WO2008156297A2 - Composition for preventing or treating katzenjammer - Google Patents

Composition for preventing or treating katzenjammer Download PDF

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Publication number
WO2008156297A2
WO2008156297A2 PCT/KR2008/003445 KR2008003445W WO2008156297A2 WO 2008156297 A2 WO2008156297 A2 WO 2008156297A2 KR 2008003445 W KR2008003445 W KR 2008003445W WO 2008156297 A2 WO2008156297 A2 WO 2008156297A2
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Prior art keywords
extract
composition
hangover
glutathione
preventing
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PCT/KR2008/003445
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French (fr)
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WO2008156297A3 (en
Inventor
Se-Hee Hwang
Sae-Gon Kim
Jin-Hee Lee
Nobuaki Ohto
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Cj Cheiljedang Corp.
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Publication of WO2008156297A2 publication Critical patent/WO2008156297A2/en
Publication of WO2008156297A3 publication Critical patent/WO2008156297A3/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/52—Juglandaceae (Walnut family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A61K31/355—Tocopherols, e.g. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/365—Lactones
    • A61K31/375—Ascorbic acid, i.e. vitamin C; Salts thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/06—Aluminium, calcium or magnesium; Compounds thereof, e.g. clay
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K33/00—Medicinal preparations containing inorganic active ingredients
    • A61K33/24—Heavy metals; Compounds thereof
    • A61K33/30—Zinc; Compounds thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/62—Nymphaeaceae (Water-lily family)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/738—Rosa (rose)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/06—Tripeptides
    • A61K38/063—Glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
    • A61P25/32—Alcohol-abuse

Definitions

  • the present invention relates to a composition for preventing or treating a hangover, and more particularly, to a composition for preventing or treating a hangover, the composition comprising all kinds of herb extracts.
  • the ALDH is divided into ALDH type II, which initiates oxidation even in a low concentration of acetaldehyde, and ALDH type I, which does not function in a low concentration of acetaldehyde. Since Eastern people are generally deficient or lack in ALDH (II), the oxidation of acetaldehyde is slower in Eastern people than in Western people. Non-oxidized acetaldehyde and/or ethanol interfere with the normal metabolism, thereby causing various hangover symptoms.
  • the decomposition process of ethanol in the liver includes conversion of ethanol into acetaldehyde through an oxidation reaction. It is known that this decomposition is carried out by three reaction enzyme systems including ADH, microsomal ethanol-oxidiz ing system (MEOS) and catalase (K. Ebihara et al., Agri. Biol. Chem., 52, 1311 , 1988). Recently, many substances capable of reducing the toxicity of ethanol or preventing the expression of toxicity have been studied. In this connection, health-aid foods containing extracts of natural foods or raw herbal materials are being developed (Jung i Han Kim et al., Journal of Korean Society of Agricultural Chemistry and Biotechnology, 38(6), 549-553, 1995).
  • Korean Patent Publication No. 2005-104036 discloses a composition for preventing or treating a hangover, the composition comprising a Rosa roxburghi extract, an Engelgarditia chrysolepsis HANCE extract, and a Nelumbo nucifera extract.
  • the composition comprising these ingredients disclosed in this document effectively decreases the concentration of alcohol in blood and the concentration of acetaldehyde, which is a product of alcohol metabolism, and can be used to prevent and treat a hangover.
  • FIG. 1 is a graph showing test results of change in alcohol concentration in blood when a composition according to an embodiment of the present invention is orally administered to a rat, compared with the cases when compositions prepared in Comparative Example 1 (ADH: combination of herb extracts of Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera), Comparative Example 2 (ADH + water soluble zinc), and Comparative Example 3 (ADH + glutathione-containing yeast extract) are orally administered to rats;
  • ADH combination of herb extracts of Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera
  • Comparative Example 2 ADH + water soluble zinc
  • Comparative Example 3 ADH + glutathione-containing yeast extract
  • FIG. 2 is a graph showing test results of change in acetaldehyde concentration in blood when a composition according to an embodiment of the present invention is orally administered to a rat, compared with the cases when compositions of Comparative Example 1 (ADH), Comparative Example 2 (ADH + water soluble zinc), and Comparative Example 3 (ADH + glutathione-containing yeast extract) are orally administered to rats;
  • Comparative Example 1 ADH
  • Comparative Example 2 ADH + water soluble zinc
  • Comparative Example 3 ADH + glutathione-containing yeast extract
  • FIG. 3 is a graph showing test results of change in alcohol concentration in blood when a composition according to an embodiment of the present invention is orally administered to a human;
  • FIG. 4 is a graph showing test results of change in acetaldehyde concentration in blood when a composition according to an embodiment of the present invention is orally administered to a human.
  • the inventors of the present invention recognized the need to develop medicaments or functional beverages for removing a hangover as described above, studied materials in which metabolism of alcohol can smoothly proceed in order to have an excellent ability to prevent and treat a hangover, and found that by adding a glutathione-containing yeast extract and water soluble zinc to a conventional composition comprising Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera, the concentrations of alcohol and acetaldehyde in blood can be significantly reduced, thus completing the present invention.
  • the present invention provides a composition for preventing or treating a hangover very effectively in comparison with a conventional composition for removing a hangover.
  • a composition for preventing or treating a hangover comprising a glutathione-containing yeast extract, water soluble zinc, a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, and a Nelumbo nucifera extract.
  • a glutathione-containing yeast extract comprising a glutathione-containing yeast extract, water soluble zinc, a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, and a Nelumbo nucifera extract.
  • a composition prepared by adding a glutathione-containing yeast extract and water soluble zinc to a conventional mixture of a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract and a Nelumbo nucifera extract has excellent ability at reducing the concentration of alcohol in blood.
  • the composition has excellent ability at reducing the concentration of acetaldehyde in blood, which is another material inducing a hangover.
