KR102533978B1 - Method for preparing a composition for controlling weeds using Streptomyces sp. DS001 - Google Patents

Abstract

The present invention relates to a method for manufacturing a composition for controlling weeds using a Streptomyces sp. DS001 strain. By using the same, it is possible to manufacture the composition for controlling weeds with excellent control activity against flowers and weeds, especially Digitaria ciliaris.

Description

Method for preparing a composition for controlling weeds using Streptomyces DS001 strain {Method for preparing a composition for controlling weeds using Streptomyces sp. DS001}

The present invention relates to a method for preparing a composition for controlling weeds using a DS001 strain of the genus Streptomyces.

Weeds or weeds are not absolute, but relative concepts, and they are plants that are not appropriate for the time and place, which occurred when humans started agricultural life. Weeds are major control targets that greatly reduce the productivity and marketability of crops due to competition for light, nutrients, and moisture in crop cultivation, mediate pests, and make agricultural work difficult. Weeds occur throughout the year regardless of location, season, environment, etc., and a lot of effort and money are required to control them. In addition, since there are many types of weeds and they are diverse, they are divided into flower and weeds, broad-leaved weeds, and weeds according to plant taxonomic classification and morphological classification, and divided into annual weeds, monthly weeds and perennial weeds according to life type. Therefore, in general, weeds are classified into flowering plants, annual weeds, perennial weeds, broad-leaved annual weeds, perennial weeds, and panicle annual weeds, perennial weeds, etc., or conversely, annual flowers and weeds, broad-leaved weeds, panicle weeds, or perennial flowers and weeds, broad-leaved weeds, and fireflies weeds. separated by

The stems of grasses and weeds have well-distinguished nodes and internodes, and leaves grow alternately from the nodes, and the leaves are divided into leaf sheaths and leaf blades that surround and protect the stems. The leaf body is narrow and long, and the leaf veins are formed in parallel. Blood, indica, foxtail, foxtail, reed, and silver grass belong to this category. Weeds belonging to the Sedges family have similarities with Sedges, but most of them are distinguished by the fact that the cross section of the stem is triangular and there is no ligule or leaf auricle. The leaves are narrow and ridged, with pointed tips, and a small number of small flowers. These include cockroaches, spiny spinners, olbanggae, barnacles, and tadpole gorillas. A broad leaf weed is a plant that does not belong to the herbaceous weeds or weeds of the family Dipperaceae, and is literally a weed with relatively wide leaves. The leaves are mainly elliptical, ovate, and lanceolate, and the leaf veins are intertwined like a net. Many weeds such as egret, shamrock, mugwort, shepherd's purse, amaranth, waterweed, sputum, and viburnum, which are common around us, belong to this category.

Currently, various organic synthetic herbicides exhibiting high herbicidal activity at low cost are used for weed control, but the emergence of resistant weeds, environmental harm and residual toxicity are pointed out as problems. In particular, the emergence of resistant weeds due to the long-term use of synthetic organic herbicides is recognized as a serious socio-economic problem (Riches et al. 1996). However, after the development of 4-hydroxyphenylpyruvate dioxygenase (DPPH) inhibitors such as benzobicyclon, mesotrione, and tefuryltrione, which exhibit excellent control effects, the development of herbicides with new points of action has begun. There is no current situation (Prisbylla et al. 1993; Schultz et al. 1993). Therefore, although the development of synthetic herbicides with a new structure and a new point of action is required, although the impact of synthetic herbicides on the environment or ecosystem is not serious, a climate that emphasizes income improvement and quality of life is created, making it eco-friendly. Due to growing interest in agriculture and strong regulations in each country to reduce the use of chemical pesticides, the reality is that the conditions for the development of organic synthetic herbicides are not easy.

