KR102506958B1 - Composition Comprising Mesembryanthemum crystalinum Extracts, Pueraria lobata flower Extracts, and Artemisia indica Extracts for Preventing of Alleviating Hangover and Improving Hepatic Function - Google Patents

Abstract

The present invention relates to a food composition comprising a Mesembryanthemum crystalinum extract, a Pueraria lobata flower extract, and an Artemisia indica extract, wherein when ingesting the food composition of the present invention, the present invention has an effect of promoting alcohol metabolism and removing a hangover and protecting a liver function due to protecting the liver cells from alcohol.

Description

Food composition for relieving hangover and improving liver function containing ice plant extract, brown flower extract, and mugwort extract

The present invention relates to a food composition for relieving hangover and improving liver function, comprising an ice plant extract, a lard extract, and a mugwort extract.

Unlike the existing appearances made through dinner parties and various gatherings, a new concept of drinking culture, such as drinking alone and drinking at home, has emerged through the Corona era. Due to this culture, sales of alcohol are steadily increasing. In addition, Grand View Research, a market research and consulting agency based in San Francisco, California, USA, announced on the 21st that 'hangover cure market size by product, supply route, region, and area, market share and trend analysis and According to the 2021-2028 Outlook report, the hangover cure market is expected to reach $4.67 billion in 2028 with continued growth.

A hangover is a symptom that appears after consuming a large amount of alcohol. It refers to symptoms such as headache, diarrhea, anorexia, nausea, vomiting, chills, and cold sweat. it means. It is known that the cause of a hangover is dehydration, toxicity of alcohol and alcohol metabolites such as acetaldehyde, formaldehyde, and acetone, and deficiency of nutrients such as blood sugar, vitamins, or minerals due to malabsorption.

After drinking, alcohol is metabolized through three pathways. When the concentration of ethanol is low, it is metabolized into alcohol dehydrogenase, acetaldehyde and acetic acid present in the gastrointestinal tract or liver, and then through the action of catalase present in the peroxisome to carbon dioxide and water. It breaks down. If more than an appropriate amount of alcohol is introduced, the balance of the metabolic system described above is destroyed, and body homeostasis cannot be maintained, resulting in headaches or heaviness, loss of concentration, heartburn and indigestion in the short term, and liver dysfunction in the long term. there is. Therefore, a hangover remover capable of relieving hangover and protecting liver function is being continuously studied.

Republic of Korea Patent Registration No. 10-0574097 (2006.04.27)

One aspect of the present invention is to provide a food composition for relieving hangover or improving liver function, comprising an ice plant ( Mesembryanthemum crystalinum ) extract, a gall flower ( Pueraria lobata flower) extract, and a mugwort ( Artemisia indica ) extract.

One aspect of the present invention provides a food composition for relieving hangover or improving liver function, comprising an ice plant ( Mesembryanthemum crystalinum ) extract, a gall flower ( Pueraria lobata flower ) extract, and a mugwort ( Artemisia indica ) extract.

The ice plant ( Mesembryanthemum crystalinum ) is a succulent plant of the genus Mesembryanthemum, and lives mainly in the Namib Desert of South Africa.

The galhwa ( Pueraria lobata flower) means the flower of kudzu, and is also referred to as 'gyeje', 'nokgwak', and 'hwanggeun'.

The mugwort ( Artemisia indica ) is one of the perennial plants belonging to Asteraceae.
The mugwort may be mugwort whose scientific name is Artemisia indica .

According to one embodiment, the weight ratio of the ice plant extract, the brown flower extract, and the mugwort extract may be 1:2:2 to 1:4:4. For example, the weight ratio of the ice plant extract, the brown flower extract, and the mugwort extract may be 1:3:3.

According to one embodiment, the extract may be independently extracted with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. For example, it may be extracted with water.

The extract may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying the ice plant extract, lard extract, and mugwort extract of the present invention in the art.

The hangover is a symptom that appears after consuming a large amount of alcohol, and refers to symptoms such as headache, diarrhea, anorexia, nausea, vomiting, chills, cold sweat, etc. It could mean change.

