KR20020064088A - Light ripening of drug·food - Google Patents

Abstract

PURPOSE: A method for treating crude drug and food by irradiating far infrared rays with a ceramic body to the crude drug and food is provided. Whereby, antioxidant function and anticancer function of the crude drug and food selected from loess, Amydae Carapax, Psoraleae semen, Biota orientalis, Artemisiae asiaticae Herba, Eugenia caryophyllus, walnut skin, Acanthopanax Senticosus or the like are increased. CONSTITUTION: Loess is soaked in water, mixed with 20 to 30% by weight of gypsum while heating at 90 to 105deg.C, based on the weight of the loess, mixed with Fossilia Ossis Mastodi and talc fewer than the amount of the gypsum, and cooled to form a coagulum, which is ground, mixed with 5 to 10% by weight of rosin and edible glue no fewer than the amount of the rosin and calcined to a rough molded product. Actinolite is steeped in water at 95 to 115deg.C for 2 to 3hr and the rough molded product is soaked therein and dried to produce a ceramic body that is used for treatment of crude drug and food.

Description

Light ripening method of medicine, food {LIGHT RIPENING OF DRUG, FOOD}

The present invention relates to a method of treating medicinal herbs and / or foods using far infrared rays (hereinafter only referred to as the 'photoaging method'). More specifically, the present invention relates to a photoaging method of increasing anticancer properties of medicines and / or foods by irradiating far-infrared rays to medicines and / or foods to be treated through a specific ceramic body.

Cancer is one of the biggest problems to be solved in human civilization, and much research has been done to treat cancer. Cancer treatments include radiotherapy, gene therapy, and the like, but these treatments are effective when diagnosed and detected early, but do not show satisfactory effects when the development of cancer is somewhat advanced.

In recent years, there is a growing interest in developing anticancer therapies for treating cancer using herbal medicines known to have anticancer efficacy.

On the other hand, as a chemotherapy method, many methods using far-infrared rays which are useful to a human body have been researched.

Photo-aging means the ripening through light energy, and the principle is the resonance principle using a specific wavelength. For example, in the medical field, infrared radiation is irradiated to the human body to promote inflammation treatment and blood flow improving effect.

However, no method has yet been developed for the photosynthesis of anticancer drugs and / or anticancer functional foods using far infrared rays.

Accordingly, an object of the present invention is to provide a photomaturation method that can increase the anticancer effect of medicines and / or foods using far infrared rays.

1 is a schematic view showing a light aging method according to the present invention.

Figure 2a is a graph showing the measurement of free radicals of general cancer patients, Figure 2b is a graph showing the measurement results of the active oxygen of cancer patients measured after 2 hours taking Gugikgigitang, Figure 2c is a photoaging method according to the present invention It is a graph showing the results of measurement of active oxygen of the patient after taking 2 hours after taking the ripened gukgigitang.

Figure 3 is a graph showing the anticancer effect of the medicine prepared by the present invention.

According to the invention,

Immersing the red paper to form wet red paper;

Mixing 20-30% by weight of gypsum based on the weight of the red coal paper while heating the wet red coal paper at 90-105 ° C., and then adding a smaller amount of keel and talc to the mixture to cool the mixture to form a coagulum. step;

Pulverizing the coagulated product, adding and mixing 5-10 wt% of rosin and an amount of edible glue less than the rosin based on the weight of the coagulated product, and then calcining to form a primary molded product;

Preparing a ceramic body by immersing Yang Ki-Suk in the water of 95-115 ° C. for 2 to 3 hours, and then immersing the primitive molded product in the water soaked. And

Placing a medicine and / or food to be treated under the ceramic body and irradiating far infrared rays to the medicine and / or food from the ceramic body through the ceramic body;

There is provided a photosynthesis method comprising a.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.

It relates to the photoaging of medicines and / or foods using far-infrared rays, wherein the anti-cancer efficacy of the treated medicines and / or foods is increased by irradiating far-infrared rays through a ceramic body specific to the anti-cancer medicines and / or foods to be treated. Was found.

