KR102631920B1 - A food composition comprising Neomysis awatschensis oil for anti-obesity - Google Patents

Abstract

As a new use of the insect, a method of using it as a food composition for anti-obesity is proposed. The present invention provides a food composition for anti-obesity containing chamomile oil.

Description

Food composition for anti-obesity comprising Neomysis awatschensis oil for anti-obesity}

The present invention relates to a food composition for anti-obesity, and more specifically, to a food composition for anti-obesity containing dandelion oil.

Along with economic development, our country's lifestyle has developed into that of advanced Western countries. As dietary habits change and physical activity decreases, body fat in the body increases and the number of obese people increases due to metabolic imbalance, resulting in diabetes, hyperlipidemia, and blood clots. The incidence of the so-called metabolic syndrome, which is a combination of cardiovascular diseases such as disability, is continuously increasing (Lee SJ, Park JY, Nam CM, Jee SH. 2008. The prevalence estimation of metabolic syndrome and it's related factors based on data from general health medical examination: a multi-center study. J Korean Soc Health Information Health Statistics 33: 119-133; Frohlich J, Lear SA. 2002. Old and new risk factors for atherosclerosis and development of treatment recommendations. Clin Exp Pharmacol Physiol 29 : 838-842).

Obesity is not a disease caused by a single cause, but a syndrome that is caused by a combination of genetic, environmental, and social factors. After excessive intake of energy, it is not consumed in the body's metabolic activities and is accumulated as neutral fat in adipose tissue. (Chua, S.C.Jr. Monogenic models of obesity. Behav Genet. 27: 277-284, 1997). In addition, increased blood fatty acid concentration and abdominal obesity seen in obesity are common symptoms of hyperlipidemia and type 2 diabetes, and increased free fatty acid causes ventricular hypertrophy (DeFronzo, R.A, Ferrannini, E. Insulin resistance. A multifaceted syndrome responsible for NIDDM, obesity, hypertension, dyslipidemia, and atherosclerotic cardiovascular disease. Diabetes Care. 14(3): 173-194, 1991), abdominal fat accumulation is closely related to causing insulin resistance. It has been reported (Tamori, Y., Kasuga, M. Obesity and insulin resistance. Nippon Rinsho. 67(2):236-244, 2009), which causes lifestyle diseases such as hypertension, arteriosclerosis, diabetes, and hyperlipidemia. This is becoming a factor that worsens health.

Neomysis awatschensis (Neomysis awatschensis) is a crustacean belonging to the Awatsidae family. It lives mostly in the sea and looks like a small shrimp. The body length is about 1 to 2 cm, and it differs from shrimp in that it has eight pairs of pectoral legs and has gills exposed at the base of the pectoral legs. It is caught in large quantities in the West Sea of Korea, and when pickled in salt, it is eaten as gonjaengijeot, and in places where it is caught in large quantities, it is also used as feed and fertilizer. There are about 20 species of gophers that live along the coast of Korea, with a body length of about 1 cm, and are also called 'jaha (紫蝦)'.

However, there have been no reported cases of using bugs in food compositions for anti-obesity yet.

In the present invention, we would like to propose a new use of gnats as a food composition for anti-obesity.

As an aspect for solving the above problems, the present invention provides a food composition for anti-obesity containing insect oil.

In addition, the insect oil provides a food composition for anti-obesity, characterized in that the phospholipid content is 60 to 70% by weight.

As another aspect for solving the above problem, the present invention includes the steps of (a) adding 10 to 20 times 90 to 100% (v/v) of alcohol to dried ragweed and extracting it; (b) first filtering the extract of step (a) and then first concentrating it under reduced pressure at 40 to 60°C; and (c) secondary filtration of the concentrate from step (b) and secondary concentration under reduced pressure at 50 to 100°C to produce oil with a viscosity of 700 to 1,000 cPs. to provide.

According to the present invention, it is possible to provide an anti-obesity food composition containing chamomile oil as a new use of a food composition using chamomile, and a method for producing chamomile oil to maximize the anti-obesity effect of chamomile.