The hangover relief may be to alleviate symptoms such as headache, diarrhea, anorexia, nausea, vomiting, chills, cold sweat, or symptoms of cognitive decline, decreased exercise capacity, hematological changes and hormonal changes.

The hangover relief may occur through a process in which alcohol metabolism is rapidly progressed because the composition including the ice plant extract, the gall flower extract, and the mugwort extract increases ADH and ALDH activities.

The improvement of liver function may be suppression of liver cell destruction due to alcohol, or suppression of liver function deterioration due to alcohol.

According to one embodiment, the food may be in the form of a beverage, pill, tablet, capsule, jelly, fermented milk, candy, or ice cream.

When the composition of the present invention is prepared as a food composition, in addition to the ice plant extract, the brown flower extract, and the mugwort extract, as active ingredients, it may include ingredients commonly added during food preparation, for example, proteins, carbohydrates, It may contain fats, nutrients, seasonings and flavors. Examples of carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrins and cyclodextrins, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents (thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used.

For example, when the food composition of the present invention is prepared as a drink, citric acid, high fructose corn syrup, sugar, glucose, acetic acid, malic acid, fruit juice, Eucommia extract, jujube extract, in addition to the ice plant extract, lard extract, and mugwort extract of the present invention And / or licorice extract and the like may be further included.

In addition, the food composition of the present invention is various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and It may contain salts thereof, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, and the like.

These components may be used independently or in combination, and the ratio of these additives may be selected in the range of 0 to about 20 parts by weight per 100 parts by weight of the food composition of the present invention, but is not limited thereto.

Ingestion of the food composition containing the ice plant extract, lard extract, and mugwort extract of the present invention promotes alcohol metabolism and protects liver cells from alcohol, thereby suppressing hangover and protecting liver function.

In the drawings of the present invention, NA (Normal administered) or Control is the control (untreated) group, Alcohol is the ethanol 3g/kg administration group, GSH-YE is ethanol 3g/kg and GSH-YE (yeast extract rich in glutathione) 70mg /kg administration group, M. crystallinum ethanol 3g/kg and ice plant extract 70mg/kg administration group, P. lobata flower ethanol 3g/kg and brown flower extract 70mg/kg administration group, A.indica ethanol 3g/kg and The mugwort extract 70mg/kg administration group and HEM (Herbal extract mixture) mean the ethanol 3g/kg and ice plant extract, brown flower extract, and mugwort extract mixture 70mg/kg administration group.
1 is a graph showing the ADH (a) and ALDH (b) activities of each group.
2 is a graph showing the radical scavenging activity according to the concentration of each group.
Figure 3 is the experimental data confirming the GABA content of the ice plant extract ( M. crystallinum ), brown flower extract ( P. lobata flower), and mugwort extract ( A. indica ).
Figure 4 is a graph (a) showing the measured serum ethanol concentration of each group at 0, 0.5, 1, 3, and 5 hours, and a graph (b) showing the calculated AUC, which is the value of the serum alcohol concentration time curve.
5 is a graph (a) showing the concentration of acetaldehyde in the serum of each group measured at 0, 0.5, 1, 3, and 5 hours, and a graph (b) showing the calculated AUC, which is the value of the time curve of the acetaldehyde concentration in the serum.
6 is data showing ADH enzyme activity (a), ALDH enzyme activity (b), SOD enzyme activity (c), and GPx enzyme activity (d) of each group.
7 is data confirming the expression of hepatocyte genes in each group, and data confirming the expression levels of ADH4, ADH6, and ALDH2 genes representing metabolic abilities and GPX2 and CYP2E1 genes representing antioxidant capabilities.
8 is data confirming the expression levels of TGF-β, SMAD-4, and α-SMA, which are genetic indicators indicating liver damage, in each group.

Hereinafter, one or more specific examples will be described in more detail through examples. However, these examples are intended to illustrate one or more specific examples, and the scope of the present invention is not limited to these examples.

