KR102547706B1 - Rapid bio diagnostic kit - Google Patents

Abstract

The present invention includes a first substrate 100 in which a first through hole 103 is formed in a portion of a body 105; a second substrate 200 coupled to the lower surface of the first substrate 100, a second through-hole 204 formed in a part of the body, and a circuit pattern formed thereon; A rapid bio-diagnosis kit including a third substrate 300 coupled to the lower surface of the second substrate 200 is provided.

Description

Rapid bio diagnostic kit {Rapid bio diagnostic kit}

The present invention relates to a biodiagnostic kit, and more particularly, to a rapid biodiagnostic kit capable of fundamentally preventing leakage of an electrolyte solution.

A nanopore is a small hole (eg, having a diameter of about 1 nm to about 1000 nm) that can detect the flow of electrons through the hole by changing the ionic current and/or the tunneling current.

Each nucleotide of a nucleic acid (e.g., adenine, cytosine, guanine, thymine, uracil in RNA) in DNA physically passes through the nanopore Measuring the change in the current flowing through the nanopore can be used to directly sequence the nucleic acid molecules passing through the nanopore, as the current density affects the current density across the nanopore in a specific way.

Nanopore technology is based on electrical sensing, which can detect nucleic acid molecules in much smaller concentrations and volumes than required for other conventional sequencing methods.

1 schematically shows a solid-state based two-dimensional nanopore device according to the prior art.

In use, the nanopore device is placed in an intermediate chamber separating the top and bottom chambers such that the top and bottom chambers are fluidically coupled by nanopore pillars. The top, middle, and bottom chambers contain an electrolyte solution containing charged particles (eg, DNA) to be detected. Different electrolyte solutions can be used for detection of different charged particles.

A sensor including such a nanopore device is placed in a diagnostic kit, and the sensor's electrical signal is read by a reader.

2 illustrates the structure of a conventional diagnostic kit. Referring to FIG. 2, the conventional diagnostic kit forms an accommodation space 43 by stacking a plate 40 and a wall 41 on the upper surface of the plate 40, and in the accommodation space 43, a sensor including a nanopore device ( 30) is seated, and an electrolyte solution containing particles to be detected is injected into the receiving space 43 and the receiving groove 44, and then parts 11 and 21 of the positive power supply 10 and the negative power supply 20 are When power is applied through the reader in a state where it is exposed to the electrolyte solution, the electrolyte solution drawn by the power source flows through the nanopore device. In addition, an adhesive tape 42 is attached to the lower portion of the negative electrode 20 to prevent leakage of the electrolyte solution.

However, in the diagnosis kit having such a structure, there is a possibility that the electrolyte may leak through the anode 10 or the cathode 20. As described above, when the electrolyte solution leaks, there is a problem in that the reader is contaminated by, for example, viruses contained in the electrolyte solution, making it impossible to reuse the reader.

Therefore, there is a need for a biodiagnostic kit that can be reused without contaminating the reader and can stably supply or transmit electrical signals.

Korean Patent Publication No. 10-2020-0086267 (published on July 16, 2020)

The present invention is intended to provide a rapid bio-diagnostic kit that can be reused without contaminating the reader and can stably supply or transmit electrical signals.

The present invention relates to a first substrate having a first through-hole formed in a portion of a body; a second substrate coupled to a lower surface of the first substrate, a second through-hole formed in a portion of the body, and a circuit pattern formed thereon; A rapid bio-diagnosis kit including a third substrate coupled to a lower surface of the second substrate is provided.

According to one embodiment, the sensing unit and the electrolyte solution holding the sample are accommodated through the first through-hole.

According to one embodiment, the sensing unit includes a nanopore device.

According to an embodiment, a groove is formed on one side wall of the first through hole, and a copper foil circuit is coated or deposited on the surface of the groove to act as an anode, and a part of the electrolyte solution accommodated in the first through hole is formed on the surface of the groove. exposed to the anode.

According to an embodiment, a first electrolyte inlet through which an electrolyte solution can be injected and a first gas outlet through which gas can be discharged during electrolyte injection are formed outside the first through-hole of the first substrate.

According to an embodiment, a support part is formed around the second through-hole, the sensing part is seated on the support part, and the circuit formed in the sensing part is wire bonded to the circuit pattern of the second substrate formed around the support part. bonding).

According to one embodiment, a copper foil circuit for connection with the anode is formed on the upper surface of the second substrate.

According to one embodiment, a cathode copper foil circuit operating as a cathode is formed on the lower surface of the second substrate.

