KR100330911B1 - Fancy goods decorated by human protein band strip - Google Patents

Abstract

The present invention relates to an ornament that is decorated using a band strip of protein, and specifically, isolates a band strip obtained from a protein obtained from a donor or a protein obtained from a donor's gene by genetic engineering method, in particular a blood protein. The present invention relates to ornaments decorated with ornaments, accessories, cards, accessories for mobile phones, records, stationery, and the like by dying the band strips obtained by inserting or directly attaching them to the product. Various products decorated with protein band strips according to the present invention are easy to store and have a beautiful appearance, and also contain a characteristic image of the donor, thereby maximizing the image that the owner is with the donor. It can be widely applied to various products requiring the expression of characters.

Description

Fancy goods decorated by human protein band strip}

The present invention relates to an ornament using a protein band strip, and specifically, after staining a band strip obtained from a protein obtained from a donor or a protein obtained from a donor's gene by genetic engineering method, in particular, a band strip obtained by separating blood proteins The present invention relates to an ornament inserted or attached to a product to be decorated.

In order to meet the demands of those who want to feel and share the image of the public star in close proximity, various products are decorated by inserting photos or signs to characterize the character of the popular star. In addition, in order to highlight the specificity and make the appearance more colorful, the product is decorated using a holographic technique. Until now, however, there have been no examples of using proteins, particularly those obtained from donors, to decorate products.

Proteins are the components that make up the body of an organism. Specifically, they are the main components of muscles, skin, and bones. They are responsible for the formation of skeletons, store energy, and are the main components of various constituents in the blood. It also serves to transport Moreover, various chemical reactions are constantly occurring in cells, which are necessary for the synthesis of all the substances that make up the organism and for supplying all the energy necessary for the activity of the organism. All these chemical reactions must be carried out in the right amount and at the right place at the right time, and the protein is the main component of the enzyme responsible for this regulation and catalyst. In addition, protein is an organic substance that is very important in maintaining life, such as forming an antibody against an antigen from outside to play an immune response, transmit nerve signals, and make up substances and hormones involved in the growth and differentiation of cells.

Specifically, blood is divided into plasma and cellular portion. Plasma is a physiological component of blood, that is, a liquid component excluding blood cells, and is a transparent, light yellow neutral liquid. If anticoagulant is added to the blood and centrifuged or left at a low temperature (about 0 ° C), the blood cells settle downward and are separated into plasma and blood cells. Plasma accounts for about 50-60% of the total blood, and plasma again contains water, plasma proteins, inorganic salt ions such as sodium, chlorine, potassium, calcium and magnesium, sugars, lipids such as cholesterol and lecithin, amino acids and hormones. It is divided into vitamins, gases dissolved in plasma. Plasma proteins mainly include albumin, globulin, and fibrinogen, and the various plasma components have different physical and chemical properties such as size, molecular weight, affinity and charge for a particular molecule. It is different and can be separated by a variety of suitable physical and chemical methods. Blood cells also consist mainly of red blood cells, which supply oxygen, carbon dioxide and nutrients to different parts of the body, various types of white blood cells against external viruses, and platelets responsible for blood coagulation in the event of bleeding. Doing. The total amount and composition of plasma and the state of blood cell components change significantly according to the physical condition of each individual such as disease, which is useful for diagnosing or detecting the disease. Therefore, plasma and blood cell components extracted from each donor and their isolation One aspect can be said to contain the characteristic characteristics of the donor.

Electrophoresis is used as a method for separating proteins. Electrophoresis refers to a phenomenon in which colloidal particles move toward one electrode when an electrode is placed in a colloidal solution and a DC voltage is applied. Electrophoresis occurs because the colloidal particles are charged, and the rate of movement of the particles varies with the electrokinetic potential difference at the interface of the particles, and when the electrolyte is adsorbed, the magnitude of the potential difference changes with the concentration or type of electrolyte in the solution. Affected accordingly. The rate of movement also varies with the size and shape of the particles and the type and concentration of ions in the solution. Therefore, even though the various properties of the colloidal particles are the same, if any one of them is different, the particles can be separated by electrophoresis, mainly used for separation and analysis of polymers such as proteins. In particular, electrophoresis is mainly performed on a gel, and since the gel itself plays a role of a molecular sieve at the same time, the separation efficiency is higher, and the protein can be characterized from several separated bands. . The most widely used method of gel electrophoresis is especially SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), since the SDS anion binds about two amino acid residues, so the total charge of the denatured protein bound to SDS depends on its protein mass. Nearly proportional, the protein can be separated very efficiently by mass.