Example 1: Experimental procedure

1-1. Reagent preparation and equipment

AHD, ALDH and ascorbic acid, 2,2-diphenyl-1-picrylhydrazyl, and GABA were purchased from Sigma Aldrich. Amino acid mixed standard solution (type H) was purchased from Wako Pure Chemical Industries, Ltd. GABA analysis was performed with an amino acid analyzer equipped with a visible detector (L-8900 system: Hitachi Inc). In the experiment, S9 rat liver homogenase (MOLTOX Co) serum ADH, ALDH, SOD, and glutathione peroxidase ELISA kit (Cayman Chemical) were used. Yeast extract rich in glutathione was purchased from Zhe-jiang Senyo Biotech (Huzhou City, China).

1-2. Preparation of herbal extracts and HEM (Herbal extract mixture)

Dried ice plant ( Mesembryanthemum crystalinum ), brown flower ( Pueraria lobata flower), and mugwort ( Artemisia indica ) were purchased from Human Herb (Humanherb, Daegu, Korea), and each herb (ice plant, brown flower, mugwort) was incubated at 90 ° C. was extracted with about 10 times the weight of water or more for 6 hours. Since the obtained extract was concentrated in a rotary vacuum evaporator at 55 to 65 ° Bx for 12 to 24 hours, an ice plant extract, a brown flower extract, and a mugwort extract were prepared.

HEM (Herbal extract mixture) was a mixture of the ice plant extract, lard extract, and mugwort extract in a ratio of 1:3:3. Concentrated materials were used after being dissolved in water again for the experiment.

1-3. HPLC analysis of organic acids and flavonoids

Organic acids and flavonoids of HEM were measured using an Agilent 1280 HPLC system (agilent Technologies, St. Louis, MO, USA) equipped with a UV detector. Chromatographic separation of flavonoids was analyzed using a CAPCEL PAC C18 UG120 (4.6×250 mm, 5 μm, SHISEIDO). The Alltech permous OA column was used to analyze malic acid, maleic acid, succinic acid, acetic acid, and propionic acid at a wavelength of 540 nm, and the Bio-rad Aminex HPx-87H column was used to analyze butyric acid and tartaric acid at a wavelength of 210 nm. Flavonoids were detected at a wavelength of 370 nm, 25 mM phosphate buffer was used as a mobile phase for an Alltech proof OA column, and 0.01 N sulfuric acid solution was used for a Bo-rad Aminex HPX-87H column. The flavonoid analysis mobile phase consisted of 0.1% phosphoric acid in acetonitrile (solvent A) and 0.1% phosphoric acid in water (solvent B), and all mobile phases were set at 1 mL/min.

1-4. Chromatographic methods for amino acid analyzers

For GABA analysis, an amino acid analyzer (L-8900 system, Hitachi) equipped with a visible detector was used. 0.2 g of the sample was thoroughly mixed with 3 ml of 6 M HCl in a 15 ml test tube and sealed. The mixture was subjected to acid hydrolysis at 110° C. for 24 hours in a heating block (Thermo-Fisher Scientific Co., Rockford, IL, USA).

After mixing 10 μl of the hydrolyzed sample with 990 μl of sodium dilution buffer (pH 2.2), it was filtered through a 0.22 μm membrane filter. The contents of GABA and glutamate were obtained by calculating peak areas, which were compared with standard solutions.

1-5. Measurement of ADH (alcohol dehydrogenase) and ALDH (acetaldehyde dehydrogenase) activation

Measurements of ADH and ALDH activation were reported in a paper [Bostian et al. 1987] was modified and used, and the rate of NADH production at 340 nm absorbance was used as an indicator.

For ADH measurement, 0.1 ml, 0.15 ml of S9 SD rat liver homogenate emulsified in 1.4 ml of distilled water in 0.75 ml of 1.0 M tris-HCl buffer (pH 8.8), 0.3 ml of 20 mM NAD+, 0.3 ml of ethanol, 0.1% bovine serum albumin Samples were mixed in a maximum 3 ml cuvette. The solution was incubated for 5 minutes and then measured at 340 nm.

