KR19990064373A - Decoration method of product using DNA - Google Patents
Decoration method of product using DNA Download PDFInfo
- Publication number
- KR19990064373A KR19990064373A KR1019980023810A KR19980023810A KR19990064373A KR 19990064373 A KR19990064373 A KR 19990064373A KR 1019980023810 A KR1019980023810 A KR 1019980023810A KR 19980023810 A KR19980023810 A KR 19980023810A KR 19990064373 A KR19990064373 A KR 19990064373A
- Authority
- KR
- South Korea
- Prior art keywords
- dna
- product
- present
- plasmid
- vector
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A44—HABERDASHERY; JEWELLERY
- A44C—PERSONAL ADORNMENTS, e.g. JEWELLERY; COINS
- A44C25/00—Miscellaneous fancy ware for personal wear, e.g. pendants, crosses, crucifixes, charms
- A44C25/001—Pendants
- A44C25/002—Pendants forming a container, e.g. for pictures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B44—DECORATIVE ARTS
- B44C—PRODUCING DECORATIVE EFFECTS; MOSAICS; TARSIA WORK; PAPERHANGING
- B44C5/00—Processes for producing special ornamental bodies
- B44C5/005—Processes for producing special ornamental bodies comprising inserts
-
- A—HUMAN NECESSITIES
- A44—HABERDASHERY; JEWELLERY
- A44B—BUTTONS, PINS, BUCKLES, SLIDE FASTENERS, OR THE LIKE
- A44B15/00—Key-rings
- A44B15/005—Fobs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
본 발명은 DNA를 추출하여 증폭한 후, 벡터에 클로닝하여 얻어진 플라스미드를 대량 분리하여 염색한 뒤 제품에 주입하는 것을 특징으로 하는 제품의 장식방법에 관한 것이다.The present invention relates to a method for decorating a product, characterized in that after extracting and amplifying DNA, the plasmid obtained by cloning into a vector is separated and stained in bulk and then injected into a product.
본 발명의 방법에 의하면 공여자만의 차별화된 캐릭터를 독특하게 표현할 수 있는 DNA로 장식된 제품을 얻을 수 있으며, 얻어진 제품은 보관상 용이하고, 미관이 유려하므로, 공여자의 캐릭터를 휴대하고자 하는 수요가 있는 다양한 제품, 예를 들어 열쇠고리, 음반, 문구 등을 장식하는데 본 발명의 방법을 유용하게 이용할 수 있다.According to the method of the present invention, it is possible to obtain a product decorated with DNA that can uniquely express a donor's differentiated character. The method of the present invention can be usefully used to decorate various products, such as key chains, records, stationery, and the like.
Description
본 발명은 DNA를 이용한 제품의 장식방법에 관한 것으로, 더욱 상세하게는 DNA를 추출하여 증폭한 후, 벡터에 클로닝하여 얻어진 플라스미드를 대량 분리하여 염색한 뒤 제품에 주입하는 것을 특징으로 하는 제품의 장식방법에 관한 것이다.The present invention relates to a method for decorating a product using DNA, and more particularly, to extract and amplify DNA, and then to a large number of plasmids obtained by cloning in a vector, which is stained and then injected into the product. It is about a method.
DNA는 세포핵 염색체의 기초 물질로서 각종 유전 정보를 보유하고 있어 의학분야 및 생명공학분야의 중요한 연구 대상으로 검토, 분석되고 있다. 그러나, 생명체로부터 분리되는 DNA는 소량에 불과하므로 이를 연구하기 위해서는 DNA 중 어떤 특정 서열을 다량 증폭하는 과정이 필요하며, 이에 이용 가능한 다양한 방법 및 장치들이 공지되어 있다.(Mulis, K. et al, Specific enzymatic amplification of DNAin vitro: the polymerase chain reaction. Cold spring Harbor Symp. Quant. Biol. 51, 263-273, 1986; 미합중국 특허 제4,683,195호, 제4,683202호, 제4,889,818호, 대한민국 특허 제126,231호 등)DNA is a basic substance of cell nucleus chromosome and has various genetic information. However, since only a small amount of DNA is separated from living organisms, it is necessary to amplify a large amount of a specific sequence of DNA in order to study it, and various methods and apparatuses that can be used are known (Mulis, K. et al, Specific enzymatic amplification of DNA in vitro : the polymerase chain reaction.Cold spring Harbor Symp.Quant. Biol. 51, 263-273, 1986; United States Patent Nos. 4,683,195, 4,683202, 4,889,818, and Korean Patent No. 126,231 Arc, etc.)