Indica ( Digitaria ciliaris ) is an annual grass and is a representative field weed that occurs in fields, field embankments, orchards, roadsides and empty areas, and is one of the most problematic weeds in summer field crop cultivation and is an annual flower and weed. Crawling on the ground, new roots come out from the node at the bottom of the stem and spread very quickly. The leaves growing under the stem are 8-20 cm long and 5-15 mm wide and have hairs. The length of the inflorescence is about 4-8 mm, very thin and straight, and the stem splits into 3-8 branches, and the inflorescence is reddish or purple with thin ears. It germinates a lot when the soil is dry or wet conditions alternate, and the optimum germination condition is formed especially in spring at a soil temperature of 12~15℃. When it invades farmland, there is a problem of inhibiting the growth of crops by competing with crops mainly for nutrients and space.

Accordingly, the present inventors, while trying to find an eco-friendly natural product-derived herbicide as a new concept herbicide, found that the novel Streptomyces sp. DS001 strain or its culture medium has weed control activity, especially against Digitaria ciliaris. After confirming the herbicidal activity, a method for preparing a weed control composition exhibiting excellent herbicidal activity by culturing this strain was developed.

Republic of Korea Patent Registration No. 10-1785689 (2017.10.17. Notice)

An object of the present invention is to provide a method for preparing a composition for controlling weeds using the Streptomyces genus DS001 strain.

Another object of the present invention is to provide a method for preparing a composition for controlling weeds using a DS001 strain of the genus Streptomyces having herbicidal activity against indica ( Digitaria ciliaris ).

The problem to be solved by the present invention is not limited to the above-mentioned problem (s), and another problem (s) not mentioned will be clearly understood by those skilled in the art from the following description.

In order to solve the above problems, the present invention provides a method for producing a composition for controlling weeds, characterized in that culturing the Streptomyces sp. DS001 strain (KCTC15136BP).

The composition may include Streptomyces sp. DS001 strain (KCTC15136BP) or a culture solution thereof.

The composition may inhibit weed germination and have herbicidal activity.

Method for producing the composition for controlling weeds may be to incubate the strain at a temperature of 25 to 30 ℃.

The manufacturing method of the weed control composition may be to culture the strain at an agitation speed of 150 to 200 rpm.

The manufacturing method of the weed control composition may be to culture the strain at pH 5.5 to 7.

According to the present invention, the weed control composition using the Streptomyces sp. DS001 strain exhibits weed control activity, so by culturing the Streptomyces sp. DS001 strain, flowers and weeds, especially indica ( Digitaria ciliaris ) has the effect of preparing a composition for controlling weeds with excellent control activity.

1 is a graph comparing herbicidal activity against indica growth of 16 strains of the genus Streptomyces according to an embodiment of the present invention.
Figure 2 compares the growth inhibitory effect of the Streptomyces genus strain DS001 culture medium according to an embodiment of the present invention with that of the control group after 7 days of foliar treatment in indica in greenhouse conditions.
Figure 3 shows a phylogenetic tree based on 16S rRNA gene sequence analysis of Streptomyces genus strain DS001 according to an embodiment of the present invention.
Figure 4a is a graph showing the herbicidal activity after 7 days of culturing Streptomyces strain DS001 according to an embodiment of the present invention at various temperatures, Figure 4b is cultured when Streptomyces strain DS001 is cultured at various stirring speeds It is a graph showing herbicidal activity after 7 days, and FIG. 4c is a graph showing herbicidal activity after 7 days of culture when Streptomyces strain DS001 is cultured at various pHs.
Figure 5 compares the chlorophyll content according to the treatment concentration when treating paraquat, glufosinate ammonium and Streptomyces genus strain DS001 culture medium according to an embodiment of the present invention to indica ( Digitaria ciliaris ) it is a graph
Figure 6 compares electrolyte leakage over time when paraquat, glufosinate ammonium and Streptomyces genus strain DS001 culture medium are treated with cucumber ( C. sativus ) according to an embodiment of the present invention. it is a graph

Before describing the present invention in detail, the terms or words used in this specification should not be construed as unconditionally limited in a conventional or dictionary sense, and in order for the inventor of the present invention to explain his/her invention in the best way It should be noted that concepts of various terms may be appropriately defined and used, and furthermore, these terms or words should be interpreted as meanings and concepts corresponding to the technical idea of the present invention.

