KR102633789B1 - Multi-purpose multi-well device with vacuum chamber - Google Patents

Abstract

The present invention prevents the fluid extraction passage from being blocked by the filter by suction force when extracting a target extract from a fluid sample containing a chemical fluid or biological organism by passing through a plurality of membrane layers (hereinafter referred to as "filters") and improves the viscosity of the fluid sample. Ultimately, by improving the flowability of the extract in response to the characteristics, a uniform amount of extract can be extracted from the fluid sample in each well, and the target extract can be extracted from the fluid sample using either gravity or artificial suction force. The flow rate of the fluid sample can be naturally measured by discharging the excessive water input into the well, and the work of receiving the target extract from the fluid sample and transferring it to the analysis device can be performed at the same time. It relates to a multi-purpose multi-well device including a vacuum chamber.

Description

{MULTI-PURPOSE MULTI-WELL DEVICE WITH VACUUM CHAMBER}

The present invention relates to a multi-purpose multi-well device comprising a vacuum chamber for extracting titrated and/or filtered precipitates or extracts from chemical fluids or fluid samples containing biological organisms, further comprising: Specifically, when extracting the target extract from a fluid sample containing a chemical fluid or biological organism by passing a plurality of membrane layers (hereinafter referred to as "filters"), the fluid extraction passage is prevented from being blocked by the filter due to suction force, and the fluid sample By improving the flowability of the extract in response to the viscosity characteristics of the extract, ultimately, the extract can be uniformly extracted from the fluid sample in each well, and the desired extract can be extracted from the aforementioned fluid sample by either gravity or artificial suction force. The flow rate of the fluid sample can be naturally measured by discharging the excessive water input into the well, and the vacuum is capable of receiving the target extract from the fluid sample and transferring it to the analysis device at the same time. A multi-purpose multi-well device comprising a chamber.

A multi-well device consists of a deep space surrounded by walls on all sides and has a standard number of wells. When vacuum or air pressure is applied to one side of the well device, the fluid sample in each well is drawn by suction force. This includes a filter that is intended to pass through.

The filter described above is employed to remove one or more specific components from solutions passing through the filter based on physical, biological and/or chemical interactions within or on the surface of the filter.

These interactions may take place, for example, between the aforementioned filter and the component to be retained, or between one or more substances retained on the interior or surface of the filter and the component to be retained.

This allows the multi-well devices described above to be used to remove one or more undesirable or, conversely, desirable substances from a fluid sample.

The above-mentioned multi-well device is described in detail in Korean Patent Publication No. 10-2008-0083721 (2008.09.18) and Korean Patent Publication No. 10-2009-0045426 (2009.05.07), and the above-mentioned filter is described in Korean Patent Publication No. 10-2022- Please refer to this as detailed in 0142960 (2022.10.24).

The above-mentioned published patents 10-2008-0083721 and 10-2009-0045426 disclose that in the case of fluid samples with high fluid viscosity (or concentration), the flow from some wells to the fluid flow port is delayed due to the problem of reduced flowability of the extract. Problems may arise such as backflow of fluid samples upstream of the filter, which not only presents a distinct problem of variation in the amount of extract extracted from each well, but also causes fluid flow through the filter due to suction force in a particular well. Port blockage may occur, and adverse effects may occur when handling filters in the well and/or processing fluid samples after extracting the target extract.

In addition, the known well device described above has a problem in that the vial that receives the target extract from the fluid sample does not meet the specifications required by the analysis device, so the extracted extract must be transferred to the vial required by the analysis device.

The present invention was created to solve the problems described above, and has achieved remarkable results, so we intend to propose this through the present invention.

The object of the present invention is to discharge the excessive amount of water introduced into the well without blocking the fluid passage by the filter when extracting the target extract from a fluid sample containing a chemical fluid or biological organism by passing it through a filter. The aim is to provide a multi-purpose multi-well device including a vacuum chamber capable of naturally metering flow.

Another object of the present invention is to provide a multi-purpose multi-well device that can receive a target extract from a fluid sample and immediately analyze it through an analysis device.

Another object of the present invention is to provide a multi-purpose multi-well device that can ultimately extract a uniform amount of extract from fluid samples in each well by improving the drainage efficiency of the extract passing through the filter layer.

Another object of the present invention is to provide a multi-purpose multi-well device including a vacuum chamber capable of extracting a desired extract from a fluid sample by either gravity or suction force.

