KR102500786B1 - Composition of skin external application for promoting skin volume or renewing - Google Patents

Abstract

The present invention relates to a composition for external application for skin for enhancing skin volume or skin elasticity, containing an extract of fin thistle as an active ingredient. More specifically, a composition containing fin thistle extract as an active ingredient can generate adipocytes by promoting regeneration of human adipose-derived stem cells and promoting the differentiation of stem cells into adipocytes. In addition, since the composition containing the fin thistle extract of the present invention as an active ingredient restores not only stem cells but also damaged skin cells, it can be usefully used as a composition for improving skin wrinkles by giving volume and elasticity to the skin.

Description

Composition of skin external application for promoting skin volume or renewing}

The present invention relates to a composition for external application for skin for enhancing skin volume or enhancing skin elasticity, comprising an extract of fin thistle as an active ingredient. It promotes differentiation into fat cells and restores damaged skin cells. With this effect, it can be usefully applied to improve skin wrinkles by giving volume and elasticity to the skin.

Aging of the skin is a continuous process, and there is a difference in the rate of progression depending on genetic and environmental factors.

Human skin is composed of three layers: epidermis, dermis, and hypodermis. The epidermis is composed of keratinocytes, which have a protective function called keratin. The extracellular matrix (ECM) constituting the dermis consists of about 70% collagen fibers, 30% gel-like matrix, and 1% elastin fibers. Subcutaneous fat is composed of pre-adipocytes and mature adipocytes produced by differentiation of pre-adipocytes. It is also performing a function.

As we age, the epidermis and subcutaneous fat layer thin due to internal causes such as hormone secretion and biochemical changes. In addition, the aging process of the skin with wrinkles and loss of elasticity due to the loss of collagen fibers and elastin fibers in the dermal layer proceeds. In this way, changes in the face due to aging appear as deepening wrinkles and a decrease in volume around the cheeks, and it is a skin concern that needs fundamental improvement.

Fat grafting is a method to enhance skin volume and elasticity due to aging. However, in simple fat transplantation, the fat is absorbed into the body and the engraftment rate of the procedure is low (S Eremia et al., Dermatol. Sur. 2000, 26: 1150-1158), so the transplanted fat is quickly lost. Therefore, there is an increasing demand for a method capable of sufficiently increasing the subcutaneous fat layer while solving and replacing the problems of fat transplantation.

Collagen is currently the most widely used raw material for improving skin elasticity. Such collagen is difficult to absorb into the skin due to its large molecular size, and even if the molecule is broken down during the penetration process and reaches the dermis layer in the form of amino acids, it penetrates without losing the original collagen component and has low effectiveness (Zague et al., Arch. Dermatol. Res. 2008, 300: 479-483). In addition, retinol, which activates fibroblasts as a cosmetic raw material to produce large amounts of collagen and elastin to improve skin elasticity, has the disadvantage of causing skin irritation even when applied to the skin in a small amount compared to its efficacy.

As described above, there is an increasing demand for the development of cosmetics that can increase the volume and elasticity of the skin using safe natural vegetable raw materials without skin irritation. The basic structure of the skin is maintained by the subcutaneous fat tissue of the skin, and the volume and strength are influenced. Reinforcing the subcutaneous fat layer due to the increase in the volume of adipose tissue can be another good direction for wrinkle improvement by providing a sense of volume and elasticity to the skin, rather than the previously used method of imparting elasticity to the dermis or epidermis of the outer skin layer. Related papers have been published (Kim WS et al., J. Dermatol. Sci. 2009, 53(2): 96-102; Trottier V et al., Stem Cells. 2008, 26(10): 2713-23; Park BS et al., Dermatol. Surg. 2008, 34(10): 1323-1326). As described above, enhancing the stem cell nature of the skin with safe natural vegetable raw materials and increasing the volume of adipose tissue in the subcutaneous fat layer can be a good solution for improving skin volume and elasticity.