본 발명자들은 DNA가 그 공여자만의 차별화된 캐릭터를 독특하게 표현할 수 있는 물질임을 감안하여, 공여자의 캐릭터를 휴대하고자 하는 수요가 있는 다양한 제품에 증폭된 DNA를 함유하는 장식문양을 삽입함으로써, 보관이 용이하고, 미관이 유려한 DNA로 장식된 제품을 얻을 수 있다는 사실을 알아내어 본 발명을 완성하게 되었다.The inventors consider that DNA is a material capable of uniquely expressing the donor's differentiated character, and thus, by inserting a decorative pattern containing amplified DNA into various products in demand to carry the donor's character, it is easy to store. The present invention was completed by finding out that a product decorated with beautiful DNA can be obtained.
따라서, 본 발명의 목적은 DNA 증폭 및 HLA DNA 밴드 스트립 기술을 이용하여 악세사리, 음반, 문구류 등의 여러 제품에, 공여자의 특징을 나타내는 DNA를 주입하여 장식하는 방법을 제공하는 데에 있다.Accordingly, it is an object of the present invention to provide a method of injecting and decorating a DNA indicative of a donor to various products such as accessories, records, stationery, etc. using DNA amplification and HLA DNA band stripping techniques.
도 1은 본 발명의 일 실시형태에 따라 DNA 플라스미드 및 HLA DNA 밴드 스트립를 함유하도록 장식된 열쇠고리 제품을 나타낸 것이다.1 shows a keychain product decorated to contain a DNA plasmid and a HLA DNA band strip in accordance with one embodiment of the present invention.
도 2는 본 발명의 다른 실시 형태에 따라 장식된 목걸이 제품을 나타낸 것이다.2 shows a necklace product decorated according to another embodiment of the present invention.
도 3은 본 발명의 또 다른 실시 형태에 따라 장식된 목걸이 제품을 나타낸 것이다.3 shows a necklace product decorated according to another embodiment of the present invention.
※도면의 주요 부분에 대한 부호의 설명※ Explanation of code for main part of drawing
1: 열쇠 고리 2: HLA DNA 밴드 스트립1: key chain 2: HLA DNA band strip
3: DNA 공여자의 사진 4: 실크인쇄된 싸인3: photo of DNA donor 4: silk-printed autograph
5: DNA 용액 6: 물5: DNA solution 6: water
10, 20: 목걸이10, 20: necklace
이를 위하여 본 발명에 의하면, DNA를 추출하여 증폭한 후, 벡터에 클로닝하여 얻어진 플라스미드를 대량 분리하여 염색한 후 제품에 주입하는 것을 특징으로 하는 제품의 장식방법이 제공된다.To this end, according to the present invention, a method of decorating a product, characterized in that the DNA is extracted and amplified, and the plasmid obtained by cloning into a vector is separated and dyed in bulk and then injected into a product.
본 발명의 다른 실시형태에서는, 혈액 시료로부터 DNA를 추출하여 사용하며, 증폭된 DNA의 일부를 사용하여 HLA DNA 밴드 스트립을 제조하여 제품에 부착하고, 나머지 증폭 DNA를 벡터에 클로닝하여 얻어진 플라스미드를 염색한 후 제품에 주입하는 것을 특징으로 하는 제품의 장식방법이 제공된다.In another embodiment of the present invention, DNA is extracted from a blood sample and a portion of the amplified DNA is used to prepare an HLA DNA band strip and attach it to the product, and to stain the plasmid obtained by cloning the remaining amplified DNA into a vector. After the product is decorated, it is provided with a decoration method of the product.
