KR20020086812A - A new Myrothecium roridum Tode ex Fries F000252(KCTC 0980BP), and its use - Google Patents

Abstract

PURPOSE: Provided are a new Myrothecium roridum Tode ex Fries F000252(KCTC 0980BP) which shows herbicidal activity to paddy weeds, and its use as a biocontrol agent. CONSTITUTION: The new Myrothecium roridum Tode ex Fries F000252(KCTC 0980BP) is characteristically separated by collecting a soil sample and dilution plating the sample on a potato dextrose agar medium or a Littman oxgall agar medium and separating a microorganism showing in vivo and in vitro herbicidal activity to paddy weeds. The biocontrol agent comprises the Myrothecium roridum To de ex Fries F000252(KCTC 0980BP), its culture liquid or extract, and an agriculturally acceptable carrier.

Description

A new Myrothecium roridum Tode ex Fries F000252 (KCTC 0980BP), and its use}

The present invention relates to Myrothecium roridum Tode ex Fries KF000252 (KCTC 0980BP) and uses thereof, and more particularly, to the mythocysis of the mycoccus myopycete (Hyphomycete) The herbicidal activity of Myrothecium roridum Tode ex Fries F000252 (KCTC 0980BP) strain, its culture or its extracts against non-weed weeds such as field weeds such as black nymphs, silkworms, skewers, buckthorns and shamrocks The present invention also relates to a new strain Myrosium loridium F000252 (KCTC 0980BP) and its use which can be used as a biocontrol agent by having a control effect on plant diseases.

Weed species in agricultural ecosystems are very numerous and the damage is enormous. Weeds are an important limiting factor in reducing crop productivity by competing with crops for the moisture, nutrients, and sunlight that are essential for crop growth. Although various types of organic synthetic herbicides are currently used for weed control, the production loss of crops by weeds is still high, which is due to the ecological changes of weed colonies, herbicide resistance, and the current herbicides. This is because new weeds are difficult to control. Until now, the development of new microbial herbicides with low toxicity and environment friendliness has been required due to the serious pollution and human toxicity problems caused by the large use of organic synthetic herbicides for weed control. As a result, more than 100 kinds of microorganisms (mostly fungi) have been found to have a weed control effect in the past 30 years, and about 10 kinds of microbial herbicides are currently registered and used. In recent years, the control of dominant weed species by traditional methods has reached its limit, and the necessity of using biological control methods in parallel with the seedling method has emerged. In terms of preservation of diversity, it is a very appropriate control method.

As reported in connection with the development of microbial herbicides using the current pathogenic microorganism strain Sickle Pod (sicklepod) control as Alterna Ria Cassia (Alternaria cassiae), Spud cyano is (spurred anoda) as for the control Alterna Ria MACROSS Fora (Alternaria for macrospora ), Alternaria sp. for Italian thistle control, Colletotrichum malvarium , velvetleaf for control of prickly sida Fusarium lateritium for the control, Texas gourd Fusarium solani for the control, Phyllosticta sp., The citrus field for the control of the Johnsongrass. milk Weed bar (milkweed vine) as a control for the pie Saratov ttora palmitate Bora (Phytophthora palmivora), Colle Saturday as discussed leguminous plants for the Aeschynomene indica (Aeschynomene) control Tree Qom global Eos Forest cucumber Death (Colletotrichum gloeosporioides), azelaic Tina Lipa Ria (Ageratina riparia) As for controlling Yen Thai Roma teenager Latina (Entyloma ageratinae), Acacia live League or (Acacia saligna) As for controlling flow My Cloud Stadium ( For the control of Uromycladium and Cryptostegia grandiflora , Maravalia cryptostegiae and the like are used.

On the other hand, in Korea, a microbial herbicide has been patented to control the alligator , one of the weeds using Epicoccosorus strain, and Exero Hill Room to control the most problematic blood among the weeds. A study is being conducted to isolate and use a monosera strain ( Exserohilum monoceras ). In addition, the development of biologics using Plectosporium tabacinum for the control of peel and snare has been attempted. In 1991, ill lesions showing symptoms of late blight were collected to isolate Phytophthora erythroseptica late blight strains, and the spores of the late blight were re-inoculated to observe the incidence and suggest potential as biologics. I've done it. Recently, the Korea Research Institute of Bioscience and Biotechnology has been able to effectively control several types of field weeds, such as shamrock ( Trifolium repens ), Solanum nigrum , Aeschynomene indica , Xanthium strimarium , and Calystegia japonica . Oxalicum strain (Penicillium oxalicum ) strains have been selected and developed as a microbial herbicide for grasslands , and shows the possibility of application as a microbial herbicide for field weed control.

