KR102652958B1 - Apparatus for heating and cooling samples and method for heating and cooling samples using the same - Google Patents

Abstract

The present invention relates to a sample heating and cooling device and a sample heating and cooling method using the same. The sample heating and cooling device according to an embodiment of the present invention includes a container for receiving a sample, magnetic particles included in the sample, and It is arranged to cover the container and includes a coil part that generates heat in the magnetic particles by generating a magnetic field to heat the sample, and a cooling part that cools the sample.

Description

Sample heating and cooling device and sample heating and cooling method using the same {APPARATUS FOR HEATING AND COOLING SAMPLES AND METHOD FOR HEATING AND COOLING SAMPLES USING THE SAME}

The present invention relates to a sample heating and cooling device and a sample heating and cooling method using the same, and more specifically, to a device and method for heating a sample using heat generation from magnetic particles.

In the field of biotechnology, especially genetic diagnosis, it is very important to control the temperature of the sample by heating and cooling the sample.

For example, polymerase chain reaction (PCR) testing is a reaction that amplifies a specific target genetic material of interest, and uses enzymes to continuously replicate template DNA. The steps of the polymerase chain reaction (PCR) are generally the denaturation step, which unwinds the double-stranded template DNA to be copied into a single strand, designates the place where the reaction will start on the unraveled single strand, and helps start the enzyme reaction. It is divided into three stages: an annealing stage, which combines primers, and an extension stage, which replicates DNA from the position where the primer is attached to create DNA with a complete double helix structure. Carrying out the above three steps theoretically doubles the amount of DNA, and by repeating this process n times, the amount of DNA theoretically increases to the nth power of 2.

Generally, each step is required to be controlled to a specific temperature, such as the denaturation step at about 90 to 96°C, the annealing step at about 60°C, and the extension step at 72°C. For this purpose, the temperature of the sample is controlled through a sample heating and cooling device that uses various methods, such as using Peltier elements. In addition to polymerase chain reaction (PCR), sample temperature control is also a very important factor in in-vitro protein translation and cell-free protein expression.

However, these sample heating and cooling devices generally have difficulty heating and cooling samples at low power. In addition, since power is generally supplied directly through a conductor connected to a power source, it is difficult to use outdoors and has poor portability.

Accordingly, there was a demand for devices and methods that can heat and cool samples with low power. Additionally, there was a demand for a sample heating and cooling device and method that could improve portability using wireless power transmission.

The above-mentioned background technology is technical information that the inventor possessed for deriving the present invention or acquired in the process of deriving the present invention, and cannot necessarily be said to be known technology disclosed to the general public before filing the application for the present invention.

The present invention provides a device and method for heating and cooling a sample at low power by using the heat generation of magnetic particles, especially magnetic hysteresis loss. In addition, it provides a sample heating and cooling device and method that can be driven with wireless power and thus has improved portability.

However, these problems are illustrative, and the problems to be solved by the present invention are not limited thereto.

A sample heating and cooling device according to an embodiment of the present invention is disposed to cover a container containing a sample, magnetic particles contained in the sample, and the container, and generates a magnetic field to heat the sample to affect the magnetic particles. It includes a coil part that generates heat and a cooling part that cools the sample.

In the sample heating and cooling device according to an embodiment of the present invention, the magnetic particles may generate heat through magnetic hysteresis loss caused by a magnetic field generated by the coil unit.

In the sample heating and cooling device according to an embodiment of the present invention, the magnetic particles are Fe, Co, Mg, Mn, Ni, Zn, Fe-Mn, Co-Ti, Mn-Mg, Zn-Fe, Zn- It may be a metal itself containing at least one of Ca, Mn-Zn, Co-Zn, and Ni-Zn metals, an alloy thereof, or an oxide thereof.

In the sample heating and cooling device according to an embodiment of the present invention, the magnetic particles are Fe, Co, Mg, Mn, Ni, Zn, Fe-Mn, Co-Ti, Mn-Mg, Zn-Fe, Zn- It may be made by using at least one of metals such as Ca, Mn-Zn, Co-Zn, and Ni-Zn as a core and doping the oxide of the metal as a shell.

In the sample heating and cooling device according to an embodiment of the present invention, the power supply unit may be disposed outside the sample heating and cooling device to wirelessly transmit power.

In the sample heating and cooling device according to an embodiment of the present invention, the coil unit may include a solenoid coil, and the container may be disposed in an internal space formed by the coil unit.

In the sample heating and cooling device according to an embodiment of the present invention, the coil unit includes a screw coil, and the container may be disposed above or below the coil unit.

In the sample heating and cooling device according to an embodiment of the present invention, the containers may be formed in plural pieces, and the coil portion may be formed as one to cover all of the plurality of containers.

In the sample heating and cooling device according to an embodiment of the present invention, the containers may be formed in plural pieces, and the coil portion may be formed in plural pieces to cover each of the plurality of containers.

A sample heating and cooling device according to an embodiment of the present invention includes a container for accommodating a sample, a heating portion configured to surround the outside of the container, and a heating portion for accommodating a heating fluid containing magnetic particles, and a heating portion for covering the heating portion. It is disposed and includes a coil part that generates heat in the magnetic particles by generating a magnetic field to heat the sample, and a cooling part that cools the sample.