These proteins, on the other hand, are synthesized as directed by the organism's genes. In addition to natural synthesis, proteins have recently been artificially synthesized using genetic engineering. Genetic engineering is a technique for intentionally changing existing organisms or new types of organisms. Recombinant DNA technology, cell fusion technology, and nuclear replacement technology can be used to artificially process genes of living organisms to obtain a large amount of substances necessary for humans at low cost. Gene recombination is to cut a gene synthesizing a specific protein with a restriction enzyme and put it in a plasmid of E. coli to perform protein synthesis. For example, human insulin, growth hormone, interferon, etc. are produced in E. coli.

Recombinant gene technology basically consists of two processes. The first is to link the DNA fragments to appropriate gene vectors (such as plasmids and bacteriophages) that have independent replication capabilities. Since DNA fragments are incapable of self-replicating, they must be linked to small replicons (plasmids or viruses) so that they can enter new host cells and function as genes, allowing them to self-replicate in that host cell. . The second step is to inject the recombinant DNA into the host cell to express the genetic information. The specific transcription process is different for microorganisms and higher organisms, which usually make up complementary DNA (cDNA) that is reversed from mRNA and inject it into the microorganism to express traits. First, extract mRNA from specific cells, and when the synthesis of the desired protein is confirmed, make the cDNA using reverse transcriptase and treat it with alkali to remove RNA, and then use cDNA as a template Then, DNA-dependent polymerase is used to synthesize cDNA of two strands. Unlike the DNA of the original gene (genonaic DNA), the obtained cDNA does not have introns. Thus, cDNAs containing almost pure genetic information are synthesized and introduced into bacteria, such as plasmids, and introduced into bacteria. To get the desired protein.

As such, the specificity or individuality of every organism is determined by the gene of the individual, whether between species or individuals, so that the many kinds of proteins produced by this gene reflect the specificity of the individual. Thus, the morphological and functional differences between two individuals are the differences in the types of proteins in their bodies.

By the way, although it is a gene that contains genetic information about each individual, it is through protein that individual's unique trait is expressed by gene. Furthermore, as mentioned above, proteins are not only directly constituents of each individual entity, but they also play all roles in the activities of organisms, and each has a different ability to identify each molecule. Since specificity is conferred, it can be said that the characteristic of the protein donor is outstanding. Among them, as described above, the blood is the most basic driving force for living organisms, and the protein in the blood and the blood itself have a greater meaning for each individual. Thus, proteins in the human body, particularly proteins contained in the blood, exhibit unique characteristics of the donor and may have a differentiated identification effect on the donor. Moreover, the characteristic effect of these proteins is superior to the images that can be represented by the above signs or photographs of donors.

Accordingly, the present inventors have realized that the protein in the human body exhibits a unique feature that only the donor has and can have a psychological effect of being with the donor. Therefore, there is a demand for carrying a donor's character, especially a band strip of the protein. The present invention has been accomplished by finding that it can effectively decorate various products.

It is an object of the present invention to provide an ornament that is decorated by inserting or attaching a band strip of processed protein to a variety of products, such as accessories, cards, records, and stationery, so that the donor can exhibit distinctive features.

Figure 1 shows the SDS-PAGE results of the degraded protein,

2 shows a card product decorated to contain a protein band strip according to an embodiment of the invention.

※ Explanation of codes for main parts of drawing

1: card 2: protein band strip

3: photo or signature of donor 4: analysis of donor protein

5: serial number

In order to achieve the above object, the present invention provides an ornament in which a band strip of a donor protein is inserted or attached.

Specifically, the present invention provides an ornament using a protein band strip prepared by separating a protein taken from a donor to prepare a band strip, dyeing and inserting or attaching the product to a product. At this time, the protein may include those obtained from tissues or secretions of the body such as blood, saliva, skin tissue of the donor. In addition, the protein may include amplifying a gene of a donor, cloning it into a plasmid vector, and culturing the transformant obtained by transducing Escherichia coli, and then obtaining the protein band strip. The present invention provides an ornament using a protein band strip, which comprises inserting or attaching the obtained image to a product.

The protein may be separated by one or more of conventional protein separation methods and injected or attached to the product. More specifically, donor proteins may be used for gel electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), dialysis, gel-filtration chromatography, ion exchange chromatography. one or more of ion exchange chromatography, affinity chromatography, centrifugation, salting out, isoelectric focusing, enzyme-linked immunosorbent assay (ELISA), and western blotting It may be separated by a method, but is not limited thereto.

