JPH1059813A - Herbicidal composition comprising phenoxycarboxylic acid derivative and microorganism of genus epicoccosorus and controlling of weed - Google Patents

Abstract

PROBLEM TO BE SOLVED: To enable the obtaining of a herbicidal composition capable of efficiently controlling strongly noxious weeds with a low dose in a short period by including a microorganism of the genus Epicoccosorus (including Eoicoccosorus nematosporus gen. et sp. nov.) and a phenoxycarboxylic acid derivative therein. SOLUTION: This herbicidal composition comprises a microorganism belonging to Epicoccosorus nematosporus and a phenoxycarboxylic acid derivative represented by the formula [X is Cl or CH3 ; R is an alkali metal, a dialkylammonium or an alkyl; (n) is 1-5] as active ingredients. An Epicoccosorus nematosporus JTK-003 strain, etc., are cited as the microorganism and sodium 2,4-dichlorophenoxyacetate monohydrate, etc., are cited as the compound represented by the formula. The herbicidal composition is capable of controlling weeds of the family Cyperaceae such as barnyard grass, bulrushes or water nutgrass and is especially effective against strongly noxious weeds including water chestnut and useful as an agricultural material.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a technique for controlling a highly harmful weed which causes a decrease in the yield of a cultivated crop with a low dose and efficiently and in a short time.

[0002]

2. Description of the Related Art A phenoxy acid herbicide is a herbicide containing a phenoxycarboxylic acid derivative such as sodium 2,4-dichlorophenoxyacetate as an active ingredient. This herbicide is a selective herbicide having a hormonal action, and has a wide and excellent herbicidal effect on perennial weeds such as annual broadleaf weeds other than gramineae and urikawa, sedge flies, fireflies, edaka, and pine pine, The effect is not enough for Krogwai.

On the other hand, in recent years, there has been a demand for the development of a weed controlling agent which does not rely on chemical pesticides and a method of using the same, because of the problem of environmental pollution due to heavy use of chemical pesticides. As a method for controlling black clover without using chemical pesticides, for example, Japanese Patent Publication No. 5-59
No. 084 is known. This method
A control method by inoculating spores (conidia) of a strain of a new genus of the class of Hyhomycetes isolated from diseased stems of Krogwai into Krogwai, causing the Krogwai to become diseased, thereby suppressing the growth and fertility of the Krogwai. is there. The strain of a new genus of the class Hyhomomycetes is the same genus as Epicoccosulus nematosporus of the present invention (Agriculture and Horticulture, Vol. 63, No. 6, No. 6, 1988).
3). However, these control methods generally use a microorganism belonging to Epicoccosolus nematosporus alone, and it takes a long time for the control effect against kuroguwai to be expressed, and the control effect is not satisfactory.

[0004]

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems relating to weed control, and an object of the present invention is to provide a more effective means for controlling weeds. It is in.

[0005]

Means for Solving the Problems As a result of intensive studies to solve the above problems, the present inventor has found that a microorganism belonging to Epicoccosus nematosporus and a phenoxycarboxylic acid derivative are used in combination and applied to weeds. We found that the control effect on weeds improved synergistically,
The present invention has been completed. That is, the present invention is a herbicidal composition comprising, as active ingredients, a microorganism belonging to the genus Epicoccosus and having the ability to control weeds, and a phenoxycarboxylic acid derivative.

[0006] The present invention also provides a method for controlling weeds, which comprises spraying the herbicidal composition described above on weeds. Furthermore, the present invention provides a weed which is characterized by spraying a herbicide belonging to the genus Epicoccoculus, which contains a microorganism having an ability to control weeds, as an active ingredient and a herbicide containing a phenoxycarboxylic acid derivative as an active ingredient. It is a control method.

[0007] The chemical substance which is the main component of the herbicide generally has a bad influence on the growth of microorganisms. For example, a herbicide belonging to diazine herbicide,
Isopropyl-2,1,3-benzo-thiadiazinone-
(4) When mixed with a microorganism belonging to the genus Epicoccosus, a bentazone agent or the like containing -2,2-dioxide as a main component completely kills a microorganism belonging to the genus Epicoccosus. Therefore, it is an extremely surprising result that the control effect on weeds is synergistically improved by mixing a phenoxycarboxylic acid derivative with a microorganism belonging to the genus Epicoccosus.

Hereinafter, the present invention will be described in detail. First, the herbicidal composition of the present invention will be described. The term "Epicococsolus" in the present invention refers to the 27th abstract of the meeting of the Japanese Society for Plant Pathology (published on March 15, 1988) and "Agriculture and Horticulture" Vol. 63, No. 6, (1988), page 63. Epicoccosorus nematosporus gen. Et s described
It means "genus" including p.nov.

