KR20000017719A - anti-cancerous bamboo salt - Google Patents

Abstract

PURPOSE: An anticancer bamboo salt with relatively-rich potassium content in comparison with sodium content of general salts is provided which shows excellent anticancer effect in that its has no peroxidative facilitating effectiveness and low reinforcing mutation effect by a carcinogen. CONSTITUTION: The overall process is followed by the next steps of: (1) Finding out inorganic contents for processed salt including bamboo salt, mechanic salt and sun-dried salt respectively; (2) Measuring peroxidative numerical values for the salts to examine mechanism to reinforcing carcinogenic effect; (3) and Examining reinforcing mutation effect of the salts by Ames' mutation induction test and SOS chromotest.

Description

Anti-cancerous bamboo salt

The present invention relates to anticancer bambooitis. More specifically, the present invention relates to an anticancer bamboo salt having a high K content, which does not promote peroxidation and exhibits an antimutagenic effect on carcinogens.

Salt is the most commonly consumed seasoning used to cook almost any food, affecting shelf life and flavor, or as a table salt. There are various kinds of salts. According to KS standard, if you use the specification of refined salt, salt is divided into natural salt and refined salt, and refined salt is classified into mechanical salt and processed salt. Solar salt is a salt crystal obtained by collecting seawater from the west coast and evaporating moisture by solar heat and wind. Recently, sea salt (fresh salt) comes out of sea water and bitter salt water, which means that the sea salt on the west coast is crushed, washed with water to remove impurities and water, and then centrifuged. On the other hand, highly purified mechanical salts are mechanically mass-produced with salt (reagent NaCl and Hanjugeum) extracted with sodium chloride only by using seawater and using ion exchange membranes. In recent years, processed salts commonly used in the market are commonly processed by heating process. Natural salts are baked twice in a ceramic reactor at a high temperature of 800 ° C. or higher to remove impurities, water, and harmful components (baked salt) and higher temperatures. It is baked three times at high temperature over 1300 ℃. Bamboo salt has been manufactured in Korean temples, etc. Chopped sun-dried salt into bamboo, and the bamboo inlet was kneaded and sealed in mud and heated to 1000 ~ 1300 ℃ 8 times in a kiln, and then sprinkled with ninth pine nuts on firewood 1300 ~ 1700 ℃ When heated, the salt melts and cools to form bamboo salt crystals.

Such salts are one of the important minerals that keep the nerves and muscles excited while physiologically balancing acid and alkali. However, it is known that ingestion of salty foods and salted foods may cause gastric cancer and stroke, and salt is known to help cancer development. The mechanism behind the role of NaCl in carcinogenesis is that NCl acts as a highly permeable surfactant and is a mediator of carcinogens, resulting in N-methyl-N'-nitro-N-nitrosoguanidine (N-methyl-N'-nitro). -N-nitrosoguanidine;

Carcinogens such as MNNG) increase the penetration into mucous membranes and increase the effective concentration to target cells. In addition, NaCl is involved in cell proliferation, and the mechanism of cell proliferation is suggested. First, cell proliferation is promoted by cytotoxicity of NaCl. High concentration of NaCl destroys gastric mucosa of gastric mucosa uniformly. Prolongs damage to cells to multiply. Second, salt affects lipid peroxidation in gastric mucosa and is involved in cell proliferation. After 5 weeks of dietary supplementation with 2-4% NaCl, mucosal cells proliferated as lipid peroxidation was increased in the stomach, and Takahashi et al. Reported that NaCl induced lipid peroxidation in target tissues in gastric mucosa and urine. Malondialdehyde (MDA) was reported to increase with increasing NaCl concentration. And thirdly, salt increases ODC (ornithine decarboxylase) activity. High concentrations of NaCl are known to increase ODC activity of gastrointestinal mucosa, resulting in increased DNA synthesis.

