KR102639927B1 - Dry tissue and separable wet wipes comprising the same - Google Patents

Abstract

The dry tissue according to an embodiment of the present invention is made by pulverizing a hot spring water composition containing hot spring water taken from 138-32 Seokjang-gil (137 Seokjang-ri), Gaegun-myeon, Yangpyeong-gun, Gyeonggi-do, Korea, and the powder is uniformly dispersed. The sulfur content of raw water is 3.99 mg/L.

Description

Dry tissues and separate wet tissues including same {DRY TISSUE AND SEPARABLE WET WIPES COMPRISING THE SAME}

Provides dry tissues and separate wet tissues containing them.

In order to increase the skin moisturizing or whitening effect, hot spring water is made by groundwater being heated above the average temperature by geothermal heat and seeping out to the surface. The water temperature is usually above 25℃, and it contains certain mineral components, water vapor, and gas. In the field of hot springs, hot springs, in a broad sense, refer to special natural water that comes to the surface from the ground and has physical and chemical properties that are different from ordinary water. In relation to this, springs that are lower than the water temperature of groundwater are called cold springs, and springs that contain a large amount of inorganic substances or gas components regardless of the water temperature are called mineral springs. The limiting temperature of hot springs varies depending on the region. In Korea, based on 25℃, those below 25℃ are classified as cold springs, 25-34℃ as mild springs, 34-42℃ as hot springs, and above 42℃ as hot springs. The classification of hot springs varies depending on scholars and countries, but is generally classified according to temperature, hot spring elution type, liquid ion concentration, mineral solubility, or hot spring development status.

Meanwhile, wet tissues are made by adding water to fabrics such as natural pulp or cotton and sterilizing them. They are used for skin cleansing and come in various forms such as oral wipes, baby wipes for cleaning, women's wipes, disposable wet towels, and multi-purpose wet wipes. It is produced with Because these wet tissues are easy to use and convenient, the demand for wet tissues is rapidly increasing.

Since wet tissues are used directly on human skin, they need to be excellent in safety to the skin. Most wet tissues use wet tissue packaging in the form of a packaging pack made of synthetic resin for packaging. In this case, the wet tissues are packaged in a state soaked in a solution, so even before opening, the wet tissues are used after manufacturing. Due to concerns about deterioration and decomposition, it is necessary to use a significant amount of preservative to have a preservative effect, which causes problems with human toxicity, and the period during which wet tissues are used without deterioration or decomposition after opening This can be shortened.

A separate wet tissue structure is being developed to solve these deterioration and corruption problems. Korean Patent No. 10-2025023 discloses a structure in which the solution is supplied from the solution storage unit to the tissue by the restoring force of the compressed tissue, and Korean Patent No. 10-1880046 discloses that when the water tube is broken, the liquid is supplied to the dry tissue. The applied structure is disclosed.

However, the dry tissue included in these conventional separate wet tissues is simply a carrier of liquid ingredients and is made of materials such as non-woven fabric and cotton, and the liquid ingredients include functional substances. Therefore, if the liquid component leaks or flows, functionality cannot be implemented and storage or carrying may be inconvenient.

A dry tissue and a separate wet tissue containing the same according to an embodiment of the present invention are intended to improve or prevent atopic dermatitis.

A dry tissue and a separate wet tissue containing the same according to an embodiment of the present invention are intended to improve dead skin cells, skin troubles, or skin tone.

A dry tissue and a separate wet tissue including the same according to an embodiment of the present invention are intended to improve user convenience.

Dry tissues and detachable wet tissues including them according to an embodiment of the present invention are intended to prevent deterioration or decay of the tissues.

Dry tissues and detachable wet tissues including them according to an embodiment of the present invention are intended to minimize hazards to the human body.

Dry tissues and detachable wet tissues containing the same according to an embodiment of the present invention are intended to extend the period of use.

In addition to the above tasks, embodiments according to the present invention can be used to achieve other tasks not specifically mentioned.

The dry tissue according to one embodiment of the present invention is made by powdering a hot spring water composition containing hot spring water taken from 138-32 Seokjang-gil (137 Seokjang-ri), Gaegun-myeon, Yangpyeong-gun, Gyeonggi-do, Korea, and the powder is uniformly dispersed.

Here, the sulfur content of the hot spring water is 3.99 mg/L.

The average diameter of the powder may be 0.05 to 50 ㎛.

The hot spring water composition is 0.43 mg/L of K, 143 mg/L of Na, 2.49 mg/L of Ca, less than 0.02 mg/L of Mg, 33.0 mg/L of SiO ₂ , 0.12 mg/L of Li, 0.20 mg/L. L of Sr, Fe less than 0.03 mg/L, Mn less than 0.01 mg/L, Cu less than 0.03 mg/L, Pb less than 0.03, Zn less than 0.05 mg/L, F ^- 19.9 mg/L, 119 mg /L of Cl ^- , and 9.97 mg/L of SO ₄ ^{2 -} .

A detachable wet tissue according to an embodiment of the present invention includes the dry tissue.

Separate wet tissues may be for improving or preventing atopic dermatitis, for improving skin, or for cosmetic use.

Dry tissues and detachable wet tissues containing the same according to an embodiment of the present invention can improve or prevent atopic dermatitis, improve skin keratin, skin troubles or skin tone, and improve user convenience. It can prevent tissue deterioration or decay, minimize harmfulness to the human body, and extend the period of use.

Figure 1 is a test report of the hot spring water of the present invention.
Figure 2 is a diagram showing the effect of the hot spring water of the present invention on skin keratin. In the figure, the lower the keratin analysis value, the better the skin keratin is, so the rate of change expressed as a negative value can be viewed as the degree of skin keratin improvement.
Figure 3 is a diagram showing the effect of the hot spring water of the present invention on skin tone.
Figure 4 is a diagram showing the effect of the hot spring water of the present invention on skin moisturizing power.
Figure 5 is a diagram showing the experimental process of the present invention.
Figure 6A is a graph showing body weight changes in experimental mice, and Figure 6B is a graph showing clinical skin characteristics and severity of skin lesions in atopic dermatitis NC/Nga mice induced by DNCB.
Figures 7a to 7e are photographs showing clinical evaluation results in NC/Nga mice with atopic dermatitis induced by DNCB.
Figure 8 is a graph showing the total number of cells in the back skin and ALAN in NC/Nga mouse.
Figure 9 is a graph showing blood characteristics in NC/Nga mouse.
Figure 10 is a graph showing serum IgE levels in NC/Nga mice with atopic dermatitis induced by DNCB.
Figure 11 shows the results of measuring cytokine production (IL-4, IL-5, IL-13, IL-10, and IFN-γ) in the spleen cell culture supernatant 5 weeks after application of DNCB.
Figure 12 shows the expression of inflammatory cytokines (IL-13, IL-6, TNF-α) and itching-related genes (il-31R, CCR-3) in back skin tissue 5 weeks after DNCB application by real-time PCR. Shows the analysis results.
Figure 13 shows the results of group analysis of several immune cell subtypes in PBMC in a DNCB-induced atopic dermatitis murine model (n=6).
Figures 14 to 19 are pictures showing PBMC content percentage by FACS analysis.
Figure 20 is a photograph showing the results of H&E staining of skin tissue.
Figure 21 is a photograph showing the results of Toluidine Blue staining of skin tissue.