In the sample heating and cooling device according to an embodiment of the present invention, the magnetic particles may generate heat through magnetic hysteresis loss caused by a magnetic field generated by the coil unit.

A sample heating and cooling method according to an embodiment of the present invention is a sample heating and cooling method using a sample heating and cooling device including a sample, magnetic particles, a coil unit, a cooling unit, a power unit, a cooling unit, and a control unit, comprising magnetic particles. receiving a sample containing a sample in a container, applying power by a power supply unit to generate a magnetic field in the coil unit, heating the sample by causing the magnetic particles to generate heat by the magnetic field, and heating the sample by a cooling unit. It includes the step of cooling.

In the sample heating and cooling method according to an embodiment of the present invention, the step of heating the sample includes generating heat through magnetic hysteresis loss caused by the magnetic field generated by the coil unit. It can be included.

In the sample heating and cooling method according to an embodiment of the present invention, the power supply unit may further include transmitting power wirelessly.

Other aspects, features and advantages other than those described above will become apparent from the detailed description, claims and drawings for carrying out the invention below.

The sample heating device and method according to an embodiment of the present invention generates heat by applying a magnetic field to the magnetic particles contained in the sample, and uses this heat to heat the sample, thereby heating the sample with low power, that is, high efficiency. there is.

The sample heating device and method according to an embodiment of the present invention receives power wirelessly, providing a sample heating and cooling device that has improved portability and is highly usable outdoors.

The effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.

Figure 1 shows a sample heating and cooling device according to an embodiment of the present invention.
FIG. 2 shows that the container and coil portion in FIG. 1 are formed in plural pieces.
Figure 3 shows a sample heating and cooling device according to another embodiment of the present invention.
FIG. 4 shows that the container and coil portion in FIG. 3 are formed in plural pieces.
Figure 5 shows the hysteresis curve.
Figure 6 shows the amount of heat generated by magnetic particles depending on the size of the magnetic particles.
Figure 7 shows that the power supply unit includes a wireless power transmission unit according to an embodiment of the present invention.
Figure 8 shows a sample heating and cooling device according to another embodiment of the present invention.
Figure 9 is a conceptual diagram schematically showing a sample heating and cooling method according to an embodiment of the present invention.

Since the present invention can be modified in various ways and can have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the description of the invention. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all transformations, equivalents, and substitutes included in the spirit and technical scope of the present invention. In describing the present invention, the same identification numbers are used for the same components even if they are shown in different embodiments.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. When describing with reference to the drawings, identical or corresponding components will be assigned the same reference numerals and redundant description thereof will be omitted. .

In the following embodiments, terms such as first and second are used not in a limiting sense but for the purpose of distinguishing one component from another component.

In the following examples, singular terms include plural terms unless the context clearly dictates otherwise.

In the following embodiments, terms such as include or have mean that the features or components described in the specification exist, and do not exclude in advance the possibility of adding one or more other features or components.

In the drawings, the sizes of components may be exaggerated or reduced for convenience of explanation. For example, the size and thickness of each component shown in the drawings are shown arbitrarily for convenience of explanation, so the present invention is not necessarily limited to what is shown.

In the following embodiments, the x-axis, y-axis, and z-axis are not limited to the three axes in the Cartesian coordinate system, but can be interpreted in a broad sense including these. For example, the x-axis, y-axis, and z-axis may be orthogonal to each other, but may also refer to different directions that are not orthogonal to each other.

In cases where an embodiment can be implemented differently, a specific process sequence may be performed differently from the described sequence. For example, two processes described in succession may be performed substantially at the same time, or may be performed in an order opposite to the order in which they are described.

The terms used in this application are only used to describe specific embodiments and are not intended to limit the invention. In this application, terms such as “comprise” or “have” are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features. It should be understood that this does not exclude in advance the possibility of the existence or addition of elements, numbers, steps, operations, components, parts, or combinations thereof.

Hereinafter, a sample heating and cooling device 10 according to an embodiment of the present invention will be described with reference to the drawings.

FIG. 1 shows a sample heating and cooling device 10 according to an embodiment of the present invention, and FIG. 2 shows a plurality of containers 100 and coil units 300 in FIG. 1 . FIG. 3 shows a sample heating and cooling device 10 according to another embodiment of the present invention, and FIG. 4 shows that the container 100 and the coil unit 300 in FIG. 3 are formed in plural pieces.

The sample heating and cooling device 10 according to an embodiment of the present invention can be used to heat and cool samples in the biotechnology field, especially in polymerase chain reaction. However, it is not limited to this, and can be used to control the temperature of the sample by heating and cooling the sample in other tests or experiments such as in vitro protein translation and cell-free protein expression.

Referring to FIG. 1, the sample heating and cooling device 10 according to an embodiment of the present invention includes a container 100, magnetic particles 200, a coil unit 300, a cooling unit 400, and a power unit 500. , may include a control unit 600 and a sensor unit 700.

The container 100 is a rigid body with a cavity formed inside to accommodate a sample, and may have a shape suitable for accommodating a fluid-type sample, which is a common sample phase. A sample refers to a material used for scientific testing, inspection, chemical analysis, etc. for a specific purpose, and may include, for example, DNA that is subject to amplification in a polymerase chain reaction.

