KR20020004300A - Jade extractant And Process For Preparing Thereof - Google Patents

Abstract

PURPOSE: A jade extract and a producing method thereof are provided, therefore the jade extract can be effectively used in various diseases including headache, insomnia, indigestion and dull pain of a hand and a foot. CONSTITUTION: The jade extract is produced by pretreating jade by breaking, selecting and washing it; inserting the pretreated jade with purified water into a dryer in a ratio of 1:4; heating them at 100 deg.C; collecting the distilled jade component-containing vapor; and converting it into a jade component-containing water by heat-exchange, in which the jade extract contains 34% of silicone, 10% of magnesium, 4.9% of calcium, 0.23% of iron, 0.16% of aluminum, 0.17% of copper, 0.046% of cobalt, 0.14% of manganese, 0.024% of tin, 0.00072% of beryllium, 0.0013% of silver, 0.0038% of titan, 0.0028% of nickel and 0.0030% of chrome.

Description

Jade extractant and its preparation method {Jade extractant And Process For Preparing Thereof}

The present invention relates to a jade (Jade) extract and a method for manufacturing the same, in particular, jadeite mining light of the fine fibers of the hollow fiber-yang yangseok series of fine fiber fibers (직 顯 구조 structure) Chuncheon) Jade and ore mined in pure purified water and distilled, and by purifying the jade water vapor generated at this time is related to Jade extract (Jade) extract and the method for producing the extract material.

In general, jade is roughly divided into jadeite and nephrite, and jadeite is a lead masonry mineral belonging to the pyroxene family. Soda is a monoclinic substance, hardness is a dense mass like crystal, light is black, cyan, green, transparent or translucent, and jade usually refers to jadeite. .

Purgatory is an inosilicates monolithic whiskey mineral, which is divided into purgatory and serpentine superbasic purgates in high-quality surrogate rocks, and their quality is characterized by their fine structure, Hollow-Grilled Stone-Yang Ki-Suk is determined by the speed of fastening and fiber, and it is known that the thinner the better the quality (Mining Promotion 1993 New Year's Issue: Korea Mining Promotion Corporation).

According to a report published in the German medical literature regarding jadeite and jadeite ("KRAFT der KRISTALLE und der EDELSTEINE") by MaudaPalmer Die Verborgene, jadeite and nephrite are two different ores, which, like most gemstones, Although jadeite contains oxygen, the jadeite is formed of granular crystals, whereas jadeite is composed of countless crystals such as fibers and hair and fine aggregates. Especially, jadeite is different from the sodium and aluminum components of jadeite. It has been reported recently to have three minerals that are beneficial to the human body, namely calcium, iron, and magnesium, which greatly affect the healing of diseases caused by high blood pressure, diabetic circulatory disorders, heart disease and kidney disorders when worn with purgatory.

Also, in Dongbobogam, a classic of oriental medicine, jade is added to Omiju and Jiyuju, and it turns into water, and it is good to eat when it is put in Honjangsu. When eaten in the same size as 大, the waste is discharged to the outside of the body, 1 oxol, 1 jijicho (地 楡 草), 1 bream (1 米), and 3 egret (白露) are mixed together and put in a copper bowl. If you build a temple, the oxol turns into water, which is called oxal (玉液) and is called Shinseonokjang (神仙 玉 漿), and it is called Shin Nongwoncho, Dangboncho, and Boncho Rivers. According to the jade powder to make the enlightenment and taking it not only has the effect to make the five intestines rich and to completely discharge the waste in the body, but also to remove the heat in the stomach (胃) is effective in the digestive system , Bronchial asthma and fever and chest pain Good, thirst-quenching, sesame-like blouse, the body will fly and lighten, long-lived, lubricating the lungs, vocal cords, good for throat, nourish the hair, function of the five intestines It also helps to relieve stressful neurological disorders.In addition, when the muscles are tense and the rats fly, white jade is eaten as powder.When there are scar marks on the face and body, rubbing the wounds with purgatory for several days will cause scars. Purgatory, the main ingredient of purgatory has been known since early to have an excellent effect on the human body with little side effects.

Although purgatory is known to have such excellent medical effects due to its scarcity, it is only limitedly used as some jewelry for jewelry, such as necklaces, rings, and wrists. It is not possible to work on it unless it is a skilled person who requires careful attention and it is expensive, and since it is expensive, it is economically disadvantageous and there is almost no development to use purgatory generally. Therefore, its research and development is urgently needed. It is at the required time. Applicant paying attention to the excellent medical efficacy of the purgatory as described above, the applicant has a number of domestic and foreign patents other than "glass products containing jade powder and its manufacturing method" (Patent No. 112812).

The present inventors have focused on the fact that purgatory imparts such beneficial effects, and have completed the results of long research and experiment.

An object of the present invention is to provide evaporator with pure purified water and jade ore (cross-linked fiber brown rice structure, which is very thin). It is heated in a high temperature, and the steam generated at this time is heat-exchanged and converted into an extract purgatory distillate, so as to cure diseases of the human body (headache, insomnia, indigestion, numbness of the hands, etc.), growth and growth ability of animals and plants. It is to provide a jade chulu material and its manufacturing method that exhibited medical properties.

Jade extract of the present invention in order to achieve the above object is jade ore and pre-prepared purified water through a pretreatment process such as crushing, screening and washing related to the invention of claim 1 in a ratio of 1: 4 and then 100 ℃ It is characterized in that the above-mentioned high-temperature heating, the ore-containing material eluted in the purified water when heated to evaporate with water vapor, such as purified water, and the water vapor containing the purifying ore-containing material is converted into a nephrite distillate through heat exchange .

In addition, in order to achieve the above object, the preparation method of the jade extract of the present invention is a pre-treatment step of crushing, screening and washing the mined purifying ore according to the invention of claim 3 to a predetermined size, preparing a purified water, and Putting the ore and purified water in an evaporator at a ratio of 1: 4 and heating it at a high temperature of 100 ° C. or higher to allow the ore-containing material eluted in the purified water to evaporate with purified water, and the ore generated in the evaporation step. And converting the water vapor containing the substance into a distillate through heat exchange.

The jadeite ore is defined as a butch δ ¹⁸ 0 as a hollow-trillite jadeite in a high quality surrogate rock, and is formed as follows.