According to one embodiment, a second electrolyte inlet and a second gas outlet are formed through the second substrate.

According to one embodiment, the second electrolyte inlet and the second gas outlet communicate with the first electrolyte inlet and the first gas outlet formed on the first substrate, respectively.

According to one embodiment, an electrolyte injection groove and a gas discharge groove are formed on a part of the body of the third substrate.

According to one embodiment, an electrolyte receiving groove capable of accommodating an electrolyte solution is formed inside the third substrate.

According to one embodiment, the electrolyte receiving groove communicates with the electrolyte injection groove and the gas discharge groove.

According to an embodiment, when the electrolyte is injected through the electrolyte injection groove, the gas present in the electrolyte receiving groove is discharged to the outside along the gas discharge groove, and the electrolyte solution is accommodated in the electrolyte receiving groove.

According to one embodiment, the second substrate is formed longer in the longitudinal direction than the first substrate and the third substrate, and a connection portion capable of being combined with a connector of a reader is formed on one side of the protruding second substrate. do.

According to one embodiment, the first substrate, the second substrate and the third substrate are formed of a PCB material.

According to one embodiment, the PCB material may be any one of paper phenol, paper polyester, paper epoxy, glass paper epoxy, glass based epoxy, or glass cloth epoxy.

According to one embodiment, the first substrate, the second substrate and the third substrate are firmly adhesively bonded by a multi-layer PCB stacking method so that the electrolyte solution does not leak.

According to an embodiment, the first substrate and the third substrate may be thicker than the second substrate 200 .

According to an embodiment, a negative electrode copper foil circuit operating as a negative electrode may be formed on a lower surface of the second substrate, and an electrolyte solution accommodated in the electrolyte receiving groove may be exposed to the negative electrode copper foil circuit.

The present invention has an effect of fundamentally blocking the leakage of the electrolyte solution through the positive electrode and the negative electrode in contact with the electrolyte solution.

The present invention has the effect of stably injecting the electrolyte into the electrolyte receiving groove.

The present invention uses a plurality of PCB substrates as raw materials and manufactures them by a multilayer stacking method, so that leakage of the electrolyte solution through the bonding surface does not occur.

1 is a schematic diagram of a two-dimensional nanopore device according to the prior art.
2 is a schematic diagram of a conventional diagnostic kit.
3 is a perspective view of a bio-diagnosis kit according to an embodiment of the present invention;
4 is a conceptual view of front and rear surfaces of a second substrate according to an embodiment of the present invention;
5 is a perspective view of a third substrate according to an embodiment of the present invention;
Fig. 6 A cutaway view along line AA of Fig. 3;
7 is a real picture of a bio-diagnosis kit before mounting a sensing unit according to an embodiment of the present invention.
8 is a real picture of a bio-diagnosis kit after mounting a sensing unit according to an embodiment of the present invention.

Embodiments of the present disclosure are illustrated for the purpose of explaining the technical idea of the present disclosure. The scope of rights according to the present disclosure is not limited to the specific description of the embodiments or these embodiments presented below.

All technical terms and scientific terms used in this disclosure have meanings commonly understood by those of ordinary skill in the art to which this disclosure belongs, unless defined otherwise. All terms used in this disclosure are selected for the purpose of more clearly describing the disclosure and are not selected to limit the scope of rights according to the disclosure.

Expressions such as "comprising", "including", "having", etc. used in this disclosure are open-ended terms that imply the possibility of including other embodiments, unless otherwise stated in the phrase or sentence in which the expression is included. (open-ended terms).

Expressions in the singular form described in this disclosure may include plural meanings unless otherwise stated, and this applies equally to expressions in the singular form described in the claims.

Referring to FIG. 3 , the rapid bio-diagnosis kit of the present invention is coupled to a first substrate 100 having a first through-hole 103 formed in a part of a body 105 and a lower surface of the first substrate 100, and It includes a second substrate 200 on which a second through hole 204 is formed and a circuit pattern formed thereon, and a third substrate 300 coupled to a lower surface of the second substrate 200 .

The first substrate 100 has a rectangular plate shape with a thin thickness. The first through-hole 103 formed in the body 105 of the first substrate 100 may accommodate a sensing unit 400 to be described later and an electrolyte solution holding a sample.

Here, the sample refers to a solution to be detected, and is a substance suspected of containing an analyte. For example, the sample may include saliva, mucus, blood, cerebrospinal fluid, sweat, urine, ascites, and the like.