Meanwhile, the US Department of Agricultural Research (ARS) Weed Research Team is using a Myrothecium verucarria strain isolated from the problem weed, Sicklepod , to trouble the tomato growing region in the southwestern United States. To control. In addition, it has been applied to the control of purslane and tropospheric weed species, which are very problematic in tomato cultivation using myrosium bacteria. In addition, research is being conducted to control weeds by combining fungi such as Fusarium solani and Colletotrichum truncatum . Such microbial herbicides have been accepted as a natural control method that can replace methyl bromide, a conventional synthetic herbicide. In addition, in 1997, USDA's Yang and Brenner suggested the possibility of weed control by investigating the pathogenicity and host range of three Myrothecium sp. Strains against Amaranthus retroflexus . However, there have been no attempts to control biological weeds using myrosium bacteria in Korea.

Therefore, the present inventors, while researching and developing a method for controlling the major domestic weed species by biological methods using soil-derived microorganisms, separated the new strains showing a high herbicidal effect on the domestic weed species, the identification result I confirmed it was Lori Doom. The present inventors have completed the present invention by inoculating the strain itself (spores, etc.) to obtain the herbicidal activity effect, as well as confirming the effect of weed control and plant disease control using the bacterial culture solution and its extract.

Therefore, the present invention is that the Myersium Lorydum F000252 (KCTC 0980BP) strains belonging to the Hypomycete fungi exhibiting selective herbicidal effect against weed species are provided as an environmentally friendly microbial agent for paddy and field weed control. Of course, the purpose of the control of weeds and plant diseases using the culture solution and culture extract.

Figure 1 is a photograph showing the small spore spore morphology of the myroseceum Loridum F000252 (KCTC 0980BP) strain.

Figure 2 is a photograph showing the microbial morphology of the myrosium loridium F000252 (KCTC 0980BP) strain.

Figure 3 is a graph showing the mycelial growth according to the temperature and water activity conditions of the myrosium loridium F000252 (KCTC 0980BP) strain.

Figure 4 is a graph showing the herbicidal activity against Lemna paucicostata ( Lmna paucicostata ) of the mycecium Loridum F000252 (KCTC 0980BP) strain culture (the herb index is in the range of 1 to 5, 5 represents complete inhibition).

5 is a graph showing the herbicidal activity of the extract of the myrosium Loridum F000252 (KCTC 0980BP) strain culture extract against the frog frog rice (herb index is in the range of 1 to 5, 5 represents complete inhibition).

Figure 6 is a photograph showing the seed germination inhibitory activity by radianoclover epithelial infection of the myroseium Loridum F000252 (KCTC 0980BP) strain on a petri dish (wet).

FIG. 7 is a photograph showing that the radinoclover was severely epithelially infected by the mycecidium lorydum F000252 (KCTC 0980BP) strain.

Figure 8 is a photograph showing the cotyledon invasion process by radianoclover epithelial infection of the myroseceum Loridum F000252 (KCTC 0980BP) strain.

Figure 9 is a graph showing the in vivo herbicidal activity range for the field weed species of the myroseium Lori dom F000252 (KCTC 0980BP) strain culture.

FIG. 10 is a graph showing the in vivo herbicidal activity range for nonweed weeds of Myrosium loridium F000252 (KCTC 0980BP) strain culture.

The present invention is characterized by the new strain Mirosedium loridium F000252 (KCTC 0980BP).

In addition, it was confirmed that the myroseceum Loridum F000252 (KCTC 0980BP) strain, its culture solution and its culture extract have herbicidal activity and plant disease control effect, and include the potential as biopesticides.

Referring to the present invention in more detail as follows.

In order to explore new microorganisms for weed control, many soil samples are collected from all over the country, and dilution is carried out from the collected soil samples using potato dextrose agar medium and Litman oxgall agar medium. Bacteria were isolated by plating method, and weeds were tested for in vitro and in vivo herbicidal activity, and strains showing high herbicidal activity were selected.

The herbicidal assays were primarily selected for specific and highly herbicidal strains through in vitro bioassays such as Lemna paucicostata and Ladino clover. In vivo pot experiments were conducted to evaluate the potential for biopesticide development.

