KR102490408B1 - Health food composition for strengthening immunity customized for individual and disease prevention and slug breeding method for the same - Google Patents

Abstract

The present invention measures the effect of the natural products we ingest on immunity enhancement for each individual, selects natural products effective for enhancing immunity for each individual, manufactures the selected natural products as slug feed, and uses this feed to grow slugs It relates to a health food composition for personalized immunity enhancement using slugs. Natural product search step of searching for a customized immunity enhancing natural product: b) After sterilizing the searched natural product under high pressure for 15 minutes and cooling it, lactic acid bacteria were added in an amount of 5 parts by weight based on the weight of the high-pressure sterilized product and fermented at 37 ° C for 7 days. A slug feed preparation step of preparing a slug feed by suppressing post-fermentation by steam sterilizing the solid fermented product at 100 ° C. for 30 minutes; and c) breeding slugs by using the prepared feed.

Description

Health food composition for strengthening immunity and preventing disease customized for each individual and method for breeding slugs for the same {Health food composition for strengthening immunity customized for individual and disease prevention and slug breeding method for the same}

The present invention measures the effect of the natural products we ingest on immunity enhancement for each individual, selects natural products effective for enhancing immunity for each individual, manufactures the selected natural products as slug feed, and slugs raised using this feed It relates to a health food composition for personalized immunity enhancement using.

The immune system can be divided into natural resistance, non-specific immune system and specific immune system. Natural resistance (first line of defense) refers to the anatomical and physiological factors that block all invaders, including microorganisms, regardless of their type, and non-specific immunity (second line of defense) is phagocytes that break through natural resistance and eliminate invaders entering the body. The bispecific immune system is a highly developed immune system with memory and the ability to distinguish between self and nonself.

Leukocytes form the 2nd or 3rd line of defense and break through the 1st line of defense to take charge of foreign substances that enter the body. In the event of bacterial or viral infection or inflammatory response, the regulation of macrophage and lymphocyte activity plays a role in determining the therapeutic effect of medicines. plays a pivotal role in

In addition, macrophages are cells with various functions and play an important role in the immune system by producing various cytokines and oxygen monoxide (NO) under oxidative stress.

The macrophages are antigen-presenting, tumoricidal, or microbicidal cells that play a central role in cell-mediated or humoral immunity. NO produced from activated macrophages exhibits phagocytosis, a non-specific host defense mechanism, and inhibits proliferation of bacteria and cancer cells. In particular, iNOS, which is expressed by stimuli such as lipopolysaccharide (LPS), cytokines, and TNF-α in macrophages, is known to promote NFκB activity by producing a large amount of NO for a long time. Reactive oxygen species (ROS) such as superoxide anion (O ₂ -), hydrogen peroxide (H ₂ O ₂ ) and nitric oxide (NO) by activated macrophages production is an important cytotoxic and cytostatic mechanism in non-specific immunity.

Regarding immune activity, in cancer patients, hematopoietic stem cells are damaged during anticancer treatment such as radiation therapy or chemotherapy, resulting in a decrease in hematopoietic cells and immune cells, which often causes disorders in hematopoiesis and immune function formation. Recently, it has been reported that not only the decrease in immune activity of cancer patients due to anticancer treatment, but also the weakening of immunity due to environmental reasons or lifestyle in general people without specific diseases.

Due to these circumstances, many plant extracts are currently used as health functional foods or medicines to improve health through immunity enhancement worldwide.

In the case of plant extracts capable of enhancing such immunity, it is possible to treat, prevent or improve diseases caused by bacterial or viral infections or cancer cells, and these effects are more effective in the body's immune function than by antibacterial or antiviral actions. Since it is by improvement, there is an advantage in being free from problems such as side effects.

Therefore, a composition containing an immune-enhancing component with high immunity-enhancing effect and weak side effects by selecting a substance having an immune-enhancing effect among natural products expected to relatively minimize the possibility of safety problems. development is required.

