KR102556067B1 - Method of manufacturing extracted composition from baby watermelon for diminishing edema and food composition for diminishing edema - Google Patents
Method of manufacturing extracted composition from baby watermelon for diminishing edema and food composition for diminishing edema Download PDFInfo
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- 241000219109 Citrullus Species 0.000 title claims abstract description 104
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 title claims abstract description 103
- 206010030113 Oedema Diseases 0.000 title claims abstract description 47
- 239000000203 mixture Substances 0.000 title claims abstract description 29
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 5
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- 230000003467 diminishing effect Effects 0.000 title 2
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
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- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 8
- 239000011707 mineral Substances 0.000 claims description 8
- 239000000679 carrageenan Substances 0.000 claims description 7
- 229940113118 carrageenan Drugs 0.000 claims description 7
- 235000010418 carrageenan Nutrition 0.000 claims description 7
- 229920001525 carrageenan Polymers 0.000 claims description 7
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 claims description 7
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 4
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/40—Table salts; Dietetic salt substitutes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
- A23L3/44—Freeze-drying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/51—Concentration
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/10—Drying, dehydrating
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
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- Health & Medical Sciences (AREA)
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- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Botany (AREA)
- Inorganic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
본 발명은 애기수박을 수거하는 단계; 상기 애기수박을 절편화하여 건조하는 단계; 상기 건조된 애기수박 절편을 열수 추출하여 추출액을 준비하는 단계; 상기 추출된 추출액을 농축 및 동결건조하여 애기수박 추출 분말을 수득하는 단계를 포함하는 부종완화용 애기수박 추출 조성물의 제조방법을 제공한다. 아울러, 상기 애기수박 추출 분말 또는 애기수박 추출물 소금을 포함함으로써 부종완화 효과를 갖는 기능성 식품 조성물을 제공한다. The present invention includes the steps of collecting baby watermelon; Slicing and drying the baby watermelon; Preparing an extract by hot water extraction of the dried baby watermelon slices; It provides a method for producing a baby watermelon extract composition for edema relief comprising the steps of concentrating and freeze-drying the extracted extract to obtain a baby watermelon extract powder. In addition, a functional food composition having an edema relieving effect is provided by including the baby watermelon extract powder or the baby watermelon extract salt.
Description
본 발명은 추출물 분야의 기술로서, 구체적으로는 신규의 효능을 갖는 애기수박 추출물의 추출 방법과 활용에 관한 기술이다. The present invention is a technology in the field of extracts, and specifically, a method for extracting and utilizing an extract of Arabidopsis watermelon having novel efficacy.
2016년 통계청 자료에 따르면 2016년 한해 수박 생산량은 635,000 톤이며, 수박은 사과, 포도, 귤, 복숭아와 함께 국내 5대 소비 과일중 하나이다. According to data from the National Statistical Office in 2016, watermelon production in 2016 was 635,000 tons, and watermelon is one of the top five consumed fruits in Korea along with apples, grapes, tangerines, and peaches.
수박 재배농가에서 상품성 수박(대과, 어른수박)을 생산하기 위해 덩굴치기(적과) 과정은 필수 재배법으로서, 이러한 적과 과정에서 발생한 애기수박은 연간 3만 통 이상으로 알려져 있다. 이러한 애기수박의 발생율에 따르면 국내 수박 농가 1가구당 2톤에 해당하는 애기수박이 폐기되고 있는 실정이다. In order to produce commercial watermelons (large fruits and adult watermelons) in watermelon growers, the vine pruning (red fruit) process is an essential cultivation method, and it is known that more than 30,000 baby watermelons generated in this fruit process are produced annually. According to the incidence rate of baby watermelon, 2 tons of baby watermelon per household in domestic watermelon farms is being discarded.
현재까지 수박 재배 농가에서는 애기수박 폐기와 관련된 효율적인 방안이 없는 상황이며, 국내외 여러 산업군에서도 애기수박을 활용하여 신규 기능성 소재로 전환하여 근본적인 문제점을 해결한 사례가 알려져 있지 않다. Until now, watermelon growers do not have an efficient plan related to the disposal of baby watermelon, and there are no known cases of solving fundamental problems by using baby watermelon in various industries at home and abroad by converting it into a new functional material.
