KR102543764B1 - Primo node monitoring technology in the skin - Google Patents

Abstract

The present invention relates to a method for observing skin primo nodes.

Description

How to Observe the Skin Primo Node {PRIMO NODE MONITORING TECHNOLOGY IN THE SKIN}

The present invention relates to a method for observing skin primo nodes.

Since the meridian theory of oriental medicine is still a theory and has no anatomical histological substance, treatments such as acupuncture or meridian massage using the meridian theory have been criticized in the medical world as unscientific treatment.

However, recently, the primo duct in the intra-abdominal lymphatic vessel was successfully stained through the caudate vein infusion staining method using Alcian blue, but there has been no case of staining and observing skin nodes to date.

The meridian is a passageway for the circulation of energy and blood, connecting the inside and outside of the human body, and is linked to the five organs, limbs, five organs, skin, muscles, veins, and bones, enabling organic human body activities. All tissues and organs inside and outside the body can maintain normal physiological activities only when they are supplied with energy and blood through the meridians. Acupuncture, one of the representative treatments of oriental medicine, is a treatment that causes a reaction in the living body by giving physical stimulation to a certain area on the body surface, and the stimulation area is mainly the acupoints and meridians.

The theory of Bonghan is a hypothesis about the meridian theory advocated by Dr. This study was reviewed in the early 2000s by Professor So Kwang-seop's research team at Seoul National University, and it was called the PrimoVascular Circulatory System (PVS).

The present invention provides a method for staining skin primo nodes in the dermal and epidermal layers of the skin using Evans Blue solution.

In order to solve the problem, the present invention provides a method for dyeing skin primo nodes.

The method of the present invention can clearly reveal the relationship between acupuncture points in oriental medicine and skin primonode. The skin primo node can be used as an acupuncture point in oriental medicine and can be used as a route for diagnosing diseases and administering treatments. It can also be used as a target for new physical therapy. It is the basis for revealing the scientific efficacy of modern massage therapy.

By using the method of the present invention, the state of the skin primo node can be analyzed by physical and biochemical methods and used for diagnosis of disease. In addition, it can be used as a basis for drug delivery for the purpose of treating diseases through the skin primo node. In addition, it can be a basis for applying acupuncture stimulation, light stimulation, and heat stimulation for disease treatment targeting skin primo nodes.

1 to 13 are photographs and graphs for explaining experimental examples of the present invention.

The terms or words used in this specification and claims should not be construed as being limited to their ordinary or dictionary meanings, and the inventors can properly define the concept of terms in order to best explain their invention. Based on the principle, it should be interpreted as a meaning and concept consistent with the technical idea of the present invention.

Therefore, the matters described in the embodiments, reference examples, and drawings described in this specification are only the most preferred examples of the present invention and do not represent all of the technical spirit of the present invention, so that they can be replaced at the time of the present application. It should be understood that there may be many equivalents and variations.

Several attempts have been made to scientifically prove the reality of the meridians and meridians, and thus, research has been conducted on the Primo circulatory system of the skin, which has not yet been clearly identified.

Evans Blue was injected intravenously into the Sprague Dawley Rat, and unlike normal skin tissue, blue-dyed nodes were found in the epidermal layer of the skin. By observing that they are connected to form a network, it was discovered that the skin nodes are primo nodes in the dermal and outer dermal layers of the skin.

In addition, as a result of examining differences in gene expression in the three groups by collecting lymph nodes, differences in gene expression levels were shown between skin and skin primo nodes, and in skin primo nodes, the number of genes involved in cell differentiation and nerve regeneration was relatively high. has been expressed a lot with This can be evaluated as an antenna that detects the safety and danger of the external environment by detecting harmful environments such as temperature, humidity, pressure, fine dust, etc. there is.

Example 1. Evans Blue skin primo node observation experiment

To date, dyes such as Alcian blue, Trypan blue, Toluidine blue, Phalloidin, Methylen blue, Janus Green B Methods such as , DAPI, etc. have been proposed, but observation on the skin surface has failed until recently due to toxicity or insignificant dyeing effect. Therefore, the observation of the skin node was confirmed through the following experimental method.

7-week-old RAT males were used as animals (see Fig. 1).

1. Anesthesia (Alphaxic acid 0.4cc + Rumpun 0.1cc)

2. Hair Removal

3. Dye injection (inject 5% Evans Blue 0.2cc into tail vein)

4. Observation of dyed Primo nodes on the skin surface after 30 minutes
; Direct observation without tools as shown in FIG. 3 or CT observation as shown in FIG. 5

5. Observation of stained organ surface primo node and lymphatic primo node after laparotomy

Figure 2 is a photograph of the Primo circulatory system found in the epidermis. Figure 3 is a node found in the skin. In FIG. 4, A is a skin primo node dyed with Evans Blue, and B is the opposite side of the dyed skin primo node. Nodes are connected to each other. 5 is a three-dimensional CT cross-sectional view of a skin primo node. Figure 6 : Table 1 diferentially expressed genes(DEG) of Group 1(skin primo nodes) vs Group2(skin) in rat (only Group 1 expressed gene) Figure 9. Indicates the percentage of significantly expressed genes between G1 and G2 by classifying gene function into 14 categories. Figure 11. Indicates the percentage of significantly expressed genes between G1 and G3 by classifying gene function into 14 categories. Figure 12. Classify the functions of predominantly expressed genes into 14 categories comparing G1 vs G2. Figure 13. Classify the functions of predominantly expressed genes into 14 categories comparing G1 vs G3.

The above description is merely an example of the technical idea of the present invention, and various modifications and variations can be made to those skilled in the art without departing from the essential characteristics of the present invention.

Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but to explain, and the scope of the technical idea of the present invention is not limited by these embodiments. The protection scope of the present invention should be construed according to the claims below, and all technical ideas within the equivalent range should be construed as being included in the scope of the present invention.

Claims (2)

As a method of visually observing skin primonodes distributed on the skin of animals other than humans,
depilating skin hair;
staining the skin primo node by injecting 5% concentration Evans Blue into the caudate vein; and
Observing skin primo nodes distributed in the epidermis and dermis by directly observing the skin surface without tools to confirm that they are connected to each other by multi-tube tubular structures to form a network; including, visually inspecting skin primo nodes how to observe. delete

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