KR19990047377A - Beverages containing extracts of circumference as main ingredients and preparation method thereof - Google Patents

Abstract

The present invention relates to a beverage containing the extract of the situation (Phellinus linteus) and a method for manufacturing the same, the health beverage containing the perinus linteus extract prepared by the present invention is almost indistinguishable from the usual beverage of taste, Since no precipitate is formed, it is possible to suppress the occurrence of cancer by using a commercial beverage as if drinking.

Description

Beverage containing the extract of Phellinus linteus as a main ingredient and its manufacturing method

The present invention relates to a beverage containing the extract of Phellinus linteus and a method for producing the same.

Basidiomycete has long been used as a traditional Chinese medicine. In particular, anti-cancer polysaccharides produced by basidiomycete have little side effects and are very safe in terms of toxicity, and have been known to have an excellent anti-cancer effect by enhancing the function of the immune system. Methods of using such basidiomycetes include collecting or cultivating fruiting bodies and separating the mycelium from the fruiting bodies, and then cultivating the mycelium to use the mycelium. The method of collecting and using wild fruiting bodies has problems of depletion of resources and destruction of ecosystems, and thus have been used by artificial cultivation. However, even in the case of artificial cultivation, the growth rate is very slow and there are problems such as bacterial infection.

In order to solve this problem, the present inventors have conducted a long research to develop a method for culturing mycelia of Perinus linteus, and have applied for a patent and obtained a patent or pending. [Patent Publication (Korea Patent Publication No. 92-1367 (polysaccharides) and a method of their production), Patent Publication (Korea Patent Publication No. 92-1194) (Culturing method of Perinus linteus mycelium), Patent Publication No. 97-9150 (Artificial liquid culture and anticancer activity of Felinus linteus mycelium Substance preparation method), patent publication (Korea Patent Laid Open Publication No. 97-15743) (anti-cancer immunoactive polysaccharide isolated from Perinus linteus and its preparation method), patent publication (Korea Patent Laid Open Lublication No. 97-1531) (A novel Felinus linteus strain producing anticancer immunoactive substances).

Extracts of these situation mushrooms (Perinus linteus) can be further purified to obtain an immune polysaccharide (Korea Patent Publication No. 92-1367; Korea Patent Laid Open Publication No. 97-15743), all of which will be used in the present invention Can be.

Extracts and polysaccharides of Perinus linteus mycelium prepared by the above method (hereinafter referred to as "perinus linteus extract") is digestive organ cancer, liver cancer, anti-immunity enhancement, detoxification, etc. by chemotherapy combined with cancer resection It has been found that the pharmacological action of has already been used as medicines and powders as a medicine.

Moreover, people living in modern times are exposed to various pollutants and carcinogens, which causes a high rate of cancer. Once cancer develops, prevention is most desirable because not only is the treatment difficult, but the patients suffer very much and the survival rate is not high.

The present inventors have conducted a long research to develop a health beverage containing Perinus linteus extract as a main component. The use of such a healthy drink containing Perinus linteus extract as a main component can suppress cancer development, and its taste is also almost indistinguishable from ordinary drinks, and no precipitate is produced.

Types of beverages that may be used in the present invention include juices, alcoholic beverages, cola, milk, coffee, tea and other conventional alcoholic beverages and alcoholic beverages.

As juices, orange juice, tomato juice, apple juice, pear juice, and other conventional juices can be used. In addition, such juices include not only juice made from 100% stock solution but also diluted juice prepared by adding water thereto. In addition, flavor beverages to which conventional flavors are added may also be used in the present invention. Such flavor beverages are alcoholic beverages prepared by adding orange flavor, grape flavor, lemon flavor, strawberry flavor and the like.

As the liquor, it is preferable to use beer, wine, cider, lemon liquor and other liquor having a low content of alcohol.

Felinus linteus extract, which may be added to conventional beverages, contains 1.0 to 5,000 mg per 100 ml of beverage. Preferably from 5 mg to 500 mg per 100 ml of the beverage.

The health beverage of the present invention is prepared by adding Fellinus linteus extract to a conventional beverage and sterilizing it if necessary.

Next, the present invention will be described in more detail as Examples and Experimental Examples.

Example 1

Culture of Felinus linteus KCTC 0082BP strain

To cultivate the Felinus linteus strain, a medium containing 50 g of glucose, 10 g of peptone, 10 g of yeast extract, and 0.5 g of dibasic potassium phosphate was used per liter of medium. Production medium includes 10 g of soluble starch, 50 g of glucose, 5 g of waste yeast extract, 10 g of corn steriquois, 0.5 g of dipotassium phosphate, 0.3 g of magnesium sulfate, and MnCl ₂ , FeSO ₄ , ZnSO ₄ as trace elements. The loaded medium was used. Both seed and production media were used after adjusting the pH to 5.0 before sterilization.