The container 100 may be a single container 100 that accommodates one sample, or may be composed of a plurality of containers 100 that accommodate a plurality of samples. When a plurality of containers 100 are formed, a plurality of separate containers 100 may each accommodate a plurality of samples. Alternatively, the plurality of containers 100 may be formed by forming a plurality of concave portions to accommodate a plurality of samples in one rack.

The interior of the container 100 may accommodate a sample, more specifically, a sample in the form of a fluid, and the sample may include magnetic particles 200.

Magnetic particles (200) are ferromagnetic materials that are magnetized without an external magnetic field or paramagnetic materials that are magnetized by an external magnetic field. In one embodiment, the magnetic particles (200) are magnetic micro particles (magnetic micro particles) or magnetic nanoparticles ( It may be a magnetic nano particle). Among magnetic particles, magnetic microparticles are those whose size is on the order of micrometers (μm), that is, 10 ^-6 meters (m). Among magnetic particles, magnetic nanoparticles mean the size of nanometers (nm), that is, at the level of 10 ^-9 meters (m).

Magnetic particles 200, especially magnetic nanoparticles 200, can be mixed with a sample and generate heat in response to the magnetic field of the coil unit 300, as will be described later. Specifically, the magnetic particles 200 may generate heat by magnetic hysteresis loss in response to the magnetic field of the coil unit 300. The heat generated by the magnetic particles 200 may be transferred to the sample and increase the temperature of the sample.

In one embodiment, the magnetic particles 200 include Fe, Co, Mg, Mn, Ni, Zn, Fe-Mn, Co-Ti, Mn-Mg, Zn-Fe, Zn-Ca, Mn-Zn, Co-Zn, It may be a metal itself containing at least one of Ni-Zn metals, an alloy thereof, or an oxide thereof.

In one embodiment, the magnetic particles 200 include Fe, Co, Mg, Mn, Ni, Zn, Fe-Mn, Co-Ti, Mn-Mg, Zn-Fe, Zn-Ca, Mn-Zn, Co-Zn, The core may be made of at least one of Ni-Zn metals or an alloy thereof, and the outer surface may be subjected to surface chemical treatment. Surface chemical treatment can be implemented, for example, by doping the outer surface of the core with an oxide of the metal or its alloy or coating the outer surface of the core with a polymer compound. However, it is not limited to this and various methods for preventing oxidation can be used. Accordingly, the magnetic particles 200 can prevent the problem of being easily oxidized compared to the case where the magnetic particles 200 are made of only the metals listed above or their alloys.

In one embodiment, the magnetic particles 200 may be made of a perovskite structure based on Mn, an Fe-Co alloy, or a Cu-Ni alloy.

Although not shown in the drawing, an insulating material may be disposed on the outside of the container 100 containing the magnetic particles 200 and the sample to surround the container 100. Accordingly, when the magnetic particles 200 generate heat by the coil unit 300, which will be described later, heat transfer to the outside can be minimized and the heat can be completely transferred to the inside of the container 100, that is, to the sample.

Meanwhile, the coil unit 300 is a member that can generate a magnetic field when power is applied, and may be arranged to cover the container 100 containing the sample and the magnetic particles 200.

The coil unit 300 may generate a magnetic field by power applied from the power supply unit 500, which will be described later. At this time, the magnetic field may be an alternating magnetic field. The alternating magnetic field generated by the coil unit 300 can cause the magnetic particles 200 to generate heat. Specifically, magnetic particles 200, especially magnetic nanoparticles 200, can generate heat due to magnetic hysteresis loss.

Figure 5 shows a magnetic hysteresis curve, and Figure 6 shows the amount of heat generated by the magnetic particles 200.

Referring to FIG. 5, the magnetic hysteresis loss is caused by the path difference between the magnetization and demagnetization characteristic curves due to changes in the external magnetic field during the process in which the magnetic particles 200 are repeatedly magnetized and demagnetized in the direction of the magnetic field under the influence of the magnetic flux in the alternating magnetic field. This is a phenomenon in which heat is generated. In other words, when the magnetic flux in the alternating magnetic field is repeated once, the energy loss corresponding to the area surrounded by the magnetic hysteresis curve of FIG. 5 is shown. The amount of heat energy generated at this time is proportional to the area surrounded by the hysteresis curve expressed by the following formula.

At this time, P is the amount of energy per time (second), is the vacuum magnetic permeability, is the applied frequency, H is the external magnetic field, and M is magnetization.

When the magnetic particles 200 are included in the sample, they can generate heat due to magnetic hysteresis loss and heat the sample under the influence of the alternating magnetic field generated by the coil unit 300. Accordingly, compared to existing heat generation methods using eddy current or Peltier elements, it can be driven with lower power and heat generation efficiency can be improved.

Additionally, the magnetic particles 200 may generate heat through relaxation. The relaxation phenomenon may occur depending on the size and anisotropy of the magnetic particles 200, especially the magnetic nanoparticles 200. The relaxation phenomenon occurs when the magnetic nanoparticles (200) are fixed and the super spin direction of the magnetic nanoparticles (200) is aligned parallel to the direction of the external magnetic field, and heat is generated by energy that tries to disrupt the realignment of the super spins. It could be (neel relaxation). The relaxation phenomenon occurs when the magnetic nanoparticles (200) move freely in a fluid, and the super spin has the same direction as the crystal structure of the magnetic nanoparticles (200) and aligns with the external magnetic field. At this time, between the fluid and the magnetic nanoparticles. This may be Brown relaxation, in which heat is generated by friction caused by shear stress.