본 발명은 전순한 바와 같이, 아직 해결되지 않은 애기수박 폐기물의 활용 방안을 제시하고자 도출된 발명으로서, 부종을 효율적으로 억제하고 완화할 수 있는 애기수박 추출 조성물의 제조방법을 제공하는 것을 목적으로 한다. As described above, the present invention is an invention derived to suggest a utilization method of baby watermelon waste that has not yet been solved, and aims to provide a method for producing a baby watermelon extract composition that can efficiently suppress and alleviate edema. .
본 발명은 또한, 상기 방법에 의하여 제조된 추출 조성물을 다양한 방법으로 식품에 도입한 부종완화용 식품 조성물을 제공하는 것을 목적으로 한다. An object of the present invention is also to provide a food composition for edema relief by introducing the extract composition prepared by the above method into food by various methods.
본 발명의 일 실시예에 따른 부종완화용 애기수박 추출 조성물의 제조방법은 애기수박을 수거하는 단계; 상기 애기수박을 절편화하여 건조하는 단계; 상기 건조된 애기수박 절편을 열수 추출하여 추출액을 준비하는 단계; 상기 추출된 추출액을 농축 및 동결건조하여 애기수박 추출 분말을 수득하는 단계를 포함한다. A method for preparing a baby watermelon extract composition for edema relief according to an embodiment of the present invention includes collecting baby watermelon; Slicing and drying the baby watermelon; Preparing an extract by hot water extraction of the dried baby watermelon slices; Concentrating and freeze-drying the extracted extract to obtain an extract powder of baby watermelon.
상기 방법은 상기 추출액을 반복적으로 증발시켜 추출액내 고형분인 애기수박 추출물 소금을 준비하는 단계를 추가적으로 포함함으로써, 애기수박 추출물 소금을 별도로 분리해낼 수 있다. The method further includes the step of repeatedly evaporating the extract to prepare the baby watermelon extract salt, which is a solid in the extract, so that the baby watermelon extract salt can be separated separately.
상기 열수 추출은 미네랄 함유 증류수를 추출용매로 하여 이루어질 수 있다. 상기 미네랄 함유 증류수는, 증류수에, 현무암을 800 내지 1000℃에서 소성한 후 냉각하여 분말화된 현무암 유래 미네랄을 첨가하고 1 내지 2주간 방치한 후, 분말 성분을 여과한 후 수득될 수 있다. The hot water extraction may be performed using mineral-containing distilled water as an extraction solvent. The mineral-containing distilled water may be obtained after calcining basalt at 800 to 1000 ° C., adding powdered basalt-derived minerals to distilled water, leaving it for 1 to 2 weeks, and filtering the powder component.
본 발명의 일 실시예에 따른 부종완화용 식품 조성물은 전술한 방법으로 제조된 애기수박 추출 분말 또는 전술한 애기수박 추출물 소금; 및 식품학적으로 허용가능한 식품 첨가물을 포함한다. A food composition for edema relief according to an embodiment of the present invention includes the above-mentioned baby watermelon extract powder or the above-mentioned baby watermelon extract salt; and phytochemically acceptable food additives.
상기 애기수박 주출 분말 및 애기수박 추출물 소금은, 카라기닌(carrageenan)에 의하여 부종이 유발된 쥐(mice)에 투여하고 60분 경과 후, 대조군의 83.2 ± 0.8% 부종 증가율 대비, 각각 37.2 ± 1.7% 및 35.3 ± 5.0%의 부종 증가율을 나타낼 수 있다. The baby watermelon extract powder and the baby watermelon extract salt were administered to mice whose edema was induced by carrageenan, and after 60 minutes, 37.2 ± 1.7%, respectively, compared to the 83.2 ± 0.8% edema increase rate of the control group. and an edema increase rate of 35.3 ± 5.0%.
본 발명의 다른 실시예에 따른 부종완화용 식품 조성물은 전술한 애기수박 추출물 소금이, 식품 조성물 내의 나트륨 성분을 대체하여 첨가된 식품 조성물일 수 있다. A food composition for edema relief according to another embodiment of the present invention may be a food composition in which the aforementioned baby watermelon extract salt is added by replacing the sodium component in the food composition.
