KR102613067B1 - Amot 및 wdr76를 검출하기 위한 프라이머를 포함하는 세포 노화를 검출하기 위한 조성물 - Google Patents
Amot 및 wdr76를 검출하기 위한 프라이머를 포함하는 세포 노화를 검출하기 위한 조성물 Download PDFInfo
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Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
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| KR1020210159264 | 2021-11-18 | ||
| KR20210159264 | 2021-11-18 |
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| KR20230074026A KR20230074026A (ko) | 2023-05-26 |
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| KR1020220155749A KR102613067B1 (ko) | 2021-11-18 | 2022-11-18 | Amot 및 wdr76를 검출하기 위한 프라이머를 포함하는 세포 노화를 검출하기 위한 조성물 |
| KR1020220155838A KR102639826B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 gas2l3 및 amot의 rt-pcr 검출을 이용한 세포 노화를 검출하는 방법 |
| KR1020220155841A KR102657184B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 세포노화 바이오 마커를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155847A KR102640975B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3, amot를 검출하기 위한 제제를 포함하는 세포 노화 검출 키트 |
| KR1020220155753A KR102639822B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 amot 및 wdr76를 검출하기 위한 rt-pcr 키트 |
| KR1020220155843A KR102657186B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 세포노화 바이오 마커 gas2l3 및 amot를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155745A KR102639818B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3, amot를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155756A KR20230074033A (ko) | 2021-11-18 | 2022-11-18 | 기계 학습을 이용한 세포 노화의 마커를 선별하는 방법, 세포 노화 바이오 마커, 및 이를 이용한 세놀리틱 제제를 스크리닝 하는 방법 |
| KR1020220155754A KR102639823B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 gas2l3 및 wdr76를 검출하기 위한 rt-pcr 키트 |
| KR1020220155744A KR102639817B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155850A KR102657188B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 프라이머를 이용하여 세포노화 바이오 마커를 검출하는 방법 |
| KR1020220155842A KR102657185B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 세포노화 바이오 마커 gas2l3 및 wdr76를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155746A KR102639819B1 (ko) | 2021-11-18 | 2022-11-18 | Amot, wdr76를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155839A KR102639827B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 gas2l3 및 wdr76의 rt-pcr 검출을 이용한 세포 노화를 검출하는 방법 |
| KR1020220155755A KR102639824B1 (ko) | 2021-11-18 | 2022-11-18 | Rt-pcr을 이용한 세포 노화를 검출하는 방법 |
| KR1020220155848A KR102640976B1 (ko) | 2021-11-18 | 2022-11-18 | Amot, wdr76를 검출하기 위한 제제를 포함하는 세포 노화 검출 키트 |
| KR1020220155751A KR102639820B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화를 검출하기 위한 rt-pcr 키트 |
| KR1020220155752A KR102639821B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 gas2l3 및 amot를 검출하기 위한 rt-pcr 키트 |
| KR1020220155844A KR102657187B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 세포노화 바이오 마커 amot 및 wdr76를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155849A KR102640977B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3, wdr76를 검출하기 위한 제제를 포함하는 세포 노화 검출 키트 |
| KR1020220155748A KR102613066B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3 및 amot를 검출하기 위한 프라이머를 포함하는 세포 노화를 검출하기 위한 조성물 |
| KR1020220155846A KR102640974B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커를 검출하기 위한 제제를 포함하는 세포 노화 검출 키트 |
| KR1020220155750A KR102614667B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3 및 wdr76를 검출하기 위한 프라이머를 포함하는 세포 노화를 검출하기 위한 조성물 |
| KR1020220155845A KR102613068B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커 gas2l3, wdr76, 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR1020220155852A KR102657190B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 프라이머를 이용하여 세포노화 바이오 마커 gas2l3 및 amot를 검출하는 방법 |