After sterilizing the 500ml Erlenmeyer flask containing 50ml of the above medium for 20 minutes at 121 ° C, peel off the Plinus linteus KCTC 0082PB hyphae on Gaza-glucose-agar agar medium with a small amount of medium, and inoculate 3 at 28 ° C. Shake culture was carried out daily to prepare a primary seed culture solution. Then, sterilize the jar fermenter containing 2 liters of seed medium at 121 ° C for 20 minutes, homogenize the primary seed culture solution, inoculate 50ml, incubate at 28 ° C for 3 days, and then as secondary seed culture. It was.

Subsequently, 500 liters of fermented liquor containing 300 liters of production medium was sterilized in advance and then stored in 4 liters of the secondary seed culture solution and pre-cultured at 28 ° C. At this time, the culture conditions were aeration 300 liters / minute, the agitation was 100-200rpm, and the sterilization of the fermenter of the capacity containing 3000 liters of production medium before sterilization, transfer all the cultures aseptically 3000 liters / minute, Stirring was incubated for 3 days at 100-200 rpm. By carrying out the mycelium culture solution in a continuous manner as described above, it is possible to obtain a mycelia in large quantities within 3 days of inoculation, in which a mycelium having a total weight of 25 g / l was obtained.

Example 2

Extraction of Anticancer Immune Boosting Substances

The mycelia obtained in Example 1 were extracted in the following manner and separated and purified. That is, 100 g of the obtained mycelium was added to 500 ml of distilled water at 90-100 ° C., and extracted by boiling water twice for 2 hours. The mycelium cake was removed and only the hot water extract was recovered. The hot water extract is concentrated in vacuo to 100 ml, and then added to three volumes of 95% ethanol and allowed to stand overnight, followed by centrifugation at 3000 rpm for 30 minutes. The extract thus obtained was dialyzed for about 3 days using a dialysis membrane which was dissolved in 100 ml of water and was capable of dialysis of a substance having a molecular weight of 8000 or less, and then lyophilized at -70 ° C to obtain 3 g of a polymer extract containing polysaccharide.

Example 3

Extraction of Crude Polysaccharides

10-20 times the amount of water was added to the mycelium culture obtained in Example 1, and hot water was extracted at 50-100 ° C. for 1-2 hours, filtered by centrifugation, concentrated with a thin film pressure reducer, lyophilized, or the concentrated solution. Silver is dried by spray dryer to obtain crude polysaccharide.

Experimental Example 1

Acute Toxicity Test

Perineus linteus extract obtained in Example 2 was intraperitoneally administered to 10 4 week old ICR mice at a concentration of 10,000 mg / kg and observed for 14 days. No abnormalities were found in all 10 animals.

Experimental Example 2

Anticancer activity

The anticancer activity was examined for the sarcoma 180 of rat sarcoma cancer transplanted into the ICR mice 4 weeks old born with the Perinus linteus extract obtained in Example 2. That is, Sarcoma 180 cells were transplanted intraperitoneally with ICR-based mouse cells and incubated for 7 days, and then 1 × 10 ⁶ cells were implanted under the left groin of the ICR-based mouse. The experimental drug was dissolved in physiological saline and sterilized with 0.45ug of bacterial filtration membrane and administered intraperitoneally once a day at 100mg / kg once every 24 hours after cancer transplantation. After 30 days of cancer transplantation, solid tumors were extracted and the percentage of tumor inhibition was calculated. As a result, administration of the extract of Felinus linteus showed an antitumor activity of about 71.5%.

[Table 1] Anticancer activity against solid cancer (sarcoma 180)

Perinus linteus extract of the present invention has an excellent effect on digestive cancer (The New Medical Journal (Korea) Vol. 39, No. 11 November 1996), has an immunosuppressive effect (Journal of Korean Cancer Association Vol. 29, NO 3, 1997), and has an excellent effect on B 16 (Int. J. Immunopharmac., Vol. 18, No. 5, pp. 295-303).

Therefore, the extract of Felinus linteus of the present invention is almost non-toxic and has an anticancer effect, and thus can be used as a health food that is effective in preventing and treating cancer.

Next, the present invention will be described in more detail as formulation examples.

Formulation Example 1

Perinus linteus extract in 100 ml of orange juice (hereinafter referred to as "PL Ex.")

10mg, 20mg, 30mg, 40mg, 50mg, 100mg each put, stirred, dissolved, sterilized and filled into a 100ml bottle and sterilized by a conventional method to prepare the orange juice of the present invention and the original orange juice color and taste and Precipitates (PPT) were compared.

The results were as follows.

Formulation Example 2

10 ml, 20 mg, 30 mg, 40 mg, 50 mg, 100 mg each of Perinus linteus extract (PL Ex.) Was added to 100 ml of milk, dissolved by stirring well, and sterilized and filled in a 100 ml bottle and sterilized by a conventional method. Was prepared and the taste, color and precipitation of the original milk were compared.

The results were as follows.