On the other hand, white-spotted flower radish, one of the edible insects, belongs to the order Coleoptera, chaferidae, and flower radish, called "manufacturing" or "sluggish" in traditional oriental medicine books such as private and donguibogam, and has a body length of 17 to 22 mm. , a herbivorous insect with a width of 12-1 mm, the body is dark copper and glossy, with scattered yellowish-white patterns. It inhabits Korea, China, Japan, and eastern Siberia, and is known to occur once every 1 to 2 years from April to October (Gocheon [古川], etc., encyclopedia of primary colored insects, Jipyangsa [集英社], Tokyo [Tokyo], 573-574 (1977); Zhang Jiyi, Chinese Economic Insect Journal 28, Chosimok, Chinese Zoology Compilation Committee, Chinese Academy of Sciences, Science Publishing House (1984)). The larvae of the white-spotted flower are classified into 1st to 3rd instars according to the size, and the average head width of the 3rd instar larvae is around 4.3~4.5㎜ and the body length is 25~37㎜. The body of the larvae at this stage is milky white and densely covered with short yellow hairs. Compared to the size of the body, the size of the head is small and the legs are not developed, so it moves using its back when moving.

White-spotted radish has long been used as an herbal medicine for the treatment of liver diseases and the like. In addition, it has recently been attracting great attention as an insect resource for the exploration and development of useful bioactive substances, production of antibiotic substances, restoration of liver lipid metabolism damaged by excessive alcohol intake in experiments using mice, etc. This has been known and its usefulness has been confirmed, such as studies on thrombolytic enzymes and showing hepatoprotective effects against hepatotoxicity induced by administration of carbon tetrachloride in rats.

In addition, through Patent Application No. 10-2017-0031760, production of feed for breeding white-spotted daisies using waste medium after cultivation of maitake mushrooms reduces costs and at the same time, anticancer and immune active ingredients unique to maitake mushrooms are produced. It is disclosed that a converted white-spotted flower can be obtained,

1. Korean Patent Application No. 10-2017-0031760

However, the health food for enhancing immunity using the larvae of the white-spotted daisies (hereinafter referred to as "sluggish") as described above is meaningful in that it can respond to cancer, viral diseases, and infectious diseases by improving immunity universally, but each person has individual Although it has the effect of enhancing the immunity of a specific person due to differences in temperament or environment, it can be found in many experiences that it may not be helpful at all.

In order to solve this problem, after a long period of research, we measure the effect of the natural products we consume on individual immunity enhancement, select natural products that are effective in improving immunity for each individual, and manufacture these selected food groups as slug feed. Thus, when slugs raised using this feed are used, it is possible to manufacture personalized immune enhancing health food using slugs, leading to the present invention.

Therefore, the technical problem to be achieved by the present invention is to provide a method for breeding slugs for manufacturing personalized immunity-enhancing health food and a health food composition for enhancing immunity using slugs produced by the breeding method.

In order to achieve the above technical problem, the present invention

a) Natural product search step of searching for a personalized immunity-enhancing natural product by comparing the change in immunity when there is no natural product material and when the natural product material is added using the individual's blood and natural product material:

b) After sterilizing the searched natural product under high pressure for 15 minutes and cooling it, lactic acid bacteria were added in an amount of 5 parts by weight relative to the weight of the high-pressure sterilized material, and the solid fermentation product obtained by fermentation at 37 ° C for 7 days was steam sterilized again at 100 ° C for 30 minutes. A slug feed preparation step of preparing a slug feed by processing and suppressing post-fermentation; and

c) breeding slugs using the prepared feed

It provides a slug breeding method for manufacturing a personalized immune enhancing health food, characterized in that it comprises.

In the breeding method according to the present invention as described above, it is preferable that the lactic acid bacteria are Lactobacillus Casei .

In the breeding method according to the present invention as described above, it is preferable to add minced ginger and licorice powder to the natural product sterilized after high-pressure sterilization in step b), stir-fry, and then cool to add lactic acid bacteria.

In the breeding method according to the present invention as described above, it is preferable to further add illite powder in the same amount of weight when adding lactic acid bacteria in step b).

In the breeding method according to the present invention as described above, step (c) is preferably a method in which the feed prepared in step (b) is mixed with sawdust feed for slugs and fed to slugs.

In the breeding method according to the present invention as described above, it is preferable to include the feed prepared in step b) in the weight ratio of 1/25 to the sawdust feed for slugs.

In the breeding method according to the present invention as described above, in the step (c), the feed prepared in step (b) is fed to the slugs while stopping the supply of slug sawdust feed 7 to 14 days before the slugs are shipped. it is desirable

In order to achieve another technical problem, the present invention provides a personalized health food composition for enhancing immunity, characterized in that it comprises the slugs according to any one of the above items.

In the health food composition according to the present invention, the slugs may be included in the form of dry powder or extract.