In order to give anti-cancer efficacy to medicines and / or foods to be treated, ceramics through which far-infrared light passes through far-infrared irradiation are manufactured by treating, mixing, and grinding red paper, gypsum, keel, talc, rosin, and edible glue, respectively.

First, wet red paper is formed by soaking red paper. Redwood paper has a neutralizing function and stabilizes the human digestive system. The reason for immersing the red paper is to mix well with the next gypsum added. Preferably, wet red paper having a water content of 200 to 300% is prepared by soaking the red paper for 2-3 days. Allow the sedimentary soil to soak for at least two days to have sufficient moisture content. However, more than three days of soaking water content increases gypsum combination is not good. It is also difficult to react with gypsum at less than 200% water content, and inhibits the reaction with talc added at water content above 300%.

20-30% by weight of gypsum is added and mixed based on the weight of the red paper while the wet red paper is heated to 90 to 105 ° C. The reason for heating the wet red paper is that the red paper and the gypsum are best mixed when the moisture of the red paper is almost dried under the above heating conditions. In addition, when the amount of gypsum added is less than 20% by weight or 30% by weight or more based on the weight of red coal paper, it is difficult to mix with the keel to be added later. Preferably 20 to 25% by weight of gypsum is added based on the weight of red paper. The gypsum is preferably Hubei gypsum.

Then, when the mixture begins to solidify, a smaller amount of keel and talc is added to the mixture than the gypsum, followed by cooling to form a solid. Preferably, the mixture is added by adding 1.5 to 2% by weight of keel and 1.5 to 2% by weight of talc based on the weight of red coal paper.

The keel acts to stabilize the red tide, and the talc doubles the neutralization function of the red tide. At this time, it is preferable to match the addition amount of keel and talc equally. The amount of the keel and the talc should be the same, so that it reacts well with the red tide and solidifies. If one of them is large, there is a problem of caramelization without solidification.

After the coagulum is pulverized into powder, 5-10% by weight of rosin and an amount of edible glue less than the rosin are added and mixed, and then calcined to form a primary molded product. Rosin enhances the cleansing function of the powder, and food glue stabilizes the urinary system and maintains the efficacy of red paper. When the addition amount of the rosin is added in less than 5% by weight or 10% by weight or more based on the weight of the coagulant, complete curing of the powder is not achieved. In addition, rosin is hardened when the amount of food glue added is larger than the rosin. Preferably it is added 5 to 6% by weight rosin and edible glue 7 to 8% by weight based on the weight of the coagulum. Preferred firing conditions for forming the primary molded product are 5 to 6 hours at 280 to 300 ° C.

Thereafter, the primitive molded product is immersed in water in which quantitative stone is soaked, and then dried to prepare a ceramic body. Yanggi stone penetrates into the red rock and increases the weakness of the red rock (the red and yellow rocks are the best in wavelength resonance when MRA wave test results). Here, the water brewed with the positron is prepared by brewing the mutant with 95-115 ° C. for at least 2 hours. The temperature of distilled water is best at the temperature of the above range when brewing the positron, and if the time of brewing the quantitative stone is less than 2 hours, the positron component does not come out well. Preferably, the time to boil the fern is 2-3 hours.

When the molded product is submerged, it is preferable to submerge it at a water content of 25 to 30%.

Subsequently, the photo-maturated ceramic body is placed at a distance of 25-30 cm under the infrared light, and the lower portion of the photo-maturation is placed by placing a medicine and / or food to be treated to impart anticancer and anti-oxidant efficacy. Permeate ceramic powder to be irradiated to the medicine and / or food. When the distance between the photoaging ceramic powder and the far infrared ray is closer than 25 cm, the medicine and / or food to be matured is denatured by the high temperature of the far infrared ray, and the wavelength ripening is weak when it is 30 cm or more. A far infrared ray lamp of 250 W is preferable.

The ceramic body may be pulverized to produce a ceramic powder. When the ceramic body or ceramic powder is disposed under far infrared rays, an appropriate thickness is 2 to 3 mm.

1 is a schematic view showing a photo-aging treatment method of medicines and / or foods according to the present invention.