For ALDH measurement, 2.1 ml of distilled water, 1.0 M tris-HCl buffer (pH 8.0), 0.1 ml of 20nm NAD+, 0.1 ml of 0.33 M 2-mercaptoethanol, 0.1 ml of S9SD rat liver homogenate, and 0.1 ml of sample were measured. did As the samples, untreated group, glutathione-rich yeast extract (GSH-YE), ice plant extract, brown flower extract, mugwort extract, and a mixture of ice plant, brown flower, and mugwort extract (HEM) were used as the control group.

1-6. Measurement of DPPH radical scavenging activity

An ethanol solution with DPPH of 0.36 mM was prepared and equal volumes of samples were mixed.

The mixed solution was incubated for 45 minutes and then measured at 517 nm. As the sample, ascorbic acid was used as a control, and GSH-YE, each extract, and HEM were used.

DPPH scavenging activity was calculated using Equation 1 as follows.

[Equation 1]

DPPH radical scavenging activity (%) = {Abs _control - ₍ Abs _sample - Abs _{sample blank)} }/ Abs _control ×100

1-7. animal

SD rats (male, 7 weeks old, 200-250 g) were purchased from DBL (Korea) and used. The rats were housed in a laboratory provided with an environment of 20-23° C. and a 12-hour light/dark cycle, were fed a standard autoclaved laboratory diet, and had free access to water. Borden experimental procedures were approved by the CHAON Ethics Review Board (CE2128).

1-8. Pharmacokinetic studies

The protocol for pharmacokinetics was performed by slightly modifying the previously known method. Briefly, male SD rats were randomly divided into 4 groups of 8 rats as shown in Table 1 below. Rats were orally administered 3 g/kg of ethanol 30 minutes after administration of GSH-YE or HEM. HEM was composed of a mixture of ice plant extract, brown flower extract, and mugwort extract in a ratio of 1:3:3, and the final concentration was 70 mg/kg. Blood samples were collected through the jugular vein of rats 0, 0.5, 1, 3, and 5 hours after administration, and then centrifuged at 3000 g for 30 minutes to separate serum. The kinetics of the drug were studied by analyzing the separated serum in the following manner.

group name Dosage content control group untreated alcohol group Ethanol 3 g/kg administration GSH-YE Group Ethanol 3 g/kg and GSH-YE 70 mg/kg administered HEM group Ethanol 3 g/kg and HEM 70 mg/kg administered

1-8-1. Measurement of alcohol concentration and acetaldehyde concentration in blood

Blood alcohol concentration was measured using an alcohol measurement kit (#332-UV, Sigma Chemical Co., St. Louis, USA). After mixing 10 ul of serum and 3 ml of NAD-ADH solution, incubation was performed at 30° C. for 10 minutes, and the change in absorbance according to the concentration of NADH generated at a wavelength of 340 nm was measured. At this time, the blood alcohol concentration (g/L) was calculated using a standard solution. The concentration of acetaldehyde in the blood is measured by a kit (Roche Co., USA) manufactured on the principle that acetaldehyde generates acetate by ALDH and NADH is produced in the presence of NAD+, and the concentration of generated NADH is measured at a wavelength of 340 nm. It was measured using

1-8-2. Measurement of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) concentrations in the blood

Alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) enzyme activities in the blood were measured using the alcohol dehydrogenase activity assay kit and aldehyde dehydrogenase activity assay kit purchased from Biovision (Milpitas, CA, USA), as suggested by the manufacturer. It was measured from serum separated according to.

1-8-3. Measurement of SOD and GPx activity in blood

SOD activity and GPx activity, which are antioxidant enzymes, were measured using a colorimetry assay. SOD activity was measured using the Superoxide Dismutase assay Kit (#706002, Cayman chemical company, Michigan, USA) according to the manufacturer's instructions, and was measured at 450 nm. GPx activity was measured using Glutathione Peroxidase Assay Kit (#703102, Cayman chemical company, Michigan, USA) according to the manufacturer's instructions, and was measured at 340 nm.

