KR102010471B1 - Streptomyces drozdowiczii KRA16-334 strain and use thereof - Google Patents

Abstract

The present invention relates to a Streptomyces drozdowiczii KRA16-334 strain deposited with accession number KCTC 13357BP and uses thereof. The Streptomyces drozdowiczii KRA16-334 strain or culture medium thereof of the present invention exhibits herbicidal activity against Solanum nigrum, Aeschynomene indica, Abutilon avicennae, Xanthium strumarium, and Calystegia japonica, which are broadleaf weeds, and Sorghum bicolor, Echinochloa crus-galli, Digitaria sanguinalis, Agropyron smithii, and Panicum dichotomiflorum, which are grassy weeds, and thus can be advantageously used in herbicide compositions for weed control and a weed control method.

Description

Streptomyces drozdowiczii KRA16-334 strain and use approximately}

The present invention relates to a Streptomyces drosedowicki KRA16-334 strain and its use.

Weeds or weeds are not absolute, but relative. They are plants that originate from the beginning of agricultural life and are not appropriate for the time and place. Weeds are divided into plant weeds, weeds, broadleaf weeds, and moths and weeds according to plant taxonomic and morphological classifications. Weeds are divided into annual weeds, monthly weeds and perennial weeds.

Grasses Weeds have a distinct segment between the nodes and the nodes, the leaves are separated from the nodes, and the leaves are divided into leaf sheaths and leaf blades that surround and protect the stems. Narrow and elongated leaf vein (leaf vein) is characterized in that formed in parallel. Blood, winter, grass, dog grass, reeds, silver grass, etc. belong to this. Weeds belonging to the sedges have similarities to the flower, but most are distinguished by the fact that the stem cross section is triangular and there are no leaf tongues or leaf ears. The leaves are narrow, ridged, pointed at the end, with a small number of small flowers. Bangdongsani, beebangbangsani, olbang, barberry, tadpoles, etc. belong. Broad leaves Weeds are plants that do not belong to flower beds, weeds, or larvae and weeds, and are literally broad-leaved weeds. The leaves are mainly oval, ovoid and lanceolate, with leaf veins intertwined like a net. Many of the weeds in this category are common, such as forget-me-not, shamrock, mugwort, horseradish, amaranth, stilt, sputum, and ferns.

Weeds are the main targets for the control of crops, nutrients, moisture, etc., which deteriorate productivity and commerciality, or make pests difficult and agricultural work difficult. Weeds occur throughout the year regardless of location, season, environment, etc., and they require a lot of effort and expense to control them. At present, various organic synthetic herbicides having high herbicidal activity at low cost are used for weed control, but the emergence of resistant weeds, environmental hazards and residual toxicity has been pointed out as problems. In particular, the emergence of resistant weeds due to the long-term use of organic herbicides has been recognized as a serious socioeconomic problem (Riches et al. 1996). However, since the development of 4-hydroxyphenylpyruvate dioxygenase (DPPH) inhibitors such as benzobicyclon, mesotrione and tefuryltrione, which have excellent control effects, the development of herbicides having a new action point has been developed. (Prisbylla et al. 1993; Schultz et al. 1993). Therefore, the development of synthetic herbicides with new action points with a new structure is required, but due to the strong interest of eco-friendly agriculture and the strong regulation of each country to reduce the use of chemical pesticides with the development of a climate that emphasizes income improvement and quality of life. The conditions for developing organic herbicides are not green.

It is an object of the present invention to provide a novel Streptomyces drozdowiczii KRA16-334 strain.

Another object of the present invention is to provide a weed control composition comprising Streptomyces drozdowiczii KRA16-334 strain or a culture thereof as an active ingredient, and a method for weed control using the composition.

In order to achieve the above object, the present invention is also a Streptomyces deurojeu deposited as accession number of KCTC 13357BP wick Che (Streptomyces drozdowiczii ) provides a KRA16-334 strain.

The present invention also provides a herbicide composition for weed control comprising Streptomyces drozdowiczii KRA16-334 strain or its culture solution deposited with accession number KCTC 13357BP as an active ingredient.

The present invention also provides a method for controlling weeds, comprising the step of treating Streptomyces drozdowiczii KRA16-334 strain or its culture deposited with weed number KCTC 13357BP to weeds, seeds or habitats thereof. do.