That is, the terms used in this specification are only used to describe preferred embodiments of the present invention, and are not intended to specifically limit the contents of the present invention, and these terms represent various possibilities of the present invention. It should be noted that it is a defined term.

In addition, in this specification, it should be noted that singular expressions may include plural expressions unless the context clearly indicates otherwise, and similarly, even if they are expressed in plural numbers, they may include singular meanings. do.

Throughout this specification, when a component is described as "including" another component, it does not exclude any other component, but further includes any other component, unless otherwise stated. It can mean you can do it.

In addition, in the following description of the present invention, a detailed description of a configuration that is determined to unnecessarily obscure the subject matter of the present invention, for example, the known technology including the prior art, may be omitted.

Hereinafter, the present invention will be described in more detail.

The present invention provides a method for producing a composition for controlling weeds, characterized by culturing the Streptomyces sp. DS001 strain deposited with Accession No. KCTC15136BP.

The composition is characterized in that it comprises a DS001 strain of the genus Streptomyces [hereinafter referred to as 'DS001 strain' or 'strain DS001'] or a culture solution thereof.

The DS001 strain of the genus Streptomyces according to the present invention was identified through isolation and culture from various forests and non-agricultural lands in 13 locations in Gyeonggi-do, Gangwon-do, Chungcheongbuk-do, Chungcheongnam-do, Gyeongsangbuk-do, Gyeongsangnam-do and Jeollabuk-do.

The DS001 strain was identified as a new strain showing 99% homology with Streptomyces formicae in a 16S rRNA gene homology search, and was classified as a Streptomyces formicae strain.

The present inventors named the novel strain as Streptomyces sp. DS001 ( Streptomyces sp. DS001) strain and deposited it at the Korea Research Institute of Bioscience and Biotechnology (KCTC) on October 17, 2022, and was given accession number KCTC15136BP.

The culture solution is a result of culturing a microbial strain in a medium and may include metabolites of the microorganism. The culture medium may be a liquid medium in which the strain is cultured, but is not limited thereto, and the strain may be cultured in a conventional microbial culture medium. In addition, the culture solution may be a culture solution in which the strain is cultured or a culture filtrate obtained by physically filtering the culture solution, and the culture solution may be undiluted or diluted to treat weeds. As long as the culture broth exhibits herbicidal activity against weeds, it can be treated with weeds through treatment processes such as filtration, concentration, and dilution.

The composition containing the DS001 strain or its culture medium is characterized in that it has weed control activity. In addition, the weed control activity of the composition may inhibit the germination rate of weeds and have herbicidal activity by being toxic to weeds. The weed control activity causes the leaves to turn yellowish brown and stop plant growth leading to death. Referring to FIG. 2, as an example of the present invention, it was confirmed that the growth of indica was reduced to lead to death when the culture solution of the DS001 strain was treated. In addition, referring to Table 1, as an example of the present invention, when soil was treated with a culture solution of strain DS001, effective germination inhibition and herbicidal activity were shown on indica seeds.

The composition may include a diluent containing 25% to 100% of the DS001 strain or a culture thereof, preferably a dilution containing 50% to 100% of the DS001 strain or a culture thereof, more preferably Preferably, it may include a diluted solution containing 75% to 100% of the DS001 strain or its culture medium. Specifically, according to Table 1, the dilution solution containing 25% to 100% of the DS001 strain or its culture medium suppresses the germination rate of weeds to less than 30%, and the dilution solution containing 50% to 100% suppresses the germination rate of weeds to less than 10%. , and the diluted solution containing 75% to 100% has the effect of suppressing the germination rate of weeds to 0%. In addition, the diluted solution containing 25% to 100% of the DS001 strain or its culture medium removes weeds by 30% or more, and the diluted solution containing 50% to 100% suppresses the germination rate of weeds by 40% or more, and contains 75% to 100% One diluted solution has the effect of suppressing the germination rate of weeds by more than 75%.

The composition containing the DS001 strain or its culture may exhibit control activity against flowers and weeds, and in particular, may exhibit weed control activity against indica ( Digitaria ciliaris ), but is not limited thereto.

