KR20030012563A - Feed compound for substituting antibiotic - Google Patents

Abstract

PURPOSE: A substitute feed composition for antibiotics is provided, thereby easily increasing immunity and health of domestic animals without using antibiotics. CONSTITUTION: The substitute feed composition for antibiotics comprises 0.1 to 10 wt.% of eggs containing IgY obtained from laying hens immunized by an antigen selected from the group consisting of F4:K88, F5:K99, F6:987P, F18 and F41. It further comprises 35 to 45 wt.% of corns, 20 to 30 wt.% of dried whey, 10 to 20 wt.% of soybean waste, 5 to 15 wt.% of soybean protein, 0.5 to 8 wt.% of spray-dried blood, 0.5 to 8 wt.% of fish protein powder, 1 to 5 wt.% of animal lipid, 0.5 to 5 wt.% of calcium phosphate, 0.05 to 3 wt.% of limestone, 0.05 to 1 wt.% of premix of vitamin and mineral, 0.01 to 3 wt.% of salt, 0.01 to 3 wt.% of lysine, and 0.01 to 3 wt.% of methionine.

Description

Feed composition for antibiotic replacement {FEED COMPOUND FOR SUBSTITUTING ANTIBIOTIC}

The present invention relates to a feed composition for replacing antibiotics, and more particularly, to a livestock feed composition prepared by mixing eggs containing antibodies capable of preventing and treating bacterial diseases in animals.

The use of antibiotics in pig feed has been reported to improve animal growth prevention and feed efficiency, as well as to prevent and treat disease in animals (Hays, VW 1977. Effectiveness of feed additive usage of antimicrobial agents in swine and poultry production.Office of technology assessment, US congress, Washington DC (eds: Hays, VW 1981.The Hays report.Rachel Laboratories, Inc., Long Beach, CA); Zimmerman, DR 1986. J. Anim. Sci . 62 (Suppl. 3) 6), since 1950, pig feed has been mixed with antibiotics (Cromwell, GL 1991. Antimicrobial agents. Swine Nutrition (eds: ER Miller, Ullery, DE and Lewis, AJ). Butterworth-Heinemann.P. 297 ).

However, as public health interest in antibiotic residues and antibiotic resistant bacteria in meat products has increased (Braude, R. 1978. J. Anim. Sci . 46: 1425-1436; Kunin, CM 1993. Ann.Intern . Med . 118). (557-561; Cassel, GH 1995. ASM News. 61 (3): 116-120), research is underway in the pig industry to develop antibiotic substitutes (Vanbelle, M. 1989. Biotechnology in the feed industry pp 191. Proc. of Alltech's 5th Annu.Symp.Alleth Tech.Publ., Nicholasville, KY.).

Since the new livestock is immunized by ingesting antibodies present in the colostrum of the mother, the early reason weakens the defense against the disease of the new livestock and greatly increases the incidence of disease.

It has been reported that diarrhea was completely prevented by oral administration of colostrum-free E. coli K88, 987P and K99 specific antibodies to fimbrial adhesin (Yokoyama, H., Hashi, T., Umeda). , K., Icatlo Jr, FC, Kuroki, M., Ikemori, Y. and Kodama, Y. 1997. J. Ver. Med. Sci . 59: 917-921).

Immunoglobulins in egg yolks can be used not only as a source of immunoglobulins, but also as food ingredients (Shimizu, M., Fitzsimmons, RC and Nakai, S. 1988. J. Food. Sic . 53 (5). ): 1360-1366). In particular, yolks or immunoglobulins are reported by the report that antibodies formed in the immunized laying hens of Rose et al. (Rose, ME, Orlans, E. and Buttress, N. 1974. Eur. The utility of was tested.

In addition, it was confirmed that subunit vaccines prepared by purely separating and purifying K88, K99, and 987P from enterococci E. coli have immunogenicity and antibody persistence in laying hens, and titers of specific immunoglobulins isolated from egg yolk were studied. Svendsen, L., Crowly, A. and Ostergaard, LH 1995.Laboratory Animal Science.45: 89-93; Kim, JW, Cho, SH and Koh, SY 1998.The 8th world conference on animal production. Contributed papers-Vol II.36-37; Kim, JW, Kim, C., Koh, SY and Cho, SH 1998b.The 8th world conference on animal production. Contributed papers-Vol. II.424-425; Kim Jung-woo, Kim Do-gyun, Kim Chul. 2000. Production of specific yolk antibodies against ciliated antigens of enterotoxic Escherichia coli K99 (F5), Korean Journal of Animal Science and Technology, 42: 371-378).

