KR200371828Y1 - A Device for microbial respiration monitoring by turning injection system - Google Patents

Abstract

The present invention can simultaneously test several kinds of samples (wastewater), and it is possible to continuously supply oxygen to the reaction tank to prevent the interruption of the experiment due to oxygen depletion. The sample passed through the chamber is circulated back to the reaction tank to prevent the shortage of samples between experiments. Dissolved oxygen detection probes (S) are installed and the upper and lower ports (P1) and lower ports (P2), respectively, above and below. ) Is provided with a chamber for detecting the dissolved oxygen amount 10, a pump 20 for pumping a sample, at least two reaction tanks 30 and 30 'in which the sample is stored, and air in the reaction tanks 30 and 30'. To the air generator 40 for supplying gas, the pipes L1 and L2 connected between the chamber 10 and the pump 20 and the plurality of reactors 30 and 30 ', and the pipes L1 and L2. It is installed to alternately enter and discharge the sample of each reaction tank (30, 30 ') to the chamber 10 Control unit 50 and dissolved oxygen amount for controlling the operation of the plurality of on-off valves (V1, V2, V3, V4), the pump 20, the air generator 40 and the on / off valves (V1, V2, V3, V4) It includes a storage unit 60 for storing the data sensed by the detection probe (S).

Description

A device for microbial respiration monitoring by turning injection system}

The present invention relates to a microbial respiration rate measuring device, and in particular, it is possible to experiment simultaneously with several kinds of samples, and to enable continuous oxygen supply to the reaction tank to prevent the interruption of the experiment due to oxygen depletion, circulation and alternating of the sample The present invention relates to an apparatus for measuring microbial respiration rate by an alternating injection method in which a sample passed through a chamber, which is a dissolved oxygen amount detection unit, is circulated back into a reaction tank so that sample shortage does not occur between experiments.

Conventional microbial respiratory rate meter is divided into a method of calculating the respiratory rate of the microorganism by measuring the amount of oxygen reduction in the atmosphere and a method of calculating the respiratory rate of the microorganism by directly measuring the amount of dissolved oxygen in the sample.

In the former case, the microbial respiration rate can be measured for several samples at the same time, but it is difficult to implement a compact type device because it must be equipped with a separate counter and external oxygen communication for counting the amount of oxygen injection. Oxygen transfer rate varies depending on the reaction, making it difficult to detect stable oxygen consumption rate.

In order to overcome these disadvantages and obtain accurate data, the latter method of measuring the dissolved oxygen in the sample is used. In this case, one main reactor and the dissolved oxygen detector are used to measure the microbial respiration rate of one sample in one experiment. This is also possible, and it is also difficult to implement a compact type device because it requires a separate device for the detection of the measured data.

Recently, a plug-flow type microbial respiration rate measuring device has been developed. In this case, the respiratory rate of microorganisms was calculated by measuring the initial dissolved oxygen at the beginning of the reactor, passing the column of constant length, and measuring the final dissolved oxygen again, ie the oxygen consumption rate over time. However, even in this case, since the length of the column is fixed, when the microbial respiration rate is measured for the sample that has not been previously identified, the separate control method for preventing oxygen depletion in the column is not provided. If the speed is not smoothly controlled by the pump, oxygen consumption is depleted in the column before entering the dissolved oxygen detection unit.

In order to solve the conventional problems as described above, the present invention, first, to provide a compact structure and free movement of microbial respiratory rate measurement device, second, the present invention is convenient for the user to inject and set the sample for the experiment To provide a microbial respiration rate measuring device of the structure, third, to be able to experiment with a variety of samples within a limited time, and fourth, to enable continuous oxygen supply to the main reactor to prevent the experimental interruption caused by oxygen exhaustion It is possible to prevent the shortage of samples between experiments by causing the sample passed through the dissolved oxygen detection part to circulate back to the main reactor during the circulation and alternating flow of the sample.

