KR20020061247A - Process for treating organic wastewater by using yeast - Google Patents

Process for treating organic wastewater by using yeast Download PDF

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KR20020061247A
KR20020061247A KR1020010002197A KR20010002197A KR20020061247A KR 20020061247 A KR20020061247 A KR 20020061247A KR 1020010002197 A KR1020010002197 A KR 1020010002197A KR 20010002197 A KR20010002197 A KR 20010002197A KR 20020061247 A KR20020061247 A KR 20020061247A
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yeast
organic
organic acid
wastewater
acid
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KR1020010002197A
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Korean (ko)
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김은기
김순영
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자연엔지니어링 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/347—Use of yeasts or fungi
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F7/00—Fertilisers from waste water, sewage sludge, sea slime, ooze or similar masses
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Water Supply & Treatment (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE: Provided is a method for treating organic wastewater using yeast which uses organic acid as carbon source. CONSTITUTION: Yeast is strong at low pH, and settled better than bacteria. According to the method, the method comprises two steps: (a) step for decomposing organics into organic acid; and (b) injecting yeast into wastewater, contained organic acid, for decomposing organic acid. Yeast used for treating wastewater is recycled as feed.

Description

효모를 이용한 유기 폐수의 처리방법{Process for treating organic wastewater by using yeast}Process for treating organic wastewater by using yeast

본 발명은 유기 폐수를 처리하는 방법에 관한 것이다. 더욱 상세하게는, 고농도의 유기물 폐수를 유기산으로 전환시키고 이를 효모로 분해시킨 후 효모를 회수하는 것을 특징으로 하는, 유기물 폐수의 처리방법에 관한 것이다.The present invention relates to a method of treating organic wastewater. More specifically, the present invention relates to a method for treating organic wastewater, which comprises recovering yeast after converting the high concentration of organic wastewater into an organic acid and decomposing it into yeast.

고농도의 유기물 폐수는 식품 공장 등에서 주로 발생되는 폐수로서 생물학적 산소 요구량(BOD)이 상당히 높고(3,000 - 20,000 ppm), 비교적 미생물에 의해 분해가 잘 되는 유기폐수를 말한다.High concentration organic wastewater is a wastewater mainly generated in food factories, etc., and refers to organic wastewater which has a high biological oxygen demand (BOD) (3,000 to 20,000 ppm) and is relatively decomposed by microorganisms.

이를 처리하기 위한 종래의 기술은 공업용수 등으로 희석하여 활성 오니법을 사용하는 것이었으나, 이 방법은 처리 용량이 증가함에 따라 막대한 시설 비용을필요로 한다.The conventional technique for treating this was to use an activated sludge method by diluting with industrial water or the like, but this method requires enormous facility costs as the treatment capacity increases.

다른 방법으로서, 먼저 혐기성 미생물로 전처리를 하여 BOD를 낮춘 다음, 2차로 활성 왜법을 사용하는 방법이 있다.Another method is to first pretreat with anaerobic microorganisms to lower BOD, and then use active anamorphism second.

통상의 혐기성 처리는 유기산 생성단계와 유기산이 메탄으로 생성되는 메탄생성 단계로 크게 구분되며, 두 단계의 반응이 보통 한 탱크에서 일어나지만, 이를 두 단계로 분리하여 운전하는 경우도 있다. [Massey, M.L., F.G. Pohlard (1978) Phase Separation of anaerobic stabilization by kinetic control Research, J.W.P.C.F.13, 2205-2209]Conventional anaerobic treatment is largely divided into organic acid generation step and methane production step in which organic acid is generated as methane. Two-step reactions usually occur in one tank, but are sometimes operated in two stages. Massey, M.L., F.G. Pohlard (1978) Phase Separation of anaerobic stabilization by kinetic control Research, J.W.P.C.F. 13, 2205-2209].