According to the present invention, the effect of the natural products we consume on the immunity enhancement of each individual is measured, and natural products effective in enhancing immunity are selected for each individual, and the selected natural products are prepared as slug feed, and the feed is used to raise In the case of using slugs, it is possible to manufacture a personalized health food for enhancing immunity using slugs.

1 is a graph showing the lymphocyte proliferation activity by the composition according to the present invention.
Figure 2 is a graph showing the induction ability of the cytokine IL-2 of the composition according to the present invention.
Figure 3 is a graph showing the induction ability of the cytokine IL-4 of the composition according to the invention.
Figure 4 is a graph showing the induction ability of the cytokine INF-γ of the composition according to the present invention.

Hereinafter, it will be described in detail and in detail so that the present invention can be easily practiced.

the present invention

a) Natural product search step of searching for a personalized immunity-enhancing natural product by comparing the change in immunity when there is no natural product material and when the natural product material is added using the individual's blood and natural product material:

b) After sterilizing the searched natural product under high pressure for 15 minutes and cooling it, lactic acid bacteria were added in an amount of 5 parts by weight relative to the weight of the high-pressure sterilized material, and the solid fermentation product obtained by fermentation at 37 ° C for 7 days was steam sterilized again at 100 ° C for 30 minutes. A slug feed preparation step of preparing a slug feed by processing and suppressing post-fermentation; and

c) breeding slugs using the prepared feed

Including natural products we ingest, we measure the effect on immunity enhancement for each individual, select natural products that are effective in enhancing immunity for each individual, manufacture the selected natural products as slug feed, and feed slugs raised using this feed. When used, it is intended to provide a health food for enhancing immunity customized for each individual using slugs.

First, the steps of searching for natural products for individual immunity enhancement will be described.

This natural product search step can be used without particular limitation as long as it is widely known in the art to which the present invention belongs, and examples thereof include Korean Patent Publication No. 10-2017-0110297, Patent Publication No. 10-2011-0010030 and Patent Publication No. It is described in No. 10-2011-0084118, and this content is naturally included in the present invention. It is not limited to this.

Looking specifically at the contents disclosed in Korean Patent Publication No. 10-2017-0110297 in the above example note

a) drawing blood;

b) injecting and culturing antigen cells into the blood;

c) analyzing the cultured blood to measure the death of antigen cells and the concentration of cytokines including IFN-γ;

d) culturing by adding antigen cells and natural materials to the blood of step a);

e) analyzing the blood cultured in step d) to measure the death of antigen cells and the concentration of cytokines including IFN-γ; and

f) comparing and analyzing the measurement result of step c) and the measurement result of step e).

In the above method, when the blood is the blood of a sick person, antigen cells are not added in steps b) and d), and the antigen cells may be floating cells.

In addition, in the above method, immunity enhancement can be tested by examining the activity enhancement of natural killer cells (NK cells), natural killer T cells (NKT cells), and innate lymphoid cells.

in more detail

1) The step of examining the individual's immune ability

1-1) Blood sampling step

[Take the blood of a healthy person or a sick person. Here, the sick include cancer patients or people infected with viruses or the like.

1-2) Culturing by adding antigen cells to blood

Antigen cells are added to the blood of healthy people and cultured, and the blood of sick people is cultured to suit the characteristics of the antigen cells. Here, it is conditional that the antigen cells to be injected or the antigen cells of the patient are floating cells. The reason is that adherent cells cannot be used because NK cells are floating cells.

For example, the antigen cells to be added may be human cancer cells, preferably K562 cells. K562 cells are a cell line derived from a patient with chronic myeloid leukemia, and they are originally Philaderphia chromosome positive (Ph+), bcr-Abl positive cells. In addition to being used in stem cell research because it shows properties similar to pluripotent stem cells that differentiate into red blood cells, granulocytes, monocytes, etc. depending on stimulation, it is highly sensitive to cytotoxicity by NK cells, so it can be used for measuring NK cell activity. can

In addition, DMEM and RPMI media containing 10% FBS and 1% penicilin and streptomycin are used for human cancer cell culture. The cell number was measured at 1×10 ⁸ cell/mL using a hemocytometer, and incubated in T25 cell culture flasks for 24 hours at 37° C., 5%, CO ₂ incubator. Subculture is carried out once every 2-3 days. However, culture conditions or culture period may be changed depending on the characteristics of antigen cells.