The microorganism used in the present invention is not particularly limited as long as it is a microorganism belonging to the genus Epicoccosolus and has the ability to control weeds, but is preferably a microorganism belonging to Epicoccosus nematosporus and having the ability to control weeds.
Among these, preferred strains include Epicoccoclas nematosporus JTK-003, Epicoccolas nematosporus JTK-028, Epicoccolas nematosporus JTK-029, Epicoccolas nematosporus JTK-030, and Epicoccolas Koma-35 and others. it can.

These strains have been deposited with the Research Institute of Biotechnology and Industrial Technology of the Ministry of International Trade and Industry (1-1-3 Higashi, Tsukuba, Ibaraki, Japan) under the following numbers. Epicoccosolus nematosporus JTK-0
The deposit number of the 03 strains is FERM BP-4906 (Original deposit date January 1994
February 5), Epicococsolus nematosporus JTK
-028 strain is FERM BP-4907 (Original deposit date December 5, 1994)
Sun), Epicococsolus nematosporus JTK-02
Nine strains are FERM BP-4908 (originally deposited on December 5, 1994), and Epicoccosolus nematosporus JTK-030 strain is FE
RM BP-4909 (Deposited on December 5, 1994), Epicoccoculus nematosporus K-035 strain was FERM BP-4477
(Original deposit date: November 25, 1993).

These strains have been isolated from diseased plants and have the following bacteriological properties. It is an aerobic bacterium, and on a potato sucrose agar medium, forms a spherical, flesh-colored conidium mass at the center of the colony and forms a spherical, black-brown structure at the periphery. Later, a flesh-colored conidium mass is formed on the peripheral black-brown structure. Also, white aerial hyphae may be seen. Conidia (conidia) are nematodes or crescents from the curved shape of a single cell, and are colorless. The size of conidia is around 23-80 × 3-5 μm.

The above results are described in " Epicoccosorus " described in "Agriculture and Horticulture", Vol. 63, No. 6, (1988), p. 63, and Preprints of the Abstracts of the Annual Meeting of the Japanese Society of Plant Pathology, p. 27, published on March 15, 1988. nov ., the colony shape and conidia morphology are the same.
It was identified as nematosporus.

For culturing the above-mentioned strains, it is not necessary to use a special method, and the same method as that used for known strains belonging to the genus Epicoccosus can be used. As the medium, any synthetic medium or natural medium can be used as long as the medium appropriately contains assimilable carbon sources, nitrogen sources, inorganic substances, and necessary growth promoting substances. Specific examples of the medium include an oatmeal sucrose agar medium, an oatmeal agar medium, a potato sucrose agar medium, a V-8 juice agar medium, a Tupec-Docs agar medium, and the like. During culturing, the temperature is preferably 15 to 33 ° C, preferably
It is desirable to maintain the pH at 23-25 ° C and the pH at 3-10, preferably 5-7. When culture is performed for about 3 to 14 days under the above conditions, a sufficient amount of conidia is formed on the surface of the medium.

When this strain is used in a herbicidal composition,
Microbial cells can be used, but preferably, conidia produced by the present strain are used. Conidia can be collected by pouring sterile water onto the microbiota and scraping the surface of the medium with a brush. By this method, 1 × 10 ⁷ to 10 ⁹ conidia can be obtained per petri dish having a diameter of 9 cm. The present invention comprises the above microorganism and a phenoxycarboxylic acid derivative as active ingredients. Here, the phenoxycarboxylic acid derivative refers to a compound having phenoxycarboxylic acid as a basic skeleton and in which a part of hydrogen of a benzene ring or a hydrogen of a carboxyl group is substituted. The phenoxycarboxylic acid derivative used in the present invention may be any one as long as it satisfies the above definition, but it is preferable to use a compound represented by the following general formula (I) or a hydrate thereof.

[0015]

Embedded image

Here, X represents Cl or CH ₃ , and n represents an integer of 1 to 5. R represents an alkali metal, dialkylammonium, or alkyl group. Examples of the alkali metal include sodium and potassium, and examples of the dialkylammonium include dimethylammonium.Examples of the alkyl group include a methyl group, an ethyl group, a propyl group, and a butyl group. it can.

More preferred phenoxycarboxylic acid derivatives include sodium 2,4-dichlorophenoxyacetate, dimethylamine 2,4-dichlorophenoxyacetate,
Ethyl 2,4-dichlorophenoxyacetate, 2-methyl-
Sodium 4-chlorophenoxyacetate monohydrate, 2-
Ethyl methyl-4-chlorophenoxyacetate, butyl 2-methyl-4-chlorophenoxyacetate, 2-methyl-4-
Sodium chlorphenoxybutyrate, ethyl 2-methyl-4-chlorophenoxybutyrate, or α- (2-methyl-4
-Chlorphenoxy) potassium propionate and the like.

