KR20100007663A - Brewing with natural broth ferment containing red koji and making confectionary, bread, steamed rice-cake with red koji ferment in it - Google Patents

Brewing with natural broth ferment containing red koji and making confectionary, bread, steamed rice-cake with red koji ferment in it Download PDF

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KR20100007663A
KR20100007663A KR1020080068847A KR20080068847A KR20100007663A KR 20100007663 A KR20100007663 A KR 20100007663A KR 1020080068847 A KR1020080068847 A KR 1020080068847A KR 20080068847 A KR20080068847 A KR 20080068847A KR 20100007663 A KR20100007663 A KR 20100007663A
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fermented
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red yeast
red koji
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김영은
정상미
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/04Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
    • C12G3/05Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs with health-improving ingredients, e.g. flavonoids, flavones, polyphenols or polysaccharides
    • AHUMAN NECESSITIES
    • A21BAKING; EDIBLE DOUGHS
    • A21DTREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D13/00Finished or partly finished bakery products
    • A21D13/06Products with modified nutritive value, e.g. with modified starch content
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10Cereal-derived products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • C12G3/026Preparation of other alcoholic beverages by fermentation with health-improving ingredients, e.g. flavonoids, flavones, polyphenols or polysaccharides, added before or during the fermentation stage; with flavouring ingredients added before or during the fermentation stage

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Abstract

PURPOSE: A method for producing red yeast rice using liquid medium is provided to prevent citrinin generation which is toxic material and enhance monacolin-K content. CONSTITUTION: A method for producing fermented liquor of red yeast rice comprises: a step of mixing honey bee liquid, sugar liquid, and various fruits and fermenting Monascus; a step of making bread with fermented liquid seed; a step of making cookies with the fermented liquid as an additive; and a step of making wine rice cake.

Description

천연 영양원을 이용한 홍국 발효주 제조 방법과 이를 발효액종으로 하는 제과, 제빵, 술떡의 제조{Brewing with natural broth ferment containing red koji and making confectionary, bread, steamed rice-cake with red koji ferment in it}Method of manufacturing fermented liquor of red yeast rice using natural nutrients and manufacturing of confectionery, bakery, and liquor using the same as fermented broths {Brewing with natural broth ferment containing red koji and making confectionary, bread, steamed rice-cake with red koji ferment in it}

본 발명은 Monascus 액체 배양을 위한 탄소원으로 glucose, fructose를 많이 함유하고 있는 벌꿀용액 , 당용액, 각종 과일 즙 등을 천연 영양원으로 하여 액체 발효함으로써 고체발효 시 생산될 수 있는 citrinin의 생성을 방지하였다. 또한 천연 영양원으로 Monascus를 발효시켜 발효가 진행될수록 monacolin-K 함량이 증가되고 홍국 특유의 향은 퇴색하여 단향과 착향성이 증가되며 적색소는 퇴색하여 황금빛을 나타내 기능적으로나 기호도가 증가된 홍국 발효주를 제조하였다. 그리고 이 발효주를 발효액종으로 하여 제과, 제빵 등을 제조함으로써 기존의 홍국 분말 첨가로 인한 제빵 품질 및 기호성 저하의 문제점을 해결하고 혈중 콜레스테롤의 함량저하 효과를 가지고 있으며 음식물과 함께 투여했을 때 혈중농도가 공복 시 보다 높아지는 monacolin-K를 기능성 식품으로서 따로 섭취해야 하는 불편함 없이 식사나 간식으로 자연스럽게 섭취하고자 한다.The present invention prevents the production of citrinin which can be produced during solid fermentation by liquid fermentation of honey solution, sugar solution, various fruit juices, etc., which contain glucose and fructose as a carbon source for Monascus liquid culture. In addition, fermentation of Monascus as a natural nutrient source increases monacolin-K content and the flavor of red yeast rice is faded to increase its sweetness and flavor. The red pigment fades to show golden color and functionally increased taste. Prepared. The fermented liquor is used as a fermentation broth to produce confectionery and bakery, thereby solving the problems of deterioration of baking quality and palatability due to the addition of conventional red yeast powder, and having a blood cholesterol content lowering effect. Monacolin-K, which is higher on an empty stomach, is intended to be taken naturally as a meal or snack without the inconvenience of having to consume it separately as a functional food.