Referring to the identification and culture of the strain is as follows.

end. Isolation, Culture and Identification of Strains

1) Isolation and Identification of Bacteria

Isolation and identification of isolated fungal strains were carried out using potato agar, Potato dextrose agar, Litman's oxgall agar and malt extract agar. Dematiaceous Hyphomycetes [M. By B. Ellis, Compendium of Soil Fungi [D. By M. Anderson, Soil and Seed Fungi [T. See Watanabe et al. In particular, the morphological characteristics of sporodochia, conidiophore, phialide, phialoconidia, and mycelia of the present strains are identified after optical microscopy. .

2) Culture Characteristics of Bacteria

Mycelial growth of isolated bacteria is investigated using potato dextrose agar and malt extract agar medium. In addition, the liquid culture medium to be used for in vitro and in vivo activity assays was incubated at 25 ° C. for 7 days using potato dextrose broth and yeast-peptone-sucrose solution (YpSs). Use it. That is, in liquid culture, potato dextrose broth medium containing 20% potato and 10% soluble starch (pH 7.2), coarse soy flour 25%, gravy 1%, yeast extract 4%, NaCl 2%, K ₂ East-peptone-sugar solution medium (pH 7.2) containing 0.25% HPO ₄ , 2% CaCO ₃ is used. For the preparation of the culture, two 500 ml baffled flasks (50 ml each) were sterilized for 20 minutes at 121 ° C, and the agar plugs of the strain were inoculated at 28 ° C, Shake culture is used for 2 days at 150 rpm. In solid culture, pour 120 ~ 200 ml of sterile water into 200 g of wheat bran to completely soak the bran into water, and then put it in a Erlenmeyer flask (1 L) for 30 minutes at 121 ° C, cool it thoroughly Inoculate. Thereafter, incubated at 25 ° C. for 10 to 14 days to ensure sufficient spore formation.

I. Production of inoculator

Inoculum is obtained from a liquid culture and a solid culture using a carrier substrate. First, the liquid culture medium uses yeast-peptone-sugar solution medium (YpSs) (pH 7.2) as described above. The liquid culture inoculation method is a method of using a culture solution obtained after inoculation after inoculation of bacteria, and a culture solution itself containing a considerable amount of bacteria, such as mycelia and spores, as well as metabolites of bacteria is used as the inoculum. For inoculation, the mycelium and pellets in the culture medium are changed to a certain size so that they can be sprayed through a nebulizer, and a small amount of tween 20 or the like is mixed to spread the bacteria easily. To be able.

Secondly, a preparation made of a carrier such as bran is used as an inoculum. In order to maintain safety and continuous herbicidal activity in the packaging, it is formulated with a microbial herbicide using an agriculturally acceptable carrier. Examples of carriers for biopesticide formulation may include rice bran, wheat bran, barley bran, straw, barley straw, rice straw and the like. Bran solid medium adds specific nutrients to bran and adjusts the substrate: moisture ratio to 3: 5, 4: 5, 5: 5 (v / v), inoculates the bacteria, and adds gelatin to the cultures using corn starch. It is added by mixing and coagulation, and then formulated by pulverizing and drying. In summary, the composition contains relatively inexpensive and simple compositions, such as spores of wheat microorganism and 25% of wheat bran, 2.5% of corn starch, and 0.9% of agar. The microbial spore concentration range of the microbial herbicide uses 10 ⁵ to 10 ¹⁰ spores per gram, and the effective concentration generally used is 10 ⁵ to 10 ⁸ spore concentrations per gram.

In the present invention, when used as a microbial herbicide, it is appropriate to use 80 to 90 g of microbial herbicide per 1a by calculating the amount of spores of the myrosium loridium strain as 10 ⁸ spores / g.

Third, the culture extract is obtained by solvent extraction with ethyl acetate using the liquid culture medium, concentrated again under reduced pressure, and lyophilized. In the present invention, the extract is concentrated, and the concentrate is adjusted for each concentration of 0.1 to 100 ppm to perform a Lemna assay or the like.

All. Herbicidal activity assay

In vitro and in vivo activity assays were performed on 14 weed species using small pots.

1) In vitro activity assay

In vitro herbicidal activity of the isolates was carried out by remna assay and ladino clover seed germination. First, remna analysis is a kind of moth frog rice, assay using a mini plant rem or fausciuta. Diluting strain culture or extract by concentration, it is placed on a 24 well plate in which remna grows. How to investigate. The herbicidal activity index has a repair score of 0 to 5 and is determined by the rate of growth inhibition of Lemma. That is, the activity index is as follows.