Figure 1 is a photograph showing the raw material (a), hot air dried product (b), extract (c), primary concentrate (d), secondary concentrate (e), and insect oil (f) in the test example of the present invention. am.
Figure 2 is a photograph showing (a) insect oil and (b) garcinia as test substances used in animal testing.
Figure 3 is a graph showing the results of analyzing the effect of bug oil on the weekly body weight of an obese animal model caused by a high-fat diet.
Figure 4 is a graph showing the results of analyzing the effects of python oil on the weekly diet and water intake of an obese animal model caused by a high-fat diet.
Figure 5 is a graph showing the results of analyzing the effect of ragweed oil on the tissue weight of an obese animal model.
Figure 6 is a graph showing the results of analyzing the effect of ragweed oil on the concentrations of T-Chol, TG, LDL, and HDL in an obese animal model.
Figure 7 is a graph showing the results of analysis of the effect of bug oil on serum leptin concentration in an obese animal model.

Hereinafter, the present invention will be described in detail through preferred embodiments. Prior to this, the terms or words used in this specification and claims should not be construed as limited to their usual or dictionary meanings, and the inventor should appropriately define the concept of terms in order to explain his or her invention in the best way. Based on the principle of definability, it must be interpreted with meaning and concept consistent with the technical idea of the present invention. Therefore, the configuration of the embodiments described in this specification is only one of the most preferred embodiments of the present invention and does not represent the entire technical idea of the present invention, so various equivalents and modifications can be substituted for them at the time of filing the present application. You must understand that there may be. Additionally, throughout the specification, when a part is said to “include” a certain element, this means that, unless specifically stated to the contrary, it does not exclude other elements but may further include other elements.

In the present invention, significant results of weight loss, reduction in liver adipose tissue and epididymal fat, and reduction in blood cholesterol, LDL, and HDL contents were confirmed in animal tests of snail oil. Accordingly, the present invention discloses a food composition for anti-obesity containing chamomile oil.

In the present invention, in order to increase the anti-obesity efficacy related to the above indicators, python oil is prepared through (a) extraction step, (b) primary filtration and concentration step, and (c) secondary filtration and concentration step.

It was confirmed that the use of alcohol as an extraction solvent in step (a) was most effective in increasing anti-obesity efficacy, and it is preferable to extract by adding 10 to 20 times 90 to 100% (v/v) of alcohol to dried insects. do. In addition, in step (b), the extract from step (a) is first filtered and then first concentrated under reduced pressure, preferably at 40 to 60°C, and in step (c), the concentrated extract is second filtered and then second concentrated under reduced pressure. However, the anti-obesity effect can be maximized by producing the oil by concentrating it at 50 to 100°C until the viscosity reaches the level of 700 to 1,000 cPs.

The composition according to the present invention is provided in the form of a food composition and may include, for example, the form of functional food, nutritional supplement, health food, food additives, etc. there is. Food compositions of this type can be prepared in various forms according to conventional methods known in the art.

For example, as a health food, the composition itself can be prepared and consumed in the form of tea, juice, or drink, or can be consumed by granulating, encapsulating, or powdering. In addition, functional foods include beverages (including alcoholic beverages), fruits and their processed foods (e.g. canned fruit, bottled foods, jam, marmalade, etc.), fish, meat and their processed foods (e.g. ham, sausages, corned beef, etc.), and breads. and noodles (e.g. udon, buckwheat noodles, ramen, spaghetti, macaroni, etc.), fruit juice, various drinks, cookies, taffy, dairy products (e.g. butter, cheese, etc.), edible vegetable oil, margarine, vegetable protein, retort food, frozen. It can be manufactured by adding extracts to food, various seasonings (e.g. soybean paste, soy sauce, sauce, etc.). Additionally, in order to use the composition of the present invention in the form of a food additive, it can be prepared and used in the form of a powder or concentrate.

The preferred content of the extract or oil in the food composition according to the present invention may be 0.001 to 50%, preferably 0.1 to 10%, based on the total weight of the food composition.

Hereinafter, the present invention will be described in more detail through specific examples.

Mandarin oil production

30 kg of frozen insect organisms (raw material) (individual size about 1 cm) consisting of the nutrients shown in Table 1 below were thawed at room temperature and dried with hot air for 24 hours (50°C for 8 hours, 70°C for 8 hours, and 80°C for 8 hours). We prepared hot-air dried dried insects (yield 13%) consisting of the nutrients shown in Table 2. Afterwards, 15 times 95% (v/v) alcohol was added to the dried bean sprouts and extracted for 1 hour. Afterwards, the insect extract was first filtered using a filtration filter, first concentrated under reduced pressure at 50°C, secondly filtered again with a filter, and then secondarily concentrated under reduced pressure at 50 to 100°C until the viscosity reached 800 to 900 cPs. In this way, the insect oil was recovered. Figure 1 shows the raw material (a), hot air dried material (b), extract (c), primary concentrate (d), secondary concentrate (e), and insect oil (f) in the above process. The ingredients and quality analysis results are shown in Table 3 below.