However, egg yolk antibodies have various antibody titers depending on the antigens inoculated into laying hens, and there are few studies on egg yolk antibodies against two or more antigens, and there are no studies on yolk antibodies on strains that occur frequently in domestic pig farms. to be.

An object of the present invention is to provide a feed composition capable of improving the health of an animal.

It is also an object of the present invention to provide a feed composition that can replace a feed comprising an antibiotic.

It is also an object of the present invention to provide a feed composition that can reduce the incidence of disease in animals.

In order to achieve the above object, the present invention comprises from 0.1 to 10% by weight of an egg obtained from a laying hens immunized with at least one selected antigen from the group consisting of F4: K88, F5: K99, F6: 987P, F18 and F41 Provide a feed composition.

Hereinafter, the present invention will be described in detail.

The present invention has developed a feed composition that can enhance the health of livestock and prevent diseases caused by pathogenic bacteria without including antibiotics.

Feed composition of the present invention comprises a yolk antibody in a conventional livestock feed composition. The egg yolk antibody is obtained from a laying hen immunized by a bacterium causing disease in livestock, and contains specific antibodies against the bacterium.

The conventional livestock feed composition is preferably all kinds of compositions used for livestock, in particular antibiotics are excluded from the feed containing the antibiotic. Most preferably it contains 3,340 kcal ME / kg, 15-25 wt% crude protein, 1-2 wt% lysine. For example, 35 to 45% by weight of corn, 20 to 30% by weight of dry whey, 10 to 20% by weight of soybean meal, 5 to 15.00% of soy protein, 0.5 to 8% by weight of spray dried blood meal, 0.5 to 8% by weight of fishmeal, animal 1-5% fat, 0.5-5% calcium phosphate, 0.05-3% limestone, 0.05-1% vitamin mineral premix, 0.01-3% salt, 0.01-3% lysine, and 0.01-3 methionine Composition comprising weight percent.

Bacteria causing disease in livestock are preferably all kinds of pathogenic bacteria and viruses, more preferably bacteria which are etiologies occurring in pigs, cows, dogs, cats, goats, goats and animals. For example, swine diseases include diarrhea, cholera, Japanese encephalitis, swine infectious gastroenteritis, Ozeski's disease, swine genital respiratory syndrome, swine atrophic rhinitis, swine mycoplasma pneumonia, swine pasturela pneumonia, swine coliformosis, swine monologue, toxoplasma disease, Exudative epidermal infection, swine salmonellosis, and the like.

The laying hen immunity to the pathogenic bacteria is carried out by a conventional egg yolk antibody manufacturing method, and the laying hens are immunized by injecting a specific antigen of live bacteria, inactivated bacteria, crushed bacteria, or bacteria. In addition, the laying hens can be immunized against a single antigen, and one or more antigens can be mixed to induce the hens. Preferred antibodies are against F4: K88, F5: K99, F6: 987P, F18 or F41, the five of which are inactivated by conventional vaccine production methods, or an antigenic protein that shows a fragmented fraction or serotype as an antigen. It is good to use.

The method for producing an egg containing a preferred specific antibody of the present invention is carried out by known techniques (Korean Patent No. 0280331, Korean Patent No. 0280332, Korean Patent No. 0280333).

Eggs containing specific antibodies are extracted only from immunoglobulins in eggs and mixed in animal feed, or eggs are mixed in animal feed to prepare livestock feed containing egg yolk antibodies.

When the eggs are mixed with animal feed, the eggs may be dried, powdered and mixed, or mixed into pellets. Most preferably, the eggs are spray-dried and mixed with 0.1 to 10% by weight of livestock feed. Although the mixing ratio is preferably adjusted differently according to the titer of the yolk antibody, the animal feed composition generally contains less than 0.1% by weight of egg containing the yolk antibody, and the effect of enhancing the immunity of the animal is significantly reduced, and the weight exceeds 10% by weight. If so, various problems may occur such as an increase in the production price of the feed, a decrease in economic efficiency, and a decrease in the palatability of the feed.

