KR20010001813A - Continuous Process for the Production of Biosurfactant using a Technology of Immobilized Enzyme - Google Patents

Abstract

PURPOSE: A continuous production process of bio-surfactant that has a biocompatibility using enzyme immobilizing technique is provided, which cuts off the production cost by not involving additional separation process and reduces environmental pollution by using affinitive material for environment. CONSTITUTION: A continuous production process of bio-surfactant using enzyme immobilizing technique is characterized by comprising the following steps of: (i)manufacturing organic/inorganic mixed membrane where enzyme is immobilized, by sol-gel process; (ii)producing pure malto-oligosaccharide from starch by using a membrane reactor; (iii)immobilizing enzyme physically while agitating enzyme with pre-polymer when manufacturing poly-urethane foam and packing poly-urethane foam at PFR reactor; (iv)supplying malto-oligosaccharide to packed PFR reactor and producing bio-surfactant.

Description

Continuous Process for the Production of Biosurfactant using a Technology of Immobilized Enzyme}

Biosurfactants are produced in batch reactors using reducing sugars as the main raw material. In addition, we are studying a process to recycle yeast by ultrafiltration after supplying raw materials to a continuous production reactor and fermenting the reactor. All of these processes, however, contain additional separation processes, which contribute to an increase in production costs. The present invention is an invention of a process for producing a biosurfactant by an immobilized enzyme without any other separation function and at the same time performing the separation function.

The present invention is a production process that does not contain secondary environmental pollution and does not contain an additional separation process from the production of reducing sugars, the main raw material of the biosurfactant to the process of producing the biosurfactant. To achieve this.

First, in the process of producing reducing sugars from starch, the production of a membrane reactor capable of performing the reactor function and the separation function at the same time and the production of the membrane used in the membrane reactor. In the preparation of the membrane, an organic / inorganic hybrid membrane in which an enzyme is immobilized was prepared by using a sol-gel method of preparing glass or ceramic at low temperature, and the membrane was applied to a membrane reactor to prepare a process of producing reducing sugar from starch.

Second, it has mechanical properties and stability, it is easy to transport oxygen, easy to separate process and reusable, so that the enzyme is physically immobilized on the polyurethane foam that can be applied to the continuous process, and the polyurethane foam on which the enzyme is immobilized is tubed. The reactor was charged and applied to the PFR reactor. In addition, all of the materials used are environmentally friendly materials to establish a biosurfactant production reaction process without pollution.

Using enzyme fixation technology, we will manufacture environmentally friendly biosurfactant production process as shown in the figure below.

Example 1

Preparation of Organic / Inorganic Hybrid Membranes with Enzyme Immobilization and Application of Membrane Reactor.

The enzyme-immobilized membrane prepared by the sol-gel process was cut into 1 cm 2, and the enzyme activity was measured. As shown in Figure 2, it was confirmed that the enzyme remained stable for a long time. The membrane was used to make a membrane reactor (Figure 2), then starch was fed to the membrane reactor and the product produced was analyzed by HPLC. The analysis results by HPLC are shown in Figure 4, it can be seen that only one component of reducing sugar is produced more than 96% of the total product.

Example 2

Enzyme-immobilized Polyurethane Foam Production and Reactor Production

Polyurethane foams were synthesized using various polyols as prepolymers that are precursors of polyurethane foams. Polyurethane foams having various physical properties were prepared using the synthesized prepolymer, and FIG. 4 shows the water absorption of polyurethane foam over time.

As shown in Fig. 5, the enzyme was physically immobilized by selecting an optimal condition having excellent water absorption and physical properties, and the enzyme activity was measured. As shown in the figure, it can be seen that the enzyme activity is maintained for a long time.

The enzyme is immobilized on the polyurethane foam having the optimal physical properties, and the enzyme-immobilized polyurethane foam is filled in the PFR reactor, and only the maltooligosaccharides produced in the primary membrane reactor are supplied to provide biocompatible biosurfactants. Produced.

The surface properties and biocompatibility of the resulting surfactants were measured. The concentration and surface tension are shown in Fig. 7, and 33 dyne / cm at 14 mmol was obtained. Biocompatibility was confirmed by applying the generated surfactant to the arm for the presence of side effects, no other side effects for more than 24 hours, was able to synthesize a high quality biocompatible material.

By controlling the enzymes used in the membrane reactor, the production of high value-added materials from low value-added materials can be produced at low cost, and all biocompatible materials can be used to reduce environmental pollution. There is a number. In addition, the produced biosurfactants are biocompatible and have high added value, and can solve the problem of secondary contamination caused by synthetic surfactants.

Claims (2)

Preparation of Enzyme Immobilized Membranes by Sol-Gel Process and Preparation of Membrane Reactor. A process for producing maltooligosaccharide production of monocomponent and biosurfactant using the membrane reactor prepared in claim 1.