KR102541989B1 - The strain of Komagataeibacter intermedius NY-Gah 150-2 and method for producing industrial biogel with excellent productivity and tensile strength using the same - Google Patents

Abstract

The present invention relates to Komagataeibacter intermedius NY-Gah 150-2 strain or Gluconacetobacter europaeus NY-Gch 187-1 strain, and a biogel production method using the same. , Since biogel with excellent physical properties can be produced in high yield by using the above strain, it can be used as a pure biogel for medical, beauty, food, electrical and electronic fields or new functional materials for food, depending on the purpose, for industrial use and can be used for research.

Description

The strain of Komagataeibacter intermedius NY-Gah 150-2 strain and method for producing industrial biogel with excellent productivity and tensile strength using the strain {The strain of Komagataeibacter intermedius NY-Gah 150-2 and method for producing industrial biogel with excellent productivity and tensile strength using the same}

The present invention relates to Komagataeibacter intermedius NY-Gah 150-2 strain or Gluconacetobacter europaeus NY-Gch 187-1 strain, and a biogel production method using the same .

Cellulose is the most abundant biomaterial resource in nature, and in particular, cellulose produced by acetic acid bacteria is receiving a lot of attention as a new high-value industrial material as well as a food additive.

Since it was reported that acetic acid bacteria produce cellulose, cellulose produced by microorganisms (Bacterial Cellulose) has been a subject of continuous research as a new material. Plant-derived cellulose and bacterial cellulose show significant structural differences. That is, bacterial cellulose derived from microorganisms is composed of ribbon-like bundles, whereas plant-derived cellulose is composed of bundles of microfibrils.

In particular, cellulose produced by acetic acid bacteria is produced in a pure state that does not contain lignin or hemicellulose, unlike plant-derived cellulose, which is composed of bundles of microfibrils. As a result of research on productivity improvement by strain improvement, genetic manipulation, establishment of culture conditions, etc., it can be used as high-tech materials such as high-strength industrial materials, composite fibers, medical materials, and enzyme immobilization.

In addition, cellulose produced by microorganisms has a very high biocompatibility and is used as a medical material such as artificial cartilage, artificial blood vessels, dressing agents, burn treatment agents, etc. Representatively, Dermafill (Georgia, USA) products are widely used. In addition, it is less irritating than hydrogel mask packs developed as a substitute for non-woven fabric sheets as a beauty material and is composed of nano-size, so it has a larger surface area and higher tensile strength than general non-woven fabric masks, so it has excellent skin adhesion and has more than 10 times higher wettability. It can increase skin absorption rate.

Another key property of cellulose produced by microorganisms is its good biodegradability. Cellulose produced by microorganisms is a promising polymer material from an environmental point of view because it is biodegraded in soil within one month. If low-cost mass production is possible, the ability as an eco-friendly polymer material will be very excellent.

However, the reason why the various functionalities of cellulose produced by microorganisms have not been fully utilized until now is because the culture technology for mass production has not been properly established, and the cost of raw materials is high because commercial complex media are used as raw materials for production. .

Currently, strains producing cellulose include Acetobacter sp., Agrobacterium sp., Rhizobium sp., Pseudomonas sp., and Sarcina sp. .), especially among the Acetobacter sp., Acetobacter xylium, A. pasteurinanus and Acetobacter hansenii ( A. hansenii ) are well known. .

Cellulose-producing strains have recently been reclassified molecularly and phylogenetically using 16S rRNA sequences. It has been reported that the strain producing high-purity cellulose is morphologically an enterococci and secretes cellulose from the cell body as a bacterium with strong acid resistance. Glucose is the most representative carbon source used in conventional cellulose production, and among natural materials, persimmon juice and persimmon vinegar, apple juice, grape juice, beer waste liquid, and coconut by-products have been used as other materials.

Accordingly, the present inventors have prepared a biogel using Komagataeibacter intermedius NY-Gah 150-2 strain or Gluconacetobacter europaeus NY-Gch 187-1 strain isolated from natural vinegar. It can be produced in high yield, and it was confirmed that the produced biogel has excellent physical properties.

Accordingly, an object of the present invention is to provide a Comagateibacter intermedius NY-Gah 150-2 strain having excellent biogel production ability.

Another object of the present invention is to provide a composition for producing a biogel containing the Comagataybacter intermedius NY-Gah 150-2 strain.

Another object of the present invention is to provide a biogel production method comprising a culturing step of culturing the Comagataybacter intermedius NY-Gah 150-2 strain.

Another object of the present invention is to provide a Gluconacetobacter europaus NY-Gch 187-1 strain having excellent biogel production ability.

Another object of the present invention is to provide a composition for producing a biogel containing the Gluconacetobacter europaus NY-Gch 187-1 strain.

Another object of the present invention is to provide a biogel production method comprising a culturing step of culturing the Gluconacetobacter europaus NY-Gch 187-1 strain.

Another object of the present invention is to provide a food composition comprising a biogel produced by culturing the Gluconacetobacter europaus NY-Gch 187-1 strain.

Another object of the present invention relates to the biogel production use of Comagataybacter intermedius NY-Gah 150-2 strain or Gluconacetobacter europaus NY-Gch 187-1 strain.

The present invention relates to Komagataeibacter intermedius NY-Gah 150-2 strain or Gluconacetobacter europaeus NY-Gch 187-1 strain and a biogel production method using the same, Using the strain according to the present invention, biogel having excellent physical properties can be produced in high yield.

The present inventors secured about 920 single colonies selected from 6 natural vinegars and obtained the final 10 strains with high biogel-producing ability from these strains. Gah 150-2 strain and Gluconacetobacter europaus NY-Gch 187-1 strain, which are most industrially used strains, Komagataeibacter xylinus ( Komagataeibacter xylinus , KACC12367), Gluconacetobacter genus ( Gluconacetobacter sp , KACC91555P), it was confirmed that the gel-forming ability and the strength, production amount, and uniformity of the resulting strain were excellent.

Hereinafter, the present invention will be described in more detail.

One aspect of the present invention is a Comagateibacter intermedius NY-Gah 150-2 strain having excellent biogel production ability.