The present invention provides an ornament using a protein band strip produced by SDS-PAGE of the protein.

In addition, the protein band strip is obtained by separating the protein from a number of proteins, or provides a decoration using a protein band strip, characterized in that obtained by breaking down the protein in several parts using a protease.

In another aspect, the present invention provides an ornament using a protein that dyes some or all of the protein to inject or attach to the product. More specifically, the dyeing may be performed using a dyeing solution of bromophenol bule or Coomassie brilliant blue R-250 or by silver staining. It is not limited.

The protein band strip of the present invention can be inserted or mounted on an ornament by placing it on the surface of the product and attaching it using a conventional transparent sticker or a transparent adhesive such as epoxy.

Ornaments using the protein band strips include jewelry, bracelets, necklaces, headbands, ornaments, dolls, accessories, key chains, card holders, cell phone accessories, video tapes, records and cases, bags, pencil cases, writing instruments, notebooks. There are stationery, etc., and products using proteins can also be used in genealogy table of relatives, but is not limited thereto.

Hereinafter, the present invention will be described in detail.

The protein may be one obtained from a donor, or may include amplified donor gene, cloned into a plasmid vector, and cultured transformant obtained from the culture. Specifically, cDAN is synthesized by separating template RNA from donor blood, and cDNA is amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). The amplified cDNA is cloned into plasmids such as pMAL-P2 or pUC 19 which are commonly used, and E. coli is transformed with the plasmid into which the gene is inserted. The transformed Escherichia coli is cultured in large quantities and expressed to obtain the desired protein.

Proteins can be extracted and separated according to the characteristics of each component, for example, by known methods depending on the size or mass, the affinity for a particular molecule, the amount of charge, and the like. These proteins can be separated by gel electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), dialysis, gel-filtration chromatography, ion exchange chromatography (ion) exchange chromatography, affinity chromatography, centrifugation, salting out, isoelectric focusing, enzyme-linked immunosorbent assay (ELISA) and western blotting can be used. . In particular, the protein is preferably obtained by using a band strip using SDS-PAGE.

In addition, the protein may be separated from itself among several proteins to prepare a band strip, or a protease may be used to prepare a band strip of protein that has been broken down into various parts.

Proteins can be isolated and stained in order to produce excellent visual effects when inserted into the product, in which case Coomassie blue and bromophenol blue are stained. , Dyes such as phenol red, or silver staining methods (Wray et. Al., Anal. Biochem. 1981 , 118, 197-203) may be used, but are not limited thereto. Do not.

The protein can be inserted or attached to the product using its own band strip obtained separately by the above methods. Specifically, the band strip of protein may be positioned inside the ornament, or may be attached so as to be located on the outer surface of the ornament. In the latter case, the band strip may be attached to the surface of the product by using a transparent sticker such as a transparent sticker or epoxy.

In order to enhance the visual effect when decorating the product with the protein band strip separated and dyed by the method of the present invention, the portion to which the protein band strip is attached is preferably made of a transparent material. It can be manufactured in various forms according to the characteristics and purposes. In addition, for stable storage of the inserted protein band strip, the transparent body in which the protein band strip is inserted must be finished in a completely sealed state. In addition, the description of the protein using a method such as sticker printing, PVC printing, or silk printing, to indicate that the product is decorated with a protein band strip by the above method to insert a specific donor-specific protein, Pictures, signs of protein donors, and photographs may be further included.

Products that can be decorated using protein band strips by the method of the present invention include accessories such as bracelets, necklaces, headbands, hairpins, dolls, bookmarks, accessories, key rings, bag cards, cell phone accessories, video tape , Stationery and cases, bags, pencil cases, writing instruments, stationery such as notebooks, but is not limited to these. The donor protein band strips can also be used for applications such as genealogy between family and relatives using the method of the present invention.

Since the present invention decorates a product using protein band strips in which the genetic information unique to each individual is expressed in surface area and visually, the present invention is directed to a person who owns a product decorated with protein, in particular, using blood. It has the effect of planting the image that it is with the protein donor. Moreover, the characteristic and vertical effect of these protein band strips is far superior to the images shown in products containing commercially available donor signs and photographs.

Hereinafter, the present invention will be described in more detail with reference to Examples.