1-9. Gene expression analysis in liver and lung tissues

Total RNA was extracted using the protocol of the Easy-spin ^TM RNA kit. Liver tissue samples were homogenized in Matrix D tubes using Fast-prep®-24 lysis buffer. After the tissue was minced, the rest of the protocol from the Easy-spin ^TM RNA kit was followed. Total RNA was stored at -70°C for subsequent gene expression analysis. Total RNA was reverse transcribed into cDNA using the Omniscript® RT kit. cDNA was amplified with Gene Expression Master Mix and TaqMan Probe on a Quant Studio ^TM 6 Flex Real-Time instrument. 정량화는 Taqman primer, ADH4(Rn00568609_m1), ADH6(Rn01522017_s1), ALDH2(Rn00583474_m1), GPX2(Rn00822100_GH), CYP2E1(Rn00580624_m1), GAPDH(Rn01775736_g1), TGF-β(Rn00572010_m1), SMAD-4(Rn00570593_m1), α -smooth muscle actin (α -SMA, Rn01759928_g1) was used.

1-10. statistical analysis

All data are expressed as mean ± standard error. Significant differences were statistically analyzed using one-way ANOVA followed by Duncan's multiple range test (p<0.05). The obtained data was analyzed using the GraphPad Prism 5 program (GraphPad Software, Inc.).

Example 2: Experimental results

2-1. Determination of ADH and ALDH activities of extracts and mixtures

In order to confirm the ADH and ALDH activities of the herb extract prepared in Example 1, and to find a ratio showing a significant ADH and ALDH activity, ADH and ALDH activity confirmation experiments were performed by mixing each extract.

First, it was confirmed whether a synergistic effect was exhibited when the brown flower extract and mugwort extract, which exhibited more marked ADH activity, were mixed, or a more pronounced effect was obtained when each ratio was adjusted. As a result, as shown in Table 2 below, it was confirmed that when the brown flower extract and the mugwort extract were mixed at a ratio of 1:1, the effect was significantly higher than that of other ratios. Therefore, the mixing ratio of the brown flower extract and the mugwort extract was determined to be 1:1.

Then, in order to confirm the optimal ratio of the ice plant extract, the brown flower extract and the mugwort extract, they were mixed in the ratio shown in Table 2 below, and the ADH and ALDH activities were confirmed. As a result, as shown in Table 2 below, it was confirmed that the ADH activity and the ALDH activity significantly increased in the mixture in which the brown flower extract and the mugwort extract were mixed 3 times more than the ice plant. Therefore, the ratio of the ice plant extract, the brown flower extract, and the mugwort extract of HEM was determined to be 1:3:3.

2-2. In vitro assay of ADH and ALDH activity

Bovine serum albumin solution 0.1 % S9 rat liver homogenate was used.

As shown in Figure 1A, it was confirmed that the ADH enzyme activity was improved not only in the GSH-YE group but also in the natural herb extract treatment group. As shown in FIG. 1B, the ice plant extract was significantly improved than other herbal extracts, and showed the ALDH activity assay similar to that of the positive control, GSH-YE. In addition, as shown in FIG. 1, it was confirmed that HEM showed a synergistic effect in both ADH and ALDH activities by 157.89 ± 1.28% and 125.92 ± 9.72%.

2-3. DPPH free radical scavenging activity assay

In order to confirm the antioxidant effect of each herb extract and HEM, DPPH free radical scavenging activity was measured.

As shown in FIG. 2, it was confirmed that HEM and mugwort extract had the most significant effects on DPPH free radical scavenging activity, and that brown flower extract, mugwort extract, and HEM exhibited concentration-dependent antioxidant effects.