As shown in FIG. 1, far infrared rays may be irradiated to medicines and / or foods to be treated through ceramic powders on top of the photo-aging ceramic powders.

The photo-aging ceramic powder irradiated with far infrared rays emits its own wavelength and is transmitted to the medicine and / or food to be treated below. Far infrared rays are suitable for induction of cell resonance in the body, and halogens and other heats may inhibit the maturity of the redwood powder.

Herbs that are provided with anticancer function by photosynthesis of the present invention is not limited to this, but tortoiseshell, pagoji, sesame, baekyeop, love leaf, cloves, hodopi, hongsongjin, bokyeong, donkey, perforation, peony, licorice, horse riding Contains seahawks, thorns, and red sea ginseng.

In addition, the method according to the invention can be applied to the production of anti-cancer functional food by applying to any food.

Hereinafter, the present invention will be described in detail through examples.

Example 1 Preparation of Ceramic Powder for Photoaging

300 g of red stone paper (赤石脂, a kind of herbal medicine) was soaked in water for 70 hours to prepare a wet red paper of about 200% moisture content. Then, the wet red coal paper was heated at 100 ° C. within 20 minutes, and then mixed with 60 g of Hubei gypsum, and after 10 minutes, when the red paper and gypsum were solidified, 4.5 g of keel and 4.5 g of talc were added and mixed. After the heating to 56 ~ 60 ℃ temperature it was cooled and solidified at room temperature to prepare a coagulum.

The coagulum was pulverized to a powder, and 20 g of rosin and 20 g of edible glue were added thereto, mixed, and kneaded so that the water content was 20-30% using lead snow water, which is snow melted in December. After that, it was molded into bricks, and then baked for 6 hours in a ceramic oven at 300 ° C. for primary molding.

Subsequently, the brick-shaped molded product prepared as described above was immersed for 5 hours in water having boiled yang seok stone at 100 ° C. for 130 minutes to have a water content of about 30%. Then dried at room temperature and ground to prepare a ceramic powder.

Example 2: Photoaging

As shown in Fig. 1, the ceramic powder prepared in Example 1 was laminated on the container in the thickness of the following Table 1, and the far-infrared light (Philips 250W) on the top of the herbal medicine to be treated through the ceramic powder Irradiation was made for 1 hour.

Herbal medicine mixture is 3% by weight of Tortoiseshell, 3% by weight of pagoji, 5% by weight of white leaf, 5% by weight of young leaves, 0.5% by weight of clove, 1.5% by weight of larvae, 15% by weight of red pine ginseng, 2% by weight of red ginseng, 2% by weight of Fuling, 15% by weight, Angelica 3% by weight, 35% by weight of oak, 2% by weight of peony, 1% by weight of licorice, 1.5% by weight of horse riding, 0.5% by weight of sea tiger, and 5% by weight of red sea ginseng.

Example 3: By Magnetic Resonance Analyzer (MRA) One anticancer measure

Comparison of Inventive Examples 1-3, Control and Comparison with Magnetic Resonance Analysis (Plant, Resonance Number, Higher Resonance, Higher Anticancer Effect. Presented by Korean Wave Life Scholarship 99. 7) For example, by measuring the resonance for the malignant tumor and immune function of the treated medicine is shown in Table 1 below.

The ceramic powder used in Inventive Examples 1-3 and the medicines to be treated are as described in Examples 1 and 2 above. However, the lamination thickness and the far-infrared irradiation time of the ceramic powder were different as described in Table 1 below.

As a control, a general herbal medicine (6 years old Jeonggwanjang ginseng extract, which has been found to have anticancer efficacy) was used, which was not irradiated with far infrared rays, and as a comparative example, except that irradiated with far infrared rays for 6 hours without using the ceramic powder. Was used as a herbal medicine prepared in Example 1.

Control Inventive Example 1 Inventive Example 2 Inventive Example 3 Comparative example Treatment method General Herbal Medicine Powder thickness 2mm far infrared rays-6 hours irradiation Powder thickness 2mm far infrared rays-7 hours irradiation Powder thickness 4mm far infrared rays-6 hours irradiation Ceramic powder not used Far-infrared radiation for 6 hours Malignant tumor ¹ (F005) +9 +21 +20 +19 +11 Immune function ² (B222) +6 +21 +19 +16 +7

1: Malignant tumor is a characteristic signal common to malignant tumors in each region.

