KR102520985B1 - Health function food composition comprising lactobacillus for improving skin and vowel function - Google Patents

Abstract

In one aspect of the present invention, a food composition for improving skin and intestinal function, including Lactobacillus sakei, Lactobacillus plantarum, fructooligosaccharide, chicory extract, xylitol and raspberry powder And a manufacturing method thereof is disclosed.
The food composition according to the above embodiment has effects of improving skin and intestinal function.

Description

Health functional food composition containing lactic acid bacteria having skin improvement and intestinal function improvement effects {Health function food composition comprising lactobacillus for improving skin and vowel function}

The following examples relate to a health functional food composition containing lactic acid bacteria having skin improvement and intestinal function improvement effects and a manufacturing method thereof.

Synbiotics is a combination of probiotics and prebiotics. Probiotics refers to bacteria that have a beneficial effect on the intestines or foods containing the bacteria, and prebiotics refers to substances derived from foods that are active in proliferating beneficial bacteria in the intestine.

On the other hand, interest in beauty is growing, and the importance of youthful and vital skin is becoming more prominent. An effective way to have good skin is to prevent skin aging. The main causes of skin aging include skin damage caused by ultraviolet rays and dryness. In order to prevent the skin from drying out, use a moisturizer such as lotion to prevent moisture leakage to the outside as much as possible. In addition, skin regeneration products such as ampoules are used to regenerate damaged skin from ultraviolet rays.

Among them, one that has recently come to mind is improving the condition of the skin by consuming food.

Based on long clinical experience, the present inventors tried to invent a food composition that improves skin conditions and improves intestinal function at the same time.

Korean Patent Application No. 10-2021-0063728

In view of the above problems, the inventors of the present invention include probiotics, prebiotics and raspberry powder, wherein the probiotics include Lactobacillus sakei and Lactobacillus plantarum, The prebiotics are intended to provide a food composition for skin improvement and intestinal function improvement containing fructooligosaccharide, chicory extract and xylitol.

Accordingly, one object of the present invention is to provide a food composition having effects of improving skin and intestinal function by including probiotics, prebiotics and raspberry powder.

In addition, one object of the present invention is to provide a method for producing a food composition having effects of improving skin and intestinal function by including probiotics, prebiotics and raspberry powder.

The object of the present invention is not limited to the object mentioned above. The objects of the present invention will become more apparent from the description that follows, and will be realized by means and combinations thereof set forth in the claims.

A food composition for improving skin and intestinal function according to an embodiment includes probiotics, prebiotics, and raspberry powder, wherein the probiotics include Lactobacillus sakei and Lactobacillus plantarum. and the prebiotics include fructooligosaccharide, chicory extract and xylitol.

The food composition according to an embodiment includes 10 to 20 parts by weight of the Lactobacillus sake, 5 to 10 parts by weight of the Lactobacillus plantarum, 40 to 50 parts by weight of the fructooligosaccharide, and 10 to 20 parts by weight of the chicory extract. , 10 to 20 parts by weight of the xylitol and 5 to 20 parts by weight of the raspberry powder.

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The food composition according to one embodiment further comprises skim milk powder, fish collagen, vitamin C, L-proline, hyaluronic acid, and vitamin A powder, 1 to 10 parts by weight of skim milk powder, and 1 to 10 parts by weight of fish collagen. , 0.1 to 5 parts by weight of vitamin C, 0.01 to 5 parts by weight of L-proline, 0.01 to 5 parts by weight of hyaluronic acid, and 0.00001 to 1 part by weight of vitamin A powder.

The food composition according to one embodiment further comprises skim milk powder, fish collagen, vitamin C, L-proline, hyaluronic acid, and vitamin A powder, and 10 parts by weight of Lactobacillus sakei, Lactobacillus pla 4.9 parts by weight of Lactobacillus plantarum, 45 parts by weight of fructooligosaccharide, 15 parts by weight of chicory extract, 11 parts by weight of xylitol, 8 parts by weight of raspberry powder, 1 to 10 parts by weight of skim milk powder, 1 to 10 parts by weight of fish collagen, It contains 0.1 to 5 parts by weight of vitamin C, 0.01 to 5 parts by weight of L-proline, 0.01 to 5 parts by weight of hyaluronic acid, and 0.00001 to 1 part by weight of vitamin A powder.

The food composition of the embodiments has effects of improving skin and intestinal function.

