KR19990069399A - Onion Fermented Wine Manufacturing Method - Google Patents

Onion Fermented Wine Manufacturing Method Download PDF

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KR19990069399A
KR19990069399A KR1019980003620A KR19980003620A KR19990069399A KR 19990069399 A KR19990069399 A KR 19990069399A KR 1019980003620 A KR1019980003620 A KR 1019980003620A KR 19980003620 A KR19980003620 A KR 19980003620A KR 19990069399 A KR19990069399 A KR 19990069399A
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onion
juice
main
sugar
added
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KR100256772B1 (en
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김희대
서전규
정은호
이찬중
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황필생
김강권
대한민국(관리부서:농촌진흥청)
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • C12G3/026Preparation of other alcoholic beverages by fermentation with health-improving ingredients, e.g. flavonoids, flavones, polyphenols or polysaccharides, added before or during the fermentation stage; with flavouring ingredients added before or during the fermentation stage

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Abstract

본 발명은 양파를 이용한 발효주의 제조방법에 관한 발명이다. 양파는 가공품 개발이 미진할 뿐만 아니라 양파의 주요성분인 유기유황 화합물에 의해서 발효가 어려운 단점을 개선하기 위하여 본 발명은 양조용 효모의 배양과 주모액의 조제 및 본 발효를 위한 주 발효액의 최적화를 통해 문제점을 해결하였다.The present invention relates to a method for producing fermented wine using onions. In order to improve the disadvantages that the onion is not well developed and fermentation is difficult due to the organic sulfur compound which is the main ingredient of the onion, the present invention is to cultivate brewing yeast and preparation of the main broth and optimization of the main fermentation broth This solved the problem.

양조용효모를 고체배지에 배양하고 이를 사면배지에 25℃에서 2~3일간 배양하고 액체배지에 고체배지에서 배양된 균주를 접종하여 25℃에서 2~3일간 다시 배양한다. 주모액 조제는 양파를 착즙하여 착즙액에 10%정도 설탕을 첨가하여 살균후 액체배양된 균주를 약 10% 접종후 25℃에서 2~3일간 배양한다. 주 발효액 조제는 양파를 박피 세척하여 착즙후 착즙액에 당함량이 25%되게 설탕을 첨가하여 살균후 주모액을 5% 접종하여 25℃에서 발효시켜 양파주를 제조한다.The brewing yeast is incubated in a solid medium and incubated for 2 to 3 days at 25 ° C. in a slope medium, and then inoculated with the strain cultured in a solid medium to a liquid medium and re-incubated at 25 ° C. for 2 to 3 days. The main mother juice is prepared by sterilizing onions, adding about 10% sugar to the juice, sterilizing the liquid cultured strain, and incubating at 25 ° C. for 2 to 3 days after inoculation of about 10%. Preparation of the main fermentation broth after peeling and washing the onion peeled juice added sugar to the juice content of 25%, after sterilization 5% inoculated main liquid and fermented at 25 ℃ to prepare the onion wine.

Description

양파 발효주의 제조방법Manufacturing method of onion fermented wine

양파는 재배역사가 오래된 조미용 식물로 우리나라에서도 매년 많은 면적이 재배되고 있고 마늘, 파, 고추등과 더불어 식생활에 없어서는 안될 주요 채소중의 하나이다. 양파는 내한성 식물체로 여러 가지 기후 조건에서 생육할 수 있어 거의 전세계에서 재배하고 있으며 오래전부터 향신료와 함께 식용한 기록이 있으나 자르면 눈물이 날 정도로 톡 쏘는 듯한 냄새와 매운맛 때문에 널리 보급되지는 않았다. 최근에는 양파 특유의 냄새 때문에 향신용 식물로 보급과 더불어 양파의 주요 성분인 유기유황화합물들의 항균작용, 혈압강하, 혈액순환 촉진, 콜레스테롤 저하, 당뇨병 예방 및 치료, 항암효과등이 알려지고 있고 생리활성 효과 및 약리성에 대한 연구가 활발해지고 있어 관심이 더욱 고조되고 있다. 이러한 양파의 소비증대를 위해서는 새로운 식품개발 방안이 강구되어야 할 것이다. 그러나 가공품 연구개발이 미진하고 최근 양파김치, 스낵, 음료등이 개발되고 있으나 판매부진등 어려움이 있고 양파장아찌, 식초, 건조양파, 양파분말등이 생산되고 있으나 소비자의 만족을 시키지 못하고 있어 새로운 가공품 개발이 절실한 실정이다.Onion is a seasoning plant with a long history of cultivation. In Korea, a large area is cultivated every year, and it is one of the indispensable vegetables along with garlic, green onion, and pepper. Onion is a cold-tolerant plant and can be grown under various climatic conditions. It is grown almost all over the world, and it has been eaten with spices for a long time, but it has not been widely used because of its pungent smell and pungent taste. Recently, due to the peculiar smell of onion, it is widely distributed as a spice plant, and the antibacterial activity of organic sulfur compounds, which are the main components of onion, lowering blood pressure, promoting blood circulation, lowering cholesterol, preventing and treating diabetes, and anticancer effects are known. The research on the effects and pharmacology is getting active, and the interest is getting higher. In order to increase the consumption of onions, new food development plans should be devised. However, research and development of processed products is insufficient, and recently, onion kimchi, snacks, and drinks are being developed, but there are difficulties such as sluggish sales, and onion pickles, vinegar, dried onions, onion powder, etc. are being produced. This is an urgent situation.