본 발명에 따라 제조된 애기수박 추출 조성물은 부종완화의 효과를 나타냄으로써, 다양한 식품 분야에 활용될 경우 연간 폐기되는 애기수박의 활용가치를 극대화함으로써, 궁극적으로는 수박 농가의 고질적인 애기수박 처리 문제를 해결할 수 있을 것으로 기대하고 있다. The baby watermelon extract composition prepared according to the present invention shows the effect of edema relief, thereby maximizing the utilization value of baby watermelon that is discarded annually when used in various food fields, and ultimately, the chronic problem of watermelon watermelon treatment of watermelon farms. hope to be able to solve it.
상기 애기수박 추출 조성물은 크게 애기수박 추출 분말 및 애기수박 추출물 소금으로 분류될 수 있다. 상기 애기수박 추출 소금의 경우 기족 식품에 첨가되는 소금 및 나트륨을 대체함과 동시에 식품에 부종완화의 기능성을 제공함으로써, 식품의 부가 가치를 향상시킬 수 있다. 또한, 본 발명에 따른 애기수박 추출물 소금은 기존 소금에 비하여 상대적으로 저염인 특성을 가지므로, 상기 애기수박 추출물 소금을 함유한 식품 섭취를 통하여 건강증진을 도모할 수 있다. The baby watermelon extract composition can be largely classified into baby watermelon extract powder and baby watermelon extract salt. In the case of the baby watermelon extract salt, it is possible to improve the added value of food by replacing salt and sodium added to food and at the same time providing the functionality of edema relief to food. In addition, since the baby watermelon extract salt according to the present invention has a relatively low-salt characteristic compared to conventional salt, health promotion can be promoted through intake of food containing the baby watermelon extract salt.
한편, 상기 애기수박 추출 조성물은 추출 과정에서 미네랄이 함유된 증류수를 추출 용매로 사용하고, 구체적으로는 열처리된 현무암 유래 분말로 전처리를 거치기 때문에 추출 효율이 우수할 뿐만 아니라 소금을 이루는 성분 외에 다양한 무기 염류를 제공할 수 있다. 나아가 본 발명의 추출 용매는 건조된 애기수박의 추출 효율 증가 뿐만 아니라 증류수와 비교하여 애기수박으로부터 추가적인 성분의 추출을 유도하는 것으로 판단되고 있다. On the other hand, the baby watermelon extract composition uses distilled water containing minerals as an extraction solvent in the extraction process, and specifically, because it undergoes pretreatment with heat-treated basalt-derived powder, it not only has excellent extraction efficiency, but also various inorganic substances other than components constituting salt. Saline may be provided. Furthermore, it is believed that the extraction solvent of the present invention not only increases the extraction efficiency of dried baby watermelon, but also induces extraction of additional components from baby watermelon compared to distilled water.
도 1은 본 발명의 일 실시예에 따른 부종완화용 애기수박 추출 조성물의 제조방법을 개념적으로 설명하기 위한 순서도이다.
도 2는 도 1의 절편 건조단계 이후의 건조된 절편을 보여주는 사진이다.
도 3은 실험에 사용된 애기수박 추출 분말 및 애기수박 추출물 소금을 보여주는 사진이다.
도 4는 실험 전후의 대조군 및 실험군 쥐들의 부종 상태를 비교하기 위한 사진이다.
도 5는 실험결과 각 대조군 및 실험군에서의 부종 발생율을 도시한 그래프이다.
도 6은 실험결과 각 대조군 및 실험군에서의 부종 억제율을 도시한 그래프이다. 1 is a flowchart for conceptually explaining a method for preparing a baby watermelon extract composition for edema relief according to an embodiment of the present invention.
Figure 2 is a photograph showing the dried slices after the slice drying step of Figure 1.
3 is a photograph showing the baby watermelon extract powder and baby watermelon extract salt used in the experiment.
Figure 4 is a photograph for comparing the edema state of control and experimental rats before and after the experiment.
5 is a graph showing the incidence of edema in each control group and experimental group as a result of the experiment.
6 is a graph showing the edema inhibition rate in each control group and experimental group as a result of the experiment.