2: Immune function is an interference signal that can offset the wavelength of malignant tumor, which means the therapeutic resonance wavelength in human body.

Reference:

The number of malignant tumors and immune functions refers to the MRA recognition number.

The more powerful the anticancer agent, the higher the F005 malignancy is. Ex) Taxol +20, distilled water 0, Beijing Chuo University Cancer Cure

Example 4: evaluation of improvement of active oxygen removal ability

This example shows that the active oxygen, a harmful oxygen in the body, is significantly reduced by taking the photo-aged herbs of the present invention.

Blood was collected from a patient (leukemia 19-year-old female Lee Hyun-hyeon) to measure the free radicals. Free radicals were measured using a free radical meter (BJL Ultra-weak Chemiluminescence Analyzer, manufactured by BJL, Bradford Laboratories, USA).

After taking 50g of the lactose solution consisting of 50% by weight of sesame, 25% by weight of pine rosin and 25% by weight of red sea ginseng to the patient, 1 혈액 of blood was collected from the patient and the active oxygen was measured in the same manner as described above.

On the other hand, the 50% of the lacquer, 25% Yujin pine, 25% red sea ginseng powder 25% of the lyotropic liquid using the photoaging powder of Example 1 irradiated with far-infrared radiation for 6 hours to 2mm thickness, and then 50g once to the patient After taking 2 days / day, blood was collected and free radicals were measured in the same manner as above. The results are shown in FIG.

As shown in FIG. 2, it can be seen that when the patient is administered Gugikgi-tang, which is matured by the photoaging method of the present invention, free radicals, which are harmful oxygen in the body, are significantly reduced.

Example 5: Comparison of the efficacy of ACM700 with cancer treatment ACM700 and ACM900 added with a photo-aging drug.

As shown in Table 2, ACM700 (25% by weight of milk rosin, 50% by weight of lacquer lactose and 25% by weight of red sea ginseng herbal medicine) used as a comparative drug for 133 cancer patients (Gwanghyewon Oriental Medical Hospital) The prescription drug) and ACM900 (anticancer agent treated with photosynthesis according to the present invention, ACM900) used as the invention example were taken to 133 cancer patients, and survival rate was measured after 1 year, and is shown in FIG. 3.

As shown in FIG. 3, when the photo-aging drug (ACM900) was administered by the photoaging method according to the present invention, the survival rate of patients was the highest at 76%. This survival rate is higher than that of Kwang Hye Won Oriental Medicine Hospital (ACM700, 53% survival rate).

Example 6 Performance Evaluation of Photosaturated Anticancer Agents

The anticancer activity of the medicinal ACM900 of Example 5 against various cancer cells was examined by SRB method (Proc. Am. Assoc. Cancer Res., 30: 612, 1989, Skehan et al.).

As cancer cells, breast cancer cell line MCF7, skin cancer cell line M14, LOX-IMVI, central nervous system (CNV) related cancer cell line SNB19, colon cancer cell line HCT116, COLO205, renal cancer cell line UO-31, lung cancer cell line NCI- H226, A549 was used. The cancer cell line was cultured using RPMI 1640 medium containing 10% fetal bovine serum, and the cultured cells were maintained by dispensing once or twice a week. The cell concentration used to measure anticancer activity was 3,000-6,000 cells / ml. All the reagents used were dissolved in 100% dimethylsulfoxide (DMSO) and diluted in stages to increase the concentration of the reagents. 0.3 and 0.1 µg / ml.