| KR1020220155742A KR102614665B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커 gas2l3, amot, 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR1020220155741A KR102614664B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR1020220155840A KR102639828B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 amot 및 wdr76의 rt-pcr 검출을 이용한 세포 노화를 검출하는 방법 |
| KR1020220155837A KR102639825B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3 및 wdr76를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155743A KR102614666B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커 amot, wdr76 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR1020220155851A KR102657189B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 프라이머를 이용하여 세포노화 바이오 마커 gas2l3 및 wdr76를 검출하는 방법 |
| KR1020220155747A KR102613065B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커를 검출하기 위한 프라이머를 포함하는 세포 노화를 검출하기 위한 조성물 |
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| KR1020220155838A KR102639826B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 gas2l3 및 amot의 rt-pcr 검출을 이용한 세포 노화를 검출하는 방법 |
| KR1020220155841A KR102657184B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 세포노화 바이오 마커를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155847A KR102640975B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3, amot를 검출하기 위한 제제를 포함하는 세포 노화 검출 키트 |
| KR1020220155753A KR102639822B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 amot 및 wdr76를 검출하기 위한 rt-pcr 키트 |
| KR1020220155843A KR102657186B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 세포노화 바이오 마커 gas2l3 및 amot를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155745A KR102639818B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3, amot를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155756A KR20230074033A (ko) | 2021-11-18 | 2022-11-18 | 기계 학습을 이용한 세포 노화의 마커를 선별하는 방법, 세포 노화 바이오 마커, 및 이를 이용한 세놀리틱 제제를 스크리닝 하는 방법 |
| KR1020220155754A KR102639823B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 gas2l3 및 wdr76를 검출하기 위한 rt-pcr 키트 |
| KR1020220155744A KR102639817B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155850A KR102657188B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 프라이머를 이용하여 세포노화 바이오 마커를 검출하는 방법 |
| KR1020220155842A KR102657185B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 세포노화 바이오 마커 gas2l3 및 wdr76를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155746A KR102639819B1 (ko) | 2021-11-18 | 2022-11-18 | Amot, wdr76를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155839A KR102639827B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 gas2l3 및 wdr76의 rt-pcr 검출을 이용한 세포 노화를 검출하는 방법 |
| KR1020220155755A KR102639824B1 (ko) | 2021-11-18 | 2022-11-18 | Rt-pcr을 이용한 세포 노화를 검출하는 방법 |
| KR1020220155848A KR102640976B1 (ko) | 2021-11-18 | 2022-11-18 | Amot, wdr76를 검출하기 위한 제제를 포함하는 세포 노화 검출 키트 |
| KR1020220155751A KR102639820B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화를 검출하기 위한 rt-pcr 키트 |
| KR1020220155752A KR102639821B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 gas2l3 및 amot를 검출하기 위한 rt-pcr 키트 |
| KR1020220155844A KR102657187B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 세포노화 바이오 마커 amot 및 wdr76를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155849A KR102640977B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3, wdr76를 검출하기 위한 제제를 포함하는 세포 노화 검출 키트 |
| KR1020220155748A KR102613066B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3 및 amot를 검출하기 위한 프라이머를 포함하는 세포 노화를 검출하기 위한 조성물 |
| KR1020220155846A KR102640974B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커를 검출하기 위한 제제를 포함하는 세포 노화 검출 키트 |
| KR1020220155750A KR102614667B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3 및 wdr76를 검출하기 위한 프라이머를 포함하는 세포 노화를 검출하기 위한 조성물 |
| KR1020220155845A KR102613068B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커 gas2l3, wdr76, 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR1020220155852A KR102657190B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 프라이머를 이용하여 세포노화 바이오 마커 gas2l3 및 amot를 검출하는 방법 |