Livestock feed composition comprising the yolk antibody of the present invention can induce a preventive and therapeutic effect on pathogenic bacteria to livestock due to the yolk antibody, it is possible to maintain the health of the livestock without the use of antibiotics. In addition, the side effects of antibiotics can be prevented as well as simple and easy to improve the immunity of livestock.

Hereinafter, preferred examples are provided to aid in understanding the present invention. However, the following examples are provided only to more easily understand the present invention, and the present invention is not limited to the following examples.

Example 1

Egg preparations containing antibodies

Isolates and identifies cilia antigens from enterococci E. coli K88 and 987P, which cause diarrhea in young pigs in domestic pig farms, to immunize laying hens. To dry by spray drying using a spray dryer to prepare a spray dried egg. Specific yolk antibody preparation experiment was carried out according to known techniques (Korean Patent No. 0280331, Korean Patent No. 0280332, Patent No. 0280333).

Corn 40.70%, Dry Whey 25.00%, Soybean Meal 15.21%, Soy Protein 10.00%, Dry Dried Blood Powder 2.00%, Fish Meal 2.50%, Animal Fat 2.50%, Calcium Phosphate 1.30%, Limestone 0.15% To prepare a basic feed, 0.22 wt% of vitamin mineral premix, 0.20 wt% of salt, 0.15 wt% of lysine, and 0.07 wt% of methionine, and 0.1 wt% of spray dried eggs were mixed with the base feed to prepare livestock feed.

The basic feed is 3,340 kcal ME / kg, 22.00 wt.% Crude protein, 1.50 wt.% Based on the American National Standard Guideline (NRC. 1998. Nutrient requirements of swine (10th ed.) National Academy Press, Washington. DC). Contains lysine.

Example 2

Was carried out in the same manner as in Example 1, using a spray dried egg 0.2% by weight to prepare a livestock feed.

Example 3

Was carried out in the same manner as in Example 1, using a spray dried egg 0.3% by weight to prepare a livestock feed.

Example 4

Was carried out in the same manner as in Example 1, the spray-dried eggs were prepared livestock feed using 0.4% by weight.

Comparative Example 1

Corn 40.70%, Dry Whey 25.00%, Soybean Meal 15.21%, Soy Protein 10.00%, Dry Dried Blood Powder 2.00%, Fish Meal 2.50%, Animal Fat 2.50%, Calcium Phosphate 1.30%, Limestone 0.15% Animal feed was prepared by mixing 0.22 wt% of vitamin mineral premix, 0.20 wt% of salt, 0.15 wt% of lysine and 0.07 wt% of methionine.

Comparative Example 2

Antibiotics Apralan in Comparative Example 1 ^?? Animal feed was prepared by adding 20 (Korea Vetchem. Co. Ltd., Korea, Aplamycine) to 0.5 wt%.

Experimental Example 1

The livestock was exposed to 72 heads of three-way hybrid weaning pigs from Duroc × Yorkshire × Landace. The body weight was 5.04 ± 0.09 kg before the start of the experiment.

Specification test was carried out in the experimental animal breeding room attached to Dankook University, the livestock feed of Examples 1 to 4 or Comparative Examples 1 to 2 was provided to the weaning pigs in the form of powder, free vegetarian, water was freely eaten using an automatic water dispenser To make it possible. Body weight and feed intake were measured at the end of the study to calculate daily gain, daily feed intake, and feed efficiency (ratio of weight gain to feed weight). (Reference, ME: metabolic energy)

The results SAS (SAS. 1988. SAS / STAT . User, s Guide (Release 6.03). SAS Inst. Inc., Cary, NC., General linear model procedure) as was statistically, the average inter-significant (Duncan's multiple range test, Duncan, DB 1955. "Multiple range and multiple F test." Biometerics. 11: 142).

The results for weaning pigs fed livestock feed for 10 days are shown in Table 1.