nutrient Content (% by weight) moisture 87 protein 11 province One batch 3

nutrient Content (% by weight) moisture 9 protein 71 province 5 batch 15

division unit value quality analysis moisture weight% 5 Acid value (AV) mg KOH/g 40 Peroxide value (POV) meq/kg 2.7 heavy metal mg/kg Lead 0.1 or less Inorganic arsenic 0.1 or less Phospholipid content Phospholipids weight% 65 Omega 3 content EPA weight% 8 DHA weight% 5 Fatty acid composition methyl myristate weight% 10.73 Methyl palmitate weight% 22.69 Methyl palmitoleate weight% 4.17 Methyl stearat weight% 1.25 Cis-9-oleic acid methyl ester weight% 10.81 Methyl linoleate weight% 1.88 Methyl linolenate weight% 3.02 Cis-11,14-eicosadienoic acid methyl ester weight% 7.98 EPA weight% 15.12 DHA weight% 8.53 I.S. weight% 14.1

Test Methods

(1) Preparation of test substances

The test substances used in this test are summarized in Table 4 below, and a photo of the substances is shown in Figure 2.

test substance test code name Appearance / Appearance Quantity note snail oil NO (Neomysis awatschensis oil) orange liquid 70g public sample
(Figure 2(a)) garcinia Garcinia white/powder 2g positive sample
(Figure 2(b))

Before using the test substance in the experiment, 100% fermented alcohol was first dissolved in 100% fermented alcohol, then corn oil was added and a stirrer was used. The final volume was prepared using D.W. Each sample was diluted by concentration using D.W, and the prepared sample was stored in a refrigerator.

(2) Experimental animals and breeding environment

During the breeding period, the temperature is 23±1℃, humidity 50±5%, noise less than 60 phones, lighting hours 07:00-19:00 (12 hours a day), illuminance 150-300 lux, ventilation 10-12 times per hour. The environment was maintained. Upon receipt of animals, veterinary quarantine was conducted to check the general health of all animals, and they were allowed to freely consume general rodent feed (Samtoko, Gyeonggi, Korea) and purified water that had been filtered and sterilized with a filter and ultraviolet sterilizer to be suitable for conducting tests. . This experiment was conducted in compliance with the Animal Experiment Ethics Regulations of Invivo Co., Ltd. (Approval No. IV-RA-04-2006-26).

(3) Composition of experimental group

After the acclimatization period was over, high-fat diet and beetle oil were administered for 6 weeks, and obesity was induced and obesity suppressed. The experimental group was the normal group, the control group (obesity-induced group), and the test group by concentration (high-fat diet and insect oil test substance, low concentration (NA 10 mg/kg), high concentration (NA 30 mg/kg). The animals were divided into a positive control group (high-fat diet and 200 mg/kg of Garcinia test substance) and a positive control group (10 animals per group). In the case of the experimental diet, the normal group was fed a regular diet (Sam Taco, Korea), and the obesity-induced model was fed a Rodent Diet with 60%Kcal Fat (Saeron Bio, Korea).

(4) Weekly biomarker analysis and autopsy

Weekly weight change was measured by measuring the weight of each individual in all experimental groups at a certain time once a week, and dietary intake was measured once a week by measuring the remaining amount the day after feeding. For the autopsy, blood was collected after respiratory anesthesia and used for blood analysis, and the liver and epididymal fat were extracted and weighed.

(5) Blood analysis

At the time of animal autopsy, blood was collected from the abdominal vena cava, coagulated for 30 minutes, and then centrifuged at 3,000 rpm for 10 minutes to recover serum. The recovered serum was used for analysis of TC (Total cholesterol), TG (Triglyceride), HDL-C (high density lipoprotein cholesterol), and LDL-C (Low density lipoprotein cholesterol), and was analyzed by the Green Cross Medical Foundation (GC Labs, Korea). The request was made, and the leptin content in the blood was analyzed using an ELISA kit (ab199082; Abcam).

(6) Statistical analysis

Statistical analysis according to the statistical significance test between each group was performed using ANOVA (one-way analysis of variance test) and Duncan's post hoc test comparison, and p < 0.05 was judged to be significant (SPSS V12., SPSS Inc, Chicago, IL, USA).

Test result

(1) Changes in weekly weight and dietary water intake

Figure 3 is a graph showing the results of analyzing the effect of bug oil on the weekly body weight of an obese animal model caused by a high-fat diet.