Semi-Quantitative Analysis (%) of Purified Ore of the Invention

Silicon 34

Magnesium 10

Calcium 4.9

Iron 0.23

Aluminum 0.16

Copper 0.17

Cobalt 0.046

Manganese 0.14

Tin 0.024

Beryllium 0.00072

Is 0.0013

Titanium 0.0038

Nickel 0.0028

Chromium 0.0030

Miscellaneous 0

In addition, in the above ratio of the purgatory ore and purified water, the more the purgatory ore, the higher the inherent efficacy of the purgatory, but due to the higher price of the purgatory ore, the ratio of purified water and the ratio of 1: 4 is appropriate.

In addition, the purified water used above is not particularly limited, but it is preferable to use one that does not contain impurities which limit the properties of possible purifying ore.

1 is a view schematically showing an apparatus for preparing jade extract according to the present invention,

2 is a crystal structure diagram showing a purgatory powder used in the present invention by a scanning electron microscope (SEM),

3A and 3B are typical chromatogram diagrams obtained from standard solution (A) and microdialysis sample (B) used in the present invention.

Figure 4 is a tissue diagram of the microdialysis probe site located in the striatum (top) and side nucleus (bottom) used in the present invention,

5 is a graph showing the effect of purifying distillate (extract material) on the dopamine glass by metamphetamine in the striatum used in the present invention,

Figure 6 is a graph showing the effect of nephrite distillate (extract material) on dopamine glass by nicotine in the striatum used in the present invention.

* Explanation of symbols for main parts of drawings *

100: purgatory evaporator 102: purgatory ore

104: purified water 106: evaporation vessel

110: evaporation duct 120: heat exchanger

Hereinafter, preferred embodiments of the jade extract material and the preparation method according to the present invention will be described in detail.

1 is a view schematically showing an evaporation apparatus 100 for preparing jade extract quality according to the present invention, with reference to this drawing will be described an example of the preparation of jade extract quality.

First, Chuncheon mountain jade gemstone prescribed as Butch δ ¹⁸ 0 as a hollow calcite in high-quality surrogate rock is crushed using a general crusher, and then sorted through a sorting process, and then washed. By completing the pretreatment step.

The jade ores 102, which have been pretreated in this way, are placed in the evaporation vessel 106 at a ratio of 1: 4 with purified water 104 containing no pre-prepared impurities. Thereafter, when the ignition nozzle 108 positioned below the evaporation vessel 106 is operated, the evaporation vessel 106 is continuously heated at a high temperature of at least 100 ° C. by the high temperature at this time. As a result, the contents inside the evaporation vessel 106 is in a severe boiling state. When boiled like this, the substance (silicon, magnesium, calcium, .. etc.) of jade ore itself eluted from jade ore 102 to purified water 104 is converted into water vapor such as purified water 104 and then discharged into duct 110. When passing through the discharge duct 110, the water vapor is converted into a liquid phase by the temperature difference with the inside of the evaporation chamber flows along the passage is disposed on the outlet side of the discharge duct 110 ( 200 is collected into the interior. The water vapor passing through the discharge duct 110 as it is is converted into a liquid phase while being heat-exchanged at the moment passing through the heat exchanger 120 installed adjacent to the discharge end of the discharge duct 110. The purified water is As the evaporation time elapses, it needs to be replenished continuously. In addition, since the heat exchanger 120 has a general configuration, a detailed description thereof will be omitted. Jade extract material, that is, distillate obtained by the above method, is used as an adjuvant for promoting metabolism of the human body or for the treatment of diseases, and the experimental results are as follows.

Experimental Example 1

Experimental examples of Tables 1 to 4 below are experiments on the efficacy of purgatory used in the present invention and the effect of the purgatory on the living body.

Lead Content Test sample Jade minutes Sample property White powder Job number Federal Institute of F.D.A.American Studies IW 091394-1 Way Atomic absorption analysis result Not detected

<Heavy Metals (Pb, etc.)> sample Jade powder Sample property White Job number Federal IW 080894-4 Way USP 23 result Not detected

<Inorganic elution test> sample Jade powder Sample property White Job number Federal IW 080894-4 Way As result As

100 g of the sample was extracted with 1 liter of water by autoclave treatment, and the extract liquid was analyzed.

Analyte Results (ppm) Detection limit (ppm) Arsenic (As) ND 0.05 Barium (Ba) ND 0.20 Cadmium (Cd) 0.007 0.005 Chlorine (Cl) ND One Chrome (Cr) ND 0.01 Copper (Cu) ND 0.05 Fe ND 0.10 Pb ND 0.05 Manganese (Mn) ND 0.02 Mercury (Hg) ND 0.0005 Nitrate (NO3) ND 0.1 Selenium (Se) ND 0.05 Silver (Ag) ND 0.01 Sulfate (SO4) ND One Zinc (Zn) ND 0.01

(ND = concentration is below detection limit or not detected)

As shown in the experimental results, purgatory used in the present invention was proved to be safe when used in plants or animals, for example, since no harmful lead, heavy metals, or other toxic components were detected.

Experimental Example 2

In this experimental example, far-infrared experiment on purgatory was performed (FIG. 2).

sample Purgatory powder Sample property White method of inspection Perkin elmer 137 result As

<In IR spectrum>

Form: Tetrahydrofran Liquefied Thin Film

It appeared to match the pattern of polycarbonate resin and radiated electromagnetic waves with a wavelength of 6-52μ.

Experimental Example 3

In this experimental example, the experiments were performed as shown in Table 6 and Table 7 below for the chemical and biological oxygen demands for the soft oxidants used in the present invention.

sample Jade minutes Sample property White Way Standard method result Same as Table 7

division Water (control) Jade addition 5-day BOD 224 mg / l 223 mg / l COD 115 mg / l 110 mg / l

The following experiment (Experimental Example 4) shows the effect of purgatory distillate, that is, purgatory extract, obtained by the above example on the brain function according to the results proved by the above experimental examples. The experiment was commissioned by the medical doctor), the experimental method and results are as follows.

Experimental Example 4

A. Effect of Jade Extract of the Present Invention on the Dopamine Release by Metamphetamine

1) Experiment animal

In vivo microdialysis experiments were performed using male Sprague-Dawley rats (280-320g, Korea Experimental Animal Center). The animals had free access to food and water and were kept in a controlled breeding room with a 12:12 hour day and night cycle.