  • This mixture which is proven to have the effect of reducing concentrations of alcohol and acetaldehyde, which induce a hangover, can be used as food or medicaments for preventing or treating a hangover.
  • the present invention provides a composition for preventing or treating a hangover, the composition comprising a glutathione-containing yeast extract, water soluble zinc, a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, and a Nelumbo nucifera extract.
  • the composition for preventing or treating a hangover which has the effect of removing a hangover, may comprise 25-45 parts by weight of water soluble zinc, 25-45 parts by weight of the Rosa roxburghi extract, 10-25 parts by weight of the Engelgarditia chrysolpsis HANCE extract, and 10-25 parts by weight of the Nelumbo nucifera extract with respect to 100 parts by weight of the glutathione-containing yeast extract.
  • a weight ratio of the Rosa roxburghi extract to the Engelgarditia chrysolpsis HANCE extract to the Nelumbo nucifera extract may be most preferably 2:1 :1.
  • the glutathione-containing yeast extract denotes an extract of fungus body of
  • glutathione which is a tripeptide composed of three amino acids: glutamate, cysteine and glycine.
  • glutathione-containing yeast extract that contains 5% or greater of glutathione may be used.
  • the water soluble zinc is a mixture of zinc oxide, an emulsifying agent, lecithin, glucose syrup, and dextrin, and can easily be prepared by mixing purchased raw materials by one of ordinary skill in the art or is commercially available.
  • the water soluble zinc can comprise 2.4% or greater of zinc oxide, and can be prepared using the emulsifying agent, for example, glycerol fatty acid ester and lecithin.
  • the Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera extracts may be a powder extract prepared by extracting each herb medicine with water, ethanol, methanol, or a mixed solvent thereof and then spray-dried.
  • the present invention is not limited thereto.
  • the composition for preventing or treating a hangover of the present invention may further include auxiliaries which can further enhance a hangover relief effect, in addition to the primary ingredients, such as vitamins B, C, E or vitamin such as ⁇ -carotin; minerals such as Ca, Mg or Zn; phospholipids such as Lecithin; amino acids such as alanine or taurine; fructose, oligosaccharide, Ganoderma Lucidum, or mixtures thereof.
  • the composition for preventing or treating a hangover can be used as a pharmaceutical composition.
  • a general formulation may be prepared using the composition for preventing or treating a hangover and other pharmaceutical acceptable carriers and additives.
  • the pharmaceutical composition can be orally administered in the form of a solution, a suspension, a powder, a granule, a tablet, a capsule, a pill or an extract, but is not limited thereto.
  • Examples of the pharmaceutically acceptable carrier include at least one selected from the group consisting of a diluent, a lubricant, a binder, a disintegrating agent, a stabilizer, and a preservative.
  • Examples of the additive include at least one selected from the group consisting of a flavoring agent, a coloring material, a sweetening agent, and an acidulant.
  • the diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose or mannitol.
  • the lubricant may be magnesium stearate or talc.
  • the binding agent may be polyvinyl pyrrolidone or hydroxy propyl methyl cellulose.
  • the disintegrating agent may be calcium carboxymethylcellulosde, sodium starch glycolate, polacrilin potassium, or crospovidone.
  • the sweetening agent may be white sugar, fructose, sorbitol, or aspartame.
  • the stabilizer may be sodium carboxymethylcellulose, ⁇ -cyclodextrine, white wax, or Xanthan gum.
  • the preservative may be methyl paraoxybenzoate, propyl paraoxybenzoate or potassium sorbate.
  • additives for improving flavor for example, a natural flavoring agent such as plum, lemon, pineapple or herb flavor; a natural fruit juice; a natural dye such as chlorophyllin or flavonoid; a sweetening component such as fructose, honey, sugar alcohol, or sugar; or an acidulant such as citric acid or sodium citrate may also be included.
  • a natural flavoring agent such as plum, lemon, pineapple or herb flavor
  • a natural fruit juice such as chlorophyllin or flavonoid
  • a sweetening component such as fructose, honey, sugar alcohol, or sugar
  • an acidulant such as citric acid or sodium citrate
  • the composition for preventing or treating a hangover may be administered several times orally having effective ingredients with an amount of 0.3-10 g, preferably 0.7-4.2 g, per day for an adult with a body weight of 60 kg.
  • composition for preventing or treating a hangover can be used as a health aid food.
  • the health aid food can be prepared in the form of a tea, a jelly, an extract, a beverage, etc., comprising extracts of the above medicinal plants as effective ingredients.
  • Various health aid foods of the present invention prevent or remove a hangover without side-effects in human bodies and can be easily administered.
  • the composition for preventing or treating a hangover may be prepared by mixing the above herb extracts.
  • the composition for preventing or treating a hangover may be prepared by adding the glutathione-containing yeast extract and water soluble zinc to each of the extracts of Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera and mixing together.
  • a method of preparing each of the Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera extracts may include: a) extracting each herb with hot water to prepare hot water extracts; b) filtering or centrifuging each hot water extract to separate supernatants; c) concentrating the supernatants under reduced pressure to obtain concentrates of each herb; and d) spray drying each concentrate to obtain powder of each herb extract.
  • the extracting of step b) may be carried out by placing water and each herb in a weight ratio of about 5:1 to 15:1 in an extractor and extracting the herbs at 90-100 0 C for 1 -2 hours.
  • the glutathione-containing yeast extract may be prepared by inoculating baker's yeast into a medium and culturing the yeast, separating strains from the obtained culture, extracting the strains with hot water, and then spray drying the hot water extract.
  • the water soluble zinc may be commercially available, for example, under the name of SUNACTIVE Zn-24 manufactured by TAIYO KAGAKU.