With reference to the attached drawings, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. The present invention may be implemented in many different forms and is not limited to the embodiments described herein. In order to clearly explain the present invention in the drawings, parts not related to the description are omitted, and the same reference numerals are used for identical or similar components throughout the specification. Additionally, in the case of well-known and well-known technologies, detailed descriptions thereof are omitted.

Throughout the specification, when a part is said to “include” a certain element, this means that it may further include other elements rather than excluding other elements, unless specifically stated to the contrary.

Throughout the specification, “composition” refers to a material in which two or more components are uniformly mixed, and is a concept that includes not only the finished sample but also intermediate materials for manufacturing the finished sample.

In the dry tissue according to the example, powder manufactured by powdering a hot spring water composition containing hot spring water taken from 138-32 Seokjang-gil (137 Seokjang-ri), Gaegun-myeon, Yangpyeong-gun, Gyeonggi-do, Korea, is uniformly dispersed. Here, the amount of sulfur content in the raw hot spring water is 3.99 mg/L.

Here, the hot spring water composition is 0.43 mg/L of K, 143 mg/L of Na, 2.49 mg/L of Ca, less than 0.02 mg/L of Mg, 33.0 mg/L of SiO ₂ , 0.12 mg/L of Li, 0.20 mg/L of Mg. mg/L Sr, less than 0.03 mg/L Fe, less than 0.01 mg/L Mn, less than 0.03 mg/L Cu, less than 0.03 mg/L Pb, less than 0.05 mg/L Zn, 19.9 mg/L F ^- , It may contain 119 mg/L of Cl ^- , and 9.97 mg/L of SO ₄ ^{2 -} .

A hot spring water composition having this composition and content can improve or prevent atopic dermatitis, and improve skin exfoliation, skin troubles, or skin tone. Additionally, the hot spring water composition can be used for cosmetic or bathing purposes. Additionally, the hot spring water composition can also be used as dry tissue or wet tissue for infants.

The results of a human application test conducted on the hot spring water powdered and applied to the dry tissue according to the present invention at the KC Skin Clinical Research Center are summarized as follows.

An evaluation was conducted to evaluate the efficacy of the hot spring water of the present invention on improving skin keratin, skin tone, and skin moisturizing power. The test was conducted on 21 adult women. A total of 21 test subjects applied the sample to the entire face once and twice. As a result of using the sample for 2 weeks, 1) As a result of measuring skin keratin after using the sample, the skin keratin analysis value decreased statistically significantly both immediately after using the sample and 2 weeks after using the sample compared to before using the sample (p<0.05), 2) After using the sample As a result of measuring skin tone after using the sample, the skin tone analysis value increased statistically significantly both immediately after using the sample and 2 weeks after using the sample compared to before using the sample (p<0.05). 3) As a result of measuring skin moisturization after using the sample, skin moisture content The analysis value increased statistically significantly both immediately after sample use and 2 weeks after sample use compared to before sample use (p<0.05).

In addition, the hot spring water of the present invention was commissioned by Daejeon University and tested on atopic dermatitis-induced mice. The results of the atopy-related experiment can be briefly summarized as follows.

1) In the IFK506 application group and bathing group, compared to the control group (DNCB_CTL), the positive control group (FK506) was 52.6% (p<0.001), and the spring water bathing group was 40.5% (p<0.001). Clinical evaluation (skinclinical score) values decreased significantly.

2) Among the tap water bathing group and the spring water bathing group, only the spring water bathing group had Neutrophils + cells, CD4 + Th cells, CD8 + c/sT cells, and B220 + cells in PBMC and back skin tissue, respectively. The total absolute cell number of CD11b+ cells decreased compared to the control group.

3) Among the tap water bathing group and the spring water bathing group, only the spring water bathing group significantly decreased the serum IgE level compared to the control group.

4) After stimulation of spleen cells with anti-CD3 in the spring water bathing group, the production of IL-5 and IL-13 in the culture supernatant was significantly decreased compared to the control group.

5) In the spring water bathing group, the total number of back skin cells significantly decreased compared to the control group.

6) The frequency (%) and absolute cell number of inflammatory immune cells (Gr-1+CD11b+) in ALN (CD4+) and back skin tissue decreased in the spring water bathing group compared to the control group.

7) Compared to the control group, the expression of the Th2 cytokines il-4, IL-5, and IL-13 mRNA and the IL-31 mRNA gene, which has the causative mechanism of itching, was statistically significantly higher in the back skin tissue of the spring water bathing group. was significantly suppressed.

8) In the spring water bathing group, the thickness of the epidermis and the infiltrated inflammatory immune cells and mast cells in the dermis area were significantly reduced in the back skin tissue.

The hot spring water composition can be powdered and applied to dry tissue.

Any powdering process can be applied as long as it can produce the hot spring water composition into powder with the required average diameter. For example, the hot spring water composition can be heated, dried, and then micronized to produce powder.

The average diameter of the powder may be 0.05 to 50 ㎛. Within this range, when the powder is applied to dry tissue, it can deliver an optimal effect on the skin. Compared to the case where the hot spring water composition is applied to the skin in liquid form, in the case of the powder form having the above-mentioned average diameter, the mineral components contained in the hot spring water can be more effectively delivered to the skin, thereby improving the performance of atopic dermatitis and skin improvement. It can be.

The powders may have the same average diameter and be applied to dry tissue, or two or more types of powders having different average diameters may be applied.

Additionally, if necessary, in addition to the hot spring water composition powder according to the embodiment, other powders having additional functionality may be further included.

The process of dispersing the powder into dry tissue may be a known process. For example, the powder may be spray-applied in an aerosol state and then dried, electrostatically applied and then applied by compression using a roller, or a suspension may be prepared and dried after being immersed in a dry tissue. Alternatively, hot spring water can be stirred into oil containing an emulsifier, subjected to micronization and homogenization, and then moisture is removed to create a form in which the emulsifier coats the mineral components, thereby protecting the functional ingredients from external factors.

In addition, in order to uniformly disperse the hot spring water powder on the dry tissue, vibration may be generated using an ultrasonic generator or a vibrator.

The separate wet tissue according to the embodiment includes a dry tissue in which the powder of the above-described hot spring water composition is dispersed.