After measuring the number of cells and dispensing them in 96-well plates at a constant concentration, and after one day, the Tz (Time / Zero) plate required to determine the basic absorbance of the cells, having the same cell concentration as the plate to be treated. The other plate was fixed using 50% TCA (Trichloroacetic Acid, fixed solution). The plate to be treated with the sample was treated with five sample concentrations by adjusting the final concentration of the sample to 0.1% DMSO. Tz plate was washed with tap water after 1 hour, and the plate treated with sample was fixed with 50 μl of 50% TCA per well after 2 days, and also with tap water after 1 hour. It was. The washed plate was dried at room temperature, and then 100 μl of 0.4% SRB solution (solution dissolved in 1% acetic acid) was added per well, and then washed with 1% acetic acid solution after 30 minutes and dried again at room temperature.

Then, 10 μl tris base (pH 10.5) was added to 10 μl per well to dissolve again, and the absorbance was measured at 540 nm using an ELISA reader by an enzyme-linked immunosorbent assay (ELISA). In this case, GI 50 (μg / ml) represents the concentration of the compound that inhibits the growth of cancer cells by 50%. Comparison was made using adriamycin (10-0.1 µg / ml) as a comparative group. Compared with adriamycin (an existing potent anticancer agent), the results were comparable, and the natural product showed great efficacy.

The results are shown in Table 3 below.

Cancer cell line Cancer cell growth inhibitory effect (GI ₅₀ (㎍ / ml)) A549 2.02 NCI-H226 4.53 UO-31 1.06 Colo205 2.51 HCT116 2.19 SNB19 5.19 LOX-IMVI 3.43 M14 1.29 MCF7 2.17

As shown in Table 3, it was confirmed that the photosynthetic medicinal herbs ACM900 of the present invention showed a strong activity of inhibiting growth against nine cancer cells.

The anti-aging and anti-cancer functions of the medicinal herbs and / or foods are increased by photoaging the medicinal herbs and / or foods using the far-infrared rays and the ceramic powder of the present invention.

Claims (14)

Immersing the red paper to wet the red paper; Mixing 20-30% by weight of gypsum on the basis of the weight of the red coal paper while heating the wet red coal paper to 90 ~ 105 ℃ and then adding and mixing a smaller amount of keel and talc than the gypsum in the mixture to cool to form a coagulum step; Pulverizing the coagulated product, adding and mixing 5-10 wt% of rosin and an amount of edible glue less than the rosin based on the weight of the coagulated product, and then firing to form a primary molded product; Preparing a ceramic body by immersing Yang Ki-Suk in the water of 95-115 ° C. for 2 to 3 hours, and then immersing the primitive molded product in the water soaked. And Placing a medicine and / or food to be treated under the ceramic body and irradiating far infrared rays to the medicine and / or food from the ceramic body through the ceramic body; Photosaturation method comprising a. The method of claim 1, further comprising, after manufacturing the ceramic body, pulverizing the ceramic to form a ceramic powder. The method of claim 1, wherein the medicinal herbs and / or foods are tortoiseshell, pagoji, baekryeop, larvae, cloves, hawthorn, prickly pear, red ginseng, bokyeong, donkey, perforated, peony, licorice, sesame, horseback riding, shiho, big pine gin and red sea ginseng Selected from minutes. The method of claim 1, wherein the water content of the wet calcined paper is 200 to 300%. The method of claim 1, wherein the mixing amount of gypsum is 20 to 25% by weight based on the weight of red paper. The method of claim 1, wherein the mixing amount of the keel is 1.5 to 2% by weight based on the weight of the red paper. The method of claim 1, wherein the mixed amount of talc is 1.5 to 2% by weight based on the weight of the red paper. The method of claim 1, wherein the mixing amount of the rosin is 5 to 6% by weight based on the weight of the coagulant. The method of claim 1, wherein the mixing amount of the edible glue is 7 to 8% by weight based on the weight of the coagulant. The method of claim 1, wherein the firing conditions for forming the primary molding are 5 to 6 hours at 280 to 300 ℃. The method according to claim 1, wherein the water content is 25 to 30% when the priming molding is immersed in the water brewing ferrite. The method of claim 1, wherein when the ceramic body is disposed, the thickness of the ceramic body is 2 to 3 mm. The method according to claim 1, wherein when disposing the ceramic body, the distance from far infrared rays is 25 to 30 cm. The method of claim 1, wherein the ceramic body comprises a powder form.