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In the present invention, the Comagataybacter intermedius NY-Gah 150-2 strain may be deposited under accession number KCTC 14296BP.

Another aspect of the present invention is a composition for producing a biogel comprising a Comagataybacter intermedius NY-Gah 150-2 strain.

The gel prepared using the biogel production composition has particularly excellent tensile strength and is highly likely to be used as a mask sheet for absorbing the cosmetic composition into the skin.

Another aspect of the present invention is a biogel production method comprising a culturing step of culturing the Comagateibacter intermedius NY-Gah 150-2 strain.

In the present invention, the culturing step may be carried out by static culture at 25 ° C to 37 ° C conditions for 3 to 10 days using a gel-forming medium with a strain culture inoculation concentration of 0.5 to 2.0% (v / v). And, preferably, the inoculation concentration of the culture solution is 0.8 to 1,5% (v / v), and it may be carried out at 25 ° C to 33 ° C for 3 to 4 days.

In the present invention, the inoculation concentration of the culture medium relates to the concentration of inoculating the strain in the gel production medium to produce the gel, and was based on using a strain culture medium having an OD of 600 nm 0.5.

The culturing step is performed at 25°C to 30°C, 28°C to 37°C or 28°C to 33°C, for example, 28°C to 30°C, but is not limited thereto.

In one embodiment of the present invention, it was confirmed that the gel production medium was most efficient when inoculated at a culture medium inoculation concentration of 1% (v/v) and performed at 30° C. for 4 days.

In one embodiment of the present invention, the biogel produced from the NY-Gah 150-2 strain had a tensile strength 3 to 10 times higher than most gels and a yield more than 2 times higher.

Another aspect of the present invention is a Gluconacetobacter europaus NY-Gch 187-1 strain having excellent biogel production ability.

In the present invention, the Gluconacetobacter europaus NY-Gch 187-1 (187) strain may be deposited under accession number KCTC 14297BP.

Another aspect of the present invention is a composition for producing a biogel comprising a Gluconacetobacter europaus NY-Gch 187-1 strain.

The gel prepared using the composition for producing biogel not only has excellent tensile strength, but also can be used for food production because it is prepared by strains that can be used for food.

The composition for producing biogel may additionally include Lactobacillus hilgardii NY-Gbo 5-1-3 strain.

In the present invention, the Lactobacillus Hilgardi NY-Gbo 5-1-3 strain may be deposited under accession number KCTC 14295BP.

Another aspect of the present invention is a biogel production method comprising a culturing step of culturing the Gluconacetobacter europaus NY-Gch 187-1 strain.

In the present invention, the culturing step may be carried out by static culture at 25 ° C to 37 ° C conditions for 3 to 10 days using a gel-forming medium with a strain culture inoculation concentration of 0.5 to 2.0% (v / v). And, preferably, the inoculation concentration of the culture solution is 0.8 to 1,5% (v / v), and it may be carried out at 25 ° C to 33 ° C for 3 to 4 days.

The culturing step is performed at 25°C to 30°C, 28°C to 37°C or 28°C to 33°C, for example, 28°C to 30°C, but is not limited thereto.

In one embodiment of the present invention, it was confirmed that the gel production medium was most efficient when inoculated at a culture medium inoculation concentration of 1% (v/v) and performed at 30° C. for 4 days.

Considering that most of the common biogel-producing strains are derived from inedible strains, NY-Gch 187-1 is an edible strain and has a higher biogel-producing ability than the tested strain, so it is advantageous to use for food.

The culturing step may be performed by additionally including the Lactobacillus hilgardi NY-Gbo 5-1-3 strain. If the culturing step is performed by additionally including strain Lactobacillus hilgardi NY-Gbo 5-1-3, Gluconacetobacter europaus NY-Gch 187-1 strain: Lactobacillus hilgardi NY-Gbo 5-1 -3 The mixed volume ratio of strains may be 1.0:0.2 to 1.0:1.8, 1.0:0.2 to 1.0:1.5, 1.0:0.2 to 1.0:1.2, 1.0:0.8 to 1.0:1.8 or 1.0:0.8 to 1.0:1.5 , For example, it may be 1.0:0.8 to 1.0:1.2, but is not limited thereto.

In one embodiment of the present invention, the culturing step is the whole culture including the culture medium of the Gluconacetobacter europaus NY-Gch 187-1 strain and the Lactobacillus hilgardi NY-Gbo 5-1-3 strain It was performed by inoculating the gel production medium by mixing each 0.5% (v / v) of the contrast.

Another aspect of the present invention is a food composition comprising a biogel produced by culturing the Gluconacetobacter europaus NY-Gch 187-1 strain.

The food composition may include a biogel produced by culturing a mixture of Gluconacetobacter europaus NY-Gch 187-1 strain and Lactobacillus hilgardi NY-Gbo 5-1-3 strain.

The mixed volume ratio of Gluconacetobacter europaus NY-Gch 187-1 strain: Lactobacillus hilgardi NY-Gbo 5-1-3 strain was 1.0:0.2 to 1.0:1.8, 1.0:0.2 to 1.0:1.5, 1.0: It may be 0.2 to 1.0:1.2, 1.0:0.8 to 1.0:1.8 or 1.0:0.8 to 1.0:1.5, for example, it may be 1.0:0.8 to 1.0:1.2, but is not limited thereto.

When the food composition of the present invention is used as a food additive, the food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods. In general, the food composition of the present invention can be added in an amount of 15% by weight or less, preferably 10% by weight or less, based on the raw material during production of food or beverage.

There is no particular limitation on the type of food. Examples of foods to which the substance can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, There are alcoholic beverages, vitamin complexes, and the like, and includes all foods in a conventional sense.

The beverage may contain various flavoring agents or natural carbohydrates as additional ingredients. The aforementioned natural carbohydrates may include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, natural sweeteners such as dextrin and cyclodextrin, and synthetic sweeteners such as saccharin and aspartame. . The ratio of the natural carbohydrates can be appropriately determined by a person skilled in the art.