On the other hand, the oxidation-promoting effect of NaCl in food promotes lipid oxidation when added to salt-free meat, causing loss of flavor and color. However, Rhee et al. Reported that the substitution of NaCl with KCl reduced the promotion of lipid oxidation by salts. NaCl and MgCl ₂ rancid both raw and cooked samples, whereas KCl increased rancid only in raw meat. Therefore, replacing NaCl with KCl was the most effective in reducing the rancidity of the sample. Watts and Peng have the effect of promoting rancidity in pork that was not heated during the freezing of NaCl, MgCl ₂ , Na ₂ NO ₃ , NaCH ₃ COO, and K ₂ NO ₃ , but KCl had no such effect and Zipser et al. Reported to be somewhat inhibited. Therefore, NaCl in salt has the strongest peroxidation promoting effect and KCl is shown to have little such effect.

Jacobs induced tumor formation in rat small intestine by DMH (1,2-dimethylhydrazine) due to KCl (0.5%) supplementation, resulting in tumor formation from 40% to 5% when treated with KCl (p <0.05). The concentration of K supplementation was not toxic at all. Thus K exhibits anticancer effect.

Therefore, the inventors of the present invention examined the mineral content of processed salts including bamboo salt, and mechanical salts and sun salt, and found that mechanical salts had higher Na content than other salts and contained almost no minerals such as K, Mg, and Ca. Based on the results of the study, bamboo salts containing a large amount of K have lower peroxide-promoting ability than natural salts and mechanical salts of which Na is the main ingredient, and Salmonella typhimurium TA100 strain and carcinogen MNNG Significantly lowered the mutagenicity was confirmed that there is an excellent effect showing anticancer properties and completed the present invention.

Therefore, an object of the present invention is to provide a bamboo salt exhibiting anti-cancer properties due to the ability to inhibit peroxidation and to inhibit mutations caused by carcinogens.

The object of the present invention is to examine the mineral content of the salt and the salts of the processed salt, including the bamboo salt and the mechanical salt and sun salt, and then to investigate the mechanism of the salt-to-carcinogenic effect of the salt sample and the mutagenicity test of Ames and SOS chromo A test (SOS chromotest) was also achieved by investigating the mutagenic effect of the salts.

Hereinafter, the configuration and operation of the present invention.

1 is a graph showing the effect of NaCl on the autoxidation of linoleic acid.

Figure 2 is a mutation of the Salmonella typhimurium TA100 strain (N-methyle-N'-nitro-N-nitrosoguanidine; MNNG) strain N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) It is a graph showing the effect of various salts on induction capacity.

The present invention comprises the steps of measuring the mineral content of each of the processed salts, including mechanical salts, sun salts and bamboo salts in oil and fat using an Inductively Coupled Plasma Atomic Emission Spectrumeter: ThermoJarrell Ash ICP-AES-IRIA.USA; After adding each salt sample to fats and oils and autooxidizing, adding acetic acid to the extract extracted with water and chloroform, shaking with adding saturated KI solution, and titrating with 0.01 N sodium thiosulfate using starch solution as an indicator to obtain peroxide value. ; Inoculate S9 mixture of microsomal enzyme compounds of rat liver with processed strains including strains, mechanical salts, sun salts and bamboo salts, and mutagen MNNG. Incubate with a minimum glucose agar plate and incubate again. Investigating the effects of mutations; After incubating and diluting the E. coli PQ37 / plasmid 101 (PQ37) microbial solution, the fungal diluent was inoculated into 96-well plates in which the processed salt and the mutagen, including the mechanical salt, sun salt and bamboo salt, were separately injected to induce the SOS reaction. It is composed of the step of investigating the effect of mutation by measuring the galactosidase (β-galactosicase) activity and alkaline phosphatase activity.

According to the above composition, the MDA content of urine is recognized as a direct measure of lipid peroxidation status in other organs or gastric mucosa in vivo. As NaCl increases lipid peroxidation in target tissue, the concentration of NaCl in gastric mucosa and urine increases. According to Takahashi M. et al., Report that an increase in malondialdehyde (MDA) and a diet containing 2 to 4% of NaCl for 5 weeks resulted in an increase in the proliferation of mucosal cells in parallel with the increase in lipid peroxidation. Since salt in the diet affects lipid peroxidation in gastric mucosa and is involved in cell proliferation, we compared salt peroxidation promoting effect as one method to estimate the mechanism of salt's carcinogenic effect.

