KR20010007650A - Preparation for betterment for changes of blood lipids containing Chlorella - Google Patents

Abstract

PURPOSE: A blood lipid improver containing Chlorella ellipsoidea is provided, which improves a blood lipid change and arteriosclerosis caused by a cadmium. CONSTITUTION: When a cadmium is administered to a monkey, a rabbit, a mouse for a long time, the amount of high density lipo-protein(HDL), cholesterol, triglyceride in blood serum are lowered. Chlorella ellipsoidea increases the total density of cholesterol, HDL-cholesterol, lowers AI and consequently represses an arteriosclerosis caused by a cadmium which increases total cholesterol, HDL-cholesterol, triglyceride. The blood lipid improver is characterized by containing an effective amount of chlorella wherein, the effective amount of chlorella is 1g/kg, a day, for an adult. The blood lipid improver can be manufactured in a form of tablet, injection, drink, tea.

Description

Preparation for betterment for changes of blood lipids containing Chlorella}

The present invention relates to a blood lipid improving agent containing chlorella (Chlorella ellipsoidea), and more particularly, to an improving agent capable of improving the change of blood lipid caused by cadmium by containing chlorella known as a health supplement. .

In this specification, several publications are cited by reference, which references only the author and year in the specification, the details of which are listed immediately before the claims.

Cadmium is a beautiful and corrosion-resistant metal with a polished surface, and is used in communication materials, alloys, solders, dental amalgams, and pigments. However, cadmium salt, cadmium vapor, and the like are poisonous and cause symptoms of poisoning in humans, and thus are disclosed as environmental pollutants.

In particular, cadmium lowers serum levels of high density lipoprotein (HDL), cholesterol, total cholesterol, and triglycerides when administered to monkeys, rabbits, and rats for a long time, causing atherosclerosis (Baranski). et al., 1983: Subramanyam et al., 1992: Skoczynska et al., 1994), causing glutamate oxaloacetic transaminase in the blood (weakly referred to as GOT) and glutamine pirbe It is known to increase the transaminase (weakly referred to as GPT) and to reduce the ratio of albumin and globulin (Katsuta et al., 1993).

Thus, efforts have been made to find improvers capable of ameliorating changes in blood lipids caused by cadmium, and the present invention has also been made as a result of these efforts.

In other words, the present inventors conducted a study to find an improvement agent that can improve the change of blood lipids caused by cadmium, and when administered chlorella known as a dietary supplement, triglycerides in the blood reduced by cadmium The present invention has been completed by discovering that the concentration of total cholesterol and HDL-cholesterol is increased and at the same time the indicators of liver disorders (GOT, GPT and albumin to globulin) are improved.

Accordingly, it is an object of the present invention to provide an improver capable of improving the change in blood lipids caused by cadmium.

In order to achieve the above object, the blood lipid improving agent according to the present invention is characterized by containing an effective amount of chlorella.

Hereinafter, the present invention will be described in more detail.

As can be seen in the nutrient components of Table 1, Chlorella ellipsoidea is a single-celled organism belonging to green algae grown in fresh water, and is characterized by strong photosynthetic ability, and in the chloroplast of Chlorella, Chlorella echis (Chlorella growth) Factors: C, G, F) are effective for the prevention, treatment and maintenance of health, and also rich in protein (60%), chlorophyll, minerals, vitamins, etc. To act.

Chlorella ellipsoidea nutrients nutrient Content ratio protein 53-63% Fat 7.5-12% Sugar 18-27% fibrin 2.5-11% Minerals (calcium, iron, etc.) 5 ~ 8% sign 1000-2000mg% vitamin A 100-300 mg% B ₁ 0.4-2.4 mg% B ₂ 2.1-5.8mg% B ₆ 0.9-2.3mg% B ₁₂ 0.2-0.4 mg% C 200-500mg% chlorophyll 3-4 mg%

In the present invention, the chlorella having the above-described efficacy increases the concentration of total cholesterol and HDL-cholesterol in the blood reduced by cadmium, thereby reducing the arteriosclerosis index, thereby suppressing the occurrence of arteriosclerosis, and preventing arteriosclerosis. I could find that.