본 발명은 종래의 양파의 용도가 조미료 또는 가공식품의 첨가물에 사용되는 것에서 다양한 용도를 확대하여 양파의 독특한 향과 색깔을 함유한 양파주의 제조공정을 확립하고자 하였다. 양파는 주요성분인 유기유황 화합물에 의해서 발효가 어려운 단점이 있어 이를 개선하기 위하여 시험을 거듭한 결과 양파 발효주 제조시 양파즙액의 당함량과 발효온도를 최적화시켜 발효가 양호하고 알콜농도가 높은 양파 발효주를 제조할 수 있었다. 본 발명은 고체배지에 양조용효모를 사면배지에 배양하고 액체배지에 고체배지에서 배양된 균주를 접종하여 균주를 배양하고, 양파 착즙액에 설탕을 첨가하여 적정 발효온도에서 발효시켜 다양한 알콜농도의 향미성 있는 양파주의 제조방법을 제공함에 있다.The present invention has been intended to establish a process for producing onion wine containing the unique flavor and color of onions by expanding the various uses from the use of conventional onions used in seasoning or processed food additives. Onion has the disadvantage that fermentation is difficult due to the organic sulfur compound, which is a major ingredient, and the results of repeated tests to improve it are onion fermented liquor with good fermentation and high alcohol concentration by optimizing the sugar content and fermentation temperature of onion juice liquor Could be prepared. The present invention is to cultivate the yeast for brewing yeast in solid medium and inoculate the strain cultured in solid medium in liquid medium, to cultivate the strain, and to add sugar to the onion juice solution to ferment at an appropriate fermentation temperature of various alcohol concentrations The present invention provides a method for producing a flavorful onion wine.

도 1은 양파주 제조 공정도1 is a process for producing onion wine

본 발명은 균주배지조제 및 균주배양, 주모액조제, 주발효액조제 및 발효 양파주 제조의 3단계 공정으로 이루어져 있다. 다음의 실시예는 본 발명의 예시에 불과하며 본원 발명의 기술적 범위를 한정하지는 않는다.The present invention consists of a three-step process of the preparation of strain medium and strain culture, preparation of main mother liquor, main fermentation broth and fermented onion wine. The following examples are merely illustrative of the invention and do not limit the technical scope of the invention.

실시예 1Example 1

균주 배양을 위한 배지의 조성은 표 1과 같이 하여 양조용효모(KCTC1218)을 사면배지에 25℃에서 2-3일간 배양하고 표 1의 액체배지에 고체배지에서 배양된 균주를 접종하여 25℃에서 2-3일 배양한다.The composition of the culture medium for strain cultivation is as shown in Table 1 cultured brewing yeast (KCTC1218) in a slope medium for 2-3 days at 25 ℃ and inoculated strain cultured in a solid medium in the liquid medium of Table 1 at 25 ℃ Incubate 2-3 days.

[표 1] 배지 조성표(YM배지)[Table 1] Medium composition table (YM medium)

실시예 2Example 2

양파를 착즙하여 착즙액의 10중량%의 설탕을 첨가하여 살균후 실시예 1의 배양액을 10중량% 접종하여 25℃에서 2-3일 배양한다. 이때 처리전 양파즙액의 특성은 pH5.7이었고, 당도가 7.4정도로 비교적 높은 편이었다. (표 2 참조)Onion is juiced and 10% by weight of sugar is added to the juice after sterilization and 10% by weight of the culture solution of Example 1 is incubated at 25 ° C. for 2-3 days. At this time, the characteristics of onion juice before treatment was pH5.7, and the sugar content was relatively high, about 7.4. (See Table 2)

[표 2] 양파즙액 특성[Table 2] Onion juice

주1) 구연산으로 환산한 수치임Note 1) Values are converted to citric acid.