본 발명에 사용되는 DNA 시료는 혈액, 체모, 피부조직, 손톱 등 신체의 어떤 부분, 또는 신체에서 얻을 수 있는 모든 가검물을 사용할 수 있다.As the DNA sample used in the present invention, any part of the body such as blood, hair, skin tissue, nails, or any specimen obtained from the body can be used.
시료 종류에 따라 방법상 약간의 차이가 있기는 하나, 모두 공지의 방법으로 DNA를 추출, 회수할 수 있다.(Buffone 등, Isolation of DNA from biological specimens without extraction with phenol. Cln. Chem. 31: 164-165, 1985) 혈액을 예로 들면, EDTA 혈액과 RBC 용해 완충용액과의 혼합물에 원심분리 등을 실시하고, 얻어진 잔사를 Chelex 수지와 혼합, 끓여 추출할 수 있다.Although there are some differences in the method depending on the type of sample, all can be extracted and recovered by known methods (Buffone et al., Isolation of DNA from biological specimens without extraction with phenol.Cln.Chem. 31: 164). 165, 1985) For example, the mixture of EDTA blood and RBC lysis buffer may be centrifuged, and the obtained residue may be mixed with Bolex resin, boiled and extracted.
DNA 증폭은 예를 들어 Mulis 등의 방법을 이용하여, 통상의 PCR 기기를 사용하여 실시할 수 있다. 보다 간단하게 유전자 키트(Inno-lipa사제 HLA Genotyping kit)를 사용하여 증폭할 수도 있다.DNA amplification can be performed using a conventional PCR instrument, for example, using a method such as Mulis. More simply, the gene kit (HLA Genotyping kit available from Inno-lipa) can be amplified.
증폭된 DNA는 TA 클로닝 키트(Invitrogen사 제품)을 사용하여 벡터에 클로닝할 수 있다. 클로닝 키트를 사용하지 않는 경우에는 다음과 같이 벡터 클로닝할 수 있다. 증폭된 DNA에 T4 DNA 폴리머라아제를 넣되 dNTP는 넣지 않고 15분간 반응시키고, dNTP는 넣고 15분간 반응시켜, DNA를 양 끝에 돌출되어 있는 T와 A(한 쪽에 하나씩 돌출)가 제거된 블런트 말단(blunt end)으로 만든다. 클로닝할 벡터도 SmaI와 같은 블런트 말단 제한 효소로 절단하고, 블런트 말단이 된 상기 DNA를 T4 DNA 리가아제를 사용하여 벡터에 결찰(ligation)하여 클로닝한다.The amplified DNA can be cloned into the vector using a TA cloning kit (Invitrogen). If you do not use a cloning kit, you can vector clone as follows: T4 DNA polymerase was added to the amplified DNA for 15 minutes without dNTP, and dNTP was reacted for 15 minutes to remove the blunt ends from which T and A (one on each side) protruding DNA were removed. blunt end). The vector to be cloned is also cleaved with a blunt end restriction enzyme such as SmaI, and the blunt end DNA is cloned by ligation to the vector using T4 DNA ligase.
클로닝된 플라스미드는 적당한 숙주세포에 도입하여 대량 분리할 수 있다. 숙주세포로는 E.콜라이 HB 101, E.콜라이 CSH 41 등을 사용할 수 있으며, 본 발명에 의한 플라스미드가 도입되어 형질 전환된 숙주세포를 LB(Luria broth) 배지 등에서 배양하여, Birnboin Doly (1979) 등의 방법에 따라 플라스미드를 추출, 분리할 수 있다.The cloned plasmid can be introduced into a suitable host cell and separated in large quantities. As host cells, E. coli HB 101, E. coli CSH 41 and the like can be used. The host cells transformed with the plasmid according to the present invention were introduced and cultured in LB (Luria broth) medium, and Birnboin Doly (1979). The plasmid can be extracted and separated in accordance with such a method.