In addition, since chlorella reduces the increase in blood GOT caused by cadmium, he discovered that hepatic injuries such as fatty liver can be suppressed.

Improving liver function disorders and changes in blood lipids containing chlorella of the present invention may be administered to the human body via an oral or parenteral route in an amount effective to improve liver function and an amount effective to improve changes in blood lipids. The liver function and blood lipid improving agent of the present invention can be made into a suitable dosage form according to the purpose of administration, route of administration, and the like, for example, as a pharmaceutical preparation such as tablets and injections, or in the form of a favorite food or health supplement such as tea or drink. It can also be formulated and drinkable. In the formulation, a pharmaceutically acceptable excipient that does not adversely affect the liver function and blood lipid improving effects of chlorella can be used. In either case, the dosage is preferably 60 kg of adult body weight and intake of about 60 g of chlorella per day. However, since the effect of functional foods and the like is preferable to take a small amount for a long time, rather than ingesting a large amount at a time, the dose or content of chlorella as a dietary supplement effect is less than 60g and administered for a long time, It is preferable to take.

Hereinafter, an Example is given and this invention is demonstrated concretely. However, the present invention is not limited to this embodiment.

<< Example 1 >>

1) Chlorella, reagents and animal feed

Chlorella ellipsoidea was supplied with dried centrifugal and hot air (80 ℃) for 3-4 days under freshwater, natural medium and natural light conditions in "Korea Chlorella Culture Center" (1394, Daman-ri, Jinrye-myeon, Gimhae-si, Gyeongsangnam-do). Cadmium (CdCl ₂ ) was used by Junsei (Japan). Reagents for the analysis of blood lipids GOT, GPT, albumin and globulin are Nissui Pharm. Co. Ltd. It was purchased from Tokyo (Japan). Animal feed was used as a standard solid feed (Samyang Feed Co., Ltd.) containing at least 21.1% protein, at least 3.5% fat, at least 5.0% fiber, at least 8.0% lean, at least 0.6% calcium and at least 0.4% phosphorus.

2) Experimental Animal Diet

Seven Sprague Dawley male rats (200 g) were used in one experimental group, and the control group was treated with a standard solid feed and drinking water, and the cadmium alone diet was made with 40 ppm of standard solid feed and cadmium, and the mixed diet of cadmium and chlorella. The group fed 40 days of standard solid feed mixed with crushed chlorella (1% of total diet) and drinking water added with 40 ppm of cadmium. Dietary intake and body weight were measured once a week under certain conditions.

3) Separation of Serum

The rats of each group fed for 40 days were fasted for 24 hours, anesthetized with ethyl ether, collected from the heart with a syringe, centrifuged at 3,000 rpm for 10 minutes, and serum was separated.

4) Analysis of Lipids, Enzymes and Proteins in Serum

The concentrations of triglyceride (TG), total cholesterol and HDL-cholesterol, GOT, GPT, total protein, albumin and globulin in serum were analyzed by Nissui Pharm. Co., Ltd. Tokyo, Japan) was measured by colorimetric method.

5) Results

① Intake of chlorella and cadmium

As can be seen in Table 2, the average amount of feed and cadmium intake of one rat was not significantly different between groups, and the average amount of chlorella intake of one rat was 221 mg. In addition, body weight was increased in the cadmium alone diet group than the control group or the mixed diet of cadmium and chlorella due to swelling of tissue due to cadmium toxicity (Edward and Reynolds, 1963).