Formulation Example 3

Add 10 mg, 20 mg, 30 mg, 40 mg, 50 mg, and 100 mg of Perinus linteus extract (PL Ex.) To the collagen solution, respectively, dilute with purified water and adjust the sugar to normal 100 ml. Cola was prepared by injecting carbon dioxide, and the color, taste and precipitation production of the original coke were compared.

The results were as follows.

Formulation Example 4

10ml, 20mg, 30mg, 40mg, 50mg, 100mg each of Perinus linteus extract (PL Ex.) Was added to 100ml of beer, and stirred well to dissolve, and then injected with carbonic acid gas. After filling the bottle of the dose was compared the color and taste and sedimentation of the original beer.

The results were as follows.

Formulation Example 5

After 10%, 20mg, 30mg, 40mg, 50mg, 100mg each of Perrinus linteus extract was added to 100ml of wine with 12% alcohol content, and the mixture was dissolved by stirring to compare the color, taste and precipitation of the original wine.

The results were as follows.

Formulation Example 6

In 100ml of milk, 5mg and 1000mg of Perinus linteus extract (PL Ex.), Respectively, were added by stirring well, dissolved, sterilized, filled into a 100ml bottle, and sterilized by a conventional method to prepare the milk of the present invention. Taste and color and precipitation production of the was compared.

The results were as follows.

Formulation Example 7

Add 1,000 mg of coffee to 80 ml of boiling water, and add 10 mg, 20 mg, 30 mg, 40 mg, 50 mg, and 100 mg of Perinus linteus extract (PLEx.) To each, and stir well to make each black coffee and add no PL Ex. The color and taste of the coffee and the precipitated product were compared.

The results were as follows.

Formulation Example 8

Add 200m of water to 2,000ml of coffee, add 10mg, 20mg, 30mg, 40mg, 50mg, 100mg each of Perinus linteus extract (PL Ex.), Dissolve while stirring well, and fill in 200ml cans to make a normal can coffee After sterilization and sealing by the method, the color, taste and precipitation production of canned coffee without PL Ex. Were compared.

The results were as follows.

Formulation Example 9

10 mg, 20 mg, 30 mg, 40 mg, 50 mg, 100 mg each of Perinus linteus extract (PL Ex.) Was added to 1,000 mg of coffee, mixed with stirring, and stored for 6 months. Next, the resultant was dissolved in 80 ml of boiling water, stirred, and dissolved, and the color, taste, and precipitation production of coffee without PL Ex. Were compared.

The results were as follows.

Formulation Example 10

Add 2,000 mg of green tea extract to 200 ml of boiling water, add 10 mg, 50 mg, 100 mg, 500 mg, 1,000 mg, and 3,000 mg of Perinus linteus extract (PLEx.) To each, stir well, fill in a 200 ml can, and prepare a regular can. The color, taste and precipitation of the original canteen tea without sterilization and sealing and without the addition of PL Ex. Were compared.

The results were as follows.

Formulation Example 11

Perineus linteus extract (PL Ex.) 10mg, 20mg, 30mg, 40mg, 50mg, 100mg each to 1,000mg each of black tea, stir well mixed, sealed and stored for 6 months. Next, it was added to 80 ml of boiling water, stirred and dissolved, and the color, taste, and precipitation production of black tea without PL Ex. Were compared.

The results were as follows.

Perinus linteus extract of the present invention may be prepared by mixing with coffee extract powder or granules, followed by drinking with water, or may be prepared in instant coffee in a state of water.

Perinus linteus extract of the present invention may be prepared by mixing with tea extract powder or granules, followed by drinking with water, or may be prepared as an instant tea in a state of water.

Other juices except orange juice can be prepared by the same method as in Formulation Example 1, and these juices are also within the scope of the present invention.

The health beverage containing the Perinus linteus extract prepared according to the present invention as a main ingredient can hardly be distinguished from ordinary drinks, and no precipitates are formed, so that the commercial drink can be suppressed when used as a normal drink. .

Claims (5)

A health beverage containing Felinus linteus extract prepared by a method of preparing a conventional beverage by mixing an extract of Perlinus linteus with a component commonly used as a beverage. Claim 1, wherein the extract of Perlinus linteus (Phellinus linteus) is added to the health drink containing Felinus linteus extract prepared by the addition of 1.0mg-5,000mg per 100ml health drink. The health drink containing Felinus linteus extract of claim 1, wherein a component commonly used as a beverage is selected from juice, milk, liquor, cola, and coffee. 4. The coffee of claim 3, wherein the coffee is thrown and mixed with coffee extract and Perinus linteus extract and the water is mixed at the time of taking the drink or the coffee extract and the extract of Pelinus linteus and milk, condensed milk, powdered milk, or prim in the water Ferinus linteus extract containing a healthy beverage prepared by the method of preparing a typical instant coffee. Method of preparing a healthy beverage by mixing the extract of Perlinus linteus with the ingredients commonly used as beverages.