Streptomyces of the present invention ( Streptomyces) drozdowiczii ) KRA16-334 strain or its cultures are herbicide compositions for weed control because they exhibit herbicidal activity in broad-leaf weeds, nymphs, larvae, larvae, hawks and buckwheat and weeds, sorghum, snails, barley, wheat and US cultivars. It can be usefully used for weed control methods.

1 is Streptomyces ( Dreptomyces) drozdowiczii ) Treated culture filtrate of the KRA16-334 strain (supernatant from which the cells were removed from the culture solution) and 5 days after the treatment
(Left: 5 days after treatment with Streptomyces drosedowicki KRA16-334 strain culture filtrate;
Right: before treatment with Streptomyces drosedowicki KRA16-334 strain culture filtrate).
Figure 2 Streptomyces ( Dreptomyces) drozdowiczii ) Phylogenetic tree results based on 16S rRNA sequence information of KRA16-334 strain.
Figure 3 Streptomyces ( Dreptomyces) drozdowiczii ) 5 days after treatment with the stock solution of the culture filtrate of KRA16-334 strain, the 2-fold dilution of the culture filtrate, and the 4-fold dilution of the culture filtrate, respectively, five broad-leaf weeds (cranium, larvae, larvae, skeletal and buckthorn), flowers and weeds were treated. This diagram shows the appearance of five species (sorghum, dolpi, barren, wheat, and American total).

Hereinafter, the present invention will be described in detail.

The present invention provides Streptomyces drozdowiczii KRA16-334 strain deposited with accession number KCTC 13357BP.

Streptomyces drozdowiczii KRA16-334 strain (hereinafter referred to as KRA16-334 strain) according to the present invention was identified through separation culture from the forest soil of Pungsan-ri, Boryeong-gun, Chungcheongnam-do.

The KR16-334 strain was identified as a novel strain showing 99.9% homology with Streptomyces drozdock chy in 16S rRNA gene homology search, which is a Streptomyces drozdowiczii strain. Classified as

The inventors named the novel strain Streptomyces drozdowiczii KRA16-334 strain and deposited it on September 29, 2017 at the Korea Research Institute of Bioscience and Biotechnology (KCTC) with accession number KCTC 13357BP. Was granted.

The present invention also provides a herbicide composition for weed control comprising Streptomyces drozdowiczii KRA16-334 strain or its culture solution deposited with accession number KCTC 13357BP as an active ingredient.

The weeds may be flower beds and weeds or broadleaf weeds, but are not limited thereto. The flower and weeds are Sorghum bicolor , Echinochloa crus - galli ) and dianthus sanguinalis ), wheatgrass ( Agropyron smithii ), and American open field ( Panicum) dichotomiflorum ) may be any one selected from the group consisting of, but is not limited thereto. The broadleaf weed is Solanum nigrum), Aeschynomene indica (Aeschynomene indica), Abutilon theophrasti (Abutilon avicennae), Xanthium strumarium (Xanthium strumarium ) and calystegia japonica may be any one selected from the group consisting of, but is not limited thereto.

The culture solution may be a GSS liquid medium in which the strain is cultured, but is not limited thereto, and may be a conventional microbial culture medium. In addition, the culture solution may be a culture solution cultured strain or a culture filtrate physically filtered the culture solution, the culture solution may be diluted or treated to weeds. The culture solution may be treated to the weeds through treatments such as filtration, concentration, dilution, as long as they exhibit herbicidal activity to the weeds.

The herbicide composition for weed control may include a carrier and / or a diluent. As used herein, the term 'carrier' refers to an inert, organic or inorganic, having the active ingredient mixed or made to facilitate its application, or storage, transport and / or handling thereof, to a plant or other subject to be treated. Mean material. Examples of such diluents or carriers include, but are not limited to, water, milk, ethanol, mineral oil, glycerol.

In a specific embodiment of the present invention, the inventors have isolated a novel Streptomyces drozdowiczii KRA16-334 strain from forest soil samples (see FIG. 2), and said Streptomyces drozdowic tooth KRA16. The culture filtrate of the -334 strain showed herbicidal activity against five broadleaf weeds (Crownia, Albizia julibrissin, estuary, doe and buckwheat), and five species of weeds and sorghum, sorghum, barley, wheat, and US herbicides. See Table 1).

Therefore, the Streptomyces drosedowicki KRA16-334 strain of the present invention or its culture may be usefully used as a herbicide composition for weed control.