2) in vivo microdialysis experiment

a) medication

To investigate the effect of jade extracts on dopamine-induced methamphetamine release, 0.5 ml of nephrite extract (purine distillate) of the present invention was administered intraperitoneally 60 minutes prior to administration of methamphetamine (10 mg / kg intraperitoneal injection). After injection the dialysate was collected from the striatum.

b) procedures and microdialysis

Experimental animals were anesthetized using pentobarbital (50 mg / kg intraperitoneal injection) and then sterile (as stereoscopic coordinates: AP 1.0, L3.2 for Bregma) according to the atlas of Paxinos and Watson (1986). , Induction tube was inserted into the dura mater and fixed using a microscrew and dental cement. After 24 hours of recovery, 4 mm vertical microdialysis probes (CMA-12, Carnegie-Medicine, Stockholm, Sweden) were inserted through the induction tube. The inlet of the microdialysis probe is connected to a syringe mounted on a perfusion pump via a double-ring rotator, through which the cerebrospinal fluid (145 mM NaCl, 2.7 mM KCl, 1.2 mM CaCl ₂ 1.0 mM MgCl _2, 2.0 mM Na ₂ HPO). _4; pH 7.4) was injected at a rate of 1.5 μl / min, and dialysate was collected every 20 minutes using a microfraction collector (Carnegio-Medicin) connected to the probe outlet. Microdialysis experiments were conducted on freely active experimental animals.

c) microdialysis sample analysis

The concentrations of mono amines and their metabolites in the microdialysis samples were analyzed using an HLPC-electrochemical detector (ECD) system. The dialysate (injection 30 μl) was subjected to a reversed-phase Waters Nova-Pak C-18 column (4 μm). , 150 x 3.9 mm). The mobile phase consisted of 75 mM sodium phosphate, 0.1 mM EDTA, 1.4 mM octansulfonic acid, and 10% acetonitrile and was adjusted to pH 3.2 with phosphoric acid. HPLC peaks were analyzed using an ESA Coulochem II 5200A electrochemical detector equipped with a high performance analyzer (ESA model 5014) and the electrode voltage was set to + 320mV. The flow rate of the system was 1.0 ml / min.

Typical chromatograms obtained from standard solutions and microdialysis samples are shown in FIG. 3. Dopamine, dihyddroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT), 5-hydroxy indole acetic acid (5-hydroxyindoleacetic) acid, 5-HIAA) were clearly separated. The dopamine concentration of the dialysate was more than three times the minimum measurable value (116 pM). Dopamine, DOPAC, HVA, 5-HT, 5-HIAA basal concentrations of the dialysate were 18.5 nM, 90.5 nM, 74.7 nM, 6.2 nM and 45.6 nM, respectively. The extracellular fluid dopamine concentration was estimated to be 92.5 nM because the dopamine recovery was 20%. Dopamine concentrations of the dialysate are expressed as percentages based on the average dopamine concentrations of the last three samples collected immediately before the experimental treatment.

d) systematic confirmation

After the end of the experiment, deep anesthesia was induced with pentobarbital, and the brain was removed after perfusion of the experimental animals with physiological saline and 10% formalin solution. The removed brain was stored in 10% formalin solution for at least 2 weeks, and then the tissue at the site of the microdialysis furrow was cut to a thickness of 50 μm to confirm the position of the probe by hematoxylin staining (see FIG. 4).

e) statistical analysis

It is shown by the mean of measurement ± standard difference. Statistical significance of the data was analyzed using repeated measures binary variance (drug X time) and Bonferroni method. Statistical analysis software StatView 4.02 (Abacus Concepts, Inc., Berkely, CA, U.S.A.) and a Macintosh computer were used. Statistical significance was determined based on P value 0.005.

3) Research Result

In Fig. 5, the effect of nephrite extract (distillate) on dopamine release by metamphetamine in the striatum is shown. After administration of methamphetamine (10 mg / kg intraperitoneal injection), the stratified extracellular fluid dopamine concentration gradually increased to its maximum after 60 minutes (433.6 ± 14.3% of the base value (mean ± standard error)). Intraperitoneal injection 60 minutes prior to methamphetamine administration resulted in a 32.8% decrease in maximal stratified extracellular dopamine concentrations by metamphetamine (29.15 ± 11.4% of baseline). Purgatory distillate administration also delayed the time to reach the maximum dopamine concentration after methamphetamine administration (80 minutes after methamphetamine administration). On the other hand, the nephrite distillate of the present invention did not affect the concentration of dopamine in the striatum stratified extracellular fluid.

Effect of Jade Extract on Dopamine Release by Nicotine

1) experimental animals

In vivo microdialysis experiments were performed using male Sprague-Dawley rats (280-320g, Korea Experimental Animal Center). The animals had free access to food and water and were kept in a controlled breeding room with a 12:12 hour day and night cycle.

2) in vivo microdialysis experiment

a) medication

To investigate the effect of jade extract on the dopamine release by nicotine, the dialysis material was collected from the striatum after intraperitoneal injection of 0.5 ml of nephrite distillate 60 minutes before nicotine (0.4 mg / kg subcutaneous injection). .

b) procedures and microdialysis

Experimental animals were anesthetized using pentobarbital (50 mg / kg intraperitoneal injection), and then sterile (stereotropic coordinates: AP1.0, L3 for bregma) according to the atlas of Paxinos and Watson (1986) by aseptic methods. 2, induction tube was inserted into the dura mater and fixed using a microscrew and dental cement. After 24 hours of recovery, a 4 mm vertical microdialysis probe (CMA-12) was inserted through the induction tube. The inlet of the microdialysis probe is connected to a syringe mounted on a perfusion pump via a double-ring rotator, through which the cerebrospinal fluid (145 mM NaCl, 2.7 mM KCl, 1.2 mM CaCl ₂ 1.0 mM MgCl _2, 2.0 mM Na ₂ HPO). ₄ ; pH 7.4) was injected at a rate of 1.5 μl / min and dialysate was collected at 20 minute intervals using a microfraction collector (Carnegio-Medicin) connected to the probe outlet. Microdialysis experiments were conducted on freely active experimental animals.