In addition, the specimen may be used directly obtained from a biological source, or may be used after being subjected to a preliminary treatment to modify the characteristics of the specimen. Pretreatment may include methods such as filtration, precipitation, dilution, mixing, concentration, inactivation of interfering components, lysis, reagent addition, and the like.

The first through hole 103 can be obtained by punching a part of the body of the first substrate 100 . The shape of the first through hole 103 is not limited, but preferably, the first through hole 103 may have a rectangular cross section.

The sensing unit 400 may be a biosensor including a nanopore device. The biosensor may receive power through a circuit pattern formed on the first substrate 100 or the second substrate 200 or may transmit a signal to a reader (not shown).

A groove 104 may be formed on one side wall of the first through hole 103 . Preferably, the groove 104 may have a semicircular cross section extending from the upper surface to the lower surface of the body 105 . A copper foil circuit may be coated or deposited on the surface of the groove 104 to act as an anode. Accordingly, when the electrolyte solution is injected into the first through hole 103, a portion of the electrolyte solution may be exposed to the anode.

Outside the first through-hole 103 of the first substrate 100, there is a first electrolyte inlet 101 through which an electrolyte solution can be injected and a gas (in particular, air) present inside the substrate can be discharged during electrolyte injection. The first gas outlet 102 may be formed through.

Preferably, the shapes of the first electrolyte inlet 101 and the first gas outlet 102 are not limited as long as they can pass through the first substrate 100, and may preferably have a circular cross-section.

Referring to FIG. 4 , the upper surface of the second substrate 200 may be adhesively bonded to the lower surface of the first substrate 100 . The second substrate 200 may have a rectangular plate shape having a thin thickness. Circuits or circuit patterns for power supply from the reader and data communication with the reader may be formed on the upper and lower surfaces of the second substrate 200 .

A connection portion 207 capable of receiving power from the reader or exchanging data with the reader may be formed on one side of the second substrate 200 . For example, the connection unit 207 may be implemented in a form similar to USB (Universal Serial Bus).

The second substrate 200 may be formed longer in the longitudinal direction than the first substrate 100 and the third substrate 300 . Therefore, since the connection part 207 of the second board 200 can protrude from the first board 100 and the third board 300, it can be easily combined with the connector of the reader.

A second through hole 204 may be formed in the body of the second substrate 200 . The second through hole 204 can be obtained by drilling a part of the second substrate 200 . Preferably, the second through hole 204 may have a rectangular cross section.

The area of the second through hole 204 may be smaller than that of the first through hole 103 formed in the first substrate 100 .

A support portion 205 on which no circuit pattern is formed may be formed around the second through hole 204 . The sensing unit 400 may be seated on the support unit 205 . The circuit formed on the sensing unit 400 may be connected to the circuit of the second substrate 200 formed around the support unit 205 by wire bonding.

When the wire bonding portion is molded with epoxy, the sensing unit 400 can be firmly supported on the second substrate 200 and the wire bonding portion can be protected.

A copper foil circuit 203 for connection with an anode formed on the surface of the groove 104 of the first substrate 100 may be formed outside the support part 205 .

Furthermore, a cathode copper foil circuit 206 operating as a cathode may be formed on the lower surface of the second substrate 200 corresponding to the support portion 205 . The cathode copper foil circuit 206 may be exposed to the electrolyte solution by a method described below.

A second electrolyte inlet 201 and a second gas outlet 202 may be formed through the second substrate 200 . The shapes of the second electrolyte inlet 201 and the second gas outlet 202 are not limited as long as they can pass through the second substrate 200 .

The second electrolyte inlet 201 and the second gas outlet 202 may communicate with the first electrolyte inlet 101 and the first gas outlet 102 formed on the first substrate 100 , respectively.

Referring to FIG. 5 , an electrolyte injection groove 301 and a gas discharge groove 302 may be formed in a part of the body 304 of the third substrate 300 . An electrolyte receiving groove 303 capable of accommodating an electrolyte solution therein is formed in the third substrate 300, and the electrolyte receiving groove 303 can communicate with the electrolyte injection groove 301 and the gas discharge groove 302. there is.

The electrolyte injection groove 301 and the gas discharge groove 302 may have a ring shape, and the electrolyte receiving groove 303 may have a rectangular shape, but it is not limited as long as the electrolyte flows or can accommodate it.

When the electrolyte is injected through the electrolyte injection groove 301, the gas existing inside the electrolyte receiving groove 303 is discharged to the outside along the gas discharge groove 302, and the electrolyte solution can be accommodated in the electrolyte receiving groove 303. there is.