The sample heating and cooling device 10 according to the present invention uses magnetic particles 200, especially magnetic nanoparticles 200, so that it can be driven at low power not only through magnetic hysteresis loss but also through heat generation due to relaxation phenomenon. You can. Figure 6 shows the heat generated depending on the size of the magnetic particle, specifically the heat due to magnetic hysteresis loss and the heat due to the relaxation phenomenon. In this way, the magnetic particles 200 can generate heat equal to the total amount of heat generated by magnetic hysteresis loss and relaxation phenomenon.

Referring again to FIGS. 1 to 4 , the coil portion 300 may be arranged to cover the container 100 . Specifically, the coil unit 300 may be disposed adjacent to the container 100 and cover the container 100 so as to apply a magnetic field to the magnetic particles 200 accommodated inside the container 100.

In one embodiment, the coil unit 300 may include a solenoid coil as shown in FIG. 1. At this time, the container 100 may be placed in the internal space formed by the coil unit 300. Additionally, the central axis of the coil unit 300, specifically the solenoid coil, and the longitudinal axis of the container 100 may be arranged to be parallel. Accordingly, the magnetic field generated from the coil unit 300 can equally apply magnetic force to the magnetic particles 200 in the container 100.

In one embodiment, the coil unit 300 may include a spiral coil as shown in FIG. 3. At this time, the container 100 may be placed on or below the coil unit 300. Additionally, the central axis of the coil unit 300, specifically the spiral coil, may be arranged to be parallel to the longitudinal axis of the container 100. Accordingly, the magnetic field generated from the coil unit 300 can equally apply magnetic force to the magnetic particles 200 in the container 100.

1 and 3 show that the coil unit 300 includes a soleroid coil and a screw coil, but it is not limited thereto and may include various types of coils capable of applying a magnetic field to the magnetic particles 200. You will understand.

Also, referring to FIGS. 1 and 3 , in one embodiment, when the coil unit 300 is formed in plural containers 100, the coil unit 300 may be formed as one coil that covers all of the plurality of containers 100. When the coil unit 300 includes a solenoid coil as shown in FIG. 1, the radius of the coil unit 300 may be determined to have an internal space that can accommodate all of the plurality of containers 100. When the coil unit 300 includes a threaded coil as shown in FIG. 2, a coil unit 300 with a larger radius than the container 100 is installed at the top or bottom of the container 100 to cover all of the plurality of containers 100. can be placed. Accordingly, the sample heating and cooling device 10 according to the present invention is economical because only one coil unit 300 can be disposed for a plurality of containers 100, and space for arrangement of each component can be saved. .

Referring to FIGS. 2 and 4 , in one embodiment, when the plurality of containers 100 are formed, the coil portion 300 may be formed in plural pieces to cover each of the plurality of containers 100 . When the coil unit 300 includes a solenoid coil as shown in FIG. 2, each of the plurality of containers 100 may be disposed in an internal space formed by each of the plurality of coil parts 300. When the coil unit 300 includes a screw coil as shown in FIG. 4, each of the plurality of containers 100 may be disposed within a radius of the coil unit 300 at the top or bottom of the plurality of coil units 300. Accordingly, the sample heating and cooling device 10 according to the present invention can control the temperatures of a plurality of samples contained in each of the plurality of containers 100. Specifically, the sample heating and cooling device 10 can control the temperature of a plurality of samples by controlling the power supply to each of the plurality of coil units 300.

The cooling unit 400 is disposed adjacent to the container 100 and can cool the container 100, particularly the sample contained within the container 100. In one embodiment, when the coil unit 300 is a solenoid coil, the container 100 may be placed in the internal space formed by the coil unit 300, and the cooling unit 400 may be placed at the top or bottom of the container 100. there is. In one embodiment, when the coil unit 300 is a screw coil, the container 100 is disposed at either the top or bottom of the coil unit 300, and the cooling unit 400 is located at either the top or bottom of the coil unit 300. Can be placed on one or the other. However, it is understood that the cooling unit 400 is not limited thereto and may be disposed adjacent to the container 100 to cool the container 100, such as being disposed between the coil unit 300 and the container 100.

In one embodiment, the cooling unit 400 may be a thermoelectric cooling method using a Peltier element. Specifically, the cooling unit 400 can cool the container 100, particularly the sample contained in the container 100, by allowing the current supplied from the power supply 500 to flow through the Peltier element.

In one embodiment, the cooling unit 400 may be an air cooling method using a fan. Specifically, the cooling unit 400 may include a forced fan or an induced fan. The cooling unit 400 causes air to flow through a press-fit fan or an induced fan, and the air can cool the container 100, particularly the sample contained in the container 100. At this time, the container 100 may be formed in a wrinkled shape to secure a contact area with air.

Figure 7 shows that the power supply unit 500 includes a wireless power transmission unit according to an embodiment of the present invention.

The power supply unit 500 is disposed inside the sample heating and cooling device 10 and can supply power to parts that require power, such as the coil unit 300 and the cooling unit 400.