In the present invention, a multi-well device includes a vacuum chamber 400 including a fluid receiving plate 100, a filter plate 200, and a vial plate 300, and a cover plate for airtightening each well of the fluid receiving plate. More may be included.

The above-described fluid receiving plate 100,

A cover plate 120 accommodating part 101a (FIG. 1) whose height is parallel to the upper surface 101, a side wall 102 forming a square shape by opposing surfaces, and a standard structure within the aforesaid accommodating part 101a comprising wells 110 (FIGS. 1-4, 6) for receiving fluid samples in adequate quantities;

Each of the above-described wells 110 has an upper bore 111 (FIG. 3) of which the inner wall 111a with an open top has a square cross-section, and an inner wall 111a of the above-mentioned upper bore 111 downward in the inward direction. An inclined collar 114 (collar, FIGS. 3 and 6) and a lower portion extending downward from the lower part of the aforementioned collar 114 in an annular shape and the outer wall of which is airtightly coupled to the well inner wall 113a of the filter plate 200. Including bore 113 (FIGS. 3 and 6),

The above-described lower bore (113, FIGS. 3, 6) is disposed in the horizontal direction from the end of the inner wall (113a, FIG. 7) and pressurizes only the edge portion of the filter (500, FIG. 27) (FIG. 6). , comprising a bottom layer (113d, FIG. 7) including (FIG. 7),

The above-described filter plate 200 (FIGS. 8 to 12),

The inner wall 212a (FIGS. 8, 9), which airtightly accommodates the outer wall of the lower bore 113 (FIGS. 3, 6), and the opposing surface of the filter 500 facing the pressurizing portion 113b described above. It is provided with a support portion (213a, FIG. 13), an inclined surface (213b) inclined at a predetermined angle from the above-described support portion (213a) toward the suction passage (214, FIG. 12), and the above-mentioned suction passage (214), and the inclined surface ( a filter plate well 210 (FIGS. 8, 9, 12) comprising a bottom layer 213d located at the center of 213b) and having a suction passage 214 for extracting the extract of interest from the fluid sample;

An overwater storage portion 203 partitioned to a predetermined depth by a partition wall 201a (FIG. 8) disposed between each of the above-mentioned wells 210 and between each well 210 and the side plate 201 of the filter plate 200, 8, 9) and;

An antifouling skirt 205 (FIG. 8) extends longer than the length of the suction passage 214 described above on the bottom of the well 210 of the filter plate 200 and surrounds the circumference of the suction passage 214 of each well 210. to Figure 12), but

The above-described inclined surface 213b (FIGS. 12 and 13) is,

Bosses 216 (FIGS. 12, 13) that protrude from the inclined surface 213b at a predetermined height and support the filter 500 so that the bottom of the filter 500 is parallel to the support portion 213a, and the suction passage A plurality of fluid flow passages (213c, FIG. 13) connected to (214) and a fluid distribution passage (213c, FIG. 13) disposed radially between the adjacent fluid flow passages (213c, FIG. 213d, Figure 13),

The above-described vacuum chamber 400 (FIGS. 1, 25) includes a replaceable vial tray 300 (FIG. 21), is hermetically coupled to the lower side plate of the fluid receiving plate, and is sequentially connected to the sealing plate 410 (FIG. 22, FIGS. 25), a spacer 420 (FIGS. 23, 25), a chamber body 430 (FIGS. 24, 25),

The sealing plate 410 (FIGS. 22, 25) has an opening 412 (FIG. 22) for accommodating the outermost antifouling skirt 205 described above, and is provided around the opening 412 on the bottom surface. Includes a sealing member (413, Figure 22) and an alignment hole (414, Figure 22),

The spacer 420 (FIGS. 23 and 25),

It includes an alignment pin (424, FIG. 23) matched to the alignment hole (414) on the upper surface, and a space portion (422, FIG. 23) communicating with the opening portion (412, FIG. 22). A step (425, FIG. 23) that presses the upper edge surface of the vial tray 300 on the edge of FIG. 23) and a sealing member that extends downward on the bottom surface and is disposed along the lower periphery of the space portion (422, FIG. 23) comprising a key 426a comprising (426, FIG. 23);