2-4. GABA content analysis

GABA contained in ice plant extract, brown flower extract, and mugwort extract was analyzed using an automatic amino acid analyzer. As shown in Figure 3, the GABA content of the ice plant extract was 3.02 ± 0.25 mg / g, significantly higher than the GABA content of the brown flower extract of 1.16 ± 0.11 mg / g and the GABA content of the mugwort extract of 0.92 ± 0.16 mg / g. confirmed that

2-5. Chemical composition analysis of HEM, organic acids and flavonoids, amino acids

Chemical components, organic acids, and flavonoids contained in HEM were analyzed by HPLC.

The measured organic acid content is shown in Table 3 below, the flavonoid content is shown in Table 4 below, and the amino acid (GABA) content is shown in Table 5 below. HEM showed high content of succinic acid among organic acids, which was the highest in mugwort extract. In planoids, the content of quercetin was high, and the highest amount was found in brown rice.

Oranic acid HEM (mg/g) M. crystallinum
(mg/g) P. lobata flower
(mg/g) A. indica
(mg/g) Malic acid 9.38 ± 1.78 20.44 ± 2.29 8.82 ± 1.71 6.25 ± 1.80 maleic acid 0.09 ± 0.03 0.06 ± 0.01 0.13 ± 0.05 0.07 ± 0.04 Succinic acid 30.49 ± 3.48 11.07 ± 0.04 5.27 ± 0.02 62.19 ± 7.48 Acetic acid 13.91 ± 4.63 12.59 ± 3.63 17.54 ± 5.72 10.72 ± 3.16

Flavonoids HEM (mg/g) M. crystallinum
(mg/g) P. lobata flower
(mg/g) A. indica
(mg/g) Quercetin 0.69 ± 0.09 0.19 ± 0.08 1.38 ± 0.24 0.17 ± 0.09 Kaemferol 0.27 ± 0.04 ND 0.62 ± 0.13 ND (Non-detected) Isorhamnetin 0.09 ± 0.02 0.05 ± 0.001 0.05. ± 0.02 0.14 ± 0.07

Amino acids HEM (mg/g) M. crystallinum
(mg/g) P. lobata flower
(mg/g) A. indica
(mg/g) GABA 0.69 ± 0.09 0.19 ± 0.08 1.38 ± 0.24 0.17 ± 0.09

2-6. Confirmation of the effect of HEM on the concentration of ethanol in serum

It was confirmed whether HEM inhibits ethanol absorption and prevents hangover symptoms.

As shown in FIG. 4, the change in ethanol concentration in rats of each group was confirmed and shown in FIG. 4A, and the area under the serum concentration time curve (AUC) value was calculated and shown in FIG. 4B. Blood was collected for 5 times, 0, 0.5, 1, 3, and 5 hours after oral administration of 3 g/kg of ethanol in rats. Serum ethanol concentration in all groups decreased continuously after reaching the maximum concentration at 0.5 hour. As shown in FIG. 4, it was confirmed that the serum ethanol concentration in rats administered with HEM was the lowest compared to the same time period. In addition, as shown in Table 6 below, the AUC values of ethanol in the alcohol, GSH-YE, and HEM groups were 2.583 ± 0.541, 1.541 ± 0.465, and 0.796 ± 0.188 g/L*h, respectively. It was confirmed that the peak serum ethanol concentration (Cmax) value in the HEM administration group decreased significantly compared to the alcohol group.

Parameters Alcohol GSH-YE HEM AUC (g/L*h) 2.583 ± 0.541 1.541 ± 0.465 0.796 ± 0.188 Cmax (g/L) 0.726 ± 0.119 0.439 ± 0.079 0.218 ± 0.069 Tmax (h) 0.563 ± 0.177 0.562 ± 0.176 1.1±0.699

2-7. Confirmation of the effect of HEM on the concentration of acetaldehyde in serum

After administration of ethanol, the concentration of acetaldehyde in serum was checked about 5 times, and as in Examples 2-6, it was collected at 0, 0.5, 1, 3, and 5 hours.