Specifically, it has a skin immunity enhancing effect and has a dual function of improving intestinal health function.

The effects of the present invention are not limited to the effects mentioned above. It should be understood that the effects of the present invention include all effects that can be inferred from the following description.

Hereinafter, embodiments will be described in detail with reference to the accompanying drawings. However, since various changes can be made to the embodiments, the scope of the patent application is not limited or limited by these embodiments. It should be understood that all changes, equivalents or substitutes to the embodiments are included within the scope of rights.

Specific structural or functional descriptions of the embodiments are disclosed for illustrative purposes only, and may be modified and implemented in various forms. Therefore, the embodiments are not limited to the specific disclosed form, and the scope of the present specification includes changes, equivalents, or substitutes included in the technical spirit.

Although terms such as first or second may be used to describe various components, such terms should only be construed for the purpose of distinguishing one component from another. For example, a first element may be termed a second element, and similarly, a second element may be termed a first element.

It should be understood that when an element is referred to as being “connected” to another element, it may be directly connected or connected to the other element, but other elements may exist in the middle.

Terms used in the examples are used only for descriptive purposes and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, terms such as "include" or "have" are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the art to which the embodiment belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, they should not be interpreted in an ideal or excessively formal meaning. don't

Advantages and features of the present invention, and methods of achieving them, will become clear with reference to the detailed description of the following embodiments taken in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various different forms, only these embodiments make the disclosure of the present invention complete, and common knowledge in the art to which the present invention belongs. It is provided to fully inform the holder of the scope of the invention, and the present invention is only defined by the scope of the claims.

In the embodiments of the present invention, unless otherwise defined, all terms used herein, including technical or scientific terms, are the same as those commonly understood by one of ordinary skill in the art to which the present invention belongs. It has meaning. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the embodiments of the present invention, an ideal or excessively formal meaning not be interpreted as

The shapes, sizes, ratios, angles, numbers, etc. disclosed in the drawings for explaining the embodiments of the present invention are illustrative, so the present invention is not limited to the details shown. In addition, in describing the present invention, if it is determined that a detailed description of related known technologies may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted. When 'includes', 'has', 'consists of', etc. mentioned in this specification is used, other parts may be added unless 'only' is used. In the case where a component is expressed in the singular, the case including the plural is included unless otherwise explicitly stated.

In interpreting the components, even if there is no separate explicit description, it is interpreted as including the error range.

The size and thickness of each component shown in the drawings are shown for convenience of description, and the present invention is not necessarily limited to the size and thickness of the illustrated components.

Each feature of the various embodiments of the present invention can be partially or entirely combined or combined with each other, and as those skilled in the art can fully understand, various interlocking and driving operations are possible, and each embodiment can be implemented independently of each other. It may be possible to implement together in an association relationship.

Unless otherwise specified, all numbers, values and/or expressions expressing components, reaction conditions, or amounts of components used herein are intended to indicate that such numbers, among other things, are inherently different from the measurement taken to obtain such values. Since these are approximations that reflect uncertainty, they should be understood as being qualified by the term "about" in all cases. Also, when numerical ranges are disclosed herein, such ranges are contiguous and include all values from the minimum value of such range to the maximum value inclusive, unless otherwise indicated. Furthermore, where such ranges refer to integers, all integers from the minimum value to the maximum value inclusive are included unless otherwise indicated.

In this specification, where ranges are stated for a variable, it will be understood that the variable includes all values within the stated range inclusive of the stated endpoints of the range. For example, a range of "5 to 10" includes values of 5, 6, 7, 8, 9, and 10, as well as any subrange of 6 to 10, 7 to 10, 6 to 9, 7 to 9, and the like. inclusive, as well as any value between integers that fall within the scope of the stated range, such as 5.5, 6.5, 7.5, 5.5 to 8.5 and 6.5 to 9, and the like. Also, for example, the range of "10% to 30%" includes values such as 10%, 11%, 12%, 13%, etc., and all integers up to and including 30%, as well as values from 10% to 15%, 12% to 12%, etc. It will be understood to include any sub-range, such as 18%, 20% to 30%, and the like, as well as any value between reasonable integers within the scope of the stated range, such as 10.5%, 15.5%, 25.5%, and the like.

In the present specification, "skin" means a tissue covering the body surface of an animal, and is a concept in the broadest sense that includes not only the tissue covering the body surface such as the face or body, but also the scalp and hair.