The chamber body 430 (FIG. 24) is,

It is equipped with the vial tray receiving portion 432 (FIG. 24) connected to a vacuum pump (not shown in the drawing), and has a key groove (426a) having a shape corresponding to the key 426a described above around the upper periphery of the receiving portion 432. 431, Figure 24),

The above-described vial tray 300 (FIG. 21),

It includes a top plate 302 having perforations 301 (FIG. 21) into which each vial (310, FIG. 21) can be independently inserted, and a side plate 303 constituting the height of the above-described top plate 302,

The above-described cover plate 120 (Figure 1),

Stoppers 121 (FIG. 26) having a shape corresponding to the inner wall of the above-described upper bore 111 (FIG. 6), and a plate-shaped member (not shown) that airtightly overlaps the upper surface of the fluid receiving plate 100. It is characterized by including.

As an example, the collar 114 (FIG. 6) described above may further include an overflow hole 114a (FIGS. 6 and 7) through which an excessive amount of fluid sample can be discharged.

According to the present invention, each well of the fluid receiving plate is independently airtight by a cover plate, and when both wells of the fluid receiving plate and the filter plate are assembled, only the edges of the filter are airtightly fixed in the well by the pressurizing portion and the support portion. Not only that, but the boss supports the filter so that the bottom of the filter layer is spaced apart from the inclined surface, thereby securing space for forming a fluid flow system at the bottom of the filter.

Therefore, the fluid sample can pass through the filter layer evenly without flowing back from a specific well due to gravity or suction force, and the problem of the fluid flow passage and suction passage being blocked by the filter does not occur.

In addition, according to the present invention, when filtering a target extract from a fluid sample with high viscosity, the slowly flowing extract flowing along the fluid flow passage is dispersed and flows along the fluid distribution passage, thereby eliminating the problem of fluid flowing back into the filter or upstream thereof. does not occur

In addition, in the present invention, the suction passage of the filter plate is surrounded by an anti-fouling skirt, and is configured to be smaller than the height of the anti-fouling skirt, so there is no problem of contamination of the end portion.

In addition, the present invention allows vial trays with different perforation sizes to be selectively placed in the chamber by replacing the spacer to suit the diameter and height of the vial, so that not only is one well device compatible with various vial trays, but also extracts can be extracted. You can receive it and transfer it to the analysis device at once.

Therefore, a multi-purpose multi-well device including a vacuum chamber employing either a gravity or negative pressure method or a combination of both methods is provided.

1 is a perspective view of a multi-well device illustrating a preferred embodiment according to the present invention.
FIG. 2 is a perspective view showing only the fluid receiving plate of the multi-well device shown in FIG. 1.
FIG. 3 is a partially cut perspective view of the fluid receiving plate cut along line AA shown in FIG. 2.
Figures 4 and 5 are top and bottom views of the fluid receiving plate shown in Figure 2.
Figure 6 is a cross-sectional view of the fluid receiving plate cut along line BB in Figure 4.
Figure 7 is an enlarged view of the well corresponding to area H in Figure 4.
Figure 8 is a perspective view showing only the filter plate of the multi-well device shown in Figure 1.
Figures 9 to 11 are a top view, a front view, and a bottom view of the filter plate shown in Figure 8.
Figure 12 is a cross-sectional view taken along line CC of Figure 9.
Figure 13 is an enlarged view of part I in Figure 9.
Figure 14 is a perspective view showing the assembled state of the fluid receiving plate and filter plate shown in Figures 2 and 8.
Figures 15 to 17 are top, front and bottom views of the assembled fluid receiving plate and filter plate shown in Figure 14.
Figure 18 is a perspective view showing an excerpt of the well of the filter plate shown in Figure 9.
FIG. 19 is a cross-sectional view showing the main part of the well shown in FIG. 18.
FIG. 20 is a cross-sectional view taken along line DD shown in FIG. 15.
FIG. 21 is a perspective view illustrating a vial tray extracted from the multi-well device shown in FIG. 1.
FIGS. 22A to 22C are a spacer of a vacuum chamber in the multi-well device shown in FIG. 1, a cross-sectional view along line EE, and a bottom view of the spacer.
FIGS. 23A to 23C are a top view, a cross-sectional view along the line FF, and a bottom view of the main body of the vacuum chamber of the multi-well device shown in FIG. 1.
FIGS. 24A to 24C are a plan view, a cross-sectional view along line GG, and a bottom view of the main body of the vacuum chamber of the multi-well device shown in FIG. 1.
Figure 25 is a cross-sectional view showing an example of combination of the sealing plate, spacer, and full body shown in Figures 22b, 23b, and 24b.
Figure 26 is a main sectional view showing an example of use of the fluid receiving plate, filter plate, and vial according to the present invention.
Figure 27 is a schematic cross-sectional view showing the process for extracting extract from a fluid sample using the multi-well device of the present invention.

