KR102562050B1 - A Gami-Hwangryunhaedoktang hand sanitizer composition with anti-bacterial and moisturizing effect - Google Patents

Abstract

The present invention relates to a hand sanitizer composition comprising Gamihwangryeonhaedoktang having antibacterial and moisturizing effects as an active ingredient. The composition according to the present invention has antibacterial and moisturizing effects against Escherichia coli, Staphylococcus aureus and Phi6 virus.

Description

A hand sanitizer composition containing Gamihwangryunhaedoktang hand sanitizer composition with anti-bacterial and moisturizing effect}

The present invention relates to a hand sanitizer composition comprising Gamihwangryeonhaedoktang having antibacterial and moisturizing effects as an active ingredient. The composition according to the present invention has antibacterial and moisturizing effects against Escherichia coli, Staphylococcus aureus and Phi6 virus.

Hwangnyeon Haedok-tang is a decoction made by mixing 5g each of Hwangryeon, Hwanggeum, Hwangbaek and Gardenia per 1 serving. It is stuffy and sad, dry mouth and throat, high fever, nonsense, inability to sleep, nosebleeds, bleeding spots, and severe symptoms of endotoxin when various boils are used. It can be used in various cases of acute inflammation, sepsis, acute pneumonia, high blood pressure, and rosacea.

Addition (加味) refers to adjusting the weight ratio of the medicines written in the original drugstore according to the condition of the patient when preparing medicine, removing medicines, or adding other medicines. Accordingly, the efficacy of the existing prescription may be enhanced or a new efficacy may be added. Therefore, Kami prescription uses the name of an existing prescription in that it uses the existing prescription's medicinal ingredients, but in reality it can be said to be a new prescription.

The inventor of the present application devised a disinfectant for Gamihwangnyeon Haedok-tang, which is based on Hwangnyeon Haedok-tang but has antibacterial and moisturizing effects in order to be used as a disinfectant. In other words, in addition to gold, yellow lotus, yellow white, and gardenia, gold silver flower, yeongyo, clove, and octagonal fennel were added to create an active ingredient for disinfection.

Looking at the prior art literature, Korean Registered Patent Publication No. 10-2199934 describes "a cosmetic composition for improving acne containing fermented product of kimchi lactic acid bacteria of Hwangnyeon Haedok-tang". Looking at it, it is described in claim 1 to include the fermented product of Hwangryeonhaedoktang lactic acid bacteria fermented with Lactobacillus plantarum strain as an active ingredient. In addition, Korean Patent Registration No. 10-1819060 describes a "cosmetic composition containing fermented product of Hwangnyeon Haedok-tang as an active ingredient". Looking at it, it is described in claim 1 to include the fermented product of Hwangnyeonhaedoktang fermented with Leuconostoc mesenteroides as an active ingredient.

In summary, the prior art documents are similar to the invention of this application in that they are external preparations based on Hwangnyeon Haedok-tang, but do not contain other materials used in the invention of this application, and the functionality as a hand sanitizer cannot be grasped. .

Republic of Korea Registered Patent Publication No. 10-2199934 (registered on 2021.01.04) Republic of Korea Registered Patent Publication No. 10-1819060 (registered on 2018.01.10)

The problem to be solved is to devise Gamihwangryeonhaedoktang having antibacterial and moisturizing effects for use as a hand sanitizer and to provide a hand sanitizer composition containing it as an active ingredient.

The solution is a hand sanitizer composition containing a mixed extract of gold, yellow lotus, yellow white, gardenia, gold silver flower, lotus root, clove and octagonal fennel as an active ingredient.

In the mixed extract, gold, yellow lotus, yellow white, gardenia, gold silver flower, yeongyo, cloves and octagonal fennel are mixed in the same weight.

The composition is characterized by having antibacterial and moisturizing effects against Escherichia coli, Staphylococcus aureus and Phi6 virus.

The solution is a hand sanitizer comprising a hand sanitizer composition containing a mixed extract of golden, yellow lotus, yellow white, gardenia, gold silver flower, lotus root, clove and octagonal fennel as an active ingredient.

The composition according to the present invention has antibacterial and moisturizing effects against Escherichia coli, Staphylococcus aureus and Phi6 virus.