대량 분리된 플라스미드는 브로모페놀블루, 페놀 레드 등의 염색시약을 첨가하거나 또는 글리세롤과 염색 시약을 첨가하여 장식하고자 하는 제품에 주입할 수 있다.The mass separated plasmid may be injected into a product to be decorated by adding a dyeing reagent such as bromophenol blue or phenol red, or by adding glycerol and a dyeing reagent.
DNA 추출 시료로 혈액샘플을 사용한 경우에는 HLA DNA 밴드 스트립을 제작하여, DNA가 주입된 제품의 표면 등에 부착할 수 있다. HLA DNA 밴드 스트립은 DNA 공여자만이 갖는 특징을 가시화하는 효과가 있다.In the case of using a blood sample as a DNA extraction sample, an HLA DNA band strip may be prepared and attached to the surface of a product into which DNA is injected. HLA DNA band strips have the effect of visualizing features unique to DNA donors.
HLA DNA 밴드 스트립은 다음과 같이 제작할 수 있다. 혈액 시료에서 추출된 DNA를 PCR을 통하여 증폭하고 예를 들어, Inno-lipa사에서 제공된 키트 스트립과 반응시킨다. 스트립에는 백혈구의 여러 부위를 확인할 수 있는 프로브가 일정한 간격으로 흡착되어 있어, 추출된 DNA와 일치하는 프로브는 DNA와 결합한다. 추출된 DNA와 반응하여 발색되는 시약을 첨가하면 결합된 부위가 색을 나타내게 되는데 사람마다 특이적인 DNA 염기 서열을 가지므로 다양한 형태의 스트립 밴드를 볼 수 있다.HLA DNA band strips can be constructed as follows. DNA extracted from blood samples is amplified by PCR and reacted with a kit strip provided by, for example, Inno-lipa. On the strip, probes that can identify different regions of white blood cells are adsorbed at regular intervals, so that probes that match the extracted DNA bind to the DNA. When the reagent is developed by reacting with the extracted DNA, the bound site becomes colored. Since each person has a specific DNA sequence, various band bands can be seen.
본 발명에 의하여 장식할 수 있는 제품에는 열쇠고리, 인형, 장식품, 목걸이, 귀걸이 등의 악세사리류 외에도 CD 포장박스, 필기도구, 필통 등의 문구류, 가방류, 신발류 등이 포함되나 이에 한정되지 않는다. 염색된 DNA의 장식효과를 증대시기키 위해서 DNA가 삽입되는 부분은 투명 재질을 사용하는 것이 바람직하며, 투명체에 의하여 형성되는 모양은 다양하게 선택할 수 있다.Products that can be decorated by the present invention include, but are not limited to, accessories such as key chains, dolls, ornaments, necklaces, earrings, such as stationery, bags, footwear, such as CD packaging boxes, writing instruments, pencil case. In order to increase the decorative effect of the dyed DNA, it is preferable to use a transparent material for the DNA insertion portion, and the shape formed by the transparent body can be variously selected.
DNA가 주입되어 장식된 제품에는 DNA가 함유되어 있음을 특징적으로 나타내는 일반적인 DNA의 이중나선 그림, DNA 공여자의 싸인 및 사진 등을 추가할 수도 있다.Products infused with DNA can be added with a double helix picture of a typical DNA, a signature of a DNA donor, and a picture of the DNA that characterizes the presence of DNA.
(실시예)(Example)
DNA의 추출·입수DNA extraction and acquisition
DNA의 공여자로부터 EDTA 코팅된 튜브에 전혈 3ml를 채취하여, 2-8℃로 냉장보관하였다.3 ml of whole blood was collected in an EDTA coated tube from a donor of DNA and refrigerated at 2-8 ° C.