실시예 3Example 3

양파를 박피 세척하여 착즙후 착즙액에 당함량이 25%가 되게 설탕을 첨가하여 살균후 실시예 2의 주모액을 설탕이 첨가된 착즙액의 5중량% 접종하여 25℃에서 발효하여 양파주를 제조한다.After peeling and washing the skin peeled with juice, sugar was added to the juice solution to 25%, and after sterilization, the mother liquor of Example 2 was inoculated with 5% by weight of the juice solution to which sugar was added and fermented at 25 ° C. Manufacture.

공시재료는 중만생종 양파인 창녕대고를 이용하여 당첨가량을 무조절, 15%조절, 25%조절, 35%조절로 하여 시험을 수행하였다.The test materials were tested using the Changnyeong Daego, a medium-grown onion, with no addition, 15%, 25%, and 35%.

시기별 pH는 발효 2일째부터 떨어져서 처리별 발효일수별 발효기간내 큰 변화가 없었다 (표 3 참조)The pH at each time dropped from the 2nd day of fermentation, so there was no significant change in the fermentation period by fermentation days by treatment (see Table 3).

[표 3] 시기별 pH변화[Table 3] pH change by time

주1) 처리별 3회 반복 조사한 수치임Note 1) 3 times of treatment

주2) pH Meter(Fisher Scientific accument pH Meter 25)Note 2) pH Meter (Fisher Scientific accument pH Meter 25)

시기별 당도는 발효가 되면서 차츰 떨어져서 발효 9일째부터 변화가 거의 일어나지 않았다.(표 4 참조)The sugar content of each season gradually dropped as it entered into effect, and hardly changed from the 9th day of fermentation (see Table 4).

[표 4] 시기별 당도변화[Table 4] Change in sugar content by period

주1) 처리별 3회 반복 조사Note 1) 3 repetition surveys per treatment

주2) Hand Refractometer(N.O.W. Tokyo)Note 2) Hand Refractometer (N.O.W.Tokyo)

시기별 총산 변화는 처리전이나 발효가 진행되나 차이가 없었다.(표 5 참조)Changes in total acidity over time were not different between pretreatment and fermentation (see Table 5).

[표 5] 시기별 총산변화(%)[Table 5] Total Change in Season (%)

주1) 처리별 3회 반복 조사Note 1) 3 repetition surveys per treatment

주2) 0.1N-NaOH적정법으로 실시(Citric acid 환산 수치임)Note 2) 0.1 N-NaOH titration method (citric acid equivalent)

발효가 진행됨에 따라 알콜의 함량이 차츰 증가하다가 9일째부터 큰 변화가 없었다.(표 6 참조)As the fermentation progressed, the alcohol content gradually increased, but there was no significant change from the 9th day (see Table 6).

[표 6] 시기별 알콜변화[Table 6] Alcohol change by time

주1) 간이 주정계 측정Note 1) Simple alcohol gauge measurement

시기별 색도의 변화는 25% 및 35%처리에서 약간의 갈변현상이 나타났다.The change of chromaticity by time showed slight browning at 25% and 35% treatment.

[표 7] 시기별 색도변화[Table 7] Chromaticity change by time

주1) L : 명도/100, a : 적색방향/60, b : 황색방향/60Note 1) L: Brightness / 100, a: Red direction / 60, b: Yellow direction / 60

주2) 측정기기 : Minolta Chroma meter CT-210 Cell 10mmNote 2) Measuring device: Minolta Chroma meter CT-210 Cell 10mm

알콜의 수득율은 처리간에 비슷한 경향이었다.Yields of alcohol tended to be similar between treatments.

[표 8] 알콜 수득률TABLE 8 Alcohol Yield

케르세틴(Quercetin) 함량은 당첨가량을 25%로 했을 때 가장 높았다.Quercetin content was highest when the amount of winnings was 25%.

[표 9] 케르세틴 함량Table 9 Quercetin Content

1) HPLC 분석 (Dionex Dx-300)1) HPLC Analysis (Dionex Dx-300)

양파는 가공품 개발이 미진할 뿐만 아니라 양파의 주요성분인 유기유황 화합물에 의해서 발효가 어려운 단점이 있다. 이러한 점을 개선하기 위하여 본 발명은 양조용 효모의 배양과 주모액의 조제 및 본 발효를 위한 주발효액의 최적화를 확립하여 알콜농도가 양호하고 케르세틴 함량이 높은 양파 발효주의 제조방법으로써 양파의 용도 확대는 물론 비상품성의 양파로도 가공제품을 만들 수 있다.Onions are not well developed and have a disadvantage in that fermentation is difficult due to organic sulfur compounds, which are the main components of onions. In order to improve this point, the present invention establishes the cultivation of brewing yeast, preparation of main liquid and optimization of main fermentation liquid for the main fermentation, and expansion of the use of onion as a method of producing onion fermented wine with high alcohol concentration and high quercetin content. Non-commercial onions can also be used to make processed products.