The present invention also provides a method for controlling weeds, comprising the step of treating Streptomyces drozdowiczii KRA16-334 strain or its culture deposited with weed number KCTC 13357BP to weeds, seeds or habitats thereof. do.

The treatment may be, but is not limited to, a foliage treatment applied directly to the stems and leaves of the plant, a seed treatment applied to the seeds of the plant, or a soil treatment applied to the soil.

The weeds may be flower beds and weeds or broadleaf weeds, but are not limited thereto. The flower and weeds are Sorghum bicolor , Echinochloa crus - galli ) and dianthus sanguinalis ), wheatgrass ( Agropyron smithii ), and American open field ( Panicum) dichotomiflorum ) may be any one selected from the group consisting of, but is not limited thereto. The broadleaf weed is Solanum nigrum), Aeschynomene indica (Aeschynomene indica), Abutilon theophrasti (Abutilon avicennae), Xanthium strumarium (Xanthium strumarium ) and calystegia japonica may be any one selected from the group consisting of, but is not limited thereto.

In a specific embodiment of the present invention, the inventors of the culture filtrate of Streptomyces drosedowicki KRA16-334 strain 5 broadleaf weeds (Crown, larvae, motherbird, skeletal and buckwheat) and flower and weeds (sorghum, dolpi) , Fructus, wheatgrass, and US herbicide), as a result of foliage treatment, it was confirmed that exhibited herbicidal activity against 10 treated weeds (see Table 1).

Therefore, the Streptomyces drosedowicki KRA16-334 strain or its culture solution of the present invention can be usefully used for weed control methods.

Hereinafter, the present invention will be described in detail by the following examples.

However, the following examples are merely to illustrate the invention, but the content of the present invention is not limited by the following examples.

< Example  1> KRA16 Isolation of -334 strain Passage  culture

Soil samples from the forests of Poongsan-ri, Boryeong-gun, Chungcheongnam-do, Korea (HV acid-vitamin agar) [0.1% humic acid (dissolved in 0.2 N NaOH), 0.05% Na ₂ HPO ₄ , 0.171% KCl, 0.005 % MgSO ₄ , 0.001% FeSO ₄ · 7H ₂ O, 0.002% CaCO ₃ , traces of vitamin B complex, 50 ppm cycloheximide, pH 7.0], isolated KRA16-334 strain, Bennett ) Agar (BA; 1% glucose, 0.1% yeast extract, 0.2% bacto-peptone, 0.1% bee extract, 2% agar, 1 L water) plate Subcultured at.

< Example  2> KRA16 Of -334 strain Culture filtrate  Produce

The KRA16-334 strain isolated in Example 1 was used as an active medium, GSS liquid medium (1% soluble starch, 2% glucose, 2.5% soybean meal, 0.1% bee extract, 0.4% Inoculated with yeast extract (yeast extract), 0.2% NaCl, 0.025% K ₂ HPO ₄ and 0.2% CaCO ₃ ) and incubated for 7 days at 27 ℃, 160 rpm and centrifuged (8,000 rpm, 15 minutes) The cells were removed and concentrated under reduced pressure (EYELA, N-1200A). The culture filtrate was used as a sample for the evaluation of herbicidal activity.

< Example  3> KRA16 -334 strain Of culture filtrate  Evaluation of Barley Herbicide Activity

To evaluate the herbicidal herbicidal activity of the culture filtrate of KRA16-334 strain prepared in Example 2. Specifically, 5 ml of the KRA16-334 strain culture filtrate prepared in Example 2 per spray pot was spray-sprayed and sprayed at 5 ml after 5 days to examine the crown based on the external symptoms (0: no effect; 100: complete test) ).

KRA16-334 strain culture filtrate was subjected to foliage treatment in the bark as a result, began to wither (wilting) symptoms within 24 hours and completely died after 5 days (Fig. 1).

Example 4 Identification and Lineage of KRA16-334 Strains

KRA16-334 strain isolated in Example 1 was identified by 16S rRNA gene sequencing analysis, the analysis was carried out through GenBank database. Among the candidate groups obtained from the base sequence comparison, 20 candidate groups with the highest similarity were sorted using clustal w, and a phylogenetic tree was prepared through the neighbor-joining method (PHYDIT program version 3.0).