이하, 첨부된 도면, 사진 및 그래프 등을 참조하여 본 발명의 일 실시예에 따른 부종완화용 애기수박 추출 조성물의 제조방법 및 상기 애기수박 추출 조성물의 부종완화 효과에 대하여 자세하게 설명하도록 한다. 그러나 하기 설명들은 본 발명을 구체화하여 설명하기 위한 예시적인 기재들이며 하기 설명들에 의항 본 발명의 기술사상이 제한 해석되지 않는다. 본 발명의 기술사상은 오직 후술하는 청구범위에 의하여 해석되고 제한 될 수 있다. Hereinafter, a method for preparing a baby watermelon extract composition for edema relief according to an embodiment of the present invention and an edema relieving effect of the baby watermelon extract composition will be described in detail with reference to the accompanying drawings, photographs and graphs. However, the following descriptions are exemplary descriptions for specifically describing the present invention, and the technical spirit of the present invention is not limited and interpreted according to the following descriptions. The technical spirit of the present invention can be interpreted and limited only by the claims described below.
도 1은 본 발명의 일 실시예에 따른 부종완화용 애기수박 추출 조성물의 제조방법을 개념적으로 설명하기 위한 순서도이다. 1 is a flow chart for conceptually explaining a method for preparing a baby watermelon extract composition for edema relief according to an embodiment of the present invention.
도 1을 참조하면, 부종완화용 애기수박 추출 조성물의 제조(S1000)를 위해서는 우선, 건조된 애기수박 절편을 준비하는 단계 및 추출용매 준비단계(S210)를 수행한다. Referring to FIG. 1 , in order to prepare a baby watermelon extract composition for edema relief (S1000), first, a step of preparing dried baby watermelon slices and an extraction solvent preparation step (S210) are performed.
건조된 애기수박 절편을 준비하기 위해서는, 애기수박 수거단계(S110) 및 절편 건조단계(S120)를 거쳐야 한다. 애기수박 수거단계(S110)는 농가로부터 노지로 부터 수거되어 폐기되지 않은 애기수박을 제공받음으로써 이루어질 수 있으며, 향후 애기수박을 이용한 다양한 식품 산업이 발전할 경우에는 별도로 애기수박을 보존하기 위한 저장 수단이 강구되어야 할 것이다. In order to prepare dried baby watermelon slices, it is necessary to go through a harvesting baby watermelon step (S110) and a drying step (S120). The baby watermelon collection step (S110) can be performed by receiving the baby watermelon collected from the farmhouse and not discarded, and in the future, when a variety of food industries using the baby watermelon develop, a separate storage means for preserving the baby watermelon. this will have to be pursued
상기 수거된 애기수박은 소정의 크기로 절단되어 절편화된 후 건조되는 절편 건조단계(S120)를 거치는 데, 상기 절편의 크기는 추출 시설의 규모 등을 고려하여 적절히 고려될 수 있다. 한편, 건조 방식은 특별히 제한되지 않으며 노지에서 건조되거나 별도로 수집되어 단시간에 열풍 등에 의하여 건조될 수도 있다. The collected baby watermelon is cut to a predetermined size, sliced, and then goes through a slice drying step (S120) in which it is dried. The size of the slices may be appropriately considered considering the size of the extraction facility. On the other hand, the drying method is not particularly limited and may be dried in the open field or separately collected and dried by hot air in a short time.
도 2는 도 1의 절편 건조단계 이후의 건조된 절편을 보여주는 사진이다.Figure 2 is a photograph showing the dried slices after the slice drying step of Figure 1.
도 2를 참조하면, 건조된 절편은 도 2에서 보는 바와 같이 복수의 바람 구멍을 갖는 지지대 위에서 자연 건조되어 준비될 수 있다. 본 실시예에서, 상기 애기수박은 껍질 및 과육을 모두 포함한 상태로 절편화되어 건조된다. Referring to FIG. 2 , the dried slices may be prepared by being naturally dried on a support having a plurality of wind holes as shown in FIG. 2 . In this embodiment, the baby watermelon is sliced and dried in a state including both skin and pulp.