| KR1020220155742A KR102614665B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커 gas2l3, amot, 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR1020220155741A KR102614664B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR1020220155840A KR102639828B1 (ko) | 2021-11-18 | 2022-11-18 | 세포노화 바이오 마커 amot 및 wdr76의 rt-pcr 검출을 이용한 세포 노화를 검출하는 방법 |
| KR1020220155837A KR102639825B1 (ko) | 2021-11-18 | 2022-11-18 | Gas2l3 및 wdr76를 이용한 세포 노화를 검출하는 방법 |
| KR1020220155743A KR102614666B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커 amot, wdr76 및 이를 이용한 세포 노화를 검출하기 위한 조성물 |
| KR1020220155851A KR102657189B1 (ko) | 2021-11-18 | 2022-11-18 | 분리된 세포로부터 프라이머를 이용하여 세포노화 바이오 마커 gas2l3 및 wdr76를 검출하는 방법 |
| KR1020220155747A KR102613065B1 (ko) | 2021-11-18 | 2022-11-18 | 세포 노화 바이오 마커를 검출하기 위한 프라이머를 포함하는 세포 노화를 검출하기 위한 조성물 |
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| WO (1) | WO2023090901A1 (fr) |
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| WO2014075083A1 (fr) | 2012-11-09 | 2014-05-15 | The Regents Of The University Of California | Procédés de pronostic de l'âge et agents d'identification qui induisent ou inhibent le vieillissement prématuré |
Family Cites Families (17)
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| KR100734429B1 (ko) * | 2004-12-06 | 2007-07-03 | 재단법인서울대학교산학협력재단 | 노화에 관여하는 신호 및 분자종 |
| WO2006081494A2 (fr) * | 2005-01-28 | 2006-08-03 | Ordway Research Institute, Inc. | Induction de la senescence des cellules tumorales par des agonistes et des antagonistes du recepteur retinoide |
| US7908090B2 (en) | 2005-11-30 | 2011-03-15 | The Board Of Trustees Of The Leland Stanford Junior University | Signatures for human aging |
| EP3257943B1 (fr) * | 2010-11-02 | 2019-09-11 | Helmholtz-Zentrum für Infektionsforschung GmbH | Procédés et vecteurs pour l'immortalisation de cellules |
| EP2678792B1 (fr) * | 2011-02-22 | 2020-11-04 | The Procter and Gamble Company | Procédés d'identification d'agents cosmétiques pour des compositions de soins de la peau |
| RU2016138847A (ru) | 2014-04-07 | 2018-05-07 | Хэлф Ресерч, Инк. | Композиции и способы, относящиеся к покоящимся предрасположенным к старению клеткам (DSPC) |
| WO2016118859A1 (fr) | 2015-01-22 | 2016-07-28 | Board Of Trustees Of The University Of Arkansas | Compositions et procédés de déplétion sélective de cellules sénescentes comprenant la protéine flip |
| IL255920B2 (en) * | 2015-05-28 | 2024-05-01 | Celularity Inc | Stem cells derived from the placenta and their use for the restoration of the regeneration engine, for the repair of protein defects for the extension of the useful life of aging items |
| CN110268263B (zh) * | 2016-11-25 | 2022-07-19 | 阿尔卑斯格勒诺布尔大学 | 人体皮肤老化的新生物标志物 |
| US10665326B2 (en) | 2017-07-25 | 2020-05-26 | Insilico Medicine Ip Limited | Deep proteome markers of human biological aging and methods of determining a biological aging clock |
| US20200286625A1 (en) | 2017-07-25 | 2020-09-10 | Insilico Medicine Ip Limited | Biological data signatures of aging and methods of determining a biological aging clock |
| JP7462569B2 (ja) | 2017-12-12 | 2024-04-05 | クィーンズ ユニバーシティー アット キングストン | Ｃｙｐ２６酵素を阻害するための化合物および方法 |
| GB201721738D0 (en) * | 2017-12-22 | 2018-02-07 | King S College London | Method |
| KR102291649B1 (ko) * | 2018-03-23 | 2021-08-20 | 의료법인 성광의료재단 | 줄기세포의 노화 예측 또는 진단용 바이오 마커 |
| WO2020047473A1 (fr) | 2018-08-30 | 2020-03-05 | HCW Biologics, Inc. | Polypeptides chimères à chaîne unique et à chaînes multiples et leurs utilisations |
| PE20211006A1 (es) * | 2018-10-02 | 2021-06-01 | Lunella Biotech Inc | Derivados de azitromicina y roxitromicina como farmacos senoliticos |
| EP3643305A1 (fr) * | 2018-10-25 | 2020-04-29 | Universität für Bodenkultur Wien | Compositions pour l'élimination de cellules sénescentes |
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