홍국(red yeast rice)은 찐 백미에 Monascus 속 곰팡이를 증식시켜 제조한 홍색의 코지(koji)로 다른 국(麴)에 비해 알코올 생산 능력이 강하고 단향과 착향성이 우수하여 옛날부터 주로 술, 홍유부, 고기 및 야채의 절임 등의 제조에 이용되어 왔을 뿐만 아니라 적색의 천연색소로 널리 사용해 왔으며, 한방생약으로도 이용되어 왔다 (1)Red yeast rice is a red colored koji made by growing fungi of Monascus on steamed white rice. It has been used not only for the production of pickles, meat and vegetables, but also as a red natural pigment and as a herbal medicine (1)

또한 홍국은 약리효과도 가지고 있는데 순환기계와 관련된 성분으로 강력한 cholesterol 생합성 저해물질인 lovastatin(monacolin-K)을 생산하는 것을 발견하였으며(2) Monacolin-K는 미국식품의약품 (United States Food Drug Administration)에 의해 승인된 (1987) 최초의 스타틴으로서 항 콜레스테롤의 약물로 이용되고 있다. (3)Red ginseng also has a pharmacological effect, which is found to produce lovastatin (monacolin-K), a potent cholesterol biosynthesis inhibitor, as a component of the circulatory system. (2) Monacolin-K has been added to the United States Food Drug Administration. First approved statins (1987), it is used as an anti-cholesterol drug. (3)

Monacolin-K는 콜레스테롤 생합성 속도를 제한하여 혈관 속의 콜레스테롤 수치를 가장 효과적으로 감소시킨다고 알려져 있어 콜레스테롤 증 환자의 치료에 선호되고 있다.(4) 특히, 음식물과 함께 투여했을 때 혈중농도가 공복 시 보다 1.5배 높아지므로 음식과 함께 복용하도록 추천하고 있다.(5)Monacolin-K is known to be the most effective at reducing cholesterol levels in blood vessels by limiting the rate of cholesterol biosynthesis, and is therefore preferred for the treatment of hypercholesterolemia. (4) Blood levels, especially when taken with food, are 1.5 times higher than on fasting. It is recommended to take with food as it is high. (5)

최근에는 많은 연구자들이 HMG-CoA reductase 억제물질인 홍국유래 statins가 지방세포분화를 조절한다고 보고하였다.(6)Recently, many researchers have reported that red yeast-derived statins, HMG-CoA reductase inhibitors, regulate adipocyte differentiation.

오늘날과 같은 발효빵의 역사는 기원전 3000년경에 이집트에서 시작되어 그리스를 거처 로마로 전파되어 유럽 각지로 퍼져 나갔다(7). 그 뒤 1857년 프랑스의 파스퇴르(L. Pasteur)가 효모의 작용을 발견함(8)에 따라 순수 배양이 가능한 이스트가 상품으로 만들어 지면서 제빵법의 체계화가 이루어 졌다.Today's history of fermented bread began in Egypt around 3000 B.C.E., spread through Greece to Rome, and spread throughout Europe (7). Later, in 1857, French Pasteur L. Pasteur discovered the action of yeast (8), which led to the systematization of the bakery method, making pure cultivable yeast into products.

한편 우리나라에 빵이 전해진 것은 구한말 외국인 선교사들에 의해 시작되었으며 1970년대 이후 분식장려 운동을 계기로 본격화 되었다.On the other hand, bread was introduced to Korea by foreign missionaries from the late Hankuk-mal, and it began in earnest after the 1970s with the encouragement of snacking.

이후 생활수준의 향상과 함께 건강식품에 대한 관심과 욕구의 증가로 빵의 제조에 있어 기능성 물질의 첨가에 대한 연구가 활발히 이루어지고 있다.Since then, with the improvement of living standards and the increase in interest and desire for health foods, research on the addition of functional substances in the manufacture of bread has been actively conducted.

본 발명에서는 혈중 콜레스테롤의 함량저하 효과를 가지고 있으며 음식물과 함께 투여했을 때 혈중농도가 공복 시 보다 1.5배 높아지(5)는 Monascus를 액침배양하여 추출물속의 monacolin K를 최근 식생활 변화에 따라 증가하고 있는 건강 빵에 적용하였다.In the present invention has a blood cholesterol-lowering effect and when administered with food, blood concentration is 1.5 times higher than when fasting (5) immersed in Monascus to increase the monacolin K in the extract according to the recent dietary changes Applied to bread.

[문헌1]. Tarui S. Development and utility of red mold rice. Shokuhin to kaihatsu 28:47-50 (1993).[Document 1]. Tarui S. Development and utility of red mold rice. Shokuhin to kaihatsu 28: 47-50 (1993).