In order to achieve the above object, the present invention provides a dissolved oxygen amount detecting chamber, in which a dissolved oxygen amount detecting probe is installed, and upper and lower ports are respectively provided; A pump connected to the lower port side of the chamber to pump the sample; Two or more reactors in which samples are stored; An air generator for supplying air to the reactor; A pipe connected between the plurality of reactors, a pump, and a chamber; A plurality of on / off valves installed in the pipe for alternately introducing and discharging samples from each reaction tank into the chamber; A control unit for controlling the operation of the pump, the air generator, and the on / off valve; It provides a microbial respiratory rate measuring device of the alternative injection method comprising a storage unit for storing the data sensed by the dissolved oxygen detection probe.

In the present invention, the dissolved oxygen detection chamber is formed between the upper and lower bodies that are separable from each other to facilitate washing, and the watertight means to prevent leakage of the sample to the outside of the chamber in the combined state of the upper and lower bodies. It is provided, and the stirrer is provided to have a form that can be smoothly supplied and discharged after the supply of the sample, the uniform dissolved oxygen in the chamber.

In addition, the reaction tank is further provided with a stirrer to uniformly supply oxygen throughout the sample.

1 is a perspective view showing the overall configuration of the respiratory rate measuring apparatus according to the present invention,

2 is a front view of the present invention,

3 is an exploded perspective view of a chamber for detecting dissolved oxygen,

4 is an enlarged cross-sectional view of a chamber for detecting dissolved oxygen;

5 is an enlarged cross-sectional view of the reactor;

6 is a comparison of dissolved oxygen and oxygen consumption rate graph,

7 is a typical oxygen consumption rate graph.

* Explanation of symbols for main parts of the drawings

10: chamber for detecting dissolved oxygen amount 11: clamping means

12: upper body 12a: ceiling surface

14: lower body 14a: bottom surface

15: probe mounting hole 16: agitator

17: watertight means 20: pump

30,30 ': Reactor 31: inner tube

32: diffuser 33: stirrer

34: lid 35: air supply pipe

36: drive motor 40: air generator

50: control unit 60: data storage unit (laptop computer)

L1, L2: Piping P1: Upper port

P2: Lower port S: Probe for detecting dissolved oxygen

V1, V2, V3, V4: On-off valve (solenoid valve)

L3, L4: Drain Piping

Hereinafter, with reference to the accompanying drawings, preferred embodiments that do not limit the present invention will be described in detail.

1 to 5 show the configuration of the microbial respiration rate measuring apparatus according to the present invention, the present invention is a dissolved oxygen detection probe (S) is installed in the upper and lower portions of the upper sample port (P1) and the lower port ( Dissolved oxygen amount detection chamber 10 provided with P2); A pump 20 connected to the lower port P2 side of the chamber 10 to pump a sample; Two or more reactors (30, 30 ') in which the sample is stored; An air generator 40 for supplying air to the reactors 30 and 30 '; Pipes L1 and L2 connected between the chamber 10 and the pump 20 and the plurality of reaction tanks 30 and 30 '; A plurality of open / close valves V1, V2, V3, and V4 installed in the pipes L1 and L2 for alternately introducing and discharging samples from the reaction tanks 30 and 30 'into the chamber 10; A controller 50 for controlling the operation of the pump 20, the air generator 40, and the on / off valves V1, V2, V3, and V4; It includes a storage unit 60 for storing the data detected by the dissolved oxygen detection probe (S).

Measuring device of the present invention is made of a moving booth (casting booth) attached to the caster to move as shown in Figure 1 is to be freely moved in the laboratory as well as to move it to another place In addition, the front door is opened to allow the operation of sample input / discharge into the reaction tanks 30 and 30 'or to observe the experimental state visually.

In the drawings, reference numerals L3 and L4 denote drain pipes connected to the lower ends of the reactors 30 and 30 ', respectively.

The chamber 10 is formed between the upper and lower bodies 12 and 14 which can be separated and coupled to each other by the clamping means 11, as shown in FIGS. The concave formed funnel-shaped floor surface 14a and the solid surface-shaped ceiling surface 12a inclined toward the center in a symmetrical form with the bottom surface 14a and the outer side surface 13 consist of a sample. The upper port P1 and the lower port P2 are formed at the center of the ceiling surface 12a and the center of the bottom surface 12a, respectively, and are connected to the pipes L1 and L2.