As used herein, the term "improvement" refers to any activity that at least reduces a parameter associated with alleviation or treatment of a condition, for example, the severity of a symptom.

Hereinafter, the present invention will be described in detail.

One aspect of the present invention includes probiotics, prebiotics and raspberry powder, wherein the probiotics include Lactobacillus sakei and Lactobacillus plantarum, and the prebiotics are It provides a food composition for skin improvement and intestinal function improvement, including lacto-oligosaccharide, chicory extract and xylitol, and a method for preparing the same.

In the present invention, the “probiotics” refers to bacteria that have a beneficial effect on the intestines or foods containing the bacteria. Probiotics refers to live microorganisms or cultures thereof that have a beneficial effect on the host by improving the microbial balance in the host's intestines in the gastrointestinal tract of animals, including humans, and further improve the microbial balance in the body or outside the body other than the gastrointestinal tract. It may mean a microorganism or a culture thereof. The term "culture" as used above refers to a material obtained by removing only microorganisms from a microbial culture medium or separated from a microbial culture medium containing microorganisms using an extraction solvent. Methods for removing microorganisms from the microbial culture medium include centrifugation, organic solvent treatment, and the like. In order to separate and extract useful active components, cells may be treated with ultrasonic waves or concentrated after treatment with a solvent such as methanol acetone.

In the present invention, the “prebiotics” are food-derived ingredients that are active in proliferating beneficial bacteria in the intestine.

In the present invention, the "Lactobacillus sakei" means a type of lactic acid bacteria. Microorganisms of the genus Lactobacillus exist widely in nature and produce lactic acid by using carbohydrates anaerobically. In general, it is known that lactic acid bacteria, such as microorganisms of the genus Lactobacillus, are directly or indirectly added to food to improve the shelf life of food by their metabolite, lactic acid, and to improve the flavor and texture of food. In addition, lactic acid bacteria ingested through fermented foods enter the intestine and then adhere to intestinal epithelial cells, which inhibits and antagonizes pathogenic microorganisms, enhances immune activity, reduces cancer incidence, and reduces carcinogenic enzymes. helps a lot in Therefore, lactic acid bacteria are used as probiotics as well as useful auxiliary means for processing dairy products, meat products, chives, and various salted fish, regardless of East and West. However, in order to use lactic acid bacteria as a probiotic, the lactic acid bacteria must be resistant to gastric acid or bile, so the rate of reaching the intestine must be high, and can adhere to and settle on intestinal epithelial cells or mucous membranes. Preferably, by secreting antibacterial substances to suppress harmful bacteria It should be able to stabilize the intestines, prevent colonization of harmful bacteria, and be usable and safe as food. The microorganism Lactobacillus sakei Probio-65 of the genus Lactobacillus of the present invention has all of the above properties.

In the present invention, the "Lactobacillus plantarum" increases intracellular procollagen production in human skin fibroblasts, restores procollagen expression inhibited by ultraviolet rays, inhibits MMP-1 expression increased by ultraviolet rays, It has excellent skin wrinkle improvement, skin epidermal thickness recovery, MMP-13 expression inhibition, and moisturizing effect in a photodamage animal model using hairless mice, so it has the effect of improving skin wrinkles induced by natural aging or photodamage, so Lactobacillus planta It can be used as a drug, fermented food, beverage, health functional food, cosmetic composition, etc. for the purpose of skin wrinkle improvement and moisturizing effect containing room (Lactobacillus plantarum) as an active ingredient. The Lactobacillus plantarum may be Lactobacillus plantarum HY7714.

In the present invention, "fructooligosaccharide" is an oligosaccharide in which one or more fructose is added in a chain to sugar, and the bonding mode is that the second carbon of the fructose added to the first carbon in the fructose residue part of the sugar is bonded. (β 2 > 1) and it repeats. It is an indigestible sugar that is not hydrolyzed by disaccharide enzymes and has been reported to be used primarily by Bifidobacterium, a beneficial intestinal microorganism of the large intestine, to improve the intestinal flora. Bifidobacteria do not produce harmful metabolites in the intestine, but produce a large amount of organic acids such as lactic acid and acetic acid to inhibit the growth of putrefactive bacteria and pathogenic bacteria sensitive to these acids, and prevent intestinal diseases such as diarrhea by suppressing their colonization in the intestine. It plays a role in increasing resistance to infection by stimulating the immune system (Rao, 1999).