Salt samples used in the present invention are two kinds of purified mechanical salts commercially available (NaCl reagent (Junsei Chemical Co., Ltd) and Hanjugeum)] and two kinds of natural salts [west coast sun salts purchased from Sansan Co., Ltd.] , Raw salt Co., Ltd.] and three kinds of processed salts (baked salt Co., Ltd.). Mountain salts, bamboo salt (hazelnut) were used, respectively.

Hereinafter, the specific method of the present invention will be described in detail with reference to Examples.

The scope of the present invention is not limited only to these examples.

Example 1 Measurement of Mineral Content in Salt Samples

2 kinds of mechanical salts [for NaCl reagent (Junsei Chemical Co., Ltd. and Hanjugeum)] and 2 kinds of sun salt [West coast salt, west salt purchased from Sansan Co., Ltd.] and 3 processed salts [ Baked salt Co. 1 g of each of the salts and the bamboo salt (harmonic porridge) were dissolved in 1% nitric acid solution and heated in a heating tube for 3 hours, followed by diluting Na by 1,200 times and other minerals by 600 times. The mineral content of the samples was measured using an Inductively Coupled Plasma Atomic Emission Spectrometer (Thermo Jarrell Ash ICP-AES-IRIS.USA). The Na content in the salt was measured by an atomic absorption spectrometer (Atomic Absorption Spectromerer; Thermo Jarrell Ash AA Spectrometer 1000/4000, USA), and the Cl was followed by the Mohr method, and the amount of NaCl was determined by titration using 0.1 N AgNO ₃ . As a result, as shown in Table 1, the reagent NaCl was 99.8%, and other inorganic elements such as Ca, K, and S were detected in a small amount, and the mechanical salt extracted was 99.8%. , Ge was somewhat higher than reagent NaCl. Specifically, the NaCl content of the mechanical salt was the same as that of the reagent NaCl sample (99.8%), but the Na content (33.6%) was lower than that of the reagent NaCl sample (39.4%). It seems to be because the method to convert to NaCl. In contrast to mechanical salts, natural salts had a lot of Ca (1,037ppm), K (3,701ppm), Mg (10,266ppm), and S (7,456ppm), but Mg (5,883ppm) of fresh salt which removed impurities and water from sea salt. Most minerals such as Ca (579 ppm), K (1,661 ppm), and S (4,708 ppm) were reduced by half. Gansu is a liquid that falls out with water when it is stored for a long time, and has a bitter taste. In general, it is used to remove the salt to improve the taste. In this experiment, the relative mineral content was measured by centrifugation of the salt of the sea salt to measure the components in the liver. As shown in Table 2, the water contained a lot of Mg (53,618ppm), and the water contained a large amount of K (10,030ppm), S (24,299ppm), Zn (9,332ppm) in addition to Mg. As was very low. A small amount of Pb, Al, Cr, and the like was detected as a heavy metal, and trace amounts of Co, Hg, Ni, Se, and the like were also detected. Baked salt had Ca (926ppm), K (2,729ppm) and Mg (8,750ppm), and Fe was 9.9ppm. The salts of Ca (1,952ppm), K (4,255ppm), and Mg (12,628ppm) were high, and Fe (12.7ppm) also showed that their concentrations were higher than that of roasted salt (Table 1). . In particular, bamboo salt was 11,136ppm, which was higher than natural salt or other processed salt.