In the dry tissue according to the embodiment, the functional ingredients are already applied in powder form, so the user can use the dry tissue by moistening it with water (H ₂ O) without separately preparing a liquid containing the functional ingredients. Convenience can be greatly increased.

In the case of applying the hot spring water composition in liquid form to a general dry tissue, it is inconvenient to carry, and if a defect occurs in the package, etc., the liquid component may leak and damage may occur. If the liquid component leaks, the hot spring water component may cause damage. Various effects cannot occur. On the other hand, in the case of the dry tissue according to the embodiment, it is very easy to carry, there is no need to carry water separately, and the user can easily obtain water nearby and apply it to the dry tissue, so convenience can be greatly improved. .

In addition, the dry tissue according to the example can be stored stably for a long time compared to the liquid hot spring water composition.

The dry tissues to which the powder is applied can be packaged separately from water (H ₂ O), and when the dry tissues are withdrawn from these separate wet tissues, the package in which the water is stored ruptures and the water moves to the dry tissues. May be included. Additionally, the detachable wet tissue may be provided with a dispenser of a specific structure so that water can be effectively and uniformly applied to the dry tissue.

Below, the present invention will be described in more detail through examples and experimental examples. However, the present invention is not limited to the following examples.

Example 1: Preparation of hot spring water

To collect hot spring water from Gaegun-myeon, Yangpyeong-gun, hot spring water was collected from 138-32 Seokjang-gil (137 Seokjang-ri), Gaegun-myeon, Yangpyeong-gun, Gyeonggi-do, the source of the water. The coordinates of the borehole were WGS84, 37°27'14.16"N, 127°30'37.36"E, and 35m altitude.

In addition, as a result of on-site sulfur content (Total sulfides; H2S, HS-) analysis using HACHDR/2800, the amount of sulfur content in the raw water was 3.99 mg/L.

In addition, we commissioned the Korea Institute of Geoscience and Mineral Resources to conduct an experiment on the amount of sulfur content according to the conditions as follows.

go. Experimental conditions

1) Experimental condition 1, time elapsed condition

- Groundwater samples were sealed in 500mL containers and stored at room temperature (22.9℃ ~ 25.3℃), then each 500mL container was opened at one-week intervals to analyze the amount of sulfur content in the groundwater and observe the change in sulfur content over time.

2) Experimental condition 2, storage container size conditions

- Groundwater samples were sealed in a 10L container and stored at room temperature. After 5 weeks, the sulfur content was analyzed and compared with the sulfur content in a small 500mL container.

3) Experimental condition 3, temperature conditions during storage

- Groundwater samples were sealed in a 500 mL container and stored in refrigeration (40℃), and the sulfur content was analyzed for 5 weeks and compared with the sulfur content of samples stored at room temperature (22.9℃ ~ 25.3℃).

me. Experiment result

1) Experimental condition 1, time elapsed condition

- The amount of sulfur content measured on site was 3.99 mg/L, and the amount of sulfur content measured indoors every week decreased rapidly, showing 0.091 mg/L after 2 weeks.

- Due to the sealed environment of each sample at the site and the heterogeneity of the sulfur content in groundwater, the amount of sulfur content increases and decreases after 2 weeks.

2) Experimental condition 2, storage container size conditions

- As a result of analyzing the amount of sulfur content in the 10L container after 5 weeks, it was 0.067mg/L, which was lower than the amount of sulfur content in the 500mL container of 0.129mg/L.

3) Experimental condition 3, temperature conditions during storage

- As a result of analyzing the sulfur content of groundwater in a 500 mL container refrigerated (4.0℃) for 5 weeks, it was found to be 2.50 mg/L, which is 19 times more than the 0.129 mg/L of the sample stored at room temperature.

The Korea Institute of Geoscience and Mineral Resources (124 Gwahak-ro, Yuseong-gu, Daejeon) was requested to report the test results of hot spring water taken from 138-32 Seokjang-gil (137 Seokjang-ri), Gaegun-myeon, Yangpyeong-gun, Gyeonggi-do, Korea, and the test results of the hot spring water are shown in Figures 1a and 1. Shown in 1b.

As shown in Figure 1, as a result of analyzing the components of hot spring water, the hot spring water of the present invention has 0.43 mg/L of K, 143 mg/L of Na, 2.49 mg/L of Ca, less than 0.02 mg/L of Mg, and 33.0 mg. /L SiO ₂ , 0.12 mg/L Li, 0.20 mg/L Sr, less than 0.03 mg/L Fe, less than 0.01 mg/L Mn, less than 0.03 mg/L Cu, less than 0.03 Pb, 0.05 It contained minerals of mg/L of Zn, 19.9 mg/L of F ^- , 119 mg/L of Cl ^- , and 9.97 mg/L of SO ₄ ^2- .

Experimental Example 1: Evaluation of the efficacy of the hot spring water of the present invention on improving skin exfoliation, skin tone, and skin moisturizing power

1) Test personnel

- A total of 21 test subjects who fit the selection criteria and did not meet the exclusion criteria (average age: 49.333±5.944 years)

2) Test method:

- In order to confirm the effectiveness of the sample in improving skin exfoliation, skin tone, and skin moisturization, a test site was selected for a total of 21 test subjects who met the selection criteria and did not meet the exclusion criteria among those who agreed to participate in this human application test. After using the sample once and for 2 weeks, photos were taken and skin measurements were taken before and after using the sample.

3) Evaluation method

- Skin keratin measurement: Visioscan VC98

- Skin tone measurement: Janus 1 Mark II

- Measurement of skin moisturizing power: Epsilon

4) Safety evaluation results:

During the period of use of this sample, no special skin adverse reactions were reported on the area where the sample was used.

5) Summary of experiment results:

This test was conducted on 21 adult women aged 19 to 60, and a total of 21 test subjects used the hot spring water of the present invention on their entire face once and for 2 weeks.

1) As a result of measuring skin keratin after using the sample, the skin keratin analysis value decreased statistically significantly both immediately after using the sample and 2 weeks after using the sample compared to before using the sample (p<0.05).

2) As a result of measuring skin tone after using the sample, the skin tone analysis value increased statistically significantly both immediately after using the sample and 2 weeks after using the sample compared to before using the sample (p<0.05).

3) As a result of measuring skin moisturization after using the sample, the skin moisture content analysis value increased statistically significantly both immediately after using the sample and 2 weeks after using the sample compared to before using the sample (p<0.05).

Therefore, it is judged that the hot spring water, which is a sample according to the example, helps improve skin exfoliation, skin tone, and skin moisturization when used once and for two weeks. Sample information is listed in Table 1 below.

