KR102469249B1 - Functional cosmetic and manufacturing method for the same - Google Patents

Abstract

The present invention relates to a manufacturing method of functional cosmetics which comprises the steps of: preparing a solution containing porous PLLA nanoparticles; coating the inside and outside of the porous PLLA nanoparticles with a culture material derived from stem cells by mixing the solution and a culture medium containing the culture material; and coating the outside of the porous PLLA nanoparticles with hyaluronic acid by mixing the solution and a hyaluronic acid solution in which TiO_2 is dissolved. The cosmetics according to the present application further includes a component such as PLLA in addition to a stem cell-derived component, and thus has excellent skin tissue regeneration performance compared to cosmetics simply containing either the stem cell-derived component or PLLA.

Description

Functional cosmetics and their manufacturing method {FUNCTIONAL COSMETIC AND MANUFACTURING METHOD FOR THE SAME}

The present application relates to cosmetics and methods for their preparation.

Stem cells are undifferentiated cells, which can divide and self-renew for a long period of time, and refer to cells that can differentiate into various types of cells when certain conditions are given. Stem cells are classified into embryonic stem cells and adult stem cells depending on the tissue of origin. Although their potential has a limited disadvantage compared to embryonic stem cells, many treatments are being studied for adult stem cells that have no ethical problems and no side effects. .

When stem cells are cultured, the stem cells secrete various substances into the culture medium. This culture has been shown to have significant biochemical activity in several studies. Recently, attempts to use a culture medium in which effective substances are maximized by culturing stem cells in this way as a cosmetic material are increasing.

Korean Patent Registration No. 10-13364748, which is the background of the present application, relates to a cosmetic composition comprising nanofibers or nanocapsules of adult stem cell culture medium derived from quantum fat and a method for manufacturing the same.

The present application is to solve the problems of the prior art described above, and provides cosmetics and a manufacturing method thereof.

However, the technical problem to be achieved by the embodiments of the present application is not limited to the technical problems described above, and other technical problems may exist.

As a technical means for achieving the above technical problem, the first aspect of the present application relates to a method for manufacturing cosmetics, preparing a solution containing porous PLLA nanoparticles, a culture material derived from the solution and stem cells. Coating the inside and outside of the porous PLLA nanoparticles with the culture material by mixing a culture medium containing a mixture, and mixing the solution and a hyaluronic acid solution in which TiO2 is dissolved to coat the outside of the porous PLLA nanoparticles with hyaluronic acid. It provides a method for manufacturing cosmetics, including the step of coating.

According to one embodiment of the present application, the diameter of the porous PLLA nanoparticles may be 50 nm to 200 nm, but is not limited thereto.

According to one embodiment of the present application, the diameter of the pores of the porous PLLA nanoparticles may be 10 nm to 60 nm, but is not limited thereto.

According to one embodiment of the present application, the culture medium and the hyaluronic acid solution are each independently vitamin C, tifenoid, mucin, allatoin, glycolic acid, collagen, elastin, epidermal growth factor (EGF) and these It may further include one selected from the group consisting of combinations of, but is not limited thereto.

According to one embodiment of the present application, mixing the solution and the culture medium may be performed in an environment having a pH of 7 to 8, but is not limited thereto.

According to one embodiment of the present application, the step of mixing the solution and the hyaluronic acid solution may be performed in an environment in which a voltage of 10 kV is applied, but is not limited thereto.

According to one embodiment of the present application, after coating the porous PLLA nanoparticles with hyaluronic acid, heat treatment at 50°C and heat treatment at 60°C may be further included, but is not limited thereto.

According to one embodiment of the present application, the culture material may include exosomes, but is not limited thereto.

Also, the second aspect of the present application relates to a cosmetic, prepared according to the first aspect.

The above-described problem solving means are merely exemplary and should not be construed as intended to limit the present disclosure. In addition to the exemplary embodiments described above, additional embodiments may exist in the drawings and detailed description of the invention.

The cosmetic according to the present application, in addition to the stem cell-derived component, further includes a component such as PLLA, and thus has excellent skin tissue regeneration performance compared to a cosmetic product simply containing either a stem cell-derived component or PLLA.

In addition, since the cosmetics include exosomes, hyaluronic acid, and cellulose nanofibers, skin regeneration performance is excellent, and drying of the cosmetics applied to the skin in the air can be minimized, so the effect of the cosmetics is maintained for a long time. .

In addition, since the cosmetic according to the present application contains TiO ₂ , it has excellent sunscreen effect, and TiO ₂ can be fixed to the skin surface by cellulose nanofibers and hyaluronic acid. That is, even if the skin on which the cosmetic is applied sweats, TiO ₂ may not flow down due to sweat.

However, the effects obtainable herein are not limited to the effects described above, and other effects may exist.

1 is a flow chart of a method for manufacturing a cosmetic comprising a stem cell component according to one embodiment of the present application.

Hereinafter, embodiments of the present application will be described in detail so that those skilled in the art can easily practice with reference to the accompanying drawings. However, the present disclosure may be implemented in many different forms and is not limited to the embodiments described herein. And in order to clearly describe the present application in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification.

Throughout this specification, when a part is said to be "connected" to another part, this includes not only the case of being "directly connected" but also the case of being "electrically connected" with another element in between. do.

Throughout the present specification, when a member is referred to as being “on,” “above,” “on top of,” “below,” “below,” or “below” another member, this means that a member is located in relation to another member. This includes not only the case of contact but also the case of another member between the two members.