Mineral content of various salts analyzed by ICP-AES Salt Type Mineral Refined salt Sun salt Mechanical salt NaCl (for reagents) One address Sun salt Fresh salt Baked Salt Salary Bamboo salt AS 6.0 1.4 ND ND ND ND ND Ca 36 161 1037 579 926 1952 390 Cu 4.5 1.2 4.1 3.2 1.5 2.1 6.8 K 21 870 3701 1662 2729 4255 11136 Mg ND 10 10266 5883 8750 12628 3552 Mn 0.1 0.1 4.5 2.1 3.4 3.1 4.1 P 1.3 0.3 89 66 82 100 809 Pb 5.3 7.7 11.7 ND 1.2 ND ND S 24 33 7459 4708 5385 7873 1525 Zn 9.3 2.8 4.3 4.6 1.6 3.0 3.1 Fe 1.4 0.4 2.6 0.6 9.9 12.7 99.5 Ge ND 93 78 23 14 ND 256 Si 9 4 22 9 258 423 478 Na (%) ¹⁾ 39.4 33.6 36.2 38.1 35.0 31.9 31.6 NaCl (%) ²⁾ 99.8 99.8 94.4 97.9 93.0 92.4 93.6 [Note] Unit: ppm ¹⁾ Na (%): Assay by AAS ²⁾ NaCl (%): Assay by titration with 0.1N AgNO ₃ ND: Not detected

Mineral Content of Grafts from Sea Salts Analyzed by ICP-AES mineral Content (ppm) mineral Content (ppm) AS ND Ge 37.8 Ca 193.0 Si 27.4 Cu 4.2 Al 1135.0 K 10030.0 Ba ND Mg 53618.0 Co 1.1 Mn 1.4 Cr 771.3 P ND Hg 22.8 Pb 280.8 Ni 3.2 S 24299.0 Se 2.3 Zn 9332.0 Na ¹⁾ 5.1 Fe 4.7 NaCl ²⁾ 29.8 ¹⁾ Na (%): analyzed by AAS ²⁾ NaCl (%): analyzed by 0.1N AgNO ₃ titration ND: not detected

Example 2 Peroxide Promoting Effect of Salt Samples by Peroxide Value Analysis

After dissolving 1.0 g of linoleic acid in 20 mL of ethanol, 25 mL of phosphate buffer (0.2M, pH 7.0) was added and mixed. And the salt sample was dissolved in phosphate buffer (phosphate buffer) to make the corresponding concentration and 1mL each was added. After 24 hours of automatic oxidation at 50 ° C., these reaction solutions were transferred to a separatory funnel, and then extracted three times using a small amount of water and 25 mL of chloroform, and the lower layers were collected. The mixture was extracted three times with 25 mL of acetic acid, and then the lower layer was collected, and 25 mL of acetic acid was added thereto. Then, 1 mL of saturated KI solution was added, shaken for 1 minute, and left for 10 minutes in the dark, 50 mL of distilled water was added as an indicator. POV was determined as follows by titration with 0.01 N sodium thiosulfate solution.

S: 0.01 N-Na ₂ S ₂ O ₃ titration (mL)

N: prescribed concentration of Na ₂ S ₂ O ₃

L: weight of fat or oil (g)

As a result, as shown in FIG. 1, the effect of salts involved in the automatic oxidation of linoleic acid by treating 0.2 to 2% of NaCl promoted peroxidation at 0.4%, the maximum at 0.6%, but at higher concentrations. Lowered again. Therefore, two mechanical salts (for NaCl reagent, mechanical salt) and two natural salts (west coast salt, fresh salt) and three processed salts (baked salt) at 0.4% concentration with a sharp increase in POV to see the relative difference in salt. , Livestock, bamboo salt) were used in the experiments to determine the peroxidation effect. As shown in Table 3, 632 ± 64 meq / kg in the salt-free treatment group was the lowest peroxidation effect was 727 ± 112 meq / kg, and there was no difference from the control group (p <0.05). Next, natural salt (764 ± 90meq / kg), fresh salt (767 ± 7meq / kg) without salt and raw salt (817 ± 75meq / kg) and fresh salt (823 ± 76meq / kg) were processed. Peroxidation promoting activity was higher than that of the control group (p <0.05). However, peroxidation effect was lower than that of mechanical salt (NaCl; 840 ± 15meq / kg Hanju Geum; 948 ± 38meq / kg). In this experiment, mechanical salts showed the highest peroxide-promoting capacity, and among them, Hangeum showed the highest promoting capacity (p <0.05).