However, the following examples are illustrative of the present invention, and the content of the present invention is not limited by the examples.

Example 1 Blood Collection and Separation

In order to prevent coagulation of the blood from the protein donor, 20 μg of blood was collected using a syringe to which anticoagulant was added, and the blood was centrifuged at 1,000 rpm for 3 minutes to separate the supernatant and the precipitate. Plasma contains several components, so take about 1 의 of the separated plasma (supernatant) and add trichloroacetic acid to 10% to acidify the pH of the plasma to precipitate the protein. Again, the protein precipitate obtained by centrifugation at 10,000 rpm for 15 minutes was dissolved again. As described above, blood cells and plasma components were separated from the blood, and the serum components of the plasma were used to prepare a band strip.

Example 2 Collection of Blood and Isolation of Template RNA

Blood was collected from the protein donor using a syringe containing anticoagulant to prevent blood clotting. The blood was placed in a tube and 100 μl of denaturation solution [4M guanidium thiocyanate, 25 mM sodium citrate (pH 7.0), 0.5% sarcosyl, 0.1M 2-mercaptoethanol] was waited until the protein was digested. Then add 10 μl of 2 M sodium acetate (pH 4) and mix well, add 100 μl of saturated water-saturated phenol (approximately pH 4.5), mix well, and then add chloroform: iso 20 μl of propyl alcohol (chloroform: isoamyl alcohol = 49: 1) mixed solution was mixed well. Thereafter, the mixed solution was placed in an ice tube for 15 minutes, and then centrifuged at 15,000 rpm for 15 minutes at 4 ° C.

After centrifugation, the supernatant was transferred to a new tube, and 240 µl of high-purity ethanol was mixed well, and left at -20 ° C for 15 minutes. Then centrifuged once more under the same conditions as above, the supernatant was discarded and the precipitate was washed with 80% ethanol. The precipitate was dried, dissolved in 100 μl of DEPC treated water and stored at −20 ° C.

Example 3 Gene Amplification

(1) Synthesis of cDNA

First, cDNA was synthesized to perform RT-PCR.

6.0 μl of 5 × reverse transcriptase buffer, 1.5 μl of 2.5 mM dNTPs, 1.0 μl of 500 ng / μl DTT, 1.0 μl of 100 mM DTT (dithreitol) and 19.5 μl of RNA and DEPC treated distilled water prepared in Example 2 30 μl of the reaction mixture was prepared by adding 1.0 μl of 200 units / μL MMLV reverse transcriptase, followed by incubation at 42 ° C. for 30 minutes, and then at 75 ° C. for 30 minutes.

(2) cDNA amplification

The cDNA generated primarily in the reverse transcription reaction was amplified by the following method.

First, 5.0 μl of the mixed solution prepared by the procedure of (1), 5.0 μl of 10 × buffer, 4.0 μl of 2.5 mM dNTP, 25 pmol / μl upstream primer 1.0 μl, 25 pmol / μl downstream primer A total solution of 50 μl was prepared by adding 1.0 μl, 2.5 unit / μl Taq polymerase 1.0 μl and distilled water 33.0 μl. The polymerase chain reaction (PCR) was carried out with the reaction mixture, and PCR conditions were 3 minutes at 95 ° C, 1 minute at 94 ° C, 1 minute 30 seconds at 55 ° C, and 1 minute at 72 ° C, and It was 3 minutes at 72 degreeC.

The desired P53 gene (about 2.95 kb) was obtained by PCR amplification, and the band was confirmed by electrophoresis on a 0.7% agarose gel.

Example 4 Gene Cloning and Transformation

The gene amplified by PCR in Example 3 was cloned into a commonly used pMAL-P2 (NEB) plasmid vector.

Plasmid pMAL-P2 was cleaved with restriction enzymes Sph I and BamH I and cloned into the gene. The restriction enzymes used above were added at the same time and reacted for 30 minutes to 2 hours at 30 to 40 ° C. to decompose and cleavage the plasmid. The impurities were extracted and removed by phenol and chloroform and precipitated and purified by ethanol. To the recovered plasmid fragment, the purified donor gene was added in an amount of 2 to 10 times and reacted in the presence of DNA binding enzyme for 4 to 40 hours. E. coli JM103 was transformed using the added plasmid, and only the transformed E. coli strain was isolated from the selection medium and used in subsequent experiments.

On the other hand, the pMAL-p2 plasmid vector has a maltose binding protein secreted into the periplasmic space of E. coli. By doing so, the proteins could be easily separated.

