KR102468770B1 - Soil management sheet comprising lactobachillus complex and plant byproducts and biological soil management method of soil pollution - Google Patents

Abstract

The land improvement sheet containing complex lactic acid bacteria and plant by-products of the present invention includes a matrix layer containing natural powder and complex lactic acid bacteria and a mineral layer formed under the matrix layer.

Description

Land improvement sheet containing complex lactic acid bacteria and plant by-products and biological land improvement method for soil contamination using the same

The present invention relates to a land improvement sheet containing complex lactic acid bacteria and plant by-products and a biological land improvement method for soil contamination using the same.

In general, more than 50% of soil in Korea is composed of granite and granite, and the soil is gradually becoming acidic due to soil loss due to heavy rains in summer, subsequent leaching of calcium, and excessive use of chemical fertilizers and pesticides. .

In addition, in various industrial complexes, gas stations, military bases, etc. where a large amount of oil is used, a significant amount of oil leaks out due to negligence and inevitable reasons during transportation, sale, storage, and use of oil, contaminating the soil.

The use of chemical fertilizers and pesticides not only acidifies the soil and reduces the natural soil purification ability, but also damages crops by breaking the physical and chemical balance of the soil and lowering the soil's regeneration ability. In addition, oil is discarded without being safely treated or permeated into the soil, causing soil contamination, which has a great adverse effect on the human body and the environment.

Accordingly, there is a need for a land improvement sheet capable of preventing soil acidification and oil contamination.

An object of the present invention is to provide a land improvement sheet containing complex lactic acid bacteria and plant by-products capable of preventing soil acidification and a biological land improvement method for soil contamination using the same.

Another object of the present invention is to provide a land improvement sheet containing complex lactic acid bacteria and plant by-products capable of biologically preventing oil-contaminated soil, and a biological land improvement method for soil contamination using the same.

The above and other objects of the present invention can all be achieved by the present invention described below.

One aspect of the present invention relates to a land improvement sheet containing complex lactic acid bacteria and plant by-products.

According to one embodiment, the land improvement sheet includes a matrix layer containing natural powder and complex lactic acid bacteria; and a mineral layer formed under the matrix layer.

According to another embodiment, the land improvement sheet includes a mineral layer; a second matrix layer; and a first matrix layer may be sequentially stacked, the first matrix layer may contain natural powder and complex lactic acid bacteria, and the second matrix layer may contain natural powder.

According to another embodiment, the land improvement sheet includes a mineral layer; a second matrix layer; And a first matrix layer is sequentially stacked, and the first matrix layer and the second matrix layer may include natural powder and complex lactic acid bacteria.

In addition, the natural powder may include one or more plant by-products of rice bran powder and coconut shell powder.

In addition, the complex lactic acid bacteria may include at least one of a lactic acid bacteria body and a yeast cell body.

In addition, the lactic acid bacteria body, Lactobacillus parafarraginis, Lactobacillus paracasei subsp. Tolerans, Lactobacillus buchneri, Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus, Lactobacillus vaccinostercus, Acetobacter lovaniensis and Acetobacter peroxydans.

In addition, the yeast cell body may include one or more of Pichia fermentans, Candida ethanolica, Lactobacillus buchneri, and Saccharomycopsis schoenii.

In addition, the matrix layer containing the natural powder and complex lactic acid bacteria may be prepared by mixing 100 parts by weight of natural powder, 5 to 20 parts by weight of the complex lactic acid bacteria composition, and 10 to 30 parts by weight of a binder.

In addition, the composite lactic acid bacteria composition may include 0.1 to 5 parts by weight of complex lactic acid bacteria powder and 1 to 30 parts by weight of molasses based on 100 parts by weight of water.

In addition, the mineral layer may include 25 to 40 parts by weight of shell powder, 5 to 15 parts by weight of a binder, and 10 to 30 parts by weight of water.

In addition, the binder may include at least one of starch and flour.

Also, the land improvement sheet may be a roll-type land improvement sheet or a plate-type land improvement sheet.

Another aspect of the present invention relates to a biological land improvement method for soil contamination using a land improvement sheet containing complex lactic acid bacteria and plant by-products.