Effect of Various Salt Samples on the Automatic Oxidation of Linoleic Acid incubated at 50 ° C for 24 Hours Sample (0.4%) POV (meq / kg) Control 632 ± 64 ^a NaCl 840 ± 15 ^bc Mechanical salt 948 ± 38 ^c Sun salt 764 ± 90 ^b Fresh salt 767 ± 7 ^b Baked Salt 817 ± 75 ^b Salary 823 ± 76 ^b Bamboo salt 727 ± 112 ^ab [Note] ^{a ~ c} : The meaning of the letters next to inserted data in the column indicates a significant difference of 0.05 level as measured by Duncan's multiple rangetest.

Example 3 Mutation Effect of Salt Samples

Among the chemicals used in this example, Aflatoxin B1 (AFB1) was purchased from Sigma, USA and the mutant N-methyl-N'-nitro-N-nitrosoguanidine (N-methyl-N'-nitro-N). -nitrosoguanidine (MNNG) was purchased from Aldrich, USA. AFB1 was dissolved in dimethyl sulfoxide (DMSO), and MNNG was dissolved in distilled water. O-nitrophenyl-D-galacto-pyranoside (ONPG), p-nitrophenyl posphate disodium (PNPP), phosphate buffered sardine saline; PBS) is manufactured by Sigma Chemical Co. (USA).

Hereinafter, the mutagenic effect will be described in detail in the following experimental example.

Experimental Example 1: Ames mutation induction test

In this experimental example, salts were classified by type, namely two kinds of mechanical salts (for NaCl reagent, mechanical salts (Hanjugeum)), two kinds of sun salts (west coast salt salts (natural salt), fresh salt), and three processed salts (baked salt, fresh salt, bamboo salt). Each was treated with 3-25%, and the mutagenicity test was performed in the Ames test system. Salmonella typhimurium TA100, a histidine nutrient composition of Salmonella typhimurium LT-2, used in this experiment was provided by Dr. BN Ames of the University of California, USA. It was. These experimental strains were used to identify genotypes such as histidine requirements, deep rough (rfa) mutations, uvrB mutations, and R factor when new frozen permanents were prepared. In order to activate the indirect mutagen (AFB1), a mixture of S9, a microsomal enzyme compound of liver, was prepared according to the method of Maron and Ames. A S9 mixture was prepared with a micromal enzyme fraction of livers induced by intraperitoneal injection of Aroclor 1254 5 days prior to dissection using about 200 g of male Sprague-Dawley rats. The concentrations of samples and mutagens used in mutation and mutagenic effects experiments were determined through preliminary experiments (dose response and toxicity experiments). The preincubation test used mainly in this experiment was 0.5 mL of S9 mix (for indirect mutations) or 0.5 mL of phosphate buffer (for direct mutations), strains cultured overnight (1 to 2 × 10 ⁹ cells / mL). A) 0.1 mL, a sample (50 μl) and a mutagen (50 μl) were put in a cap tube in an ice bath, lightly vortexed, and preincubated at 37 ° C. for 30 minutes. 2 mL of each top agar at 45 ° C. was poured into each tube, vortexed for 3 seconds, plated on a minimal glucose agar plate, incubated for 48 hours at 37 ° C., and the number of return mutations was counted. As a result, the mutagenicity was observed for MNNG in all treatment groups, but showed a slightly lower number of mutations in processed salts such as roasted salt, salt, and bamboo salt (Fig. 2). At 3% concentration, 1445 ± 136 NaCl for reagent, 1837 ± 694 mechanical salt (PS), 1404 ± 198 natural salt (CS), and 1224 ± 130 raw salt (SS), but the processed salt (GS) ), 938 ± 26 dogs, 948 ± 17 dogs (SK) and 985 ± 122 dogs (BS) showed slightly lower mutagenic effects. In addition, NaCl and other salts showed high mutants of 1671 to 2084 at 25% high salt concentration, but bamboo salt showed 1254 ± 17 low mutants. Considering that the high K content in bamboo salt and this K component has anti-cancer effects, the low mutagenicity of bamboo salt may be derived from the high K content unlike other salts.