Throughout the present specification, when a part "includes" a certain component, it means that it may further include other components without excluding other components unless otherwise stated.

As used herein, the terms "about," "substantially," and the like are used at or approximating that number when manufacturing and material tolerances inherent in the stated meaning are given, and are intended to assist in the understanding of this disclosure. Accurate or absolute figures are used to prevent undue exploitation by unscrupulous infringers of the stated disclosure. In addition, throughout the present specification, “steps of” or “steps of” do not mean “steps for”.

Throughout the present specification, the term "combination thereof" included in the expression of the Markush form means one or more mixtures or combinations selected from the group consisting of the components described in the expression of the Markush form, and the components It means including one or more selected from the group consisting of.

Throughout this specification, reference to "A and/or B" means "A, B, or A and B".

Hereinafter, the cosmetics of the present application and their manufacturing method will be described in detail with reference to embodiments and examples and drawings. However, the present application is not limited to these embodiments and examples and drawings.

As a technical means for achieving the above technical problem, the first aspect of the present application is to prepare a solution containing porous PLLA nanoparticles, mixing the solution and a culture medium containing a culture material derived from stem cells to Coating the inside and outside of porous PLLA nanoparticles with the culture material, and coating the outside of the porous PLLA nanoparticles with hyaluronic acid by mixing the solution and a hyaluronic acid solution in which TiO ₂ is dissolved, A manufacturing method for cosmetics is provided.

Cosmetics according to the present application may include ingredients derived from stem cell tissues, but are not limited thereto. In this regard, stem cell tissue itself has been banned from being used in cosmetics because it may contain ethical issues or human-borne infectious bacteria. After culturing the stem cell tissue in the medium, the culture medium in which the stem cell tissue is removed from the medium contains only substances generated in the process of culturing the stem cell tissue and does not contain the stem cell tissue itself, so it can be used in cosmetics. .

As will be described later, cosmetics according to the present application may mean a sunscreen such as a sunscreen.

First, a solution containing porous PLLA nanoparticles was prepared (S100).

According to one embodiment of the present application, the diameter of the porous PLLA nanoparticles may be 250 nm to 400 nm, but is not limited thereto. Preferably, the diameter of the porous PLLA nanoparticles may be 300 nm to 350 nm.

If the diameter of the porous PLLA nanoparticles is less than 250 nm, it is difficult to place the culture material therein, which will be described later, and if the diameter of the porous PLLA nanoparticles exceeds 400 nm, the porous PLLA nanoparticles coated with hyaluronic acid will be described later. Since the size is too large, the porous PLLA nanoparticles cannot permeate into the skin, and the efficacy of cosmetics may be reduced.

According to one embodiment of the present application, the diameter of the pores of the porous PLLA nanoparticles may be 10 nm to 60 nm, but is not limited thereto. Preferably, the diameter of the pores of the PLLA porous nanoparticles may be 20 nm to 50 nm, but is not limited thereto.

Specifically, when the diameter of the pore is less than 10 nm, exosomes cannot penetrate inside, and when the pore exceeds 60 nm, even if the culture material enters the inside of the porous PLLA nanoparticle, it can be easily escaped. .

In this regard, the pores exist on the surface of the porous PLLA nanoparticles, and as will be described later, the inside of the porous PLLA nanoparticles may be coated with a culture material and/or hyaluronic acid due to the pores.

PLLA according to the present application is a kind of biodegradable polymer, and unlike conventional fillers and Botox, it can promote the production of collagen and dermal cells while being harmless to the human body.

According to one embodiment of the present application, the porous PLLA nanoparticles are prepared by dissolving PLLA in acetone, then dropping the acetone into a PBS (Phosphate-buffered saline) solution, and heating the PBS solution to remove acetone. It may include the step of doing, but is not limited thereto.

The PBS solution is a solution capable of maintaining a constant pH at 7 to 8, and may be sterilized.

Specifically, when the acetone in which the PLLA is dissolved is dropped into the PBS solution and the PBS solution is heated, the acetone mixed in the PBS solution may be vaporized and removed due to a difference in boiling point.

According to one embodiment of the present application, when removing acetone from the PBS solution, air may be supplied into the PBS solution, but is not limited thereto.

When the PBS solution is heated while supplying air into the PBS solution, the solubility of the gas in the solution decreases and the supplied air may be discharged out of the PBS solution. At this time, it can be confirmed that a plurality of pores are formed in the PLLA nanoparticles while the air is discharged.

According to one embodiment of the present application, the air supply rate may be 10 cc/sec to 50 cc/sec, but is not limited thereto. At this time, the air supply rate is based on when the volume of the PBS solution is 150 ml (cc), and when the volume of the PBS solution changes, 1/15 (10 cc) to 1/3 (50 cc) is supplied per second means that

If the air supply rate is less than 10 cc/sec, the degree of formation of porous PLLA nanoparticles is rapidly reduced, and if it exceeds 50 cc/sec, the porous PLLA nanoparticles generated due to excessive air supply are damaged, and the number of PLLA nanoparticle structures is reduced. rather decreased.

According to one embodiment of the present application, the air may be supplied to the bottom of the PBS solution, but is not limited thereto.