All references, including publications, patent applications, and patents, cited herein are the same as if each reference were individually and expressly indicated to be incorporated by reference and were set forth in its entirety herein. To this extent, they are incorporated herein by reference.

In the context of describing the invention (and especially in the context of the claims below), the use of the singular referents and "at least one" and similar referents refers to both the singular and the plural, unless otherwise indicated herein or clearly contradictory from the context. It is interpreted as including. The use of the term “at least one” following a list of one or more items (e.g., “at least one of A and B”) means that the listed items, unless otherwise indicated herein or otherwise clearly contradicted by context, refer to the listed items. It should be interpreted to mean one item (A or B) selected from, or a combination of two or more of the listed items (A and B).

Recitation of ranges of values herein is intended to serve as a shorthand way of referring individually to each individual value falling within the range, unless otherwise indicated herein, and each individual value is referred to herein as if it were a separate value. are incorporated herein as if individually indicated. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context.

The use of any or all examples or exemplary language (e.g., “such as”) provided herein is merely to better illustrate the invention and does not limit the scope of the invention unless otherwise claimed. . No language in the specification should be construed as indicating that any non-claimed element is essential to the practice of the invention.

The present invention can make various changes and have various embodiments, and specific embodiments will be illustrated in the drawings and described in detail here.

However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all changes, equivalents, and substitutes included in the spirit and technical scope of the present invention.

Hereinafter, with reference to the attached drawings, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention.

However, since the description of the present invention is only an example for functional explanation, the scope of the present invention should not be construed as limited by the examples described in the text.

For example, since the embodiments are capable of various changes and may have various forms, the scope of rights of the present invention should be understood to include equivalents that can realize the technical idea.

In addition, the purpose or effect presented in the present invention does not mean that a specific embodiment must include all or only such effects, so the scope of the present invention should not be understood as limited thereby.

In this specification, the present embodiments are provided to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the scope of the invention. And the present invention is only defined by the scope of the claims.

Accordingly, in some embodiments, well-known components, well-known operations, and well-known techniques are not specifically described to avoid ambiguous interpretation of the present invention.

Meanwhile, the meaning of the terms described in the present invention is not limited to the dictionary meaning and should be understood as follows.

All terms used in the present invention, unless otherwise defined, have the same meaning as commonly understood by a person of ordinary skill in the field to which the present invention pertains.

In general, terms defined in used dictionaries should be interpreted as consistent with the meaning they have in the context of the related technology, and cannot be interpreted as having an ideal or excessively formal meaning unless clearly defined in the present invention.

Hereinafter, embodiments disclosed in the present specification will be described in detail with reference to the attached drawings. However, identical or similar components will be assigned the same reference numbers regardless of reference numerals, and duplicate descriptions thereof will be omitted.

The components used in the following description are given or used interchangeably considering only the ease of preparing the specification, and do not have distinct meanings or roles in themselves.

Additionally, in describing the embodiments disclosed in this specification, if it is determined that detailed descriptions of related known technologies may obscure the gist of the embodiments disclosed in this specification, the detailed descriptions will be omitted.

In addition, the attached drawings are only for easy understanding of the embodiments disclosed in this specification, and the technical idea disclosed in this specification is not limited by the attached drawings, and all changes included in the spirit and technical scope of the present invention are not limited. , should be understood to include equivalents or substitutes.

The present invention will be described in detail with reference to the attached drawings of a multi-purpose multi-well device including a vacuum chamber. In the drawings attached to the description, like elements have the same reference numerals.

The multi-purpose multi-well device includes a vacuum chamber (400) in which a fluid receiving plate (100, Figures 2 to 7), a filtration plate (200, Figures 8, 9), and a vial tray (300, Figure 21) are placed. , FIGS. 1 and 25), and may further include a cover plate 120 (FIGS. 1 and 25) for airtightening each well 110 of the above-described fluid receiving plate 100.