Experimental Example 2 SOS Chromotest

In this experiment, we used PQ37, a variant of E. coli, to examine the effects of mutations in SOS chromotest. In PQ37, the normal lacZ gene was removed and the sfiA gene was inserted to repressor protein. It is locked by. When genotoxic substances come in, they are splitted and lacz gene is expressed, resulting in β-galactosidase activity. ONPG and PNPP are used as colorants. After a dose response to NaCl, MNNG was treated with 20 µg per well and the concentration of NaCl was treated with 0.1 ~ 600 µg. In other words, E.coli PQ37 / plasmid 101 (PQ37) derived from E.coli GC4436 and containing the sfiA gene in the lac Z gene was used. , rfa mutation and the composition of PHO ^c gene and induction of sfiA :: lacZ fusion were used in the experiment. The mutagenic effect test was a method modified from Quillard et al. 50 µl of frozen-stored PQ37 bacteria solution was inoculated into 5 mL culture, shaken overnight at 37 ° C., and then inoculated in 5 mL L culture medium and incubated at 37 ° C. for 2 hours (A660 0.3 to 0.4). The obtained bacterial solution was diluted 1/10 in L culture medium, and 100 µl of each well of each 96-well plate in which 10 µl of each sample and mutagen were previously dispensed, and shaken at 37 ° C for 90 minutes to induce SOS reaction. Later, 100 μl of ONPG was used to measure the activity of β-galactosidase (β-G), and 100 μl of PNPP was measured to measure the activity of alkaline phosphatase (AP). The color development time was 5 minutes, β-galactosidase was 100M of 1.5M Na ₂ CO ₃ , SP was 50L of 1M HCl. HCl was neutralized by addition and the absorbance was measured at 420 nm with a spectrophotometer. The measured OD 420nm measured enzyme value (Eu) by Miller's formula.

Eu = (1000 × A ₄₂₀ ) ÷ t (min)

As a result, it was confirmed that β-galactosidase activity was increased up to 0.1 ~ 100㎍ treatment, showing a mutated effect at 10 ~ 100㎍ treatment per well, and the activity of alkaline phosphatase was similar to that of the control group. There was no toxicity at the concentration. The results of treatment of 20 ~ 100 ㎍ per well by salt distillation using this experimental system are shown in Tables 4 and 5. In Table 4, Han-geum showed the mutagenic effect on MNNG when treated with 20 ㎍. Hanjugeum, sun-dried salt, and fresh salt had weak mutagenic effect at 100㎍ treatment, but there was no baked salt and salt, whereas bamboo salt showed antimutagenic effect. As a result of examining the effect of mutation on AFB1 in Table 5, the effect of weak mutation on NaCl, Hanju Gold, Sea Salt, Fresh Salt, and Baked Salt was shown at 100 μg per well. Antigens and bamboo salts showed antimutagenic effects at 20 and 100 ㎍, respectively. Therefore, natural salt, raw salt and processed salt, salt and bamboo salt had lower mutagenic effect than mechanical salt, and bamboo salt showed antimutagenic effect in SOS experimental system. Eventually, the effect was somewhat different according to the mutant experimental system, but bamboo salt was the safest, and mutagenic activity was affected by salt concentration.

