KR20020064747A - Poroligenous liquor acting as a disinfectant vibrio vulnificus and its manufacturing process - Google Patents

Abstract

PURPOSE: A pyroligneous liquid and a manufacturing method thereof are provided to utilize the pyroligneous liquid manufactured through a series of refinement process to sterilize vibrio vulnificus contained in fish and shellfish. CONSTITUTION: An undiluted or diluted solution of the pyroligneous liquid capable of sterilizing vibrio vulnificus is directly sprayed to fish and shellfish, or to sterilize a knife and a chopping board. The manufacturing method of the pyroligneous liquid is comprised of steps of; extracting a basic pyroligneous liquid as a middle layer among three layers produced by stationing certain pyroligneous liquid collected from charcoal smoke more than six months; dehydrating the basic pyroligneous liquid; obtaining a smog perfume pyroligneous liquid by removing tar; and obtaining refined colorless pyroligneous liquid.

Description

Wood liquor used for sterilization of Vibrio bullicus bacteria and its manufacturing method {Poroligenous liquor acting as a disinfectant vibrio vulnificus and its manufacturing process}

The present invention relates to a wood vinegar used for the purpose of preventing vibrio sepsis and a method for manufacturing the same, and more particularly, to remove the wood vinegar obtained as a by-product in the process of manufacturing charcoal by a specific method to remove substances harmful to the human body It relates to a purified wood vinegar solution for sterilizing Vibrio vulnificus derived from seawater and seawater, and a method for producing wood vinegar having the above-mentioned use.

Wood vinegar is produced by capturing and cooling and condensing the smoke generated when carbonizing wood. It contains many beneficial ingredients such as benzoic acid and trace minerals and harmful ingredients such as methanol and phenols. Therefore, in order to use for food, these harmful components must be removed through the purification process.

When the wood vinegar is left for 6 months or more, it is separated into the upper layer, the middle layer, and the lower layer, and the middle vinegar solution is separated from the middle layer. Currently, the purified wood vinegar is used for various purposes. The remaining upper and lower layers are used for soil disinfectants, disinfectants, deodorants, dyeing aids and paints for agricultural and industrial purposes.

Purified wood vinegar is used as a skin disease, such as itching, insect bites, athlete's foot, hair loss, bath and skin-related products, in particular, it is widely used in medicinal use in the private sector due to the efficacy of sterilization, antibacterial, antioxidant, and the like.

It is an object of the present invention to find that the vinegar solution is excellent in the bactericidal action against Vibrio bulitipius bacillus, which causes vibrio sepsis causing serious problems in aquatic product consumption such as summer sashimi, and uses a series of purification processes to use it for food use. It is to provide a wood vinegar used as a fungicide of Vibrio bulitipius bacteria prepared through the method and a method of manufacturing the same.

Method for producing wood vinegar used for sterilization of Vibrio bulnipius bacterium of the present invention, containing the crude wood vinegar obtained in the process of making charcoal in a barrel and allowed to stand for at least 6 months to separate into three layers and to separate only the middle layer A first step of extracting the basal wood vinegar, a second step of heating 1 liter (l) of the basal wood vinegar at normal pressure to about 100 ° C. to evaporate about 500 milliliters (ml), and the remaining 500 ml under reduced pressure at about 80 ° C. Distilling to remove the dissolved tar to obtain a smog-flavored wood vinegar having a brown smog scent of about 300 ml, and 3 w / v% of powdered activated carbon (20-60 mesh) to the smog-flavored wood vinegar; It is characterized by consisting of a fourth step of removing color and smog odor and obtaining a colorless transparent purified wood vinegar.

Smog-flavored wood vinegar and purified wood vinegar prepared from the above process are wood vinegar which is used as a fungicide of Vibrio bulitipicus of the present invention. This wood vinegar can be used as a stock solution or diluted with a 1 ~ 2% solution, which can be directly sprayed on aquatic products such as fish and shellfish, or used to disinfect tools such as cutting boards and knives for handling fish and shellfish.