멸균된 1.5ml 마이크로원심분리 튜브에 EDTA 혈액 50㎕, RBC 용해 완충용액을 가하고 잘 혼합하였다. 13,000rpm으로 1분간 원심분리하여 침사를 얻었다. 침사를 500㎕ 멸균 증류수로 세척하였다. RBC가 잔존하는 경우, 원심분리 및 세척 과정을 반복하였다. 세척된 침사에 Chelex 수지 200㎕를 넣고 잘 풀어 주었다. 10분간 끓인 후, 원심분리하여 상층액 10㎕를 DNA 증폭에 사용하였다. 10분간 끓인 후 냉장 상태로 식힌 다음 다시 10분간 끓이는 것을 반복할 수 있다.50 µl of EDTA blood and RBC lysis buffer were added to a sterile 1.5 ml microcentrifuge tube and mixed well. Centrifugation was performed for 1 minute at 13,000 rpm to obtain acupuncture. The needles were washed with 500 μl sterile distilled water. If RBC remained, the centrifugation and washing were repeated. 200 μl of Chelex resin was added to the washed salivary solution, and the solution was well released. After boiling for 10 minutes, the mixture was centrifuged and 10 µl of the supernatant was used for DNA amplification. After boiling for 10 minutes, cool in a chilled condition and then boil again for 10 minutes.
DNA 증폭DNA amplification
하기의 조성으로 증폭 혼합물을 준비하였고, 하기 조건에서 PCR 기기(Inno-lipa사 제품, HLA Genotyping kit)를 사용하여 DNA를 증폭시켰다.An amplification mixture was prepared with the following composition, and DNA was amplified using a PCR instrument (Inno-lipa, HLA Genotyping kit) under the following conditions.
I. 증폭 혼합물(총 50㎕)I. Amplification mixture (50 μl total)
II. PCR 조건II. PCR conditions
전처리Pretreatment
1. HLA DNA 밴드 스트립 제조1. HLA DNA Band Strip Manufacturing
증폭된 DNA로부터 HLA 키트(Inno-lipa사제)를 사용하여 HLA DNA 밴드 스트립을 제조하였다.HLA DNA band strips were prepared from the amplified DNA using an HLA kit (manufactured by Inno-lipa).
2. DNA 삽입물 제조2. DNA Insert Preparation
증폭과정에서 증폭된 DNA를 TA 클로닝 키트(Invitrogen사제)를 이용하여, 벡터에 클로닝한 후, 클로닝된 플라스미드를 대량 분리하였다. 대량분리시 균주로는 E. 콜라이 HB101를 사용하고, 균주 배양 조건은 37℃에서 18시간동안 진탕배양(shaking incubation)하였다. 일반적으로 사용되는 LB(Luria broth) 배지를 제조하여 일차 HB101 세균을 하룻밤 배양한 뒤, 500ml LB에 1/100 희석한 배양 세균을 다시 접종하여 배양하였다. 플라스미드 분리는 다음과 같이 실시하였다(Birnboin Doly, 1979). 플라스미드 DNA를 갖는 대장균(대장균 HB101)을 37℃에서 18시간동안 배양하고, 플라스미드 분리시약 SolI[50mM 글루코오스, 25mM Tris·Cl(pH 8.0), 10mM EDTA(pH 8.0)], SolⅡ[0.2N NaOH, 1% SDS], SolⅢ[5M 아세트산 칼륨 60ml, 빙초산(glacial acetic acid) 11.5ml, H2O 28.5ml]를 차례로 첨가한 후, 부드럽게 혼합하고, 14000rpm으로 10분간 원심분리 후, 상층액을 모아 페놀:클로로포름(1:1) 용액을 동량 섞고 강하게 혼합하였다. 이를 다시 원심분리 및 상층액 수집하고, 상기 과정을 2 내지 3회 반복하여 깨끗한 상층액만을 취한 후, 에탄올(100%)을 2배(체적) 첨가하여 10분간 냉동고에 정치하였다. 