c) microdialysis sample analysis

The concentration of microdialysis samples was analyzed using an HPLC-ECD system. The dialysate (injection volume 30 μl) was separated using a reversed Waters-Pak C-18 column (4 μm, 150 × 3.9 mm). The mobile phase consisted of 75 mM sodium phosphate, 0.1 mM EDTA, 1.4 mM octan sulfonic acid, and 10% acetonitrile and adjusted to pH 3.2 with phosphoric acid. HPLC peaks were analyzed using an ESA Coulochem II 5200A ECD equipped with a high performance analyzer (ESA model 5014) and the electrode voltage was set to +320 mV. The flow rate of the system was 1.0 ml / min. Dopamine concentration of the dialysate is expressed as a percentage based on the average dopamine concentration of the last three samples collected immediately before the experimental treatment.

d) histological confirmation

After the end of the experiment, deep anesthesia was induced with pentobarbital, and the animals were perfused with saline and 10% formalin solution through the heart to remove brains. The removed brain was stored in a 10% formalin solution for at least 2 weeks, and then the tissues of the microdialysis probes were cut to a thickness of 50 μm to confirm the position of the probes by hematoxylin staining.

e) statistical analysis

Measurements are expressed as mean ± standard deviation. The statistical significance of the data was analyzed using repeated measures binary variance analysis (drug X time) and Bonferroni method. Statistical significance was determined based on the P value of 0.05.

3) Research Result

Figure 6 shows the effect of purgatory extract (distillate) on dopamine release by nicotine in the striatum. After administration of nicotine (0.4 mg / kg subcutaneous injection), the stratified extracellular fluid dopamine concentration gradually increased to its maximum value after 40 minutes (403.9 ± 33.2% of the mean value (mean ± standard error)), and then decreased after 120 minutes. Returned to baseline levels. Intraperitoneal injection of 0.5 ml of nephrite distillate 60 minutes before nicotine administration resulted in a 52.0% decrease in the concentration of the extracellular extracellular dopamine by nicotine (193.9 ± 6.1% of baseline). In addition, administration of nephrite extract (distillate) delayed the time to reach the maximum dopamine concentration after nicotine administration (80 minutes after nicotine administration). Purgative extract (distillate) did not affect the concentration of dopaminergic extracellular fluid dopamine.

From the above experimental results, it was confirmed that purgatory extract of the present invention inhibits dopamine release by metamphetamine and nicotine in rat striatum.

In this experiment (Experimental Examples 5 to 6) to investigate the effect on the reproduction and growth rate of rats when treated with jade distillation solution of the present invention, jade water in which the jade material and the jade water precipitated The experiment was commissioned by the Korea Food Research Institute. Details are as follows.

Experimental Example 5

This experiment was performed by the Korea Food Research Institute to investigate the effect of various purgatory on the symptomatic relief of streptozotocin-induced diabetes in rats. Details are as follows.

32 spragdoli mice weighing 252-292 grams were intraperitoneally administered ST (streptozotocin) (35-40 mg / kg body weight dissolved in 0.1 M citric acid in pH 4.0) to induce diabetes It was. After 7 days of ST administration, using only europaper (Eiken Chemical Co., Ltd., Japan), only urine glucose over 250 mg / dl was selected and 5 mice per treatment group was placed for 50 days. Animals in group A were injected intraperitoneally with 1.0 ml of nephrite distillate (pH 6.40) of the present invention. Group B was raised in a cage fed with meteorological jadeite. Group C was fed daily with supernatant of purgatory water after precipitation of purgatory powder, and group D was administered only ST as control. Experimental feed was fed commercially available feed, the breeding method was in accordance with the usual practice. Mice were sacrificed at the end of the experiment (50 days) and blood was collected from the abdominal aorta. The blood was left at room temperature for 30 minutes and then centrifuged at 5,000 rpm for 15 minutes to obtain plasma. The weights of organs such as liver and kidneys were weighed and recorded.

Plasma β-hydroxybutyrate (HBA), free fatty acids (FFA), cholesterol, HDL-cholesterol and triglycerides were measured. The HBA content was quantified by measuring the absorbance (OD) increase at 340 nm of NADH produced by the oxidation reaction with β-hydroxybutyrate dehydrogenase, and the blood lipids were measured in clinical trial kits (Eiken Chemical Co., Ltd., Japan).

Effect of Purgatory on Body Weight and Organ Weight in Streptozotocin-Induced Diabetic Rats Treatment group Body weight (g) Organ weight (g / 100g body weight) Before experiment After the experiment liver kidney A 270.4 ± 11.3 271.7 ± 49.4 4.31 ± 0.33 1.02 ± 0.12 B 284.8 ± 6.1 279.6 ± 20.4 4.30 ± 0.64 1.01 ± 0.18 C 280.4 ± 13.2 315.7 ± 41.1 3.96 ± 0.75 0.87 ± 0.14 D 257.2 ± 4.2 194.8 ± 26.3 4.37 ± 0.19 0.78 ± 0.04

Purgative effect on biochemical index of streptozotocin-induced diabetic rats Treatment group Glucose (mg / 100ml) HBA ^a (μmol / ml) FFA ^b (μeq / ml) TC ^c (mg / 100ml) TG ^d (mg / 100ml) HDL ^e (mg / 100ml) A 639.2 ± 99.0 ^a 0.782 ± 0.481 ^a 507.6 ± 226.6 ^a 166.2 ± 69.4 410.7 ± 50.1 32.35 ± 5.94 B 495.1 ± 228.1 ^ab 0.527 ± 0.296 ^ab 349.5 ± 79.2 ^ab 161.3 ± 45.3 122.5 ± 43.5 29.49 ± 1.71 C 354.1 ± 154.2 ^bc 0.425 ± 0.172 ^ab 287.3 ± 71.7 ^b 145.4 ± 29.1 87.9 ± 29.2 30.55 ± 8.28 D 196.8 ± 16.3 ^d 0.276 ± 0.036 ^b 291.0 ± 131.2 ^b 158.2 ± 12.2 86.8 ± 3.4 31.37 ± 3.84

p <0.05

^a βhydroxybutyrate (ketone body)

^b free fatty acids

^c total cholesterol

^d triglyceride (neutral lipid)