In the present invention, the bottom surface 14a of the chamber 10 has a funnel shape. The reason for discharging the sample that was filled in the chamber 10 for the detection of dissolved oxygen is to leave the lower port P2 without leaving the sample. In order to be completely discharged through, and the ceiling surface (12a) of the chamber 10 to form a solid form when the sample is injected into the chamber 10 to detect the dissolved oxygen amount of the sample It is to allow the sample to be filled in the chamber 10 by allowing complete exhaust of the air filled in the chamber 10.

In addition, the upper body 12 constituting the chamber 10 is formed with a probe installation hole 15 for installing the dissolved oxygen detection probe (S), the lower body 14 inside the chamber 10 A stirrer 16 is provided to stir the sample injected into the sample, and the sample inside the chamber 10 is stirred to distribute the uniform dissolved oxygen as a whole.

The upper and lower bodies 12 and 14 constituting the chamber 10 are made of a synthetic resin such as acrylic or polycarbonate, which is a transparent material, and in the coupled state of the upper and lower bodies 12 and 14 to the outside of the chamber 10. Watertight means 17 having an O-ring shape is installed around the upper surface of the lower body 14 so as to prevent leakage of the sample and is in close contact with the lower surface of the upper body 12.

The pump 20 is to inject a sample of the reaction tank (30, 30 ') into the chamber 10 and to recover the sample detected in the chamber 10 back to the reaction tank (30,30'), the forward and reverse rotation Use this possible specification.

Since the reactors 30 and 30 'both have the same structure, only the structure of the reactor 30 on one side will be described.

The reaction tank 30 containing the sample waste water is formed in a cylindrical shape with a lower end closed and an upper part open. The upper part of the reaction tank 30 includes an inner cylinder 31 and an acid diffuser 32 and an agitator at the bottom of the inner cylinder 31. The lid 34 provided with (33) is detachably covered, so that the sample is introduced into the empty reaction tank 30 in the state where the lid 34 is removed, and the lid 34 is covered.

An air supply pipe 35 connected to an air generator 40 to supply air to the diffuser 32, a drive motor 36 for driving the stirrer 33, and an upper portion of the lid 34. The pipe L1 connected to the upper port P1 of the chamber 10 is provided.

The air generator 40 is for supplying air to both reaction tanks (30, 30 ') of the operation is controlled by the controller 50.

On the other hand, the one side pipe (L1) is connected to the lower port (14a) of the chamber 10 via the pump 20 in the lower portion of the one side reaction tank 30 and again one side in the upper port (12a) of the chamber 10 It is connected to the upper part of the reaction tank 30. In addition, the other side pipe L2 is connected to the lower port 14a of the chamber 10 via the pump 20 at the lower part of the other reaction tank 30 'and is again connected to the upper port 12a of the chamber 10 at the other side. It is connected to the upper part of the reaction tank 30 '.

The both side pipes (L1, L2) has a part shared in part, the pump 20 is installed in the middle of the shared part.

In addition, two on-off valves V1, V2 (V3, V4) are symmetrically installed at portions not shared by the pipes L1 and L2, respectively, and on / off valves V1 and V2 provided on one side pipe L1. ) And the on-off valves V3 and V4 provided on the other side pipe L2 are made to have a mutually opposite opening / closing action. For example, when supplying a sample of one side reaction tank 30 to the chamber 10, the one side pipe L1 Since only the installed on-off valves V1 and V2 are opened and the on-off valves V3 and V4 installed on the other side pipe L2 are closed, the pump 20 is operated in one direction so that the sample of the other side reaction tank 30 'is mixed. It is to be prevented.

The on-off valve (V1 ~ V4) by using a solenoid valve that is operated in an electrically driven manner it is possible to automatically integrated control in the control unit 50 during the alternate injection and discharge of the sample.

A method for measuring the microbial respiration rate of two or more wastewaters having different properties by using the microbial respiration rate measuring device of the present invention configured as described above and a method for comparing the microbial activity or analyzing the COD fractionation Let's explain.

(Experimental method)

The influent COD fraction using respiratory rate measurement measures dissolved oxygen (DO) or oxygen consumption rate (or CO ₂ generation rate) in the gaseous state, and when using the device of the present invention, the experimental method is as follows.