이를 원심분리 한 후, 상층액을 제거하고 건조시켜, 70% 에탄올 1ml로 1회 세척하고, 건조하여 TE 완충액으로 DNA를 용해시키고 냉동고에 보관 뒤 사용하였다.The DNA amplified in the amplification process was cloned into a vector using a TA cloning kit (manufactured by Invitrogen), and the cloned plasmid was isolated in large quantities. E. coli HB101 was used as a strain during mass separation, and the strain culture conditions were shaken incubated for 18 hours at 37 ° C. In general, LB (Luria broth) medium was used to culture primary HB101 bacteria overnight, and then cultured by inoculating 1/100 diluted cultured bacteria in 500ml LB. Plasmid separation was performed as follows (Birnboin Doly, 1979). Escherichia coli (E. coli HB101) having plasmid DNA was incubated at 37 DEG C for 18 hours, and plasmid separation reagent SolI [50 mM glucose, 25 mM Tris-Cl (pH 8.0)], 10 mM EDTA (pH 8.0)], Sol II [0.2N NaOH, 1% SDS], SolIII [60M potassium acetate 60ml, glacial acetic acid 11.5ml, H 2 O 28.5ml] were added sequentially, mixed gently, centrifuged at 14000 rpm for 10 minutes, and the supernatant was collected and phenol. : Chloroform (1: 1) solution was mixed in the same amount and mixed vigorously. This was again centrifuged and the supernatant was collected, and the procedure was repeated 2-3 times to take only the clean supernatant, and then ethanol (100%) was added twice (volume) and left in the freezer for 10 minutes. After centrifugation, the supernatant was removed and dried, washed once with 1 ml of 70% ethanol, and dried to dissolve DNA in TE buffer and used after storage in a freezer.
분리된 DNA에 글리세롤, 브로모페놀블루를 첨가하였다.Glycerol and bromophenol blue were added to the isolated DNA.
후처리After treatment
안쪽 부분이 오목한 지름 5cm, 두께 0.5cm의 원형 아크릴판 두 개(한 개는 액체 주입부 및 고리 연결부를 갖는다)를 통상의 방법으로 제조하여, 도 1에서와 같은 배치로 HLA DNA 밴드 스트립(측면 부착), DNA 공여자의 사진 및 실크인쇄된 싸인을 넣은 후, 아크릴 양면을 스프레이 강력 접착하였다. 액체 주입부를 통해 전처리 과정에서 제조된 DNA 용액을 가득 채우고, 이를 막았다. 양면이 같은 모양으로 되도록 제작하며, 전체 두께는 1cm로 하였다. 고리 연결부에 열쇠고리를 끼워 악세사리를 완성하였다.Two circular acrylic plates 5 cm in diameter and 0.5 cm thick (one having a liquid inlet and a ring connection) with concave inner portions were prepared in a conventional manner, and the HLA DNA band strips (sides) as shown in FIG. Attached), photographs of DNA donors and silk-printed signs, and then spray strongly adhered to both sides of the acrylic. Through the liquid inlet, the DNA solution prepared during the pretreatment was filled up and blocked. Both sides were made to have the same shape, and the overall thickness was 1 cm. A key ring is attached to the hook to complete the accessory.
본 발명의 방법에 의하면 악세사리의 새로운 형태를 제시함과 동시에 공여자만의 차별화된 캐릭터를 표현할 수 있으며, 보관이 용이하고, 미관이 유려한 DNA 함유 악세사리 제품을 얻을 수 있다.According to the method of the present invention, it is possible to present a new form of the accessory and at the same time express the donor's differentiated character, and to obtain a DNA-containing accessory product that is easy to store and has a beautiful appearance.