Furthermore, as the surface of the damaged wound is covered by a layer of keratinocytes, a new epidermis is generated and proliferating fibroblasts form collagen, and the wound healing stage of the skin proceeds. It was confirmed that the fin thistle extract promotes the mobility of keratinocytes and fibroblasts, thereby being effective in wound healing.

In order to solve the above problems, the present invention provides a composition for external application for skin for enhancing skin volume or skin elasticity, containing as an active ingredient a fin thistle extract that is non-irritating to the skin and capable of activating adipose-derived stem cells and increasing adipose tissue. intended to provide

In addition, it is an object of the present invention to provide a cosmetic composition or a pharmaceutical composition containing a fin thistle extract.

An aspect of the present invention for achieving the above object is,

It is to provide a composition for external application for skin for enhancing skin volume or enhancing skin elasticity, comprising an extract of fin thistle as an active ingredient.

The composition for external application for skin of the present invention contains fin thistle extract as an active ingredient to enhance the stem cell nature of adipose-derived stem cells and promote the differentiation of fat cells, thereby increasing skin volume and skin elasticity, and is effective in improving skin wrinkles and wound healing. It is less irritating to the skin, safe to the human body, including natural plants, and has no side effects even when used for a long time.

1 is a graph showing the cytotoxicity of human adipose-derived stem cells according to the concentration of fin thistle extract.
2a and 2b are graphs showing the expression patterns of stem cell proliferation marker genes (2a CD44 and 2b CD73) when adipose-derived stem cells subjected to oxidative stress were treated with fin thistle extract.
Fig. 3a is a photograph showing microscopic observation after treatment with fin thistle extract under differentiation conditions into adipocytes and induction of adipose-derived stem cells into adipocyte differentiation, and Fig. 3b is a photograph showing adipose-derived stem cells treated with fin thistle extract. It is a graph quantitatively showing the relative level of adipocyte differentiation of cells.
4a and 4b are graphs showing the expression patterns of stem cell differentiation marker genes (4a aP2 and 4b PPARγ) when adipose-derived stem cells were treated with fin thistle extract.
Figure 5a is a photograph showing that the movement of skin keratinocytes is promoted by the fin thistle extract, observed under a microscope, and Figure 5b is a graph showing the numerical value of the wound area.
Figure 6a is a photograph showing that the migration of skin fibroblasts is promoted by the fin thistle extract under a microscope, and Figure 6b is a graph showing the numerical value of the wound area.

The present invention relates to a composition for external application for skin for enhancing skin volume or skin elasticity, comprising an extract of fin thistle as an active ingredient.

In the present invention, the composition promotes the proliferation and differentiation of adipose-derived stem cells (ADSCs), thereby enhancing stem cell nature and providing fat cells to the subcutaneous fat layer to give volume and elasticity to the skin. and provides an effect of improving wrinkles, and may be provided as a composition for external application to the skin.

In the present invention, fin thistle, which is an active ingredient of the composition for external application for skin for enhancing skin volume or skin elasticity, has the following characteristics.

The fin thistle (Carduus crispus) is a biennial plant belonging to the Asteraceae family, and is native to the temperate regions of Asia and Europe, and also spreads to North America, and is also called fin thistle and Ungeosi. It grows in fields or roadsides, has fin-like wings running vertically, and has sharp thorns. The leaves are alternate, long oval shape, and the tip is pointed. It is deeply divided and has sawtooth with sharp thorns like a stem at the edge. Flowers bloom in June-August, with purple capitulum at the end of each stem. The name of the crude drug is biryeom (飛廉), and it is effective for fever, anti-inflammation, hemostasis, and puffiness. As a pharmacological action, it is used for cold, itching, arthritis, urethritis, and celiac disease.

In one embodiment of the present invention, the fin thistle extract may be included in 0.0001 to 10% by weight, preferably 0.005 to 5% by weight, more preferably 0.01 to 1% by weight based on the total weight of the composition.