When air is supplied to the PBS solution, when air is blown on the surface of the PBS solution, the air cannot be dissolved in the PBS solution and may escape from the solution by heat. However, when air is supplied after connecting a hose into the PBS solution, the air is dissolved in the PBS solution, and then released in the form of bubbles as the solubility is lowered by heat, and at this time, collides with PLLA nanoparticles during the release process. Thus, porous PLLA nanoparticles may be formed.

According to one embodiment of the present application, the PBS solution may further include poly(vinyl alcohol) (PVA), but is not limited thereto. The PVA is used to ensure that the PLLA is well dispersed in the PBS solution, and the size of the PLLA nanoparticles can be controlled.

The porous PLLA nanoparticles may be measured through a dynamic light scattering technique, but are not limited thereto.

According to one embodiment of the present application, the PBS solution may include cellulose nano fibers (CNF), but is not limited thereto.

The CNF is a nano- or micro-sized rod-shaped particle or fiber in which cellulose chains are bundled together, and is prepared by mixing the cellulose fibers with distilled water at a concentration of 1 wt% to 2 wt% to form a suspension and then introducing it into a high-pressure homogenizer. can At this time, when the concentration of the cellulose fibers in the suspension is less than 1 wt%, the degree of contact between the cellulose fibers is low when a high pressure is applied, resulting in a low yield of cellulose nanofibers, and when the concentration exceeds 2 wt%, the cellulose fibers are excessively contacted with each other, resulting in cellulose The yield of nanofibers is low.

According to one embodiment of the present application, the cellulose nanofibers may be prepared by a process comprising mixing cellulose fibers and water to form a suspension, and passing the suspension on a high pressure homogenizer. , but is not limited thereto.

At this time, the cellulose fibers may be in a crushed state, but are not limited thereto.

According to one embodiment of the present application, when passing the suspension on a high-pressure homogenizer, air may be continuously injected into the suspension, but is not limited thereto. The air is not discharged to the outside of the suspension in the high-pressure homogenizer, but can assist in crushing the cellulose fibers into cellulose nanofibers, and can be naturally discharged to the outside of the liquid when passing through the high-pressure homogenizer.

According to one embodiment of the present application, the amount of air injected into the suspension may be 1 cc/sec to 3 cc/sec, but is not limited thereto. When the air injection amount was less than 1 cc/sec, there was no significant difference from when no air was injected, and when the air injection amount exceeded 3 cc/sec, it was difficult to control the speed while the suspension passed through the high-pressure homogenizer.

In a solution containing the cellulose nanofibers and the porous PLLA nanoparticles, the porous PLLA nanoparticles may be coated with the cellulose nanoparticles, but is not limited thereto.

Specifically, before performing the step of coating the inside and outside of the porous PLLA nanoparticles with a culture material, which will be described later, by stirring the solution, the cellulose nanofibers can be attached to the surface or inside of the porous PLLA nanoparticles. At this time, if the temperature is raised, the cellulose nanofibers may be decomposed.

According to one embodiment of the present application, the suspension may be subjected to a pressure of 100 MPa to 200 MPa, but is not limited thereto. Preferably, the suspension may be subjected to a pressure of 150 MPa, but is not limited thereto.

When a pressure of 100 MPa to 200 MPa is applied when passing the suspension on a high-pressure homogenizer, the cell walls of bacteria that may be present on the surface of the cellulose fibers are destroyed and sterilized, and the cellulose fibers are decomposed by the pressure to become nanofibers can

At this time, if the pressure of the high-pressure homogenizer is less than 100 MPa, the pulverization degree of the cellulose fibers is weak, so that the cellulose nanofibers and the cellulose fibers may be mixed.

According to one embodiment of the present application, the length of the cellulose nanofibers passing through the high-pressure homogenizer may be 10 nm to 100 μm, but is not limited thereto. Preferably, the length of the cellulose nanofibers may be 10 nm to 100 nm, or 30 μm to 100 μm.

Since the diameter of the cellulose nanofibers is very small, ranging from several to several tens of nanometers, when the length of the cellulose nanofibers is even on the nanometer scale, they may flow into skin pores and cause inflammation. In order to prevent this, while controlling the length of cellulose nanofibers to 10 nm to 100 nm, at the same time adding Mg (OH) ₂ to cosmetics to cope with inflammation, or to reduce the length of cellulose nanofibers to 30 μm to It is necessary to set the cellulose nanofiber to 100 μm to prevent penetration of the cellulose nanofiber into the pores.

In this regard, the length of the cellulose nanofibers passing through the high-pressure homogenizer may be several to several tens of micrometers. However, when the cellulose nanofibers are hydrolyzed in a sulfuric acid environment, the length of the cellulose nanofibers may be shortened.

Specifically, the solution containing the cellulose nanofibers was mixed with a 40 wt% sulfuric acid solution, stirred at 50 ° C. for about 1 hour, and then subjected to a neutralization process and a centrifugation process to obtain cellulose nanofibers of 10 nm to 100 nm can do.

In this regard, the cellulose nanofibers may be related to the duration of the effect of cosmetics to be described later. Specifically, since some of the cellulose nanofibers can connect the porous PLLA nanoparticles to each other, when a cosmetic containing the porous PLLA nanoparticles is applied to the skin, it can remain on the skin surface for a long time without flowing down by the cellulose nanofibers, , which is effective in moisturizing the skin.

Subsequently, the solution and a culture medium containing a stem cell-derived culture medium are mixed to coat the inside and outside of the porous PLLA nanoparticles with the culture material.