The composition containing the DS001 strain or its culture medium can inhibit photosynthesis by causing loss of chlorophyll in weeds. Photosynthesis inhibition inhibits light-dependent reactions and electron transport systems in chloroplasts, accumulates active oxygen, oxidizes cell membranes, and causes necrosis, so chlorophyll loss is used as an indicator of herbicidal activity. Figure 5 compares the chlorophyll content according to the treatment concentration when paraquat, glufosinate ammonium, and strain DS001 culture medium were treated with indica. Referring to this, the culture medium of strain DS001 gradually decreased in concentration as the concentration increased, It has been found to inhibit photosynthesis by reducing the chlorophyll content of weeds.

The composition containing the DS001 strain or its culture medium can destroy the cell membrane of weeds. 6 is an analysis of electrolyte leakage after treatment of strain DS001 culture medium, paraquat and glufosinate ammonium in order to determine the destruction of plant cell membranes. It was gradually increased, so it can be seen that the strain DS001 culture medium destroyed the cell membrane of the weed.

The DS001 strain is preferably cultured at a temperature of 20 to 35 ° C., more preferably at a temperature of 25 to 30 ° C. for weed control activity. Referring to Figure 4a, it can be seen that the herbicidal activity of the DS001 strain is the highest at a temperature of 25 to 30 ° C, and the herbicidal activity decreases thereafter.

The DS001 strain can be cultured at an agitation speed of 50 to 200 rpm for weed control activity, preferably cultured at an agitation speed of 100 to 200 rpm, and most preferably cultured at an agitation speed of 150 to 200 rpm. Referring to Figure 4b, it can be seen that the herbicidal activity of the DS001 strain is the highest at an agitation speed of 150 to 200 rpm.

The DS001 strain may be cultured at pH 3.0 to 10, pH 5.5 to 10, preferably cultured at pH 5.5 to 8.5, and most preferably cultured at pH 5.5 to 7.0 for weed control activity. do. Referring to Figure 4c, it can be seen that the herbicidal activity of the DS001 strain is the highest at pH 5.5 to 7.0, and the herbicidal activity decreases thereafter.

Example

Hereinafter, examples will be described in detail to explain the present invention in detail. However, the embodiments according to the present invention can be modified in many different forms, and the scope of the present invention should not be construed as being limited to the embodiments described below. The embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Materials and Methods

1. Soil samples

Soil samples were collected from plant rhizospheres at a depth of 10-20 cm in various forests and non-agricultural lands in 13 locations in Gyeonggi-do, Gangwon-do, Chungcheongbuk-do, Chungcheongnam-do, Gyeongsangbuk-do, Gyeongsangnam-do, and Jeollabuk-do during April 2018, and stored in the laboratory at 4℃. did Each sample was dried at room temperature, ground, and prepared by passing through a 2 mm mesh sieve.

2. Isolation of Streptomyces and Preparation for Culture

1 g of each sample was suspended in 9 mL of distilled water in a sterile test tube and vortexed. The soil solution was then serially diluted to 10 ^-3 , then 100 μL aliquots of the dilutions were added to humic acid-vitamin (HV) agar [20 g bacteriological agar, 0.5 g MgSO ₄ ]. , 5 g Na ₂ HPO ₄ , 0.2 g CaCO ₃ , 10 g humic acid, 20 g KCL, 5 g cycloheximide, and 1 L distilled water). Culture plates were incubated at 25° C. in the dark for 14 days until bacterial colonies sporulated. A representative colony of Streptomyces was selected and smeared on a fresh Bennett's agar plate [Bennet's agar plate: glucose 10 g, yeast extract 1 g, peptone 2 g, beef extract 1 g, microbial agar 20 g, distilled water 1 L] After incubation at 25 ℃ for 7 hours. Pure colonies of Streptomyces strains isolated from Bennett's agar medium were cultured in fresh M3 broth (20 g soytone, 20 g glucose, 40 g soluble starch, 6 g CaCO ₃ and 1 L distilled water) at 27°C for 7 days. were cultured on a rotary shaker (160 rpm). Bacterial biomass was harvested by centrifuging the fermentation broth for 10 minutes (8,000 rpm).