The present invention has the effect of providing a land improvement sheet containing complex lactic acid bacteria and plant by-products capable of biologically preventing soil acidification and oil-contaminated soil, and a biological land improvement method for soil contamination using the same.

1 is a cross-sectional view of a land improvement sheet according to one embodiment of the present invention.
2 to 4 are cross-sectional views of a land improvement sheet according to another embodiment of the present invention.
5 schematically shows a roll-type land improvement sheet according to another embodiment of the present invention.
6 schematically shows a plate-shaped land improvement sheet according to another embodiment of the present invention.

Hereinafter, the present invention will be described in more detail.

In describing the present invention, if it is determined that a detailed description of related known technologies may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted.

When 'includes', 'has', 'consists of', etc. mentioned in this specification is used, other parts may be added unless 'only' is used. In the case where a component is expressed in the singular, the case including the plural is included unless otherwise explicitly stated.

In addition, in interpreting the components, even if there is no separate explicit description, it is interpreted as including the error range.

In addition, in the present specification, 'X to Y' representing a range means 'X or more and Y or less'.

Land improvement sheet containing complex lactic acid bacteria and plant by-products

1 to 3 are cross-sectional views of a land improvement sheet 1000 including complex lactic acid bacteria and plant by-products according to an embodiment of the present invention. Referring to FIG. 1, the land improvement sheet 1000 according to one embodiment of the present invention may include a matrix layer 100 containing natural powder and complex lactic acid bacteria and a mineral layer 200 formed under the matrix layer. have.

Referring to FIG. 2, in the land improvement sheet 1000 according to another embodiment of the present invention, a mineral layer 200, a second matrix layer 130, and a first matrix layer 110 are sequentially stacked, and the first matrix layer 110 is sequentially stacked. The first matrix layer 110 may include natural powder and complex lactic acid bacteria, and the second matrix layer 130 may include natural powder.

Referring to FIG. 3, in the land improvement sheet 1000 according to another embodiment of the present invention, a mineral layer 200, a second matrix layer 170, and a first matrix layer 150 are sequentially stacked, and the first matrix layer 150 is sequentially stacked. The first matrix layer 150 and the second matrix layer 170 may contain natural powder and complex lactic acid bacteria.

Up until now, land improvers in the form of powder have been mainly used to improve soil. In the case of using a land improvement agent in powder form, there is a limit to uniformly applying the land improvement agent on the soil.

The land improvement sheet 1000 of the present invention includes the matrix layers 100, 110, 130, 150, and 170 formed in the form of a sheet, so that the land improvement sheet 1000 can be easily and uniformly installed on the soil. Soil can be improved.

The natural powder may include at least one plant by-product of rice bran powder and coconut shell powder.

The rice hull is a waste generated in rural areas, and can oxidize and decompose various organic substances contained in the soil to maintain the content of corrosive substances in the soil at an appropriate level, improve the soil to be suitable for living organisms, and various remaining in the soil. It has the advantage of improving the aeration and water retention of soil by activating beneficial microorganisms and adsorbing and removing harmful microorganisms. The chaff may be chaff charcoal, but is not limited thereto.

The coconut shell is a pure natural organic material, environmentally friendly, and has excellent drainage and breathability. In addition, it is stable under oxidizing conditions and contains a significant amount of organic nutrients and trace elements, so nutrients, moisture and oxygen can be smoothly supplied to the roots of plants to be raised.

The matrix layer may include the rice hull powder and the coconut shell powder having different average particle sizes. In this case, the porosity of the matrix layer is improved to secure enough space for the multi-lactic acid bacteria to inhabit, thereby improving the activity of the multi-lactic acid bacteria and improving the soil improvement efficiency of the multi-lactic acid bacteria.

For example, the average particle size of the rice hull powder may be 0.5 mm to 2 mm, specifically 1 mm to 2 mm, and more specifically 1.2 mm to 2 mm, and the average particle size of the coconut powder is 0.01 mm. mm or more and less than 0.5 mm, specifically, may be 0.01 mm or more and less than 0.3 mm.