Feed Intake (g / day) Chlorella intake (mg / day) Cadmium Intake (ppm / day) Weight (g) Control 24.3 - - 162.5 ± 34.8 Cadmium alone 22.1 - 24.3 202.0 ± 29.3 Cadmium Chlorella Blend Diet 22.1 221 25.0 175.0 ± 26.0

② Effect on Serum Cholesterol

As can be seen in Table 3 below, serum total cholesterol and HDL-cholesterol decreased with a significance of P <0.01 in the cadmium alone diet group than in the control group. This is consistent with Skoczynska's (1994) report that cadmium decreases the levels of total cholesterol and HDL-cholesterol in the blood, causing atherosclerosis.

TG (mg / dl) Total cholesterol (mg / dl) HDL-cholesterol (mg / dl) TG / HDL Cholesterol Atherogenic Index Control 80.7 ± 8.3 51.1 ± 7.3 27.9 ± 1.3 2.8 0.83 Cadmium alone 49.0 ± 3.0 ^* 36.9 ± 0.4 ^* 12.4 ± 1.6 ^* 4.0 1.97 Cadmium Chlorella Blend Diet 59.0 ± 7.3 ^** 41.1 ± 9.4 ^** 17.6 ± 0.2 ^** 3.4 1.33 * Decreased with significance of P <0.01 than that of the control group. ** Decreased with significance of P <0.05 than that of the cadmium alone diet group.

However, in the mixed diet of cadmium and chlorella (CdCl ₂ + chlorella), total cholesterol and HDL-cholesterol concentrations were significantly increased than in the cadmium alone diet. In this case, the increase in total cholesterol (average 4.2 mg / dl) corresponds to the increase in HDL-cholesterol, and the increase in HDL-cholesterol in the mixed diet group of cadmium and chlorella means that high density lipoprotein (HDL) is increased.

On the other hand, promoting the development of arteriosclerosis in the lipoproteins in the blood is a low density lipoprotein (hereinafter referred to as 'VLDL') is caused by the decomposition of very low density lipoprotein (hereinafter referred to as 'VLDL') Although HDL is one of its functions to remove unnecessary apoproteins contained in VLDL and LDL (Arai, 1991), HDL (HDL-cholesterol) ) Increases in blood, inhibiting arteriosclerosis (美濃, 1993).

Therefore, in view of the above results, since chlorella raises blood levels of total cholesterol and HDL-cholesterol due to cadmium toxicity and reduces arteriosclerosis index, the occurrence of arteriosclerosis can be suppressed.

③ Serum TG and HDL-Cholesterol

Serum TG is a major cause of atherosclerosis, and as the ratio of TG / HDL-cholesterol increases, the risk of atherosclerosis increases (Lands, 1986: Lee and Karel, 1990: 河 合 et al., 1989). It is also known that prolonged administration of cadmium to rabbits reduces serum TG and induces atherosclerosis (Subramanyam et al., 1992). This fact can be demonstrated from the result that the TG / HDL-cholesterol ratio of the serum of Table 3 above was increased in the cadmium alone diet group than in the control group.

On the other hand, the concentration of blood TG in the cadmium and chlorella diet group increased by an average of 10 mg / dl more than in the cadmium alone diet group. This means that Chlorella releases TG into the blood to maintain lipid metabolism. Conversely, the lower concentration of TG in the cadmium diet group was compared to the control group, suggesting that TG was not released into the blood in the form of VLDL by the hepatotoxicity of cadmium. This may be indirectly considered that apoprotein, a transporter of VLDL, causes severe liver failure due to cadmium alone diet, which inhibits protein synthesis, resulting in lower blood protein concentration than the control group (Table 5).

Therefore, dieting chlorella can increase the concentration of TG and HDL-cholesterol reduced by cadmium and at the same time reduce the ratio of TG / HDL-cholesterol, thereby preventing atherosclerosis.

④ Effect on serum enzyme activity in cadmium-induced hepatotoxicity

Administration of cadmium to rats results in necrosis of hepatocytes, increasing the activity of GOT and GPT that regulate transamimination of amino acids (Katsuta et al., 1993). This means that GOT and GPT are released from the liver into the blood by liver damage, especially in liver cirrhosis and fatty liver, its causative pathology, which significantly increases blood GOT and does not increase blood GPT (Usi). et al., 1992: Asda, 1958: Ghoshal et al., 1969).