1. Take samples (influent wastewater) and place them in two or more main reactors. At this time, the injection amount is sufficiently injected in consideration of the collected sample during water quality analysis (eg, COD analysis) that is performed simultaneously with the measurement of the microbial respiration rate.

2. Inject microorganisms (sludge) to be used in the experiment into two or more main reactors after pretreatment (preaeration or washing) according to the purpose of the experiment.

3. Control the controller 50 with the built-in software such as a pump and a valve and the stirring device in the chamber for detecting the dissolved oxygen amount by using the notebook computer 60 at the same time as the microorganism injection. Monitor DO change by time zone for alternately injected samples. (If necessary, take samples for water quality analysis through drain pipes (L3, L4) installed under each reactor (30, 30 ').)

4. The data entered into the computer is recorded at equal intervals according to the result of the program on the hardware (eg Excel file) and converted into graphing work.

5. From the data (data and graphs) thus obtained, perform microbial activity comparisons and COD fractionation.

(Get the COD fraction)

Get TCOD (Total COD)

To obtain the COD fraction, the TCOD value is obtained using non-filtered wastewater (NFWW). This is the basis for COD fractionation in wastewater.

Get Dissolved COD (SCOD)

Afterwards, the influent water (NFWW) is filtered using a membrane filter (0.45μm) (However, in the broad concept of excluding microbial effects without a membrane filter, GF / C can be used, but general solid matters are used.) In the concept of filtering it is more preferable to use a membrane). In this way, the COD of the filtered wastewater (FWW) is measured, which becomes the dissolved COD (SCOD) in the influent, and the COD (COD _P ) of the particulate component is calculated by the following equation (1). I can separate it.

COD _P = TCOD _i -SCOD ------------ Formula (1)

Find Ss with OUR

The filtered influent sample (FWW) is measured using the oxygen consumption rate (OUR) monitoring system to measure respiration rate as substrate utilization and growth. At this time, as the zoom Inject nitrification inhibitor ATU to be 20mg / L to see the oxygen consumption by heterotrophic microorganisms (Heterotrophs) using oxygen (Oxygen Uptake Rate) only, allows to eliminate the influence of the autotrophic microorganisms (Autotrophs) ( A ATU is injected during the OUR experiment at all COD fractions). At this time, Ss of the dissolved COD can be calculated by using Equation (2) below as a boundary (Endogenous level) at the point where the increased OUR is rapidly decreased.

Ss = OC ₁ / (1-Y _H ) ------------ Formula (2)

※ OC _One = Cumulative oxygen consumption (mg / L) due to the use of Ss

Find Xs with OUR

As shown in FIG. 6, the initial OUR section (indicated by ① in the figure) and the OUR trend (indicated by ② in the figure) hidden by Ss based on the OUR trend in the Xs interval obtained through the experiment are shown. Estimate (curve fitting) to calculate the total amount of oxygen consumed by Xs, and calculate the COD consumption by Xs using Equation (3) below.

Xs = OC ₂ / (1-Y _H ) ------------ Formula (3)

※ Cumulative oxygen consumption in the section due to OC ₂ = Xs use (mg / L)

Initial Microbial Concentration in Influent (X _H ) Get

The OUR test is performed using unfiltered influent (NFWW), but unlike the above, the influent is not planted by separate microorganisms. The initial microbial mass is obtained (however, in this experiment, ATU is injected to 20 mg / L).

When the OUR test is performed using NFWW (about 2 ~ 4 days), an increase in OUR due to the growth of microorganisms in the influent is shown, as shown in FIG. 7, which shows a sharp increase trend over time. do. Later on, when the dissipation of temperament is complete, there is a sharp drop in OUR.

At this time, the OUR appearing in the logarithmic growth interval of Figure 7 shows the exponential increase of the microorganism, by changing the exponential increase of the microorganism to a natural log, it is possible to estimate the initial microbial amount through the slope and Y-intercept . b _H is the decay value and can be obtained through separate experiments, but the value proposed by IWA (based on ASM1) is approximately 0.62 d-1 (at 20 ° C) and 0.2 d-1 (at 10 ° C). do. At this time, it should be noted that since the COD mg / L of the microorganism is different from the mg / L by the VSS, a correlation between a separate VSS and a COD should be obtained.