Alternatively, in one embodiment, the power supply unit 500 may include a wireless power transmission unit. At this time, the power supply unit 500 may be disposed outside the sample heating and cooling device 10. The power supply unit 500, specifically the wireless power transmission unit, can wirelessly supply power to the sample heating and cooling device 10 through the transmission coil 520 from outside the sample heating and cooling device 10. At this time, the sample heating and cooling device 10 may further include a receiving coil 800 capable of receiving power transmitted from the wireless power transmitter.

The wireless power transmitter can transmit power using magnetic induction. The wireless power transmitter generates a magnetic field through the transmitting coil 520, and the receiving coil 800 can receive power as an induced current flows therefrom.

Alternatively, the wireless power transmitter may transmit power using a magnetic resonance method. The transmitting coil 520 generates a magnetic field that oscillates at a resonant frequency, and the receiving coil 800 is designed at the same resonant frequency to receive power.

Since magnetic induction or magnetic resonance wireless power transmission is similar to generally known methods, detailed descriptions are omitted.

The sample heating and cooling device 10 according to the present invention has a power supply unit 500 that can transmit power wirelessly from the outside of the sample heating and cooling device 10, improving portability compared to devices that supply power by wire. It can be. In addition, the sample heating and cooling device 10 can be used by supplying power wirelessly even outdoors where wired power supply is not smooth. Additionally, since power is supplied wirelessly, it does not require a built-in battery, making the device 10 lighter.

The control unit 600 can control the operation of the coil unit 300 and the cooling unit 400 in the sample heating and cooling device 10. Specifically, the control unit 600 can control the power supplied to the coil unit 300 and the cooling unit 400 to control the amount of heat applied to the container 100, particularly the sample contained in the container 100. For example, when the temperature of the sample needs to be increased, the control unit 600 can increase the amount and frequency of power supplied to the coil unit 300 to further increase the amount of heat generated by the magnetic particles 200. Alternatively, when the temperature of the sample needs to be lowered, the control unit 600 can stop the operation of the coil unit 300 and drive the cooling unit 400 to lower the temperature of the sample.

In this way, the control unit 600 receives temperature information of the container 100 or the sample from the sensor unit 700, which will be described later, and controls the operation of the coil unit 300 and the cooling unit 400 to adjust the temperature of the sample to the required temperature. It can be controlled with .

The sensor unit 700 can detect signals coming from the container 100 and the sample contained in the container 100 using an optical or electrochemical method. The sensor unit 700 may be placed adjacent to the container 100, for example, at the top or bottom of the container 100. However, it is not limited thereto, and may be placed at various locations adjacent to the container 100 to detect signals coming from the sample accommodated in the container 100.

In one embodiment, the sensor unit 700 includes a temperature sensor, and the temperature sensor is disposed adjacent to the container 100 to detect temperature information of the container 100. The sensor unit 700 detects the temperature of the container 100 and transmits it to the control unit 600, and the control unit 600 can estimate the temperature of the sample contained in the container 100 through correction.

In one embodiment, the sensor unit 700 includes an optical sensor including a light irradiation unit and a light detection unit. The light irradiation unit may irradiate light to the sample, and the light detection unit may detect light from the sample. An optical sensor can measure the decrease in light intensity by passing light of a specific wavelength through a sample, and through this, the concentration of DNA can be measured.

The sample heating and cooling device 10 according to an embodiment of the present invention described above generates heat by applying a magnetic field to the magnetic particles contained in the sample, and uses this heat to heat the sample with low power, that is, high efficiency. The sample can be heated. In addition, it is possible to provide a sample heating and cooling device 10 that receives power wirelessly, improves portability, and is highly usable outdoors.

Figure 8 is a perspective view showing a sample heating and cooling device 10' according to another embodiment of the present invention.

Referring to FIG. 8, a sample heating and cooling device 10' according to an embodiment of the present invention includes a container 100', magnetic particles 200', a heating unit 900', a coil unit 300', It may include a cooling unit 400', a power unit 500', a control unit 600', and a sensor unit 700'. Hereinafter, the description will focus on the differences from the above-described embodiment.

The container 100' is a rigid body with a cavity formed inside to accommodate a sample, and may have a shape suitable for accommodating a fluid-type sample, which is a common sample phase.

The container 100' may be a single container 100' accommodating one sample, or may be composed of a plurality of containers 100' accommodating a plurality of samples.

A sample, more specifically a sample in the form of a fluid, may be accommodated inside the container 100'.

The heating unit 900' may be arranged to surround the outside of the container 100'. Specifically, the heating unit 900' may have a cylindrical shape surrounding the outside of the container 100' to transfer heat to the container 100' and the sample contained in the container 100'.

A heating fluid containing magnetic particles 200' may be accommodated in the internal space of the heating unit 900'. The heating fluid includes magnetic particles 200', and when the magnetic particles 200' generate heat by the magnetic field of the coil unit 300', the heating fluid is a medium fluid for receiving the heat and transferring it to the container 100'. am. In one embodiment, the heating fluid may be an aqueous solution containing magnetic particles 200'. Alternatively, in one embodiment, the heating fluid may be a fluid whose temperature can easily change due to a small specific heat. However, without being limited thereto, various fluids that can receive heat from the magnetic particles 200' and transfer heat to the container 100' may be adopted.

