KR20000021322A - Stress releasing drink containing rose extract - Google Patents

Abstract

PURPOSE: A stress releasing drink containing rose extract which can reduce MHPG-SO4 and corticosterone which are increased by physical and psychological stress is provided by inhibiting the formation of activated oxygens effectively, such as super oxide radical or hydroxy radical which stimulate aging. CONSTITUTION: A stress releasing drink is comprised of 0.1% or 0.5% of rose concentrates prepared by condensing a rose fruit extract in vacuum condition, extracted with ethanol/1,3-butyleneglycol/purified water in the ratio of 7/1/3(v/v/v) added with each extract of soybean germ, bamboo shoots and oak mushroom in 0.05 hundred parts by weight(pts.wt.), respectively, 0.03pts.wt. of ginger extract, asparagine, arginine, taurine, calcium lactate and sodium benzoate in 0.05 pts.wt., respectively, vitamin C, B1 and B6 in 0.03pts.wt., respectively, citric acid in 0.07pts.wt., sorbitol in 0.5pts.wt., potassium iodide in 0.0002pts.wt., sugar in 2.5pts.wt., fructose in 4.5pts.wt., and 5.0% of grape extract. The drink is produced by mixing the ingredients, centrifuging the mixture at 1000 multiply with gram for 10 minutes, and collecting the supernatant to make a stress releasing drink. By administering a drink containing 0/1% of the rose extracts, MHPG-SO4 and corticosterone were reduced by 17% and 25%, respectively, thereby the result demonstrates the ability of the drink to release the socio-psychological stress.

Description

Stress Relief Drink Using Rose Extract

The present invention relates to a stress relief drink using a rose extract.

The biggest problem in the development of stress relief beverages in the past is how animal experiments can cause sociopsychological stress. A communication box, which can be solved through animal experiments, was developed in 1992 by Prof. Masahiro Ishikawa, Ehime University School of Medicine. The device (64 × 64 × 40 cm) consists of eight rooms with foot-shock stress and eight rooms with no socio-psychological stress, with regular installations of copper wires of diameter 0.5 cm at 1.3 cm intervals. It is.

Put the lab rat in the foot-shock stress and the sociopsychological stress, and then switch on the electrical switch. Only the black part is subjected to 2 mA electric shock for 10 seconds on the four feet (foot-shock stress) and 120 seconds for electricity. Not flowing is repeated for 1 hour. At this time, the white rat is not directly subjected to electric shock, but the stress that is reported when the black rat is suffering from electrical shock is regarded as social and psychological stress. And the control just puts an hour in the white part. The experiment is most effective between 09:00 and 11:00 am.

Until now, stress had been known for physical, chemical, and mental stress, but did not consider the development of stress-relieving beverages because they were not so seriously thought of as stress. However, as stress has recently been found to be the cause of all illnesses, the need for stress relief has been emphasized, but the need for stress relief drinks has not been recognized because it was impossible to relieve stress through drinks.

But the question is: (1) How do you measure stress? It is very difficult to study the problem of human beings who have complicated social life and (2) how to load stress through animal experiments and (3) how to evaluate whether stress is solved. Until now, it has been focused on the fact that under stress, gastrointestinal disorders occur.

The present invention has developed a functional stress-relieving beverage, which is based on a rose fruit extract, to which food ingredients such as soybean germ and bamboo shoots are added and prepared. The present invention, unlike the conventionally focused on the gastrointestinal disorders by experimenting with stress through the above-described animal experiments to the socio- psychological stress that a person will receive, as an autonomic nervous system Physiological changes in the sympathetic nerve activity and endocrine system are the subjects of the main evaluation.

The present invention is described in detail as follows.

That is, the extract extracted with ethanol / 1,3-butylene glycol / purified water (7/1/3, v / v / v) of rose fruit was concentrated in 0.1% and 0.5% of the concentrated solution under reduced pressure, soybean germ, Add 0.05 parts by weight of bamboo shoots and shiitake mushroom extracts and 0.03 parts by weight of ginger extract, and again add 0.05 parts by weight of asparagine, arginine, taurine, calcium lactate and sodium benzoate, and add 0.03 parts of vitamin C, B ₁ and B ₆ . Add weight parts, 0.07 weight part citric acid, 0.5 weight part sorbitol, and 0.0002 weight part potassium iodide. 2.5 parts by weight of white sugar and 4.5 parts by weight of fructose were added and mixed, followed by adding and mixing 5.0% of grape juice and centrifuging at 1,000 × g for 10 minutes to prepare a total 120 ml using supernatant to obtain functional stress of the present invention. Get a drink.