<10% Growth Inhibition: 0, 11-30% Growth Inhibition: 1, 31-50% Growth Inhibition: 2, 51-70% Growth Inhibition: 3, 71-90% Growth Inhibition: 4, 91-100% Growth Inhibition : 5>

On the other hand, the herbicidal activity using remna is assayed in sterile Hutner's nutrient medium. Secondly, radianoclover seed germination method is to inoculate the seed on the wet plate and inoculate the bacteria on the wet plate, and to check the seed germination and seedling height, and induce seed germination in 3-4 days in advance. This is a method of inoculating bacteria directly to seedlings. That is, three filter papers were immersed in sterilized water and placed on a plate, and then the pathogen spores (spore suspension diluted in a sterilized water solution containing 0.1% Tween 20) were placed at a concentration of 10 ⁵ to 10 ⁸ per ml. It is treated by concentration and dentured in a humidified incubator.

2) In vivo pot activity experiment under greenhouse conditions

In order to select strains with pathogenic and herbicidal activity in weeds, the primary screening is the target weeds (sorghum, dolpi, wheat, barley, American larvae, crows, larvae, tabby, lizard, buckwheat, barley, dolpi, tusks and shamrock The leaves of) are assayed by directly treating the culture medium or spore suspension with pots. In other words, the microbial culture solution is used to grind fine enough to pass through the nebulizer using a homogenizer without filtration and used for screening while being stored in the refrigerator until drug treatment.

Plants for in vivo herbicidal activity assay should be mixed with an appropriate amount of fertilizer in unsterilized sandy soil, and then placed in a test pot (350 cm ² ) to make sowing spheres, sowing weeds or crop seeds, and covering them with fine soil. Discuss growing up in the greenhouse. The culture to be treated (0.1% Tween 20 solution) is treated with a hand sprayer and treated with foliage.

After treating the culture solution, the herbicidal efficacy of 4 and 14 days after the treatment for 2 weeks in the greenhouse is investigated by morphological and physiological observations. Treatment is divided into pre-emergence and post-emergence to examine herbicidal activity. In other words, 0% of uncontrolled and 100% of complete control were used to evaluate the degree of herbicidal activity. If it had a grade of 70 or higher, it was regarded as having a herbicidal effect on the plant. Seeds that are indicated as are considered controllable species.

All. Characteristics as plant disease control

The activity assay method in vivo is as follows according to the control bacteria.

?? Rice Blight (RSB): Pyricularia oryzae spores (10 ⁶ spores / ml) are spray-inoculated enough to flow down the rice Nakdong rice (two to three leaves). The inoculated rice is left in the dark for 24 hours, and then incubated in a constant temperature and humidity room with a relative humidity of 80% or higher at 26 ° C for 5 days, and then examined the area of disease.

?? Rice leaf beetle blight: After inoculating the hyphae mass of cultured Rhizoctonia solani into small pieces, the inoculated evenly in the pot where Nakdong rice of 3-4 leaf stage treated with medicinal treatment was incubated for 1 day in a wet room (25 ℃). After 4 days in a constant temperature and humidity room with a relative humidity of more than 80%, the diseased area of the leaf sheath was investigated.

?? Tomato and Pepper Plague: Spore Concentrations of Phytophthora infestans and Phytophthora capsici were made at 10 ⁵ sporangia / ml and cold treated at 13 ° C. for 2.5 hours. After spilling the strainer, sprayed inoculated to the pharmaceutical treated tomato and pepper seedlings (second to third leaf). Tomato seedlings were treated for 48 hours in a 20 ° C wet room and then incubated for 3 days in a 20 ° C constant temperature and humidity room (relative humidity above 95%), and then examined the area of disease.

?? Wheat rust: Puccinia recondita is a live parasite, so it is used in the laboratory for direct passage to plants, using seedlings formed in wheat seedlings as inoculum. Sowing seeds (light) of 5 grains in a disposable pot (6.5 cm), treated with seedlings seedlings grown for 8 days in a greenhouse, air-dried for 1 day, and then sprayed with a spore suspension (spore 0.67 g / liter) do. Inoculated wheat seedlings are incubated for 7 days in a constant temperature and humidity room of 20-23 ° C. and a relative humidity of 70%, and the lesion area is examined.

?? Barley powdery disease: Erysiphe graminis f. Sp.hordei is an absolute fungi parasite, so the spores formed on barley seedlings are used as the inoculum during direct passage to plants in the laboratory. Five barley seeds are seeded in a disposable pot (6.5 cm) and treated with barley seedlings grown in a greenhouse for 8 days, air-dried for 1 day, and then sprayed with a spore suspension. Inoculated barley seedlings are incubated for 7 days in a constant temperature and humidity room with a temperature of 18 to 20 ° C. and a relative humidity of 70%.