The heating unit 900' is shown in FIG. 8 as having a cylindrical shape surrounding the outside of the container 100', but is not limited thereto. For example, the heating unit 900' surrounds the outside of the container 100' and may be formed in the shape of a polygonal pillar, such as a square pillar. For example, the heating unit 900' may be formed in a ring shape surrounding the container 100' and may be arranged in plural numbers spaced apart along the longitudinal direction of the container 100'.

In one embodiment, the heating unit 900' is formed in a ring shape surrounding the container 100', and the first heating unit, the second heating unit, and the second heating unit are arranged to be spaced apart along the longitudinal direction of the container 100'. 3 It may include a heating unit. The first heating unit, the second heating unit, and the third heating unit may each include different types of magnetic particles 200'. Alternatively, the first heating unit, the second heating unit, and the third heating unit may include magnetic particles 200' of the same type and accommodate first heating fluid, second heating fluid, and third heating fluid having different specific heats, respectively. You can. As a result, the heat generated under the influence of the magnetic field can be differentiated according to each heating unit 900'. This can heat the container 100' containing the sample at partially different heating amounts.

In this way, the sample heating and cooling device 10' includes a heating unit 900', so that the sample and the magnetic particles 200' can be placed separately. That is, since the magnetic particles 200' are not included in the sample but are included in the heating fluid, the sample heating and cooling device 10' can perform experiments or tests in which other substances must not be mixed with the sample.

Magnetic particles 200', especially magnetic nanoparticles 200', can be mixed with the heating fluid in the heating unit 900' and generate heat in response to the magnetic field of the coil unit 300', as will be described later. . Specifically, the magnetic particles 200' may respond to the magnetic field of the coil unit 300' and generate heat due to magnetic hysteresis loss. The heat generated by the magnetic particles 200' is transferred to the heating fluid, raising the temperature of the heating fluid, and the heating fluid can transfer heat back to the container 100'. Accordingly, the temperature of the sample can be increased.

In one embodiment, the magnetic particles 200' include Fe, Co, Mg, Mn, Ni, Zn, Fe-Mn, Co-Ti, Mn-Mg, Zn-Fe, Zn-Ca, Mn-Zn, Co-Zn. , it may be a metal itself containing at least one of Ni-Zn metals, an alloy thereof, or an oxide thereof.

In one embodiment, the magnetic particles 200 include Fe, Co, Mg, Mn, Ni, Zn, Fe-Mn, Co-Ti, Mn-Mg, Zn-Fe, Zn-Ca, Mn-Zn, Co-Zn, The core may be made of at least one of Ni-Zn metals or an alloy thereof, and the outer surface may be subjected to surface chemical treatment. Surface chemical treatment can be implemented, for example, by doping the outer surface of the core with an oxide of the metal or its alloy or coating the outer surface of the core with a polymer compound. In one embodiment, the magnetic particles 200' may be made of a perovskite structure based on Mn, an Fe-Co alloy, or a Cu-Ni alloy.

An insulating material 110' may be disposed outside the heating unit 900' to surround the heating unit 900'. Accordingly, when the magnetic particles 200' generate heat by the coil part 300', which will be described later, heat transfer to the outside is minimized and the heat is transferred to the heating part 900' inside the insulating material 110' and the container 100'. ) and can be completely delivered as a sample.

Meanwhile, the coil unit 300' is a member capable of generating a magnetic field and may be arranged to cover the container 100' and the heating unit 900'.

The coil unit 300' may generate a magnetic field by power applied from the power unit 500'. At this time, the magnetic field may be an alternating magnetic field. The alternating magnetic field generated by the coil unit 300' can cause the magnetic particles 200' to generate heat. Specifically, magnetic particles 200', especially magnetic nanoparticles 200', can generate heat due to magnetic hysteresis loss.

In one embodiment, the coil unit 300' may include a solenoid coil. At this time, the container 100' and the heating unit 900' may be placed in the internal space formed by the coil unit 300'.

In one embodiment, the coil unit 300' may include a screw coil. At this time, the container 100' and the heating unit 900' may be disposed above or below the coil unit 300'.

In one embodiment, the coil unit 300' may be formed as one coil that covers all of the plurality of containers 100' when the containers 100' are formed in plural pieces. When the coil unit 300' includes a solenoid coil, the radius of the coil unit 300' may be determined to have an internal space that can accommodate all of the plurality of containers 100'. When the coil portion 300' includes a threaded coil, a coil portion 300' having a larger radius than the container 100' is provided at the top or bottom of the container 100' to cover all of the plurality of containers 100'. can be placed.

In one embodiment, when the containers 100' are formed in plural pieces, the coil portions 300' may be formed in plural pieces to cover each of the plurality of containers 100'. When the coil unit 300' includes a solenoid coil, each of the plurality of containers 100' may be disposed in an internal space formed by each of the plurality of coil units 300'. When the coil unit 300' includes a screw coil, each of the plurality of containers 100' may be disposed within a radius of the coil unit 300' at the top or bottom of the plurality of coil units 300'.