In this case, the culture material may be coated on the porous PLLA nanoparticles and/or cellulose nanofibers attached to the surface or inside of the porous PLLA nanoparticles.

According to one embodiment of the present application, the culture material may include exosomes, but is not limited thereto.

According to one embodiment of the present application, the culture medium is selected from the group consisting of vitamin C, tiffenoids, mucin, allatoin, glycolic acid, collagen, elastin, epidermal growth factor (EGF), and combinations thereof It may further include, but is not limited thereto.

The culture medium means that the stem cells are removed from the medium after placing the stem cells in a medium and culturing the stem cells. At this time, the culture medium may include substances such as epidermal cell growth factors as well as exosomes released during the process of culturing the stem cells, and carbon sources, nitrogen sources, inorganic salts, vitamins, etc. contained in the medium can include

According to one embodiment of the present application, the stem cells may include human adipose tissue-derived stem cells, but are not limited thereto. The human adipose tissue-derived stem cells are stem cells derived from adipose tissue collected by liposuction.

The exosome according to the present application is a heterogeneous type of vesicles (extracellular vesicles) produced in cells and released to the outside, and is an endoplasmic reticulum of 30 nm to 150 nm. It is known that the exosome plays a role of transmitting information between cells and can affect energy metabolism of various fat cell tissues.

In this regard, the pH of the culture medium may be 3.5 to 4.5, but is not limited thereto. When the pH of the culture solution is 3.5 to 4.5, it can be confirmed that more exosomes are produced than when cultured in other pH ranges.

According to one embodiment of the present application, the inside and outside of the porous PLLA nanoparticles may be coated with the culture material, but is not limited thereto.

Since pores are formed on the surface of the porous PLLA nanoparticles, the culture material having a smaller size than the pores of the porous PLLA nanoparticles may flow into the porous PLLA nanoparticles and be located inside the porous PLLA nanoparticles. have.

According to one embodiment of the present application, mixing the solution and the culture medium may be performed in an environment having a pH of 7 to 8, but is not limited thereto.

Specifically, the solution is a PBS solution containing porous PLLA nanoparticles, and even when the culture medium is mixed with the PBS solution, the pH can be maintained constant.

The culture solution mixed with the solution containing the porous PLLA nanoparticles may be stem cells removed, but is not limited thereto.

In the step of mixing the solution and the culture medium, when the pH is adjusted to 7 to 8, it can be confirmed that the amount of exosomes remaining without being placed inside and/or outside the porous PLLA nanoparticles after the coating process is finished is small. And, when the solution and the culture solution are mixed after adjusting the pH to less than 7 or more than 8, it can be confirmed that the amount of exosomes coated on the inside and / or outside of the porous PLLA nanoparticles is small or the efficacy is reduced.

According to one embodiment of the present application, the solution and culture medium may be stirred at a speed of 60 rpm to 180 rpm after mixing, but is not limited thereto.

Next, the solution and the hyaluronic acid solution in which TiO ₂ is dissolved are mixed to coat the outside of the porous PLLA nanoparticles with hyaluronic acid.

According to one embodiment of the present application, the hyaluronic acid solution is a group consisting of vitamin C, tiffenoids, mucin, allantoin, glycolic acid, collagen, elastin, epidermal growth factor (EGF) and combinations thereof It may further include those selected from, but is not limited thereto.

Hyaluronic acid according to the present application is a polysaccharide mucous substance, and has excellent moisturizing power, and can maintain skin moisture for a long time. In addition, in the cosmetic according to the present application, hyaluronic acid improves the moisturizing power of the skin to which the cosmetic is applied by coating the porous PLLA nanoparticles coated with the culture material, and the porous PLLA nanoparticles are exposed to the air to decompose or escape from the skin. can refrain from doing so.

According to one embodiment of the present application, the step of mixing the solution and the hyaluronic acid solution may be performed in an environment in which a voltage of 10 kV is applied, but is not limited thereto.

When a solution containing the PLLA nanoparticles coated as the culture material and a solution containing the hyaluronic acid are mixed and a voltage of 10 kV is applied, the hyaluronic acid reacts with the anionic nature of the cellulose nanofibers to form the PLLA The degree of coating the nanoparticles can be improved, and microorganisms added during the mixing process can be removed.

In addition, the hyaluronic acid solution contains TiO ₂ , and the TiO ₂ may be present on or inside a hyaluronic acid layer formed by coating hyaluronic acid on the porous PLLA nanoparticle layer. The TiO ₂ is a material that can block ultraviolet rays, and when the cosmetics are applied to the skin, UV rays can be blocked by TiO ₂ to protect the skin.

According to one embodiment of the present application, the TiO ₂ may be disposed on a layer made of hyaluronic acid formed on the outside of the porous PLLA nanoparticles, but is not limited thereto.

Since the TiO ₂ disposed on the skin is fixed by the cellulose nanofibers and hyaluronic acid of the cosmetic product, TiO ₂ may not flow down even if the skin to which it is applied is sweaty.

At this time, even if the cosmetic is less applied to the skin or time passes after application, the cosmetic can recover even if the skin is damaged by ultraviolet rays because it includes the culture material included inside and outside the porous PLLA nanoparticles. have.

