KR102505375B1 - A cosmetic composition that provides an improved trouble-relieving effect and a method of manufacturing the same - Google Patents

Abstract

Disclosed is a cosmetic composition for soothing the skin, which contains a Lactobacillus antler fermentation filtrate, a bifidus fermentation lysate, and a Jericho rose extract as active ingredients. The cosmetic composition according to one embodiment improves skin soothing and has a moisturizing effect, and furthermore, the cosmetic composition according to the prescription provides excellent formulation stability.

Description

A cosmetic composition that provides an improved trouble-relieving effect and a method of manufacturing the same}

The following examples relate to a cosmetic composition that provides an improved trouble soothing effect and a manufacturing method technology thereof.

In general, sensitive skin has a moderate amount of sebum secretion, but is sensitive to external stimuli and is prone to inflammation. It is more sensitive than normal skin to small physical and chemical changes or stimuli, environmental external factors, and physiological internal changes. Responsive skin. Diseases caused by such sensitive skin include eczema, rosacea, contact dermatitis, and the like, and among them, atopic dermatitis is the most representative disease.

Atopic dermatitis is a very itchy chronic inflammatory dermatitis, a typical skin disease that appears in people with allergies. is increasing It is said that about 50% of cases disappear within two years, 25% disappear during adolescence, and the remaining 25% do not disappear even after becoming an adult. Also, in recent years, the number of cases where atopy develops for the first time as an adult due to environmental factors and stress is rapidly increasing. According to the domestic medical community, about 15% of the entire population of Korea suffers from atopic dermatitis.

It starts with dry skin as the moisture of the skin is not protected, accompanied by extreme itching, turns red, and becomes itchy when the skin swells. Accordingly, if the skin is repeatedly scratched, it develops into an inflammatory disease by the bacteria between the nails, and in severe cases, it is a disease that can cause exudation and scabs.

Inflammatory response is a mechanism to restore and regenerate the damaged area when a living organism or tissue is subjected to invasion that causes any organic change, such as physical action, chemical substance, or bacterial infection. Once stimulation is applied, histamine, serotonin, Vasoactive substances such as bradykinin, prostaglandin, hydroxyleicosatetraenoic acid (HETE), and leukotrienes are released, resulting in increased vascular compaction and inflammation.

Like most diseases, skin diseases also accompany inflammatory reactions, and representative skin diseases such as allergic contact dermatitis, psoriasis, and atopic dermatitis are inflammatory diseases mediated by immune cells, T cells. Therefore, anti-inflammatory drugs must be applied to the management and treatment of major skin diseases. Steroidal anti-inflammatory agents are used to treat these inflammatory diseases, but steroidal agents have many side effects, limiting their use. Non-steroidal anti-inflammatory drugs can also be obtained from natural plant resources, like natural antibacterial agents, and many studies have already been conducted not only in oriental medicine and traditional Chinese medicine, but also in western medicine.

The present invention is to solve the above problems, and it was confirmed that the mixed fermented extract designed by the present inventors has a soothing effect on the skin, and the optimal formulation for this was developed to complete the present invention.

KR100994987B1 KR20160068327A

Embodiments are a cosmetic composition for improving skin troubles, to provide a mixed fermented extract and to provide an optimal formulation for active ingredients.

As one aspect for solving the above problems, the present invention

Provided is a cosmetic composition for skin soothing, comprising a lactobacillus deer antler fermented filtrate, a bifidus fermented lysate, and a rose extract of Jericho as active ingredients.

According to one embodiment,

The lactobacillus deer antler fermentation filtrate is

(a) culturing by adding 8 to 12 parts by weight of distilled water to 1 part by weight of deer antler and then adding culture solutions of Lactobacillus acidophillus, Lactobacillus casei and Lactobacillus ruteri ;

(b) centrifuging the culture medium and filtering the supernatant through filter paper;

(c) concentrating and drying the filtrate to obtain fermented antler;

(d) adding 8 to 12 parts by weight of distilled water to 1 part by weight of the fermented deer antler and extracting at a temperature of 70 to 90 ° C; and

(e) it may be prepared by including the step of filtering the extracted material and concentrating the filtrate under reduced pressure.