The above-mentioned fluid receiving plate 100 is configured in a square shape by the cover plate 120 receiving portion 101a, which accommodates the cover plate 120 and constitutes a surface parallel to the cover plate 120, and the opposing surface. It includes a side wall 102 (FIGS. 2 to 4) and wells 110 (FIG. 4) for receiving fluid samples in a standard quantity within the above-described receiving portion 101a.

Each of the above-described wells 110 has an upper bore 111 with an open upper inner wall 111a (FIG. 6) having a square cross-section, and an inner wall 111a of the upper bore 111 that extends downward in the inward direction. A highly inclined collar 114 (FIG. 6) and an outer wall 113a (FIG. 3) extending downward from the collar 114 are in contact with the inner wall 113a of the well 210 (FIG. 8) of the filter plate 200 (FIG. 8). It includes a lower bore 113 (FIG. 3) that is tightly coupled.

The above-mentioned lower bore 113 is provided with a bottom layer 113d including a pressing portion 113b (FIGS. 3 and 6) that is arranged horizontally from the end of the inner wall and presses only the edge portion of the filter 500, and the adjacent A space 113e is provided between the outer walls (not indicated) of one lower bore 113.

This space 113e is located at the periphery of the well 210 of the filter plate 200 (not indicated by reference numeral).

The filter plate 200 (FIGS. 8 to 13) described above includes an inner wall 212a that airtightly accommodates the outer wall (not indicated) of the lower bore 113 described above, and an inner wall 212a provided at the end of the inner wall 212a described above. A support portion (213a, Fig. 13) supporting the opposing surface of the filter 500 facing the filter pressing portion (113b), and a predetermined angle from the above-described support portion (213a) toward the suction passage (214, Figs. 12 and 13). A filter plate well (210) comprising an inclined surface (213b) and a bottom layer (213d) located at the center of the above-described slope (213b) and having a suction passage (214) for extracting the target extract from the fluid sample;

An overwater storage portion 203 partitioned to a predetermined depth by a partition 212b (FIG. 8) disposed between each of the above-described wells 210 and between each well 210 and the side plate 201 of the filter plate; and

An antifouling skirt 205 (FIGS. 8, 10) extends longer than the length of the suction passage 214 described above on the bottom of the above-described filter plate well 210 and surrounds the circumference of the suction passage 214 of each well 210. , Figure 12).

The upper peripheral portion of each well 210 of the above-described filter plate is preferably configured to be lower than the height of the upper portion of the side wall 202. This makes it possible to hermetically couple the wells of the fluid receiving plate 100 to the wells of the filter plate containing the filter, corresponding to the thickness of the selectively employed filter layer.

The above-described suction passage 214 is provided in each well 210, and is provided to extend downward from each well 210 to a predetermined length.

In this way, regardless of whether it is a bottle-shaped vial, a capsule-shaped vial, or a dish-shaped vial, the suction passage 214 can be placed within the vial (310, Figure 21, Figure 26), and the extract is contained only into the vial without leaking out. You can lose.

The antifouling skirt 205 described above is longer than the length of the suction passage 214 of each well 210, and is configured to independently surround the circumference of each suction passage 214.

In this way, since the end of the suction passage 214 of the dismantled filter plate 200 does not contact the ground, the end of the suction passage 214 can be prevented from being contaminated by various contaminants.

The above-described inclined surface 213b (FIGS. 13, 18) protrudes from the inclined surface 213b at a predetermined height so that the bottom surface of the above-described filter 500 (FIG. 27) is parallel to the surface of the above-described support portion 213a, thereby forming a filter. Between the bosses 216 supporting the bottom of the, a plurality of fluid flow passages 213c (FIGS. 13, 18) connected to the suction passage 214 described above, and the adjacent fluid flow passages 213c described above. It includes a fluid distribution passage (213d) disposed in the radial direction and connecting both passages (213c).

The depth of the above-described fluid flow passage 213c may be the same as the depth of the fluid distribution passage 213d, and more preferably, the depth of the fluid flow passage 213c is provided to be relatively deeper.

As shown in FIGS. 3, 4, 6, and 7, the above-described pressing portion 113b is provided with a square-shaped opening portion 113c in the center to uniformly press and compress only the edge portion of the filter 500. You can.

The above-described bosses 216 may be provided in plurality between the fluid flow passages 213c.