When the fin thistle extract is included in less than 0.0001% by weight, the effect of improving skin volume, skin elasticity, and skin wrinkles may be insignificant, and when it is included in more than 10% by weight, the stability of the formulation is lowered, resulting in skin volume and skin elasticity. , the skin wrinkle enhancement effect may be insignificant, and the fragrance may not be good when manufactured as a cosmetic.

In one embodiment of the present invention, the composition may be a composition for external application for skin for enhancing skin volume or skin elasticity, and the skin volume or skin elasticity may be increased by increasing fat cells and adipose tissue. .

The enhancement of the skin volume means that the volume of the skin layer increases and increases, and the shape of the outer surface of the skin is increased in volume, and it is tightened to look healthy, or damaged skin tissue is restored and approaches its original state can mean

Therefore, the increase in skin volume can prevent skin aging such as wrinkles due to loss of skin elasticity as the skin surface is stretched together while the volume of the portion of the skin layer that has been stretched is increased.

The enhancement of skin elasticity means to maintain or strengthen the ability related to skin wrinkles, and collagen, which is a collagen fiber in the dermal layer of the skin, and elastin, which is an elastic fiber, are the main proteins that play such a role, and are in charge of skin elasticity. However, the biosynthesis of collagen is influenced internally and externally by the skin. Specifically, when the cell activity of skin cells is reduced due to natural aging, collagen fiber is reduced, or as an external factor, excessive irradiation of ultraviolet rays or stress increases the expression of decomposing enzymes such as collagen and elastin, resulting in wrinkles of the skin. It increases skin elasticity and reduces elasticity, leading to skin aging.

In one embodiment of the present invention, the composition for external application for skin may be a composition for skin regeneration, skin wrinkle improvement, anti-aging, or promotion of skin wound healing.

The skin regeneration is a broad concept that includes the ability to generate new keratinocytes in response to overall skin aging and wounds caused by the skin epidermis.

The skin wrinkle improvement and anti-aging are denatured by various factors including sunlight, such as collagen fibers and elastic fibers in the dermis, and wrinkles are formed as the skin folds due to skin aging due to a decrease in skin elasticity. , means to maintain or strengthen.

The skin wound treatment proliferates human adipose-derived stem cells, promotes the differentiation of adipose-derived stem cells into adipocytes, and activates adipose-derived stem cells to secrete various cytokines into the culture medium, so that only the outer part of the skin It means that when abrasions that disappear, lacerations that appear when scratched, or skin damage caused by external physical and chemical environments occur, they are quickly restored.

In one embodiment of the present invention, the fin thistle extract can promote the proliferation of adipose derived stem cells (ADSC).

The adipose-derived stem cells may be human adipose-derived stem cells (hADSCs), but are not limited thereto.

The stem cells are pluripotent capable of differentiating into cells of all tissues of an individual, or cells having self-renewal ability that are totipotent, and the stem cells to be cultured are specific It is not limited to stem cells of, and includes both embryonic stem cells and adult stem cells, but preferably human-derived adult stem cells, most preferably human umbilical cord blood-derived mesenchymal stem cells (human cord blood-derived stem cells, CB-MSCs), skin-derived stem cells, or human adipose-derived stem cells (ADSCs).

The human adipose-derived stem cells may be a kind of adult stem cells isolated from human adipose tissue, and adipose tissue can be obtained incidentally during a conventional liposuction process, so that a sufficient amount of stem cells can be easily obtained and cultured. It also has the advantage of being safer than bone marrow harvesting.

In one embodiment of the present invention, the fin thistle extract has an expression level of cluster of differentiation 44 (hereinafter referred to as CD44) and cluster of differentiation 73 (hereinafter referred to as CD 73), which are markers for adipose-derived stem cells. can increase

The increase in the expression levels of CD44 and CD73 means that the activity of adipose-derived stem cells is increased. Therefore, it can have the effect of increasing the volume of the skin by increasing adipose tissue, and is also practical for skin aging and regeneration, and can maximize the therapeutic effect in wound treatment.