^e high density lipoprotein cholesterol

Body weight was increased in group C fed purer corn, while it was decreased (B, D) or maintained in other groups (A). In diabetic rats, renal hypertrophy is known to be a common symptom. Similar trends were observed in this study, and kidney weight in group C was the lowest among the treatment groups except the control group. Liver hypertrophy was also caused by diabetes, but liver weight was the lowest in rats fed corn. The concentrations of blood glucose, ketone bodies and FFA were highest in the order of A, B, C, and D treatment groups. Plasma cholesterol and triglyceride concentrations showed the same trend (A> B> C> D). From the above results, rats fed the nephrite corn (group C) showed relatively lower values of all the indexes than the other two experimental groups, and blood cholesterol and FFA concentrations were lower than those of the control group without the nephrite treatment. Blood cholesterol (TC) and neutral lipid (TG) concentrations were generally higher in the order of A, B, C, D treatment groups. When the glycemic index was determined by the post-prandial blood glucose response, the experimental results may vary according to the investigator. However, in the present experimental example, a commercially available complete formula (TMR) without considering the glycemic index of cereals in the experimental feed was considered. Even though they were fed, the treatment group (group C) fed agate improved the hyperlipidemia, one of the symptoms of diabetes. HDL-cholesterol content did not differ between treatment groups. In conclusion, when the meteorological purifying substance and nephrite distillate were fed to streptozotocin-induced diabetic rats, not only the weight loss but also the renal or hepatic hypertrophy and the hyperlipidemia, a type of diabetes mellitus, were improved.

Experimental Example 6

Sprague Dawley rats, 9 weeks old, were prepared. The rats of group A were allowed to drink tap water in a cage filled with air containing the gaseous nephrite, and the rats of group B were supernatant after immersing the nephrite powder (2 mg / ml tap water). It was separated and drunk, but it was not placed in the cage that supplied the gaseous purgatory material. In the C group, the control group was free of air or chalcedony containing jadeite. All treatment groups were seeded for 9 days and then males were isolated and slaughtered for sperm concentration and vitality testing. The results are shown in Table 10 below.

Effect of Purgatory Substances on Rat Reproduction and Growth Treatment group A B C Child Birth Number (Marine) 9 9 8 Number of cubs (♂ / ♀) 96 (57/39) 94 (57/37) 102 (54/48) Number of cubs (head count / ♀) 11.8 ± 1.9 10.9 ± 1.4 12.8 ± 2.1 Body weight at birth (g) 4.73 ± 1.10 5.79 ± 0.95 5.35 ± 0.35 Mortality rate (mari) 4 4 3 Time from end to delivery (days) 23.8 ± 1.4 (n = 10) 25.1 ± 1.7 (n = 9) 25.5 ± 3.7 (n = 8) Weaning weight (g) 43.45 ± 8.04 42.73 ± 8.76 42.07 ± 10.71 Body weight at slaughter (g) 294.0 ± 10.9 274.4 ± 8.6 288.4 ± 26.7 Testicle weight (g) 3.90 ± 0.21 3.57 ± 0.30 3.99 ± 0.15 Sperm Concentration (10 ⁸ / ml) 6.32 ± 2.4 4.80 ± 1.3 4.60 ± 1.9 Sperm Motility (%) 91.0 ± 4.2 92.0 ± 2.7 88.0 ± 7.6

Experimental results showed that the A and B corn group treated with jade material showed better delivery results than the control group. The delivery rate was 90% in the A and B treatment groups and 80% in the C treatment group, and the number of cubs was 6-8 in the control group, but the number of males was 3 in the A and B treatment groups. There were more horses. The days to postpartum delivery were significantly shorter in the A treatment group. In addition, sperm concentration and vitality of sperm were excellent in the A group treated in the cage fed with jade. However, the effects of simultaneous treatment of jewels and corn are not studied in this experiment. In conclusion, the above results suggest that jadeite and agate affect rats' reproduction and growth, and inhaled jadeite or agate treated group showed better breeding and growth index. It is expected that a lot of valuable research will be performed on these phenomena. However, these results are considered to be valuable as basic data for improving reproductive efficiency and enhancing sperm concentration and motility.

In the following experiments (Experimental Examples 7 to 9), the effect of Chuncheon nephrite jade and the jeongjeongsu obtained therefrom on the germination and growth of seeds was examined.

A. Component Analysis of Purgatory and Okjeongsu

The PW1,480 X RIW-Fuorescence Seguenflal Spectrometen spectrometer was used to analyze the components of Chuncheon Purgatory Purgum, which revealed that SiO2 was the main component and contained some trace elements necessary for plant growth.

B. Water Quality Analysis of Jade and Groundwater

Water quality analysis of Okjeongsu (Ok Minsan groundwater) and groundwater was conducted. As a result, no unusual trace elements were found in the underground water of Okwang Mountain.

Experimental Example 7

<Comparison of the Effects of Jade Essence on Germination of Seeds>

① The germination experiment was to select 20 seeds from balsam, soybean, rice, radish, garland chrysanthemum, and seeds, and put 8 sheets of toilet paper on the chalet to replace the seeds and to supply okjeongsu, groundwater, and tap water.

② The germination rate of five days of storage at room temperature cows was compared and observed five times at a distance of 50m from the seed that supplies okjeong water and the chalet that supplies ground water tap water.

③ In order to study the range of oksujeongsu, one chalet of 20 radish seeds and rice seed was supplied with okjeongsu, and the other chalets were installed 10m, 20m, 30m, 40m, and 50m away, and ground water was supplied to test germination results. .

The results are shown in Table 11 and Table 12.

<The germination rate by tap water and ground water tap water> Water quality Jade Jeong Soo Groundwater tap water Remarks 2 3 4 5 2 3 4 5 2 3 4 5 rice plant 10 45 85 96 6 43 76 91 4 42 74 90 radish 27 78 90 96 26 73 87 94 24 72 86 94 Garland chrysanthemum 20 46 64 64 18 45 60 61 18 41 61 62 pea 9 23 48 51 9 21 47 51 8 20 43 52 Chinese cabbage seeds 28 79 91 91 24 73 90 90 23 71 90 90

As a result of the experiment, it is judged that Okjeongsu accelerates the germination period of the above five seeds.

<Comparison of Moose Germination Rates by Okjeong Water and Ground Water Supply> Installation distance elapsed Jade Jeong Soo 10 m 20 m 30 m 40m 50 m Remarks One 8 7 8 5 6 6 2 27 28 29 22 21 22 3 78 79 77 75 74 73 4 90 89 89 90 89 89 5 96 95 94 93 94 93

As a result of the experiment, it can be said that the Aura of Okjeongsu affects up to about 20m, and it is judged that Okjeongsu's group accelerates the germination period of the seedless.