According to one embodiment,

The bifidus fermentation lysate is

(A) inoculating a Bifidobacterium bifidum strain into a medium containing 90 to 110 g/L of skim milk powder and culturing for 2 to 4 days;

(B) obtaining a supernatant by centrifuging the culture medium;

(C) disrupting the cells in the supernatant and filtering to obtain a filtrate; and

(D) mixing the filtrate with distilled water at a weight ratio of 1:0.8 to 1.2.

According to one embodiment, the rose of Jericho extract

(i) adding 8 to 12 parts by weight of aqueous ethanol solution to 1 part by weight of pulverized dried leaves and stems of Jericho rose, followed by ultrasonic extraction;

(ii) it may be prepared by including the step of filtering the extract under reduced pressure.

According to one embodiment, the cosmetic composition is prepared by mixing the water phase part, the oil phase part, and the gelling solution,

The aqueous phase part contains 1.3 to 1.7% by weight of the Lactobacillus deer antler fermented filtrate, 0.8 to 1.2% by weight of the bifidus fermented lysate, and 2.3 to 2.7% by weight of the rose extract of Jericho,

The oily phase contains 0.8 to 1.2% by weight of dimethicone, 1.8 to 2.2% by weight of ethylhexylglycerin, and 3.6 to 4.4% by weight of tocopheryl acetate,

The gelling solution may contain 2.7 to 3.3 wt% of ethylhexyl palmitate and 1.8 to 2.2 wt% of calcium pantothenate.

An apparatus according to an embodiment may be combined with hardware and controlled by a computer program stored in a medium to execute any one of the methods described above.

In the examples, Lactobacillus antler fermented filtrate, bifidus fermented lysate, and rose Jericho extract were used as main active ingredients to improve skin soothing and have a moisturizing effect. Furthermore, the cosmetic composition according to the prescription provides excellent formulation stability.

Hereinafter, embodiments will be described in detail with reference to the accompanying drawings. However, since various changes can be made to the embodiments, the scope of the patent application is not limited or limited by these embodiments. It should be understood that all changes, equivalents or substitutes to the embodiments are included within the scope of rights.

Specific structural or functional descriptions of the embodiments are disclosed for illustrative purposes only, and may be modified and implemented in various forms. Therefore, the embodiments are not limited to the specific disclosed form, and the scope of the present specification includes changes, equivalents, or substitutes included in the technical spirit.

Although terms such as first or second may be used to describe various components, such terms should only be construed for the purpose of distinguishing one component from another. For example, a first element may be termed a second element, and similarly, a second element may be termed a first element.

It should be understood that when an element is referred to as being “connected” to another element, it may be directly connected or connected to the other element, but other elements may exist in the middle.

Terms used in the examples are used only for descriptive purposes and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, terms such as "include" or "have" are intended to designate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the art to which the embodiment belongs. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the present application, they should not be interpreted in an ideal or excessively formal meaning. don't

Advantages and features of the present invention, and methods of achieving them, will become clear with reference to the detailed description of the following embodiments taken in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various different forms, only these embodiments make the disclosure of the present invention complete, and common knowledge in the art to which the present invention belongs. It is provided to fully inform the holder of the scope of the invention, and the present invention is only defined by the scope of the claims.

In the embodiments of the present invention, unless otherwise defined, all terms used herein, including technical or scientific terms, are the same as those commonly understood by one of ordinary skill in the art to which the present invention belongs. It has meaning. Terms such as those defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related art, and unless explicitly defined in the embodiments of the present invention, an ideal or excessively formal meaning not be interpreted as

The shapes, sizes, ratios, angles, numbers, etc. disclosed in the drawings for explaining the embodiments of the present invention are illustrative, so the present invention is not limited to the details shown. In addition, in describing the present invention, if it is determined that a detailed description of related known technologies may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted. When 'includes', 'has', 'consists of', etc. mentioned in this specification is used, other parts may be added unless 'only' is used. In the case where a component is expressed in the singular, the case including the plural is included unless otherwise explicitly stated.

In interpreting the components, even if there is no separate explicit description, it is interpreted as including the error range.

The size and thickness of each component shown in the drawings are shown for convenience of description, and the present invention is not necessarily limited to the size and thickness of the illustrated components.