3. Foliage treatment effect of Streptomyces culture medium

The herbicidal activity was confirmed by treating the Streptomyces strain culture medium to Indica ( Digitaria ciliaris ) under greenhouse conditions (30/25 ± 5 ° C., light/dark = 14/10 h). Indica is an important weed in field crops and can cause high yield losses if not controlled. 20-25 Indica seeds were sown in pots containing horticultural medium and grown for 7-10 days in greenhouse conditions. 5 mL of the Streptomyces culture filtrate was sprayed on the leaves of Indica. 0.1% tween-20 was added to all treatments. Herbicidal activity was evaluated at 7 DAT (days after treatment) compared to untreated control plants. Evaluation of physiological and morphological symptoms of mortality was evaluated based on mortality rates of the Brazilian Academy of Weed Sciences (SBCPD) (0% = no damage, 20-40% = slight damage or effect of growth with rapid recovery) or reduction, 40-60% = moderate damage or slow recovery or eventual reduced growth, 60-80% = severe damage or irreversible growth reduction and/or reduced stand, 80-100% = complete destruction or very few survivors).

4. Soil treatment effect of Streptomyces culture medium

Twenty seeds of Indica ( Digitaria ciliaris ) were planted to a depth of 1-2 cm in pots containing commercial horticultural medium. The concentrations of the culture filtrate of Streptomyces strain DS001 were diluted to 25%, 50%, 75% and 100% for soil treatment. Each culture filtrate in a volume of 5 mL per pot was sprayed. Distilled water was used as a control. The experiment was performed in a completely randomized method with three repetitions. Herbicidal activity was evaluated 14 days after treatment. Seed germination rate was calculated by the following equation.

[formula]

Seed germination rate = (number of germinated seeds/total number of seeds) x 100

5. 16S rRNA gene sequence analysis

Streptomyces strains with the highest herbicidal activity were characterized through 16S rRNA gene sequencing. 16S rRNA was amplified using universal primers 27F (5′-AGAGRRTGARCCTGGCTCAG-3′) and 1492R (5′-GGRTACCTTGRRACGACTT-3′). Genomic DNA was obtained from single colonies of bacterial cells disrupted by heating at 100°C for 10 minutes. For sporulation strains, bacterial cells were disrupted in a microwave at 650 W for 30 seconds in buffer. The suspension was centrifuged at 13,000 rpm for 10 minutes. After centrifugation, 2 μL of supernatant was used for PCR. PCR products were purified and sequenced at SolGent Inc. (Daejeon, Korea). The resulting sequences were analyzed with the BLASTn program at NCBI (http://www.ncbi.nlm.nih.gov). The phylogenetic tree was generated by the neighbor-joining method using the MEGA 6.0 program.

6. Setting up culture conditions

To measure the herbicidal activity of Streptomyces strain DS001, bacterial isolates were inoculated into 500 mL Erlenmeyer flasks containing 200 mL M3 broth. After inoculation, flasks with bacterial strains were incubated at temperatures ranging from 160 rpm to 20, 25, 30 and 35 °C, agitation rates ranging from 50, 100, 150 and 200 rpm, and initial pHs of 3, 5.5, 7, 8.5, 10 After culturing with shaking at 27 ℃ for 7 days in a shaker, the culture filtrate was treated and tested. Dalgwan evaluation was conducted 7 days after treatment.

7. Inhibition of photosynthesis

Indica seeds were grown in pots (6 cm Х 9 cm) for 2 weeks under greenhouse conditions (30/25 ± 5 °C, light = 14/10 h). The culture filtrate of Streptomyces strain DS001 at 100% concentration, paraquat (1,155 g a.i./ha) and glufosinate ammonium (900 g a.i./ha) were treated with plants in the 2-3 leaf stage. . After treatment, the plants were cultured in the growth phase (30/25±5° C., light/dark = 12/12 hours). Photosynthesis inhibition rate of the leaves of indica was measured 24 hours after treatment with a chlorophyll fluorescence meter (Handy PEA, Hansatech Instruments, UK). All treatments were repeated 3 times in each test.