Claims (4)
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019980023810A KR19990064373A (en) | 1998-06-24 | 1998-06-24 | Decoration method of product using DNA |
| PCT/KR1999/000335 WO1999067358A2 (en) | 1998-06-24 | 1999-06-24 | Method of preparing objects containing dna |
| CN99807627A CN1306402A (en) | 1998-06-24 | 1999-06-24 | Method of preparing objects contg. DNA |
| AU46555/99A AU4655599A (en) | 1998-06-24 | 1999-06-24 | Method of preparing objects containing dna |
| KR1019990023961A KR100272933B1 (en) | 1998-06-24 | 1999-06-24 | Method of preparing objects containing dna |
| JP2000556003A JP2002518040A (en) | 1998-06-24 | 1999-06-24 | Method for producing DNA-containing products |
| KR1020000034717A KR20000063098A (en) | 1998-06-24 | 2000-06-23 | Method of preparing objects containing dna |
| US09/987,005 US20020055118A1 (en) | 1998-06-24 | 2001-11-13 | Method of preparing objects containing DNA |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019980023810A KR19990064373A (en) | 1998-06-24 | 1998-06-24 | Decoration method of product using DNA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR19990064373A true KR19990064373A (en) | 1999-08-05 |
Family
ID=19540571
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019980023810A KR19990064373A (en) | 1998-06-24 | 1998-06-24 | Decoration method of product using DNA |
Country Status (5)
| Country | Link |
|---|---|
| JP (1) | JP2002518040A (en) |
| KR (1) | KR19990064373A (en) |
| CN (1) | CN1306402A (en) |
| AU (1) | AU4655599A (en) |
| WO (1) | WO1999067358A2 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20010018878A (en) * | 1999-08-23 | 2001-03-15 | 김정호 | Method for embodying personal DNA structures in an article and its article |
| KR200233589Y1 (en) * | 2000-12-05 | 2001-09-25 | 이종인 | Character products for individual identification using gene information |
| KR100330911B1 (en) * | 1999-03-16 | 2002-04-03 | 이의근 | Fancy goods decorated by human protein band strip |
| KR100330910B1 (en) * | 1999-03-16 | 2002-04-03 | 홍승현 | Fancy goods decorated by human protein |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4719952B2 (en) * | 2000-01-21 | 2011-07-06 | 友田 珠緒 | Birth memorial |
| DE10065089A1 (en) * | 2000-12-21 | 2002-07-18 | Olek Alexander | Jewelry item with genetic fingerprint |
| AU2002255333A1 (en) * | 2001-05-09 | 2002-11-18 | Ebara Corporation | Affinity reaction probe detection/analysis chips and detection system and apparatus using the same |
| ES2204237B1 (en) * | 2001-05-25 | 2005-04-16 | Manuel Gonzalez Perez | CONTAINER FOR STORAGE OF GENETIC MATERIAL. |
| US20050045063A1 (en) * | 2001-11-02 | 2005-03-03 | Matthias Niggemann | Marking solution for counterfeit-resistant identification of a valuable object, marking produced by the marking solution and method for marking a valuable object |
| CN102511980A (en) * | 2011-12-31 | 2012-06-27 | 福建师范大学 | Preparation method for preparing mementoes storing genetic information of human bodies in direct coating manner |
| CN102511979A (en) * | 2011-12-31 | 2012-06-27 | 福建师范大学 | Preparation method for preparing mementoes storing genetic information of human bodies in indirect coating manner |
| WO2015050899A1 (en) * | 2013-10-02 | 2015-04-09 | Fabric Media | Products enhanced with synthetic genetic material |
| AT516857B1 (en) * | 2015-05-06 | 2016-09-15 | Guger Forschungs Gmbh | finger ring |
| CN110074517A (en) * | 2019-04-28 | 2019-08-02 | 白城师范学院 | A kind of phytochrome and the key combined chain of genome and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2690691B1 (en) * | 1992-04-29 | 1999-02-12 | Bio Merieux | METHOD OF AMPLIFYING RNA REQUIRING A SINGLE HANDLING STAGE. |
| WO1995030749A1 (en) * | 1994-05-10 | 1995-11-16 | Stargene, Inc. | Exhibiting device |
| DE29715707U1 (en) * | 1997-09-02 | 1998-01-02 | Huber, Matthias C., Dr.rer.nat., 79232 March | Genomic DNA (deoxyribonucleic acid) molecules fixed in transparent plastic, undissolved, visible to the human eye in their entirety, prepared from plant or animal cell material |