All ingredients in the sample spa Sample properties Colorless transparent liquid Test area full face How to use After putting the sample in a clean container, gently roll it and wipe it as if it is dissolving sebum and dead skin cells all over your face. After the final rinse, rather than wiping off the moisture with a towel, pat it with your hand to allow it to absorb into the skin. Period of use 1 time and 2 weeks (2 times a day)

Test Methods

1. Test subject visit and progress schedule

1) Visit 1

- Confirmation of test subject consent and test subject selection/exclusion criteria

- Take photos and measure skin before using samples

- Use samples

- Take photos and measure skin immediately after using the sample

- Confirmation of adverse reactions and questionnaire evaluation of test subjects

- Issuance of samples and compliance log

2) Visit 2

- Photo taking and skin measurement 2 weeks after using the sample

- Confirmation of adverse reactions and questionnaire evaluation of test subjects

- Collection of compliance logs

2. Skin measurement method and procedure

For instrumental skin measurement, test subjects washed their faces with a prepared facial cleanser and then allowed their skin to settle for about 30 minutes in a room with constant temperature and humidity conditions (temperature 20-24°C, humidity 40-60%) to adapt to the measurement environment. The sample application area was measured and conducted by the same researcher under the same conditions each time.

3. Skin efficacy evaluation items

1) Measurement of skin keratin

- For skin keratin measurement, corneofix, a special adhesive tape, was used to collect keratin from the cheek area of the face before using the sample, immediately after using the sample, and 2 weeks after using the sample, and photographed with a Visioscan VC98. The average desquamation index (DI) of the taken photos was used as evaluation data, and the unit of the measured desquamation value is %. Since the analysis value and the degree of skin keratin improvement are inversely proportional, a lower analysis value means that skin keratin is improved.

2) Skin tone measurement

- Skin tone was measured using Janus 1 Mark II, which can take pictures of the entire face, before using the sample, immediately after using the sample, and two weeks after using the sample. Among the photos taken, a specific area of the normal light mode image was designated and the level of skin tone was analyzed and used as evaluation data. The unit of analyzed skin tone is %, and the analysis value and the degree of skin tone improvement are proportional.

3) Measurement of skin moisturizing power

- To measure skin moisturizing power, the cheek area of the face was measured before using the sample, immediately after using the sample, and 2 weeks after using the sample, using Epsilon, which can take images of the skin moisture content. The level of skin moisture in a single image was analyzed and used as evaluation data, and the unit is AU (Arbitrary Unit). The analysis value and the degree of improvement in skin moisturization are proportional.

4. Evaluation of adverse skin reactions

- In order to do our best to ensure the safety of test subjects, a survey and visual evaluation of adverse reactions were conducted at each visit and the occurrence and severity of symptoms were confirmed. Test personnel instructed test subjects to report immediately if they felt any adverse reactions while using the sample. If an adverse reaction or adverse event occurs, the person in charge of the study immediately reports it to the principal investigator, and the principal investigator determines appropriate action accordingly and whether the subject should participate in the study.

5. Assessing compliance with sample use

- Test subjects were instructed to write in the compliance log distributed with the sample whether or not to use the sample when using the sample according to the specified usage and number of uses. Test subjects who used less than 80% of the total sample compliance of 100% were excluded from the measurement data analysis values.

6 Subject survey evaluation

- After using the sample, the test subject completed a direct questionnaire evaluation on the skin improvement efficacy and preference of the sample on a 5-point scale (5: very satisfied, 4: satisfied, 3: average, 2: dissatisfied, 1: very dissatisfied). The number of test subjects who responded with 3 or more points was expressed as a percentage (positive response rate).

7. Statistical result analysis method

- To determine the significance of the change in skin measurements before and after using the sample, it was verified using SPSS 230, a statistical analysis program. Statistical significance was confirmed when the probability of significance was p<005 in the 95% confidence interval.

1) Comparison before and after sample use (single measurement)

- Satisfies normality test: Paired samples T-test (parametric method)

- Unsatisfactory normality test: Wilcoxon signed ranks test (non-parametric method)

8. Skin measurement results

1) Skin keratin measurement results

- As a result of measuring skin keratin after using the sample, the skin keratin analysis value decreased statistically significantly both immediately after using the sample and 2 weeks after using the sample compared to before using the sample (p<0.05), and the results are shown in Table 2 below.

Skin keratin changes hour Mean±standard deviation (%) Rate of changea(%) Significance probability b (p value) Before using samples 5.165 ± 1.099 - - Immediately after using the sample 4.364 ± 1.101 -14.934 0.000* 2 weeks after sample use 4.407 ± 1.002 -12.270 0.006* * Rate of change a (%) = [(analysis value after sample use - analysis value before sample use) / analysis value before sample use] x 100
* Significance probability b (p value) *: p<0.05 by Paired samples T-test

2) Skin tone measurement results

- As a result of measuring skin tone after using the sample, the skin tone analysis value increased statistically significantly both immediately after using the sample and 2 weeks after using the sample compared to before using the sample (p<0.05), and the results are shown in Table 3 below.

skin tone changes hour Mean±standard deviation (%) Rate of changea(%) Significance probability b (p value) Before using samples 56.857 ± 2.265 - - Immediately after using the sample 57.048 ± 2.312 0.335 0.042* 2 weeks after sample use 57.190 ± 2.205 0.599 0.049* * Rate of change a (%) = [(analysis value after sample use - analysis value before sample use) / analysis value before sample use] x 100
* Significance probability b (p value) *: p<0.05 by Paired samples T-test

3) Skin moisturizing power measurement results

- As a result of measuring skin moisturization after using the sample, the skin moisture content analysis value increased statistically significantly both immediately after using the sample and 2 weeks after using the sample compared to before using the sample (p<0.05), and the results are shown in Table 4 below. .

Changes in skin moisture content hour Mean±standard deviation (AU) Rate of changea(%) Significance probability b (p value) Before using samples 21875±5113 - - Immediately after using the sample 23743±5412 8742 0.000* 2 weeks after sample use 23786±5009 9508 0.000* * Rate of change a (%) = [(analysis value after sample use - analysis value before sample use) / analysis value before sample use] x 100
* Significance probability b (p value) *: p<0.05 by Paired samples T-test

Experimental Example 2: Study on anti-atopy effect of tap water/hot spring water

NC/Nga mice with hair removal and atopic dermatitis induced by DNCB were bathed and swam for 20 minutes every day for 3 weeks after DNCB induction. At this time, the water temperature was 28 ℃ on average between 25 ℃ and 30 ℃.

1. Body weight and skin clinical scores of atopic dermatitis NC/Nga mice

As can be seen in Figure 6A, there was no change in body weight in all experimental groups.