In the present invention, "chicory extract" has the effect of promoting the activity of bifidobacteria in the intestine and the absorption of minerals in the intestine, and has been reported to improve arteriosclerotic cardiovascular disease, anti-obesity, anti-cancer, and diabetes. not clearly identified The chicory extract may be chicory root extract powder. The root extract powder may be prepared by extracting chicory root with hot water to obtain a chicory root extract, freeze-drying the extract, and then pulverizing the extract to 200 mesh or less.

Chicory is an important vegetable in France, Belgium, and the Netherlands. It is estimated to be native to all of Northern Europe, and also grows wild in Kashmir, near Lake Baikal in Siberia, and in the northwestern part of China. In ancient Greece, chicory was cultivated as Seris, and there are many plants similar to chicory called Lachana in Greece today. According to the record of Theophharastos (B.C. 372-286), the ancient Romans cultivated and whitened a wild type of chicory called Intubun and used it as a salad, and according to natural scientist Pliny (A.D. 23-79), ancient Arabian doctors and recorded that the leaf was used.

Chicory is a perennial herb originally belonging to the Asteraceae family, and there are two main varieties. One is a variant that mainly collects roots to make chicory coffee, and its scientific name is Cichorium intybus L. var. It is sativus. And the other is Cichorium intybus L. var. It is a foliosum. Intybin, which has a bitter taste, has the effect of promoting digestion and strengthening the vascular system, and is mainly used in salads or slightly cooked. Salad chicory is used by collecting young leaves growing from the roots in the spring. In 100g of living body, 1% protein, 2% carbohydrates, 23mg calcium, 0.7mg iron, 25mg phosphorus, 10mg vitamins A and C, 1.4mg caffeine, etc. contains this. Recently, it is known to have a great pharmacological effect because it is effective in preventing adult diseases such as diabetes, hypertension, gastroenteritis, and liver disease.

In the present invention, 'extract' refers to separating and obtaining an extract from active ingredient raw materials using an extraction and separation method known in the art, and the extract defined in the present invention is obtained by using an appropriate solvent as described above. It may be extracted by extracting the active ingredient raw material prepared as it is or by grinding. At this time, as an appropriate solvent that can be used to obtain the extract, any solvent acceptable in the art may be used, and water or an organic solvent may be used. For example, extraction solvents such as water, alcohol having 1 to 5 carbon atoms, hexane, chloroform, and butylene glycol may be used alone or in combination, but are not limited thereto.

As an extraction method, methods such as hot water extraction, cold brew extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression may be used. In addition, the desired extract may be additionally subjected to a conventional fractionation process or may be purified using a conventional purification method. There is no limitation on the preparation method of the extract of the present invention, and any known method may be used.

In the present invention, “xylitol” is a pentose alcohol found in various fruits and some vegetables (Washuttle. J. et al., J. Food Sci. 38:1262, 1973), which has a high sugar content and does not require insulin for decomposition. Because it is not stable, it is attracting attention as a sugar substitute for the treatment of diabetes and glucose-6-phosphate dehydrogenase-deficiency diseases (Maekinen. K. K. Adv. Food Res. 25: 137-158, 1979; Ylikahri, R. Adv. Food Res. 25 : 159-180, 1979). In addition, it is used as a carbon source for the cariogenic bacteria Streptococcus mutans and S. sobrinus, and when metabolized, acids that cause caries are not generated, preventing secondary dental caries (anticariogenic) (Maekinen. K. K. Adv. Food Res. 25: 137-158, 1979) It also has the function of extending the storage period of food by inhibiting saprophytes such as Clostridium butyrican and Salmonella typhii ( Emodi, A. Food Thechol. 32: 20-32, 1978). Therefore, it is added to various products such as chewing gum, soft drinks, ice cream and toothpaste.

In the present invention, a commercially available raspberry powder may be used. By adding raspberry powder to whey protein, the flavor of raspberry powder eliminates the milky smell, astringency, and astringent taste of whey protein, and at the same time, the flavor and color of raspberry powder give whey protein food a delicious feeling. In addition, the addition of raspberry powder improves water absorption and makes it easy to mix with liquid or paste-type foods. In addition, the raspberry powder appears to be effective in improving skin and intestinal function, although the mechanism of action is not clearly clarified.