In addition to the above, the food composition of the present invention includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol , carbonation agents used in carbonated beverages, and the like. In addition, the food composition of the present invention may contain fruit flesh for preparing natural fruit juice, fruit juice beverages and vegetable beverages. These components may be used independently or in combination. The ratio of these additives can also be appropriately selected by those skilled in the art.

The present invention relates to Komagataeibacter intermedius NY-Gah 150-2 strain or Gluconacetobacter europaeus NY-Gch 187-1 strain, and a biogel production method using the same. , Since biogel with excellent physical properties can be produced in high yield by using the above strain, it can be used as a pure biogel for medical, beauty, food, electrical and electronic fields or new functional materials for food, depending on the purpose, for industrial use and can be used for research.

1 is a photograph showing six kinds of natural vinegar-derived biogels according to an embodiment of the present invention.
Figure 2 is a photograph showing the formation of 22 colonies and gels with high gel-producing ability among the primary selection strains according to an embodiment of the present invention.
3 is a photograph showing the formation of colonies and gel formation of 10 strains having high gel-producing ability and high physical properties of the gel among the primary selection strains according to an embodiment of the present invention.
4 is a photograph showing the formation of 19 colonies and gels with high gel-producing ability among secondary selection strains according to an embodiment of the present invention.
5 is a photograph showing the results of large-capacity gel production of primary and secondary selection strains according to an embodiment of the present invention.
6 is a phylogenetic diagram according to comparison between 16S rDNA gene sequences of related species prepared according to the neighbor-joining method and the NY-Gah 150-2 strain of Komagataeibacter intermedius .
Figure 7 is a phylogenetic diagram according to comparison between 16S rDNA gene sequences of related species prepared according to the Gluconacetobacter europaeus NY-Gch 187-1 strain and the neighbor-joining method.
Figure 8a is a photograph showing the production result of the biogel produced using the NY-Gah 150-2 strain.
Figure 8b is a graph showing the tensile strength of the biogel produced using the NY-Gah 150-2 strain.
9 is a photograph showing the result of biogel produced by mixing and culturing NY-Gch 187-1 strain and Lactobacillus hilgardii NY-Gbo 5-1-3 strain.

Hereinafter, the present invention will be described in more detail by the following examples. However, these examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Throughout this specification, "%" used to indicate the concentration of a particular substance is (weight/weight)% for solids/solids, (weight/volume)% for solids/liquids, and liquid/liquid is (volume/volume) %.

Reagents used in the analysis were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).

Example 1: Preparation and confirmation of natural vinegar

Six types of natural vinegar were prepared using beets, green tea, fresh vinegar, gujippong, figs and green apples according to a conventional vinegar manufacturing method. As shown in FIG. 1, natural biogels were obtained from each vinegar, and physical properties were confirmed for 6 types of natural biogels.

Physical properties of 6 natural biogels vinegar tensile strength
(g/cm ² ) thickness
(mm) Shout
(Springiness) Gumminess (g) Chewiness (g) Cohesiveness beat 5,320 1.69 0.35 1,543 538 0.28 FIG 346 1.52 0.41 160 66 0.46 green tea 2,105 3.44 0.36 774 279 0.37 green apple 5,180 0.32 0.52 1,684 877 0.31 Gujippong 1,016 2.5 0.42 366 154 0.35 fresh grass 1,740 2.88 0.61 405 247 0.21

As a result of examining the tensile strength and thickness of six kinds of natural vinegar-derived biogel, the gel made from beets and green apples had the highest tensile strength of more than 5000 g/cm ² , and the green tea-derived biogel had the thickest thickness.

Example 2: Cultivation and selection of strains

2-1. 1st selection: stock solution-derived strains

After suspending 1 ml of the vinegar stock solution of Example 1 in sterile physiological saline to 10 ^-1 to 10 ^-6 , 100 μL each was plated on H & S agar medium, which is a gel forming medium, and cultured at 30 ° C. for 7 days.

The composition of H&S broth was glucose (glucose) 2%, yeast extract (Yeast extract) (Yeast extract) (Yeast extract) (0.5%), polypeptone (polypeptone) 0.5%, H & S agar was added to the H & S broth agar 2%. The pH was adjusted to 6.0 with 0.657% sodium phosphate and 0.15% citric acid.

The selected colonies were pure isolated in the same medium and cultured for 3 generations, and then 157 single colonies were obtained.

As shown in FIG. 2, 22 species of bacteria producing biogel were selected. In order to investigate the physical properties of the biogel produced using 22 strains, the weight, dry weight, moisture content, gel uniformity, and tensile strength of the resulting gel were investigated after generating the gel based on 7.5 mL medium.

Results of investigation of physical properties of biogels produced by 22 strains vinegar number weight (g) dry weight (g) Moisture content (g) gel uniformity
(density) Tensile strength (g/cm ² ) beat 5-1 1.27 0.05 1.22 165 645 5-1-1 1.19 0.06 1.13 145 782 10 0.84 0.04 0.8 127 489 14-1-2 1.08 0.04 1.04 143 724 Gujippong 9-1 1.44 0.05 1.39 163 513 9-2 0.56 0.02 0.54 136 343 10 0.71 0.04 0.67 139 319 23-1 0.84 0.04 0.8 135 434 23-2 1.45 0.03 1.42 143 518 24-1 0.98 0.03 0.95 146 657 24-2 0.84 0.03 0.81 154 340 27-1 1.12 0.04 1.08 157 480 27-2 1.1 0.04 1.06 183 493 28 1.08 0.04 1.04 152 348 35 1.18 0.04 1.14 155 361 45-1 1.12 0.05 1.07 151 412 49-1 1.09 0.07 1.02 174 671 49-2 1.25 0.07 1.18 144 456 59-1 0.4 0.02 0.38 124 347 59-2 1.36 0.04 1.32 164 649 FIG One 1.33 0.06 1.27 183 648 2 1.19 0.05 1.14 163 699

The physical properties of the gel produced using a total of 22 strains with high biogel production ability were investigated. Physical properties were performed by examining tensile strength, moisture content (weight after drying at 80°C for 8 hours), weight of the resulting biogel, and uniformity (densitometry, NIH-densitometry).