------------ Formula (4)

Non-Degradable Solids (X _I ) Get

Each value obtained through the above process can be divided into X _I components based on mass balance as shown in Equation (5) below.

TCOD _i = _I X - (S + _S S _{S I} + X + X _H) ------------ formula (5)

As described above, the microbial respiration rate measuring apparatus using the alternate injection method of the present invention has a plurality of reaction tanks connected to one dissolved oxygen detection chamber, and stored in these reaction tanks to analyze the degree of reduction of dissolved oxygen due to chemical reaction. Hazardous samples are alternately injected into the chamber to measure dissolved oxygen and then circulated back to the original reactor, and samples from other reactors can be injected into the chamber at the same time for several types of samples (wastewater) within a limited time. It is possible to experiment, and the air generator can supply oxygen to each reactor to prevent the interruption of the experiment due to oxygen depletion, and when the sample passed through the chamber, which is the dissolved oxygen detection part, is returned to the reactor By circulating, there is no shortage of samples between experiments and no additional facility is required. Therefore, since the equipment itself is simplified, it is easy to move, and the injection of the sample for the experiment and the setting of the experiment are convenient, which has a useful effect of measuring the microbial respiration rate more efficiently.

Claims (6)

Dissolved oxygen amount detection probe (S) is installed and the upper and lower upper port (P1) and the lower port (P2), respectively, dissolved oxygen amount detection chamber (10); A pump 20 connected to the lower port P2 side of the chamber 10 to pump a sample; Two or more reactors (30, 30 ') in which the sample is stored; An air generator 40 for supplying air to the reactors 30 and 30 '; Pipes L1 and L2 connected between the chamber 10 and the pump 20 and the plurality of reaction tanks 30 and 30 '; A plurality of open / close valves V1, V2, V3, and V4 installed in the pipes L1 and L2 for alternately introducing and discharging samples from the reaction tanks 30 and 30 'into the chamber 10; Control unit 50 for controlling the operation of the pump 20, the air generator 40 and the opening and closing valve (V1, V2, V3, V4); And Microbial respiratory rate measurement apparatus according to the alternate injection method comprising a; storage unit for storing the data sensed by the dissolved oxygen detection probe (S) (60). The method according to claim 1, The chamber 10 is a microbial respiratory rate measuring device by the alternate injection method, characterized in that formed between the upper and lower bodies (12, 14) that can be separated and coupled to each other by the clamping means (11). The method according to claim 2, The chamber 10 includes a bottom surface 14a having a funnel shape in which a center is concave, and a ceiling surface 12a having a solid shape inclined toward the center in a symmetrical form with the bottom surface 14a. The lower port (P2) and the upper port (P1) is formed in the center of the bottom surface (14a) and the center of the ceiling surface (12a), respectively, characterized in that the microbial respiratory rate measuring device by the alternate injection method. The method according to claim 2, The upper body 12 of the chamber 10 is formed with a probe installation hole 15 for installing the dissolved oxygen detection probe (S), the lower body 14 is a sample injected into the chamber 10 Microbial respiration rate measurement apparatus by the alternate injection method, characterized in that the stirrer 16 is installed for stirring. The method according to any one of claims 2 to 4, The upper and lower bodies 12 and 14 constituting the chamber 10 are made of a transparent material, and an O-ring to prevent leakage of a sample to the outside of the chamber 10 while the upper and lower bodies 12 and 14 are coupled to each other. Water-tight means for forming a microbial respiration rate by the alternate injection method, characterized in that interposed between the upper, lower bodies (12, 14). The method according to claim 1, The reaction vessel 30 is a cylinder type with a lower end sealed and an upper portion open. An upper portion of the reaction vessel 30 includes an inner cylinder 31 and an acid mechanism 32, an agitator 33, and the chamber below the inner cylinder 31. 10. The microbial respiratory rate measuring device according to the alternating injection method, characterized in that the lid 34 is provided with a pipe (L1) connected to the upper port (P1) of the 10 is detachably covered.