Each feature of the various embodiments of the present invention can be partially or entirely combined or combined with each other, and as those skilled in the art can fully understand, various interlocking and driving operations are possible, and each embodiment can be implemented independently of each other. It may be possible to implement together in an association relationship.

Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and comparative examples. However, these examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.

Preparation Example 1. Preparation of mixed fermented extract for skin soothing

(1) Preparation of Lactobacillus deer antler fermentation filtrate

2 kg of antler was cut and ground to a size of 0.5 cm. After adding 20 ℓ of distilled water and sterilizing at 121 ° C. for 30 minutes, Lactobacillus acidophillus, Lactobacillus casei and Lactobacillus ruteri cultures were inoculated at a concentration of 2% and 3 cultured for days. The culture solution was centrifuged at 3,000 rpm and 15° C. for 10 minutes to remove strains and culture residues. The supernatant was filtered with a filter paper (3M paper), concentrated by heating sterilization at 95° C. for 1 hour, and then spray-dried to obtain 200 g of fermented antler. 200 g of the obtained fermented deer antler was placed in an extraction vessel (Ilshin Lab Co., Ltd, FD8512), and 2 L of water was added thereto, followed by extraction at 80° C. for 60 minutes. Thereafter, the extract was filtered with a filter paper (3M paper) to obtain a liquid extract. The obtained extract solvent was concentrated under reduced pressure with a vacuum evaporator (Sunil EYELA, Rotatory evaporator, N-1N) to obtain 9.6 g of Lactobacillus deer antler fermented filtrate.

(2) Preparation of bifidus fermentation lysate

Bifidobacterium bifidum ( Bifidobacterium bifidum ) Inoculate the strain into skim milk medium (skim milk powder 100g / L, glucose 20g / L, peptone 3g / L, distilled water 1g / L), incubate at 37 ° C. for 3 days, and then centrifuge to obtain the supernatant. The cells were disrupted with a homogenizer and then filtered with a filter paper (3M paper) to obtain a filtrate. A bifidus fermentation lysate was prepared by mixing the filtrate and distilled water in a weight ratio of 1:1.

(3) Preparation of Jericho rose extract

After pulverizing 100 g of dry leaves and stems of Jericho rose ( Anastatica hierochuntica ) using a mixer, 1,000 ml of 40 v / v% ethanol aqueous solution was added, and extracted at room temperature with an ultrasonic extractor for 4 hours. After the first filtration under reduced pressure using a filter paper (Whatman Paper Filter No. 3) and refrigeration for 4 days, when a precipitate was formed, the extract of the rose of Jericho was finally obtained through the second filtration under reduced pressure.

(4) Formulation of the composition

A mixed fermented extract was prepared by combining the Lactobacillus deer antler fermented filtrate, the bifidus fermented lysate, and the Jericho rose extract in the following ratio.

Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Lactobacillus antler fermented filtrate 3mL 5mL - - bifidus fermentation lysate 2mL - 5mL - Jericho Rose Extract 5mL 5mL 5mL 10 mL

Experimental Example 1. Lipoxygenase activity inhibitory effect

Lipoxygenase (5-LO, 5-lipoxygenase) adds molecular oxygen to unsaturated fatty acids having a 1,4- cis,cis -pentadiene (1,4-pentadiene) structure in the molecule, such as linoleic acid, linolenic acid, and arachidonic acid. As an oxidase that generates 1,3- cis, trans -diene 5-hydroperoxide, it is an enzyme involved in the first reaction of leucotriene biosynthesis from arachidonic acid, which is involved in various inflammatory and allergic diseases. Therefore, the anti-inflammatory effect was confirmed by measuring the inhibitory activity of lipoxygenase using SLO (soybean lipoxygenase), which is structurally and mechanically similar to 5-LO. That is, 2 μl of the sample was added to 200 μl of 0.1M Tris buffer, and 500 units of 15-lipoxygenase were added thereto and reacted at 25°C for 5 minutes. After the reaction, linolenic acid dissolved in ethanol was added to a final concentration of 110 μM, mixed, and absorbance was measured at 234 nm for 5 minutes to calculate the lipoxygenase inhibitory activity (%) of the mixed fermented extract compared to the control group to which no sample was added.