Experimental Example 8

<Comparison of the Effects of Jaggies on Seed Germination>

Spread 8 pieces of toilet paper on 5 jaggies and 25 chalets, and obtain 20 seeds of balsam, pea, rice, radish and garland chrysanthemum. Observation experiments were carried out considering the range of energy (see Table 13).

① The germination rate was compared five times for five days in the cow.

② Statistics are based on the sum of one to five times (100%).

<Germination Comparison of Seeds by Okjagi (Number of Germinations)> Installed distance Jade Jagi 10 m 20 m 30 m 40 m 50 m 3 5 3 5 3 5 3 5 3 5 3 5 rice plant 51 90 45 85 46 91 49 92 40 92 41 88 radish 90 96 88 95 88 94 89 95 81 94 82 95 Garland chrysanthemum 54 70 51 58 50 71 49 69 45 69 46 70 pea 25 60 24 61 24 58 23 60 20 59 19 58 Chinese cabbage seeds 74 93 72 95 74 96 71 94 69 94 68 93

As a result of the experiment, it can be said that Gi (기) affects about 30m and accelerates germination time of stomach seeds.

Experimental Example 9

<Comparison of Soybean Sprout Growth Rates of Jade and Okjeongsu>

Root sprouts and onions were tested to examine the effects of jade and jeongjeongsu on plant growth.

① Purchased a bowl for growing bean sprouts, put 30 bean sprouts each, and supplied okjeong water, ground water and tap water at room temperature, and compared germination rate and growth rate for 10 days.

② Put bean sprouts 30 pieces in a porcelain, general porcelain, plastic bowl, and compared the growth rate and germination rate at room temperature for 10 days.

③ Obtained three onions of the same size and compared the rooting by supplying Okjeongsu, groundwater, and tap water.

④ 50m away from Okjagi and Okjeongsu, respectively.

The results were as follows.

1) Sprouts growth by jade

Okjagi creeping has been found to affect growth rate because it accelerates seed germination.

2) Growing bean sprouts supplied with jade crystal water

Ok jeong-soo's creeping has been shown to affect the growth rate because it speeds up the germination period.

Experimental Example 10

<Comparison of Freshness of Plants by Jade and Okjeongsu>

① We compared freshness by supplying groundwater by inserting flowers of the same kind to jade and general porcelain.

② Check the freshness of the glass by supplying jeongjeong water, ground water and tap water.

③ They were installed at a distance of 50m away from the bowls which were not the same as Okjagi and Okjeongsu.

④ The flowers were cut out of three flowers, such as roses, carnations, and chrysanthemums, at the same time, and selected as the same flower in full bloom, and visually observed once a day.

The results were as follows.

1) Freshness of jade and plants

Okjagi creeper is thought to affect the freshness of plants slightly

2) Freshness of Jade Water and Plants

Okjeongsu's creeper is thought to have some effect on plant freshness

Experimental Example 9

Effect of Purgatory Powder on Plant Growth

① Planted 50g of purgatory powder and 3g of fine sand, planted three seeds of Gangnam beans, peas, radish, and Chinese cabbage and compared the growth rate of the plants.

② fertilization problem is difficult, radishes and cabbage did not fertilize. However, the beans and peas were sown in the same amount of mixed soil like sand.

③ Purgatory powder plant and general pots are installed at 50m intervals apart.

The results were as follows.

1) pea growth

Purgatory powder (AURA) is thought to have a slight effect on growth because it accelerates seed germination.

2) Growth Comparison of Gangnam Beans (m)

Purgatory powder is thought to have a slight effect on growth because it speeds up the germination of seeds.

3) Hydroponic Cultivation of Onions

Ok jeong-soo's creeper is thought to have a significant effect on onion rooting

4) Growth rate of radish

Purgatory powder affects the germination timing of seeds, which is thought to promote growth rate

As confirmed in the above experimental example,

(1) Chuncheon nephrite has a hardness of 6-6.5 and all δ18O is 負 値, and the measurement of δD of purgatory jade is controlled by the hydroxyl group. ) Is not in the range (δD% = 0 to -70).

② As a result of analyzing the purgatory, it can be seen that the ingredients vary depending on where the sample is taken.

③ Aura emitted from purgatory and jade porcelain can affect the germination and growth of plants up to about 30M.

④ The jeong of Okjeongsu is slightly weaker than the jade of purgatory can be seen that affects the germination and growth of plant seeds up to 20M-30M,

⑤ In the seed germination experiment, there was a slight difference between the control and treatment groups in both Okjeongsu and Okjagi.

⑥ It can be seen that growth is promoted by purgatory powder in all radishes, peas, gangnam beans and Chinese cabbage.To summarize the results of the experiment, it can be used for hydroponic cultivation by improving the fertilization or acidification with acidified water using jade water. This suggests that it can be used as a neutralizer for drinking water and as a growth promoter for removing concentrated heavy metals.

In the following experiments (Experimental Example 11), mainly for marine microorganisms, jade products made from jades collected in chuncheon nephrite ore such as nephrite ore used in the purgatory extract of the present invention (octale, jade, jade, Influence of survival and reproduction in the presence of oxom).

Experimental Example 11

(1) .Microorganisms

In this experimental example, the marine bacteria were used in Daecheon seawater, which is mainly used for the production of medium. The seawater was preserved in the cow for 6 months and used in aged sea water, a state in which most organic matters were decomposed. The used photoluminescent bacterium, Photobacterium Phosphoreum, is Gram (-) bacterium and used 1.4 × 1014 quanta / sec of luminescence intensity per ml. We measured the luminescence of 660 nm using the spectrophotometer (Milton-Roy MR 3000) and the luminescence of 1 ml using the luminometer, respectively.

(2) .Use medium

As the medium used, ZoBell medium and sea water complete medium, which have been used for a long time in culture of marine bacteria, were used. The composition of the medium is as follows.

<Zobell badge>

Bactotryptone 3 g

Yeast extract 1 g

0.1 g FeCl3

Aged seawater 700 ml

300 ml of distilled water

<Sea Water complete medium>

Bactotryptone 5 g

Yeast extract 3 g

3 ml of Glycerol

Aged seawater 750 ml

250 ml of distilled water

Petri-film: 3M

The medium used for colony forming unit (CFU) was Peri-film purchased from 3M, and 1 ml of the sample was added to the film, pressed in a circle, incubated for 3 days, and formed into red cells. At the same time, the results were recorded with a digital camera (Kodak DC-120).