Specifically, tensile strength was analyzed using a texture analyzer (TA.XT plus, Stable Micro Systems, Surrey, UK). The gel was placed between aluminum probe supports (100 mm in width and 100 mm in length) with a hole of 6 mm in the middle, and both sides were fixed, and the height was set to 25 mm. After that, the rod-shaped probe (diameter 5 mm) was lowered at a speed of 1 mm/min until it could pass through the biogel sample. The degree of gel formation is tensile strength (g/cm ² ) 0 mm (-), 350 mm (+), 350-500 mm (++), 500-650 mm (+++), 650 mm or more (+++) +) to select strains with excellent biogel production ability.

The amount of biogel produced was measured according to a known method. That is, the biogel formed in the form of a membrane was thoroughly washed with distilled water, immersed in a 0.25 N NaOH solution, allowed to stand for one day, and neutralized by adding an equal amount of 0.25 N acetic acid. Again, it was sufficiently washed with distilled water to remove medium components, cells, and impurities, and then dried in an 80° C. dry oven to measure the constant weight.

The uniformity of the resulting gel was quantified using the NIH densitometry program of the densitometry instrument.

As shown in Table 3 and FIG. 3, a total of 10 gel-producing strains based on a gel weight of 1 g or more, a tensile strength of 500 g / cm ² , a water content of 1 g or more, and a homogeneity of 140 or more when using a density meter was selected

Physical properties of biogel produced using 10 strains with high gel-forming ability vinegar number weight (g) dry weight (g) Moisture content (g) gel uniformity
(density) tensile strength
(g/cm ² ) beat 5-1 1.27 0.05 1.22 165 645 5-1-1 1.19 0.06 1.13 145 782 14-1-2 1.08 0.04 1.04 143 724 Gujippong 9-1 1.44 0.05 1.39 163 513 23-2 1.45 0.03 1.42 143 518 24-1 0.98 0.03 0.95 146 657 49-1 1.09 0.07 1.02 174 671 59-2 1.36 0.04 1.32 164 649 FIG One 1.33 0.06 1.27 183 648 2 1.19 0.05 1.14 163 699

The 10 strains finally selected for gel characteristics were inoculated with 1% (0.1 mL v/v) of a bacterial culture medium showing an OD 600 nm of 0.5 by adding 125 mL H&S liquid medium to a 250 ml Erlenmeyer flask with a large capacity and inoculated for 30 days for 7 days. Cultivated in a stationary incubator.

2-2. Secondary selection: Strains derived from stock culture using H&S medium for gel production

After suspending 1 ml of the vinegar stock solution of Example 1 in sterile physiological saline to 10 ^-1 to 10 ^-6 , culturing in 10 mL H&S liquid medium using a tube (falcon tube, 50 mL), absorbance at OD 600 nm was measured, and 1% (0.1 mL v/v) of the bacterial culture showing 0.5 was inoculated into the H&S liquid medium and incubated statically in a 30° C. incubator for 7 days.

100 μL of this was again smeared on H & S agar medium, which is a gel production medium, and statically cultured at 30 ° C. for 3 to 4 days to collect 763 species. After pure separation in the same medium and subculture for three generations, a single colony was taken and cultured in H&S liquid medium (5 mL) for use.

Among a total of 763 strains, 50 strains showing gel-producing ability were selected, and the physical properties of the gel produced using them were investigated. It was confirmed that the density of the gel derived from the first selection strain was generally higher than that of the second selection. As shown in Table 4 and FIG. 4, a total of 19 gel-producing strains based on a gel weight of 1 g or more, a tensile strength of 500 g / cm ² , a water content of 1 g or more, and a homogeneity of 100 or more when using a density meter selected.

Physical properties of biogel produced by 19 strains vinegar number weight (g) dry weight (g) Moisture content (g) uniformity tensile strength
(g/cm ² ) fresh grass 20-1 1.01 0.05 0.96 125 527 20-2 1.14 0.06 1.09 155 544 111-2 0.83 0.04 0.79 83 3535 111-3 1.16 0.06 1.10 168 537 151-1 0.37 0.02 0.35 190 779 151-2 0.63 0.03 0.60 125 505 187-1 0.27 0.01 0.25 193 1110 187-2 0.43 0.02 0.41 173 1855 green apple 38-1 1.30 0.06 1.23 130 918 51-1 1.71 0.09 1.63 143 1480 63-2 1.14 0.06 1.08 133 1035 72-1 1.30 0.07 1.24 123 1310 150-2 1.81 0.09 1.72 118 951 Gujippong 31-3 1.54 0.06 1.48 166 492 59-2 1.38 0.06 1.32 168 486 FIG 3-2 1.22 0.05 1.17 177 436 71-2 1.55 0.05 1.5 159 497 75-1 1.37 0.05 1.32 169 630 75-2 1.39 0.06 1.33 168 482

Example 3: Confirmation of large-capacity gel production ability of 29 strains and investigation of physical properties

Using 29 strains (10 strains for the first, 19 strains for the second) selected in Example 2, 125 mL H&S liquid large-capacity medium in a 250 mL Erlenmeyer flask was cultured at 30 ° C for 4 days. , after performing the gel formation, their physical properties were investigated.

When selecting strains with high performance of the final biogel at each stage, tensile strength of 500 g/cm ² , weight of 1 g or more (7.5 mL), and moisture content: 1 g or more (7.5 mL) were used.

As a control group, biogel production and gel properties were compared using two strains of Komagataeibacter xylinus ( Komagataeibacter xylinus , KACC12367) and Gluconacetobacter sp. (KACC91555P).