Hereinafter, the present invention will be described in more detail based on the following examples, but the present invention is not limited to the following examples.

Example 1: Isolation, Identification and Culture of Mycobacterium myrosesium Rorydum F000252 (KCTC 0980BP)

The present inventors collected more than 100 soil samples from all over the country to search for new microorganisms for weed control, potato agar (potato dextrose agar) and littman oxgall agar (Littman oxgall agar) from the collected soil samples 1,000 strains were isolated by dilution plate method. The isolated fungi were selected first through the in vitro herbicidal activity assay using rem, faucostata, and radinoclover seed germination methods, and the potential for microbial product development by performing in vivo pot experiments using selected strains. Final selection was made as candidate strains.

Referring to the identification and culture of the strain is as follows.

end. Isolation and Identification of Strains

Identification of isolated fungal strains was carried out using potato agar (potato dextrose agar) and malt extract agar medium. Morphological characteristics of isolated bacteria, such as conidiophore, sporodochia, small spore (phialide), and small spore spore (phialoconidia) were investigated under an optical microscope (50-400 magnification). As a result, this active bacterium was identified as myrosium bacteria, which are frequently found as parasitic or by-products in nature, and mycelial with diaphragm developed well and was an incomplete fungus belonging to the hypomycete river, Myrosium spp. . The strain according to the present invention was characterized by the formation of up to 1.5 mm or more sporodochia on the plate containing the medium over the culture period. The conidia were cushioned, initially dark green on mycelia, gradually black, and often combined with the conidia. In particular, the hard and dark conidia formed well in the dry state. Conidia were semitransparent to colored, forming dense branches and broom-shaped conidia. The spore mass was viscous, initially green, and gradually black. The conidia sporadized at the tip of the small bottle located above the conidia. The small bottle size ranged from 8.5 to 15.3 × 1 to 2 µm and the small spore size ranged from 6 to 8 × 1.5 to 2.5 µm. The virulent spores were translucent to black, single cell, ovoid to elongate. Based on the above results, this active strain belongs to imperfect fungi, which is widely classified as an imperfect fungi, forms hyphae of the diaphragm well and has spores on the structure such as conidia. (I) identified as the mycecidium lorydum species belonging to the fungus. The new strain Myrosium Lolidium F000252 according to the present invention was deposited on March 31, 2001 with the Korea Biotechnology Research Institute Gene Bank and was granted accession number KCTC 0980BP.

I. Fungal growth according to environmental conditions

The isolates were examined for mycelial growth by varying the temperature and water activity conditions on potato agar and malt agar medium. As a result, the growth of this strain was the best at 25 ℃ and was relatively good even at a relatively high temperature (30 ℃), mycelial growth was well even at low temperature (18 ℃) did not show a significant difference from the optimum temperature 25 ℃. In addition, unlike the water activity conditions, wort agar; generally of whether cultured on (MEA malt extract agar) investigated the mycelial growth after 10 days results, were the mycelial growth of the well at a high water activity 0.995 a _w 0.964 a _w Mycelial growth was generally good even under low moisture conditions, but almost no mycelial growth was achieved at the lower 0.95 a _w [Fig. 3]. The formation of conidia spores was well formed near 20 ~ 25 ℃ rather than at high temperature, and was observed to form better when there is a temperature change (under low temperature rather than high temperature) during the culture period. In the early stage of culture, white to light fleshy mycelia were formed well on the medium, and pigments were hardly secreted. The color on the back of the plates was fleshy, and when the scratched or agaplug was applied to the mycelia on the plate, It was shown that the formation of conidia spores, including the conidia of sex, was induced rapidly. On the other hand, the liquid culture was weakly red when shaken on potato agar medium, and the viscosity of the culture was higher than that of malt extract broth medium.

Example 2: in vitro Herbicide Activity Survey

Herbicide activity against Lemna paucicostata was assayed using the culture solution and the culture extract obtained in Example 1. Both the culture medium and the culture extract showed high activity against the remnant Pauschostata, the test organism used in the assay [FIGS. 4, 5]. In vitro culture of remnant faux costata was adjusted to 50,000 to 0.8 ppm and herbicidal activity (range of 1 to 5) was examined. In addition, the herbicidal activity index 2 showed very high activity even at 1,250 ppm, and the ethyl acetate extract of the culture broth showed very high herbicidal activity [3. On the other hand, the number of grams of the acetone extract of the type cultures negative and positive bacteria and Aspergillus (Aspergillus), candy Ranges (Candidus) bacteria or the like did not show nearly activity.