건조된 절편이 준비되면, 열수추출 단계(S200)이 진행되는데, 그 전에 미리 전술한 바와 같이 추출용매 준비단계(S210)가 이루어진다. 추출용매 준비단계는 시중에서 입수한 현무암을 파쇄하고 이를 800 내지 1000℃에서 소성(열처리)한 수 냉각 및 분말화 과정을 거쳐 준비될 수 있다. 이와 같이 현무암 열처리 분말(현무암 유래 미네랄)은 원래의 현무암과는 결정구조의 변화에 따라 물성이 변화된다. 준비된 현무암 열처리 분말을 증류수에 첨가하고, 1 내지 2주간 방치함으로써, 현무암 열처리 분말이 물과 충분히 작용할 수 있도록 한다. 방치가 완료되면, 현무암 분말은 여과되고, 여과된 후의 증류수만을 분리하여 추출용매로 사용한다. When the dried slices are prepared, the hot water extraction step (S200) proceeds, and before that, the extraction solvent preparation step (S210) is performed as described above. The extraction solvent preparation step may be prepared by crushing commercially available basalt and calcining (heat treatment) it at 800 to 1000 ° C, water cooling, and powdering. In this way, the heat-treated basalt powder (basalt-derived mineral) has different physical properties from the original basalt according to the change in crystal structure. The prepared basalt heat treatment powder is added to distilled water and left for 1 to 2 weeks, so that the basalt heat treatment powder can sufficiently interact with water. When the standing is complete, the basalt powder is filtered, and only the filtered distilled water is separated and used as an extraction solvent.
열수추출 단계(S200)에서 준비된 추출용매에 건조된 애기수박 절편을 첨가하고 대략 2 내지 10 시간 동안 추출이 이루어진다. 추출 시간과 추출 온도 등은 특별히 제한되지 않는다. Dried baby watermelon slices are added to the extraction solvent prepared in the hot water extraction step (S200), and extraction is performed for about 2 to 10 hours. The extraction time and extraction temperature are not particularly limited.
추출액이 준비되면, 농축 단계(S400)를 수행한다. 농축단계는 상기 추출액의 농도를 증가시키 위한 과정이며, 반복적인 가열 등을 통하여 이루어질 수 있으며, 농축의 방식은 크게 제한되지 않는다. When the extract is prepared, a concentration step (S400) is performed. The concentration step is a process for increasing the concentration of the extract, and may be achieved through repetitive heating, etc., and the method of concentration is not significantly limited.
한편, 상기 추출액의 농축과 별도로, 애기수박 추출물 소금을 준비하기 위하여 일부의 추출액은 증발 단계(310)를 거친다. 증발 단계(S310)는 반복적으로 이루어질 수 있으며, 이 때, 추출액도 반복하여 추가될 수 있다. 이후, 증발 단계(S310)를 거친 후 잔류하는 고형분을 수거하는 고형분 수거단계(S320)를 거침으로써 애기수박 추출물 소금이 준비될 수 있다. On the other hand, apart from the concentration of the extract, a part of the extract goes through an evaporation step (310) to prepare the baby watermelon extract salt. The evaporation step (S310) may be made repeatedly, and at this time, the extract may also be repeatedly added. Thereafter, the baby watermelon extract salt may be prepared by going through the solids collection step (S320) of collecting the remaining solids after the evaporation step (S310).
한편, 열수추출 단계(S200)를 거쳐 농축된 농축액은 통상적인 동결건조 단계(S500)를 거침으로써 분말화될 수 있다. On the other hand, the concentrate concentrated through the hot water extraction step (S200) can be powdered by passing through a conventional freeze-drying step (S500).
따라서 상기 방법(S1000)을 통하여, 애기수박 추출 분말 및 애기수박 추출물 소금이 각각 준비될 수 있다. Therefore, through the above method (S1000), baby watermelon extract powder and baby watermelon extract salt may be prepared, respectively.
애기수박 추출 분말 또는 애기수박 추출물 소금은 각각 기능성 첨가제로서, 다양한 식품학적 허용 가능한 성분들과 혼합되어 부종완화 특성을 식품 조성물로서 제조될 수 있다. The Arabidopsis watermelon extract powder or the Arabidopsis watermelon extract salt, each as a functional additive, can be mixed with various food-acceptable ingredients to produce a food composition with edema relieving properties.