Referring to FIG. 3, in order to investigate the effect of snail oil on weight gain in an obese animal model induced by a high-fat diet, snail oil of various concentrations was orally administered along with a high-fat diet to induce obesity for 6 weeks and increase body weight. As a result of the investigation, there was a significant difference in body weight between each experimental group from the 4th week of the experiment, with the Normal group being 25.62±0.28 g and the Control group being 28.29±0.74 g, which was significant compared to the normal group. It was found to be relatively high, with the low concentration of impatiens oil (NO10) being 26.65±1.18 g, the high concentration of impregnation oil (NO30) being 27.70±0.96 g, and the experimental control group (Garcinia) being 28.50±0.76 g. Compared to the control group, the low and high concentration administration groups were In that order, the weight gain rate decreased. At the end of the experiment, at week 6, the normal group (Normal) was 26.95 ± 0.28 g and the control group (Control) was 34.01 ± 0.74 g, showing a significant difference in weight gain rate compared to the normal group, and the low concentration of bee oil (NO10) was Body weight was confirmed to be lower in that order: 30.06 ± 1.18 g, high concentration of benign oil (NO30) was 32.15 ± 0.96 g, and 32.71 ± 0.76 g for the experimental control group (Garcinia).

(2) Weekly dietary and water intake

Figure 4 is a graph showing the results of analyzing the effects of python oil on the weekly diet and water intake of an obese animal model caused by a high-fat diet. In Figure 4, A represents dietary intake and B represents drinking water intake.

Referring to FIG. 4, as a result of measuring dietary water intake between each group to confirm the effect of snail oil on the weekly intake and water intake in obesity-induced animals, the dietary intake of each experimental group was normal based on the 6th week. ) was found to be 3.35 ± 0.12 g, the control group was 1.86 ± 0.16 g, the low concentration of gopher oil (10 mg/kg, NO10) was 1.97 ± 0.17 g, and the high concentration of gonorrhea oil (30 mg/kg, NO30) was 1.97 ± 0.17 g. It was 1.87±0.19 g, and the experimental control group (Garcinia) was 1.97±0.15 g. In addition, the drinking water intake as of week 6 was 4.11 ± 0.10 g for the Normal group, 2.15 ± 0.17 g for the Control group, 2.59 ± 0.29 g for low-concentration benix oil (10 mg/kg, NO10), and 2.59 ± 0.29 g for high-concentration benix oil (30 mg/kg, NO10). mg/kg, NO30) was found to be 2.40±0.35 g, and the experimental control group (Garcinia) was found to be 2.46±0.44 g. During the test period, dietary intake and water intake were relatively higher in the normal group consuming a normal diet compared to the control group and experimental group consuming a high-fat diet, but there was no difference between the experimental groups.

(3) Tissue weight

Figure 5 is a graph showing the results of analyzing the effect of bug oil on the tissue weight of an obese animal model. In Figure 5, A represents the liver weight and B represents the epididymal fat weight.

Referring to FIG. 5, as a result of analyzing the effect on liver adipose tissue weight and epididymal fat weight when administered at different concentrations in an obese animal model, liver tissue was first tested to determine the effect on the tissue weight of an obese animal model. In a test measuring the weight, the normal group (Normal) was 1.11 ± 0.02 g and the control group (Control) was 1.15 ± 0.03 g, and it was confirmed that the control group showed a tendency to increase the weight of liver tissue compared to the normal group. On the other hand, the experimental group with low concentration of insect oil (10 mg/kg, NO10) showed 1.00±0.02 g, the experimental group with high concentration of insect oil (30 mg/kg, NO30) showed 1.03±0.03 g, and the experimental control group (Garcinia) showed 1.06±0.02 g. , All experimental groups showed significant differences from the control group. In particular, it was found to be reduced at low and high concentrations of gopher oil.

In the case of epididymal fat tissue, the normal group (Normal) was 0.32 ± 0.02 g, which was significantly increased compared to the control group (1.79 ± 0.13 g). By experimental group, the experimental group with low concentration of insect oil (10 mg/kg, NO10) was 1.36±0.13 g, the experimental group with high concentration of insect oil (30 mg/kg, NO30) was 1.52±0.07 g, and the experimental control group (Garcinia) was 1.76±0.13 g. It was confirmed that the weight of epididymal adipose tissue was reduced in the order of low and high concentrations of insect oil.