Figure 6B shows that compared to the normal group, the clinical evaluation (skin clinical score) 1 to 2 weeks after DNCB application showed a more than 3-fold increase in erythema, papules, scales and crusts accompanied by itching on the back skin area, and DNCB application. After 5 weeks, the skin clinical score of the control group (DNCB_CTL) showed a statistically significant increase of more than 2 times compared to the normal group (naive NC/Nga mice) (p<0.001). And 5 weeks after DNCB application, that is, in the FK506 application group and bathing group for 3 weeks, compared to the control group (DNCB_CTL), the positive control group (FK506) decreased by 52.6% (p<0.001) and the spring water bathing group decreased by 40.5% (p<0.001). ), the clinical evaluation (skin clinical score) value decreased significantly.

2. Atopic skin rash clinical evaluation (skin clinical score)

After 7-week-old NC/Nga mice were acclimatized in the laboratory for 1 week, the entire back of the mouse (from the lower part of the ear to the upper part of the tail) was removed, left for 24 hours, and then treated with 1% DNCB (1-chloro-2, 4-dinitro benzene). ) containing acetone: 200 ㎕ of olive oil (3:1) was applied to the hair removal area and applied a second time 3 days later. From 10 weeks of age, atopic dermatitis was induced by reapplying 150 ㎕ of 0.4% DNCB solution 3 times a week for 3 weeks (10 to 13 weeks of age). Visual evaluation of skin rash was performed at 10 weeks of age (DNCB) by referring to Yamamoto's evaluation items. Evaluation was conducted at 1 week of application), 12 weeks of age (2 weeks of DNCB application), and 15 weeks of age (5 weeks of DNCB application). The visual evaluation items are five items: erythema/hemorrhage with itching behavior, dryness/scarring, edema, excoriation/erosion, and lichenification. The visual evaluation results were expressed as the total of each evaluated score. Each item was scored as none (0), mild (1), moderate (2), and severe (3), and the score was measured from a minimum of 0 to a maximum of 15 (Figure (see 7a~7e)

3. Total cell count

Five weeks after application of DNCB, the total number of back skin tissue cells (Figure 8A) and the number of axillary lymph node cells (see Figure 8B) significantly increased in the control group (DNCB_CTL) compared to the normal group (naive NC/Ng amice). (p<0.001, p<0.01). And 5 weeks after DNCB application, that is, for 3 weeks, there was a statistically significant decrease in the positive control group (FK506) and spring water bathing group compared to the control group (DNCB_CTL) in the FK506 application group and bathing group. In addition, among the tap water bathing group and the spring water bathing group, only the spring water bathing group was found to be effective in suppressing the total back skin tissue cell count and the axillary lymph node cell count.

4. Blood hematology: wthite blood cells (WBC) level

Five weeks after DNCB application, the total white blood cell count (Figure 9A) and lymphocyte frequency (Figure 9B, %) were significantly decreased in the control group (DNCB_CTL) compared to the normal group (naive NC/Nga mice) (p< 0.001). And 5 weeks after DNCB application, that is, for 3 weeks, there was a statistically significant decrease in the spring water bathing group compared to the control group (DNCB_CTL) in the FK506 application group and the bathing group (p<0.001). In addition, among the tap water bathing group and the spring water bathing group, only the spring water bathing group showed an effect of increasing the total white blood cell count and lymphocyte frequency.

5. Measurement of serum IgE levels

After 7-week-old NC/Nga mice were acclimatized in the laboratory for 1 week, the entire back of the mouse (from the lower part of the ear to the upper part of the tail) was removed, left for 24 hours, and then treated with 1% DNCB (1-chloro-2, 4-dinitro benzene). ) containing acetone: 200 ㎕ of olive oil (3:1) was applied to the hair removal area and applied a second time 3 days later. From 10 weeks of age, atopic dermatitis was induced by re-applying 150 ㎕ of 0.4% DNCB solution 3 times a week for 3 weeks (10 to 13 weeks of age). Serum IgE levels were measured at 10 weeks of age (1 week of DNCB application) and 12 weeks of age. At 1 week of age (2 weeks after application of DNCB) and 15 weeks of age (5 weeks after application of DNCB), approximately 100 ㎕ of blood was collected from the retro-orbital venusplexus using a capillery tube and centrifuged at 6,500 rpm for 20 minutes. After that, 30 μl of serum was separated. Serum was taken and stored frozen at -70°C, and ELISA method was used to measure IgE concentration in the serum of NC/Nga mice. Mix 5 ㎕ (1/10 dilution) of serum collected from NC/Nga mice and 45 ㎕ of dilution buffer and dispense into each well. Leave at room temperature at 25°C for 2 hours and wash twice with washing buffer. Then, biotin-conjugated anti-IgE was added and left for 2 hours. After washing again with water twice, it was washed with a buffer solution, treated with 100 ㎕ of Avidin-HRP conjugated antibody, left at room temperature for 1 hour, and then washed again. In addition, 100 ㎕ of TMB substrate was dispensed, left in the dark for 30 minutes, treated with 100 ㎕ of stop solution, and the absorbance was measured at 450 nm with an ELISA reader.

The serum IgE level 5 weeks after DNCB application (Figure 10) showed a significant increase in the control group (DNCB_CTL) compared to the normal group (naive NC/Nga mice) (p<0.001), 5 weeks after DNCB application, i.e. , There was a statistically significant decrease in the FK506 application group and the spring water bathing group compared to the control group (DNCB_CTL) in the FK506 application group and bathing group for 3 weeks (p<0.01). In addition, it was found that among the tap water bathing group and the spring water bathing group, only the spring water bathing group showed the effect of reducing serum IgE levels.

6. Results of measuring cytokine production (IL-4, IL-5, IL-13, IL-10, IFN-γ) in spleen cell culture supernatant 5 weeks after DNCB application

As can be seen in Figure 11, IL-4 production, IL-5 production, IL-13 production, and IFN-γ production were significantly increased in the control group (DNCB_CTL) compared to the normal group (naive NC/Nga mice). (p<0.001, p<0.01) And 5 weeks after DNCB application, that is, for 3 weeks, the FK506 application group and the spring water bathing group showed statistically significant results compared to the control group (DNCB_CTL) in the FK506 application group and the bathing group. decreased (p<0.001, p<0.01). In addition, among the tap water and spring water bathing groups, only the spring water bathing group showed IL-4 production, IL-5 production, and IL-5 production in the spleen cell culture supernatant. It was found that IL-13 production and IFN-γ production decreased.