According to one embodiment of the present application, the particle size of the TiO ₂ may be 100 nm to 150 nm, but is not limited thereto. When the TiO ₂ particle size is less than 100 nm, it collides with the exosomes inside the pores of the porous PLLA nanoparticles to release the exosomes from the nanoparticles, or reacts with visible light to generate heat so that the skin coated with cosmetics There may be issues with heating up. In addition, when the TiO ₂ particle size exceeds 150 nm, when the solution and the hyaluronic acid solution are mixed, the porous PLLA nanoparticles and the TiO ₂ particles collide, resulting in a portion of the exosomes of the porous PLLA nanoparticles forming porous PLLA nanoparticles. A problem of separation from the nanoparticles occurred.

According to one embodiment of the present application, after coating the porous PLLA nanoparticles with hyaluronic acid, heat treatment at 50°C and heat treatment at 60°C may be further included, but is not limited thereto.

The heat treatment at 50 ° C is to prevent the hyaluronic acid from being separated from the porous PLLA nanoparticles, and the culture material and the cellulose nanofibers are not supplied with heat by the outermost hyaluronic acid, so that the molecular structure is damaged It may not be.

In addition, the step of heat treatment at 60 ° C is a process of adjusting the TiO ₂ to be disposed on the surface and inside of the hyaluronic acid formed on the porous PLLA nanoparticle layer. Exosomes inside the nanoparticles are destroyed, and when heat treatment is performed at a temperature of less than 60° C., TiO ₂ is separated from the PLLA nanoparticles coated with hyaluronic acid, and the UV blocking effect may be reduced.

In this case, a step of cooling to room temperature may be included between the heat treatment at 50° C. and the heat treatment at 60° C., but is not limited thereto.

In the case of heat treatment at 60 ° C without cooling after the heat treatment at 50 ° C, some of the hyaluronic acid escaped out of the PLLA nanoparticles, whereas when the room temperature cooling step was included, the escape of hyaluronic acid was not confirmed, thereby protecting the skin and tissue of the cosmetic Regeneration may be improved.

According to one embodiment of the present application, the manufacturing method of the cosmetic product may further include mixing the hyaluronic acid-coated porous PLLA nanoparticles with a moisturizer, a solvent, a surfactant, a viscosity modifier, a pH modifier, and a preservative. It may be, but is not limited thereto.

The porous PLLA nanoparticles coated with hyaluronic acid are a type of skin regeneration component included in cosmetics, and by adding the porous PLLA nanoparticles to cosmetics, skin damaged by ultraviolet rays can be regenerated and moisture can be maintained, resulting in skin elasticity. can keep However, since it is difficult to directly apply the porous PLLA nanoparticles to the skin, the porous nanoparticles coated with hyaluronic acid are used as moisturizers, solvents, surfactants, viscosity modifiers, pH modifiers, and various ingredients included in general cosmetics such as preservatives. It is necessary to prepare it in the form of cosmetics in combination with

According to one embodiment of the present application, the cosmetic may include a sunscreen, but is not limited thereto.

Since the sunscreen according to the present application includes not only TiO ₂ but also exosomes, hyaluronic acid, cellulose nanofibers, Mg(OH) ₂ , etc. for sunscreen, it is helpful in improving skin stimulated by ultraviolet rays. It can be, can suppress the occurrence of inflammation in the applied skin, and can maintain moisturizing properties.

According to one embodiment of the present application, the cosmetics may include plant-derived extracts, and the plant-derived extracts include Spanish licorice extract, Lakemosa horseback riding root extract, sewage root extract, black sesame extract, mud mushroom extract, and Angelica gigas. It may include root extract, mulberry bark extract, peony root extract, sorghum root extract, golden extract, black bean extract, oat kernel extract, green tea water, hydrolyzed soybean protein, and wheat protein, but is not limited thereto.

Green tea water according to the present application can help skin care.

In addition, Spanish licorice extract, racemosa rhododendron root extract, rhododendron root extract, black sesame extract, mud mushroom extract, angelica root extract, mulberry bark extract, peony root extract, sorghum root extract, and golden extract help skin care. material that can be given.

According to one embodiment of the present application, the pH adjusting agent may include Mg(OH) ₂ , but is not limited thereto.

The Mg(OH) ₂ can not only adjust the pH of cosmetics, but also suppress inflammation when inflammation occurs in the skin surface epidermal layer and/or inner dermal layer to which the cosmetic is applied. For example, even if the porous PLLA nanoparticles or cellulose nanofibers of the cosmetics penetrate into the skin pores and damage the cell walls of the skin to cause inflammation, the inflammation can be alleviated or suppressed by the Mg(OH) ₂ .

Also, the second aspect of the present application relates to a cosmetic, prepared according to the first aspect.

With respect to the cosmetics according to the second aspect of the present application, detailed descriptions of portions overlapping with those of the first aspect of the present application have been omitted, but even if the description is omitted, the contents described in the first aspect of the present application are the same as those of the second aspect of the present application. can be applied

The above-described problem solving means are merely exemplary and should not be construed as intended to limit the present disclosure. In addition to the exemplary embodiments described above, additional embodiments may exist in the drawings and detailed description of the invention.

The present invention will be described in more detail through the following examples, but the following examples are for illustrative purposes only and are not intended to limit the scope of the present application.

[Example 1]

First, to produce porous PLLA nanoparticles, 200 mg of PLLA was dissolved in 10 ml of acetone, and then the acetone was added dropwise to 150 ml of a PBS solution in which PVA was dissolved at a concentration of 0.4 wt% and the pH was 7 to 8, Porous PLLA nanoparticles were formed by heating at 60° C. while injecting air into the PBS solution at a rate of 30 cc/sec to remove acetone. At this time, the mass of the PLLA, the volume of the acetone and PBS solutions, and the injection amount of air can be adjusted.