  • the preparation method of the composition for preventing or treating a hangover is not limited thereto, and can be partially modified using extraction methods of herbs known in the art and known methods of preparing the glutathione-containing yeast extract and water soluble zinc.
  • a composition according to the present invention comprising a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, a Nelumbo nucifera extract, a glutathione-containing yeast extract and water soluble zinc exhibits an excellent ability at reducing the concentrations of alcohol and acetaldehyde in blood by comprising the glutathione-containing yeast extract and water soluble zinc, compared with a conventional composition comprising a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, and a Nelumbo nucifera extract.
  • the composition can be used as medicaments or health aid foods that can be effectively used for preventing and treating hangovers.
  • Saccharomyces cerevisae was inoculated in molasses as a medium and cultured.
  • the fungus body was separated from the culture and washed using water, and the washed fungus body was extracted with hot water.
  • the hot water extract was filtered to remove sludge. Then, the hot water extract was sterilized and mixed with dextrin, and the mixture was spray dried to be in the form of a powder.
  • Example 3 Each extract was filtered through a filter paper to obtain 400 g of extract. The extract was left alone for 12 hours and a supernatant was taken. The supernatant was concentrated under reduced pressure to obtain a concentrate containing 50% solids. Each concentrate was spray dried to obtain an extract powder of each of the Rosa roxburghi fruit, the Engelgarditia chrysolepsis HANCE leaf and Nelumbo nucifera.
  • Example 3 Each extract was filtered through a filter paper to obtain 400 g of extract. The extract was left alone for 12 hours and a supernatant was taken. The supernatant was concentrated under reduced pressure to obtain a concentrate containing 50% solids. Each concentrate was spray dried to obtain an extract powder of each of the Rosa roxburghi fruit, the Engelgarditia chrysolepsis HANCE leaf and Nelumbo nucifera.
  • Example 3 Example 3
  • 4-week-old male Wistar rats as an experiment animal, were pre-reared by being allowed to eat solid feed and drink tap water ad libitum for 1 week. After the pre-rearing was finished, the rats were provided with only water and no food for 12 hours before the experiment.
  • the starved rats were orally administered a solution prepared to contain 0.5 g of the glutathione-containing yeast extract powder of Example 1 , 0.2 g of the Rosa roxburghi extract powder of Example 2, 0.1 g of the Engelgarditia chrysolepsis HANCE extract powder of Example 2, 0.1 g of the Nelumbo nucifera extract powder of Example 2 and 0.2 g of water soluble zinc per 7.5 ml of distilled water by using a stomach sonde at a dosage of 7.5 ml/kg. After 30 minutes, ethanol was orally administered to the rats at a dosage of 3 g/kg.
  • a solution in which 0.2 g of the Rosa roxburghi extract powder, 0.1 g of the Engelgarditia chrysolepsis HANCE extract powder, and 0.1 g of the Nelumbo nucifera extract powder were dissolved (Comparative Example 1 ), a solution prepared by additionally dissolving 0.2 g of water soluble zinc in the solution of Comparative Example 1 (Comparative Example 2), and a solution prepared by additionally dissolving 0.5 g of the glutathione-containing yeast extract powder in the solution of Comparative Example 1 (Comparative Example 3) were used.
  • Blood was collected by orbital sinus blood sampling 30 minutes, 1 hour, 3 hours, 6 hours, and 9 hours after administration of ethanol.
  • the collected blood was centrifuged for 10 minutes and serum was separated.
  • the content of ethanol in the serum was analyzed using F-kit ethanol (Roche Diagnostics Corporation).
  • Ethanol is oxidized by the enzyme ADH in the liver to form acetaldehyde.
  • NADH is produced in the presence of NAD + as a coenzyme.
  • the amount of NADH is measured at an absorbance of 340 nm.
  • ⁇ A sample (A2-A1 ) - blank test (A2-A1 )
  • the composition comprising the Rosa roxburghi fruit, the Engelgarditia chrysolepsis HANCE leaf, Nelumbo nucifera (combination of herb extracts), water soluble zinc, and the glutathione-containing yeast extract exhibited an excellent ability at reducing alcohol concentration in blood, compared with the composition of Comparative Example 1 (combination of herb extracts), the composition of Comparative Example 2 (combination of herb extracts + water soluble zinc), and the composition of Comparative Example 3 (combination of herb extracts+ glutathione-containing yeast extract).
  • 4-week-old male Wistar rats as an experiment animal, were pre-reared by being allowed to eat solid feed and drink tap water ad libitum for 1 week. After the pre-rearing was finished, the rats were provided with only water and no food for 12 hours before the experiment.
  • the starved rats were orally administered with a solution prepared to contain 0.5 g of the glutathione-containing yeast extract powder of Example 1 , 0.2 g of the Rosa roxburghi extract powder of Example 2, 0.1 g of the Engelgarditia chrysolepsis HANCE extract powder of Example 2, 0.1 g of the Nelumbo nucifera extract powder of
  • Example 2 and 0.2 g of water soluble zinc per 7.5 ml of distilled water by using a stomach sonde at a dosage of 7.5 ml/kg. After 30 minutes, ethanol was orally administered to the rats at a dosage of 3 g/kg.
  • a solution in which 0.2 g of the Rosa roxburghi extract powder, 0.1 g of the Engelgarditia chrysolepsis HANCE extract powder, and 0.1 g of the Nelumbo nucifera extract powder were dissolved (Comparative Example 1 ), a solution prepared by additionally dissolving 0.2 g of water soluble zinc in the solution of Comparative Example 1 (Comparative Example 2), and a solution prepared by additionally dissolving 0.5 g of the glutathione-containing yeast extract powder in the solution of Comparative Example 1 (Comparative Example 3) were used. Blood was collected by orbital sinus blood sampling 30 minutes, 1 hour, 3 hours, 6 hours, and 9 hours after administration of ethanol.