1-3) Apoptotic ability and cytokine measurement step

The cultured blood is analyzed to measure the death of antigen cells and cytokines including IFN-γ. Apoptosis analysis of antigenic cells may be performed by measuring cell proliferation rate using MTT assay.

MTT (3-[4,5-dimethyl thiazol-2-yl]-2,5-yl tetrazolium bromide) assay is a method for measuring cell toxicity and viability. It uses the principle of producing water-insoluble purple formazan. To this end, 200 μL of each cell line was added to 96 wells at a concentration of 1×10 ⁴ cells/well for 24 hours at 37° C., and cultured in a 5% CO ₂ incubator for stabilization. The prepared extract is treated in a medium at an appropriate concentration and cultured for 48 hours. The medium was treated with MTT (3-[4,5-dimethyl thiazol-2-yl] -2,5-yl tetrazolium bromide) assay reagent and reacted for 4 hours in a 37℃, 5% CO ₂ incubator, followed by MTT reaction solution Remove

200μL of DMSO was treated to dissolve the generated formazan, and absorbance was measured at 540nm with an ELISA spectrophotometer.

In addition, concentration changes of cytokines including IFN-γ can be performed by ELISA technique. Dilute purified anti-human cytokines (IFN-γ) monoclonal antibody at 2 μg/mL in binding buffer (0.1 M sodium phosphate buffer, pH 9.0) on a 96-well plate, add 50 μL/well, and leave overnight at 4°C. do. After washing with PBS/Tween, blocking buffer (1% BSA/PBS) was added at 200 μL/well, and then left at 37° C. for 2 hours. After washing, add 100 μL of the supernatant collected by treating the standard cytokine and extract, and leave overnight at 4℃. After washing with PBS/Tween, the biotinylated anti-cytokine detection antibody is diluted in blocking buffer/Tween, treated with 100 μL/well, and left at 37° C. for 1 hour. After washing again with PBS/Tween, 1/1000 diluted streptavidin-HRP was added at 100 μg/well in blocking buffer/Tween, left for 1 hour, and then washed. Add 100 μg/well of TMB solution and after 15 minutes, add 50 μL/well of 1 M HCl as a stop reagent and measure the absorbance at 450 nm with an ELISA spectrophotometer.

Depending on the design conditions, other methods may be used for the detection of antigen cell death and cytokines including IFN-γ.

1-4) Immunity test step

The death of antigen cells is analyzed, and the difference between the concentration of cytokines analyzed in the blood of a normal person and the concentration of cytokines after injection of antigen cells is analyzed.

After that, the immune ability according to the death of antigen cells and the change in cytokine concentration can be scored by using the numerical value presented in the medical database or by preparing a separate standard condition.

2) Step of examining immunity enhancement of natural materials

2-1) Blood sampling step

Perform the same as step 1-1) above.

The step of injecting and culturing antigen cells and natural materials into blood

It is performed in the same manner as in step 1-2) above, but a natural material is additionally added to the blood.

Here, the component of the natural material may be a herbal preparation, a food preparation, or a main ingredient of food. Natural materials may be extracts or concentrates extracted using hot water, ethanol, fermentation or fractionation in the case of herbal preparations, and may be samples obtained by pulverizing, powdering, or dissolving food in the case of food preparations or major ingredients of food.

2-3) Apoptotic ability and cytokine measurement step

Perform the same as steps 1-3) above. 2-4) Test for immunity enhancement

It is performed in the same manner as in steps 1-4) above, but the immune enhancing ability of natural materials is examined by comparing and analyzing the results of steps 1-4). That is, if the death of antigen cells and the secretion of cytokines including IFN-γ were increased compared to the results analyzed in steps 1-4), it can be seen that the immune enhancing ability of the natural material to be tested was confirmed.

After that, the optimal concentration may be analyzed by repeating steps 2-1) to 2-4) according to the concentration of the same natural material. In addition, by performing steps 2-1) to 2-4) under the same conditions for a plurality of natural material materials, it is possible to analyze the optimal natural material material.

Next, a method for preparing slug feed using the selected natural product will be described.

In the present invention, the selected natural product is sterilized under high pressure, cooled, and then 5 parts by weight of lactic acid bacteria is added and fermentation is performed at a temperature of 36° C. for 7 days.