Lipoxygenase inhibitory activity (%) Example 1 81.4 Comparative Example 1 53.1 Comparative Example 2 58.9 Comparative Example 3 47.4

As such, when compared at the same dose, the mixed extract had superior lipoxygenase inhibitory activity compared to the Jericho rose extract alone, and in particular, it was confirmed that a synergistic effect was obtained when both fermented extracts were mixed. From this activity, it was determined that the mixed fermented extract of Example 1 had an excellent skin soothing effect, enabling the development of a cosmetic composition.

Experimental Example 2. Antioxidant effect by DPPH scavenging ability

The skin aging process by reactive oxygen species includes lipid peroxidation, DNA oxidation, melanin production, cleavage of connective tissue, and abnormal cross-links. Most of these reactions are radical reactions, which include autoxidation reactions. Antioxidants play a role in blocking autoxidation reactions in vivo. As the ability to donate electrons, the greater the reducing power, the stronger the antioxidant.

As a reagent for measuring the reducing power of antioxidants, there is 1,1-diphenyl-2 picrylhydrazyl radical (DPPH). DPPH exists as a radical of a stabilized structure of the nitrogen center in the compound. It shows a maximum absorption at 517 nm, and when reduced, the absorption disappears at 517 nm. 100 μL of the sample and 100 μL of a 60 μM DPPH solution were dispensed into a 96 well plate, reacted at room temperature for 30 minutes, and then the absorbance was measured to measure the percent decrease in absorbance compared to the control group to which no sample was added.

DPPH inhibitory activity (%) Example 1 93.2 Comparative Example 1 69.8 Comparative Example 2 73.0 Comparative Example 3 61.3

As in Experimental Example 1, when the same total amount was added, the DPPH inhibitory activity of the mixed extract was superior to that of the Jericho rose extract alone, and in particular, it was confirmed that there was a synergistic effect when both fermented extracts were mixed. From this activity, it was determined that the mixed fermented extract of Example 1 had an excellent skin soothing effect, enabling the development of a cosmetic composition.

Preparation Example 2. Preparation of a cosmetic composition based on Example 1 as a prescription

In Table 4 below, the water phase part was heated to 75 ° C, and the oil phase part was heated to 75 ° C. After mixing the heated aqueous phase and the oil phase, an emulsion was prepared by emulsifying at 75 ° C. for 5 minutes with a homomixer. A hydrogel composition was prepared by adding a gelation solution to the prepared emulsion and stirring for 30 minutes while maintaining 75 ° C.

weight% Prescription Example 1 Comparative Prescription Example 1 Comparative Prescription Example 2 Prime Minister Jericho Rose Extract 2.5% 2.5% 2.5% Purified water To 100 To 100 To 100 Niacinamide 5.0% 5.0% 5.0% Lactobacillus deer antler fermentation filtrate 1.5% 1.5% 1.5% bifida fermentation lysate 1.0% 1.0% 1.0% sodium ascorbyl phosphate 2.0% 2.0% 2.0% 1,2-Hexanediol 1.0% 1.0% 1.0% Hydrolyzed Hyaluronic Acid 1.0% 1.0% 1.0% Hydroxyethyl Urea 2.0% 2.0% 2.0% Ascorbic Acid 0.5% 0.5% 0.5% propanediol 0.5% 0.5% 0.5% Pyridoxine HCL 0.5% 0.5% 0.5% arginine 0.5% 0.5% 0.5% sodium hyaluronate 4.0% 4.0% 4.0% carbomer 5.0% 5.0% 5.0% gelation solution Polyglyceryl-10 Laurate 3.0% 3.0% 3.0% Polyglyceryl-10 Stearate 2.0% 2.0% 2.0% Sodium Acetylated Hyaluronate 2.0% 2.0% 2.0% glycerin 5.0% 5.0% 5.0% Ethylhexyl Palmitate 3.0% 5.0% - Calcium Pantothenate 2.0% - 5.0% Sodium Starthioctenyl Succinate 2.0% 2.0% 2.0% pay-per-view Cyanocobalamin 2.0% 2.0% 2.0% panthenol 2.0% 2.0% 2.0% Ethylhexylglycerin 2.0% 2.0% 2.0% Disodium EDT 0.5% 0.5% 0.5% silica 0.5% 0.5% 0.5% Hydrogenated Lecithin 2.0% 2.0% 2.0% Tocopheryl Acetate 5.0% 1.0% 1.0% dimethicone 1.0% 1.0% 1.0% Hydrolyzed Corn Starch 0.5% 0.5% 0.5% maltodextrin 0.5% 0.5% 0.5% xanthan gum 0.5% 0.5% 0.5% Caramel 0.5% 0.5% 0.5% Spices 0.3% 0.3% 0.3%