  • the collected blood was centrifuged at 3000 rpm at 4 ° C for 15 minutes and serum was separated.
  • the content of acetaldehyde in the serum was analyzed using F-kit acetaldehyde (Roche Diagnostics Corporation).
  • F-kit acetaldehyde Roche Diagnostics Corporation
  • the functional principle and kit protocol of the F-kit acetaldehyde are as follows.
  • Acetaldehyde is oxidized by the enzyme ALDH in the liver to form acetic acid.
  • NADH is produced in the presence of NAD + as a coenzyme.
  • the amount of NADH is measured at an absorbance of 340 nm.
  • ⁇ A sample (A2-A1 ) - blank test (A2-A1 )
  • Example 2 the composition of Example 1 comprising the Rosa roxburghi fruit, the Engelgarditia chrysolepsis HANCE leaf, Nelumbo nucifera (combination of herb extracts), water soluble zinc, and the glutathione-containing yeast extract exhibited an excellent ability at reducing acetaldehyde concentration in blood, compared with the composition of Comparative Example 1 (combination of herb extracts), the composition of Comparative Example 2 (combination of herb extracts + water soluble zinc), and the composition of Comparative Example 3 (combination of herb extracts+ glutathione-containing yeast extract).
  • Example 3 Experiments were performed using the composition of Example 3 comprising the raw materials prepared in Examples 1 and 2 in a crossover experiment, a double blind method, and a randomization test.
  • the subjects were allowed to have a washout period between a control group and a test group. All the subjects were called 1 hour before the experiment and provided with the same meal. 2 hours after the meal, the composition of Example 3 was orally administered to the subjects. From 30 minutes after oral administration of the composition of Example 3, the subjects drank 100 g of alcohol for a 1 hour duration.
  • the subjects were provided with 30 g of shrimp chips, as a side dish, called "saewoggang" in Korean and ate them while drinking the alcohol.
  • the subjects were provided with no food for 4 hours after alcohol drinking.
  • 3 ml of blood samples were taken at 0 hour, 1 hour, 2 hours, 5 hours, and 8 hours after alcohol drinking, and then the taken blood was put right away in a vacuum gel tube. Then, the blood was centrifuged at 3000 rpm at 4 0 C for 15 minutes and serum was separated out.
  • the content of ethanol in the serum was analyzed using F-kit ethanol (Roche Diagnostics Corporation).
  • Example 3 of the present invention exhibited an excellent ability at reducing alcohol concentration in blood, compared with the control groups.
  • Example 3 Experiments were performed using the composition of Example 3 comprising the raw materials prepared in Examples 1 and 2 in a crossover experiment, a double blind method, and a randomization test.
  • the subjects were allowed to have a washout period between a control group and a test group. All the subjects were called 1 hour before the experiment and provided with the same meal. 2 hours after the meal, the composition of Example 3 was orally administered to the subjects. From 30 minutes after oral administration of the composition of Example 3, the subjects drank 100 g of alcohol for a 1 hour duration.
  • the subjects were provided with 30 g of shrimp chips, as a side dish, called "saewoggang" in Korean and ate them while drinking the alcohol.
  • the subjects were provided with no food for 4 hours after alcohol drinking.
  • Example 3 of the present invention exhibited an excellent ability at reducing acetaldehyde concentration in blood, compared with the control groups.

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Abstract

Provided is a composition for preventing or treating a hangover, including a glutathione-containing yeast extract, water soluble zinc, a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, and a Nelumbo nucifera extract.

Description

COMPOSITION FOR PREVENTING OR TREATING K ATZEN J AMM ER
TECHNICAL FIELD
The present invention relates to a composition for preventing or treating a hangover, and more particularly, to a composition for preventing or treating a hangover, the composition comprising all kinds of herb extracts.
BACKGROUND ART Since 1980, consumption of alcohol has rapidly increased along with economic development. In 1996, the average amount of daily alcohol consumption per adult in Korea was 6 bottles of beer. Alcohol acts on the central nerve of the brain, making people feel good and alleviating their sufferings. In ancient times, alcohol was used as a basic excipient of all kinds of medicines, and gradually it has come to have a larger role in social gathering. Side effects of drinking alcohol include a heavy feeling in the head, a headache, a pain in one's insides and a parched mouth. These symptoms are often referred to as a hangover. A hangover results from toxic action of ethanol or acetaldehyde in blood. When the toxic action of such material continues for long periods of time, symptoms such as fatigue, abdominal swelling, emesis, etc. are generated. Ethyl alcohol introduced into the body is absorbed by the stomach or the small intestine, thereby entering blood vessels and being transferred to the liver. Liver cells have alcohol dehydrogenase (ADH) which oxidizes alcohol to produce acetaldehyde. Acetaldehyde is metabolized to produce acetic acid by acetaldehyde dehydrogenase (ALDH) in liver cells and transferred to muscles or fat tissue through out the whole body, and is finally decomposed to carbon oxide and water. The ALDH is divided into ALDH type II, which initiates oxidation even in a low concentration of acetaldehyde, and ALDH type I, which does not function in a low concentration of acetaldehyde. Since Eastern people are generally deficient or lack in ALDH (II), the oxidation of acetaldehyde is slower in Eastern people than in Western people. Non-oxidized acetaldehyde and/or ethanol interfere with the normal metabolism, thereby causing various hangover symptoms.
The decomposition process of ethanol in the liver includes conversion of ethanol into acetaldehyde through an oxidation reaction. It is known that this decomposition is carried out by three reaction enzyme systems including ADH, microsomal ethanol-oxidiz ing system (MEOS) and catalase (K. Ebihara et al., Agri. Biol. Chem., 52, 1311 , 1988). Recently, many substances capable of reducing the toxicity of ethanol or preventing the expression of toxicity have been studied. In this connection, health-aid foods containing extracts of natural foods or raw herbal materials are being developed (Jung i Han Kim et al., Journal of Korean Society of Agricultural Chemistry and Biotechnology, 38(6), 549-553, 1995).