특히, 상기 애기수박 추출물 소금은, 기존 식품의 나트륨이나 소금 성분을 대체한 성분으로 포함됨으로써, 기존 식품의 맛을 유지하면서도 식품에 부종완화의 기능성을 부여할 수 있다. 나아가, 상기 애기수박 추출물 소금은 그 자체로 유통되어 기능성 소금으로써 제품와 될 수 있다. In particular, the baby watermelon extract salt is included as a component that replaces the sodium or salt component of existing foods, thereby imparting the functionality of edema relief to foods while maintaining the taste of existing foods. Furthermore, the baby watermelon extract salt can be distributed by itself and become a product as a functional salt.
이하, 구체적인 실험을 통하여, 전술한 애기수박 추출 분말 및 애기수박 추출물 소금의 부종완화 효과에 대하여 자세하게 설명하도록 한다. Hereinafter, the edema relieving effect of the above-mentioned baby watermelon extract powder and baby watermelon extract salt will be described in detail through specific experiments.
카라기닌 유발 쥐에서의 부종억제 실험Edema inhibition test in carrageenan-induced rats
1. 시료 준비1. Sample preparation
전술한 바와 같은 방법으로 준비된 애기수박 추출 분말(WM) 및 애기수박 추출물 소금(WMCS)을 준비하였다. 도 3은 실험에 사용된 애기수박 추출 분말 및 애기수박 추출물 소금을 보여주는 사진이다.Arabidopsis watermelon extract powder (WM) and Arabidopsis watermelon extract salt (WMCS) prepared by the method described above were prepared. 3 is a photograph showing the baby watermelon extract powder and baby watermelon extract salt used in the experiment.
도 3에서 보는 바와 같이 애기수박 추출 분말에 비하여 애기수박 추출물 소금의 색상이 다소 옅은 것을 확인할 수 있었다. As shown in FIG. 3, it was confirmed that the color of the baby watermelon extract salt was slightly lighter than that of the baby watermelon extract powder.
2. 실험 동물2. Experimental animals
본 실험에서 사용한 실험동물은 6주령 수컷 Blab/c 쥐(mice)을 샘타코 (Osan, Korea)에서 구입하여 일주일 간 순화기간을 거친 다음 사용하였다. 쥐의 평균 무게는 20 ~ 25g 으로 시료는 실험 실시 단회 경구 투여하였다. 실험동물 사육실은 12시간 간격으로 명암을 조절하였고, 온도는 23 ± 2℃, 습도는 50 ~ 60%를 유지하였다. Experimental animals used in this experiment were 6-week-old male Blab/c mice (mice) purchased from Samtaco (Osan, Korea) and used after a week of acclimatization. The average weight of the rat was 20 ~ 25g, and the sample was orally administered once during the experiment. In the experimental animal breeding room, the light and shade were adjusted at 12-hour intervals, and the temperature was maintained at 23 ± 2 °C and the humidity was 50 to 60%.
3. 실험군 설계3. Experimental group design
실험동물은 3개 군으로 분류되었으며 하기 표 1에서 보는 바와 같이, 증류수를 투여하고 1% 카라기닌(carrageenan)를 처리한 군(DW), 애기수박 추출 분말 1 g/kg을 투여하고 1% 카라기닌을 처리한 군(WM), 애기수박 추출물 소금 1 g/kg 투여하고 1% 카라기닌을 처리한 군(WMCS)으로 분류하였다. 군 구성 마리수의 산정은 3R의 원칙에 준하여 통계적 의미를 가리는 최소수를 사용 하였다.Experimental animals were classified into 3 groups, and as shown in Table 1 below, distilled water was administered and 1% carrageenan was treated (DW), Arabidopsis watermelon extract powder 1 g/kg was administered and 1% carrageenan was administered. They were classified into a group treated with guinin (WM) and a group treated with 1% carrageenan after administering 1 g/kg of baby watermelon extract salt (WMCS). The calculation of the number of group members was based on the 3R principle, and the minimum number covering statistical significance was used.