As shown in FIG. 5, the concentration of acetaldehyde in serum was the lowest in rats administered with HEM, and in the case of rats administered with HEM, the serum concentration of acetaldehyde exceeded 0.1 g/L, and the measured time was longer. found out that there was no Acetaldehyde pharmacokinetic parameter results are shown in Table 7 below.

Parameters Alcohol GSH-YE HEM AUC (g/L*h) 1.003 ± 0.214 0.688 ± 0.084 0.457 ± 0.157 Cmax (g/L) 0.341 ± 0.086 0.239 ± 0.026 0.125 ± 0.043 Tmax (h) 0.563 ± 0.002 0.5 ± 0.176 1.3 ± 0.918

2-8. Alcohol metabolizing enzymes (ADH and ALDH) and antioxidant enzymes (SOD and GPx) activities

For HEM studies on enzyme activity, alcohol metabolizing enzymes and antioxidant enzymes were identified.

As shown in FIGS. 6A and 6B , it was confirmed that the activities of the ADH enzyme and the ALDH enzyme were significantly higher in the GSH-YE group and the HEM group than in the Alcohol group. Specifically, it was confirmed that the ADH enzyme activity in the HEM group was about 1.75 times higher than that of the Alcohol group, and the ALDH enzyme activity was about 4.09 times higher than that of the Alcohol group.

As shown in Figures 6C, 6D and Table 8, the enzyme activity in the serum was confirmed to be higher in the GSH-YE group and the HEM group compared to the Alcohol group, similar to that of the N.A group, which is a control group (untreated group). It was confirmed that the numerical value was indicated. In addition, since the HEM group showed higher values than the N.A group, it was confirmed that HEM activates SOD and GPx enzymes more.

Parameters N.A. (Control) Alcohol GSH-YE HEM SOD (U/L) 10.12 ± 0.34 6.60 ± 0.82 9.06 ± 1.71 10.79 ± 2.40 GPx (U/L) 26.11±2.15 14.04±4.15 26.31±4.49 27.46 ± 1.51

2-9. Analysis of gene expression in liver cells

Gene responses to ethanol administration were confirmed in rat liver tissue.

ADH4, ADH6, and ALDH2 genes were confirmed for metabolic ability, and GPX2 and CYP2E1 genes were confirmed for antioxidant ability.

As shown in Figure 7, it was confirmed that the gene expression levels of the AHD4, ADH6, ALDH2, and GPX2 genes decreased in the Alcohol group compared to the untreated group, and increased in the GSH-YE and HEM groups rather than the untreated group. . In addition, CYP2E1, an oxidative stress-related gene, increased in gene expression in the Alcohol group compared to the untreated group, and decreased gene expression in the GSH-YE and HEM groups compared to the Alcohol group.

In addition, since the TGF-β/SMAD signaling pathway and α-SMA can induce liver damage and inflammation in alcohol, the TGF-β/SMAD signaling pathway and α-SMA inhibitory action alleviates liver damage.

As shown in Figure 8, the expression levels of TGF-β, SMAD-4, and α-SMA genes were significantly increased in the Alcohol group, and the expression of TGF-β, SMAD-4, and α-SMA genes in the GSH-YE and HEM groups It was confirmed that the amount was significantly decreased compared to the Alcohol group. In addition, in the case of the HEM group, it was confirmed that the TGF-β, SMAD-4, and α-SMA gene expression levels were lower than that of the non-treated N.A group.

So far, the present invention has been looked at with respect to its preferred embodiments. Those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent scope will be construed as being included in the present invention.

Claims (4)

A food composition for relieving hangover or improving liver function,
The weight ratio of the ice plant extract, the gall flower extract, and the mugwort extract having the scientific name Artemisia indica is 1: 2: 2 to 1: 4: 4,
A food composition for relieving hangover or improving liver function.
delete According to claim 1,
The extracts are each independently extracted with water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof,
A food composition for relieving hangover or improving liver function.
According to claim 1,
The food is in the form of a beverage, pill, tablet, capsule, jelly, fermented milk, candy, ice cream,
A food composition for relieving hangover or improving liver function.

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