Physical properties of biogels produced with 29 strains
number weight (g) dry weight (g) moisture content
(g) Tensile strength (g/cm ² ) uniformity
(density) Specified strain 12367 14.67 0.31 14.36 381.0 142 91555p 11.35 0.26 11.09 84.0 115 Primary beat 5-1 10.76 0.56 10.20 695.0 118 5-1-1 12.38 0.99 11.39 1,410.0 138 14-1-2 11.59 0.75 10.84 969.0 125 Gujippong 9-1 15.41 0.82 14.59 656.0 159 23-2 18.95 2.00 16.95 493.0 165 24-1 12.75 0.77 11.98 180.0 155 49-1 10.34 0.69 9.65 78.3 143 59-2 13.94 1.27 12.67 716.0 161 FIG One 18.72 1.05 17.67 1,010.0 183 2 19.56 0.95 18.61 1,200.0 163 Secondary fresh grass 20-1 16.31 0.84 15.47 719.0 155 20-2 13.62 0.67 12.95 424.0 148 111-2 10.14 0.46 9.68 624.0 159 111-3 16.11 0.85 15.26 528.0 144 151-1 8.47 0.58 7.89 1,110.0 139 151-2 12.07 0.45 11.62 822.0 146 187-1 11.2 0.69 10.51 1,160.0 146 187-2 11.35 0.64 10.71 971.0 141 green apple 38-1 15.94 0.98 14.96 1,370.0 123 51-1 17.66 0.96 16.70 775.0 146 63-2 14.33 0.88 13.45 653.0 138 72-1 19.41 0.81 18.60 1,440.0 140 150-2 21.13 1.23 19.90 2,390.0 133 Gujippong 31-3 13.2 1.7 11.50 1,010.0 166 59-2 11.51 1.18 10.33 494.0 168 FIG 3-2 17.61 1.00 16.61 860.0 177 71-2 14.78 0.76 14.02 860.0 159 75-1 14.17 0.91 13.26 613.0 169 75-2 17.13 1.09 16.04 411.0 168

As can be seen in Table 5 and FIG. 5, as a result of investigation of the physical properties of biogels produced from 29 strains with high gel-producing ability, beet 5-1-1, Gujippong 31-3, Fig 1, Fig 2, Shinsegae 111- 2, Shinsangcho 187-1, Cheongsagwa 38-1, Cheongsagwa 72-1, Cheongsagwa 150-2, and Cheongsagwa 51-1 were selected. All 10 selected strains showed higher tensile strength and uniformity than the tested strains.

Biogel physical properties of 10 selected strains number weight (g) dry weight (g) moisture content
(g) Tensile strength (g/cm ² ) uniformity
(density) Specified strain 12367 14.67 0.31 14.36 381 142 91555p 11.35 0.26 11.09 84 115 Primary beat 5-1-1 12.38 0.99 11.39 1,410 138 FIG One 18.72 1.05 17.67 1,010 183 2 19.56 0.95 18.61 1,200 163 Secondary fresh grass 111-2 10.14 0.46 9.68 624 159 187-1 11.20 0.69 10.51 1,160 146 green apple 38-1 15.94 0.98 14.96 1,370 123 51-1 17.66 0.96 16.70 775 146 72-1 19.41 0.81 18.60 1,440 140 150-2 21.13 1.23 19.90 2,390 133 Gujippong 31-3 13.20 1.70 11.50 1,010 166

Example 4: Identification of 10 biogel producing strains

Beet 5-1-1, Gujippong 31-3, Fig 1, Fig 2, Sinseoncho 111-2, Sinseoncho 187-1, Green Apple 38-1, Green Apple 72-1, Green Apple 150-2, The 16S rRNA gene sequence of green apple 51-1 was analyzed. After DNA was extracted from the cells, their 16S rRNA gene was PCR amplified using universal primers, 27F and 1492R. The amplified gene products were sequenced using universal sequencing primers, 785F and 907R primers, commissioned by Macrogen.

Primers for PCR and sequencing to identify 10 biogel-producing strains sequence number designation Sequence (5'-3') One 785 F primer for 16s rRNA sequencing GGA TTA GAT ACC CTG GTA 2 907 R primer for 16s rRNA sequencing CCG TCA ATT CMT TTR AGT TT 3 27 F primer for 16s rRNA PCR AGA GTT TGA TCM TGG CTC AG 4 1492 R primer for 16s rRNA PCR TAC GGY TAC CTT GTT ACG ACT T

The 16S rRNA gene sequence of the strain was compared with the known 16S rRNA gene sequence in the NCBI nucleotide sequence database (www.ncbi.nlm.nih.gov./blast/) by BLAST search.

Identification results of 10 biogel-producing bacteria and information permitted by the Ministry of Food and Drug Safety NO Strain no. denomination Accession
no. Identification Identity (%) food raw material
item number One beat 5-1-1 BO5-1-1 NY-Gbo
5-1-1 NR_114695.1 Komagataeibacter
sucrofermentans 99 Not applicable 2 Gujippong (HS) 31-3 GH31-3 NY-Ggh31-3 LC108743.1 Komagataeibacter
rhaeticus 99 Not applicable 3 fig 1 MO1 NY-Gmo 1 NR_026435.1 Komagataeibacter intermedius 99 Not applicable 4 fig 2 MO2 NY-Gmo 2 NR_026435.1 Komagataeibacter intermedius 99 Not applicable 5 Sinseoncho 111-2 111-2 NY-Gch111-2 NR_026513.1 Gluconacetobacter
europaeus 99 edible 6 Sinseoncho 187-1 187-1 NY-Gch187-1 NR_026513.1 Gluconacetobacter
europaeus 99 edible 7 Green Apple 38-1 C38-1 NY-Gah38-1 NR_114695.1 Gluconacetobacter
sucrofermentans 99 Not applicable 8 Green Apple 72-1 C72-1 NY-Gah72-1 NR_114695.1 Gluconacetobacter
sucrofermentans 99 Not applicable 9 Green Apple 150-2 C150-2 NY-Gah 150-2 NR_026435.1 Gluconacetobacter intermedius 99 Not applicable 10 Green Apple 51-1 C51-1 NY-Gah51-1 NR_114695.1 Gluconacetobacter
sucrofermentans 99 Not applicable

As can be seen in Table 8, the 16S rRNA gene sequences of 10 species are respectively Komagataeibacter sucrofermentans , Komagataeibacter rhaeticus , and Komagataeibacter intermedius ( Komagataeibacter intermedius ) , Gluconacetobacter europaus ( Gluconacetobacter europaeus ) , Gluconacetobacter sucrofermentans ( Gluconacetobacter sucrofermentans ) , Gluconacetobacter intermedius ( Gluconacetobacter intermedius ) It was confirmed that they had 99% homology.