[문헌2]. Endo A. Monacolin K, new hypocholesterolemic agent produced by a monascus species. J. Antibiotics 32: 852-854 (1979)[Document 2]. Endo A. Monacolin K, new hypocholesterolemic agent produced by a monascus species. J. Antibiotics 32: 852-854 (1979)

[문헌3]. Kirby TJ. Cataracts produced by triparanol (MER-29). Trans Am Ophthalmol Soc 65:494-543 (1967)[Reference 3]. Kirby TJ. Cataracts produced by triparanol (MER-29). Trans Am Ophthalmol Soc 65: 494-543 (1967)

[문헌4]. Slater EE, JS MacDonold. Mechanism of action and biological profile of HMG-CoA reductase inhibitors. a new therapeutic alternative Drugs 36: 72-82(1988)[Document 4]. Slater EE, JS MacDonold. Mechanism of action and biological profile of HMG-CoA reductase inhibitors. a new therapeutic alternative drugs 36: 72-82 (1988)

[문헌5]. Wong HC, Bau YS. Pigmentation and antibacterial activity of fast neutron-and X-ray-induced strains of Monascus purpures went. Plant Physiol 60:578-581(1977)[Document 5]. Wong HC, Bau YS. Pigmentation and antibacterial activity of fast neutron-and X-ray-induced strains of Monascus purpures went. Plant Physiol 60: 578-581 (1977)

[문헌6]. Slater EE and JS MacDonold. 1988. Mechanism of action and biological profile of HMG-CoA reductase inhibitors. a new therapeutic alternative Drugs 36: 72-82[Document 6]. Slater EE and JS MacDonold. 1988. Mechanism of action and biological profile of HMG-CoA reductase inhibitors. a new therapeutic alternative Drugs 36: 72-82

[문헌7]. Karise, T. The process of Pan making. Numade Shouten Co., Tokyo, p.27-28 (1984)[7]. Karise, T. The process of Pan making. Numade Shouten Co., Tokyo, p.27-28 (1984)

[문헌8].The monthly Bakery. Theoretical confectionery bread . B & C WORLD CO.,LTD pp 9-10 (2000)8 The monthly Bakery. Theoretical confectionery bread. B & C WORLD CO., LTD pp 9-10 (2000)

본 발명은 천연 영양원을 이용한 액체발효법으로 홍국발효주를 제조하여 일반적인 국의 역할뿐만 아니라 고체발효법에 의한 홍국 발효 시 생산될 수 있는 mycotoxin의 일종인 citrinin의 생성을 방지하고자 하였다.The present invention was intended to prevent the production of citrinin, a kind of mycotoxin that can be produced during fermentation of red yeast rice by the solid fermentation method as well as the production of red yeast fermented liquor by liquid fermentation method using natural nutrients.

또한 본 발명에서는 이 발효주를 발효액종으로 하여 홍국발효 액종을 첨가한 빵을 만들어 홍국이 발효가 진행될수록 monacolin-K함량과 단향과 착향성은 증가시키고 적색소는 퇴색시킨 액체발효 홍국을 첨가함으로써 홍국 특유의 향과 색에 의한 제빵 품질과 기호성 저하의 문제점을 해결하고자 하였으며 액종 발효 시 생산되는 Monascus 속의 monacolin-K의 양은 최대한 증가시켜 합리적인 기능성 제과, 제빵을 생산하고자 하였다.In addition, in the present invention, the fermented liquor is used as a fermentation broth to make bread containing fermented soybean paste, and as the fermentation proceeds, the monacolin-K content and the flavor and flavor are increased, and the red pigment is faded. The purpose of this study was to solve the problems of deterioration of baking quality and palatability due to the unique flavor and color, and to produce reasonable functional confectionery and bakery by increasing the amount of monacolin-K in Monascus, which is produced during the liquid fermentation.

본 발명은 Monascus속의 직접적인 영양원이 될 수 있는 glucose와 fructose 를 많이 함유하고 있는 벌꿀용액 , 당용액, 각종 과일 즙 등을 천연 영양원으로 하여 액체발효함으로써 고체발효법에 의한 홍국 발효 시 생산될 수 있는 mycotoxin의 일종인 citrinin의 생성을 방지하고자 하였다.The present invention is a mycotoxin that can be produced during fermentation of red yeast rice by solid fermentation by liquid fermentation with honey, sugar solution, and various fruit juices, which contain glucose and fructose, which can be direct nutrient sources of Monascus. The purpose of this study was to prevent the production of citrinin.