Comparing the physicochemical properties of the smog-flavored wood vinegar and purified wood vinegar obtained from the above process with those of the basic wood vinegar, the contents of organic acids of Table 2 and phenol and benzopyrene of Table 3 were analyzed using high performance liquid chromatography. Performance Liquid Chromatography (HPLC) or Food and Drug Administration Notice No. 2000-32.

Table 1. Physical Properties of Wood Vinegar

pH Brownness (OD ₄₂₀ ) Basic wood vinegar 2.20 5.09 Smog Scented Wood Vinegar 2.37 0.11 Purified wood vinegar 2.37 0.01

Table 2. Main Organic Acid Contents of Wood Vinegar

Major organic acid (g / ℓ) Acetic acid Propionic acid Lactic acid Isolak Basic wood vinegar 28.91 2.11 0.56 2.50 Smog Scented Wood Vinegar 24.10 1.23 0.18 0.17 Purified wood vinegar 23.95 1.11 0.15 0.08

Table 3. Major Hazardous Substance Contents in Wood Vinegar

Phenol (ppm) Benzopyrene (ppb) Methanol (%) Basic wood vinegar 3688 9.71 1.82 Smog Scented Wood Vinegar 136 3.34 0.00065 Purified wood vinegar 127 3.21 0.00051

In addition, it has the characteristics of the antimicrobial activity as shown in Table 4 together with the physical and chemical properties as described above. In other words, 20-40 μl of purified wood vinegar was applied to 8mm diameter paper disks, dried, and placed on a flat plate coated with some food hygiene microorganisms for 48 hours to examine the antibacterial activity. Table 4 below.

Table 4. Antimicrobial Activities of Smog-flavored and Purified Wood Vinegars against Some Food Sanitary Microorganisms

Throughput (μl) 20 40 E. coli 19/18 * 19.8 / 19.3 Peudomonas aeruginosa 17.5 / 17.4 18.5 / 18.5 Salmonella typhymurium 20 / 19.1 23 / 22.4 Vibrio vulnificus 9.8 / 9.5 17.4 / 16.4 Bacillus cereus 14.5 / 14.7 15.3 / 15.1 Staphylococcus aureues 8.6 / 8.5 11 / 10.5

Note) *: Smog flavored wood vinegar / refined wood vinegar

In addition, wood vinegar solution was added to a marine broth medium at a constant concentration and inoculated with Vibrio bullopus bacteria, and then cultured for 48 hours at 30 ° C., the degree of bacterial growth was absorbed at 600 nm wavelength (Optical Density: OD). The results expressed in values are shown in Table 5.

Table 5. Vibration of Vibrio vulnificus with Purified Wood Vinegar Concentration

Smog-flavored wood vinegar or refined wood vinegar concentration (v / v%) Absorbance (OD ₆₀₀ ) Immediately after inoculation After 48 hours of incubation 0 0.16 / 0.16 * 0.42 / 0.42 0.01 0.16 / 0.16 0.38 / 0.39 0.05 0.16 / 0.16 0.39 / 0.39 0.1 0.16 / 0.16 0.35 / 0.38 0.5 0.16 / 0.16 0.33 / 0.37 One 0.16 / 0.16 0.06 / 0.06 2 0.16 / 0.16 0.05 / 0.05

Note) *: Smog flavored wood vinegar / refined wood vinegar

In particular, the wood vinegar of the present invention has an excellent bactericidal power against Vibrio bulnipius bacteria, the results of the septic bacterium survival rate according to the contact time with smog-flavored wood vinegar and purified wood vinegar in marine brine, the growth medium of septic bacteria Same as 6. Survival was performed by plate smearing.