Octal, jade disk, jade powder, jade stone, oxom, etc. used in the experiment was used Chuncheon acid nephrite (manufactured by Oxanga Co., Ltd.) samples, and each experimental group was separated and incubated in a 100 ml culture flask. Jade powder was used 1g per 100ml of seawater and jade ore was cut into 1g size. Oxom was also used based on the total weight of 1g.

(3) .Experiment

end. pH change experiment

Sea water 100ml as control

add 1g of Jade Powder into 100ml Seawater

stirring for 30min with magnetic stirrer

Centrifugation to obtain supernatant

Measure pH change of supernatant

pH change

B, cell count change experiment (general seawater diluted with sterile seawater)

(Get a Growth Curve)

<Solid Badge>

Petrifilm use

Use ZoBell plate

<Liquid medium>

Incubate on Octal

Incubate with jade disk

Cultivation by putting jade ore pieces

All. Luminous intensity change experiment

Samples were used using a full seawater medium with a luminous intensity of 1.4x1014 quanta / sec per 1 ml of marine light-emitting bacterium Photobactrium phosphoreum, and after diluting about 1%, the growth and luminescence intensity of cells were changed over time. The absorbance of 600 nm using a spectrophotometer (Milton-Roy MR3000) and the emission of 1 ml of sample using a luminometer were tested.

(4). Measurement of biomass

Measurement of Biomass Using Firefly luciferase Luminescence System

Biomass can be measured in various ways. In the case of organisms that are not visually distinct from bacteria, ATP (adenosine-5) is a bioindicator commonly included in all living organisms to measure the total biomass including them. -triphosphate) method was used. ATP is an essential element that maintains life in all bacteria and above. It is produced through various kinds of bio metabolism, and is used as a direct fuel in cells, and in dead organisms, it is rapidly decomposed into ADP (adenosine-5-diphosphate). It is a good indicator for measuring biomass. On the other hand, estimating biomass with the naked eye or microscopy makes it difficult to distinguish between living bodies and dead bodies, and in the case of microorganisms, it is difficult to measure the viable but non-cultural (VBNC) cells by using a culture method. In the marine environment, there is a limit that only less than 1% can be cultured by the commonly used culture methods.

In the case of this sample, the killing of biomass on the surface of the jade product is a very important measure, so accurate measurement of such biomass is very important. ATP was measured by extracting sediment into buffer, and there are various chromatographic methods such as HPLC and TLC. However, in this study, the bioluminescence method of firefly that emits light using ATP was used. HPLC or TLC method can get very accurate results, but the pretreatment of the sample is very complicated, which requires a lot of time and effort, and the measurement method is low in detection such as requiring UV absorbance or color development. There is a problem. Firefly luciferase converts luciferin into oxyluciferin by using one molecule of ATP and one molecule of oxygen, and emits light of 530 nm. The luminous efficiency is almost 100%, which is the most efficient bioluminescent system known to date. Measuring the amount of is considered to be the most sensitive measurement except for the use of radioactive isotopes.

(5) .Experiment result

5.1 pH change

In general, the pH of seawater is weakly alkaline. The pH value is about 8.1, and if left for a long time, it gradually changes to neutral due to the metabolism of the microorganisms included, and this phenomenon is well shown in the control of Table 14. In the presence of jade products, a slight pH change was observed immediately (within 1 hour). In the case of jade powder or jade ore, the change in pH was about pH 7.8, which showed weak alkalinity, and in the case of jade tile which was not in direct contact with the solution, this tendency was less like that of the control. This phenomenon changed the acidity of the material dissolved in the jade product into the external solution, and the representative one of them is the effect of Silica (SIO2).

<Change of pH of Seawater by Jade Product> 1 hours 2 hours 3 hours Control 8.18 8.16 8.15 Jade powder 8.01 7.75 7.55 Oxom 8.10 7,80 7.64 Octal 8.12 8.05 7.95 Prison 8.1 8.0 7.8 Jade Wonseok 8.1 8.0 7.8

5. Change in Number of Cells

100 ml of standard seawater was placed in a sterile bottle and each jade product was added to the given amount. 1 ml of each time was incubated with shaking at about 100 rpm, inoculated using Petrifilm, and the number of cell colonies formed by incubating for 3 days was counted (see Table 15).

<Changing Cell Number in Seawater by Jade Products> 1 day later 2 days later 3 days later 7 days later Control 324 336 328 319 Jade powder 284 272 243 217 Oxom 274 265 232 204 Octal 283 274 281 295 Prison 272 261 255 275 Jade Wonseok 269 261 253 282

As shown in Table 15, a significant reduction in marine bacterial counts was observed for all jade products used. Initially, the reduction rate was high in oxome and jade, and after 7 days of incubation, the highest reduction rate was observed in jade and oxome. In particular, the oxome is the most effective, it appears to decrease to about 64% of the control group after 7 days.

5.3 Change in Luminance Intensity

The luminescence intensity of marine luminescent bacteria was not significantly changed by various jade products used in the experiment. The degree of change was less than about 10%, but the overall characteristic tended to decrease in all samples. This indicates that jade products show the characteristics of inhibiting the activity of marine bacteria, and this tendency is the same as the previous experiment. Marine luminescent bacteria are easily attached to agglomerates of organic organisms in seawater and propagate, resulting in luminescent phenomena. See Table 16).

<Changes in Luminescence Intensity of Marine Luminescent Bacteria by Jade Products> 1 hours 2 hours 24 hours Control 100 100 100 Jade powder 94 93 88 Oxom 92 90 85 Octal 95 92 84 Prison 95 90 82 Jade Wonseok 93 90 82

5.4 Surface adhesion

On the surface contained in seawater, bacteria in seawater are commonly attached to form bio-films, and these bio-films act as a base material, and other marine organisms are sequentially attached to cause so-called biofouling.

Where the antimicrobial functions of jade products originate is unknown, but these functions have been identified in the above experiments, and they are valuable if they inhibit biofilm formation by these bacteria.

As a result of this experiment, the degree of adherent bacteria was slightly different, but it was 92-95% compared to the control group, showing about 5-8% of anti-adhesion effect (see Table 17).