When the herbicidal composition of the present invention is used as a herbicide, a conidia of a microorganism and a phenoxycarboxylic acid derivative are usually used by suspending them in water. At this time, the concentration of conidia is 10 ² to 10 ⁸ / ml, preferably 10 ⁴ to 10 ⁴ / ml.
It is appropriate to use 10 ⁷ cells / ml. The concentration of the phenoxycarboxylic acid derivative varies depending on the type of the substance used, but is 0.0001 to 20% by weight, preferably 0.0001 to 20% by weight.
Suitably, it is 1 to 10% by weight. Examples of plants to be controlled by the herbicidal composition of the present invention include grasses such as Nobies and fireflies such as canine fireflies and fireflies; In particular, it is effective for Krogwai. When the herbicidal composition of the present invention is to be applied, for example, in the case where Kuroguwai is to be controlled, the prosperity period is preferable before germination, and the midlife period is particularly preferable immediately after germination of Kuroguwai. The application amount of the herbicidal composition of the present invention in the paddy field is not particularly limited as long as the weeds can be effectively controlled, but the conidium is 10 ⁸ to 10 ¹¹ per 10 ares, and the phenoxycarboxylic acid derivative is 5 to 10 kg. It is preferable to spray to a degree.

The herbicidal composition comprising a microorganism belonging to the genus Epicoccosolus and capable of controlling weeds according to the present invention and a phenoxycarboxylic acid derivative as active ingredients may be used as it is for the plant to be treated. Is mixed with an inert liquid carrier or solid carrier, and if necessary, other auxiliaries are added to prepare a commonly used formulation such as powder, granule, wettable powder, emulsion, and flowable formulation. used. As the carrier, any solid or liquid carrier can be used as long as it is generally used for agricultural and horticultural medicines, and is not limited to a specific carrier. For example,
Examples of the solid carrier include mineral powders such as clay, talc and bentonite, vegetable powders such as soybean powder and starch, and high molecular compounds such as polyvinyl alcohol and polyalkylene glycol. Examples of the liquid carrier include various oils, various organic solvents, water and the like. As the auxiliary agent, a surfactant, a binder, a stabilizer and the like, which are usually used for agricultural and horticultural medicines, can be used alone or in combination as appropriate, if necessary. As the surfactant, a nonionic, anionic, cationic, or amphoteric surfactant can be used as appropriate.

As an auxiliary, lignin sulfonate,
Alginic acid, polyvinyl alcohol, arabic gum, carboxymethyl cellulose (CMC), isopropyl acid phosphate (PAP) and the like can be mentioned.
Or they can be used in combination. Next, the method for controlling weeds of the present invention will be described. In the method for controlling weeds of the present invention,
There are two ways. One is a method of formulating the above herbicide composition and spraying it on weeds. The other is a method of spraying both separately prepared herbicides containing a microorganism belonging to the genus Epicoccosus as an active ingredient and a herbicide containing a phenoxycarboxylic acid derivative as an active ingredient on weeds. In the latter method, the order of application of the two herbicides is not particularly limited, but it is preferable to apply them simultaneously.

[0021]

BEST MODE FOR CARRYING OUT THE INVENTION

[0022]

【Example】

[Example 1] Epicoccosolus nematosporus strain K-035 was transplanted onto a PDA medium and allowed to stand at 25 ° C under light conditions for about 7 days. As a result, a large amount of spores were formed on the surface of the medium. Sterile water was poured onto the medium, and the spores were collected by scraping the surface of the medium with a brush. 1 × 10 ⁸ spores
１1 × 10 ⁹ pieces could be recovered.

[Example 2] Growing in a pot was carried out, and after 1 month, it was used for a control effect test. Water 1
In 100 ml, the conidia of E. coli Nematosporus K-035 recovered by the above method, and 2,4-
D soda salt (manufactured by Ishihara, containing 95% sodium 2,4-dichlorophenoxyacetate monohydrate) or MCP soda salt (manufactured by Ishihara, containing 19.5% sodium 2-methyl-4-chlorophenoxyacetate) Each was suspended in a predetermined amount. This suspension was sprayed using a sprayer until the foliage of Kuroguwai was evenly and uniformly wetted. After application,
Keep the croquis at 25 ° C and 100% humidity in a moist chamber.
Left for 4 hours. Then, it was further placed in a greenhouse at 25 ° C. for 2 weeks, and herbicidal activity was determined. The herbicidal activity was determined by investigating the degree of dying of Kuroguwai and calculating the average value (3 repetitions). The degree of withering was evaluated as holistic, with 0 being healthy and 100 being completely withered. Table 1 shows the results.