If the area of the electrolyte accommodating groove 303 is larger than the area of the cathode copper foil circuit 206 formed under the second substrate 200, the electrolyte solution accommodated in the electrolyte accommodating groove 303 will be exposed to the cathode copper foil circuit 206. can

Referring to FIG. 6 , the first substrate 100 , the second substrate 200 and the third substrate 300 may be adhesively bonded to each other. More specifically, the lower surface of the first substrate 100 and the upper surface of the second substrate 200 are adhesively bonded, and the lower surface of the second substrate 200 and the upper surface of the third substrate 200 are adhesively bonded. can do.

The first substrate 100, the second substrate 200, and the third substrate 300 have the same width, but the second substrate 200 is longer than the first substrate 100 and the third substrate 300. It can be formed longer in the direction.

As such, since the second substrate 200 is longer in the longitudinal direction than the first substrate 100 and the third substrate 300, a connection portion 207 may be formed on one side of the protruding second substrate 200, , the connector 207 can easily engage with the connector of the reader.

In addition, the first through-hole 103 of the first substrate 100 and the second through-hole 204 of the second substrate 200 may be coupled to exist at corresponding positions. Accordingly, the sensing unit 400 may be seated on the support part 205 formed around the second through hole 204 of the second substrate 200 through the first through hole 103 of the first substrate 100. .

The first electrolyte inlet 101 and the first gas outlet 102 of the first substrate 100 communicate with the second electrolyte inlet 201 and the second gas outlet 201 formed on the second substrate 300, respectively. can be combined Furthermore, the second electrolyte inlet 201 and the second gas outlet 202 of the second substrate 200 are coupled to communicate with the electrolyte inlet 301 and the gas outlet 302 of the third substrate 300. can

Therefore, when the electrolyte solution is injected through the first electrolyte injection hole 101, the injection flow of the electrolyte solution is formed along the first electrolyte injection hole 101, the second electrolyte injection hole 201, and the electrolyte injection groove 301, An electrolyte solution may be accommodated in the electrolyte receiving groove 303, and a gas discharge passage is formed along the first gas discharge port 102, the second gas discharge port 201, and the gas discharge groove 302 to discharge the gas to the outside. It can be.

The first substrate 100, the second substrate 200 or the third substrate 100 may be formed of a general PCB material. That is, the first substrate 100, the second substrate 200, or the third substrate 100 is made of any one of paper phenol, paper polyester, paper epoxy, glass paper epoxy, glass base epoxy, or glass cloth epoxy. can be formed

Circuit patterns are not printed on the first substrate 100 and the third substrate 300, but may be formed of a PCB material to improve assembly and adhesion with the second substrate 200.

As described above, the first substrate 100 and the third substrate 300 use a PCB material that is not coated with copper foil and the second substrate 200 uses a PCB material on which a copper foil circuit is formed, so that the first, second, and third substrates are It can be firmly adhesively bonded by a known multi-layer PCB lamination method.

In the case of using a PCB material for the first substrate 100, the second substrate 200, and the third substrate 300 as in the present invention and combining the substrates in a multi-layer PCB stacking method, the electrolyte is applied to the first, second, and third substrates. It does not leak between the adhesive parts of (100, 200, 300). Therefore, even if the reader is connected to the connection part 207 of the second substrate 200, the reader is not contaminated and the reader can be reused.

The first substrate 100 and the third substrate 300 may be thicker than the second substrate 200 . Preferably, the first substrate 100 and the third substrate 300 may have a thickness of 1.6 mm, and the second substrate 200 may have a thickness of 1 mm.

Alternatively, the first substrate 100 and the third substrate 300 may have the same thickness or different thicknesses. That is, when the thickness of the sensing unit 400 is thick, the first substrate 100 may be thick enough to accommodate the sensing unit 400 . In addition, when a large amount of electrolyte solution is to be accommodated, the thickness of the third substrate 300 may be adjusted in proportion thereto.

As described above, the sensing unit 400 is fixed to the support part 205 of the second substrate 200 through the first through hole 103 of the first substrate 100, and the first through hole of the first substrate 100 (103) An anode in the shape of a groove 104 is formed on one side wall surface, a cathode is formed on the lower surface of the second substrate 200, and an electrolyte receiving groove 303 formed by the cathode on the third substrate 300 When it is formed to be located inside and power is applied to the anode and the cathode, the electrolyte solution can move between the first through hole 103, the sensing unit 400, and the electrolyte receiving groove 303.

7 shows a real picture of the bio-diagnosis kit according to the present invention before the sensor 400 is mounted.

8 shows a real picture of the bio-diagnosis kit according to the present invention after the sensor 400 is mounted.