In Korea's drinking culture, excessive drinking or frequent drinking has made many people highly interested in medicines or soft-drinks which can remove a hangover. In addition, as lives of consumers improve, interest in functional soft drinks and products has increased, and accordingly, these products are being produced in increasing amounts and types. Thus, there is a need to develop functional soft drinks for removing a hangover.
Korean Patent Publication No. 2005-104036 discloses a composition for preventing or treating a hangover, the composition comprising a Rosa roxburghi extract, an Engelgarditia chrysolepsis HANCE extract, and a Nelumbo nucifera extract. The composition comprising these ingredients disclosed in this document effectively decreases the concentration of alcohol in blood and the concentration of acetaldehyde, which is a product of alcohol metabolism, and can be used to prevent and treat a hangover.
DESCRIPTION OF THE DRAWINGS
FIG. 1 is a graph showing test results of change in alcohol concentration in blood when a composition according to an embodiment of the present invention is orally administered to a rat, compared with the cases when compositions prepared in Comparative Example 1 (ADH: combination of herb extracts of Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera), Comparative Example 2 (ADH + water soluble zinc), and Comparative Example 3 (ADH + glutathione-containing yeast extract) are orally administered to rats; FIG. 2 is a graph showing test results of change in acetaldehyde concentration in blood when a composition according to an embodiment of the present invention is orally administered to a rat, compared with the cases when compositions of Comparative Example 1 (ADH), Comparative Example 2 (ADH + water soluble zinc), and Comparative Example 3 (ADH + glutathione-containing yeast extract) are orally administered to rats;
FIG. 3 is a graph showing test results of change in alcohol concentration in blood when a composition according to an embodiment of the present invention is orally administered to a human; and
FIG. 4 is a graph showing test results of change in acetaldehyde concentration in blood when a composition according to an embodiment of the present invention is orally administered to a human.
DETAILED DESCRIPTION OF THE INVENTION TECHNICAL PROBLEM
The inventors of the present invention recognized the need to develop medicaments or functional beverages for removing a hangover as described above, studied materials in which metabolism of alcohol can smoothly proceed in order to have an excellent ability to prevent and treat a hangover, and found that by adding a glutathione-containing yeast extract and water soluble zinc to a conventional composition comprising Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera, the concentrations of alcohol and acetaldehyde in blood can be significantly reduced, thus completing the present invention. The present invention provides a composition for preventing or treating a hangover very effectively in comparison with a conventional composition for removing a hangover.
TECHNICAL SOLUTION According to an aspect of the present invention, there is provided a composition for preventing or treating a hangover, the composition comprising a glutathione-containing yeast extract, water soluble zinc, a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, and a Nelumbo nucifera extract. Hereinafter, the present invention will be described in geater detail. To find a material having the effect of removing a hangover, the present inventors studied effects of various materials to reduce alcohol concentration and acetaldehyde concentration in blood. As a result, the present inventors found that a composition prepared by adding a glutathione-containing yeast extract and water soluble zinc to a conventional mixture of a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract and a Nelumbo nucifera extract has excellent ability at reducing the concentration of alcohol in blood. In addition, it was found that the composition has excellent ability at reducing the concentration of acetaldehyde in blood, which is another material inducing a hangover. This mixture which is proven to have the effect of reducing concentrations of alcohol and acetaldehyde, which induce a hangover, can be used as food or medicaments for preventing or treating a hangover.
Thus, the present invention provides a composition for preventing or treating a hangover, the composition comprising a glutathione-containing yeast extract, water soluble zinc, a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, and a Nelumbo nucifera extract. The composition for preventing or treating a hangover, which has the effect of removing a hangover, may comprise 25-45 parts by weight of water soluble zinc, 25-45 parts by weight of the Rosa roxburghi extract, 10-25 parts by weight of the Engelgarditia chrysolpsis HANCE extract, and 10-25 parts by weight of the Nelumbo nucifera extract with respect to 100 parts by weight of the glutathione-containing yeast extract. In this case, a more successful effect of preventing and treating a hangover can be obtained. In particular, a weight ratio of the Rosa roxburghi extract to the Engelgarditia chrysolpsis HANCE extract to the Nelumbo nucifera extract may be most preferably 2:1 :1. The glutathione-containing yeast extract denotes an extract of fungus body of
Saccharomyces spp., and contains plentiful amounts of glutathione, which is a tripeptide composed of three amino acids: glutamate, cysteine and glycine. In the composition for preventing or treating a hangover, according to the present invention, the glutathione-containing yeast extract that contains 5% or greater of glutathione may be used.
The water soluble zinc is a mixture of zinc oxide, an emulsifying agent, lecithin, glucose syrup, and dextrin, and can easily be prepared by mixing purchased raw materials by one of ordinary skill in the art or is commercially available. The water soluble zinc can comprise 2.4% or greater of zinc oxide, and can be prepared using the emulsifying agent, for example, glycerol fatty acid ester and lecithin.
The Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera extracts may be a powder extract prepared by extracting each herb medicine with water, ethanol, methanol, or a mixed solvent thereof and then spray-dried. However, the present invention is not limited thereto. The composition for preventing or treating a hangover of the present invention may further include auxiliaries which can further enhance a hangover relief effect, in addition to the primary ingredients, such as vitamins B, C, E or vitamin such as β-carotin; minerals such as Ca, Mg or Zn; phospholipids such as Lecithin; amino acids such as alanine or taurine; fructose, oligosaccharide, Ganoderma Lucidum, or mixtures thereof. The composition for preventing or treating a hangover can be used as a pharmaceutical composition. In this case, a general formulation may be prepared using the composition for preventing or treating a hangover and other pharmaceutical acceptable carriers and additives. The pharmaceutical composition can be orally administered in the form of a solution, a suspension, a powder, a granule, a tablet, a capsule, a pill or an extract, but is not limited thereto.