Example 5: Identification of strains with the highest biogel ability, genetic sequence and phylogenetic tree analysis

Multiple sequence alignment was performed using the known Clustal X. Gaps at the 5' and 3' ends were edited again through further analysis and are shown in Table 9 below.

sequence number designation order 5 Gluconacetobacter intermedius (NY-Gah 150-2) 16S rRNA GGGGGGGGTCAATTTTATAAGAGGGGAATCTTTAGAGTTTGGAATCAGGCTCAGAGCGAACGCTGGCGGCATGCTTAACACATGCAAGTCGCACGAACCTTTCGGGGTTAGTGGCGGACGGGTGAGTAACGCGTAGGGATCTGTCCACGGGTGGGGGGATAACTTTGGGAAACTGAAGCTAATACCGCATGACACCTGAGGGTCAAAGGCGCAAGTCGCCTGTGGAGGAACCTGCGTTCGATT AGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGATGATCGATAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGTGTGAAGAAGGTTTTCGGATTGTAAAGCACTTTCAGCGGGGACGATGATGACGGTACCCGCAGAAGAAGCCCCGGCTAACT TCGTGCCAGCAGCCGCGGTAATACGAAGGGGGCAAGCGTTGCTCGGAATGACTGGGCGTAAAGGGCGCGTAGGCGGTTGACACAGTCAGATGTGAAATTCCTGGGCTTAACCTGGGGGCTGCATTTGATACGTGGCGACTAGAGTGTGAGAGGGTTGTGGAATTCCCAGTGTAGAGGTGAAATTCGTAGATATTGGGAAGAACACCGGTGGCGAAGGCCAACCTGGCTCATGACTGAC GCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATGTGTGCTGGATGTTGGGTGACTTTGTCATTCAGTGTCGTAGTTAACGCGATAAGCACACCGCCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGGGCTTGACATGC GGAGGCCGTGTCCAGAGATGGGCATTTCTCGCAAGAGACCTCCAGCACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGCCTTTAGTTGCCAGCACGTCTGGGTGGGCACTCTAAAGGAACTGCCGGTGACAGCCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGTCCTGGGCTACACACACGTGCTA CAATGGCGGTGACAGTGGGAAGCCAGGTGGTGACACCGAGCCGATCTCAAAAAGCCGTCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGGTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGTTTGACCTTAAGCCGGTGAGGCGAACCGCAAGGACGCAGCCGACCACGGTCGG GTCAGCGACTGGGGTGAAGTCGTACAGGGTAAAACCCGAAAAACTGTGGCCTCCCGCATCTATCATCACA 6 Gluconacetobacter europaeus ( NY-Gch 187-1) 16S rRNA CATGCTTAACACATGCAAGTCGCACGAACCTTTCGGGGTTAGTGGCGGACGGGTGAGTAACGCGTAGGGATCTGTCCATGGGTGGGGGGATAACTTTGGGAAACTGAAGCTAATACCGCATGACACCTGAGGGTCAAAGGCGCAAGTCGCCTGTGGAGGAACCTGCGTTCGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGATGATCGATAGCTGGTCTGAGAGGATGATCAGCC ACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCAATGCCGCGTGTGTGAAGAAGGTTTTCGGATTGTAAAGCACTTTCAGCGGGGACGATGATGACGGTACCCGCAGAAGAAGCCCCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGGGCAAGCGTTGCTCGGAATGACTGGGCGTAAAGGC GCGTAGGCGGTTGACACAGTCAGATGTGAAATTCCTGGGCTTAACCTGGGGGCTGCATTTGATACGTGGCGACTAGAGTGTGAGAGGGTTGTGGAATTCCCAGTGTAGAGGTGAAATTCGTAGATATTGGGAAGAACACCGGTGGCGAAGGCGGCAACCTGGCTCATGACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATG TGTGCTGGATGTTGGGTGACTTTGTCATTCAGTGTCGTAGTTAACGCGATAAGCACACCGCCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGGGCTTGACATGCGGAGGCCGTGTCCAGAGATGGGCATTTCTCGCAAGAGACCTCCAGCACAGGTGCTGCATGGCTGTC GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGCCTTTAGTTGCCATCACGTTTGGGTGGGCACTCTAAAGGAACTGCCGGTGACAGCCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATGTCCTGGGCTACACACACACGTGCTACAATGGCGGTGACAGTGGGAAGCCAGGTAGCGATACCGAGCCGATCTCATAAAGCCGTCTCAGTTC GGATTGCACTCTGCAACTCGAGTGCATGAAGGTGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCGCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTTTGACCTTAAGCCGGTGAGGCGAACCGCAAGGACGCAGCCGACCACGGTCGGGTC

In particular, the phylogenetic tree of two strains with distinct characteristics, C150-2 (NY-Gah 150-2) and 187-1 (NY-Gch 187-1), was analyzed using the neighbor-joining method using the MEGA 2.1.0 package. (neighbor-joining method) was manufactured as shown in FIGS. 6 and 7.

The results of the identification of the biogel progenitor strains NY-Gah 150-2 and NY-Gch 187-1 and whether they are acceptable for food are shown in Tables 10 and 11 below.