In addition, simultaneously with or separately from the process of producing the porous PLLA nanoparticles, a suspension was prepared by mixing 1.5 g of cellulose powder with 100 ml of distilled water, and then the suspension was passed through a high-pressure homogenizer capable of applying a pressure of 150 MPa to obtain a diameter Cellulose nanofibers with a length of 30 µm to 100 nm in nanometers were formed. At this time, when supplying the suspension to the high-pressure homogenizer, air was supplied to the suspension in an amount of 1 cc/sec.

In addition, human adipose tissue-derived stem cells collected through liposuction are placed in a medium with a pH of 4 and cultured for 1 to 2 weeks, and then the adipose-derived stem cells are removed from the medium to obtain a culture medium containing exosomes, a culture material. prepared. At this time, the culture medium may further include an epidermal cell growth factor.

Subsequently, the PBS solution containing the porous PLLA nanoparticles, the cellulose nanofibers, and the culture medium were mixed, the pH was adjusted to 7.5, and then stirred at a speed of 120 rpm to obtain a solution containing the exosome-coated PLLA nanoparticles. prepared.

Subsequently, 1 L of a hyaluronic acid solution containing vitamin C and 100 mg of TiO ₂ having a particle size of 120 nm and a solution containing the exosome-coated PLLA nanoparticles were mixed, and a voltage of 10 kV was applied, A solution containing functional PLLA nanoparticles was formed by first heat treatment at 50°C, cooling to room temperature, and then second heat treatment at 60°C.

Then, on the solution containing the functional PLLA nanoparticles, Mg (OH) ₂ , moisturizers, solvents, surfactants, pH adjusters, viscosity modifiers, substances included in general cosmetics such as preservatives, Spanish licorice extract, racemosa horse Root extract, sagebrush extract, black sesame extract, mud mushroom extract, angelica root extract, mulberry bark extract, peony root extract, sorghum root extract, golden extract, black bean extract, oat kernel extract, green tea water, hydrolyzed soybean A sunscreen was prepared by mixing plant-derived extracts including proteins and wheat proteins. In this case, the functional PLLA nanoparticles may be 2 parts by weight and the plant-derived extract may be 0.1 to 1 part by weight, based on 100 parts by weight of the shampoo.

[Example 2]

Same as Example 1, but after forming the cellulose nanofibers, the solution containing the cellulose nanofibers was mixed with a 40 wt% sulfuric acid solution, stirred at 60 ° C. for about 1 hour, and then neutralized and centrifuged. After passing through, cellulose nanofibers of 10 nm to 100 nm were obtained.

[Comparative Example 1-1]

The same as in Example 1, but a PBS solution in which PVA was not dissolved was used.

[Comparative Example 1-2]

Same as Example 1, but no air was blown over the PBS solution.

[Comparative Example 1-3]

Same as in Example 1, but air was injected into the surface of the PBS solution at a rate of 30 cc/sec.

[Comparative Example 1-4]

Same as in Example 1, but air was injected into the PBS solution at a rate of 5 cc/sec.

[Comparative Example 1-5]

Same as in Example 1, but air was injected into the PBS solution at a rate of 60 cc/sec.

[Comparative Example 1-6]

Same as Example 1, but the heating temperature was set to 80°C.

[Comparative Example 1-6]

Same as Example 1, but the process of removing acetone was omitted.

[Comparative Example 2-1]

Same as Example 1, but the pressure applied in the high-pressure homogenizer was adjusted to 90 MPa.

[Comparative Example 2-2]

Same as Example 1, but the pressure applied in the high-pressure homogenizer was adjusted to 210 MPa.

[Comparative Example 2-3]

Same as Example 1, but the amount of air injected into the suspension supplied to the high-pressure homogenizer was adjusted to 0.9 cc/sec.

[Comparative Example 2-4]

Same as Example 1, but the amount of air injected into the suspension supplied to the high-pressure homogenizer was adjusted to 3.1 cc/sec.

[Comparative Example 3-1]

Same as in Example 1, but pH was adjusted to 1 when culturing adipose-derived stem cells.

[Comparative Example 3-2]

Same as Example 1, but pH was adjusted to 7 when culturing adipose-derived stem cells.

[Comparative Example 3-3]

Same as in Example 1, but pH was adjusted to 10 when adipose-derived stem cells were cultured.

[Comparative Example 4-1]

Same as Example 1, but the step of applying a voltage of 10 kV was omitted when forming the functional PLLA nanoparticles.

[Comparative Example 4-2]

Same as Example 1, but the process of heat treatment at 50° C. was omitted when forming the functional PLLA nanoparticles.

[Comparative Example 4-3]

Exosome-coated PLLA nanoparticles were heat-treated in the same manner as in Example 1, but without hyaluronic acid.

[Comparative Example 4-4]

Same as Example 1, but the process of forming cellulose nanofibers was omitted, and only the PBS solution and the culture medium were mixed.

[Comparative Example 5-1]

Same as in Example 1, but the pH was adjusted to 6 when mixing the solution containing the porous PLLA nanoparticles and the culture medium.

[Comparative Example 5-2]

Same as in Example 1, but the pH was adjusted to 9 when mixing the solution containing the porous PLLA nanoparticles and the culture medium.