The above-described fluid flow passage 213c is a passage that guides the extract that has passed through the layer of the filter 500 to be discharged into the suction passage 214, and the fluid distribution passage 213d is a fluid sample with low flowability, that is, viscosity. It is a passage that distributes (or disperses) the extract extracted from.

Therefore, the extract extracted from the weakly viscous fluid sample can flow along the fluid flow passage 213c, and the extract extracted from the viscous fluid sample can be dispersed and flow through the fluid distribution passage 213d due to the delay in flow.

In FIG. 18, an unillustrated reference numeral 212c denotes a junction where two adjacent wells 210 are joined.

The vacuum chamber 400 (FIGS. 1, 25) described above includes an exchangeable vial tray 300 (FIG. 21), and is provided with a rubber material located on the lower peripheral portion 102a of the side wall of the fluid receiving plate 200 (see The sealing plate 410, the spacer 420, and the chamber body 430 are coupled to be airtight by (not shown).

The sealing plate 410 described above has an opening 412 (FIG. 22) for accommodating the outermost antifouling skirt 205 described above, and a sealing member 413 provided around the opening 412 on the bottom surface. and an alignment hole 414 (FIG. 22).

The above-described spacer 420 is provided with an alignment pin (424, Figure 23b) on the upper surface that matches the alignment hole 414, and has a space portion (422, Figure 23b) passing through the above-mentioned opening 412. It includes a step 425 (FIG. 23b) on the edge of the space 422 in contact with the edge of the upper surface of the vial tray 300, and a seal extending downward on the bottom and disposed along the lower periphery of the space 422. It includes a key 426a including a member 426.

The above-described spacer 420 can be changed to the vial tray 300 that can fit the shape of the vial 310 (FIG. 21), that is, the length that allows the end of the suction passage 214 to enter the inside of the vial 310. there is.

The chamber body 430 is equipped with the vial tray receiving portion 432 (FIG. 24), which is connected to a vacuum pump (not shown in the drawing) to create negative pressure, and the above-mentioned vial tray storage portion 432 (FIG. 24) is formed around the upper periphery of the receiving portion 432. It includes a key groove 431 having a corresponding shape that can be engaged with the key 426a.

The upper surface of the above-mentioned sealing plate 410 is coupled to be sealed by installing a rubber plate (not shown in the drawing) on the lower part of the side plate of the fluid receiving plate 200, and the bottom of the above-mentioned sealing plate 410 is connected to a spacer ( It can be assembled and disassembled by fastening the upper surface of 420) and bolts (not shown).

In addition, the above-described spacer 420 is airtightly joined to the chamber body 430 by a sealing member when the key and keyway are aligned.

In FIG. 24, an unillustrated reference numeral 401 denotes a piping connection port connected to a vacuum pump (not shown in the drawing).

The above-described vial tray 300 (FIG. 21) has an upper plate 302 having independent perforations 301 into which each independent vial 310 can be individually inserted, and a height of the above-described upper plate 302. Includes a side plate 303.

The above-mentioned cover plate 120 includes stoppers 121 having a shape corresponding to the inner wall of the above-described upper bore, as shown in FIG. 26, and is parallel to the upper surface of the fluid receiving plate 200. It can be configured.

As an example, the collar 114 (FIG. 7) described above may further include an overflow hole 114a (FIGS. 6 and 7) through which an excessive amount of fluid sample can be discharged.

In this way, the excess fluid sample injected into the well of the fluid receiving plate 100 is discharged through the overflow hole 114a, so that the same effect as if the amount of fluid sample injected into each well is measured and injected can be obtained. .

And, the overwater discharged through the above-described overflow hole 114a is contained in each storage portion 103 of the filter plate 200.

The above-mentioned storage unit 203 is a space with a predetermined depth closed at the bottom by a partition of the partition wall 201a between the neighboring wells 210 on the filter plate 200 and between each well 210 and the side wall 202. am.

As described above, the present invention can receive the target extract from the fluid sample through a vial suitable for the analysis device by using a vial tray installed in the vacuum chamber, and can directly input it into the analysis device. So, you can perform the above series of tasks at once.

The well device according to the present invention is capable of filtering extracts by gravity by assembling a fluid receiving plate and a filter plate, and is multi-purpose in that it can extract a target extract from a fluid sample using suction force by using a vacuum chamber in combination.