4. 부종의 유발4. Induces edema
마우스 발바닥의 용적을 체적변동 유량계(plethysmometer)로 측정한 후 각 실험군 별로 증류수, 애기수박 추출 분말, 애기수박 추출물 소금 1 g/kg 투여군 (WMCS) 1g/kg 체중으로 경구 투여하였다. 경구투여 30분 후에 마이스 뒷발바닥에 염증 유발 물질인 1％ 카라기닌 생리 식염수 0.1ml씩 피하 주사하여 부종을 유발시켰다. 부종 유발 후, 1시간 간격으로 4회에 걸쳐 부종의 용적을 측정한 후 실험물질 투여 전 용적을 표준으로 하여 부종율 및 부종 억제율을 각각 계산하였으며, 계산은 다음 수학식 1 및 2의 계산식에 의하였다. After measuring the volume of the soles of the mice with a plethysmometer, distilled water, baby watermelon extract powder, and baby watermelon extract salt 1 g/kg administration group (WMCS) were orally administered with 1 g/kg body weight for each experimental group. 30 minutes after oral administration, 0.1 ml of 1% carrageenan physiological saline, an inflammation-inducing substance, was subcutaneously injected into the soles of the hind feet of Mice to induce edema. After induction of edema, the volume of edema was measured four times at 1-hour intervals, and the edema rate and edema inhibition rate were calculated respectively using the volume before administration of the test substance as a standard. did
5. 통계처리5. Statistical processing
실험에 얻어진 결과는 평균±표준편차 (mean ± SD)로 나타내었다. 각 시험군의 유의성에 대한 통계는 대조군과 실험군 간의 통계학적 비교를 위해 One-way Anova SPSS v.12를 사용하여 분석하였으며, Duncan's 사후검정을 실시하여 (p < 0.05)이하일 때 통계적으로 유의한 것으로 판단하였다.The results obtained in the experiment were expressed as mean ± standard deviation (mean ± SD). Statistics on the significance of each test group were analyzed using One-way Anova SPSS v.12 for statistical comparison between the control group and the experimental group, and Duncan's post-hoc test was performed to determine that it was statistically significant when ( p < 0.05) or less. judged.
발 부종 측정 결과Foot edema measurement results
각각의 시료를 투여 전과 투여 60분 후에 발 부종율을 측정한 결과는 하기 표 2와 같다. 대조군(CON)에 비해 애기수박 추출 분말(WM)과 애기수박 추출물 소금(WMCS)의 부종율이 낮았으며, 부종억제율 또한 대조군에 비해 유의적으로 낮게 나타내었다. 이하, 첨부된 사진 및 도면을 참조하여 설명한다. The results of measuring the swelling rate of the feet before and 60 minutes after administration of each sample are shown in Table 2 below. Compared to the control group (CON), the edema rate of the baby watermelon extract powder (WM) and the baby watermelon extract salt (WMCS) was lower, and the edema inhibition rate was also significantly lower than that of the control group. Hereinafter, it will be described with reference to the attached photos and drawings.
도 4는 실험 전후의 대조군 및 실험군 쥐들의 부종 상태를 비교하기 위한 사진이다. 도 4에서 보는 바와 같이 상대적으로 애기수박 추출 분말(WM) 및 애기수박 추출물 소금(WMCS) 실험군에서 붓기가 낮게 관찰 되었다. Figure 4 is a photograph for comparing the edema state of control and experimental rats before and after the experiment. As shown in FIG. 4, relatively low swelling was observed in the experimental groups of baby watermelon extract powder (WM) and baby watermelon extract salt (WMCS).
도 5는 실험결과 각 대조군 및 실험군에서의 부종 발생율을 도시한 그래프이다. 5 is a graph showing the incidence of edema in each control group and experimental group as a result of the experiment.
도 5를 참조하면, 시료를 투여 전과 투여 60분 후에 족척 부종 증가를 측정한 결과 대조군은 83.2 ± 0.8% 증가한 반면 애기 수박 추출 분말(WM)과 애기수박 추출물 소금(WMCS) 실험군은 각각 37.2 ± 1.7% 및 35.3 ± 5.0%로서, 유의적으로 낮은 증가를 나타내었다.Referring to FIG. 5, as a result of measuring the increase in foot edema before and 60 minutes after the administration of the sample, the control group increased by 83.2 ± 0.8%, while the baby watermelon extract powder (WM) and the baby watermelon extract salt (WMCS) experimental group showed an increase of 37.2 ± 1.7, respectively. % and 35.3 ± 5.0%, showing a significantly lower increase.
도 6은 실험결과 각 대조군 및 실험군에서의 부종 억제율을 도시한 그래프이다. 6 is a graph showing the edema inhibition rate in each control group and experimental group as a result of the experiment.