In regard to the stress-relieving beverage of the present invention, how it is effective to measure the psychosocial stress that a person will receive through animal experiments, and how to measure what is effective as an experimental method to relieve stress when the developed beverage is administered. As a result of various examinations, the following method was used to evaluate the effect of the stress relief drink of the present invention.

In other words, it is a social and psychological stress tester developed by Masahiro Ishikawa, a professor of medicine at Ehime University, and noradrenaline (noradrenaline), which is known to reduce stress caused by physical and psychological stress while loading stress on experimental animals. NA) content and MHPG-SO ₄ (3-methoxy-4-hydroxyp henylethyl-eneglycol sulfate) and corticosterone, which are known to be increased by these stresses, were not significantly different from the control group. The assay evaluates the physiological effects of the stress relief drink of the present invention.

The content of the rose extract and food ingredients used in the present invention, and experimental materials, experimental methods and results are as follows.

1. Experimental Materials

(1) stress relief drink

The stress relief beverage of the present invention is a concentrated solution of a rose fruit extract extracted with ethanol / 1,3-butylene glycol / purified water (7/1/3, v / v / v) under reduced pressure and concentration of 0.1%. And 0.5% by weight of soybean embryos, bamboo shoots, shiitake mushroom extracts, and 0.053 parts by weight of ginger extract, respectively, and 0.5 parts by weight of asparagine, arginine, taurine, calcium lactate, and sodium benzoate, respectively. Add 0.03 parts by weight of vitamin C, B ₁ , B ₆ , 0.07 parts by weight of citric acid, 0.5 parts by weight of sorbitol, and 0.0002 parts by weight of potassium iodide. 2.5 parts by weight of white sugar and 4.5 parts by weight of fructose were added and mixed, 5.0% grape juice was added and mixed, and centrifuged at 1,000 × g for 10 minutes to prepare a total of 120 ml using the supernatant.

(2) Animal test for test of effect

The experimental animals used in this animal experiment were (1) 42-day administration experiment using Sprague-Dawley rats (rats: 120 ± 10g, ♂) of 4 weeks old for adult disease and anti-aging effect test. For the effect of relieving experiment, 4 weeks-old ICR mice (mouse: 25 ± 3g, ♂) were administered for 4 weeks, followed by 1 hour a day in a social and psychological stress box for 3 days. After inducing stress, an aliquot of blood and brain is used to measure corticosterone in plasma and noradrenaline (NA) MHPG-SO ₄ (3-methoxy-4-hydroxypheny lethyl -eneglycol sulfate) in brain fractions Was measured to evaluate the stress relief effect.

(3) Reagent

Noradrenaline (NA), MHPG-SO ₄ (3-methoxy-4-hydroxyphenyl ethyl -eneglycol sulfate), and cortosterone (corticosterone) for measuring and evaluating the stress relief effect as the reagents used in this experiment were produced by Sigma. A special reagent was used, and other analytical reagents used more than first grade reagent.

2. Experiment Method

(1) odor and separation of blood

The animals were anesthetized with ether, and blood was collected from the heart, left for 2 hours at low temperature, centrifuged at 700 × g for 10 minutes, and the supernatant was treated with 0.05 ml of blood per 1.0 ml of anticoagulant (sodium heparin). The bottle was placed in a complete blood cell count (green cross) and frozen and stored at −70 ° C. to be used for the analysis of corticosterone.

(2) fraction of brain tissue

Aliquot the brain tissue and place it in buffer (1.15% KCl / 10mM phosphate buffer + 5mM EDTA, pH 7.4) to freeze and store at -70 ℃. Before analyzing the stress relief effect, the contents of noradrenaline (NA) and MHPG-SO ₄ were analyzed according to the following pretreatment, respectively.