16S rRNA gene sequencing of the KRA16-334 strain isolated from Example 1 showed 99.9% homology with Streptomyces drozdowiczii , and a phylogenetic tree was prepared using the 16S rRNA sequence. Results, Streptomyces drozdowiczii ), and the closest (Fig. 2). The KRA16-334 strain of the present invention was named Streptomyces drosedowicki KRA16-334 strain and deposited in the Biotechnology Center of Biotechnology Research Institute (Strain No .: KCTC13357BP).

Example 5 Evaluation of Herbicidal Activity of KRA16-334 Strain from Streptomyces Drosedowick

The herbicidal activity of Streptomyces drosedowicki KRA16-334 strain culture filtrate and dilution against broadleaf weeds and flower and weeds was evaluated.

Specifically, five broad-leaved weeds (Crownwood, Albizia julibrissus, Black-tailed larvae and buckwheat) and five flowers and weeds (sorghum, snails, barley, wheatgrass, and the United States) in a 350 cm ² square plastic pot filled with gardening topsoil. Open field) and seedlings were grown for 12 days in greenhouse conditions (35/25 ± 5 ℃, Light / Dark = 14 / 10h), and then the stock solution of the culture filtrate of the strain Streptomyces drosedowicki KRA16-334 of Example 2. And dilutions (two- and four-fold dilutions) were leaf sprayed at 14 ml per pot, respectively. The herbicidal activity was assessed on the basis of external symptoms 5 days after foliage spray treatment (0; no effect, 100; complete test).

As a result of evaluating the herbicidal activity according to the concentration of the culture filtrate of Streptomyces drozdowicki KRA16-334 strain, 100% herbicidal activity was obtained in the culture filtrate stock and the 2-fold dilutions of four broadleaf weeds except the flower weed and five weeds. In the 4-fold dilution, 100% of the sorghum was 60%, cornflower 80%, Dolphin, barley, American larvae, crows, larvae and larvae (Table 1 and Figure 3).

Weed Herbicide activity (%) Stock solution 2-fold dilution 4-fold dilution Sorghum 100 100 60 Dolphin 100 100 100 wire grass 100 100 100 United States 100 100 100 Under the hood 100 100 100 Silkworm 100 100 100 Bead 40 30 40 Macaw 100 100 100 convolvulus 100 100 80

Korea Research Institute of Bioscience and Biotechnology KCTC13357BP 20170929

Claims (9)

The Streptomyces deurojeu deposited as accession number of KCTC 13357BP also wick Che (Streptomyces drozdowiczii ) KRA16-334 strain.
A herbicide composition for weed control comprising Streptomyces drozdowiczii KRA16-334 strain or its culture solution deposited as accession number KCTC 13357BP as an active ingredient.
The herbicide composition for weed control according to claim 2, wherein the weeds are flower beds and weeds or broadleaf weeds.
The method of claim 3, wherein the flower and weeds are Sorghum bicolor , Echinochloa crus - galli ), dianthus ( Digitaria sanguinalis ), wheat flour ( Agropyron) smithii ), and Panicum dichotomiflorum ) any one selected from the group consisting of, herbicide composition for weed control.
The method according to claim 3, wherein the broadleaf weed is Solanum nigrum), Aeschynomene indica (Aeschynomene indica), Abutilon theophrasti (Abutilon avicennae), Xanthium strumarium (Xanthium strumarium ) and cauliflower ( Calystegia japonica ) is any one selected from the group consisting of, herbicide composition for weed control.
The Streptomyces deurojeu deposited as accession number of KCTC 13357BP also wick Che (Streptomyces drozdowiczii ) Weed control method comprising the step of treating the KRA16-334 strain or its culture in the weeds, seeds or habitat thereof.
The weed control method according to claim 6, wherein the weeds are flower beds and weeds or broadleaf weeds.
The method of claim 7, wherein the flower and weeds are Sorghum bicolor , Echinochloa crus - galli , Digitaria sanguinalis ), wheatgrass ( Agropyron smithii ), and Panicum dichotomiflorum ) any one selected from the group consisting of, weed control method.
The method according to claim 7, wherein the broadleaf weed is Solanum nigrum), Aeschynomene indica (Aeschynomene indica), Abutilon theophrasti (Abutilon avicennae , macaw ( Xanthium) strumarium ) and Caystegia japonica , any one selected from the group consisting of weed control method.

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