[Comparative Example 6-1]

Same as Example 1, but without adding TiO ₂ .

[Comparative Example 6-2]

Same as Example 1, but the particle size of TiO ₂ was adjusted to 160 nm.

[Comparative Example 6-3]

Same as Example 1, but the particle size of TiO ₂ was adjusted to 80 nm.

[Comparative Example 6-4]

Same as Example 1, but the secondary heat treatment temperature was adjusted to 80°C.

[Comparative Example 6-5]

Same as Example 1, but the secondary heat treatment temperature was adjusted to 50°C.

[Example 6-6]

Same as Example 1, but the first heat treatment and the second heat treatment were performed consecutively.

[Experimental Example 1]

Porous PLLA nanoparticles were compared by comparing Example 1 and Comparative Examples 1-1 to 1-5.

[Table 1]

In Table 1, the average particle size and average pore size were calculated through dynamic light scattering and SEM imaging, and the yield was the ratio between the mass obtained by centrifuging porous PLLA nanoparticles from a PBS solution and 200 mg of PLLA. means

In Table 1, when air was not injected or air was injected only on the surface of the PBS solution as in Comparative Examples 1-2 and 1-3, porous PLLA nanoparticles as well as poreless PLLA nanoparticles were formed, among which pores Since the number of PLLA nanoparticles without these was found to be higher, we did not measure the average pore size.

Referring to Table 1, in the case of Comparative Example 1-1 without using PVA, the size of the porous PLLA nanoparticles increased due to aggregation, and as a result, it was confirmed that the rate of absorption into the skin as a cosmetic decreased.

In Comparative Examples 1-4, the average particle size and average pore size were measured larger than those of Example 1 due to a small amount of air injection, so the skin absorption rate may be lower than that of the porous PLLA nanoparticles of Example 1. In addition, after mixing the porous PLLA nanoparticles and the culture solution according to Example 1 and Comparative Examples 1-4, when the exosome-coated PLLA nanoparticles were extracted by centrifugation, the amount of exosomes in the remaining solution was the same as in Comparative Example 1. There were more solutions of -4. This is because the larger the pores, the more exosomes are injected into the porous PLLA nanoparticles, so the amount of exosomes released to the outside increases. It was confirmed that the efficiency was reduced because it could be produced.

On the other hand, in the case of Comparative Examples 1-5, the average particle size and average pore size were measured smaller than those of Example 1, but the amount of air supplied was large, so the porous PLLA nanoparticles were decomposed, and the PLLA was dissolved in the PBS solution.

On the other hand, in the case of Comparative Examples 1-6, the average pore size was smaller than that of Example 1, which was confirmed as a phenomenon caused by a sharp decrease in the solubility of air in the solution. However, it was confirmed that the porous PLLA nanoparticles of Comparative Examples 1-6 were less effective than those of Example 1 because culture materials such as exosomes could not enter therein.

On the other hand, in the case of Comparative Example 1-7, the process of removing acetone was omitted, and considering that the boiling point of acetone is 56 ° C., it was not removed even in the heat treatment process described later. The problem of skin damage was confirmed on skin with cosmetics applied.

[Experimental Example 2]

The sizes of cellulose nanofibers prepared by the processes according to Example 1 and Comparative Examples 2-1 to 2-4 were compared.

The cellulose nanofibers of Example 1 had an average diameter of 30 nm and an average length of 35 μm, but the nanofibers of Comparative Example 2-1 had an average diameter of 1 μm and an average length of 120 μm. Insufficient, the nanofibers of Comparative Example 2-2 had an average diameter of 29 nm and an average length of 34 μm, and the average diameter and length were measured within the error range while consuming more energy than in Example 1. In addition, in the case of Comparative Example 2-3, the average diameter was measured as several micrometers and the average length was 137 μm, as in the case of not injecting air. In addition, in the case of Comparative Examples 2-4, nanofibers having an average diameter and average length similar to those of Example 1 were prepared, but the suspension containing the nanofibers escaped from the flow path in the process of passing through the high-pressure homogenizer, or when discharged The problem of partial breakage of the device due to the splashing of the suspension due to sudden depressurization was continuously confirmed.

[Experimental Example 3]

The exosome contents of Example 1 and Comparative Examples 3-1 to 3-3 were analyzed.

When the exosome content of Example 1 was measured as 1, the content of Comparative Example 3-1 was 0.7, the content of Comparative Example 3-2 was 0.6, and the content of Comparative Example 3-3 was about 0.6, Comparative Example 3-1 Since the content of exosomes in the cultures of 3-3 to 3-3 was lower than the content of exosomes in the cultures of Example 1, the efficacy of cosmetics, such as skin regeneration, was low.

[Experimental Example 4]

Example 1 and Comparative Examples 4-1 to 4-3 were compared.

In the case of Comparative Example 4-1, the process of forming functional PLLA nanoparticles by omitting the voltage application process took twice as much time as in Example 1, and during centrifugation, coating on the exosome-coated PLLA nanoparticles Undetected cellulose nanofibers were detected.

In addition, unlike the functional PLLA nanoparticles of Example 1, the functional PLLA nanoparticles of Comparative Example 4-2 simply placed hyaluronic acid on the porous PLLA nanoparticles coated with exosomes and epidermal growth factors, It can be seen that hyaluronic acid and functional PLLA nanoparticles are separated while flowing, and the efficacy is reduced.