(3) measurement of corticosterone

Corticosterone content of plasma isolated from blood was measured according to the method of Sliber et al. (1958). After shaking 1.0 ml of plasma vigorously for 30 seconds in three times the amount of petroleum ether, the solvent layer is discarded after centrifugation. After diluting the purified plasma with 5.0 ml of distilled water, the mixture was extracted by shaking with 15.0 ml of methylene chloride for 30 seconds in a centrifuge tube. The extract is added to a tube containing 1.0 ml of 0.1N NaOH solution, shaken quickly and centrifuged.

After centrifugation, the alkali layer is discarded and 10.0 ml of methylene chloride is placed in a tube containing 2.0 ml of 30N sulfuric acid. After shaking for about 30 seconds, after centrifugation for 1 to 2 minutes, the upper solvent layer was discarded and the lower layer was 2.0 ml and left at room temperature for 30 to 90 minutes, and measured at excitation 470 nm and emission 530 nm. Corticosterone standard solution was dissolved in 5.0 ml of corticosterone in 5.0 ml of ethanol, diluted with 1,000 ml of distilled water, and then 0.1 to 0.5 ug. Was quantified.

(4) measurement of noradrenaline

The content of noradrenaline (NA) in the brain was measured according to Kohno's method. Brain tissue is ground in a low temperature room with 4.0 ml of 0.2 N sulfuric acid containing 0.1% sodium metabis sulfate (Na ₂ S ₂ O ₅ ), and then centrifuged at 8000 × g for 5 minutes. The supernatant is taken 2.0 ml each to simultaneously measure NA and MHPG-SO ₄ . Next, 3.0 ml of 0.4N PCA containing 0.1% Na ₂ S ₂ O ₅ and 0.05% EDTA was added. After centrifugation at 8000 × g for 10 minutes, the supernatant was transferred to a cap tube and stored at −45 ° C. until the experiment. The pyrex column (diameter 6 mm, height 25 cm) for NA measurement is filled with glass wool and filled with alumina activated at pH 7.5-8.0 at a height of exactly 6.0 cm. NA is extracted from the column by 0.05 ml PCN 2.0 ml. After extraction, the solution is converted to a fluorescent compound by 2.0 ml of iodine reagent. Fluorescence of NA was measured at excitation 380 nm and emission 495 nm. NA standards were prepared at a concentration of 100 ug / ml and the content of noradrenaline (ng / g brain) was quantified according to the above procedure by a standard calibration curve.

(5) Measurement of MHPG-SO ₄

The content of MHPG-SO ₄ in brain tissue was measured according to the method of Kohno (1979). Brain tissue is ground in a low temperature room with 4.0 ml of 0.2 N sulfuric acid containing 0.1% sodium metabis sulfate (Na ₂ S ₂ O ₅ ) and centrifuged at 8000 × g for 5 minutes. The supernatant is taken 2.0 ml each to simultaneously measure NA and MHPG-SO ₄ . Each sample is adjusted to pH 6.0-6.5 with 0.3 N barium hydroxide solution, and then all samples are made to the same amount with distilled water. After centrifugation at 8000 x g for 10 minutes, the supernatant was transferred to a cap tube and stored at -45 ° C until the experiment. The pyrex column (diameter 6 mm, height 25 cm) for the measurement of MHPG-SO ₄ is covered with glass wool and filled with DEAE sephadex A-25 at exactly 6.0 cm. MHPG-SO ₄ is extracted from the column as 2.0 ml 0.4N PCA with 0.1% sodium metabissulphate.

Immediately after the separation of MHPG-SO ₄ , 0.1 ml of 0.2% cysteine and 0.1 ml of 70% PCA are added, and these reaction mixtures are boiled in a water bath at 90-95 ° C. for 25 minutes. In this case, blank is added without heating. After adding 0.3 ml of ethylenediamine, all samples were placed in a hot bath for 5 minutes and cooled. Fluorescence of MHPG-SO ₄ was measured at excitation 325 nm and emission 465 nm. MHPG-SO ₄ standard solution (100ug / ml) was prepared with 0.5% sodium metabis sulfate (Na ₂ S ₂ O ₅ ) concentration and the content of MHPG-SO ₄ (ng / g brain) by the above procedure according to the standard calibration curve. Was quantified.

