KR19980018190A - Herbicide composition and weed control method containing phenoxycarboxylic acid derivatives and microorganisms of the genus Epicoccus - Google Patents

Abstract

An herbicide composition comprising Epicoccus genus, which contains weed-controlling microorganisms and phenoxycarboxylic acid derivatives as active ingredients.

Provided are novel herbicide compositions. This herbicide composition can effectively control all weeds of the wild family, such as wild flowers, tadpoles and beetles. In particular, it is very useful as agricultural materials because it is possible to efficiently control strong weeds, including all-seasoned eggs, in a short time in a low dose.

Description

Herbicide composition and weed control method containing phenoxycarboxylic acid derivatives and microorganisms of the genus Epicoccus

TECHNICAL FIELD The present invention relates to a technique for efficiently and in a short time controlling a strong weed resulting in a reduced yield of cultivated crops.

Phenoxy based herbicides are herbicides containing phenoxycarboxylic acid derivatives such as sodium 2,4-dichlorophenoxy acetate as an active ingredient. This herbicide is a selective herbicide with a hormonal action, and has a wide range of excellent herbicidal effects on perennial weeds such as annual broadleaf weeds and non-nude beetles, tadpoles, scabbards, and hair stalks, but not sufficient against tallow. Can't get it.

On the other hand, due to the problem of environmental pollution by mass use of chemical pesticides, the development of weed control agents and their methods that do not rely on chemical pesticides is desired. As a control method of all five insects which do not use a chemical pesticide, the method of Unexamined-Japanese-Patent No. 5-59084 is known, for example. This method inoculates the spores (conidia) of one strain of a strain of Hyphomycetes isolated from the leprosy stems of the water seals, and inoculates the spores to develop the spores. It is a control method by suppressing fertility. In addition, one strain of the genus Hypomitestes is the same species as the Epicoccus and nematosporus of the present invention (Agriculture and Horticulture, Vol. 63, No. 6, No. 6 (1988), page 63). .

However, these control methods are generally used alone of the microorganisms belonging to Epicoccus, nematosporus, it takes a long time to show the control effect on all five, and the control effect was not satisfactory.

The present invention has been made to solve the problems related to weed control as described above, and its object is to provide a more effective weed control means.

MEANS TO SOLVE THE PROBLEM As a result of earnestly examining in order to solve such a problem, it discovered that the control effect on weeds improves synergistically by using together the microbe and phenoxycarboxylic acid derivative which belong to an Epicock chorus nematosporus in a weed. The present invention was completed.

That is, the present invention relates to a herbicidal composition, which belongs to the genus Epicoccus, and contains microorganisms having weed control and phenoxycarboxylic acid derivatives as active ingredients.

Moreover, this invention relates to the weed control method characterized by spraying on the weed to the herbicide composition of the said description.

In another aspect, the present invention is to the weed control method, characterized in that spraying on the weeds herbicide belonging to the genus Epicockorus and containing weed control ability as an active ingredient and a herbicide containing phenoxycarboxylic acid derivative as an active ingredient. It is about.

Chemicals that are the main component of herbicides generally have a bad effect on the growth of microorganisms. For example, 3-isopropyl-2,1,3-benzo-thiadiazinone- ( 4) 2,2-dioxide, such as bendazoles, when mixed with the microorganisms belonging to the epicoccus genus, completely kills the microorganisms belonging to the epicoccus genus.

Therefore, it is surprising that the phenoxycarboxylic acid derivative and the microorganism belonging to the epicoccus genus synergistically improve the control effect on the weeds.

Hereinafter, the present invention will be described in detail.

First, the herbicide composition of the present invention will be described.

Epicoccus genus in the present invention is listed on the 27th (July 15, 1988) notice summary of the Japanese Society of Plant Pathology Conference, and 63, No. 63 (1988), 『Agriculture and Horticulture』. Epicoccosorus nematosporus gen. et sp. nov. Means a genus that includes a.

The microorganism used in the present invention is not particularly limited as long as it belongs to the genus Epicoccusus and has a weed control concentration, but is preferably Epicoccusus nematosporus. E. coli, nematosporus JTK-003 strain, epicock cosphorus nematosporus JTK-028 strain, epicock cousrus nematosporus JTK-029 strain, epicock cousus nematosporus JTK-030 strain, and Epicock cholesterus, nematosporus K-035 strain, etc. are mentioned.