(4) General hematological analysis

Figure 6 is a graph showing the results of analyzing the effect of ragweed oil on the concentrations of T-Chol, TG, LDL, and HDL in an obese animal model.

Referring to Figure 6, in order to confirm the effect of dandelion oil on blood T-Chol (Total cholesterol), TG (Triglyceride), LDL (Low-density lipoprotein), and HDL (High-density lipoprotein) concentrations in an obese animal model. As a result of the measurement, the total cholesterol content in the blood was 114.63 ± 2.28 mg/dL in the normal group, which was significantly different from 202.88 ± 9.99 mg/dL in the control group. ) The experimental group showed a similar tendency to the control group at 207.50±7.30 mg/dL and the experimental control group (Garcinia) at 208.63±12.73 mg/dL, but the low concentration of benign oil (10 mg/kg, NO10) experimental group showed 177.88±8.55 mg/dL. showed a tendency to decrease compared to the control group.

As a result of measuring LDL cholesterol in the blood, there was a significant difference between the Normal group (9.89 ± 0.35 mg/dL) and the Control group (36.29 ± 1.83 mg/dL). By experimental group, the low-concentration insect oil (10 mg/kg, NO10) experimental group was 26.80±2.25 mg/dL, the high-concentration insect oil (30 mg/kg, NO30) experimental group was 32.00±1.76 mg/dL, and the control group (Garcinia) experimental group was 34.21. It was found to be ±2.06 mg/dL, and compared to the control group, it was confirmed that the blood LDL content was significantly reduced in the low concentration group in the experimental group administered dandelion oil.

As a result of measuring TG (Triglyceride) in the blood, the control group showed a significant difference of 292.13 ± 9.39 mg/dL compared to the normal group's 185.50 ± 24.23 mg/dL, and the low-concentration benign oil (10 mg/kg, NO10) experimental group showed a significant difference. 273.88±3.89 mg/dL, 281.63±8.70 mg/dL in the high-concentration bean oil (30 mg/kg, NO30) experimental group, and 285.88±4.52 mg/dL in the experimental control (Garcinia) experimental group. There was a significant difference between the experimental groups compared to the control group. No difference appeared.

As a result of measuring HDL cholesterol in the blood, the normal group (Normal) was found to be 103.93 ± 2.46 mg/dL and the control group (Control) was 159.45 ± 6.34 mg/dL. By experimental group, the low-concentration benign oil (10 mg/kg, NO10) experimental group This was 146.10±5.42 mg/dL, the high-concentration garcinia oil (30 mg/kg, NO30) experimental group was 172.81±5.60 mg/dL, and the experimental control group (Garcinia) was 170.45±10.24 mg/dL, compared to the control group. The experimental group administered at a low concentration tended to have lower levels.

(5) Cytokine analysis

Figure 7 is a graph showing the results of analysis of the effect of bug oil on serum leptin concentration in an obese animal model.

It is a cytokine of the Obese (Ob) gene produced in adipose tissue and is a hormone that suppresses appetite and causes a feeling of fullness. It regulates appetite and energy metabolism. It is known that leptin secretion increases in the case of obesity, so it is often used in obesity research as an indicator of body fat mass. Referring to Figure 7, the amount of serum leptin was measured and analyzed to confirm the anti-obesity effect of gnatia oil in an obese animal model. As a result, the amount was 535.3 ± 108.8 pg/ml in the normal group, compared to 5088.7 ± 476.8 in the control group. It was significantly lower than pg/ml. On the other hand, the experimental group with low concentration of insect oil (10 mg/kg, NO10) was 3629.9±624.8 pg/ml, the experimental group with high concentration of insect oil (30 mg/kg, NO30) was 4043.7±525.4 pg/ml, and the experimental control group (Garcinia) was 5176.8±. It was found to be 406.6 pg/ml, and it was confirmed that the concentration of leptin in the blood tended to decrease or was significantly decreased in the experimental group administered dandelion oil compared to the control group.

In this way, it has been confirmed that gnatia oil has excellent anti-obesity efficacy, with significant results in weight loss, reduction in liver adipose tissue and epididymal fat weight, and reduction in blood cholesterol, LDL, and HDL contents.

Hereinafter, a manufacturing example of the application of the anti-obesity food composition containing the insect oil according to the present invention will be described, but it is not limited thereto.