In one embodiment of the present invention, the fin thistle extract can promote the differentiation of adipose-derived stem cells into adipocytes.

Here, differentiation refers to a phenomenon in which a less specialized cell develops into a specific cell and changes into a specific type of cell in size or shape, membrane potential, metabolic activity, and response to signals.

The differentiation of adipocytes promotes the differentiation of adipocytes in order to increase the volume of adipose tissue, and refers to a series of processes in which preadipocytes are converted into adipocytes in adipose tissue growth. It happens continuously throughout our lives in our body.

The differentiation into adipose-derived stem cells is the differentiation of pre-adipocytes or mesenchymal stem cells of animals, preferably mammals, into adipocytes, and more preferably the differentiation of human mesenchymal stem cells into adipocytes. , Most preferably, human adipose-derived stem cells differentiate into adipocytes.

In the present invention, the fin thistle extract can be obtained according to a conventional natural product extraction method in which fin thistle is crushed, an extraction solvent is added, precipitated for a certain period of time, stirred at appropriate time intervals, and the obtained solution is filtered and recovered, and the organic solvent It is preferable to extract using .

The organic solvent may be an alcohol having 1 to 4 carbon atoms, preferably at least one selected from the group consisting of methanol, ethanol, propyl alcohol, butyl alcohol, glycerin, propylene glycol and butylene glycol.

The composition for external application for skin containing the extract of fin thistle as an active ingredient is not particularly limited in its formulation depending on the body part, and may be a cosmetic composition or a pharmaceutical composition.

The cosmetic composition is a composition for skin volume, skin elasticity, skin wrinkle improvement, and anti-aging, specifically cosmetic water, gel, pack, nutrient cream, essence, shampoo, oil-in-water (O/W) type and water-in-oil (W/O) It may be selected from types of dosage forms, and may also be transdermal dosage forms such as ointments, gels, creams, and patches.

The pharmaceutical composition is a composition for skin regeneration and skin wound treatment, and may be a transdermal dosage form such as a lotion, ointment, gel, cream patch or spray, but is not limited thereto.

Hereinafter, the present invention will be described in more detail through the following experimental examples. However, the following experimental examples are only for explaining the present invention, and the scope of the present invention is not limited.

< manufacturing example > Preparation of fin thistle methanol extract

For the fin thistle used in this experiment, methanol (99.9%) was used as the extraction solvent, and the extract sample was extracted at 50 ° C in a dried state under reduced pressure. At this time, the weight of the extracted sample was 22.71± per tube. It was 0.51 mg. This sample was dissolved in DMSO and used.

< Experimental example 1> Measurement of cytotoxicity of fin thistle extract

Human adipose-derived stem cells (hADSCs, Invitrogen, USA) were subcultured in a 100mm cell culture dish _and cultured in a CO ₂ incubator at 37°C and 5.0% CO ₂ conditions. .

Human adipose-derived stem cells were treated with fin thistle extract at concentrations of 5, 10, 20, 50, and 100 ppm, and cytotoxicity was confirmed by MTT assay.

As a result of the above experiment, it was confirmed that there was no cytotoxicity at the concentrations of 5, 10, and 20 ppm of the fin thistle extract, and the above results are shown graphically in FIG. 1.

< Experimental example 2> Stem cell labeling of fin thistle extract marker (CD44, CD73) gene expression level measurement

In order to confirm the stem cell enhancement effect of human adipose-derived stem cells, the expression levels of CD44 and CD73, which are positive cell surface protein factors expressed by stem cells, were examined immunologically. 20 ppm of fin thistle extract was added to the medium, and oxidative stress of human adipose-derived stem cells was treated with hydrogen peroxide at a concentration of 200 μM for 24 hours.