In addition, the weight ratio of the rice hull powder and the coconut shell powder may be 1: 1 to 3: 1, specifically 1.5: 1 to 2.5: 1, and it is easy to form a matrix layer in the form of a sheet in the weight ratio range, It is effective in neutralizing acidified soil and purifying oil-contaminated soil, and by supplying sufficient nutrients, it can activate the growth of plants such as seedlings and prevent various diseases and pests. In addition, it is possible to secure a space in which the multi-lactic acid bacteria can inhabit, thereby increasing the soil reforming effect of the multi-lactic acid bacteria.

The complex lactic acid bacteria may include at least one of lactic acid bacteria and yeast, and specifically, the lactic acid bacteria are Lactobacillus parafarraginis, Lactobacillus paracasei subsp. Tolerans, Lactobacillus buchneri, Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus, Lactobacillus vaccinostercus, Acetobacter lovaniensis, and Acetobacter peroxydans , and at least one of Pichia fermentans, Candida ethanolica, and Saccharomycopsis schoenii . have.

The Lactobacillus parafarraginis can defend against infection from other harmful bacteria and help crops absorb nutrients to prevent plague, and the Lactobacillus paracasei subsp. Tolerans can inhibit the activity of Escherichia coli and play an important role in crop growth by producing phosphatase. The Lactobacillus harbinensis has a wide growth temperature range and can stably maintain antibacterial action, and the Lactobacillus vaccinostercus has an excellent effect of preventing infectious viruses and pathogenic microorganisms and reducing odors such as ammonia and hydrogen sulfide.

The Lactobacillus parafarraginis, Lactobacillus paracasei subsp. Tolerans, Lactobacillus harbinensi, Lactobacillus vaccinostercus, and complex lactic acid bacteria including Lactobacillus buchneri , Lactobacillus perolens , Lactobacillus rhamnosus, Acetobacter lovaniensis, Acetobacter peroxydans, Pichia fermentans, Candida ethanolica and Saccharomycopsis schoenii can supply sufficient nutrients to crops and In addition, it is possible to obtain the effect of improving the fertility of the land, and in particular, the effect of protecting crops from diseases or germs by antagonistic microorganisms can be obtained. In particular, it is possible to prevent environmental pollution and the destruction of the ecosystem as it is possible to protect crops from disease or pests without using chemically manufactured pesticides.

에서 72시간 내지 120시간 동안 배양하여 생성된 복합유산균용액을 원심분리하여 상등액이 분리된 유산균균체 및 효모균균체를 얻어 동결 또는 동결건조함으로써 분말상(powder)으로 수득할 수 있다.The complex lactic acid bacteria are Lactobacillus parafarraginis, Lactobacillus paracasei subsp. Tolerans, Lactobacillus buchneri, Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus, Lactobacillus vaccinostercus, Acetobacter lovaniensis and Acetobacter peroxydans were 2.2 Х 10 ⁸ cfu/ml, and yeasts Pichia fermentans, Candida ethanolica and Saccharomycopsis schoenii were ^2.4 Х cfu/ml. 25 by inoculation

The multi-lactic acid bacteria solution produced by culturing for 72 to 120 hours can be centrifuged to obtain lactic acid bacteria and yeast cells from which the supernatant is separated, and can be obtained in powder form by freezing or freeze-drying.

The matrix layer containing the natural powder and the mixed lactic acid bacteria may be prepared by mixing the natural powder, the composite lactic acid bacteria composition, and the binder, and then going through molding and drying steps.

The matrix layer containing the natural powder and the multi-lactic acid bacteria may be prepared by mixing 100 parts by weight of the natural powder, 5 to 20 parts by weight of the multi-lactic acid bacteria composition, and 10 to 30 parts by weight of a binder.

The complex lactic acid bacteria composition may include water, complex lactic acid bacteria powder and molasses.

The complex lactic acid bacteria composition may include 0.1 to 5 parts by weight of complex lactic acid bacteria powder, specifically 0.5 to 3 parts by weight, based on 100 parts by weight of water. In the above content range, the activity of the multi-lactic acid bacteria can be increased and the death of the multi-lactic acid bacteria can be prevented, so that the effect of improving the land due to the multi-lactic acid bacteria can be sufficiently achieved, as well as the cost required can be reduced.

The composite lactic acid bacteria composition may include 1 to 30 parts by weight of molasses, specifically 5 to 25 parts by weight, based on 100 parts by weight of water, and is suitable for the activation of the composite lactic acid bacteria in the above range to show a soil reforming effect.