In one aspect of the present invention, a food for improving skin and intestinal function, including the Lactobacillus sakei, Lactobacillus plantarum, fructooligosaccharide, chicory extract, xylitol and raspberry powder composition is provided.

In the present invention, food means a natural product or processed product containing one or more nutrients, and preferably means a product that can be eaten directly through a certain degree of processing, and in a conventional sense, It is intended to include all health functional foods, beverages, food additives and beverage additives.

The food of the present invention includes, for example, various foods, beverages, gum, tea, vitamin complexes, health functional foods, and the like. In addition, in the present invention, the food includes special nutritious food (eg, formula milk, infant, baby food, etc.), processed meat product, fish meat product, tofu, jelly, noodles (eg, ramen, noodles, etc.), health supplement food, seasoning food ( For example, soy sauce, soybean paste, gochujang, mixed paste, etc.), sauces, confectionery (eg, snacks), dairy products (eg, fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various types of kimchi, pickles, etc.), drinks ( Examples include, but are not limited to, fruit, vegetable beverages, soy milk, fermented beverages, etc.), natural seasonings (eg, ramen soup, etc.). can be produced in this way.

In the present invention, the health functional food is a food group or food composition that has added value so that the function of the food acts for a specific purpose by using physical, biochemical, or bioengineering methods, etc. It refers to food designed and processed to sufficiently express body control functions related to recovery. The health functional food may include food additives acceptable in food science, and may further include appropriate carriers, excipients, and diluents commonly used in the manufacture of health functional foods.

In the present invention, a beverage refers to a general term for drinking to quench thirst or enjoy taste, and is intended to include health functional beverages. The beverage is not particularly limited in other ingredients other than including the composition as an active ingredient as an essential ingredient in the indicated ratio, and may contain various flavoring agents or natural carbohydrates as additional ingredients like conventional beverages.

Specifically, it can be confirmed that atopic dermatitis is improved, skin soothing effect, skin trouble relief, and skin moisturizing effect. In addition, it can be confirmed that it is effective for eczema, allergy, etc. and has an effect of enhancing skin immunity.

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In one embodiment, 10 to 20 parts by weight of the Lactobacillus sake, 5 to 10 parts by weight of the Lactobacillus plantarum, 40 to 50 parts by weight of the fructooligosaccharide, 10 to 20 parts by weight of the chicory extract, 10 parts by weight of the xylitol to 20 parts by weight and 5 to 20 parts by weight of the raspberry powder. By satisfying the above range, it is possible to provide a food composition with improved skin and intestinal function. Specifically, it can be confirmed that atopic dermatitis is improved, skin soothing effect, skin trouble relief, and skin moisturizing effect. In addition, it can be confirmed that it is effective for eczema, allergy, etc. and has an effect of enhancing skin immunity.

In one embodiment, the composition further comprises skim milk powder, fish collagen, vitamin C, L-proline, hyaluronic acid, and vitamin A powder, 1 to 10 parts by weight of skim milk powder, 1 to 10 parts by weight of fish collagen , 0.1 to 5 parts by weight of vitamin C, 0.01 to 5 parts by weight of proline, 0.01 to 5 parts by weight of hyaluronic acid, and 0.00001 to 1 part by weight of vitamin A powder. By satisfying the above range, it is possible to provide a food composition with improved skin and intestinal function. Specifically, it can be confirmed that atopic dermatitis is improved, skin soothing effect, skin trouble relief, and skin moisturizing effect. In addition, it can be confirmed that it is effective for eczema, allergy, etc. and has an effect of enhancing skin immunity.

In one embodiment, the composition further comprises skim milk powder, fish collagen, vitamin C, L-proline, hyaluronic acid, and vitamin A powder, and 10 parts by weight of Lactobacillus sakei, Lactobacillus pla 4.9 parts by weight of Lactobacillus plantarum, 45 parts by weight of fructooligosaccharide, 15 parts by weight of chicory extract, 11 parts by weight of xylitol, 8 parts by weight of raspberry powder, 1 to 10 parts by weight of skim milk powder, 1 to 10 parts by weight of fish collagen, 0.1 to 5 parts by weight of vitamin C, 0.01 to 5 parts by weight of L-proline, 0.01 to 5 parts by weight of hyaluronic acid, and 0.00001 to 1 part by weight of vitamin A powder. By satisfying the above range, it is possible to provide a food composition with improved skin and intestinal function. Specifically, it can be confirmed that atopic dermatitis is improved, skin soothing effect, skin trouble relief, and skin moisturizing effect. In addition, it can be confirmed that it is effective for eczema, allergy, etc. and has an effect of enhancing skin immunity.

Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and comparative examples. However, these examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of food composition for skin improvement and intestinal function improvement

The food composition for skin improvement and intestinal function improvement of the present invention was prepared as follows.

Specifically, Lactobacillus sakei probio-65 (accession number: KCTC 10755BP), Lactobacillus plantarum HY7714 (accession number: KCTC 12400BP), fructooligosaccharide, chicory extract, xylitol, raspberry The powder was mixed with the composition shown in Table 1 and aged at 20 to 25 ° C. for 2 to 4 hours.

Examples 2 to 3

As shown in Table 1 below, a food composition was prepared in the same manner as in Example 1, except for changing the composition.

Comparative Examples 1 to 10

As shown in Table 2 below, a food composition was prepared in the same manner as in Example 1, except for changing the composition.

Classification (parts by weight) Example 1 Example 2 Example 3 Lactobacillus sake 10 13 15 Lactobacillus Plantarum 4.9 6 7.5 fructooligosaccharides 45 40 40 chicory extract 10 10 10 xylitol 11 12 10 Raspberry Powder 8 7 8 skim milk powder 3 2.5 2.8 fish collagen 2.5 3 2.7 vitamin C 0.35 0.34 0.33 L-proline 0.1 0.11 0.12 hyaluronic acid 0.1 0.1 0.1 Vitamin A powder 0.011 0.011 0.011

Classification (parts by weight) Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Lactobacillus sake 5 30 4.9 10 10 10 15 13 10 10 Lactobacillus plantarum 2.5 3 10 10 4.9 4.9 7.5 6 4.9 4.9 fructooligosaccharides 40 35 45 40 30 - 40 10 30 - chicory extract 15 8 10 10 5 - 12 5 - 20 xylitol 11 8 8 11 5 - 10 5 - 20 Raspberry Powder 9 8 11 8 25 8 - 8 8 8 skim milk powder 3 2.5 3 2.8 2.5 3 3 2.8 2.8 2.8 fish collagen 3.5 3 3.5 2.7 3 2.5 3.5 2.7 2.7 2.7 vitamin C 0.35 0.34 0.35 0.33 0.34 0.35 0.35 0.33 0.33 0.33 L-proline 0.1 0.11 0.1 0.12 0.11 0.1 0.1 0.12 0.12 0.12 hyaluronic acid 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Vitamin A powder 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011 0.011

Test Example 1: Confirmation of atopic dermatitis improvement effect

Atopic mice (NC/Nga Tnd Mice) and normal control mice were acclimatized while maintaining a temperature of 25° C. and humidity of 60±5% and changing the light/darkness at 12-hour intervals.

Before breeding with the experimental diet, they were adapted to a solid feed for a week, and the experimental diet was supplied to atopic mice for 5 weeks by dividing them according to body weight in an egg mass method. Food compositions according to Example 1 and Comparative Examples 1 to 10 were mixed and supplied corresponding to 5% of the total weight of the diet.

Normal control: mice fed normal diet

Atopic dermatitis group: atopic induced experimental group mice

Example 1 and Comparative Examples 1 to 10: Atopic induced experimental group mice + each Example / Comparative Example diet input

<Measurement of lost skin moisture (TEWL)>

In order to measure the water loss of the epidermis (TEWL: tans epidermal water loss), the back of the mouse was shaved before slaughter, left for 30 minutes in a space where constant temperature and humidity were maintained, and then a probe of a hydrometer was applied to the back of the skin. Table 3 shows the values obtained by lightly pressing in close contact with the surface.

division normal control group atopic dermatitis group Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Moisture loss (g/m2/hr) 5.8 10 5.9 8.7 6.4 7.8 7 6.4 8 7.6 7.5 6.5 6.3

In the results of Table 3, as a result of measuring the water loss of each group, the atopic dermatitis group had the highest water loss, and the normal control group showed the lowest. On the other hand, in the case of Example 1, it was confirmed that the epidermal water loss was similar to that of the normal control group, and the water loss inhibitory effect was superior to that of Comparative Examples 1 to 10.