[0024]

[Table 1]

As described above, when a microorganism belonging to the genus Epicoccosus and the phenoxycarboxylic acid derivative were applied in a mixed manner, a much higher herbicidal effect was obtained than when each was applied alone.

Example 3 Herbicidal activity was determined in the same manner as in Example 2 except that E. coli nematospora strain JTK-030 was used instead of E. coli nematospora strain K-035. Table 2 shows the results.

[0027]

[Table 2]

As in Example 2, when a microorganism belonging to the genus Epicoccosus and a phenoxycarboxylic acid derivative are mixed and applied, compared to the case where each is applied alone,
A much higher herbicidal effect was obtained. [Formulation Example 1] (Solution) Epicoccosolus nematosporus K-03 in 1 L of water
5 conidia 10 ⁹ strains and 2,4-D sodium salt (2,4-chlorophenoxy sodium acetate monohydrate 95% content) was 2g suspended, was prepared liquid.

Formulation Example 2 (Wettable powder) Epicoccoculus nematosporus K- per 1 ml of a mixed solution consisting of 9% maltose, 1% clay and 90% water
10 ⁹ conidia of 035 strain were suspended. After air-drying, the dried product was mixed with 2 g of 2,4-D soda salt (containing 95% sodium 2,4-dichlorophenoxyacetate monohydrate) and pulverized to prepare a wettable powder.

[Preparation Example 3] (Wettable powder) 10 ⁹ conidia of Epicoccosulus nematosporus K-035 were suspended per 1 ml of a mixed solution composed of skim milk 10% and water 90%. After air-drying, the dried product was mixed with 2 g of 2,4-D soda salt (containing 95% sodium 2,4-dichlorophenoxyacetate monohydrate) and pulverized to prepare a wettable powder.

[0031]

Industrial Applicability By using a microorganism belonging to the genus Epicoccosus and having the ability to control weeds in combination with a phenoxycarboxylic acid derivative, weeds of the family Cyperaceae, such as flies, fireflies, and sedges, can be effectively controlled. In particular, it can effectively control highly harmful weeds such as kuroguwai in a short period of time and with a low dose, and is extremely useful as agricultural material.

Claims (7)

[Claims] 1. A herbicidal composition comprising, as active ingredients, a microorganism belonging to the genus Epicoccoculus and having the ability to control weeds, and a phenoxycarboxylic acid derivative. 2. The herbicidal composition according to claim 1, wherein the microorganism belonging to the genus Epicoccosus and having the ability to control weeds is a microorganism belonging to Epicoccosora nematosporus. 3. The microorganism belonging to Epicoccosus nematosporus, wherein the microorganism belonging to Epicoccosus nematosporus JT is used.
K-003 strain, Epicococsolus nematosporus JT
K-028 strain, Epicococsolus nematosporus JT
K-029 strain, Epicococsolus nematosporus JT
At least one selected from the group consisting of strain K-030 and strain Epicoccosolus nematosporus K-035;
The herbicidal composition according to claim 2, which is a bacterial strain. 4. A phenoxycarboxylic acid derivative represented by the following general formula (I): (Wherein, X represents Cl or CH ₃ , R represents an alkali metal, dialkylammonium, or alkyl group,
n represents an integer of 1 to 5. The herbicidal composition according to any one of claims 1 to 3, which is a compound represented by the formula: or a hydrate thereof. 5. The method according to claim 1, wherein the phenoxycarboxylic acid derivative is 2,4.
Monohydrate of sodium dichlorophenoxyacetate, 2,
Dimethylamine 4-dichlorophenoxyacetate, 2,4-
Ethyl dichlorophenoxyacetate, sodium 2-methyl-4-chlorophenoxyacetate, ethyl 2-methyl-4-chlorophenoxyacetate, butyl 2-methyl-4-chlorophenoxyacetate, sodium 2-methyl-4-chlorophenoxybutyrate, The herbicidal composition according to any one of claims 1 to 3, wherein the composition is ethyl 2-methyl-4-chlorophenoxybutyrate or potassium α- (2-methyl-4-chlorophenoxy) propionate. 6. A method for controlling weeds, comprising spraying the herbicidal composition according to claim 1 on weeds. 7. Weed control characterized by spraying a weed with a herbicide containing a microorganism having the ability to control weeds as an active ingredient and a herbicide containing a phenoxycarboxylic acid derivative as an active ingredient. Method.