그리고 홍국발효주를 발효액종으로 첨가하여 제과, 제빵을 만들어 일반적인 국의 역할뿐만 아니라 홍국이 발효가 진행될수록 monacolin-K함량과 단향과 착향성은 증가되고 적색소는 퇴색됨으로 액체발효 홍국을 첨가함으로써 홍국 특유의 향과 색에 의한 제과, 제빵 품질과 기호성 저하의 문제점을 해결하고 발효액종 발효 시 생산되는 Monascus 속의 monacolin-K의 양은 최대한 증가시켜 합리적인 기능성 제빵을 생산 하였다.The fermented broth is added to fermented broth to make confectionery and bakery, and as the fermentation progresses, the monacolin-K content, sweetness and flavor increase, and red pigment fades. It solved the problems of confectionery, baking quality and palatability due to its unique flavor and color, and produced reasonable functional bakery by increasing the amount of monacolin-K in Monascus, which is produced during fermentation broth.

본 발명은 천연 영양원인 벌꿀용액 , 당용액, 각종 과일 즙 등으로 Monascus를 액체 발효함으로써 고체발효 시 생산될 수 있는 citrinin의 생성을 방지하였으며 발효가 진행될수록 monacolin-K 함량이 증가되었고 홍국 특유의 향은 퇴색하여 단향과 착향성이 증가되었으며 적색소는 퇴색하여 황금빛을 나타내 기능적으로도 기호도면에서도 증가된 홍국 발효주를 제조하였다. 그리고 이 발효주를 발효액종으로 하여 제빵과 제과 등을 제조함으로써 기존의 홍국 분말 첨가로 인한 제과, 제빵 품질 및 기호성 저하의 문제점을 해결하고 혈중 콜레스테롤의 함량저하 효과를 가지고 있으며 음식물과 함께 투여했을때 혈중농도가 공복 시 보다 1.5배 높아지는 monacolin-K를 기능성 식품으로서 따로 섭취해야 하는 불편함 없이 식사나 간식으로 자연스럽게 섭취하여 혈중 콜레스테롤 저하효과가 극대화 시켰다. 본 발명에서 Monascus 액체 발효를 위한 천영 영양원으로 하여 제조한 홍국주의 citrinin 비생성과 발효에 따른 monacolin-K의 증가와 이를 발효액종으로 한 제빵 및 제과 적성은 다음의 실시예에서 구체적으로 설명하고자 한다.The present invention prevents the production of citrinin that can be produced during solid fermentation by liquid fermentation of Monascus with natural nutritional sources such as honey solution, sugar solution, and various fruit juices. The red color faded to increase the flavor and flavor, and the red pigment faded to show the golden color to produce fermented red yeast rice functionally and also in the drawing. The fermented liquor is used as a fermentation broth to manufacture bakery and confectionery, which solves the problems of confectionery, bakery quality and palatability due to the addition of conventional red yeast powder, and has a blood cholesterol-lowering effect. Monacolin-K, which is 1.5 times higher than fasting concentration, is consumed naturally as a food or snack without the inconvenience of having to consume it separately as a functional food, maximizing blood cholesterol lowering effect. In the present invention, the increase in monacolin-K due to the non-producing citrinin and fermentation of red yeast wine prepared as a nutrient for nutrients for Monascus liquid fermentation, and the baking and confectionery aptitudes using the fermented liquid species will be described in detail in the following examples.

<실시예 1> pH를 Monascus 생육에 적절하게 조절한 천연 영양원인 벌꿀용액에 홍국분말 5%를 혼합한 후 25℃에서 교반하면서 7일간 발효한 홍국발효주의 citrinin 함량 분석결과는 표 1에서와 같이 전혀 검출되지 않았다.<Example 1> The citrinin content analysis results of fermented red yeast rice wine fermented for 7 days while stirring at 25 ℃ mixed 5% red yeast powder in a honey solution, a natural nutrient source adjusted to the pH of Monascus growth appropriately as shown in Table 1 It was not detected at all.

[표 1]TABLE 1

Figure 112008503670610-PAT00001
Figure 112008503670610-PAT00001

<실시예 2> 발효기간에 따른 홍국발효주의 monacolin-K 함량을 분석한 결과는 그래프 1과 같이 발효기간이 증가함에 따라 발효액종의 monacolin-K 함량이 증가하였다.<Example 2> As a result of analyzing the monacolin-K content of red yeast fermented wine according to the fermentation period, the monacolin-K content of the fermentation broth increased as the fermentation period increased as shown in Graph 1.

Figure 112008503670610-PAT00002
Figure 112008503670610-PAT00002

<실시예 3> 벌꿀용액을 영양원으로 하여 제조한 홍국주의를 발효액종으로 한 제빵 및 제과 적성에 대한 관능검사를 실시하여 표 2에 나타내었다.<Example 3> The sensory test for baking and confectionery aptitude using red yeast wine prepared with honey solution as a nutrient source is shown in Table 2.