The transformed E. coli strains were inoculated in LB medium and cultured in aeration using a fermentor. Before entering the main culture, seed cultures were carried out, and the growth stages of the strains were the same, and O.D. Incubated to 600 = 0.42.

Example 5 Collection and Separation of Proteins

(1) protein collection

The culture cultured in Example 4 was collected by centrifugation at 4,000 g for 10 minutes, and then washed once with Tris buffer. Thereafter, the membranes in the cell membrane were collected by bursting the strain membrane in the culture medium using osmotic pressure. Specific methods are as follows.

The culture solution was dissolved in 30M Tris buffer and 20% sucrose solution, centrifuged to release the collected strains again, and then mixed well by adding 0.5 M EDTA until the final concentration was 1 mM. After shaking for 10 minutes and again centrifuged at 8000g at 4 ℃, the supernatant was discarded, strains were collected, and 10ml of 5 mM MgSO ₄ cooled with ice was added to the precipitate, followed by shaking for 10 minutes on ice water. Then centrifuged again under the same conditions as above to obtain the desired protein in the supernatant.

(2) protein isolation

Since proteins collected by osmotic pressure are mixed with heterologous proteins, desired proteins were separated by affinity chromatography as follows.

A column of 2.5 cm in diameter and 10 cm in length was filled with an amylose resin, wherein the amount of the resin was such that 3 mg per ml of the substance passing through the column could be bound. The column was washed with 8 times the volume of buffer, and the protein solution collected by the osmotic process of (1) was injected into the column at a rate of 1 ml / min. Thereafter, the column was washed with a 12-fold volume of buffer solution, and passed through a buffer solution and 10 mM maltose mixed solution to separate only the desired protein bound to the resin in the column.

Proteins were separated and collected by 3 ml in 15 tubes, and the amount of separated protein was measured from the absorbance at 280 nm by UV spectrophotometer, and the purity was confirmed by SDS-PAGE.

On the other hand, purely separated protein was digested by treatment at 37 ° C. for 2 hours or more with a proteinase (peptidase) so that several bands appeared when separated by SDS-PAGE (see FIG. 1 ).

Example 6 Preparation of Protein Band Strips

A certain amount of the serum component isolated in Example 1 or the protein obtained in Example 5 was separated on a gel using SDS-PAGE (Laemmli, UK, Nature, 1970, 227, 680-685), which is a conventional protein separation method. .

The gel was prepared and used in the composition shown in Table 1, and the total amount was 100 ml, and then air was removed and a certain amount was carefully poured into the glass plate groove to polymerize.

Composition of gel Gel ingredient ratio 8% Tris-glycine SDS-PAGE gel Distilled Water 30% Acrylamide 1.5M Tris (pH 8.8) 10% SDS 10% APSTEMED 46 ml 27 ml 25 ml 1 ml 1 ml 0.06 ml 100 ml total stacking gel Distilled Water 30% Acrylamide 1M Tris (pH 6.8) 10% SDS 10% APSTEMED 68 ml 17 ml 12.5 ml 1 ml 1 ml 0.1 ml total 100 ml

The protein sample is loaded on the gel prepared as described above in an amount that can be separated, and then subjected to electrophoresis at a constant current of about 10 mA per surface area of the gel to separate the protein sample. Band strips were prepared.

Example 7 Staining of Protein Band Strips

The band strip obtained by separating the protein in Example 6 is bromophenol blue, Coomassie brilliant blue R-250, or silver staining method (Wray et. Al. , Anal. Biochem. 1981 , 118, 197-203).

(1) Bromophenol Blue Dyeing

Water and methanol were mixed at a ratio of 1: 1 to prepare 90 ml, and then 10 ml of acetic acid was added thereto to prepare a decolorizing solution. Staining solution was prepared by adding a bromophenol blue reagent to a total concentration of 0.16%. The gel was electrophoresed and soaked in a dyeing solution and shaken for 2 hours or more, and then, the gel was decolorized by shaking the gel slowly while replacing the decolorizing solution several times. By this stain the protein was stained blue.

(2) komash blue dyeing

Water and methanol were mixed at a ratio of 1: 1 to prepare 90 ml, and then 10 ml of acetic acid was added thereto to prepare a decolorizing solution. The dyeing solution was prepared by adding 0.25 g of Coomassie brilliant blue R-250 reagent thereto. The gel was electrophoresed and soaked in a dyeing solution and shaken for 2 hours or more, and then, the gel was decolorized by shaking the gel slowly while replacing the decolorizing solution several times. By this stain the protein was stained blue.