Item Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Daily weight gain (g) 205 287 260 288 288 293 Daily feed intake (g) 339 419 370 385 392 387 Feed efficiency 0.605 0.685 0.703 0.748 0.735 0.757

As shown in Table 1, when 72 heads of weaned pigs were tested for 10 days, the daily weight gain (growth rate) increased as the yolk antibody was added, but Comparative Example 2 and the practice except Comparative Example 1 were observed. There were no statistically significant differences in weaning pigs that consumed animal feeds of Examples 1-4.

In the daily feed intake was shown that the feed intake of Comparative Example 1 was higher than the other treatment, but there was no significant difference in the treatment period.

There was no significant difference in feed efficiency between the Examples 1 to 4 treatments compared to the Comparative Example 2 treatments. However, Example 1 to Example 4 and Comparative Example 2 treatment group was improved feed efficiency compared to Comparative Example 1 treatment group (P <0.05). Feed efficiency increased with egg yolk antibody level.

Therefore, it can be seen that when the base feed is mixed with 0.1 to 0.4% by weight of the spray-dried powder of the egg containing the specific yolk antibody instead of the antibiotic, the growth rate of the weaning pig and the weaning pig are improved.

Experimental Example 2

Three days after the start of the specification test of Experimental Example, all treatments were challenged orally with 1 ml of enterococci E. coli K88 (6.0 × 10 ⁹ cfu / ml) and 987P (1.2 × 10 ¹⁰ cfu / ml), respectively, to induce diarrhea. I was. FC values (Sherman, DM, Acres.SD, Sadowski, PL, Springer, JA, Bray, B., Raybould, TJG and Muscoplat, CC 1983 on day 2 and 4 after challenge with E. coli K88 and 987P) Infect Immun 42: 656-658, normal minutes: 0, soft stool: 1, diarrhea diarrhea: 2, severe diarrhea: 3). The results are shown in Table 2 below.

Item Pig population Population causing diarrhea FC figures Day 2 Day 4 Comparative Example 1 12 6 (1.8) 0 (0.4) Comparative Example 2 12 2 (1.0) 1 (0.4) Example 1 12 2 (0.9) 0 (0.4) Example 2 12 4 (1.2) 1 (0.6) Example 3 12 2 (0.5) 0 (0.2) Example 4 12 1 (0.5) 0 (0.3)

On the second day of diarrhea, the incidence of diarrhea in the treatment group of Comparative Example 1 was 50%, and the incidence of diarrhea was 17% or less in the treatment groups of Examples 1, 3 to 4, and Comparative Example 3 except for the treatment group of Example 2. In addition, comparing the FC values on the day 4 after challenge with E. coli K88 and 987P, it was found that diarrhea stopped in most individuals with FC values of 0.6 or less in all treatments.

In the above results, the treatment of Example 4 was found to be significantly lower diarrhea induction diarrhea incidence, it was possible to prevent diarrheal disease, the treatment of the other examples also showed a diarrhea prevention effect similar to antibiotic mixed animal feed treatment, diarrhea development After treatment with antibiotics, it showed a slightly improved treatment effect. Therefore, it is possible to replace the antibiotic when 0.1-0.4% by weight of the egg-dried powder containing the egg yolk antibody is added to the weaning pig feed.

As described above, the livestock feed composition comprising the yolk antibody of the present invention can replace the use of antibiotics, and easily and simply improves the immunity and health of the livestock.

Claims (2)

A feed composition comprising from 0.1 to 10% by weight of an egg obtained from a laying hen immunized with at least one antigen selected from the group consisting of F4: K88, F5: K99, F6: 987P, F18 and F41. According to claim 1, wherein the feed composition is 35 to 45% by weight corn, 20 to 30% by weight dry whey, 10 to 20% by weight soybean meal, 5 to 15.00% by weight soy protein, 0.5 to 8% by weight of spray dried blood meal, fish meal 0.5-8 wt%, animal fat 1-5 wt%, calcium phosphate 0.5-5 wt%, limestone 0.05-3 wt%, vitamin mineral premix 0.05-1 wt%, salt 0.01-3 wt%, lysine 0.01-3 wt% Feed composition further comprises 0.01% to 3% by weight, and methionine.