Examples of the pharmaceutically acceptable carrier include at least one selected from the group consisting of a diluent, a lubricant, a binder, a disintegrating agent, a stabilizer, and a preservative. Examples of the additive include at least one selected from the group consisting of a flavoring agent, a coloring material, a sweetening agent, and an acidulant.
Any pharmaceutically acceptable carrier and additive can be used. In particular, the diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose or mannitol. The lubricant may be magnesium stearate or talc. The binding agent may be polyvinyl pyrrolidone or hydroxy propyl methyl cellulose. The disintegrating agent may be calcium carboxymethylcellulosde, sodium starch glycolate, polacrilin potassium, or crospovidone. The sweetening agent may be white sugar, fructose, sorbitol, or aspartame. The stabilizer may be sodium carboxymethylcellulose, β-cyclodextrine, white wax, or Xanthan gum. The preservative may be methyl paraoxybenzoate, propyl paraoxybenzoate or potassium sorbate.
In addition, additives for improving flavor, for example, a natural flavoring agent such as plum, lemon, pineapple or herb flavor; a natural fruit juice; a natural dye such as chlorophyllin or flavonoid; a sweetening component such as fructose, honey, sugar alcohol, or sugar; or an acidulant such as citric acid or sodium citrate may also be included.
To obtain effects of preventing or treating a hangover, the composition for preventing or treating a hangover may be administered several times orally having effective ingredients with an amount of 0.3-10 g, preferably 0.7-4.2 g, per day for an adult with a body weight of 60 kg.
Also, the composition for preventing or treating a hangover can be used as a health aid food. The health aid food can be prepared in the form of a tea, a jelly, an extract, a beverage, etc., comprising extracts of the above medicinal plants as effective ingredients. Various health aid foods of the present invention prevent or remove a hangover without side-effects in human bodies and can be easily administered.
The composition for preventing or treating a hangover may be prepared by mixing the above herb extracts. In particular, the composition for preventing or treating a hangover may be prepared by adding the glutathione-containing yeast extract and water soluble zinc to each of the extracts of Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera and mixing together.
According to an embodiment of the present invention, a method of preparing each of the Rosa roxburghi, Engelgarditia chrysolpsis HANCE and Nelumbo nucifera extracts may include: a) extracting each herb with hot water to prepare hot water extracts; b) filtering or centrifuging each hot water extract to separate supernatants; c) concentrating the supernatants under reduced pressure to obtain concentrates of each herb; and d) spray drying each concentrate to obtain powder of each herb extract.
The extracting of step b) may be carried out by placing water and each herb in a weight ratio of about 5:1 to 15:1 in an extractor and extracting the herbs at 90-1000C for 1 -2 hours. The glutathione-containing yeast extract may be prepared by inoculating baker's yeast into a medium and culturing the yeast, separating strains from the obtained culture, extracting the strains with hot water, and then spray drying the hot water extract.
The water soluble zinc may be commercially available, for example, under the name of SUNACTIVE Zn-24 manufactured by TAIYO KAGAKU.
However, the preparation method of the composition for preventing or treating a hangover is not limited thereto, and can be partially modified using extraction methods of herbs known in the art and known methods of preparing the glutathione-containing yeast extract and water soluble zinc.
ADVANTAGEOUS EFFECTS
As described above, a composition according to the present invention, comprising a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, a Nelumbo nucifera extract, a glutathione-containing yeast extract and water soluble zinc exhibits an excellent ability at reducing the concentrations of alcohol and acetaldehyde in blood by comprising the glutathione-containing yeast extract and water soluble zinc, compared with a conventional composition comprising a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, and a Nelumbo nucifera extract. Thus, the composition can be used as medicaments or health aid foods that can be effectively used for preventing and treating hangovers.
BEST MODE
The present invention will now be described in greater detail with reference to the following examples. Example 1
Preparation of αlutathione-containinα yeast extract
Saccharomyces cerevisae was inoculated in molasses as a medium and cultured.
The fungus body was separated from the culture and washed using water, and the washed fungus body was extracted with hot water. The hot water extract was filtered to remove sludge. Then, the hot water extract was sterilized and mixed with dextrin, and the mixture was spray dried to be in the form of a powder.
Example 2 Preparation of extract powders of Rosa roxburghi fruit, Engelgarditia chrvsolpsis
HANCE leaf and Nelumbo nucifera
200 g of each of the Rosa roxburghi fruit, the Engelgarditia chrysolepsis HANCE leaf and Nelumbo nucifera was extracted using 2 L of hot water at 950C for 2 hours.
Each extract was filtered through a filter paper to obtain 400 g of extract. The extract was left alone for 12 hours and a supernatant was taken. The supernatant was concentrated under reduced pressure to obtain a concentrate containing 50% solids. Each concentrate was spray dried to obtain an extract powder of each of the Rosa roxburghi fruit, the Engelgarditia chrysolepsis HANCE leaf and Nelumbo nucifera. Example 3
Preparation of composition for preventing and treating hangover
0.002 g of vitamin B1 , 0.002 g of vitamin B2, 1 g of alanine, 5 g of honey, 120 g of fructose, 0.5 g of Ganoderma lucidum, and 20.1 g of taurine were added to 6.6 g of the glutathione-containing yeast extract powder prepared in Example 1 , 2 g of water soluble zinc (SUNACTIVE Zn-24 manufactured by TAIYO KAGAKU)1 2 g of the Rosa roxburghi extract powder prepared in Example 2, 1 g of the Engelgarditia chrysolepsis HANCE extract powder prepared in Example 2 and 1 g of the Nelumbo nucifera extract powder prepared in Example 2. Then, an appropriate amount of water was added thereto to obtain a 1 L composition. The resultant was sterilized by heating at 950C for 10 minutes, and then divided into 75 ml amounts and put in glass bottles.