Example 3: Investigation of herbicidal activity on clover seeds and seedlings

Inoculation of the small bottle spores of the strain according to the present invention (concentration of 1 × 10 ⁷ small bottle spores per ml) before the seed germination was inoculated into the wet-treated plate of radiano clover, and the degree of inhibition of radinoclover seed germination and inhibition of cotyledon growth was observed. As a result, seed germination was remarkably suppressed even after 3 to 4 days of treatment, and growth of radinoclover seedlings was very suppressed even when treated with germinated seedlings. Investigation of the invasion process of myrosium bacteria was observed by microscopic and microscopic observation of bacterial infections on the seed epidermis and cotyledon after 2 days, and dark green-black conidia on the seed and cotyledon after 4-5 days. (sporodochia) and mycelia were well formed, and the growth of clover was greatly inhibited as the stem and roots progressed in addition to the seed and cotyledon [Figs. 6, 7 and 8].

Example 4: in vivo Herbicide Activity Survey

In vivo , we tested the activity of various weed species directly on plants and tested their activities. As a result, weeds on field weeds such as crows, larvae, scallops, skewers, buckthorns and shamrocks and paddy weeds, etc. The herbicidal effect was over 95%, and the herbicides of 70 and 80% were found in the varieties and the slivers respectively [Figs. 9 and 10]. The herbicidal activity appeared rapidly after several days of treatment and there was no significant difference in herbicidal activity level after 4 and 14 days. On the other hand, the herbicidal activity against sorghum, a kind of crop, was very weak against mature plants, and the effect was very weak when treated with seeds. In addition, the damage to crops was weak with respect to the culture stock solution for vegetables such as tomatoes, cucumbers and wheat barley plants. Therefore, the present microbial preparations can be effectively used for the control of weed species occurring in paddy fields such as watering canes, weed species generated when growing vegetables, and shamrock on the lawn.

Example 5 Herbicide Effect of Microbial Herbicide

Microbial herbicides formulated in the same manner as in the above examples are suspended in sterile water at concentrations of 1 × 10 ⁵ , 1 × 10 ⁶ , 1 × 10 ^7, and 1 × 10 ⁸ spores / g and treated to weed species including shamrock The herbicidal action was observed after 1-2 weeks. The herbicidal action of the strain was evaluated by similar criteria as in Experimental Example 1 above. The results are shown in Table 1 below.

From the results of Table 1, the mycobacteria have high herbicidal activity against various field weeds and some non-weeds, in particular, field weeds such as camellias, silkworms, doemari, buckthorns, shamrocks, and non-weeds such as seaweed. It showed high selective herbicidal effect.

Example 6: in vivo Investigation of plant disease control activity in pot

Plant disease control experiments on in vivo pots showed no damage to rice crops, especially rice crops, and the control effect on blast bottles was generally high. , More than 70% control effect. In addition, this strain showed 100% control effect when the stock solution was used to control wheat rust and barley flour disease, and high control effect of 80% even at 1/8 dilution concentration [Table 2].

As described in detail above, the present invention isolates and identifies excellent mycobacteria exhibiting specific herbicidal activity against the weed species of Myrosium spp. Loridum F000252 (KCTC 0980BP) strain, and cultured the field weeds and paddy field weeds. It was found that it exhibits strong herbicidal activity against the species. Therefore, the new strain can be used as a biopesticide for environmentally friendly weed control, less harmful to crops and has a very useful effect in controlling domestic problem weed species such as single-leaf and broad-leaf weed species. In addition, it was confirmed that the culture solution and the culture extract of the present strain can be effectively used for the control of the weed species.

Claims (5)

Myrothecium roridum Tode ex Fries F000252 (KCTC 0980BP), which is effective as a biopesticide. Herbicides characterized in that it comprises a Myrosium Loridum F000252 (KCTC 0980BP) strain, its culture or its culture extract and an agriculturally acceptable carrier. The herbicide of claim 2, wherein the herbicide is applied to field weed or non-weed weed. Plant disease control agent, characterized in that it comprises a myrosium Loridum F000252 (KCTC 0980BP) strain, its culture or its culture extract and an agriculturally acceptable carrier. 5. The plant disease control agent according to claim 4, wherein the plant disease is blast, rust and powdery mildew.