Claims (4)

양조용 효모(KCTC1218)를 고체배지에 배양한 균주를 액체배지에 다시 배양하고, 설탕을 첨가한 양파 착즙액에 배양액을 접종하여 주모액을 만들고, 양파를 박피, 세척, 착즙하고 살균한 착즙액에 설탕을 첨하고 여기에 주모액을 접종하여 20~30℃에서 2~14일간 발효시키는 것을 특징으로 하는 양파 발효주의 제조방법.The strain cultured with brewing yeast (KCTC1218) in a solid medium was re-incubated in a liquid medium and inoculated with a culture solution in a sugar-added onion juice to make a main mother's solution, and peeled, washed, juiced and sterilized onion. The method of producing onion fermented liquor characterized in that the fermentation at 2-30 days at 20 ~ 30 ℃ by inoculating sugar and added to the main mother liquor. 제 1 항에 있어서,The method of claim 1, 효모의 고체배지는 효모추출액(Yeast Extract) 2~4g, 맥아추출액(Malt Extract) 2~4g, 펩톤(Peptone) 4~6g, 덱스트로스 8~12g, 아가(Agar) 16~24g, 증류수 1ℓ로하고, 액체배지는 효모추출액 2~4g, 맥아추출액 2~4g, 펩톤 4~6g, 덱스트로스 8~12g, 증류수 1ℓ로 하는 것을 특징으로 하는 양파 발효주 제조방법.The yeast solid medium is 2 ~ 4g yeast extract, 2 ~ 4g malt extract, 4 ~ 6g peptone, 8 ~ 12g dextrose, 16 ~ 24g agar, 1l distilled water And, the liquid medium of the fermented wine production method of onion fermented liquor, characterized in that the yeast extract 2 ~ 4g, malt extract 2 ~ 4g, peptone 4 ~ 6g, dextrose 8 ~ 12g, distilled water 1L. 제 1 항에 있어서,The method of claim 1, 설탕을 양파 착즙액의 5~15중량%를 첨가하고, 배양액을 설탕이 첨가된 양파 착즙액의 5~15중량%로 접종하여 주모액을 제조하는 것을 특징으로 하는 양파 발효주 제조방법.Sugar is added to 5 to 15% by weight of the onion juice solution, onion fermentation liquor production method characterized in that the culture solution is inoculated with 5 to 15% by weight of the sugar juice added onion. 제 1 항에 있어서,The method of claim 1, 양파의 착즙액에 설탕을 15~35% 첨가하고, 주모액을 설탕이 첨가된 착즙액의 3~7% 접종하는 것을 특징으로 하는 양파 발효주 제조방법.15 to 35% of sugar is added to the juice of the onion, and the main fermented liquor is inoculated with 3-7% of the juice added with sugar.
KR1019980003620A 1998-02-09 1998-02-09 Brewing process of onion wine KR100256772B1 (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100504094B1 (en) * 2003-03-28 2005-07-27 장석진 Liquid ferment for brewing, and fermented alcoholic beverages using that
KR100517062B1 (en) * 2000-12-29 2005-09-26 현대영농조합법인 Onion-vinegar and process for preperation thereof
KR100739226B1 (en) * 2005-06-01 2007-07-13 창녕군 Method for preparing wine using onion and onion wine obtained by the method
CN101085970B (en) * 2007-07-02 2010-10-06 北京市科威华食品工程技术有限公司 Micro-ferment onion grape wine process technology
KR101143598B1 (en) * 2010-04-15 2012-05-09 천보레미콘 주식회사 onion liquor

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100841642B1 (en) * 2007-03-19 2008-06-26 박정이 Producing process an alcoholic beverage using an onion

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100517062B1 (en) * 2000-12-29 2005-09-26 현대영농조합법인 Onion-vinegar and process for preperation thereof
KR100504094B1 (en) * 2003-03-28 2005-07-27 장석진 Liquid ferment for brewing, and fermented alcoholic beverages using that
KR100739226B1 (en) * 2005-06-01 2007-07-13 창녕군 Method for preparing wine using onion and onion wine obtained by the method
CN101085970B (en) * 2007-07-02 2010-10-06 北京市科威华食品工程技术有限公司 Micro-ferment onion grape wine process technology
KR101143598B1 (en) * 2010-04-15 2012-05-09 천보레미콘 주식회사 onion liquor

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