These strains are deposited in the following numbers, respectively, at the Institute of Biotechnology Industrial Technology Research Institute (Ibarakiken, Tsukubashi Higashi, Japan, 1, 3, 3 Goo). The Accession No. of the Epicoccus and Nematosporus JTK-003 strain is FERM BP-4906 (December 5, 1994), and the Epicocorus and Nematosporus JTK-028 strain is FERM BP-4907 ( December 5, 1994), Epicock cousus nematosporus JTK-029 strain is FERM BP-4908 (December 5, 1994), Epicock cousrus nematosporus JTK- 030 strains are FERM BP-4909 (December 5, 1994) and Epicock cousrus nematosporus K-035 strains are FERM BP-4477 (Dec. 25, 1993).

These strains are isolated from leprosy plants, and their microbial properties are as follows.

It is an aerobic bacterium, and on a potato sudangcheon basin, it forms spherical conglomerates of flesh in the center of colony and forms dark brown structure in the shape of the periphery. Later on, a dark brown conidia forms on the dark brown structure. Sometimes white aerial hyphae are visible. Conidia (conidia spores) are nematodes or sinwolhyeong in the curved shape of monospores and are colorless. The size of conidia is about 23 to 80 x 3 to 5 mu m.

The results were described in Epicoccosorus nematosporus gen., 63, No. 63, No. 6 (1988), page 63, and the Japanese Society of Plant Pathology Lecture Abstracts (August 15, 1988). et sp. As a result of comparative examination with the bacteriological properties of nov., the strains were identified as Epicoccus and nematosporus because the colony and the shape of the conidia were the same.

It is not necessary to use a special method for culturing the strains described above, and the same method as the known strains belonging to the genus Epicoccus can be used. As the medium, any medium of synthetic medium or natural medium can be used as long as the medium contains a magnetizable carbon source, nitrogen source, inorganic matter and necessary growth promoting substances. Examples of specific badges include oatmeal sudang agar badges, oatmeal agar badges, potato sudang agar badges, V-8 juice agar badges, and zapax agar agar badges. In culture, the temperature is maintained at 15 to 33 ° C, preferably 23 to 25 ° C, and PH at 3 to 10, preferably 5 to 7 ° C. When cultured for 3 to 14 days under the above conditions, a sufficient amount of conidia are formed on the surface of the medium.

When the strain is used in the herbicide composition, microbial cells can be used, but preferably the conidia produced by the strain are used. The conidia can be recovered by pouring sterile water onto the flora and scraping the surface of the media with a brush. By this method, 1 × 10 ⁷ -10 ⁹ conidia can be obtained per one Petri dish having a diameter of 9 cm.

In the present invention, the microorganism and the phenoxycarboxylic acid derivative are used as active ingredients.

Herein, the phenoxycarboxylic acid derivative refers to a compound in which phenoxycarboxylic acid is a basic skeleton and a part of hydrogen of a benzene ring or hydrogen of a carboxyl group is substituted.

The phenoxycarboxylic acid derivative used in the present invention may be any compound as long as it satisfies the above definition, but it is preferable to use a compound represented by the following general formula (I) or a hydrate thereof.

Wherein, X represents a C1 or CH _3, n is an integer of 1 to 5. In addition, R represents an alkali metal, dialkyl ammonium, or an alkyl group. Examples of the alkali metal include sodium and potassium. Examples of the dialkyl ammonium include dimethyl ammonium, and the alkyl group includes methyl, ethyl, propyl and butyl.

More preferable phenoxycarboxylic acid derivatives include sodium 2,4-dichlorophenoxy acetate, 2,4-dichlorophenoxy acetate dimethylamine, ethyl 2,4-dichlorophenoxy acetate and 2-methyl-4-chlorophenoxy acetate Monohydrate of sodium, ethyl 2-methyl-4-chlorophenoxy acetate, butyl 2-methyl-4-chlorophenoxy acetate, sodium 2-methyl-4-chlorophenoxy butyrate, 2-methyl-4-chlorophenoxy Ethyl butyrate or α- (2-methyl-4-chlorophenoxy) potassium propionate.