-
1998
- 1998-06-24 KR KR1019980023810A patent/KR19990064373A/en active Search and Examination
-
1999
- 1999-06-24 AU AU46555/99A patent/AU4655599A/en not_active Abandoned
- 1999-06-24 JP JP2000556003A patent/JP2002518040A/en active Pending
- 1999-06-24 CN CN99807627A patent/CN1306402A/en active Pending
- 1999-06-24 WO PCT/KR1999/000335 patent/WO1999067358A2/en active Application Filing
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100330911B1 (en) * | 1999-03-16 | 2002-04-03 | 이의근 | Fancy goods decorated by human protein band strip |
| KR100330910B1 (en) * | 1999-03-16 | 2002-04-03 | 홍승현 | Fancy goods decorated by human protein |
| KR20010018878A (en) * | 1999-08-23 | 2001-03-15 | 김정호 | Method for embodying personal DNA structures in an article and its article |
| KR200233589Y1 (en) * | 2000-12-05 | 2001-09-25 | 이종인 | Character products for individual identification using gene information |
Also Published As
| Publication number | Publication date |
|---|---|
| AU4655599A (en) | 2000-01-10 |
| WO1999067358A3 (en) | 2000-03-30 |
| JP2002518040A (en) | 2002-06-25 |
| WO1999067358A2 (en) | 1999-12-29 |
| CN1306402A (en) | 2001-08-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR19990064373A (en) | Decoration method of product using DNA | |
| Robinson et al. | Effects of antineoplastic drugs on the post-strand-passage DNA cleavage/religation equilibrium of topoisomerase II | |
| Crick | On protein synthesis | |
| Noren et al. | Construction of high-complexity combinatorial phage display peptide libraries | |
| ES2321243T3 (en) | NUCLEIC ACID LIGANDS THAT BIND DNA POLYMERASES AND INHIBIT THEM. | |
| Sigal et al. | A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases | |
| Keller et al. | Thermococcus alcaliphilus sp. nov., a new hyperthermophilic archaeum growing on polysulfide at alkaline pH | |
| CN109563514A (en) | Use the high specific genome editor of the guidance RNA of chemical modification | |
| Harada et al. | Specific cleavage of tRNA by nuclease S1 | |
| CN110312800A (en) | The method for handling sample of nucleic acid | |
| JP2001523098A (en) | Method for the production of ASLV reverse transcriptase consisting of multiple subunits | |
| US20020055118A1 (en) | Method of preparing objects containing DNA | |
| CN102839181A (en) | Nucleic acid aptamer sequence of gastric cancer cells, and uses thereof | |
| CN101397550A (en) | Mutant firefly luciferase | |
| KR100272933B1 (en) | Method of preparing objects containing dna | |
| KR100330910B1 (en) | Fancy goods decorated by human protein | |
| KR20000063098A (en) | Method of preparing objects containing dna | |
| Marx | Oncogenes Reach a Milestone: In the 20 years since genes with oncogenic potential were discovered in cells, the research has grown explosively, extending far beyond cancer causation to normal cell biology | |
| CN101712992B (en) | Chlamys nobilis molecular marker with orange shell color and identification method thereof and kit | |
| CN104745584B (en) | One kind is for disturbing arrenotokous nucleic acid molecules of Macrobrachium rosenbergii and preparation method thereof | |
| Kim et al. | Characterization of the endosymbiont of a deep-sea bivalve, Calyptogena soyoae | |
| CN113502344B (en) | Nucleic acid molecule primer, method and kit for identifying Boletus viscosus | |
| Lapeyrie et al. | Some factors influencing viability of ectomycorrhizal fungal inoculum | |
| Reichenberger et al. | Cloning, purification and characterization of DNA polymerase β from Xenopus laevis: Studies on its potential role in DNA‐end joining | |
| KR100330911B1 (en) | Fancy goods decorated by human protein band strip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A201 | Request for examination | ||
| N231 | Notification of change of applicant | ||
| N231 | Notification of change of applicant | ||
| G15R | Request for early opening | ||
| N231 | Notification of change of applicant |