Human adipose-derived stem cells were subcultured in a 100 mm cell culture dish, and then transferred to a 12-well plate at an appropriate concentration. After washing the cells with a phosphate buffered saline (PBS), the RNA was isolated using an RNA purification kit (RNA-spin TM kit, Intron biotechnology). cDNA was synthesized from the isolated RNA with a reverse transcription kit (maxime RT premix, Intron biotechnology), and analyzed quantitatively through real-time polymerase chain reaction.

The expression patterns of CD44 and CD73 genes in human adipose-derived stem cells were compared using Cybergreen (Power SYBR Green, Applied Biosystems) reagent, and the expression levels of CD44 and CD73 genes, which are markers of stem cells, were treated with fin thistle extract. , it was found that an increase in The results are shown in Figures 2a and 2b (2a CD44, 2b CD73).

< Experimental example 3> Efficacy and quantitative measurement of promoting differentiation of human adipose-derived stem cells into adipocytes

In order to induce adipocyte differentiation of human adipose-derived stem cells, a stem cell differentiation induction medium was prepared and used. The medium composition is DMEM high concentration glucose (Dulbecco's Modified Eagle's Medium high glucose) medium, 10% fetal bovine serum (feral bovine serum), 1% antibiotic penicillin-streptomycin (penicillin-streptomycin), 0.5mM 3-isobutyl-1 -Contains 3-isobutyl-1-methylxanthine (IBMX), 1uM dexamethasone, and 200uM indomethacin. Stem cell differentiation was induced while the medium composition was replaced every 3 days for 14 days, and when the differentiation was induced into adipocytes, a fin thistle extract was added to the medium at a concentration of 20 ppm. For 14 days, differentiation into adipocytes was analyzed by measuring the absorbance under a microscope and at a wavelength of 510 nm using Oil red O staining for the stem cells induced to differentiate into adipocytes.

As a result, it was confirmed that the fin thistle extract further promoted the differentiation of human adipose-derived stem cells into adipocytes. The above results are shown in Figures 3a and 3b (3a micrograph, 3b absorbance).

< Experimental example 4> Stem cell differentiation of fin thistle extract marker Gene expression level measurement

In order to confirm the differentiation effect of human adipose-derived stem cells into adipocytes, differentiation into adipocytes was induced with the medium composition in <Experimental Example 3>, and 20 ppm of fin thistle extract was added to the medium at the time of differentiation induction. Under these conditions, stem cell differentiation was performed while replacing the medium every 3 days for 14 days. Human adipose-derived stem cells were subcultured in a 100 mm cell culture dish, transferred to a 12-well plate at an appropriate concentration, and RNA was isolated in the same manner as in <Experimental Example 2>.

cDNA was synthesized from the isolated RNA with a reverse transcription kit (maxime RT premix, Intron biotechnology), and analyzed quantitatively through real-time polymerase chain reaction.

Expression patterns of aP2 and PPARγ genes in human adipose-derived stem cells were compared using Cybergreen (Power SYBR Green, Applied Biosystems) reagent. It was confirmed that the expression level increased. The results are shown in FIGS. 4a and 4b (4a aP2, 4b PPARγ).

< Experimental example 5> Increased migration of human keratinocytes

In order to confirm the wound healing efficacy of the fin thistle extract, the wound healing activity was confirmed after applying a wound to keratinocytes.

Human keratinocyte HaCaT cells were cultured in a 75 cm ² T-flask, and 5.0 × 10 ⁵ cells/ml of human keratinocyte HaCaT cells were transferred to a 6-well plate and the cell monolayer had to be scratched. For a regular interval, a 2-well insert ( Culture-Insert 2 well, ibidi, Germany) was used. 3.5×10 ⁴ cells/70 ul of HaCaT cells were put into each well of _{the insert attached to the 6-well plate, and cultured in a CO 2 incubator} for 24 hours. Culture conditions were 37 ℃, 5.0% CO ₂ conditions.

After 24 hours of culture, the 2-well insert was removed with sterilized tweezers, and the culture medium of HaCaT cells was replaced with a new culture medium. The basic culture medium was treated as a control group, and the culture medium supplemented with 20 ppm of fin thistle extract was treated as an experimental group for 12 hours.