When the herbicide composition of the present invention is used as a herbicide, a condenser and a phenoxycarboxylic acid derivative of a microorganism are usually suspended and used in water. The conidia party content of 10 ^{2-10 8} / ㎖, to preferably appropriate to 10 ^{4-10 7} / ㎖. The concentration of the phenoxycarboxylic acid derivative varies depending on the kind of the substance used, but it is appropriate to set it to 0.0001 to 20% by weight, preferably 0.001 to 10% by weight. Examples of the plant to which the herbicide composition is to be controlled in the present invention include tadpoles such as wild grasses, and tadpoles such as cooked bones and gwangneunggol, algae dongsa, shackles, beetle dongs and tadpoles, among which are examples. For example, if the target is the target of the control, the lush period is preferable from the germination, and the medium growth period (生 바람직 中期) is particularly preferable immediately after germination. The spray amount in the nonon of the herbicide composition of the present invention is not particularly limited as long as it can effectively control the weeds, but the conidia are 10 ⁸ to 10 ^{11 per} 10 ars, and the phenoxycarboxylic acid derivative is 5 to 10 g. It is preferable to spray to a degree.

According to the present invention, the herbicide composition belonging to the genus Epicockorus and containing weed control ability and the phenoxycarboxylic acid derivative as an active ingredient may be used as it is for the plant to be treated, but is generally an inert liquid carrier. Or it is mixed with a solid carrier and added with other adjuvant as needed, and it is prepared by using it in the form of a conventionally used form, for example, powder, granule, hydrating agent, emulsion, fluid formulation, etc. As the carrier, any one of a solid or a liquid can be used as long as it is usually used in agricultural horticultural agents, and the carrier is not limited to a specific one. For example, examples of the solid carrier include mineral powders such as clay, talc and bentonite, plant component powders such as soybean powder and starch, and high molecular compounds such as polyvinyl alcohol and polyamethylene glycol. Examples of the liquid carrier include various oils, various organic solvents, water and the like. As an adjuvant, surfactant, binder, stabilizer, etc. which are normally used for agricultural horticultural agents can be used individually or in combination as needed. As the surfactant, nonionic, anionic, cationic and cationic ones can be appropriately used.

Examples of the adjuvant include lignin sulfonate, alginic acid, polyvinyl alcohol, gum arabic, carboxymethyl cellulose (CMC), acid isopropyl phosphate (PAP), and the like, and can be used alone or in combination.

Hereinafter, the weed control method of the present invention will be described.

There are two methods for the weed control method of the present invention. One is the method of formulating said herbicide composition and spraying on weeds. The other is a method of spraying weeds with both herbicides containing microorganisms belonging to the genus Epicoccusus as an active ingredient and herbicides containing a phenoxycarboxylic acid derivative as an active ingredient. In the latter method, the spraying order of the two kinds of herbicides is not particularly limited but is preferably sprayed at the same time.

EXAMPLE

Example 1

Epicoccus and Nematosporus K-035 strains were transplanted onto PDA medium, and left to stand for about 7 days under bright conditions at 25 ° C. A large amount of spores was formed on the surface of the medium. Sterile water was poured onto the medium, and spores were collected by scraping the surface of the medium with a brush. Spores were recovered from about 1 × 10 ^{8 to} 1 × 10 ⁹ .

Example 2

The one-month-old cultivated oar was grown in the pot and used for the test of control effect. Conidia and 2,4-D soda salt (Ishiharase, 2,4-dichlorophenoxy acetate monohydrate 95%) of the strain of Epicoccus and Nematosporus K-035 recovered by the above method in 100 ml of water. Containing) or MCP soda salt (containing Ishihara, 19.5% of 2-methyl-4-chlorophenoxy acetate) was suspended in predetermined amounts, respectively.

This suspension was sprayed using a nebulizer until the stems and leaves of all five were evenly wetted. Afterwards, the alien was placed in a humid room maintained at 25 ° C. and 100% humidity for 24 hours.

After that, the herbicidal activity was determined by standing in a greenhouse at 25 ° C. for 2 weeks. The herbicidal activity was determined by examining the degree of mortality of all five lobes and calculating the average value (3 repetitions). The degree of mortality was assessed by the lunar tube (관) with a healthy state of 0 and total stem and leaf deaths of 100.