그러나, 혐기성 미생물로 전처리를 하는 경우, 체류 시간이 길어지고 시설이 커지며 운전 및 유지가 어려운 단점이 있다. 뿐만 아니라, 혐기성 과정은 유기물이 유기산으로 전환되고 이것이 다시 메탄으로 전환되는 두 단계로 크게 구분되는데, 특히 두 번째 단계에서 메탄 생성균의 성장 조건이 까다로우므로 전체 혐기성 처리를 곤란하게 한다. 더욱이, 일단 혐기성 공정이 시작되어도 외부 pH나 중금속 등에 의해 많은 영향을 받는 등의 어려움이 있다.However, in the case of pretreatment with anaerobic microorganisms, the residence time is long, the facility is large, and operation and maintenance are difficult. In addition, the anaerobic process is largely divided into two stages, in which organic matter is converted to organic acid, which is then converted back to methane. Particularly, in the second stage, the growth conditions of methane-producing bacteria are difficult, which makes the whole anaerobic treatment difficult. Moreover, even once the anaerobic process is started, there are difficulties such as being greatly influenced by external pH or heavy metals.

따라서, 본 발명자들은 이러한 종래 문제점을 해결할 수 있는 효과적인 유기물 폐수 처리방법에 관한 연구를 거듭한 결과, 고농도의 유기물 폐수를 유기산으로 전환시키고, 이를 효모를 이용하여 분해시킨 다음, 증식된 효모를 회수하는 방법에 의해 이러한 문제점이 해결될 수 있음에 착안하여 본 발명을 완성하였다.Therefore, the present inventors have conducted a study on an effective organic wastewater treatment method that can solve such a conventional problem, converting the high concentration organic wastewater into an organic acid, decomposing it using yeast, and then recovering the grown yeast The present invention has been completed by focusing on the fact that this problem can be solved by the method.

도 1은 본 발명에 따른 유기 폐수의 처리공정을 간략하게 나타낸 도면이다.1 is a view briefly showing a process for treating organic wastewater according to the present invention.

본 발명은, 유기물을 유기산으로 전환시키는 단계(1단계)와 상기 유기산을 탄소원으로 하는 효모를 폐수에 첨가하여 배양함으로써 유기산을 분해하는 단계(2단계)를 포함하여 이루어 진다.The present invention comprises a step (step 1) of converting an organic material into an organic acid and a step (step 2) of degrading the organic acid by culturing by adding the organic acid as a carbon source to yeast.

상기 언급된 통상의 혐기성 처리방법에서와 같이, 1단계는 주로 유기산 생성 박테리아에 의해 진행이 되고, 생성되는 유기산은 주로 초산, 젖산, 뷰티릭산, 프로페놀산 등이다. 이 단계는 빠른 속도로 진행되어 보통 5-6 시간 내에 완결되는 반응이며, 또한 공기가 공급되지 않는 반응이므로 에너지가 크게 소요되지 않는다. 1단계에서 생성된 유기산은 pH를 3-4로 낮추게 된다.As in the conventional anaerobic treatment methods mentioned above, step 1 is mainly carried out by organic acid producing bacteria, and the organic acids produced are mainly acetic acid, lactic acid, butyric acid, propenolic acid and the like. This step is fast and usually completes in 5-6 hours, and it does not require much energy because it is a reaction without air. The organic acid produced in step 1 lowers the pH to 3-4.

통상적인 혐기성 처리의 2단계는 pH 3-4의 낮은 pH를 다시 중화시킨 다음 메탄을 생성했으나, 본 발명에서는 2단계에서 유기산을 중화할 필요가 없이 효모로 직접 처리하는 방법을 선택하였다. 유기산은 효모에게 좋은 탄소원이 될 수 있으며, 유기산을 탄소원으로 하는 효모를 배양하면 효모는 낮은 pH에서도 성장이 가능할 뿐 아니라, 박테리아보다 쉽게 침전될 수 있고, 잉여로 생산된 효모는 회수하여 사료 등으로 이용할 수 있다. 유기산이 분해된 폐수는 pH를 쉽게 7 부근으로 원위치시킬 수 있으므로, 방류에 문제가 없다. 본 발명의 공정의 흐름은 도 1에 나타내었다.The second stage of the conventional anaerobic treatment neutralizes the low pH of pH 3-4 again to produce methane, but the present invention chose the method of treating directly with yeast in the second stage without the need to neutralize the organic acid. Organic acids can be a good carbon source for yeast, and if you cultivate yeasts using organic acids as a carbon source, yeast can grow at low pH, can be more easily precipitated than bacteria, and excess yeast can be recovered and used as feed. It is available. Wastewater from which the organic acid is decomposed can easily return the pH to around 7, so there is no problem of discharge. The flow of the process of the present invention is shown in FIG.