As a result, as shown in FIGS. 5A and 5B , it was confirmed that when the fin thistle extract was added, the wound area was reduced and the migration of keratinocytes was increased. FIG. 5a is a photograph of a wound area compared with a microscope, and FIG. 5b is a result obtained by obtaining an area ratio using an Image J program (Java-based image processing program) and organizing the result into a graph. Through these results, it was confirmed that the fin thistle extract promotes the migration of keratinocytes and has higher wound healing activity than the control group.

< Experimental example 6> Increased migration of human fibroblasts

In order to confirm the wound healing efficacy of the fin thistle extract, the wound healing activity was confirmed after applying a wound to the fibroblasts.

Human fibroblasts were cultured in a 100 mm cell culture dish, and fibroblasts were wounded in the same manner as in <Experimental Example 5>. After culturing for 24 hours, the 2-well insert was removed with sterilized tweezers, and the fibroblast culture medium was replaced with a new culture medium. A basic culture medium was treated as a control group, and a culture medium supplemented with 20 ppm of fin thistle extract was treated as an experimental group for 24 hours.

As a result, as shown in Figures 6a and 6b, when the fin thistle extract was added, it was confirmed that the wound area was reduced and the migration of fibroblasts was increased. FIG. 6a is a photograph of a wound area compared with a microscope, and FIG. 6b is a result obtained by obtaining an area ratio using an Image J program (Java-based image processing program) and organizing the result into a graph. Through these results, it was confirmed that the fin thistle extract promotes the migration of fibroblasts and has higher wound healing activity than the control group.

< Experimental example 7> Measurement of skin volume and skin elasticity enhancement effect

The cream compositions of Example 1 and Comparative Examples 1 and 2 were prepared with the composition shown in Table 1 below. (Unit: % by weight)

ingredient name Example 1 Comparative Example 1 Comparative Example 2 fin thistle extract 1.0 - 20 glycerin 5.0 5.0 5.0 stearic acid 8.0 8.0 8.0 squalane 5.0 5.0 5.0 Self-emulsifying glycerin monostearate 2.5 2.5 2.5 Polyoxyethylene Sorbitan Monostearate 1.5 1.5 1.5 propylene glycol 4.0 4.0 4.0 Stearyl Glycyrrhetinate 0.2 0.2 0.2 vaseline 2.0 2.0 2.0 antioxidant Appropriate amount Appropriate amount Appropriate amount Spices Appropriate amount Appropriate amount Appropriate amount antiseptic Appropriate amount Appropriate amount Appropriate amount Purified water balance balance balance

A human application test was conducted on 30 women to investigate the effect of improving skin volume and skin elasticity when using the fin thistle extract of the present invention.

The creams prepared in Example 1 and Comparative Examples 1 and 2 were divided into 3 groups of 10 people and used twice a day, after washing in the morning and after washing in the evening, for 4 weeks.

In order to measure the skin volume enhancement effect after using the product, it was confirmed through facial skin sagging using a three-dimensional image. The side of the face was photographed using the PRIMOS® body (GFMesstechnik GmbH (Germany)), a three-dimensional facial imaging device, and the volume of the side of the face was analyzed using an image analysis device to quantify the degree of change. Table 2 below.

Improvement of skin volume (%) Example 1 8.9 Comparative Example 1 1.3 Comparative Example 2 0.9

Measure the elasticity of the face using a cutometer (Cutometer, Courage+Khazaka Electronic GmbH., Cologne, Germany), a device that measures skin elasticity by applying negative pressure to the skin to measure the effect of enhancing skin elasticity. and compared with before application, and the results are shown in Table 3 below.

Skin elasticity improvement (%) Example 1 48 Comparative Example 1 26 Comparative Example 2 28

From the results in Tables 2 and 3, it can be seen that when Example 1 including the fin thistle extract was used for 4 weeks, compared to Comparative Examples 1 and 2, the effect of enhancing skin volume and skin elasticity was excellent.