7. Inflammatory cytokines (IL-13, IL-6, TNF-α) and pruritus-related gene expression (il-31R, CCR-3) in back skin tissue 5 weeks after DNCB application were analyzed by real-time PCR. one result

As can be seen in Figure 12, IL-31R mRNA gene expression, IL-13 mRNA gene expression, IL-6 mRNA gene expression, and CCR3 mRNA gene expression were significantly higher in the control group (DNCB_CTL) than in the normal group (naive NC/Nga mice). This significantly increased mRNA gene expression (p<0.001, p<0.01, p<0.05), and 5 weeks after DNCB application, that is, for 3 weeks, the FK506 application group and the FK506 application group and the bathing group compared to the control group (DNCB_CTL) There was a statistically significant decrease in the spring water bathing group (p<0.01, p<0.05). In addition, among the tap water bathing group and the spring water bathing group, only the spring water bathing group showed decreased IL-31R mRNA gene expression, IL-13 mRNA gene expression, IL-6 mRNA gene expression, and CCR3 mRNA gene expression. It was found that it was effective.

8. Group analysis of multiple immune cell subtypes in PBMCs in DNCB-induced atopic dermatitis murine model (n=6)

In Figures 13A and 13B, the change in immune lymphocyte cell frequency (%) in PBMC 5 weeks after DNCB application shows that the lymphocyte cell frequency (lymphocytes) and CD3+ T & CD19+ B cell frequency (%) are compared to normal group (naive NC/Nga mice). ), the control group (DNCB_CTL) showed a statistically significant decrease (p<0.001) (see Figures 14 and 15).

And 5 weeks after DNCB application, that is, for 3 weeks, there was a statistically significant increase in the FK506 application group and the spring water bathing group compared to the control group (DNCB_CTL) in the FK506 application group and the bathing group (p<0.001, p<0.001). In addition, among the tap water bathing group and the spring water bathing group, only the spring water bathing group showed a reduction effect in lymphocyte cell frequency and CD3+ T cell frequency (%).

13C to 13F, changes in immune lymphocyte cell frequency (%) in PBMC 5 weeks after DNCB application, CD4+ Th & CD8+c/s T cell frequency (%), CD4+/CD69+ activated T cell frequency (%), CD23+/B220+ activated B cell frequency (%) and Gr-1+/CD11b+ cell frequency (%) showed a statistically significant increase in the control group (DNCB_CTL) compared to the normal group (naive NC/Nga mice) ( p<0.001,p<0.05) (see FIGS. 16 to 19). And 5 weeks after DNCB application, that is, for 3 weeks, the FK506 application group and the spring water bathing group decreased compared to the control group (DNCB_CTL) in the FK506 application group and the bathing group. In addition, among the tap water bathing group and the spring water bathing group, only the spring water bathing group showed CD4+Th & CD8+c/s T cell frequency (%), CD4+/CD69+ activated T cell frequency, and Gr- It was found that the 1+/CD11b+ cell frequency (%) showed a reduction effect.

9. Skin tissue staining

In Figures 20 and 21, compared to the normal group, epidermis thickness was hyperplastic and expanded in skin tissue such as mice 5 weeks after DNCB application (green arrow), and pigmentation of surrounding tissue cells, parakeratosis, infiltration of mast cells, and granule production were observed. showed an increase. And 5 weeks after DNCB application, that is, for 3 weeks, the thickness of the epidermis in the skin tissue of the FK506 application group and the spring water bathing group was hyperplastic and expanded compared to the control group (DNCB_CTL) in the FK506 application group and the bathing group (green arrow). Pigmentation of surrounding tissue cells, parakeratosis, mast cell infiltration, and granule production were reduced. In addition, among the tap water bathing group and the spring water bathing group, only the spring water bathing group showed hyperplasia and expansion of epidermis thickness in skin tissue (green arrow), pigmentation of surrounding tissue cells, parakeratosis, and mast cells. It was found that the infiltration, granule production, etc. showed a reduction effect.

The hot spring water of the present invention was commissioned by the Korea Chemical Convergence Research Institute and a human patch test was conducted on a total of 33 test subjects for safety evaluation over a period of 4 days. As a result, the skin reactivity of the natural sulfur hot spring water was 0.03. It was judged to be non-irritating according to the criteria for judging 0.00 to 0.75 as non-irritating.

In addition, the hot spring water of the present invention was applied to the skin and was judged to be a sample that could help improve skin exfoliation, skin tone, and skin moisturization by using it once and for 2 weeks. As can be seen from the skin tissue examination and serum IgE level, tap water ( In the Tap_water) bathing group and the Spring_water bathing group, only the Spring water bathing group showed atopic dermatitis by expanding epidermis thickness due to hyperplasia, reducing infiltration of mast cells, and reducing blood IgE levels in the DNCB-induced atopic skin rash model. It has been proven to be effective in treating/improving sexual skin rashes.

Experimental Example 3: Evaluation of efficacy and safety of tap water/hot spring water in improving itching sensation, etc.

1) Test personnel

- A total of 30 test subjects (adult men and women between the ages of 19 and 64) who fit the selection criteria and do not meet the exclusion criteria.

- 15 people in the test group (sulfur spring water group), 15 people in the control group (Arisu group)

2) Test method:

- Among those who agreed to participate in this human application test, a total of 30 test subjects (3 visits) who met the selection criteria and did not meet the exclusion criteria were treated with previously used products (face wash or body cleanser) for 4 weeks. After washing your face or taking a shower, open the test sample (sulfur spring water) or control sample (Arisu), rinse the area with itching (Visual Analogue Scale) with 250 ml, and then rinse again with 250 ml of the remaining test sample or control sample. After rinsing the itchy area once, the amount of change in transepidermal moisture loss, skin roughness, erythema, and dead skin cells is measured according to the change in itching sensation.

3) Evaluation method

- Transepidermal water loss: Tewameter TM300

- Skin moisture content: corneometer CM825

- Skin roughness and dead skin cells: VisioScan VC20 PLUS

- Skin erythema measurement: Mexameter MX18

- Evaluation of the degree of itching (Visual Analogue Scale, VAS) (see Table 5 below)

In this specification, itching is a sensation that causes the urge to scratch. Although the nature and physiological mechanism of itching are not yet well known, peripheral chemical mediators released by a wide range of skin stimuli or harmful substances act on peripheral nerve fibers. It is triggered by doing. Additionally, itching may be caused by dry skin. It gets worse in the seasonally dry winter, and scratching the skin due to itching can lead to eczema and infection. Symptoms may worsen due to lack of moisture and sebum.

4) Safety evaluation results:

During the period of use of this sample, no special skin adverse reactions were reported on the area where the sample was used.

Test Methods

1. Test subject visit and progress schedule

The details of the visit and examination are shown in Table 6 below.

i) The margin of error for each visit is set to 4 days.

ii) Consent form is implemented prior to all human application test-related activities.

iii) Demographic survey: date of birth, gender, height, weight

iv) Pregnancy test (performed only for screening)

2. Skin measurement method and procedure

To accurately measure skin condition, an instrumental evaluation is conducted to check skin barrier biophysical indicators in a skin diagnosis room equipped with constant temperature and humidity facilities. Constant temperature and humidity conditions are set to an indoor temperature of approximately 20 to 24 ℃ and an indoor humidity of approximately 40 to 60%. The subject rests in the skin examination room for 30 minutes to allow the temperature and humidity of the skin surface to adapt to the temperature and humidity of the measurement space, and limits water intake for accurate evaluation. The evaluation data of transepidermal water loss, skin water content, erythema measurement, roughness, and keratin measurement are measured in the area of the body with itching sensation. Skin measurement allows one examiner to measure the same area by marking the location for objective results.