As can be seen in Table 4 below, in the cadmium diet group, serum GOT increased about twice as compared to that in the control group. From these results, it is thought that dietary chlorella can suppress liver damage such as cirrhosis induced by cadmium.

On the other hand, GPT was not significant in the cadmium group compared to the control group. This is consistent with reports that in liver diseases such as acute hepatitis, GOT and GPT in blood are significantly increased during chronic liver disorders such as fatty liver and cirrhosis, and that GPT does not increase.

If cadmium causes fatty liver, the causative pathology of cirrhosis, to accumulate TG in liver tissue (Dominique et al., 1991), blood TG will decrease. Reflecting this, preglycerides in the blood by cadmium alone diet were about two times lower than those in the control group, and in the mixed diet of cadmium and chlorella, the TG concentration (49.0 ± 3.0 mg / dl) was increased in the chlorella alone diet group. To 59.0 ± 7.3 mg / dl (Table 3). This result indirectly proves that chlorella inhibits hepatic injuries such as fatty liver caused by cadmium, which is the same as the result of chlorella's reduction of blood GOT by cadmium (Table 4).

GOT (KU / mL) GPT (KU / mL) Control 76 ± 7.0 84.2 ± 8.2 Cadmium alone 166.6 ± 12.5 ^* 85.3 ± 3.8 Cadmium Chlorella Blend Diet 68.3 ± 10.0 71.8 ± 8.4 ^** * Decreased with significance of P <0.01 than that of the control group. ** Decreased with significance of P <0.05 than that of the cadmium alone diet group.

⑤ Effects on Serum Proteins in Cadmium-Induced Hepatotoxicity

In severe hepatic injuries such as cirrhosis and fatty liver, the serum total protein and albumin concentrations are lowered, and the ratio of globulin (G) to albumin (A) (A / G ratio) is lowered (Usi et al., 1992). . In addition, cadmium causes injuries to various organs such as liver, kidney, and lungs (Lars et al., 1992), and in particular, decreases in albumin synthesis in the liver due to hepatic injuries, resulting in a decrease in blood albumin and increased globulin Pathological phenomena caused by a decrease in A / G ratios vary from severe liver injury, diabetes, tumors, leukemia, and prostate cancer (Usi et al., 1992). Decrease (Katsuta et al., 1993).

That is, as can be seen in Table 5, the total protein and albumin concentrations in the cadmium diet group was lower than in the control group, and the A / G ratio was also reduced. This means that cadmium caused severe liver failure.

However, in the mixed diet of cadmium and chlorella, total protein, albumin concentration and A / G ratio were significantly increased than in the cadmium alone group.

Total protein (mg / dl) Albumin (mg / dl) Globulin ^*** (mg / dl) A / G ratio Control 87.1 ± 6.8 53.3 ± 4.2 33.8 1.6 Cadmium alone 77.4 ± 1.4 ^* 42.2 ± 2.7 ^* 35.2 1.2 Cadmium Chlorella Blend Diet 83.7 ± 4.2 ^** 53.0 ± 1.5 ^** 30.7 1.7 * Reduced with a significance of P <0.01 than in the control group. ** Reduced with a significance of P <0.05 than in the cadmium alone diet group. **** Globlin: Average total protein-average albumin

The above results indicate that chlorella inhibits hepatic impairment by cadmium, controls the synthesis of protein in the liver, and maintains total protein and albumin levels in the blood at the control level.

As described above, chlorella increases the concentrations of total cholesterol, HDL-cholesterol and TG, which are reduced by cadmium, which not only inhibits arteriosclerosis induced by cadmium, but also inhibits severe liver damage caused by cadmium. It can be seen.

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Claims (2)

An agent for improving lipid change in blood, comprising an effective amount of chlorella. According to claim 1, wherein the effective amount of chlorella is an improvement agent, characterized in that 1g per 1kg body weight per day based on an adult.