<Clinical measurement of atopic dermatitis: behavioral analysis of itching>

The frequency of scratching of the whole body, including the face, ears, and legs, was recorded using an automatic video camera for 20 minutes on a weekly basis for 5 animals in each group. The results for this are shown in Table 4.

division normal control group atopic dermatitis group Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Number of scratches (times/10 minutes) 4.1 11 5.6 8.6 6.2 7.7 7.1 6.5 8.1 7.4 7.3 6.2 6.4

From the results of Table 4, in the case of Example 1 of the present invention, the number of scratches was significantly reduced compared to Comparative Examples 1 to 10. This result shows that the case of Example 1 of the present invention improves itching.

Experimental Example 2: Confirmation of skin improvement effect

In the case of continuous administration of the food composition of the present invention, the following experiment was performed to confirm whether the skin could be improved. First, the compositions of Examples 1 to 3 and the compositions of Comparative Examples 1 to 10 were administered to 70 women in their 20s to 40s for 1 month, and the skin improvement effect was confirmed.

Specifically, the 70 people were divided into groups of 5 people each, and the compositions of Examples and Comparative Examples were administered three times a day on an empty stomach. On the other hand, in the case of Example 1, the group (Example 1-1) ingested with meals twice a day was also experimented with.

In order to confirm the skin improvement effect, the degree of skin soothing and skin trouble occurrence was measured and a 10-point scoring method was used to give a higher score as the effect was excellent. This is shown in Table 5.

division Example 1 Example 1-1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 skin soothing effect 9.3 9.0 9.2 9.1 7.5 8.5 6.9 7.3 8.3 7.7 8.0 8.4 8.4 8.0 skin trouble relief 9.6 9.0 9.6 9.5 7.3 8.4 6.8 7.4 8.4 7.6 8.4 8.2 8.3 8.0 moisturizing the skin 9.4 8.9 9.3 9.5 7.0 7.5 7.0 7.2 8.2 7.4 8.5 8.2 8.4 7.9 overall satisfaction 9.4 9.0 9.3 9.4 7.2 7.8 6.9 7.2 8.3 7.5 8.3 8.2 8.4 7.9

As shown in Table 5, when the food compositions of Examples 1 to 3 of the present invention were consumed three times a day on an empty stomach, excellent skin soothing, skin trouble relief, and skin moisturizing effects were confirmed. On the other hand, in the case of Comparative Examples 1 to 10, it was confirmed that the effect was lower than that of Examples. On the other hand, as can be seen in Example 1-1, the best effect can be obtained when ingested three times a day on an empty stomach, and it can be confirmed that the effect is somewhat lowered otherwise.

Experimental Example 3: Confirmation of intestinal function improvement effect

<Irritable Bowel Syndrome Improvement Confirmation>

Irritable bowel syndrome improving function of the food compositions according to Examples and Comparative Examples was evaluated by a restraint stress-induced fecal pellet output model [S. Kobayashi et al., Jpn. J. Pharmcaol., 86, p 281-288, 2001].

Male Sprague-Dawley rats (Charles River) weighing 250 to 300 g were used as experimental animals, and the SD rats were used per cage in an animal room controlled at a temperature of 25 ° C, humidity of 50%, and a day-night cycle of 12:12 hours. Two were bred. Free access to water and feed was allowed, and after 5 days of adaptation, restraint experiments were conducted.

On the day of the experiment, restraint-induced fecal pellet output was measured in SD rats using a restraint cage. To this end, the food compositions of each of Examples and Comparative Examples were dissolved in a 0.5% (w.v) CMC aqueous solution and orally administered at a concentration of 300 mg/kg, and the experimental animals were placed in a correction frame. At this time, care was taken so that the animals did not receive stress due to administration. When unable to move within the correction frame, the animal undergoes confinement stress and begins defecation.

The aspect and number of stools were measured at 60-minute intervals for 4 hours, and the results are shown in Table 6. Statistical processing was tested for significance at the level of p<0.01 (**) or p<0.001 (***) using Student's t-test. This is shown in Table 6.

division stress-free group stress treatment group Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 number of bowel movements 3 12 3 3 3 7 6 8 7 5 7 5 7 6 8 Diarrhea frequency 0 8 0 0 0 8 5 8 7 4 7 4 8 5 7 abdominal condition normal bloating normal normal normal bloating bloating bloating bloating bloating bloating bloating bloating bloating bloating

As shown in Table 6, it was confirmed that the group treated with the food composition of the present invention significantly decreased the number of bowel movements caused by confinement stress, and discharged stool similarly to the untreated group to which no confinement stress was applied. On the other hand, in Comparative Examples 1 to 10, most of the stool conditions were checked as diarrhea, and abdominal distension was also severe.