3. Evaluation of Experimental Results

(1) Reducing Effect of Corticosterone

Corticosterone, which is present in plasma, is a hormone involved in the metabolism of glucose in the adrenal cortex and is used as an indicator of stress. In other words, the physical stress (foot-shock stress) such as electrical shock not only increases significantly, but also sociopsychological stress (non-foot-shock stress) increases significantly under load Reducing the amount of corticosteroids can be a way to relieve stress.

As a stress-relieving beverage of the present invention, a rose fruit extract-added beverage was administered to an ICR mouse for 4 weeks, followed by an electric shock of 2 mA for 1 hour from 10:00 to 11:00 for 3 days (after 10 seconds of shock). 120 seconds rest) to see this while receiving a psychological stress, and compare the effect on the content of corticosterone (corticosterone) as the administration effect of chrysanthemum extract-added beverage is shown in Table 1.

☞ Significance test: compared to psychological stress group ^*** p <0.001

Rose extract-added as a stress-relieving beverage using the control group (26.03 ± 0.56 ug / dl plasma: 100%) as a control group of psychological stress under psychological stress under electric shock load (foot-shock stress) Comparing the administration groups of beverages, the 0.1% and 0.5% rose extract-added beverages were 19.70 ± 4.21ug / dl plasma and 16.14 ± 3.97ug / dl plasma, respectively, 75.7% and 62.0%, respectively, compared to the control group. It has been scientifically proven that the content of is effectively reduced stress by 24% and 38%, respectively.

(2) increase effect of noradrenaline

Noradrenaline (NA) is a hormone secreted with adrenaline and is secreted as a neurotransmitter at the sympathetic nerve endings of brain tissue. Among these neurotransmitters, noradrenaline is also used as an indicator of stress. In other words, not only is it significantly reduced under physical stress (foot-shock stress) load such as electric shock, but also significantly under sociopsychological stress (non foot-shock stress) load. Increasing the amount of adrenaline can be an effective way to relieve stress.

As a stress-relieving beverage of the present invention, a rose fruit extract-added beverage was administered to an ICR mouse for 4 weeks, followed by an electric shock of 2 mA for 1 hour from 10:00 to 11:00 for 3 days (after 10 seconds of shock). 120 seconds rest) to see this while receiving a psychological stress, and compare the effect on the content of noradrenaline (noradrenaline) as a dose effect of chrysanthemum extract-added beverages.

Rose extract developed as a stress-relieving beverage using the psychological stress group which received psychological stress under electric shock load (306.3 ± 21.7ng / g brain: 100%) as a control group When comparing the effect of the fruit extract) -added beverages, the 0.1% and 0.5% -rose extract-added beverages were 298.0 ± 65.1ng / g brain and 416.2 ± 64.3ng / g brain, respectively, 97.3% and As 135.9%, the 0.1% -rose extract-administered group did not recognize a significant effect, but the 0.5% -rose extract-administered group increased significantly by 36%, scientifically proved to be very effective in relieving stress.

☞ Significance test: compared to psychological stress group ^*** p <0.001

(3) reduction effect of MHPG-SO ₄

MHPG-SO ₄ (3-methoxy-4-hydroxyphenylethyleneglycol sulfate) is a metabolite of noradrenaline (NA), which is secreted as a neurotransmitter at the sympathetic nerve endings of brain tissue, and is used as an indicator of stress like NA. . This is because MHPG not only increases significantly during physical stress (foot-shock stress) load such as electric shock, but also significantly increases under sociopsychological stress (non foot-shock stress) load. Reducing the content of -SO ₄ may be an effective way to relieve stress.

As a stress-relieving beverage of the present invention, a rose fruit extract-added beverage was administered to an ICR mouse for 4 weeks, followed by an electric shock of 2 mA for 1 hour from 10:00 to 11:00 for 3 days (after 10 seconds of shock). 120 seconds rest) to see this, and to receive the psychological stress, and then compare the effect on the content of MHPG-SO ₄ as the administration effect of the rose extract-added beverage is shown in Table 3.

☞ Significance test: Compared to psychological stress group ^** p <0.01.