Hereinafter, based on the results of the above experimental examples, various formulation examples using fin thistle extract are formulated and presented below. However, these formulation examples are for explaining the present invention, and it is obvious to those skilled in the art that the formulation of the present invention is not limited to these formulation examples.

< formulation example 1> Soft lotion (skin toner)

ingredient name Content (% by weight) fin thistle extract 1.0 glycerin 5.0 1,3-Butylene glycol 3.0 ethanol 5.0 Polyoxyethylene nonylphenyl ether 0.5 Spices Appropriate amount antiseptic Appropriate amount Purified water balance

< formulation example 2> Astringent lotion ( astrigent )

ingredient name Content (% by weight) fin thistle extract 1.0 glycerin 3.0 citric acid 0.1 ethanol 10.0 Polyoxyethylene oleyl ether 1.0 sorbitol 2.0 Spices Appropriate amount antiseptic Appropriate amount Purified water balance

< formulation example 3> Lotion (emulsion)

ingredient name Content (% by weight) fin thistle extract 1.0 glycerin 5.0 1,3-Butylene glycol 8.0 squalane 10.0 Polyoxyethylene sorbitan monooleate 2.0 triethanolamine 1.5 Glyceryl Stearate 0.5 Stearyl Glycyrrhetinate 0.2 carboxyvinyl polymer 0.1 arginine 0.1 Spices Appropriate amount antiseptic Appropriate amount Purified water balance

< formulation example 4> cream

ingredient name Content (% by weight) fin thistle extract 1.0 glycerin 5.0 stearic acid 8.0 squalane 5.0 Self-Emulsifying Glycerin Monostearate 2.5 Polyoxyethylene Sorbitan Monostearate 1.5 propylene glycol 4.0 Stearyl Glycyrrhetinate 0.2 vaseline 2.0 antioxidant Appropriate amount Spices Appropriate amount antiseptic Appropriate amount Purified water balance

< formulation example 5> ointment

ingredient name Content (% by weight) fin thistle extract 1.0 Cetostearyl Alcohol 2.0 Self-emulsifying monostearic acid 2.0 stearic acid 1.0 beeswax 4.0 squalane 7.0 monostearin glycerin 3.0 Sorbitan Monostearate 1.0 Polysorbate 80 3.0 glycerin 5.0 propylene glycol 4.0 Spices Appropriate amount Vaseline balance

In the present invention, as shown in Tables 4 to 8, the composition for external application for skin prepared in various formulations using the extract of fin thistle exhibits excellent effects in promoting skin volume, skin elasticity, wrinkle improvement, anti-aging, skin regeneration and skin wound healing. can

Claims (9)

Contains fin thistle extract as an active ingredient,
The fin thistle extract promotes the differentiation of adipose-derived stem cells into fat cells.
According to claim 1,
The fin thistle extract is a composition for external application for skin for enhancing skin volume or enhancing skin elasticity, characterized in that it is included in 0.0001 to 10% by weight based on the total weight of the composition.
According to claim 1,
The fin thistle extract promotes the proliferation of adipose-derived stem cells.
delete According to claim 1,
The fin thistle extract increases the expression level of differentiation cluster 44 (CD44) and differentiation cluster 73 (CD73), which are adipose-derived stem cell markers.
According to claim 1,
The composition for external application for skin is a composition for external application for skin for improving skin volume or enhancing skin elasticity, characterized in that it is a composition for improving wrinkles, anti-aging, skin regeneration or wound healing.
According to claim 1,
The enhancement of skin volume or skin elasticity is a composition for external application for skin for enhancing skin volume or skin elasticity, characterized in that by increasing fat cells and adipose tissue.
A cosmetic composition comprising the fin thistle extract of any one of claims 1 to 3 and 5.
A pharmaceutical composition for treating skin wounds comprising the fin thistle extract of any one of claims 1 to 3 and 5.

Images