Preparation Example 1: Powder preparation

Table 5 below illustrates the powder composition of the anti-obesity food composition containing the insect oil according to the present invention described above. Powder can be prepared by mixing the following ingredients and filling them in an airtight bubble.

ingredient content snail oil 200 mg lactose 100 mg Talc 10 mg

Preparation Example 2: Tablet Preparation

Table 6 below illustrates the tablet composition of the anti-obesity food composition containing the insect oil according to the present invention described above. Tablets can be manufactured by mixing the following ingredients and compressing them according to a conventional tablet manufacturing method.

ingredient content snail oil 200 mg corn starch 100 mg lactose 100 mg Magnesium Stearate 2mg

Preparation Example 3: Capsule preparation

Table 7 below illustrates the capsule composition of the anti-obesity food composition containing the insect oil according to the present invention described above. Capsules can be manufactured by mixing the following ingredients and filling them into gelatin capsules according to a typical capsule manufacturing method.

ingredient content snail oil 200 mg crystalline cellulose 3mg lactose 14.8 mg Magnesium stearate 0.2 mg

Preparation Example 4: Liquid preparation

Table 8 below illustrates the liquid composition of the anti-obesity food composition containing the insect oil according to the present invention described above. The liquid can be prepared by adding and dissolving each of the following ingredients in purified water according to a typical liquid preparation method, adding an appropriate amount of lemon flavor, mixing the following ingredients, adding purified water to adjust the total to 100 ml, and then filling and sterilizing it in a brown bottle. You can.

ingredient content snail oil 200 mg Iseonghwadang 10g mannitol 5g Purified water Appropriate amount

Preparation Example 5: Preparation of health food

Table 9 below shows the functional health food composition of the anti-obesity food composition containing the insect oil according to the present invention described above. In the composition below, the composition ratio of the vitamin and mineral mixture represents a mixture of ingredients relatively suitable for health food as a preferred preparation example, but the mixing ratio may be modified arbitrarily, and each component is mixed according to a typical health food manufacturing method. Then, granules can be prepared and used to prepare health food compositions according to conventional methods.

ingredient content ingredient content snail oil 1000 mg folic acid 50 μg vitamin mixture Appropriate amount Calcium Pantothenate 0.5 mg Vitamin A Acetate 70 ㎍ mineral mixture Appropriate amount Vitamin E 1.0 mg Ferrous sulfate 1.75 mg Vitamin B1 0.13 mg zinc oxide 0.82 mg Vitamin B2 0.15 mg Magnesium Carbonate 25.3 mg Vitamin B6 0.5 mg Potassium Phosphate Monobasic 15 mg Vitamin B12 0.2 μg potassium phosphate dibasic 55 mg Vitamin C 10 mg potassium citrate 90 mg biotin 10 μg calcium carbonate 100 mg Nicotinic acid amide 1.7 mg Magnesium chloride 24.8 mg

Preparation Example 6: Preparation of health drink

Table 10 below shows the functional health drink composition of the anti-obesity food composition containing the insect oil according to the present invention described above. The production of a health drink follows a typical health drink production method, for example, mixing the following ingredients, stirring and heating at 85°C for about 1 hour, filtering the resulting solution, placing it in a sterilized 2 liter container, sealing and sterilizing it. It can be stored refrigerated and used. The following composition ratio is a mixture of ingredients that are relatively suitable for beverages of choice as a preferred example, but the mixing ratio may be arbitrarily modified depending on regional and ethnic preferences such as demand class, demand country, and usage purpose.

ingredient content snail oil 1000 mg citric acid 1000 mg oligosaccharide 100g plum concentrate 2g taurine 1g Purified Water Mix Total 900 ml

The preferred embodiments of the present invention described above were disclosed to solve technical problems, and those skilled in the art will be able to make various modifications, changes, additions, etc. within the spirit and scope of the present invention. , such modifications and changes should be regarded as falling within the scope of the patent claims below.

Claims (3)

(a) extracting 90 to 100% (v/v) alcohol by adding 10 to 20 times the amount of alcohol to the dried insect;
(b) first filtering the extract of step (a) and then first concentrating it under reduced pressure at 40 to 60°C; and
(c) secondary filtration of the concentrate from step (b) and secondary concentration under reduced pressure at 50 to 100°C to produce an oil with a viscosity of 700 to 1,000 cPs;
As a method for producing anti-obesity insect oil comprising,
The bug oil is an active ingredient that exhibits anti-obesity efficacy confirmed through weight loss, reduction in liver adipose tissue and epididymal fat weight, and reduction in blood leptin concentration in an obesity-inducing animal model,
A method for producing anti-obesity chamomile oil, characterized in that the phospholipid content of the chamomile oil is 60 to 70% by weight. delete delete