In addition, in the case of Comparative Example 4-3, unlike Example 1, there is no hyaluronic acid, so heat directly acts on exosomes, cellulose nanofibers, and epidermal cell growth factors during heat treatment, so that skin moisturizing, etc. performance was low.

In addition, in the case of Comparative Examples 4-4, unlike Example 1, there is no cellulose nanofiber, so compared to the cosmetic of Example 1, performance such as skin moisturizing is low, viscosity is low, and the duration of the effect is short. I could see that it was short.

[Experimental Example 5]

In the experiments of Example 1, Comparative Example 5-1 and Comparative Example 5-2, the PBS solution containing the porous PLLA nanoparticles and the culture medium were mixed, and the porous PLLA nanoparticles were separated from the solution through centrifugation, It was observed whether exosomes were included in the remaining solution.

As a result of observation, exosomes were detected at a concentration of 10 μg/ml in the remaining solution of Example 1, whereas 157 μg/ml and 163 μg/ml were detected in the remaining solutions of Comparative Example 5-1 and Comparative Example 5-2, respectively. was detected, and considering that 451 μg/ml of exosomes was detected in the culture medium before mixing with the PBS solution, as in Comparative Example 5-1 and Comparative Example 5-2, a solution containing porous PLLA nanoparticles and exosomes When mixing the culture medium, the closer the pH is to neutral, the more the exosomes can be attached to the inside and outside of the porous PLLA nanoparticles.

[Experimental Example 6]

The sunscreens of Example 1 and Comparative Examples 6-1 to 6-6 were compared.

As a result of comparison, unlike Example 1, Comparative Example 6-1 did not have UV blocking performance, and in Comparative Example 6-2, as the nanoparticles and TiO ₂ collided, the part made of hyaluronic acid was damaged and exosomes leaked out. The elasticity of the skin to which sunscreen is applied may decrease.

In addition, in the case of Comparative Example 6-3, since the size of the TiO ₂ particles is smaller than that of Example 1, it can be confirmed that the surface plasmon resonance phenomenon occurs due to light and the temperature of the applied skin increases.

In addition, in the case of Comparative Example 6-4 and Comparative Example 6-5, the exosomes inside the porous PLLA nanoparticles are decomposed by heat by heat treatment at high temperature, or the PLLA nanoparticles coated with TiO-- ₂ hyaluronic acid by heat treatment at low temperature There was a problem that it was not fixed on the particle and came off.

In addition, in the case of Comparative Examples 6-6, since the room temperature cooling process was not performed between the first heat treatment process and the second heat treatment process, after the hyaluronic acid heated by the first heat treatment process was coated on the porous PLLA nanoparticles, Since the secondary heat treatment was performed in an unfixed state, the heat applied during the secondary heat treatment process heated the inside of the porous PLLA nanoparticles to decompose the exosomes, thereby degrading the skin regeneration performance.

The above description of the present application is for illustrative purposes, and those skilled in the art will understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present application. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present application is indicated by the following claims rather than the detailed description above, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts thereof should be construed as being included in the scope of the present application.

Claims (8)

As for the manufacturing method of cosmetics,
Preparing a solution containing porous PLLA nanoparticles;
Forming cellulose nanofibers;
coating the inside and outside of the porous PLLA nanoparticles with the culture medium by mixing the solution and a culture medium containing a stem cell-derived culture material; and
coating the outside of the porous PLLA nanoparticles with hyaluronic acid by mixing the solution and the hyaluronic acid solution in which TiO ₂ is dissolved;
including,
The TiO ₂ is disposed on a layer made of hyaluronic acid formed on the outside of the porous PLLA nanoparticles,
In the manufacturing method of cosmetics,
The step of preparing a solution containing the porous PLLA nanoparticles includes dissolving PLLA in acetone, dropping the acetone in which PLLA is dissolved into a PBS solution in which PVA is dissolved and having a pH of 7 to 8, and the PBS. Injecting air into the solution at 30 cc/sec and heating at 60 ° C,
The forming of the cellulose nanofibers includes mixing cellulose powder and distilled water to prepare a suspension, injecting air into the suspension at 1 cc/sec and passing it through a high-pressure homogenizer at a pressure of 150 MPa, ,
The culture medium is formed by placing human adipose tissue-derived stem cells in a medium having a pH of 4, culturing for 1 to 2 weeks, and then removing the human adipose tissue-derived stem cells from the medium. include,
In the step of coating the inside and outside of the porous PLLA nanoparticles with the culture material, mixing the PBS solution containing the porous PLLA nanoparticles, the cellulose nanofibers, and the culture medium, the pH of the mixed material is 7.5 Adjusting to , and stirring the mixed material at a speed of 120 rpm,
The step of coating the outside of the porous PLLA nanoparticles with hyaluronic acid is mixing the hyaluronic acid solution containing TiO ₂ and the solution containing the PLLA nanoparticles coated as the culture material, and applying a voltage of 10 kV to the solution. Applying a, and heat-treating the solution at 50 ° C.,
Manufacturing method of cosmetics.
According to claim 1,
The porous PLLA nanoparticles have a diameter of 50 nm to 200 nm, and the porous PLLA nanoparticles have a pore diameter of 10 nm to 60 nm.
delete delete delete According to claim 1,
After the porous PLLA nanoparticles are coated with hyaluronic acid, further comprising the step of heat treatment at 60 ° C., a method for producing cosmetics.
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