Although filters are not mentioned in detail in the present invention, filters for extracting titrated and/or filtered precipitates or extracts from chemical fluids or fluid samples containing biological organisms are known (Korean Patent Publication No. 10-2022-0142960 (2022.10.24 ), so please use a filter that suits your purpose.

Preferred embodiments of the invention have been described herein, including the best mode known to the inventors for carrying out the invention.

Applications or modifications according to these preferred embodiments can be easily made by anyone skilled in the art through the detailed description of the present specification, but these application examples or modifications are not intended by the present inventor. We make it clear in advance that this is included in the true technical idea and scope of the patent claims.

The inventors expect skilled artisans to employ such variations as appropriate, and the inventors do not intend for the invention to be practiced otherwise than as explicitly described herein.

Accordingly, this invention includes all modifications and equivalents of the subject matter recited in the claims appended hereto as permitted by applicable law.

Moreover, any combination of the above-described elements in all possible variations thereof is encompassed by the invention unless otherwise indicated herein or otherwise clearly contradicted by context.

delete

delete

delete

Claims (9)

It includes a vacuum chamber that retractably accommodates a cover plate, a fluid receiving plate, a filter plate, and a vial tray that are sequentially overlapped to be airtight;
The vacuum chamber is,
In order, it includes a sealing plate, a spacer, and a chamber body that are airtightly coupled to the lower side plate of the fluid receiving plate,
The sealing plate is,
It has an opening for receiving the outermost antifouling skirt, and includes a sealing member and an alignment hole provided around the opening on the bottom,
The spacer is,
It includes an alignment pin provided on the upper surface and matched to the alignment hole, and a space communicating with the opening,
Sealing the edge of the vial tray to the edge of the space, and comprising a sealing member extending downward from the bottom and disposed along the lower periphery of the space,
The chamber body is,
A multi-purpose multi-well device comprising the vial tray storage unit connected to a vacuum pump.
According to paragraph 1,
Each well of the fluid receiving plate,
A multi-purpose multi-well device, characterized in that it is independently airtight by the cover plate.
According to paragraph 2,
The cover plate is,
Stoppers having a shape corresponding to the inner wall of the upper bore,
A multipurpose multi-well device comprising a plate-shaped member that airtightly overlaps the upper surface of the fluid receiving plate.
According to paragraph 1,
The fluid receiving plate is,
Constructing rectangular side walls by opposing surfaces, comprising a plurality of wells containing fluid samples in standard quantities,
Each of the wells has an upper bore with an open top and a square cross-section,
a collar inclined downward in an inward direction from the inner wall of the upper bore;
It extends downward from the lower part of the collar in an annular shape, and includes a lower bore whose outer wall is airtightly coupled to the inner wall of the well of the filter plate,
The lower bore is,
A multi-purpose multi-well device comprising a bottom layer including a pressurizing portion disposed horizontally from the end of the inner wall to pressurize the edge portion of the filter.
According to paragraph 4,
The fluid sample is,
A multi-purpose multi-well device characterized by either a chemical fluid or a biological organism.
According to paragraph 4,
The color is,
A multi-purpose multi-well device further comprising an overflow hole.
According to paragraph 1,
The filter plate is,
An inner wall that airtightly accommodates the outer wall of the lower bore, a support part supporting the opposing surface of the filter facing the pressurizing part, an inclined surface inclined at a predetermined angle from the support part toward the suction passage, and the suction passage, and a center of the inclined surface. a filter plate well including a bottom layer with a suction passage for extracting the extract of interest from the fluid sample;
an overwater storage portion partitioned to a predetermined depth by partition walls disposed between each well and between each well and the side plate of the filter plate;
An anti-fouling skirt extending on the bottom of the well of the filter plate longer than the length of the suction passage and surrounding the circumference of the suction passage of each well,
The slope is,
Bosses that protrude from the inclined surface at a predetermined height and support the filter so that the bottom of the filter is parallel to the support portion are disposed in the radial direction between a plurality of fluid flow passages connected to the suction passage and adjacent fluid flow passages. A multi-purpose multi-well device comprising a fluid distribution passage connecting both passages.
delete According to paragraph 1,
The vial tray is,
A multi-purpose multi-well device comprising a top plate including perforations into which each vial can be independently inserted, and a side plate that configures the height of the top plate.

Images