<Analysis of defecation promotion effect>

In order to evaluate the defecation promoting effect of the food compositions prepared in Examples 1 to 3 and Comparative Examples 1 to 10, a survey was conducted by the following method.

The compositions of Examples 1 to 3 and the compositions of Comparative Examples 1 to 10 were administered to 70 women in their 20s to 40s who usually have symptoms of constipation for 1 month, and the defecation promoting effect was analyzed.

Specifically, the 70 people were divided into groups of 5 people each, and the compositions of Examples and Comparative Examples were administered three times a day on an empty stomach. On the other hand, in the case of Example 1, the group (Example 1-1) ingested with meals twice a day was also tested.

For a total of 30 days, the test period, each sample was provided in 500 ml bottled water to the test subjects, and the drinking time was allowed to be consumed at any time of the day.

Successful defecation and defecation time were measured every day for 7 days of drinking intake. For the amount of stool, 35 g or less 1 point, 35-80 g 2 points, 80-200 g 3 points, 200-300 g 4 points, and 300 g or more 5 points evaluated. In addition, the type of stool was measured as diarrhea 1 point, soft stool 2 points, normal stool 3 points, hard stool 4 points, and small stool 5 points.

Side effects that occur during defecation (sensation of residual stool, strength, gas generation, abdominal distension, bubbling) are not at all 1 point, none 2 points, none 3 points, normal 4 points, present 5 points, severe 6 points, Very severe 7 points were evaluated on a total of 7 points scale, and the results are shown in Table 7.

division Example 1 Example 1-1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Defecation success rate (%) 90.1 86.2 89.2 87.5 79.6 85.6 65.4 72.3 79.8 62.4 78.5 83.2 85.2 86.2 time (minutes) 450.4 470.8 462.3 465.2 550.6 548.3 600.2 560.4 534.6 650.2 460.7 490.2 500.1 512.1 amount of stool 3.1 3.2 3.3 3.4 2.8 3.7 1.8 3.7 2.9 2.7 2.9 3.3 3.4 3.2 shape of stool 2.9 2.8 2.8 2.6 3.4 2.7 4.2 2.5 3.5 4.3 3.8 3.4 3.2 3.3 gassing side effects 2.9 3.3 2.9 2.8 3.6 3.7 4.1 3.5 3.7 3.8 3.9 2.9 3.4 3.2 flatulence side effects 2.4 3.0 2.6 2.8 3.7 3.8 4.3 3.3 3.1 3.4 3.7 3.1 3.1 2.9

As shown in Table 7, in the case of Examples 1 to 3, the defecation success rate was significantly high, whereas in Comparative Examples 1 to 10, the defecation success rate increased, but the increase was lower than that of the Examples.

Even in the defecation time, Examples 1 to 3 showed 400 minutes, and the effect was higher than that of Comparative Examples. Even in the case of the amount and shape of the stool, it was confirmed that the food composition according to the present invention received a score close to 3 and was a normal stool.

When synthesizing the above results, the food composition of the present invention shows a remarkable effect on improving various indicators related to skin improvement and intestinal function, and it can be confirmed that it can be used as a food composition having an effect of improving skin and intestinal function. could

Although the embodiments of the present invention have been described with reference to the accompanying drawings, those skilled in the art may implement the present invention in other specific forms without changing its technical spirit or essential features. You will understand that there is Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (3)

Lactobacillus sakei, Lactobacillus plantarum, fructooligosaccharide, chicory extract, xylitol, raspberry powder, skim milk powder, fish collagen, vitamin C, L-proline, hyaluronic acid and vitamin A powder As a food composition for skin improvement and intestinal function improvement comprising a,
10 parts by weight of the Lactobacillus sake, 4.9 parts by weight of the Lactobacillus plantarum, 45 parts by weight of the fructooligosaccharide, 10 parts by weight of the chicory extract, 11 parts by weight of the xylitol, 8 parts by weight of the raspberry powder, the skim milk powder 3 parts by weight, 2.5 parts by weight of the fish collagen, 0.35 parts by weight of the vitamin C, 0.1 parts by weight of the L-proline, 0.1 parts by weight of the hyaluronic acid, and 0.011 parts by weight of the vitamin A powder. Food composition for improving function.

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