Experimental Example 1 Measurement of concentration of alcohol in blood in animal
4-week-old male Wistar rats, as an experiment animal, were pre-reared by being allowed to eat solid feed and drink tap water ad libitum for 1 week. After the pre-rearing was finished, the rats were provided with only water and no food for 12 hours before the experiment. The starved rats were orally administered a solution prepared to contain 0.5 g of the glutathione-containing yeast extract powder of Example 1 , 0.2 g of the Rosa roxburghi extract powder of Example 2, 0.1 g of the Engelgarditia chrysolepsis HANCE extract powder of Example 2, 0.1 g of the Nelumbo nucifera extract powder of Example 2 and 0.2 g of water soluble zinc per 7.5 ml of distilled water by using a stomach sonde at a dosage of 7.5 ml/kg. After 30 minutes, ethanol was orally administered to the rats at a dosage of 3 g/kg. As a control group, a solution in which 0.2 g of the Rosa roxburghi extract powder, 0.1 g of the Engelgarditia chrysolepsis HANCE extract powder, and 0.1 g of the Nelumbo nucifera extract powder were dissolved (Comparative Example 1 ), a solution prepared by additionally dissolving 0.2 g of water soluble zinc in the solution of Comparative Example 1 (Comparative Example 2), and a solution prepared by additionally dissolving 0.5 g of the glutathione-containing yeast extract powder in the solution of Comparative Example 1 (Comparative Example 3) were used.
Blood was collected by orbital sinus blood sampling 30 minutes, 1 hour, 3 hours, 6 hours, and 9 hours after administration of ethanol. The collected blood was centrifuged for 10 minutes and serum was separated. The content of ethanol in the serum was analyzed using F-kit ethanol (Roche Diagnostics Corporation).
For reference, the functional principle and kit protocol of the F-kit ethanol are as follows. * Functional Principle
Ethanol is oxidized by the enzyme ADH in the liver to form acetaldehyde. In this process, NADH is produced in the presence of NAD+ as a coenzyme. The amount of NADH is measured at an absorbance of 340 nm.
ethanol + NAI) ' > acetaldehyde + NADH + H'
(AD- ])
* Kit protocol
© Mix 3 ml of a reaction mixture (potassium phosphate buffer (pH 9) + purified NAD+) with 0.1 ml of 10 times diluted blood (D Incubate the mixture at 200C for 3 minutes
© Measure absorbance (A1 ) at 340 nm
© Add 0.05 ml of alcohol dehydrogenase (ADH)
© Incubate the resultant at 200C for 5 minutes
© Measure absorbance (A2) at 340 nm D Concentration = 0.7259/3.6 X ΔA
ΔA = sample (A2-A1 ) - blank test (A2-A1 )
The obtained results are illustrated in FIG. 1. Referring to FIG. 1 , the composition comprising the Rosa roxburghi fruit, the Engelgarditia chrysolepsis HANCE leaf, Nelumbo nucifera (combination of herb extracts), water soluble zinc, and the glutathione-containing yeast extract exhibited an excellent ability at reducing alcohol concentration in blood, compared with the composition of Comparative Example 1 (combination of herb extracts), the composition of Comparative Example 2 (combination of herb extracts + water soluble zinc), and the composition of Comparative Example 3 (combination of herb extracts+ glutathione-containing yeast extract).
Experimental Example 2
Measurement of concentration of acetaldehvde in blood in animal
4-week-old male Wistar rats, as an experiment animal, were pre-reared by being allowed to eat solid feed and drink tap water ad libitum for 1 week. After the pre-rearing was finished, the rats were provided with only water and no food for 12 hours before the experiment. The starved rats were orally administered with a solution prepared to contain 0.5 g of the glutathione-containing yeast extract powder of Example 1 , 0.2 g of the Rosa roxburghi extract powder of Example 2, 0.1 g of the Engelgarditia chrysolepsis HANCE extract powder of Example 2, 0.1 g of the Nelumbo nucifera extract powder of
Example 2 and 0.2 g of water soluble zinc per 7.5 ml of distilled water by using a stomach sonde at a dosage of 7.5 ml/kg. After 30 minutes, ethanol was orally administered to the rats at a dosage of 3 g/kg. As a control group, a solution in which 0.2 g of the Rosa roxburghi extract powder, 0.1 g of the Engelgarditia chrysolepsis HANCE extract powder, and 0.1 g of the Nelumbo nucifera extract powder were dissolved (Comparative Example 1 ), a solution prepared by additionally dissolving 0.2 g of water soluble zinc in the solution of Comparative Example 1 (Comparative Example 2), and a solution prepared by additionally dissolving 0.5 g of the glutathione-containing yeast extract powder in the solution of Comparative Example 1 (Comparative Example 3) were used. Blood was collected by orbital sinus blood sampling 30 minutes, 1 hour, 3 hours, 6 hours, and 9 hours after administration of ethanol. The collected blood was centrifuged at 3000 rpm at 4°C for 15 minutes and serum was separated. The content of acetaldehyde in the serum was analyzed using F-kit acetaldehyde (Roche Diagnostics Corporation). For reference, the functional principle and kit protocol of the F-kit acetaldehyde are as follows.