Identification result of biogel probiotic strain NY-Gah 150-2 and information permitted by the Ministry of Food and Drug Safety explanation Aceession# Pct (%) allowed by the Food and Drug Administration Komagataeibacter intermedius strain TF2 16S ribosomal RNA, partial sequence NR_026435.1 99 corresponding
doesn't exist Komagataeibacter sucrofermentans strain BPR 2001 16S ribosomal RNA gene, partial sequence NR_114695.1 99 Komagataeibacter oboediens strain LTH 2460 16S ribosomal RNA gene, partial sequence NR_041295.1 99 Komagataeibacter europaeus strain DES11 16S ribosomal RNA gene, partial sequence NR_026513.1 99 Komagataeibacter oboediens strain LTH2460 16S ribosomal RNA, partial sequence NR_114683.1 99 Komagataeibacter europaeus strain DSM 6160 16S ribosomal RNA gene, partial sequence NR_112539.1 99 Komagataeibacter sucrofermentans strain JCM 9730 16S ribosomal RNA gene, partial sequence NR_112538.1 99 Komagataeibacter nataicola strain LMG 1536 16S ribosomal RNA gene, partial sequence NR_041012.1 99 Komagataeibacter xylinus strain NCIB 11664 16S ribosomal RNA gene, partial sequence NR_036787.1 99 Komagataeibacter swingsii strain DST GL01 16S ribosomal RNA gene, partial sequence NR_042762.1 99 Lactobacillus fuchuensis strain LMG 21669 16S ribosomal RNA gene, partial NR_104976.1 98

Identification result of biogel probiotic strain NY-Gch 187-1 and information permitted by the Ministry of Food and Drug Safety explanation Aceession# Pct (%) allowed by the Food and Drug Administration Komagataeibacter europaeus strain DES11 16S ribosomal RNA gene, partial sequence NR_026513.1 99 It's B
003350 Komagataeibacter xylinus strain NCIB 11664 16S ribosomal RNA gene, partial sequence NR_036787.1 99 Komagataeibacter intermedius strain TF2 16S ribosomal RNA, partial sequence NR_026435.1 99 Komagataeibacter swingsii strain DST GL01 16S ribosomal RNA gene, partial sequence NR_042762.1 99 Komagataeibacter sucrofermentans strain BPR 2001 16S ribosomal RNA gene, partial sequence NR_114695.1 99 Komagataeibacter saccharivorans strain LMG 1582 16S ribosomal RNA, partial sequence NR_108135.1 99 Komagataeibacter oboediens strain LTH2460 16S ribosomal RNA, partial sequence NR_114683.1 99 Komagataeibacter medellinensis NBRC 3288 DNA, complete genome AP012159.1 99 Komagataeibacter medellinensis strain NBRC 3288 16S ribosomal RNA, partial sequence NR_074338.1 99 Komagataeibacter europaeus strain DSM 6160 16S ribosomal RNA gene, partial sequence NR_112539.1 99 Lactobacillus fuchuensis strain LMG 21669 16S ribosomal RNA gene, partial NR_104976.1 98

Example 6: Confirmation of Biogel Producing Ability of the Two Finally Selected Strains

For the two finally selected strains, biogel production was performed in H&S liquid medium at 30 ° C for 3 to 4 days to confirm the biogel production ability.

As can be seen in Figure 8a, NY-Gah 150-2 has the highest gel-forming ability among the 920 strains selected, the tensile strength and density of the biogel produced are remarkable, and the gel-forming ability is the highest at 47 g. In particular, as can be seen in Figure 8b, the tensile strength was remarkably superior to that of biogels derived from other strains.

The NY-Gah 150-2 strain suggested the possibility of use as a mask pack or functional patch for industrial use. On the other hand, since it is not an edible strain, it cannot be used as food, so it was necessary to select a strain for use as a biogel for food, so the NY-Gch 187-1 strain was additionally selected.

Considering that most of the general biogel-producing strains are derived from inedible strains, NY-Gch 187-1, which was additionally selected, showed a gel-producing ability of about 70% compared to NY-Gah 150-2 as an edible strain.

Since the production ability of NY-Gch 187-1 is significantly lower than that of NY-Gah 150-2, it is necessary to supplement the NY-Gch 187-1 strain and accession number KCTC 14295BP to apply the mixed strain utilization method. The Lactobacillus hilgardii NY-Gbo 5-1-3 strain was mixed with 0.5% (v/v) of the entire culture including the medium, and inoculated into the medium and cultured.

The strain Lactobacillus Hilgardi NY-Gbo 5-1-3 was selected and identified for its ability to produce GABA from vinegar, and Lactobacillus Hilgardi is generally known to have a high ability to produce GABA.

As can be seen in Figure 9, compared to the case of culturing with a single NY-Gch 187-1 strain, Lactobacillus hilgardi In the case of mixed culture with NY-Gbo 5-1-3, it was confirmed that the gel production ability was increased by 2.1 times to 67 g, so that it could be applied to the production and utilization of edible gel. This corresponds to an activity 1.5 times higher than the gel production using only a single strain, NY-Gah 150-2, and it can be confirmed that the gel production ability increased through their mixed culture.

Comagateibacter intermedius NY-Gah 150-2, which has generally high efficacy in terms of such strong tensile strength and gel-forming ability, is used for mask packs or industrial purposes, Gluconacetobacter europaus NY-Gch 187-1 is Lactobacillus hilgardi It is believed that each can be used differently for the purpose of producing biogel for food by mixing with NY-Gbo 5-1-3.

Therefore, as a pure biogel using the above two strains, it can be applied in various fields such as medical, beauty, food, and electrical and electronic fields, as well as develop functional edible gel in food to maximize the applicability of the industry in the future. It is considered that it can be used as a high-tech new material.

Korea Research Institute of Bioscience and Biotechnology KCTC14295BP 20200903 Korea Research Institute of Bioscience and Biotechnology KCTC14296BP 20200903 Korea Research Institute of Bioscience and Biotechnology KCTC14297BP 20200903