상기에서 사용가능한 효모의 종류는 유기산을 탄소원으로 하는 효모로서 독성이 없는 것이면 특별한 제한없이 사용될 수 있으며, 예를 들면 쉬조사카로마이세스 폼페(Schizosaccharomyces pombe) 9987 등이 바람직하게 사용된다.Kinds of yeast that can be used in the above can be used without particular limitation as long as there is no toxicity as a yeast having an organic acid as a carbon source, for example, Schizosaccharomyces pombe 9987 and the like are preferably used.

<실시예><Example>

이하에, 본 발명을 실시예를 통하여 보다 상세히 설명한다. 다만, 본 발명은 이들 실시예에 국한되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to Examples. However, the present invention is not limited to these examples.

실시예 1: 효모에 의한 유기산의 분해Example 1 Degradation of Organic Acid by Yeast

효모(Schizosaccharomyces pombe9987/ ATCC에서 구입)를 YM배지(효모 추출물 3 g/ℓ, 맥아추출물 3 g/ℓ, 펩톤 5 g/ℓ, 포도당 10 g/ℓ) 중 30℃에서 2일간 배양하였다. 유기산이 첨가된 유기산 배지는 유기산 생성조(도 1)에서 생성된 유기산의 종류 및 농도가 유사하도록 조성하였다 (배지 1 리터 중 : K₂HPO₄0.2g, (NH₄)₂SO₄ 0.22g, NH₄HCO₃ 0.65g, Na₂CO₃0.25g, CaCl₂ 0.055g, 효모추출물 0.1g, 뷰티릭산 4g, 초산 3g, 프로피놀산 0.5g).Yeast (purchased from Schizosaccharomyces pombe 9987 / ATCC) was incubated for 2 days at 30 ° C. in YM medium (3 g / l yeast extract, 3 g / l malt extract, 5 g / l peptone, 10 g / l glucose). The organic acid medium to which the organic acid was added was prepared such that the type and concentration of organic acid produced in the organic acid generating tank (FIG. 1) were similar (in 1 liter of medium: K₂HPO₄0.2g, (NH₄) ₂SO₄ 0.22g, NH₄HCO₃ 0.65g, Na₂CO₃0. 25 g, CaCl2 0.055 g, yeast extract 0.1 g, butyric acid 4 g, acetic acid 3 g, propinolic acid 0.5 g).

상기 유기산 배지 100 mℓ를 500 mℓ 들이 플라스크에 넣고 YM배지에서 배양한 배양액을 5%가 되도록 첨가 후, 30℃, 200 rpm에서 7일간 배양하였다.100 ml of the organic acid medium was added to a 500 ml flask, and cultured in a YM medium was added to 5%, followed by incubation at 30 ° C. and 200 rpm for 7 days.

원심분리 후, 100℃에서 균체의 건조중량을 측정하였고, 유기산의 정량은 FID(불꽃이온검출기)를 장착한 가스크로마토그래피를 이용하였다. 총 유기탄소는 TOC 분석기를 사용하여 측정하였다.After centrifugation, the dry weight of the cells was measured at 100 ° C., and the organic acid was quantified by gas chromatography equipped with a FID (flame ion detector). Total organic carbon was measured using a TOC analyzer.

7일 동안의 유기산 분해 결과를 표1에 나타낸다.Table 1 shows the results of organic acid decomposition for 7 days.

배양일(day)Culture day 00 1One 22 33 44 55 66 77 초산(ppm)Acetic acid (ppm) 40004000 30003000 18001800 14001400 800800 300300 100100 2020 뷰티릭산(ppm)Butyric acid (ppm) 30003000 12001200 200200 00 00 00 00 00 프로피온산(ppm)Propionic acid (ppm) 500500 400400 300300 180180 8080 2020 55 00 총유기탄소(ppm)Total Organic Carbon (ppm) 38003800 20002000 12001200 10001000 300300 150150 7070 5050 균체량(g/ℓ)Cell weight (g / ℓ) 00 0.10.1 0.40.4 1.01.0 1.11.1 1.21.2 1.31.3 1.31.3 pHpH 4.94.9 5.25.2 5.75.7 6.36.3 6.56.5 6.86.8 7.57.5 8.08.0

위 결과에서 보는 바와 같이 효모는 각종 유기산을 분해하였으며, 총 유기탄소의 양이 감소하는 것으로 보아서 분해된 유기산이 중간산물로 폐수에 남아있지 않고 완전히 분해됨을 알 수 있다.As can be seen from the above results, yeast decomposed various organic acids, and the total amount of organic carbon decreased, indicating that the decomposed organic acid was completely decomposed without remaining in the wastewater as an intermediate product.