Experimental Example 3. Physical properties and stability of the formulation

Comparative Prescription Example 1 did not emulsify well and had high viscosity, making it sticky and agglomeration was strong, whereas Comparative Prescription Example 2 and Prescription Example 1 were less sticky and did not show agglomeration. As a result of storing the formulations at 50 ° C for 1 month, the oil phase was separated in Comparative Prescription Examples 1 and 2, whereas the oil phase was not separated in Example 1, so when ethylhexyl palmitate and calcium pantonate were used, excellent formulation stability was found to have

Experimental Example 4. Measurement of skin moisture content

Thirty 30-year-old women were selected as evaluation subjects, the test site, the face, was washed, and the skin was acclimatized under constant temperature and humidity conditions of 20 ± 3 ° C and 40 ± 2% relative humidity. After that, the product cosmetic composition of Prescription Example 1 and competitor A was applied. Thereafter, the amount of transepidermal water loss after 0 minutes, 30 minutes, 60 minutes, 90 minutes, and 120 minutes was measured using Tewameter TM300 from CK electronic (Germany). The experiment was measured 5 times and the average value was calculated, and the results are shown in Table 5 below.

Transepidermal water loss over time (g/h/m2) 0min 60min 120min Prescription Example 1 3.4 5.1 5.5 Company A product 6.7 12.5 15.7

In the case of using the prescription example as described above, it was confirmed that there was a moisturizing effect compared to other competitor's products, and thus a skin soothing effect could be obtained.

Claims (3)

A cosmetic composition for skin soothing, comprising Lactobacillus antler fermented filtrate, bifidus fermented lysate, and Jericho rose extract as active ingredients,
The lactobacillus deer antler fermentation filtrate is
(a) culturing by adding 8 to 12 parts by weight of distilled water to 1 part by weight of deer antler and then adding culture solutions of Lactobacillus acidophillus, Lactobacillus casei and Lactobacillus ruteri ;
(b) centrifuging the culture medium and filtering the supernatant through filter paper;
(c) concentrating and drying the filtrate to obtain fermented antler;
(d) adding 8 to 12 parts by weight of distilled water to 1 part by weight of the fermented deer antler and extracting at a temperature of 70 to 90 ° C; and
(e) filtering the extracted material and concentrating the filtrate under reduced pressure;
The bifidus fermentation lysate is
(A) inoculating a Bifidobacterium bifidum strain into a medium containing 90 to 110 g/L of skim milk powder and culturing for 2 to 4 days;
(B) obtaining a supernatant by centrifuging the culture medium;
(C) disrupting the cells in the supernatant and filtering to obtain a filtrate; and
(D) mixing the filtrate with distilled water at a weight ratio of 1:0.8 to 1.2;
The Jericho rose extract
(i) adding 8 to 12 parts by weight of aqueous ethanol solution to 1 part by weight of pulverized dried leaves and stems of Jericho rose, followed by ultrasonic extraction;
(ii) it is prepared by including the step of filtering the extract under reduced pressure;
The cosmetic composition is prepared by mixing an aqueous phase part, an oil phase part, and a gelling solution,
The aqueous phase part contains 1.3 to 1.7% by weight of the Lactobacillus deer antler fermented filtrate, 0.8 to 1.2% by weight of the bifidus fermented lysate, and 2.3 to 2.7% by weight of the rose extract of Jericho,
The oily phase contains 0.8 to 1.2% by weight of dimethicone, 1.8 to 2.2% by weight of ethylhexylglycerin, and 3.6 to 4.4% by weight of tocopheryl acetate,
The gelation solution is a cosmetic composition for skin soothing, containing 2.7 to 3.3% by weight of ethylhexyl palmitate and 1.8 to 2.2% by weight of calcium pantothenate.


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