3. Evaluation of adverse skin reactions

- In order to do our best to ensure the safety of test subjects, a survey and visual evaluation of adverse reactions were conducted at each visit and the occurrence and severity of symptoms were confirmed. Test personnel instructed test subjects to report immediately if they felt any adverse reactions while using the sample. If an adverse reaction or adverse event occurs, the person in charge of the study immediately reports it to the principal investigator, and the principal investigator determines appropriate action accordingly and whether the subject should participate in the study.

4. Assessing compliance with sample use

- Test subjects were instructed to write in the compliance log distributed with the sample whether or not to use the sample when using the sample according to the specified usage and number of uses. Test subjects who used less than 75% of the total sample compliance of 100% were excluded from the measurement data analysis values.

5. Effectiveness evaluation

1) Primary efficacy evaluation variable

- Change in itching (VAS) from visit1 to visit3

2) Secondary efficacy evaluation variables

- Change in itching (VAS) from visit1 to visit2

- Changes in skin moisture content from visit1 to visit2 and from visit1 to visit3 measured with a corneometer

- Changes in transepidermal water loss (Tewameter) from visit1 to visit2 and from visit1 to visit3

- Changes in skin roughness (VisioScan VC20 PLUS) from visit1 to visit2 and from visit1 to visit3

- Changes from visit1 to visit2 and from visit1 to visit3 in erythema (Mexameter MX18)

- Changes from visit1 to visit2 and from visit1 to visit3 in dead skin cells (VisioScan V20 PLUS)

3) Safety evaluation variables

- Adverse reactions reported by the subject or observed by an oriental medicine doctor

6. Evaluation criteria

The analysis target group for this human application test is divided into the modified ITT (intent-to-treat) analysis target group, PP (per-protocal) analysis target group, and safety analysis target group.

The primary efficacy evaluation variable is analyzed using both the Modified ITT and PP analysis target groups, but the effect of the primary efficacy evaluation variable is determined using the Modified ITT analysis target group.

The secondary efficacy evaluation variables are analyzed using the Modified ITT and PP analysis target groups, but the results of the secondary effectiveness evaluation variables are determined using the Modified ITT analysis target group. Safety evaluation uses the safety analysis target group.

The definition of the analysis group is as follows.

1) Modified ITT analysis target group

- An analysis group consisting of clinical trial subjects who can obtain data on efficacy evaluation variables after using samples for human application testing at least once

2) PP analysis target group

- Among the subjects included in the Modified ITT analysis target group, clinical trial subjects who completed the human application test and performed the entire human application test process without violating the provisions of the human application test protocol after starting the clinical trial as specified in the protocol analysis group

3) Safety analysis target group

- Analysis group obtained from all clinical trial subjects that used samples for human application testing at least once and for which safety evaluation was conducted

7. Statistical analysis and evaluation of efficacy evaluation variables

1) Analysis of primary efficacy evaluation variables

The primary efficacy evaluation variable is to compare the change in the test subject's itching sensation measured in visit1 and visit3 by the test and control groups using the test sample (sulfur hot spring water) and the control sample (Arisu). Therefore, when normality is satisfied, descriptive statistics such as mean and standard deviation are presented as parametric statistics and compared by verifying with Student's t-test. If the assumption of normality is not satisfied, descriptive statistics such as median and quartile deviation are presented as non-parametric statistics, and the Mann-Whitney U test is used. All statistical analyzes are conducted at a significance level of 5%.

2) Analysis of secondary efficacy evaluation variables

Among the secondary efficacy evaluation variables, the change in itching in visit1 and visit2 for the test group and control group applies the primary efficacy evaluation variable analysis method. In addition, the amount of change in transepidermal water loss, erythema measurement, skin roughness, and dead skin cells according to the measurement period from visit1 to visit2, and from visit1 to visit3, was determined by mixed design two-way ANOVA if the basic assumptions of parametric statistics were satisfied. When detailed information on post-hoc comparison is required depending on the purpose of the study, student's (independent) t-test is used for comparison between groups at each measurement period and the number of visits in each group (group). The amount of change in the secondary effectiveness evaluation score is analyzed using a paired (repeated) t-test. If the basic assumptions are not satisfied, the median and quartile deviation are presented as descriptive statistics and analyzed using non-parametric statistical methods, Mann-Whitney U test and Wilcoxon signed-rank test. Additionally, in the case of categorical variables, the Chi-Square Test or Fisher's exact test is applied for analysis.

3) Analysis method for safety variables

For each adverse reaction, the number and percentage of subjects who experienced one or more adverse reactions are described divided into test group and control group. The degree, measures, and results of adverse reactions are also classified by test group and control group, and the number of subjects, percentage, and frequency of occurrence are described. The incidence of adverse reactions and serious adverse reactions is summarized for each group and tested using the chi-square test or Fisher's exact test.

The definition of adverse reactions is as follows.

i) Adverse reactions

- Refers to undesirable and unintended signs, symptoms, or diseases occurring in subjects who used the sample, and does not necessarily have a causal relationship with the sample.

ii) Serious AE / Serious AE

This refers to any of the following adverse reactions occurring at a certain dose, or other medically important situations that require action to prevent the listed results from occurring.

- When it causes death or threatens life

- When hospitalization or extension of hospitalization period is necessary

- If it causes persistent or significant disability or functional decline (if it causes permanent or significant disability or functional decline)

- If it causes congenital deformities or abnormalities

iii) Predicted adverse events

- Itching, stinging, burning, swelling, etc.

iv) Evaluation of adverse events

If an adverse reaction occurs, it is evaluated according to the severity evaluation criteria in Table 7 below.