The cooling unit 400' is disposed adjacent to the container 100' and can cool the container 100', particularly the sample contained within the container 100'. In one embodiment, when the coil part 300' is a solenoid coil, the container 100' is disposed in the internal space formed by the coil part 300', and the cooling part 400' is located at the top or bottom of the container 100'. ) can be placed. In one embodiment, when the coil unit 300' is a screw coil, the container 100' is disposed at either the upper or lower part of the coil unit 300', and the cooling unit 400' is located on the coil unit 300'. It may be placed in either the upper or lower part of the .

In one embodiment, the cooling unit 400' may be a thermoelectric cooling method using a Peltier element. In one embodiment, the cooling unit 400 may be an air cooling method using a fan.

The power supply unit 500' is disposed inside the sample heating and cooling device 10' and can supply power to parts that require power, such as the coil unit 300' and the cooling unit 400'.

Alternatively, in one embodiment, the power unit 500' may include a wireless power transmission unit. At this time, the power supply unit 500' may be disposed outside the sample heating and cooling device 10'. The power supply unit 500', specifically the wireless power transmission unit, can wirelessly supply power to the sample heating and cooling device 10' through a transmission coil from outside the sample heating and cooling device 10'. At this time, the sample heating and cooling device 10' may further include a receiving coil 800' capable of receiving power transmitted from the wireless power transmitter.

The control unit 600' can control the operation of the coil unit 300' and the cooling unit 400' in the sample heating and cooling device 10'. Specifically, the control unit 600' controls the power supplied to the coil unit 300' and the cooling unit 400' to control the amount of heat applied to the container 100', especially the sample contained in the container 100'. You can.

The sensor unit 700' can detect signals coming from the container 100' and the sample contained in the container 100' using an optical or electrochemical method. The sensor unit 700' may be placed adjacent to the container 100', for example, at the top or bottom of the container 100'.

In one embodiment, the sensor unit 700' includes a temperature sensor, and the temperature sensor is disposed adjacent to the container 100' to detect temperature information of the container 100'. The sensor unit 700' detects the temperature of the container 100' and transmits it to the control unit 600', and the control unit 600' can estimate the temperature of the sample contained in the container 100' through correction. .

In one embodiment, the sensor unit 700' includes an optical sensor including a light irradiation unit and a light detection unit. The light irradiation unit may irradiate light to the sample, and the light detection unit may detect light from the sample. An optical sensor can measure the decrease in light intensity by passing light of a specific wavelength through a sample, and through this, the concentration of DNA can be measured.

The sample heating and cooling device 10' according to an embodiment of the present invention described above includes a heating unit 900', and uses heat generated by magnetic hysteresis loss of the magnetic particles 200' while maintaining the magnetic particles 200'. ') can be arranged so that it is separated from the sample.

Figure 9 is a conceptual diagram schematically showing a sample heating and cooling method (S10) according to an embodiment of the present invention.

Referring to FIG. 9, the sample heating and cooling method (S10) according to an embodiment of the present invention may use the sample heating and cooling device 10. However, it will be understood that the sample heating and cooling method (S10) is not limited to this and may be performed by another device or mechanism using the magnetic particles 200.

In the sample heating and cooling method (S10), a sample containing magnetic particles 200 may first be accommodated in a container (S100).

Next, power is applied by the power supply unit 500 so that the coil unit 300 can generate a magnetic field (S200). At this time, the coil unit 300 may generate an alternating magnetic field.

The magnetic particles 200 included in the sample may generate heat under the influence of the magnetic field of the coil unit 300 (S300). Specifically, the magnetic particles 200 may respond to the alternating magnetic field of the coil unit 300 and generate heat due to magnetic hysteresis loss. The heat generated by the magnetic particles 200 may be transferred to the sample and increase the temperature of the sample.

In order to lower the temperature of the sample, the sample can be cooled by the cooling unit 400 (S400). The cooling unit 400 is disposed adjacent to the container 100 and can cool the container 100, particularly the sample contained within the container 100. In one embodiment, the cooling unit 400 may cool the sample using a thermoelectric cooling method using a Peltier element. In one embodiment, the cooling unit 400 may cool the sample using an air cooling method using a fan.

The operation of the coil unit 300 and the cooling unit 400 are controlled by the control unit 600, thereby controlling the temperature of the sample (S500). Specifically, the control unit 600 can control the power supplied to the coil unit 300 and the cooling unit 400 to control the amount of heat applied to the container 100, particularly the sample contained in the container 100. For example, when the temperature of the sample needs to be increased, the control unit 600 can increase the power supplied to the coil unit 300 to further increase the amount of heat generated by the magnetic particles 200. Alternatively, when the temperature of the sample needs to be lowered, the control unit 600 may stop driving the coil unit 300 and drive the cooling unit 400 to lower the temperature of the sample.

In the sample heating and cooling method (S10) according to an embodiment of the present invention, the power supply unit 500 may further include a step of wirelessly transmitting power. The power supply unit 500 can supply power to parts that require power, such as the coil unit 300 and the cooling unit 400. Alternatively, in one embodiment, the power supply unit 500 may include a wireless power transmission unit. At this time, the power supply unit 500 may be disposed outside the sample heating and cooling device 10. The power supply unit 500, specifically the wireless power transmission unit, can wirelessly supply power to the sample heating and cooling device 10 through the transmission coil 520 from outside the sample heating and cooling device 10. At this time, the sample heating and cooling device 10 may further include a receiving coil 800 capable of receiving power transmitted from the wireless power transmitter.