1 to 6 are graphs and photographs showing experimental results according to Experimental Example 1.

Features and advantages of the present invention will be more clearly understood by the experimental examples and examples described by the accompanying drawings.

The application of the present invention is not limited by the configuration and arrangement of components described in the experimental examples and examples or shown in the drawings. The invention is capable of implementation in other embodiments and of being carried out in various ways. In addition, expressions and predicates used in the embodiments with respect to terms such as the orientation of devices or elements are merely used to simplify the description of the present invention, and do not indicate or imply that related devices or elements should simply have a specific orientation. . For example, when terms such as "first" and "second" are used in the embodiments and claims describing the present invention, these terms are not intended to indicate or imply a relative significance or meaning.

In addition, the terms or words used in this specification and claims should not be construed as being limited to a conventional or dictionary meaning, and the inventors should not use the technical spirit of the present invention to explain the terms and concepts of the invention in the best way. It should be interpreted as written based on the corresponding meaning and concept.

Therefore, the present invention is not limited to the presented embodiments, and the equivalent scope of the technical idea of the present invention and the technical idea described in the claims to be described below by those skilled in the art to which the present invention belongs Various modifications and changes are possible within.

Preparation Example 1. Gami Hwangryun Haedoktang Active Ingredient Sample Preparation

The active ingredients of Gamihwangryeonhaedok-tang according to the present invention include golden, yellow lotus, yellow white, gardenia, gold silver flower, yeonkyo, clove and octagonal fennel. Table 1 below summarizes the substances included in the materials included in the active ingredient and their efficacy.

natural product part natural active ingredient efficacy Gold
(Scutellariae Radix) golden root Baicalin, Baicalein, Woogonin, Woogonoside, Neobaicalein, β-Sistosterol. detoxification of fire,
Antipyretic, antibacterial,
Antivirus, antifungal, soothing Coptis Rhizoma rhizome root Coptisine, Palmatine, Berberine Sterilization, insecticide, anti-inflammatory yellow white
(Phellodendri Cortex) Yellowish Bark Berberine, Obaculactone, Obacunone, Dictamnolactone. Antibacterial, antifungal, anti-inflammatory, subcutaneous bleeding absorption promotion, anti-inflammatory Gardenia
(Gardeniae fructus) gardenia fruit Genipin, Geniposidic acid, Chlorogenic acid, Geniposide Anti-inflammatory, antioxidant, atopy gold and silver coins
(Caprifoliaceae) honeysuckle flower Luteolin, Iinositol, Saponin, Tannin Antibacterial action, anti-inflammatory effect, immune enhancing action annual school
(Oleaceae) Uiseong Forsythia
Fruit Oil Forsythol, Oleanic acid, Vitamin P Detoxification, anti-inflammatory, allergic asthma cloves
(Saururaceae) flower buds of cloves Decanoyl acetaldehyde, Methyl nonylketone, α-Pinene, Myrcene, Limonene, p-Cresol, Quercetin, Cl,K2SO4. Clear heat detoxification, antibacterial, antiviral, anti-inflammatory. octagonal fennel
(Boraginaceae) fruit of octagonal fennel Acetylshikonin, β-Dimethylcrylshikonin, Isobutylshikonin. Clearing blood, clearing heat, detoxifying, antibacterial, antifungal.

Eight of the above materials were formulated in the same weight ratio (1:1) and distilled and extracted for 3 hours. This distilled extract was used as a sample. (Sample concentration 100ul/mL, applied to Experimental Example 1)

Experimental Example 1. Antibacterial, Phi6 antiviral and moisture analysis

[Test method]

1) Analysis of antibacterial activity: E. coli ATCC10536 and S. aureus ATCC6538 strains were pre-cultured in LB liquid medium, then inoculated at 1/100 into fresh 5 m LB liquid medium and cultured with shaking at 37°C for 16 hours. The cultured bacterial solution was used as a stock solution or diluted 1/10 as a bacterial dilution. Antibacterial ability analysis was performed by slightly modifying the neutralization dilution test method among the sterilization and disinfection test methods of the Ministry of Food and Drug Safety.