(3) silver staining

Staining by silver staining method (Wray et. Al., Anal. Biochem. 1981 , 118, 197-203) was carried out with the solution of Table 2. First, the gel was stained with solution 1 for 5 minutes and solution 2 for 5 minutes, followed by solution 3 again for 30 minutes. Then, the gel was stained for 5 minutes with solution 4, three times for 5 minutes with solution 5, and then stained for 5 minutes with solution 6, and finally, the gel was stained with solution 7 for 5 minutes. Then, the gel was decolorized with the decolorizing solution prepared in (1) or (2), and the protein was stained brown by this dyeing.

Solution of silver staining Solution number Furtherance Solution 1 50% Methanol + 12% Acetic Acid Solution 2 10% Methanol + 5% Acetic Acid Solution 3 0.1% K ₂ Cr ₂ O ₇ + 20 μl / 100 mL HNO ₃ Solution 4 0.2% AgNO ₃ Solution 5 3% sodium carbonate Solution 6 3% Sodium Carbonate + 50 μl 37% Formaldehyde Solution 7 3% acetic acid

Example 8 Preparation of Decorated Product Using Protein Band Strip (1)

In one preferred embodiment of the present invention for the card-like product was prepared a product in which the band strip of protein is inserted or attached as follows (see Figure 2 ).

In order to include the donor's signature, photograph, serial number, or a description of the protein, the grooves are first made to have a width of 5 mm, a length of 3 cm, and a depth of 1 mm at a predetermined position of a card manufactured according to a conventional method. The band strip of protein prepared in Example 7 was cut to size and completely sealed to the card with a conventional transparent adhesive such as a transparent sticker or epoxy.

Example 9 Preparation of Decorated Product Using Protein Band Strip (2)

In one preferred embodiment of the present invention for the card-like product was prepared a product in which the band strip of protein is inserted or attached as follows (see Figure 2 ).

The protein band strip produced in Example 7 was scanned using a conventional drum scan method to obtain an image of the protein band strip. The card was then prepared by including an image of the band strip in a card manufactured according to a conventional method such that the donor's signature, photograph, serial number or protein description was included.

As described above, the present invention provides a method of using a band strip obtained by separating a new type of material, a protein that is characteristic of a donor, which is not used in the past, in decorating various products. A product decorated with a protein band strip according to the invention can be given the characteristic value of a protein donor to have the effect of maximizing the image of the owner of the product with the donor.

Claims (10)

Ornaments using protein band strips made by inserting or attaching a band strip of donor proteins to a product. The ornament using a protein band strip according to claim 1, wherein the protein is obtained from tissues or secretions of the body such as donor blood, saliva, and skin tissue. The ornament according to claim 1, wherein the protein is obtained from the culture by culturing the transformant obtained by amplifying a donor gene, cloning it into a plasmid vector and transducing E. coli. 2. An ornament using a protein band strip according to claim 1, wherein an image obtained by scanning the protein band strip in a conventional manner is inserted or attached to a product. 2. The donor protein of claim 1, wherein the donor's protein comprises: gel electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), dialysis, gel-filtration chromatography, ion exchange chromatography One of ion exchange chromatography, affinity chromatography, centrifugation, salting out, isoelectric focusing, enzyme-linked immunosorbent assay (ELISA), and western blotting Ornament using a protein band strip, characterized in that separated by the above method. The ornament using a protein band strip according to claim 1, wherein the protein band strip is made by SDS-PAGE. According to claim 1, wherein the protein band strip is obtained by separating the protein from a number of proteins, or ornaments using a protein band strip, characterized in that obtained by breaking down the protein in several parts using a protease. The method of claim 1, wherein the dyeing is performed using a dyeing solution of bromophenol blue or Coomassie brilliant blue R-250 or by silver staining. Ornaments with protein band strips. The ornament using a protein band strip according to claim 1, wherein the protein band strip is placed on the surface of the product and attached using a normal transparent sticker or a transparent adhesive such as epoxy. The method of claim 1, wherein the ornaments are jewelry, bracelets, necklaces, headbands, ornaments, dolls, accessories, keys, card holders, cell phone accessories, video tapes, records and cases, bags, pencil cases, writing instruments, notebooks, etc. Ornaments using a protein band strip, characterized in that the stationery stationery and relative lineage chart.