The wireless power transmitter can transmit power using a magnetic induction method or a magnetic resonance method.

As described above, the sample heating and cooling method (S10) according to an embodiment of the present invention applies a magnetic field to the magnetic particles 200 included in the sample to generate heat, and uses this heat to heat the sample. This allows the sample to be heated with low power, that is, high efficiency.

In addition, by supplying power wirelessly, samples can be easily heated and cooled even outdoors where power supply is poor.

Figure 10 shows a heating curve of a fluid conducted according to an embodiment of the present invention.

Specifically, the fluid was heated using the sample heating and cooling device shown in FIG. 1, and the temperature rise due to heating is shown.

20 μl of deionized water was used as the sample, and iron oxide with a diameter of 2.8 μm was used as the magnetic particle. As shown in FIG. 10, it can be confirmed that as power is input, the magnetic particles generate heat, which gradually increases the temperature of the sample.

Although the present invention has been described with reference to the embodiments shown in the drawings, these are merely examples. Those skilled in the art can fully understand that various modifications and equivalent other embodiments are possible from the embodiments. Therefore, the true technical protection scope of the present invention should be determined based on the attached claims.

The specific technical content described in the embodiment is an example and does not limit the technical scope of the embodiment. In order to describe the invention concisely and clearly, descriptions of conventional general techniques and configurations may be omitted. In addition, the connections or absence of connections between the components shown in the drawings exemplify functional connections and/or physical or circuit connections, and in actual devices, various functional connections or physical connections may be replaced or added. It can be expressed as connections, or circuit connections. Additionally, if there is no specific mention such as “essential,” “important,” etc., it may not be a necessary component for the application of the present invention.

“The” or similar designators used in the description and claims may refer to both the singular and the plural, unless otherwise specified. In addition, when a range is described in an example, the invention includes the application of individual values within the range (unless there is a statement to the contrary), and each individual value constituting the range is described in the description of the invention. same. Additionally, unless the order of the steps constituting the method according to the embodiment is clearly stated or there is no description to the contrary, the steps may be performed in an appropriate order. The embodiments are not necessarily limited by the order of description of the steps above. The use of any examples or illustrative terms (e.g., etc.) in the embodiments is merely to describe the embodiments in detail, and unless limited by the claims, the examples or illustrative terms do not limit the scope of the embodiments. That is not the case. Additionally, those skilled in the art will appreciate that various modifications, combinations and changes may be made according to design conditions and factors within the scope of the appended claims or their equivalents.

10: Sample heating and cooling device 100: Container
200: magnetic particle 300: coil part
400: Cooling unit 500: Power unit
600: Control unit 700: Sensor unit
800: Receiving coil 900: Heating unit

Claims (10)

A container for receiving a sample;
Magnetic particles contained in the sample;
a coil portion disposed to cover the container and generating heat in the magnetic particles by generating a magnetic field to heat the sample;
a cooling unit that cools the sample;
A power supply unit disposed externally and supplying power wirelessly through a transmission coil; and
A sample heating and cooling device comprising: a receiving coil for receiving power transmitted from the transmitting coil. According to paragraph 1,
A sample heating and cooling device in which the magnetic particles generate heat through magnetic hysteresis loss caused by a magnetic field generated by the coil unit. According to paragraph 1,
The coil unit includes a solenoid coil,
A sample heating and cooling device wherein the container is disposed in an internal space formed by the coil unit. According to paragraph 1,
The coil unit includes a screw coil,
A sample heating and cooling device, wherein the container is disposed above or below the coil unit. According to paragraph 1,
The sample heating and cooling device includes a plurality of containers, and the coil portion is formed as one to cover all of the plurality of containers. According to paragraph 1,
A sample heating and cooling device wherein the containers are formed in plural pieces, and the coil portions are formed in plural pieces to cover each of the plurality of containers. A container for receiving a sample;
a heating unit disposed to surround the outside of the container and receiving a heating fluid containing magnetic particles therein;
a coil part disposed to cover the heating part and generating a magnetic field to heat the sample to generate heat in the magnetic particles;
It includes a cooling unit that cools the sample,
The heating unit includes a first heating unit and a second heating unit arranged to be spaced apart along the longitudinal direction of the container,
A sample heating and cooling device wherein the first heating unit and the second heating unit include different magnetic particles so that the amount of heat generated under the influence of the magnetic field is different. In clause 7,
A sample heating and cooling device in which the magnetic particles generate heat through magnetic hysteresis loss caused by a magnetic field generated by the coil unit. In the sample heating and cooling method using a sample heating and cooling device including a sample, magnetic particles, a coil unit, a cooling unit, a power unit, a cooling unit, and a control unit,
Accommodating a sample containing magnetic particles in a container;
Applying power by the power supply unit to generate a magnetic field in the coil unit;
Heating the sample by causing the magnetic particles to generate heat by the magnetic field;
It includes: cooling the sample by a cooling unit,
A method for heating and cooling a sample, wherein the power supply unit is disposed externally and wirelessly transmits power to the sample heating and cooling device through a transmission coil, and the sample heating and cooling device receives power through the transmission coil. According to clause 9,
A method of heating and cooling a sample, wherein the step of heating the sample includes generating heat through magnetic hysteresis loss caused by the magnetic field generated by the coil unit.

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