8. Test results

1) General characteristics of subjects between the two groups before the test

Differences between groups were evaluated in baseline characteristics before application of test samples in the PP group and the ITT group. As a result, no statistically significant differences were observed in age, height, weight, systolic blood pressure, diastolic blood pressure, heart rate, body temperature, VAS, etc. in both the test and control groups, and the same variance between the two groups was observed for all evaluation variables including VAS. By confirming, it can be said that the prior homogeneity of the two groups is recognized, and the random assignment is judged to be statistically appropriate (Table 8 below shows the ITT group and Table 9 shows the PP group)

2) Analysis of effectiveness evaluation variables (Modified ITT)

i) Results of primary efficacy endpoints

- Itching was significantly reduced in visit3 compared to visit1 in both the test and control groups, but there was no significant difference between groups (see Table 10 below)

Below, the results of the secondary efficacy evaluation variables are described.

ii) Change in pruritus from visit1 to visit2

- Itching was significantly reduced in visit2 compared to visit1 in both the test and control groups, but there was no significant difference between groups (see Table 10 below)

iii) Changes in skin moisture content from visit1 to visit2 and from visit1 to visit3 measured with a corneometer

- The interaction effect of skin moisture content depending on group and measurement period was statistically significant. In the test group, there was a significant increase in visit2 and visit3 compared to visit1, while there was no significant change in the control group (see Table 11 below).

iv) Changes in transepidermal water loss (Tewameter) from visit1 to visit2 and from visit1 to visit3

The interaction effect between transepidermal water loss and group and measurement period was statistically significant. In the test group, there was a significant decrease in visit2 and visit3 compared to visit1, while there was no significant change in the control group (see Table 11 below).

v) Changes in skin roughness (VisioScan VC20 PLUS) from visit1 to visit2 and from visit1 to visit3

Skin roughness decreased in visit2 compared to visit1 in both the test and control groups, but not significantly, and significantly decreased in visit3 compared to visit1. There was no significant difference between groups (see Table 11 below).

vi) Changes from visit1 to visit2 and from visit1 to visit3 in erythema (Mexameter MX18)

For erythema, the interaction effect between group and measurement period was statistically significant. In the test group, there was a significant decrease in visit2 and visit3 compared to visit1, while there was no significant change in the control group (see Table 11 below).

vii) Changes from visit1 to visit2 and from visit1 to visit3 in keratin (VisioScan VC20 PLUS)

For keratin, the interaction effect between group and measurement period was statistically significant. In the test group, there was a significant decrease in visit2 and visit3 compared to visit1, while there was no significant change in the control group (see Table 11 below).

3) Analysis of effectiveness evaluation variables (PP)

i) Results of primary efficacy endpoints

- Itching was significantly reduced in visit3 compared to visit1 in both the test and control groups, but there was no significant difference between groups (see Table 12 below)

Below, the results of the secondary efficacy evaluation variables are described.

ii) Change in pruritus from visit1 to visit2

- Itching was significantly reduced in visit2 compared to visit1 in both the test and control groups, but there was no significant difference between groups (see Table 12 below)

iii) Changes in skin moisture content from visit1 to visit2 and from visit1 to visit3 measured with a corneometer

- The interaction effect of skin moisture content depending on group and measurement period was statistically significant. In the test group, there was a significant increase in visit2 and visit3 compared to visit1, but there was no significant change in the control group (see Table 13 below).

iv) Changes in transepidermal water loss (Tewameter) from visit1 to visit2 and from visit1 to visit3

The interaction effect between transepidermal water loss and group and measurement period was statistically significant. In the test group, there was a significant decrease in visit2 and visit3 compared to visit1, while there was no significant change in the control group (see Table 13 below).

v) Changes in skin roughness (VisioScan VC20 PLUS) from visit1 to visit2 and from visit1 to visit3

Skin roughness decreased in visit2 compared to visit1 in both the test and control groups, but not significantly, and significantly decreased in visit3 compared to visit1. There was no significant difference between groups (see Table 13 below).

vi) Changes from visit1 to visit2 and from visit1 to visit3 in erythema (Mexameter MX18)

For erythema, the interaction effect between group and measurement period was statistically significant. In the test group, there was a significant decrease in visit2 and visit3 compared to visit1, while there was no significant change in the control group (see Table 13 below).

vii) Changes from visit1 to visit2 and from visit1 to visit3 in keratin (VisioScan VC20 PLUS)

For keratin, the interaction effect between group and measurement period was statistically significant. In the test group, there was a significant decrease in visit2 and visit3 compared to visit1, while there was no significant change in the control group (see Table 13 below).

4) Stability evaluation

Adverse reactions occurred in 1 person out of 30 people in the test group, showing an incidence of 3.4%. There was no statistically significant difference in adverse reactions between the test group and the control group.

9. Sintering

In this experimental example, the evaluation was conducted by analyzing and comparing itching (VAS), moisture loss, moisture content, skin roughness, erythema, and dead skin cells to determine whether there was a statistically significant difference. The same results were found in the Modified ITT group and the PP group.

As a result of analyzing the itching sensation set as the primary efficacy evaluation variable, it was found that the itching sensation was statistically significantly reduced after use compared to before use in both the test and control groups, and statistical significance could not be confirmed in the comparison between groups.

The results of the analysis of the items set as secondary effectiveness evaluation variables are as follows.

Skin moisture content significantly increased in the test group before and after using the sample, while there was no significant change in the control group.

Transepidermal moisture loss, erythema, and keratin significantly decreased in the test group after using the sample compared to before, while there was no significant change in the control group.

Skin roughness was found to be statistically significantly reduced after sample use in both the test and control groups. Statistical significance could not be confirmed in the comparison between the test group and the control group.

In summary, it was confirmed that the hot spring water composition according to the example had an effect of alleviating itching and accompanying symptoms (scalyness, roughness, erythema) and a moisturizing effect on itchy skin. Statistically significant improvement effects were confirmed in moisture loss, moisture content, skin roughness, erythema, and dead skin cells. Although there was no statistical significance in itching, it appeared to be significantly reduced compared to the control group.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the following claims are also possible. falls within the scope of rights.

Claims (5)

The powder manufactured by powdering the hot spring water composition containing hot spring water taken from 138-32 Seokjang-gil (137 Seokjang-ri), Gaegun-myeon, Yangpyeong-gun, Gyeonggi-do, Republic of Korea, is uniformly dispersed,
The sulfur content of the hot spring water is 3.99 mg/L,
The hot spring water composition contains 0.43 mg/L of K, 143 mg/L of Na, 2.49 mg/L of Ca, less than 0.02 mg/L of Mg, 33.0 mg/L of SiO ₂ , 0.12 mg/L of Li, 0.20 mg/L. /L Sr, less than 0.03 mg/L Fe, less than 0.01 mg/L Mn, less than 0.03 mg/L Cu, less than 0.03 Pb, less than 0.05 mg/L Zn, 19.9 mg/L F ^- , 119 Contains mg/L Cl ^- , and 9.97 mg/L SO ₄ ^2- ,
The powder includes two or more types of powders with different average diameters.
Gun tissue.
According to paragraph 1,
Dry tissues where the average diameter of the powder is 0.05 to 50 ㎛.
delete A separate wet tissue containing the dry tissue of any one of paragraphs 1 or 2.
According to paragraph 4,
The detachable wet tissue is a detachable wet tissue for improving or preventing atopic dermatitis, or for cosmetic purposes.