8. Electrolyte leakage analysis

One-week-old cucumber ( Cucumis sativus ) cotyledons were used for electrolyte leakage assay. A total of 30 leaf discs (approximately 5 mm in diameter) were placed in a Petri dish containing 1 mM of MES buffer, followed by 5 mL of Streptomyces culture filtrate, paraquat (1,155 g ai/ha) and ammonium glufosinate (900 g). ai/ha) were each added to the petri dish. An untreated control was prepared in the same manner as above except that 5 mL of water was added. After treatment, the petri dish was placed on a growth bed at 25°C for culture. Electrolyte leakage was measured at 0, 2, 4, 6, 8, 10 and 24 HAT using an electronic conductivity meter (TOA-DKKCM-21P, DKK TOA, Japan).

result

1. Foliage treatment effect of Streptomyces strains with herbicidal activity

As a result of the screening, 16 strains (4.8%) out of 332 strains showed significant inhibitory activity against indica growth. The sources of the 16 strains were identified as 4 strains from Gangwon-do, 10 strains from Gyeongsangbuk-do and Chungcheongnam-do, and 2 strains from Gyeongsangnam-do.

1 shows the herbicidal activity of 16 strains selected for the growth of indica. Referring to FIG. 1, among the 16 selected strains, three strains, DS012, DS077, and DS001, showed significant activity against the growth inhibition of Indica. At 7 days after treatment, the effects on indica in the treatment of strains DS012 and DS077 were 88.3% and 89.67%, respectively, while the growth of indica after treatment with strain DS001 was reduced by 95.5%.

Strains DS012 and DS077 showed herbicidal activity but did not completely inhibit the growth of indica. Referring to FIG. 2, strain DS001 turns the leaves of indica yellow, stops the growth of the plant, and eventually leads to death. The strain DS001 was named as Streptomyces sp. DS001 strain and deposited at the Korea Research Institute of Bioscience and Biotechnology (KCTC) on October 17, 2022, and was given accession number KCTC15136BP.

2. Soil treatment effect of Streptomyces strains with herbicidal activity

In the soil treatment experiment of Streptomyces strain DS001, the germination rate of indian seeds was sensitive at all concentrations compared to the untreated control (95±1.25) (25%: 30±2.13, 50%: 10±1.53, 75%: 0, 100%: 0).

Referring to Table 1, in the greenhouse experiment, the Streptomyces strain DS001 culture filtrate was treated at all concentrations (25%: 36.67±5.77, 50%: 45±15.00, 75%: 75±5.00) compared to the untreated control on day 7. , 100%: 90±5.00) showed an effective activity in inhibiting seed germination of Indica.

Culture filtrate concentration of Streptomyces strain DS001 0% 25% 50% 75% 100% Germination rate (%) 95±1.25 30±2.13 10±1.53 0 0 Prevention (%) 0 36.7±5.77 45.0±15.0 75±5.0 90±5.0

3. 16S rRNA gene sequence analysis of Streptomyces strain DS001

The isolated Streptomyces strain DS001 was identified by sequencing the 16S rRNA gene by PCR using universal primers. Phylogenetic analysis of Streptomyces strain DS001 was constructed based on the neighbor-joining method using 16S rRNA sequences and 16S rRNA sequences of 15 species of the genus Streptomyces. The nucleotide sequence data is registered in GenBank under the accession number NR146048.1. Based on the BLAST results, the isolated Streptomyces strain DS001 was 99% similar to Streptomyces formicae (1H-GS9) as shown in FIG. 3 .

4. Influence of temperature, agitation and pH on the growth of Streptomyces

Streptomyces strain DS001 was cultured at the aforementioned temperatures (20, 25, 30 and 35° C.), agitation rates (50, 100, 150 and 200 rpm) and pH (3.0, 5.5, 7.0, 8.5 and 10.0). The results are shown in Figures 4a, 4b and 4c.

Referring to Figures 4a, 4b and 4c, while the Streptomyces strain showed high herbicidal activity at a temperature of 25-30 ° C, an agitation speed of 150-200 rpm, and a pH of 5.5-7, at higher temperatures or higher Herbicidal activity was shown to decrease at higher pH.