At this time, there is no need to supply moisture, and since the moisture required for microbial fermentation is sufficient for the moisture absorbed by the material itself during the high-pressure sterilization process, a separate supply is not required.

The most preferred lactic acid bacteria for obtaining the fermented feed of the present invention was Lactobacillus Casei .

The solid fermented feed material of the present invention fermented by the fermentation strain of the present invention is sterilized by steam heat treatment at 100 ° C. for 30 minutes.

It was confirmed that the slug feed prepared according to the present invention has no problem in breeding slugs, and the immunity enhancing substances contained in the feed are more easily accumulated by the slugs.

In addition, in order to further enhance the above-mentioned effect, in the present invention, the selected natural product is sterilized under high pressure, then minced ginger and licorice powder are put in a heated pan, roasted, and then cooled to inoculate lactic acid bacteria.

The minced ginger and licorice powder are the same weight ratio, and their total content is preferably 10 parts by weight relative to the weight of the natural product.

In addition, when adding lactic acid bacteria, it is very advantageous to increase the efficiency of lactic acid bacteria fermentation and slug breeding by adding the same weight of illite powder as the weight of the lactic acid bacteria.

Illite used in the present invention was first discovered in Illinois, USA in 937, and is a rare mineral that has the largest reserve in Yeongdong-gun, North Chungcheong Province worldwide, and the components of illite are silicon dioxide (SiO ₂ ), alumina ( Ai ₂ O ₃ ), iron oxide (Fe ₂ O ₃ ), calcium oxide (CaO), sodium oxide (Na ₂ O), magnesium oxide (MgO), TiO ₂ , MnO and K ₂ O, etc. It has effects such as sebum removal, blood circulation promotion, collagen binding, detoxification, and moisture control.

Next, a method for breeding slugs using the prepared feed will be described.

The slug breeding method in the present invention can be divided into two types. The first method is to mix and supply the feed according to the present invention with the known sawdust feed used for slug breeding throughout the slug breeding period.

At this time, it was found that the supply amount of the feed was optimal for various tests of the present inventors to put about 1/25 of the known sawdust feed and weight.

Another method is to feed the slugs while stopping supply of sawdust feed to the slugs before manufacturing healthy food, that is, 7 to 14 days before shipment. In this case, the slugs are moved to a breeding container without sawdust and only the feed prepared according to the present invention is fed to the slugs. At this time, it is preferably fed for about 10 days. Active substances accumulate more readily, which is highly desirable.

It will be described in detail through examples and experimental examples below.

< Example 1>

The blood of a man in his 50s was collected and tested on king oyster mushrooms, olives, kelp, water parsley, grapes, apples, broccoli, and spinach by the method disclosed in Korean Patent Publication No. 10-2017-0110297, king oyster mushrooms, olives , Water parsley, and broccoli were confirmed to enhance immunity, and other foods did not lower immunity, but the enhancing effect was not confirmed.

The natural products were divided into an immunity enhancing group and a control group, and king oyster mushrooms, olives, water parsley, and broccoli were used in the immune enhancing group, and kelp, grapes, apples, and spinach were used in the comparative group, and these were prepared as slug feed.

The feed was sterilized by high-pressure sterilization, cooling, adding 5 parts by weight of Lactobacillus Casei , fermenting at a temperature of 36 ° C for 7 days, and then steam heating at 100 ° C for 30 minutes to sterilize.

. < Example 2>

After high-pressure sterilization of king oyster mushrooms, olives, water parsley, and broccoli, which improved immunity in Example 1, ginger and licorice powder minced without oil in a well-heated pan were added in equal weight to 100 parts by weight of natural products sterilized under high pressure. 10 parts by weight added It is the same as in Example 1 except that it was roasted and then cooled and used.

. < Example 3>

It is the same as in Example 2, but there is a difference in that more illite powder is added in the same weight ratio as the lactic acid bacteria.

<Breeding method>

Subsequently, 5 breeding tubes were prepared, the first test group 1 eating the immunity enhancing group feed of Example 1, the second test group 2 eating the immunity enhancing group feed of Example 2, and the third being Example 3 The test group 3 consumed the immunity boosting group feed, the fourth control group 1 consumed the control group feed, and the fifth was the control group 1 who consumed the normal sawdust feed. The weight was also selected to be the same, and it was divided and bred in each breeding tube 10 days before shipment.

Before manufacturing health food, there was no significant difference in the weight of slugs depending on the breeding method, and the slugs were prepared by fasting for two days and then freeze-drying.