도 6을 참조하면, 시료를 투여 전과 투여 60분 후에 족척 부종 증가억제율을 측정한 결과 대조군의 경우 시료 투여 전후에 변화가 없었으며, 애기수박 추출 분말WKM)과 애기수박 추출물 소금(WMCS) 실험군은 각각 55.3 ± 2.1% 및 57.5 ± 6.0%로서, 유의적으로 높은 억제율을 나타내었다.Referring to FIG. 6, as a result of measuring the increase in footpad edema before and after 60 minutes of administration of the sample, there was no change in the control group before and after the sample administration, and the Arabidopsis watermelon extract powder WKM) and the Arabidopsis watermelon extract salt (WMCS) experimental group 55.3 ± 2.1% and 57.5 ± 6.0%, respectively, showed significantly high inhibition rates.
Claims (7)
상기 애기수박을 절편화하여 건조하는 단계;
상기 건조된 애기수박 절편을 열수 추출하여 추출액을 준비하는 단계;
상기 추출된 추출액을 농축 및 동결건조하여 애기수박 추출 분말을 수득하는 단계를 포함하여 이루어지고,
상기 추출액을 반복적으로 증발시켜 추출액내 고형분인 애기수박 추출물 소금을 준비하는 단계를 더 포함하며,
상기 열수 추출은 미네랄 함유 증류수를 추출용매로 하여 이루어지며,
상기 미네랄 함유 증류수는, 증류수에, 현무암을 800 내지 1000℃에서 소성한 후 냉각하여 분말화된 현무암 유래 미네랄을 첨가하고 1 내지 2주간 방치한 후, 분말 성분을 여과한 후 수득되며,
상기 추출액을 준비하는 단계는, 상기 추출용매에 건조된 애기수박 절편을 첨가하고 2 내지 10시간 동안 추출과정을 수행하고,
상기 애기수박 추출물 소금을 준비하는 단계는, 상기 추출액의 일부를 증발시키는 단계와, 상기 증발시키는 단계를 거친 후에 상기 증발로 인해 잔류하는 고형분을 수거하는 고형분 수거단계를 포함하여 이루어지는 것을 특징으로 하는 부종완화용 애기수박 추출 조성물의 제조방법.
Collecting baby watermelon;
Slicing and drying the baby watermelon;
Preparing an extract by hot water extraction of the dried baby watermelon slices;
Concentrating and freeze-drying the extracted extract to obtain an extract powder of baby watermelon,
Further comprising the step of repeatedly evaporating the extract to prepare baby watermelon extract salt, which is a solid in the extract,
The hot water extraction is performed using mineral-containing distilled water as an extraction solvent,
The mineral-containing distilled water is obtained by calcining basalt at 800 to 1000 ° C., adding powdered basalt-derived minerals to distilled water, leaving it for 1 to 2 weeks, and filtering the powder component,
In the step of preparing the extract, dried baby watermelon slices are added to the extraction solvent and an extraction process is performed for 2 to 10 hours,
The step of preparing the baby watermelon extract salt comprises the step of evaporating a part of the extract, and the solids collection step of collecting the solids remaining due to the evaporation after the evaporation. Manufacturing method of baby watermelon extract composition for relief.
식품학적으로 허용가능한 식품 첨가물을 포함하는,
부종완화용 식품 조성물.
A baby watermelon extract composition for edema relief prepared by the method of claim 1 and
Including food-acceptable food additives,
Food composition for edema relief.
상기 부종완화용 애기수박 추출 조성물은,
카라기닌에 의하여 부종이 유발된 쥐(mice)에 투여하고 60분 경과 후,
대조군의 83.2 ± 0.8% 부종 증가율 대비,
각각 37.2 ± 1.7% 및 35.3 ± 5.0%의 부종 증가율을 나타내는 것을 특징으로 하는 부종완화용 식품 조성물.
According to claim 5,
The baby watermelon extract composition for edema relief,
60 minutes after administration to mice induced by carrageenan edema,
Compared to the 83.2 ± 0.8% increase in edema in the control group,
A food composition for edema relief, characterized in that it exhibits an edema increase rate of 37.2 ± 1.7% and 35.3 ± 5.0%, respectively.
A food composition for edema relief in which the baby watermelon extract composition for edema relief prepared by the method of claim 1 is added in place of the sodium component in the food composition.
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