown to 20 cm by administering vitamin EC and glutathione, and 1000 animals were selected and used for the experiment.

Experimental feed

Mackerel (raw feed) 150kg + powdered feed 10kg + fish meal 15kg,

Moisture 63.69%, crude protein 21.49%, crude fat 9.94%, ash 1.95%, carbohydrate 1.73% Indirectly judged by measuring histamine concentrations of fatty acid levels and the growth of perishable bacteria in mackerel and fish meal used as raw materials for experimental feed. It was.

TABLE 5

division Lipid Peroxide (POV) Ansidingar (AnV) Histamin Raw Mackerel 254-320mg / kg oil 269-380mg / kg oil 170-215mg / kg Fish meal (prime meal) 59-63mg / kg oil 41-43mg / kg oil 42-53mg / kg

As a result of POV and AnV, the level of fat oxide was considerably high, and the concentration of histamine (histamin) showed the proliferation of decaying bacteria.

Control group: Administered experimental feed,

Experimental group 1: experimental feed + vitamin E 500,000 IU / kg diet + vitamin C 1000 mg / kg diet,

Experimental group 2: 0.025% by weight of water-soluble ethoxyquine, 0.01% by weight of Schisandra chinensis extract (98Brix), 0.003% by weight of ginkgo biloba extract (98Brix) and 0.001% by weight of electric leaf extract (Melilotus extract) (98Brix) %, Phosphorus mugwort extract (98Brix) 0.005% by weight, fish herb extract (98Brix) 0.02% by weight, trichome extract (98Brix) 0.02% by weight, Seonhakcho extract (98Brix) 0.02% by weight is added.

Experimental group 1 carried out the same format as the wet feed was prepared in Jeju. Jeju Island's aquaculture farms were using vitamin E (500,000 IU / g) raw materials and the amount of addition was quite high.

Experimental group 2 was added in excess because the fat-soluble vitamins contained in the live feed were mostly oxidized and destroyed.

The result is as follows.

Control group:

Ocular proliferation and blackening occurred on the 30th day of administration and died on the 5th-10th day at 67th day. The external features of our individuals were severe exophthalmos, ascites, hernias, blackening, and ulcers near the fins.

At this time, the microbial medium test was performed and chloramphenicol was cliniced for 1 week. During the antibiotic clinic, 67 patients died. In the control group, it was necessary to stop the experiment due to mortality and antibiotic clinic. Before the antibiotic clinic, 3 healthy specimens were randomly examined and the internal organ levels were similar. Collected blood was analyzed and the mean value was recorded.

Blood Biochemistry Assay

GOT 649 IU / L, GPT 235IU / L, Total bilirubin 1.6mg / dL,

Triglycerides 17mg / dL, Total Cholesterol 106mg / dL,

Phospholipid 161.6mg / dL, Total Protein 6.4g / dL, Albumin 3.7g / dL,

Total globulin 2.7g / dL, A / G 1.37, Creatinine 0.9Units / L,

Experimental Group 1:

At 85 days after administration, the prominent eyeballs began to be seen, and 2-3 animals were killed every 3-4 days. Apparently, three randomly sampled healthy subjects were sampled and necropsy showed no congestion of liver tissue and no spleen swelling compared to the control group.

Some 20% of black spots developed on the back.

Blood Biochemical Analysis

GOT 539 IU / L, GPT 95 IU / L, Total bilirubin 0.4mg / dL,

Triglyceride 75mg / dl, Total Cholesterol 245mg / dl,

Phospholipid 242.2mg / dl, Total protein 8.4g / dL, Albumin 5.7g / dL,

Total globulin 2.7g / dl, A / G 2.11 Creatinine 0.8Units / L,

Experimental Group 2:

During the experimental period, there were 25 deaths in 180 days, and 15 deaths occurred every 2 to 3 days for 17 days. The deaths were stopped on the 17th day from the day of administration, and 1 to 2 deaths after the wave.

As a result of microbial media, pathogenic bacteria were not detected, and microscopic examination of kidney and spleen flakes revealed microscopic granules of ceroids and no granules on day 15.

At 180 days of administration, three animals were randomly arrested and sampled, blood was collected, and necropsy was taken.

At the end of administration, the average body weight was 300 to 450 g.

Blood Biochemical Analysis

GOT 80 IU / L, GPT 9 IU / L, Total bilirubin 0.4mg / dL,

Triglyceride 414mg / dL, total Cholesterol 348mg / dL,

Phospholipid 1080mg / dL, Total protein 4.9g / dl,

Albumin 2.1g / dl, total globulin 2.3g / dL, A / G 0.91,

Total Calcium 20.7mg / dL, Total Phosphorus 14.3mg / dl,

As a result of experimental group 2, there were no signs of toxicity of fat oxide or signs of toxicity by decaying bacteria in blood biochemical analysis.

Since the level of fat oxide is high in the diets of farmed fish, the primary toxicity was greater in the control and experimental group 1, and the secondary toxicity caused by the decaying bacteria led to frequent disease outbreaks. It appeared to die.

Therefore, the field of the present invention most effectively improves the symptoms can be said to be aquaculture fish.

As described above, according to the present invention, the method of detoxifying and sterilizing the fat oxide and the perishable bacteria in feed reduces the incidence of diseases by reducing the toxic effects caused by the fundamental quality problems of feed materials in the body, thereby reducing the incidence of feed nutrient metabolism and feed efficiency. On the other hand, it is possible to make imported livestock products and imported aquaculture products healthier and better quality.

Claims (2)

Water soluble ethoxyquine (100% of ethoxyquine component): 10% by weight, Non-Woundyl Dimethyl Dicarboxylate (100% Biphenyl-Dimethyl-Dicarboxylate Component): 5% by weight, Schizandra chinensis extract (98BriX concentrate): 5% by weight, Injin mugwort extract (98Brix concentrate): 5% by weight, Ginkgo leaf extract (98Brix concentrate): 5% by weight, Electric leaf extract (Melilotus extract) (98Brix concentrate): 5% by weight, Three hundred sec extract (98 Brix concentrate): 5% by weight, Echo extract (98Brix concentrate): 5% by weight, Seonhakchocho extract (98Brix concentrate): 5% by weight and Excipients: A composition for the detoxification of the fatty acid products contained in the feed and the sterilization of perishable bacteria in the body, characterized in that 50% by weight of the composition is added to the livestock feed or farmed fish feed. The method of claim 1, For the detoxification of the fatty acid products contained in the feed and the sterilization of decaying bacteria in the body, characterized in that the composition of the feed for livestock or farmed fish is added 0.001% to 0.25% by weight of the livestock or farmed fish feed Composition.