< test example 1: in the blood before and after administration lymphocyte number change>

The freeze-dried slugs were administered to testers who donated blood, and changes in lymphocytes (unit: %) in the blood before and after administration were measured and shown in Table 1 below. The dose was 2000 mg once daily.

before administration 5 days after dosing 10 days after administration normal range test group 1 28 36 40
35-41% test group 2 28 38 40 test group 3 28 40 40 comparator group 1 28 31 34 Control 1 28 31 34

Looking at Table 1, in the test group of the present invention, was the immunity slightly lowered before administration? It was confirmed that it was increased compared to before Myitkyina administration.

In particular, in test group 2 and test group 3, the results were found to be higher in the normal range after 5 days of administration, and it was confirmed that the effect was very remarkable.

< test example 2: Example 1 in- using extract virto test>

The following tests were conducted using the extract prepared by adding 1,000 mL of ethyl alcohol to the slugs of Example 1, extracting twice for 24 hours in a 25 ° C water bath, and filtering with a vacuum filter.

<Lymphocyte proliferation Experiments on activity >

The spleen was removed from a 5-week-old Balb/c mouse, and the number of splenocytes was adjusted to a density of 2×10 ⁵ /100 μl, put into each well, and the composition according to the present invention was added to each well at 200 μg/ml. , 100 μg/ml, 50 μg/ml and 10 μg/ml were added.

As a positive control, the final concentrations of Concavanallin-A (hereinafter abbreviated as Con-A) and Lipopolysaccharide (hereinafter abbreviated as LPS), which are mitotic substances of T-cells and B-cells, were respectively It was adjusted to 0.5 μg/ml and 5 μg/ml and treated in each well, and the experimental group and the control group were cultured for 3 days at 37° C. and 5% CO ₂ conditions, and the proliferation assay of lymphocytes by the sample was investigated.

The proliferation activity of lymphocytes was performed by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] method, and the MTT method was performed at a concentration of 5 mg/ml. After adding 50 μl of MTT reagent adjusted to each well and incubating for 6 hours, the reaction between MTT reagent and mitochondrial dehydrogenase produced by living cells resulted in the formation of water-insoluble dark blue formazan. The proliferative activity of lymphocytes was measured by measuring the absorbance at 570 nm.

As shown in FIG. 1, the results of this experimental example showed that the composition according to the present invention exhibited higher proliferation activity of splenocytes than the sample-untreated control group.

As a result, it was not confirmed which type of immune cell, B-cell or T-cell, was activated by the present invention, but it was confirmed that there was an active action of directly stimulating spleen cells in a non-specific way.

< cytokines experiments on induction >

The spleen was removed from a 5-week-old Balb/c mouse, and the number of splenocytes was adjusted to a density of 2×10 ⁶ /500 μl, put into each well, and 200 μg/ml of the composition of the present invention, respectively. , 100 μg/ml, 50 μg/ml and 10 μg/ml were added and co-cultured for 24 hours.

In addition, as a positive control, the final concentration of Con A was adjusted to 0.5 μg/ml, treated in wells, and cultured for 24 hours. After completion of the culture, only the supernatant was recovered and induced secretion of IL-2 and IL- Various cytokines such as 4, IL-10, and INF-γ were measured using each cytokine kit.

As shown in FIGS. 2 to 4, the results of this test directly activate the pancreatic cells of the mouse to induce immune-related cytokines such as IL-2, IL-4, IL-10, and INF-γ. confirmed.

In the present invention, the degree of cytokine induction was different depending on the type of cytokine and the concentration of the composition, but it was confirmed that the activity as a cytokine inducer was dependent on the concentration.

< Example 4>

The blood of a man in his 60s who likes meat was taken and tested for beef, pork, chicken, duck, lamb, green beans, mackerel, and eggs by the method disclosed in Korean Patent Publication No. 10-2017-0110297. As a result, immunity enhancement was confirmed in chicken, green bean, and mackerel, and immunity was not lowered in other foods, but the enhancing effect was not confirmed.

The natural product was divided into an immunity enhancing group and a control group, and chicken, mouse eye pea, and mackerel were used in the immune enhancing group, and beef, pork, duck, and lamb were used in the comparative group, and these were prepared as slug feed.