<110> INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY <120> The strain of Komagataeibacter intermedius NY-Gah 150-2 or the strain of Gluconacetobacter europaeus NY-Gch 187-1, and method for producing cellulose using the same <130> PN200264 <160> 6 <170> KoPatentIn 3.0 <210> 1 <211> 18 <212> DNA <213> artificial sequence <220> <223> 785 F primer <400> 1 ggattagata ccctggta 18 <210> 2 <211> 20 <212> DNA <213> artificial sequence <220> <223> 907 R primer <400> 2 ccgtcaattc mtttragttt 20 <210> 3 <211> 20 <212> DNA <213> artificial sequence <220> <223> 27F primer <400> 3 agagtttgat cmtggctcag 20 <210> 4 <211> 22 <212> DNA <213> artificial sequence <220> <223> 1492R primer <400> 4 tacggytacc ttgttacgac tt 22 <210> 5 <211> 1519 <212> DNA <213> unknown <220> <223> Gluconacetobacter intermedius <400> 5 ggggggggtc aattttataa gaggggaatc tttagagttt ggaatcaggc tcagagcgaa 60 cgctggcggc atgcttaaca catgcaagtc gcacgaacct ttcggggtta gtggcggacg 120 ggtgagtaac gcgtagggat ctgtccacgg gtgggggata actttgggaa actgaagcta 180 ataccgcatg acacctgagg gtcaaaggcg caagtcgcct gtggaggaac ctgcgttcga 240 ttagctagtt ggtggggtaa aggcctacca aggcgatgat cgatagctgg tctgagagga 300 tgatcagcca cactgggact gagacacggc ccagactcct acgggaggca gcagtgggga 360 atattggaca atgggcgcaa gcctgatcca gcaatgccgc gtgtgtgaag aaggttttcg 420 gattgtaaag cactttcagc ggggacgatg atgacggtac ccgcagaaga agccccggct 480 aacttcgtgc cagcagccgc ggtaatacga agggggcaag cgttgctcgg aatgactggg 540 cgtaaagggc gcgtaggcgg ttgacacagt cagatgtgaa attcctgggc ttaacctggg 600 ggctgcattt gatacgtggc gactagagtg tgagagaggg ttgtggaatt cccagtgtag 660 aggtgaaatt cgtagatatt gggaagaaca ccggtggcga aggcggcaac ctggctcatg 720 actgacgctg aggcgcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac 780 gctgtaaacg atgtgtgctg gatgttgggt gactttgtca ttcagtgtcg tagttaacgc 840 gataagcaca ccgcctgggg agtacggccg caaggttgaa actcaaagga attgacgggg 900 gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgcagaa ccttaccagg 960 gcttgacatg cggaggccgt gtccagagat gggcatttct cgcaagagac ctccagcaca 1020 ggtgctgcat ggctgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag 1080 cgcaaccctc gcctttagtt gccagcacgt ctgggtgggc actctaaagg aactgccggt 1140 gacaagccgg aggaaggtgg ggatgacgtc aagtcctcat ggcccttatg tcctgggcta 1200 cacacgtgct acaatggcgg tgacagtggg aagccaggtg gtgacaccga gccgatctca 1260 aaaagccgtc tcagttcgga ttgcactctg caactcgagt gcatgaaggt ggaatcgcta 1320 gtaatcgcgg atcagcatgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt 1380 cacaccatgg gagttggttt gaccttaagc cggtgagcga accgcaagga cgcagccgac 1440 cacggtcggg tcagcgactg gggtgaagtc gtacagggta aaacccgaaa aactgtggcc 1500 tcccgcatct atcatcaca 1519 <210> 6 <211> 1383 <212> DNA <213> unknown <220> <223> Gluconacetobacter europaeus <400> 6 catgcttaac acatgcaagt cgcacgaacc tttcggggtt agtggcggac gggtgagtaa 60 cgcgtaggga tctgtccatg ggtgggggat aactttggga aactgaagct aataccgcat 120 gacacctgag ggtcaaaggc gcaagtcgcc tgtggaggaa cctgcgttcg attagctagt 180 tggtggggta aaggcctacc aaggcgatga tcgatagctg gtctgagagg atgatcagcc 240 acactgggac tgagacacgg cccagactcc tacgggaggc agcagtgggg aatattggac 300 aatgggcgca agcctgatcc agcaatgccg cgtgtgtgaa gaaggttttc ggattgtaaa 360 gcactttcag cggggacgat gatgacggta cccgcagaag aagccccggc taacttcgtg 420 ccagcagccg cggtaatacg aagggggcaa gcgttgctcg gaatgactgg gcgtaaaggg 480 cgcgtaggcg gttgacacag tcagatgtga aattcctggg cttaacctgg gggctgcatt 540 tgatacgtgg cgactagagt gtgagagagg gttgtggaat tcccagtgta gaggtgaaat 600 tcgtagatat tgggaagaac accggtggcg aaggcggcaa cctggctcat gactgacgct 660 gaggcgcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgctgtaaac 720 gatgtgtgct ggatgttggg tgactttgtc attcagtgtc gtagttaacg cgataagcac 780 accgcctggg gagtacggcc gcaaggttga aactcaaagg aattgacggg ggcccgcaca 840 agcggtggag catgtggttt aattcgaagc aacgcgcaga accttaccag ggcttgacat 900 gcggaggccg tgtccagaga tgggcatttc tcgcaagaga cctccagcac aggtgctgca 960 tggctgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct 1020 cgcctttagt tgccatcacg tttgggtggg cactctaaag gaactgccgg tgacaagccg 1080 gaggaaggtg gggatgacgt caagtcctca tggcccttat gtcctgggct acacacgtgc 1140 tacaatggcg gtgacagtgg gaagccaggt agcgataccg agccgatctc ataaagccgt 1200 ctcagttcgg attgcactct gcaactcgag tgcatgaagg tggaatcgct agtaatcgcg 1260 gatcagcatg ccgcggtgaa tacgttcgcg ggccttgtac acaccgcccg tcacaccatg 1320 ggagttggtt tgaccttaag ccggtgagcg aaccgcaagg acgcagccga ccacggtcgg 1380 gtc 1383

Claims (17)

Komagataeibacter intermedius NY-Gah 150-2 strain deposited under accession number KCTC 14296BP with excellent ability to produce biogel with high tensile strength. delete A biogel production composition comprising a Komagataeibacter intermedius NY-Gah 150-2 strain deposited under accession number KCTC 14296BP, which has excellent ability to produce biogel with high tensile strength. delete A biogel production method comprising a culturing step of culturing a Komagataeibacter intermedius NY-Gah 150-2 strain deposited under accession number KCTC 14296BP, which has excellent ability to produce biogel with high tensile strength. delete delete delete delete delete delete delete delete delete delete delete delete

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