2) Phi6 sample preparation

Bacteriophage phi6 was infected with Pseudomonas sp, and the supernatant was collected by centrifugation. The collected supernatant was sterilized with a 0.2 um filter. Confirmation of Phi6 was determined by whether or not phage plaques were formed after mixing with chloroform. Phi6 stock maintained ~1010 PFU (plaque forming unit)/ml.

3) Analysis of antiviral ability

After mixing the same amount of Gamihwangryeonhaedoktang sample with phi6, it was reacted at 20℃ for 5 to 30 minutes. The reactants were serially diluted 10-fold, and a certain amount was dropped onto the agar medium already hardened with a lawn on Top agar, and then incubated at 25 ° C. After observing Phi6 plaques for 24-48 hours of culture, antivirus activity was analyzed.

[Experiment result]

1) Antibacterial ability analysis

As a result of the test according to the neutralization dilution method of the Ministry of Food and Drug Safety, the detection level of 103 CFU / ml or less was found to be reduced in the disinfectant of Gamihwangryeon Haedok-tang. This result is estimated to have at least 99.999% or more antibacterial activity. (See Fig. 1, viable cell count)

2) Antibacterial analysis of Escherichia coli and Staphylococcus aureus

Looking at the photo Figure 2 of OH before sample input and the photo Figure 3 after 5MIN after sample input, the antibacterial ability against E. coli can be confirmed. In addition, looking at the photo Figure 4 of OH before sample input and the photo Figure 5 after 5MIN after sample input, the antibacterial ability against Staphylococcus aureus can be confirmed. (E. coli, Staphylococcus aureus 8.8x10 ⁶ CFU/mL)

3) Phi6 antiviral activity assay

Phi6 virus is an RNA virus surrounded by lipid components, such as influenza and coronavirus, and is used as an infection research model for influenza, SARS, MERS, and coronavirus.

As shown in FIG. 6, the phi6 antiviral effect of the Gamihwangryeon Haedok-tang disinfectant showed an activity of 99.991% or more when treated for 5 minutes or more, and an activity of 99.999% or more when treated for 30 minutes or more. (See Figure 6, the number of infected phages)

4) Moisturizing effect analysis

As an improvement effect on dryness, which is the main cause of atopic skin, a moisturizing effect experiment was conducted for each extract. Completely dried CaCl 2 was placed in a desiccator using a desiccant, and 4 g of each of the extracts was weighed on a dish, and each sample was weighed three times for 12 hours in a desiccator at 50% relative humidity and 35 ° C. As a result, it was found that Gamihwangryeonhaedoktang disinfectant had excellent moisturizing effect.

raw material name Moisturizing effect experiment 0hr 3hr 12hr 70% alcohol disinfectant 100% 60% 29% Sample of Gami Hwangryun Haedoktang 100% 80% 54%

Example 1. Hand sanitizer composition containing Gamihwangryeonhaedoktang having antibacterial and moisturizing effects as an active ingredient

It contains Gamihwangryeonhaedoktang described in Preparation Example 1 according to the present invention as an active ingredient. The composition according to the present invention may contain alcohol in addition to the active ingredient. In addition, it may further include a pharmaceutically acceptable substance that can be used in hand sanitizer.

So far, the present invention has been mainly looked at with respect to preferred embodiments.

The embodiments described in this specification and the configurations shown in the drawings relate to one of the most preferred embodiments of the present invention, and do not represent all of the technical spirit of the present invention, so various equivalents and modifications that can replace them It should be understood that there may be examples.

Therefore, the present invention is not limited to the presented embodiments, and within the equivalent scope of the technical idea of the present invention and the technical idea described in the claims to be described below by those skilled in the art to which the present invention belongs There may be embodiments in which various modifications and changes are possible.

Claims (4)

A composition containing a mixed extract of gold, yellow lotus, yellow white, gardenia, gold silver flower, lotus root, clove and octagonal fennel as an active ingredient,
The mixed extract is a mixed extract extracted by distillation by mixing each material in the same weight,
The concentration of the mixed extract is 100ul/mL,
The mixed extract is characterized in that it has antibacterial efficacy against Escherichia coli, Staphylococcus aureus and Phi6 virus, and moisturizing effect.
Hand sanitizer composition.
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