The feed was sterilized by high-pressure sterilization, cooling, adding 5 parts by weight of Lactobacillus Casei , fermenting at a temperature of 36 ° C for 7 days, and then steam heating at 100 ° C for 30 minutes to sterilize.

. < Example 5>

After high-pressure sterilization of chicken, mouse's eye, and mackerel, which improved immunity in Example 4, equal weight of minced ginger and licorice powder without oil in a well-heated pan. 10 parts by weight compared to 100 parts by weight of high-pressure sterilized natural product. Roasted It is the same as Example 4 except that it was used after cooling.

. < Example 6>

It is the same as Example 5, but there is a difference in that more illite powder is added in the same weight ratio as the lactic acid bacteria.

<Breeding method>

Subsequently, five breeding tubes were prepared, the first test group 4 eating the immunity enhancing group feed of Example 4, the second test group 5 eating the immunity enhancing group feed of Example 5, and the third being Example 6 The test group 6 consumed an immunity boosting group feed, the fourth control group 2 consumed a control group feed, and the fifth was a control group 2 that consumed a regular sawdust feed. The weight was also selected to be the same, and it was divided and bred in each breeding tube 10 days before shipment.

Before manufacturing health food, there was no significant difference in the weight of slugs depending on the breeding method, and the slugs were prepared by fasting for two days and then freeze-drying.

< test example 3: in the blood before and after administration lymphocyte number change>

The lyophilized slugs were administered to testers who donated blood, and changes in lymphocytes (unit: %) in the blood before and after administration were measured and shown in Table 2 below. The dose was 2000 mg once daily.

before administration 5 days after dosing 10 days after administration normal range test group 4 26 36 40
35-41% test group 5 26 38 40 test group 6 26 40 41 comparison group 2 26 30 34 Control 2 26 29 32

Referring to Table 2, in the test group of the present invention, although the immunity was slightly lower before administration, it was within the normal range after administration on the 5th day, and after administration on the 10th day, it was located at the top of the normal range, but in the control group and the control group, it was slightly below the normal range. It was confirmed that it was increased compared to before Myitkyina administration.

In particular, in test group 5 and test group 6, the results were found to be at the top even in the normal range after 5 days of administration, confirming that the effect was very remarkable.

As described above, according to the present invention, the effect of the natural products we ingest on the immunity enhancement of each individual is measured, and natural products effective for enhancing immunity are selected for each individual, and the selected natural products are prepared as slug feed to obtain this feed In the case of using slugs bred using slugs, it is possible to manufacture a personalized immune enhancing health food using slugs.

Claims (9)

a) Natural product search step of searching for a personalized immunity-enhancing natural product by comparing the change in immunity when there is no natural product material and when the natural product material is added using the individual's blood and natural product material:
b) After sterilizing the searched natural product under high pressure for 15 minutes and cooling it, lactic acid bacteria were added in an amount of 5 parts by weight relative to the weight of the high-pressure sterilized material, and the solid fermentation product obtained by fermentation at 37 ° C for 7 days was steam sterilized again at 100 ° C for 30 minutes. A slug feed preparation step of preparing a slug feed by processing and suppressing post-fermentation; and
c) breeding slugs using the prepared feed
include,
After high-pressure sterilization in step b), minced ginger and licorice powder are added to the sterilized natural product, roasted, cooled, and lactic acid bacteria are added,
A slug breeding method for producing a personalized immune-enhancing health food, characterized in that when adding the lactic acid bacteria in step b), the illite powder is further added in the same weight.
According to claim 1,
Slug breeding method for producing a personalized immune enhancing health food, characterized in that the lactic acid bacteria is Lactobacillus Casei .
According to claim 1 or 2,
Wherein step (c) is a method of mixing the feed prepared in step (b) with sawdust feed for slugs and ingesting it to slugs.
According to claim 3,
A slug breeding method for producing a personalized immune-enhancing health food, characterized in that the sawdust feed for slugs contains 1/25 of the weight ratio of the feed prepared in step b).
According to claim 1 or 2,
The step (c) is a method of ingesting the feed prepared in step (b) to the slugs while stopping the supply of sawdust feed to the slugs 7 to 14 days before the slugs are shipped. How to breed slugs for
A health food composition for personalized immunity enhancement, characterized in that it comprises slugs raised by the breeding method according to claim 1 or 2.
[Claim 7] The health food composition for personalized immunity enhancement according to claim 6, wherein the slugs are included in the form of dry powder or extract.


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