Although the foregoing embodiments have been described with respect to the most preferred examples of the present invention, it is not limited to only the above embodiments, and it is clear to those skilled in the art that various modifications are possible without departing from the technical spirit of the present invention.

30: sensor
40: plate
41: wall
42: adhesive tape
43: accommodation space
10, 20: anode, cathode
100: first substrate
200: second substrate
300: third substrate
101: first electrolyte inlet
102: first gas outlet
103: first through hole
104: Home
201: second electrolyte inlet
202: second gas outlet
203: copper foil circuit
204: second through hole
205: support
206: cathode copper foil circuit
207: connection part
301: electrolyte injection groove
302: gas discharge groove
303: electrolyte receiving groove
400: sensing unit

Claims (20)

a first substrate having a first through-hole formed in a portion of the body;
a second substrate coupled to a lower surface of the first substrate, a second through-hole formed in a portion of the body, and a circuit pattern formed thereon;
And a third substrate coupled to the lower surface of the second substrate,
Wherein the first substrate, the second substrate and the third substrate are formed of a PCB material.
The rapid bio-diagnosis kit according to claim 1, wherein the sensing unit and the electrolyte solution holding the sample are accommodated through the first through-hole.
The rapid bio-diagnosis kit according to claim 2, wherein the sensing unit is a bio-sensor including a nanopore device.
The method of claim 2, wherein a groove is formed on one side wall of the first through hole, and a copper foil circuit is coated or deposited on the surface of the groove to act as an anode, and a portion of the electrolyte solution accommodated in the first through hole is formed on the surface of the groove. A rapid bio-diagnosis kit that is exposed to an anode.
The rapid bio-diagnosis kit according to claim 1, wherein a first electrolyte inlet through which an electrolyte solution can be injected and a first gas outlet through which gas can be discharged when the electrolyte is injected are formed outside the first through-hole of the first substrate.
4 . The method of claim 3 , wherein a support on which the sensing unit is seated is formed around the second through-hole, and a circuit formed in the sensing unit is wire bonded to the circuit pattern of the second substrate formed around the support. A rapid bio-diagnostic kit that is connected in the same way.
The rapid bio-diagnosis kit according to claim 4, wherein a copper foil circuit for connection to the anode is formed on the upper surface of the second substrate.
The rapid bio-diagnosis kit according to claim 7, wherein a cathode copper foil circuit operating as a cathode is formed on the lower surface of the second substrate.
The rapid bio-diagnosis kit according to claim 5, wherein a second electrolyte inlet and a second gas outlet are formed through the second substrate.
The rapid bio-diagnosis kit according to claim 9, wherein the second electrolyte inlet and the second gas outlet communicate with the first electrolyte inlet and the first gas outlet formed on the first substrate, respectively.
The rapid bio-diagnosis kit according to claim 1, wherein an electrolyte injection groove and a gas discharge groove are formed on a part of the body of the third substrate.
The rapid bio-diagnosis kit according to claim 11, wherein an electrolyte accommodating groove capable of accommodating an electrolyte solution is formed inside the third substrate.
The rapid bio-diagnosis kit according to claim 12, wherein the electrolyte receiving groove communicates with the electrolyte injection groove and the gas discharge groove.
14. The rapid bio-diagnosis kit according to claim 13, wherein when the electrolyte is injected through the electrolyte injection groove, the gas present in the electrolyte receiving groove is discharged to the outside along the gas discharge groove, and the electrolyte solution is accommodated in the electrolyte receiving groove. .
The method of claim 1, wherein the second substrate is longer than the first substrate and the third substrate in a longitudinal direction, and a protruding second substrate has a protruding portion formed at one side of the second substrate to be coupled with a connector of the reader. , rapid bio-diagnostic kit.
delete The rapid bio-diagnostic kit according to claim 1, wherein the PCB material is any one of paper phenol, paper polyester, paper epoxy, glass paper epoxy, glass based epoxy, and glass cloth epoxy.
The rapid bio-diagnosis kit according to claim 17, wherein the first substrate, the second substrate, and the third substrate are firmly adhesively bonded by a multi-layer PCB stacking method so that the electrolyte solution does not leak.
The rapid bio-diagnosis kit according to claim 1, wherein the first substrate and the third substrate are thicker than the second substrate.
13. The rapid bio-diagnosis kit according to claim 12, wherein a cathode copper foil circuit serving as a cathode is formed on the lower surface of the second substrate, so that the electrolyte solution accommodated in the electrolyte receiving groove can be exposed to the cathode copper foil circuit.

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