[표 2]TABLE 2

Figure 112008503670610-PAT00003
Figure 112008503670610-PAT00003

도 1은 본 발명인 천연 영양원으로 홍국 발효주를 제조하는 방법의 일예를 나타낸 공정 도이다1 is a process chart showing an example of a method for producing red yeast fermented liquor by the present inventors natural nutrient source

도 2는 본 발명인 천연 영양원으로 발효한 홍국주를 발효액종으로 하여 빵을 제조하는 방법의 일예를 나타낸 공정도 이다Figure 2 is a process diagram showing an example of a method for producing bread using the fermented broth of red yeast rice fermented with a natural nutrition source of the present invention

도 3는 본 발명인 천연 영양원으로 발효한 홍국주를 첨가제로 하여 제과를 제조하는 방법의 일예를 나타낸 공정도 이다Figure 3 is a process diagram showing an example of a method of manufacturing a confectionery using red yeast rice fermented with the present natural nutrition source as an additive.

도 4는 본 발명인 천연 영양원으로 발효한 홍국주를 첨가제로 하여 술떨을 제조하는 방법의 일예를 나타낸 공정도 이다Figure 4 is a process diagram showing an example of a method for producing a wine liquor using red yeast rice fermented with a natural nutrient source of the present invention as an additive

Claims (4)

천연 영양원으로 벌꿀용액, 당용액, 각종 과즙을 각각 이용하는 단계와 천연 영양원으로 벌꿀용액, 당용액, 각종 과즙을 서로 하나 이상 혼합하여 이용하는 단계와 이를 영양원으로 하여 Monascus를 액체 발효하여 홍국 발효주를 제조방법Step of using honey solution, sugar solution and various juices as natural nutrients and mixing one or more of honey solution, sugar solution and various juices with natural nutrients and liquid fermentation of Monascus as a nutrient source. 제 1항의 천연 영양원으로 벌꿀용액, 당용액, 각종 과즙을 영양원으로 하여 Monascus를 액체발효한 홍국 발효주를 발효액종으로 하여 빵을 제조하는 방법The natural nutrient source of claim 1 is a method of producing bread using a fermented broth of red yeast fermented liquor, which is a liquid fermentation of Monascus, using honey solution, sugar solution, and various fruit juices as nutrients. 제 1항의 천연 영양원으로 벌꿀용액, 당용액, 각종 과즙을 영양원으로 하여 Monascus를 액체발효한 홍국 발효주를 첨가제로 하여 제과를 제조하는 방법Method of preparing confectionery using red yeast fermented liquor fermented with Monascus as an nutrient as a natural nutrient source of claim 1 as a nutrient as honey solution, sugar solution and various fruit juices. 제 1항의 천연 영양원으로 벌꿀용액, 당용액, 각종 과즙을 영양원으로 하여 Monascus를 액체발효한 홍국 발효주를 첨가제로 하여 술떡을 제조하는 방법The natural nutrition of claim 1 is a method for producing sake cake using red yeast fermented liquor fermented with Monascus liquid as a nutrient source of honey solution, sugar solution, and various juices
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102757874A (en) * 2012-06-28 2012-10-31 吉山红(福建)酒业有限公司 Red-rice yellow wine rich in lovastatin and brewing method of red-rice yellow wine
CN104522813A (en) * 2015-01-04 2015-04-22 哈尔滨伟平科技开发有限公司 Monascus healthy beverage preparing method
CN104920545A (en) * 2015-06-11 2015-09-23 河北工程大学 Red yeast rice biscuit and preparation method thereof
CN111602695A (en) * 2020-05-06 2020-09-01 江西中医药大学 Production formula and preparation method of biscuits colored and seasoned by traditional Chinese medicine components

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102757874A (en) * 2012-06-28 2012-10-31 吉山红(福建)酒业有限公司 Red-rice yellow wine rich in lovastatin and brewing method of red-rice yellow wine
CN104522813A (en) * 2015-01-04 2015-04-22 哈尔滨伟平科技开发有限公司 Monascus healthy beverage preparing method
CN104522813B (en) * 2015-01-04 2017-02-08 杨高林 Monascus healthy beverage preparing method
CN104920545A (en) * 2015-06-11 2015-09-23 河北工程大学 Red yeast rice biscuit and preparation method thereof
CN111602695A (en) * 2020-05-06 2020-09-01 江西中医药大学 Production formula and preparation method of biscuits colored and seasoned by traditional Chinese medicine components

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