5. Effect on chlorophyll content

Herbicides that inhibit photosynthesis inhibit light-dependent reactions and electron transport systems in chloroplasts, resulting in necrosis by accumulating reactive oxygen species and oxidizing cell membranes. Thus, a decrease in chlorophyll is used as an indicator of herbicidal activity for active herbicidal metabolites. The chlorophyll content of the indica treated with Streptomyces strain DS001, paraquat, and glufosinate ammonium was evaluated using a chlorophyll fluorometer, and the results are shown in FIG. 5 .

Referring to Figure 5, it was observed that the chlorophyll content generally decreased as the treatment concentration increased. In the case of paraquat, the chlorophyll content decreased rapidly as the concentration increased. However, Streptomyces strain DS001 and glufosinate ammonium showed a slight deviation compared to paraquat. Indica treated with paraquat (100%) showed a 96% reduction in chlorophyll content compared to the untreated control. On the other hand, it was observed that the chlorophyll content decreased by 65% and 61% compared to the untreated control when the culture filtrate of Streptomyces strain DS001 and 100% concentration of glufosinate ammonium were treated. Therefore, it was confirmed that the culture filtrate of Streptomyces strain DS001 inhibited photosynthesis.

6. Electrolyte leakage

Electrolyte leakage analysis was performed after treatment with Streptomyces strain DS001, paraquat and glufosinate ammonium in order to examine the destruction of plant cell membranes, and the results are shown in FIG. 6 .

Referring to Figure 6, while the electrolyte leakage rapidly increased between 2 hours and 24 hours after paraquat treatment, it showed a rapid increase between 4 hours and 10 hours after Streptomyces strain DS001 culture filtrate and glufosinate ammonium treatment. The amount of electrolyte leakage caused by Streptomyces strain DS001 was not as rapid as that caused by paraquat, but was higher than that of glufosinate ammonium. After 24 hours of treatment with the three herbicides, large amounts of electrolyte leakage were confirmed in the order of paraquat, Streptomyces strain DS001 culture filtrate, and ammonium glufosinate. The Streptomyces strain DS001 culture filtrate showed a gradual increase in electrolyte leakage over time up to 24 hours after treatment. Therefore, it can be seen that the plant cell membrane was destroyed over time after the treatment of the Streptomyces strain DS001 culture filtrate.

So far, specific examples of the method for preparing a composition for controlling weeds using the Streptomyces genus DS001 strain of the present invention have been described, but it is apparent that various modifications are possible within the limits that do not deviate from the scope of the present invention.

Therefore, the scope of the present invention should not be limited to the described embodiments and should not be defined, and should be defined by not only the claims to be described later, but also those equivalent to these claims.

That is, it should be understood that the above-described embodiments are illustrative in all respects and not restrictive, and the scope of the present invention is indicated by the claims to be described later rather than the detailed description, and the meaning and scope of the claims and All changes or modified forms derived from the equivalent concept should be construed as being included in the scope of the present invention.

Korea Research Institute of Bioscience and Biotechnology Biological Resources Center (KCTC) KCTC15136BP 20221017

Claims (6)

In the method for producing a composition for controlling weeds for culturing Streptomyces formicae DS001 strain (KCTC15136BP),
The composition comprises the culture solution of the Streptomyces formicae DS001 strain (KCTC15136BP) in 50 to 100% of the total composition,
The composition inhibits the germination rate of indica ( Digitaria ciliaris ) by soil treatment and has herbicidal activity,
Characterized in that the strain is cultured in M3 liquid medium,
Method for producing a composition for controlling weeds.
delete delete According to claim 1,
Characterized in that the strain is cultured at a temperature of 25 to 30 ° C.
Method for producing a composition for controlling weeds.
According to claim 1,
Characterized in that the strain is cultured at an agitation speed of 150 to 200 rpm,
Method for producing a composition for controlling weeds.
According to claim 1,
Characterized in that the strain is cultured at pH 5.5 to 7,
Method for producing a composition for controlling weeds.

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