<Accuracy of Marine Bacteria on the Surface of Jade Products (Weekly Culture)> Number of bacteria adhered (%) Count x 105 Control 100 3.2 Octal 95 3.04 Prison 93 2.97 Jade Wonseok 92 2.94

(4) .Result

Various clinical trials related to jade's efficacy have been conducted in Korea. It has been reported that jade rings or jade necklaces made of purgatory jade have an effect on the condition of patients in the hospital. The results of this study are recognized to be effective in insomnia, heart disease, hypertension, joint pain, headache, tinnitus and menstrual pain in China. This phenomenon is not limited to the human body, but water from purgatory mines has been reported to affect crops or animals in nearby farmland, as confirmed by the above experimental examples. Although research on specific components of jade causing such a biofunctional enhancement phenomenon has been conducted, until now, it has not been identified which phenomenon is caused by a particular substance. In addition, it is revealed that the jade component or jade product shows the antibacterial effect, and the development of health supplements using the same has been made in various ways. In particular, its use is expected to increase in preservation of food and cultivation of edible organisms. The trend is that it can be used in artificial farming of marine fish, which is increasingly used for food, suggesting that it can be used to control fish disease, the biggest problem of fish farming. Indeed, the mortality rate of fry caused by fish disease in fish and shellfish exceeds 30%, and many antibiotics are used to prevent them. Overuse of these antibiotics not only significantly reduces the value of the fishery products produced, Effective control of fish disease remains an important task to be solved in fish and shellfish farms, as it can cause antibiotic resistance, which can be a serious threat to the health of end consumers. The nephrite administration used in the nephrite distillate showed a reduction of 10% to 40% of normal bacteria in seawater, as shown in the above experimental results. It would suggest that it is worth it. In particular, the jade tile of the product can be used as a coating material on the inner surface of the fish farm as it is, you can try the initial survival test of the fry in the tank of at least 2m in width, 3m in depth 1m.

Experimental Example 12

The next experiment was to use a necklace made from purgatory mined from the Chuncheon Purgatory Mine where the corn was harvested and oxol tea for healthy men and women around 39 years old and women around 19 years old. Was measured with a wave measurement analyzer (RADIONIC BIOFIELD ANALYZER) (trade name OMNI-SENSE) and confirmed the change in biorhythm. The results were as shown in Tables 18 and 19.

<Jade necklace (big one)> <Jade man (40 years old) Woman (19 years old) Before measurement after measurement Before measurement after measurement Angina (50121) 43 43 45 Diabetes (10932) 35 37 54 Hypertension (45031) 44 45 44 Sciatic nerve (42812) 24 53 54

* () Is a unique measurement code number of wave measurement analyzer (Omni sense).

<Jade snow tea> Man (39 years old) Woman (21 years old) Before measurement after measurement Before measurement after measurement Angina (52071) 53 64 35 44 Diabetes (11009) 47 53 42 43 Hypertension (40520) 48 57 33 41 Sciatic nerve (40228) 43 61 23 52

* () Is a unique measurement code number of wave measurement analyzer.

As can be seen from the above measurement results, the measured values from the wave measurement analyzer before and after using jade showed tremendous changes. The higher numbers indicate that the human body is good for jade. In the experimental example, healthy people were targeted, but it is expected that a higher value change can be obtained by measuring the patient. The wave measurement analyzer used in the above experiment was made by fusing the structure and digital technology of the traditional analytical lazionics. The analyzer has a pocket comb (64 kb). There are approximately 400 items of rate that can be measured for hormonal balance, metabolic disorders, vitamin deficiency, mineral deficiency, consciousness and meridians.

Reference values and measurement conditions of the wave measurement analyzer are as follows.

<Reference value of wave measurement analyzer> 0-25% Bad 25 to 45% good 45 to 55% Very good 55 to 75% good 75 ~ 100% Bad

<Error range of wave measurement analyzer>

± 5% margin of error depending on the environment of the measurement location and the personal condition of the operator.

<Measurement range of the wave measurement analyzer>

The measuring range is from -100 to 100% but mainly from 0 to 100%.

<Measurement environment>

It is the best place where there are few electromagnetic wave generation factors and there is no interference from other strong waves.

<Power supply>

Use both 220V AC adapter and built-in battery type (A4 X 4)

These experimental results suggest that the nephrite distillate of the present invention when used for drinking or medical use has a medical characteristic that promotes the treatment and metabolism of the human body.

As described above, according to the present invention, it is possible to obtain a purgatory distillate (extract material) from the purgatory ore collected from the purgatory mine, thereby preventing the human body's ability to treat diseases (headache, insomnia, indigestion, hand-to-hand pain, etc.). ), And it can be used actively to have medical characteristics such as growth and growth ability of animals and plants.

Claims (4)

Precipitated ore and pre-purified purified water through a pretreatment process such as crushing, sorting and washing are put into an evaporator at a ratio of 1: 4 and heated at a high temperature of 100 ° C. or higher, and the ore-containing substance eluted in purified water is heated with purified water. Jade extract, which is allowed to evaporate with water vapor and containing the ore-containing material is converted to distillate through heat exchange. According to claim 1, wherein the jade ore is defined as butch δ ¹⁸ 0 as a hollowstone jadeite in the high quality surrogate rock, and the composition [Semi-quantitative analysis of the sample (Semi -Quantitative Analysis (%)]. Silicon 34 Magnesium 10 Calcium 4.9 Iron 0.23 Aluminum 0.16 Copper 0.17 Cobalt 0.046 Manganese 0.14 Tin 0.024 Beryllium 0.00072 Is 0.0013 Titanium 0.0038 Nickel 0.0028 Chromium 0.0030 Miscellaneous 0 Pretreatment step of crushing, sorting and washing the mined ore to a certain size; Preparing purified water; Putting the pretreated ore and purified water in an evaporator in a ratio of 1: 4 and then heating it at a high temperature of 100 ° C. or higher so that the ore-containing material eluted in the purified water is evaporated together with the purified water; And Method of preparing a jade extract comprising the step of converting the water vapor containing the ore-containing material generated in the evaporation step into a distillate through heat exchange. According to claim 1, wherein the jade ore is defined as butch δ ¹⁸ 0 as a hollowstone jadeite in the high quality surrogate rock, and the composition [Semi-quantitative analysis of the sample (Semi -Quantitative Analysis (%)]. Silicon 34 Magnesium 10 Calcium 4.9 Iron 0.23 Aluminum 0.16 Copper 0.17 Cobalt 0.046 Manganese 0.14 Tin 0.024 Beryllium 0.00072 Is 0.0013 Titanium 0.0038 Nickel 0.0028 Chromium 0.0030 Miscellaneous 0