Mutation Effect of Various Salts According to Concentration Difference in Inducing SOS Function by MNNG Treatment of E.coli PQ37 Sample concentration β-galactosidase (β) Alkaline phosphatase (ρ) (β) / (ρ) SOS Inducer OD ₄₂₀ Unit OD ₄₂₀ Unit Natural Return Mutations 0.60 ± 0.04 29.9 0.29 ± 0.04 14.5 2.1 1.0 1.30 ± 0.07 66.5 0.28 ± 0.05 13.9 4.8 2.3 20 μg / plate NaCl for reagents 1.56 ± 0.04 78.0 0.34 ± 0.01 17.0 4.6 2.2 One address 1.48 ± 0.07 74.2 0.29 ± 0.04 14.6 5.1 2.4 Sun salt 1.41 ± 0.16 70.7 0.32 ± 0.08 16.2 3.5 1.7 Fresh salt 1.42 ± 0.14 71.0 0.43 ± 0.09 21.6 3.3 1.6 Baked Salt 1.49 ± 0.13 72.1 0.38 ± 0.00 18.9 3.8 1.5 Salary 1.36 ± 0.10 68.2 0.39 ± 0.13 23.0 3.0 1.4 Bamboo salt 1.32 ± 0.16 66.2 0.43 ± 0.09 22.6 2.9 1.4 100 μg / plate NaCl for reagents 1.65 ± 0.06 82.5 0.30 ± 0.05 16.8 4.9 2.3 One address 1.80 ± 0.09 90.1 0.36 ± 0.07 17.8 5.1 2.4 Sun salt 1.60 ± 0.07 80.2 0.32 ± 0.03 16.0 5.0 2.4 Fresh salt 1.62 ± 0.05 81.0 0.30 ± 0.06 15.2 5.3 2.5 Baked Salt 1.59 ± 0.01 79.3 0.33 ± 0.06 16.6 4.8 2.3 Salary 1.56 ± 0.20 77.9 0.36 ± 0.00 16.8 4.6 2.2 Bamboo salt 1.51 ± 0.20 75.4 0.30 ± 0.06 22.3 3.4 1.6

Mutational Effects of Different Salts with Different Concentrations in Inducing SOS Function by Aflatoxin (AFB1, 10ng / assay) in E. coli PQ37 Sample concentration β-galactosidase (β) Alkaline phosphatase (ρ) (β) / (ρ) SOS Inducer OD ₄₂₀ Unit OD ₄₂₀ Unit Natural Return Mutations 0.71 ± 0.05 35.5 0.84 ± 0.03 42.2 0.8 1.0 1.88 ± 0.03 94.0 0.85 ± 0.01 42.6 2.2 2.8 20 μg / plate NaCl for reagents 1.63 ± 0.05 81.6 0.86 ± 0.07 43.1 1.9 2.4 One address 2.07 ± 0.10 103.4 0.97 ± 0.13 48.5 2.1 2.6 Sun salt 1.96 ± 0.29 97.8 0.96 ± 0.00 47.9 2.0 2.5 Fresh salt 1.86 ± 0.07 93.2 0.85 ± 0.09 42.5 2.1 2.5 Baked Salt 1.69 ± 0.03 84.4 0.83 ± 0.02 41.3 2.0 2.6 Salary 1.66 ± 0.12 83.1 0.86 ± 0.17 42.8 1.9 2.5 Bamboo salt 1.78 ± 0.01 89.0 0.88 ± 0.05 43.9 2.0 2.5 100 μg / plate NaCl for reagents 2.14 ± 0.26 106.8 0.90 ± 0.10 45.0 2.4 3.0 One address 2.13 ± 0.08 106.5 0.85 ± 0.05 42.7 2.5 3.1 Sun salt 2.02 ± 0.12 101.2 0.81 ± 0.00 40.7 2.5 3.1 Fresh salt 2.18 ± 0.10 109.0 0.86 ± 0.03 42.8 2.5 3.1 Baked Salt 1.89 ± 0.00 94.7 0.87 ± 0.03 43.3 2.1 3.1 Salary 1.71 ± 0.09 85.4 0.85 ± 0.02 42.7 2.0 2.6 Bamboo salt 1.83 ± 0.11 91.5 0.91 ± 0.16 45.4 2.0 2.5

As described above through the above examples and experimental examples, bamboo salts rich in K have no peroxidation promoting ability and low antimutagenic effects due to carcinogens compared to salts with high Na content. It is an industrially useful invention.

Claims (1)

Anticancer bamboo salt that does not have peroxidation promoting ability and inhibits mutations caused by carcinogens.