Rose extract-added as a stress-relieving beverage with the control group (163.2 ± 15.6ng / g brain: 100%) as the control group (163.2 ± 15.6ng / g brain: 100%) who reported the impact of foot-shock stress. Comparing the administration effect of the beverages, the 0.1% and 0.5% rose extract-added beverages were 136.3 ± 9.7 ng / g brain and 132.4 ± 14.8 ng / g brain, respectively, 83.5% and 81.1%, respectively, MHPG-SO ₄ It has been scientifically proven that the content of is significantly reduced by 17% and 20%, respectively, to effectively relieve stress.

In conclusion, the development of functional stress-relieving beverages prepared from 10 kinds of food extracts such as soybean germ and bamboo shoots in rose fruit extract is very effective. Using experiments, it was proved that the induction of social and psychological stress of people in complex social lives is also very effective.

In addition, as a result of the administration of the rose extract-added stress releasing beverage of the present invention, the content of noradrenaline, which is significantly reduced at physical and psychological stress loads, was effectively increased, while the corticose was significantly increased at physical and psychological stress loads. The contents of corticosterone and MHPG-SO ₄ were effectively reduced.

Example 1

0.5 parts by weight of rose fruit extract is mixed with 0.03 to 0.05 parts by weight of 8 food ingredients such as soybean germ and bamboo shoots, and then calcium lactate and sodium benzoate. 0.05 parts by weight, 0.0002 parts by weight of potassium iodide, 0.03 parts by weight of vitamins C and B ₁ , 0.07 parts by weight of citric acid and 0.5 parts by weight of sorbitol are added. 2.5 parts by weight of white sugar and 4.5 parts by weight of fructose were added and mixed, followed by adding and mixing 5.0% of grape juice, centrifuging at 1,000 × g for 10 minutes to remove the precipitate, and preparing the whole to be 120 ml using a supernatant solution. The stress relief drink of this invention is obtained.

Stress-relieving beverage of the present invention was analyzed by analyzing the effect on the content of corticosterone in plasma as a stress indicator component by using a social and psychological stress load device, 0.1% and 0.5% -rose The extract-added beverages were 19.70 ± 4.21ug / dl plasma and 16.14 ± 3.97ug / dl plasma, respectively, 75.7% and 62.0%, respectively, with 24% and 38% decrease in corticosteroid content, respectively. Scientifically proven to relieve stress.

In addition, the analysis of the effect on the content of noradrenaline in the brain cells, 0.1% and 0.5% rose extract-added beverages were 298.0 ± 65.1ng / g brain and 416.2 ± 64.3 ng / g brain, respectively 97.3% and 135.9%, respectively, of the control group showed no significant increase in 0.1% -rose extract-added beverage, but 0.5% -rose extract-added beverage increased 36% significantly. Comparing the effects of developmental stress relief beverages on the content of MHPG-SO ₄ as a metabolite, 0.1% and 0.5% -rose extract-added beverages were 136.3 ± 9.7ng / g brain and 132.4 ± 14.8ng / g, respectively. As brain, 83.5% and 81.1% of MHPG-SO ₄ were significantly reduced by 17% and 20%, respectively.

Claims (1)

Rose extract is mainly composed of 0.01-10.0 parts by weight of chrysanthemum extract, 0.01-5.0 parts by weight of soybean embryos, bamboo shoots and shiitake mushroom extracts, 0.01-5.0 parts by weight of ginger extract, 0.01-5.0 parts by weight of ginger extract, asparagine, arginine, taurine, 0.01 to 5.0 parts by weight of calcium lactate and sodium benzoate, 0.0001 to 0.05 parts by weight of potassium iodide, 0.01 to 5.0 parts by weight of vitamins C, B ₁ and B ₆ , 0.01 to 5.0 parts by weight of citric acid and 0.1 to 5.0 parts by weight of sorbitol Add and mix 2.5 to 30.0 parts by weight of white sugar and 0.5 to 10.0 parts by weight of fructose, add and mix 0.5 to 20.0 parts by weight of grape juice, centrifuge at 1,000 x g for 10 minutes to remove the precipitate, and remove the supernatant. Stress relief drink using a rose extract, characterized in that manufactured.