실시예 2: 효모에 의한 고농도 유기폐수의 처리Example 2: Treatment of High Concentration Organic Wastewater with Yeast

식품공장 폐수(pH7, 총 유기탄소 4500 ppm) 5 리터를 1일간 30℃에서 교반기를 이용, 100 rpm으로 교반하고, 5 시간 동안 정치시켰다. 유기물을 유기산으로 전환시키는 박테리아는 폐수 내에 자연적으로 존재하는 것을 이용한다.Five liters of food plant wastewater (pH7, 4500 ppm total organic carbon) was stirred at 100 rpm using a stirrer at 30 ° C. for 1 day and allowed to stand for 5 hours. Bacteria that convert organics to organic acids utilize what is naturally present in the wastewater.

YM 배지 1ℓ에서 배양하고 침전분리시킨 효모 10 g을 상기 상등액 4 ℓ에 첨가하고 5일 간 배양하였다. 결과를 표 2에 나타내었다.10 g of yeast cultured in 1 L of YM medium and precipitated were added to 4 L of the supernatant and incubated for 5 days. The results are shown in Table 2.

배양일(day)Culture day 00 1One 22 33 44 55 66 총유기산(ppm)Total Organic Acid (ppm) 75007500 45004500 21002100 19001900 18001800 16001600 15001500 총유기탄소(ppm)Total Organic Carbon (ppm) 37003700 21002100 15001500 14001400 10001000 900900 800800 균체량(g/ℓ)Cell weight (g / ℓ) 0.050.05 0.430.43 0.90.9 0.950.95 0.10.1 0.130.13 0.150.15 pHpH 4.74.7 5.05.0 5.65.6 6.26.2 6.36.3 6.86.8 7.27.2

표 2에서 보는 바와 같이, 효모는 식품공장 폐수 내의 유기산을 분해하여 유기탄소의 농도를 저하시킨다.As shown in Table 2, the yeast decomposes the organic acid in the food factory wastewater to lower the concentration of organic carbon.

본 발명에 따르면, 효모균을 이용하여 유기물 폐수를 처리함으로써 종래의 방법에 비하여 보다 경제적이고, 효율적으로 폐수의 유기산의 농도 및 총 유기탄소의 양을 감소시킬 수 있다. 또한 잉여로 생산된 효모를 부산물로서 사료등으로 사용할 수 있다.According to the present invention, by treating the organic wastewater by using yeast, it is possible to reduce the concentration of organic acid and the total amount of organic carbon in the wastewater more economically and efficiently than the conventional method. In addition, yeast produced in surplus can be used as feed by-product as a by-product.

Claims (2)

유기물 폐수의 처리 방법에 있어서, 유기물을 유기산으로 전환시키는 단계와 상기 유기산을 탄소원으로 하는 효모를 폐수에 첨가하여 배양함으로써 유기산을 분해하는 단계를 포함하여 이루어 지는 것을 특징으로 하는 효모를 이용한 유기 폐수의 처리방법.A method for treating organic wastewater, the method comprising the steps of converting an organic substance into an organic acid and decomposing the organic acid by cultivating by adding a yeast containing the organic acid as a carbon source to the wastewater. Treatment method. 제 1 항에 있어서, 유기물 폐수를 처리한 후 효모를 회수하여 사료로 사용하는 것을 특징으로 하는 효모를 이용한 유기 폐수의 처리방법.The method of treating organic wastewater using yeast according to claim 1, wherein after treating the organic wastewater, the yeast is recovered and used as feed.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ305928B6 (en) * 2005-07-13 2016-05-11 K.R.K. Hájek, s. r. o. Conditioned product for manufacture of reclamation material

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CZ305928B6 (en) * 2005-07-13 2016-05-11 K.R.K. Hájek, s. r. o. Conditioned product for manufacture of reclamation material

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