The results are shown in Table 1.

As described above, when the microorganisms belonging to the genus Epicoccusus and phenoxycarboxylic acid derivatives were mixed and used, remarkably high herbicidal effects were obtained compared to the case where they were used alone.

Example 3

The herbicidal activity was determined in the same manner as in Example 2 using the Epicoccusrus nematosporus JTK-030 strain instead of the Epicoccusrus nematosporus K-035 strain. The results are shown in Table 2.

As in Example 2, when a mixture of microorganisms belonging to the genus Epicoccusus and phenoxycarboxylic acid derivatives were used, a much higher herbicidal effect was obtained than when used alone.

Preparation Example 1 (Liquid Preparation)

1 l of water suspended 10 ⁹ conidia of epicoccus and nematosporus K-035 strains and 2 g of 2,4-D soda salt (containing 95% of 2,4-dichlorophenoxy acetate monohydrate). Was prepared.

Preparation Example 2 (Hydrating Agent)

10 ⁹ conidia of the mixed solution of 1 ml Epicoccus and Nematosporus K-035 strains containing 9% maltose, 1% clay and 90% water were suspended. After drying, the dried product was mixed with 2 g of 2,4-D soda salt (containing 95% of 2,4-dichlorophenoxy acetate monohydrate) and ground to prepare a hydrating agent.

Preparation Example 3 (Hydrating Agent)

10 ⁹ conidia of epicoccus and nematosporus K-035 strains were suspended per 1 ml of a mixture of 10% skim milk and 90% water. After drying, 2 g of 2,4-D soda salt (containing 95% of 2,4-dichlorophenoxy acetate monohydrate) was mixed and ground to prepare a hydrating agent.

By using a combination of microorganisms belonging to the epicoccusus and weed control ability with phenoxycarboxylic acid derivatives, all weeds of the genus such as wild rapeseed, tadpole, and beetle can be effectively controlled. In particular, it is possible to efficiently control the weeds, including the long-haul with low doses in a short period of time and is very useful as agricultural materials.

Claims (7)

An herbicide composition comprising Epicoccus genus, which contains weed-controlling microorganisms and phenoxycarboxylic acid derivatives as active ingredients. The herbicide composition according to claim 1, wherein the microorganism belonging to the genus Epicoccusus and having weed control ability is a microorganism belonging to the Epicoccusrus nematosporus. The microorganisms according to claim 2, wherein the microorganisms belonging to the Epicoccus genus nematosporus are the Epicoccus genus nematosporus JTK-003 strain, the Epicoccus genus nematosporus JTK-028 strain, and the Epicocosaurus genus A herbicide composition characterized in that it is at least one strain selected from the group consisting of a Matosporus JTK-029 strain, an Epicock cousus nematosporus JTK-030 strain, and an Epicock cousrus nematosporus K-035 strain. The herbicidal composition according to any one of claims 1 to 3, wherein the phenoxy carboxylic acid derivative is a compound represented by the following general formula (I) or a hydrate thereof. (Wherein X represents C1 or CH ₃ , R represents an alkali metal, a dialkylammonium, or an alkyl group, and n represents an integer of 1 to 5). The phenoxycarboxylic acid derivative according to any one of claims 1 to 3, wherein the phenoxycarboxylic acid derivative is monohydrate of 2,4-dichlorophenoxy acetate, 2,4-dichlorophenoxy acetate dimethylamine, 2,4-dichloro Ethyl phenoxy acetate, sodium 2-methyl-4-chlorophenoxy acetate, ethyl 2-methyl-4-chlorophenoxy acetate, butyl 2-methyl-4-chlorophenoxy acetate, 2-methyl-4-chlorophenoxy A herbicide composition characterized in that it is sodium butyrate, ethyl 2-methyl-4-chlorophenoxy or potassium α- (2-methyl-4-chlorophenoxy) propionate The method of preventing weeds, wherein the herbicide composition according to any one of claims 1 to 5 is sprayed on the weeds. A weed control method comprising spraying on a weed, a herbicide belonging to the genus Epicoccus, and containing a microorganism having weed control as an active ingredient, and a herbicide containing a phenoxycarboxylic acid derivative as an active ingredient.