* Functional Principle
Acetaldehyde is oxidized by the enzyme ALDH in the liver to form acetic acid. In this process, NADH is produced in the presence of NAD+ as a coenzyme. The amount of NADH is measured at an absorbance of 340 nm.
acetaldehyde + tf:,()+ NAf) ' ;• acetic acid v- NAf)H + //'
(A[J M 1)
* Kit protocol (D Mix 3 ml of a reaction mixture (potassium phosphate buffer (pH 9) + purified
NAD+) with 0.2 ml of 10 times diluted blood
(D Incubate the mixture at 200C for 3 minutes
(D Measure absorbance (A1 ) at 340 nm
® Add 0.05 ml of acetaldehyde dehydrogenase (ALDH) © Incubate the resultant at 200C for 5 minutes
© Measure absorbance (A2) at 340 nm
D Concentration = 0.7158/3.6 X ΔA
ΔA = sample (A2-A1 ) - blank test (A2-A1 )
The obtained results are illustrated in FIG. 2. Referring to FIG. 2, the composition of Example 1 comprising the Rosa roxburghi fruit, the Engelgarditia chrysolepsis HANCE leaf, Nelumbo nucifera (combination of herb extracts), water soluble zinc, and the glutathione-containing yeast extract exhibited an excellent ability at reducing acetaldehyde concentration in blood, compared with the composition of Comparative Example 1 (combination of herb extracts), the composition of Comparative Example 2 (combination of herb extracts + water soluble zinc), and the composition of Comparative Example 3 (combination of herb extracts+ glutathione-containing yeast extract).
Experimental Example 3 Measurement of concentration of alcohol in blood in the human body
Experiments were performed using the composition of Example 3 comprising the raw materials prepared in Examples 1 and 2 in a crossover experiment, a double blind method, and a randomization test. The subjects were allowed to have a washout period between a control group and a test group. All the subjects were called 1 hour before the experiment and provided with the same meal. 2 hours after the meal, the composition of Example 3 was orally administered to the subjects. From 30 minutes after oral administration of the composition of Example 3, the subjects drank 100 g of alcohol for a 1 hour duration. The subjects were provided with 30 g of shrimp chips, as a side dish, called "saewoggang" in Korean and ate them while drinking the alcohol. The subjects were provided with no food for 4 hours after alcohol drinking. 3 ml of blood samples were taken at 0 hour, 1 hour, 2 hours, 5 hours, and 8 hours after alcohol drinking, and then the taken blood was put right away in a vacuum gel tube. Then, the blood was centrifuged at 3000 rpm at 40C for 15 minutes and serum was separated out.
The content of ethanol in the serum was analyzed using F-kit ethanol (Roche Diagnostics Corporation).
The obtained results are illustrated in FIG. 3. Referring to FIG. 3, the composition of Example 3 of the present invention exhibited an excellent ability at reducing alcohol concentration in blood, compared with the control groups.
Experimental Example 4
Measurement of concentration of acetaldehvde in blood in the human body
Experiments were performed using the composition of Example 3 comprising the raw materials prepared in Examples 1 and 2 in a crossover experiment, a double blind method, and a randomization test. The subjects were allowed to have a washout period between a control group and a test group. All the subjects were called 1 hour before the experiment and provided with the same meal. 2 hours after the meal, the composition of Example 3 was orally administered to the subjects. From 30 minutes after oral administration of the composition of Example 3, the subjects drank 100 g of alcohol for a 1 hour duration. The subjects were provided with 30 g of shrimp chips, as a side dish, called "saewoggang" in Korean and ate them while drinking the alcohol. The subjects were provided with no food for 4 hours after alcohol drinking. 3 ml of blood samples taken at 0 hour, 1 hour, 2 hours, 5 hours, and 8 hours after alcohol drinking were collected, and then the collected blood was put right away in a vacuum gel tube. Then, the blood was centrifuged at 3000 rpm at 4°C for 15 minutes and serum was separated. The content of acetaldehyde in the serum was analyzed using F-kit acetaldehyde (Roche Diagnostics Corporation).
The obtained results are illustrated in FIG. 4. Referring to FIG. 4, the composition of Example 3 of the present invention exhibited an excellent ability at reducing acetaldehyde concentration in blood, compared with the control groups.

Claims

1. A composition for preventing or treating a hangover, comprising a glutathione-containing yeast extract, water soluble zinc, a Rosa roxburghi extract, an Engelgarditia chrysolpsis HANCE extract, and a Nelumbo nucifera extract.
2. The composition of claim 1 , comprising 25-45 parts by weight of water soluble zinc, 25-45 parts by weight of the Rosa roxburghi extract, 10-25 parts by weight of the Engelgarditia chrysolpsis HANCE extract and 10-25 parts by weight of the Nelumbo nucifera extract with respect to 100 parts by weight of the glutathione-containing yeast extract.
3. The composition of claim 1 , further comprising vitamin B, vitamin C, vitamin E, β-carotin, Ca, Mg, Zn, Lecithin, alanine, taurine, maltol, fructose, oligosaccharide, Ganoderma lucidum, or mixtures thereof.
4. The composition of any one of claims 1 through 3, in the form of a solution, a suspension, a powder, a granule, a tablet, a capsule, a pill or an extract.
5. The composition of claim 1 or 3, in the form of a tea, a jelly, or a beverage.
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CN104432382A (en) * 2014-12-18 2015-03-25 杜超峰 Chinese wine-flavour roxburgh rose sour soup composition and preparation method
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KR101498780B1 (en) 2013-04-18 2015-03-04 씨제이제일제당 (주) Composition for preventing or treating hangover
KR20170046540A (en) * 2015-10-21 2017-05-02 주식회사 피코엔텍 Composition for preventing and relieving Hangover
KR101925096B1 (en) 2018-05-02 2018-12-04 아미코젠주식회사 A manufacturing method of hangover-eliminating enzyme powder and a composition for relieving hangover comprising thereof
KR102614229B1 (en) * 2020-06-30 2023-12-19 (주)에스티알바이오텍 A composition for relief of alcoholic hangover comprising the fermentative products of rice bran as an active ingredient
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