Table 6. Survival Rate of Sepsis Bacteria by Contact Time with Purified Wood Vinegar

badge Contact time (min) Survival rate (%) Smog-flavored wood vinegar or refined wood vinegar concentration (v / v%) 0 0.5 One 2 sea water 0 100/100 * 100/100 100/100 100/100 One 100/100 89/90 72/79 14/18 5 100/100 46/52 35/38 0.1 / 0.3 10 100/100 44/47 7/10 0 / 0.1 15 100/100 43/46 4.5 / 7.2 0/0 20 110/110 35/39 2.9 / 4.2 0/0 30 118/118 24/25 0 / 0.7 0/0 60 136/136 78/83 0/0 0/0 120 145/145 105/109 0/0 0/0 Marine Bruce 0 100/100 nd ** 100/100 100/100 One 100/100 nd ** 89/90 0.4 / 0.6 5 104/104 nd ** 62/64 0.3 / 0.3 10 106/106 nd ** 10/12 0.1 / 0.2 15 106/106 nd ** 3 / 4.5 0.1 / 0.16 20 107/107 nd ** 2.5 / 3.5 0 / 0.01 30 107/107 nd ** 1 / 1.1 0/0 45 107/107 nd ** 0 / 0.8 0/0 240 161/161 nd ** 0/0 - 360 560/560 nd ** 0/0 -

Note) *: Smog flavored wood vinegar / refined wood vinegar; **: no measurement

As described above, the light brown smog-flavored herb and colorless purified herbaceous vinegar of the present invention show excellent bactericidal effect against not only some food sanitary microorganisms but also Vibrio bulnipicus bacteria, leading to disinfectant disinfectants or food products in aquatic product processing houses or homes. Can be used as a fat and oil. Particularly, when making sashimi, etc., it is possible to use it as a material to improve the texture of sashimi and to prevent sepsis caused by Vibrio bulnipicus bacteria, as well as maintaining the freshness of sashimi by diluting with a proper concentration and spraying or smearing.

As described above, the present invention removes the harmful substances contained in the wood vinegar through a specific purification process, thereby making it possible to utilize the functionality of the wood vinegar for food use. In particular, wood vinegar showed strong bactericidal activity against Vibrio blnipicus bacteria, a causative agent of sepsis, which is a problem in summer seafood consumption.Because spraying or smearing smog-flavored wood vinegar or refined wood vinegar to shellfish or other utensils for treating them can kill sepsis It is very convenient to prevent vibrio sepsis.

Claims (5)

Used to sterilize Vibrio bulnipicus or sterilize Vibrio bulnipicus by spraying or smearing stock solution or diluent directly onto fish or shellfish with sterilizing effect. Wood vinegar. The first step of extracting the basic wood vinegar for purification of wood vinegar by removing the crude wood vinegar obtained from the process of making charcoal in a barrel and left for three months and separated into three layers and separating only the middle layer. A second step, a third step of removing tar dissolved in the basal wood vinegar to obtain a smog-flavored wood vinegar, and a fourth step of removing color and aroma of the smog-flavored wood vinegar to obtain a colorless transparent purified wood vinegar A method for producing wood vinegar, used for sterilization of Vibrio bulitipicus. The method of claim 2, wherein the second step of removing water is Vibrio bulitipius bacteria, characterized in that by evaporating to a volume of about half of the predetermined volume by heating a predetermined volume of the basic wood vinegar solution at about 100 ℃ at atmospheric pressure Method of preparing wood vinegar for use in sterilization. The method of claim 2, wherein the third step of removing the tar to obtain a smog-flavored wood vinegar is distillation under reduced pressure to remove the dissolved tar at a volume of about half the remaining volume at about 80 ℃ after evaporation to about 60% of the volume Method of producing wood vinegar used for the sterilization of Vibrio bulitipius bacteria, characterized in that to obtain a wood vinegar having a brown smog flavor. The fourth step of removing color and aroma of the smog-flavored wood vinegar to obtain a colorless transparent purified wood vinegar is to add 3w / v% of powdered activated carbon (20-60 mesh) to the crude wood vinegar, stirred for 30 minutes and filtered Method of producing wood vinegar used for the sterilization of Vibrio bulitipius bacteria, characterized in that to remove the smell of smog and obtain a colorless transparent purified wood vinegar.