The complex lactic acid bacteria composition may be 5 to 20 parts by weight, specifically 7 to 12 parts by weight, based on 100 parts by weight of the natural powder, and a sheet-shaped matrix layer may be formed with the natural powder in the above range, and the complex lactic acid bacteria composition of soil reforming effect.

The binder may be starch or flour, and the flour may be in the form of at least one powder selected from the group consisting of soybean meal, corn gluten, jade germ meal, ginseng meal, zucchini meal, and rapeseed meal. For example, the binder may be soybean meal.

The binder may be 10 to 30 parts by weight, specifically 15 to 25 parts by weight, and within the above range, durability and moldability of the matrix layer may be improved.

The mineral layer 200 may be formed below the matrix layer, and may include 25 to 40 parts by weight of shell powder, 5 to 15 parts by weight of a binder, and 10 to 30 parts by weight of water. In the present invention, the parts by weight of components included in the mineral layer 200, for example, shell powder, binder, etc., refer to their relative contents based on 100 parts by weight of natural powder.

The shell powder is pulverized shells of aquatic products such as oysters, clams and scallops, and can be used as a fertilizer because it alkalizes the soil and promotes the growth of useful microorganisms in the soil by mixing starch and starch. In addition, shell powder contains a large amount of lime components, and these lime components are valuable enough to be classified as four elements of fertilizer along with nitrogen (N), phosphoric acid (P), and potassium (K). In particular, as the acidification of soil intensifies, it is suitable for use as a soil conditioner, and has the advantage of having no side effects and environmental pollution compared to chemically synthesized fertilizers. The shell powder may be oyster shell, but is not limited thereto.

The shell powder may be 25 to 40 parts by weight, specifically 30 to 35 parts by weight, and acidic soil can be neutralized in the above range.

The shell powder may have an average particle size of 0.1 μm to 5 μm. It can be mixed uniformly within the above range.

As another embodiment of the present invention, the mineral layer 200 may further include shungite. The shunggit is a natural mineral with a carbon content of 30% to 40% and a silicate content of 60% to 70%. It contains more than 20 useful trace elements useful for humans and living things, such as sodium, magnesium, manganese, iron, copper and zinc. Carbon in shunggit has a fullerene and higher order fullerene structure with small grains of 2 nm to 30 nm, so it has adsorption power to purify water and air from organic and inorganic compounds, and also has high sterilization power and the ability to remove contamination from water or oil. and silicates can neutralize acidified soils.

Therefore, shungit has the advantage of being applicable to various industrial fields as it functions as a purification action of contaminated water, radiation neutralization action, air purification action, fertilizer effect in agriculture, and preservation and disinfectant in plants and vegetables.

The shunggit may be 0.1 to 5 parts by weight, specifically 0.5 to 3 parts by weight, and within this range, harmful substances contained in the soil can be effectively adsorbed and removed, the soil can be neutralized, and oil-contaminated soil can be purified. . Also, it is effective in removing odors from the soil.

The shunggit may have an average particle size of 0.5 μm to 50 μm. Within the above range, the handling property is good, and the mixing property with the binder, the adhesion property, and the coating property are excellent.

The mineral layer 200 may be formed by mixing the shell powder and shunggit with water, applying the binder to the lower part of the matrix layer 100, and then drying at room temperature.

The binder may be included in 5 to 15 parts by weight, and the water may be included in 7 to 15 parts by weight, and minerals containing shell powder and shungite capable of alkalizing the soil and adsorbing harmful substances to purify oil-contaminated soil in the above range The layer 200 may be stably formed. In addition, durability of the mineral layer may be improved.

The binder may include at least one of starch or flour.

The flour may be in the form of at least one powder selected from the group consisting of soybean meal, corn gluten, jade germ meal, ginseng meal, zucchini meal, and rapeseed meal. For example, the binder may be soybean meal.

4 is a cross-sectional view of a land improvement sheet 1100 including complex lactic acid bacteria and plant by-products according to another embodiment of the present invention.

When the land improvement sheet 1100 is wound in a roll type, the upper surface of the mattress layer 100 and the lower surface of the mineral layer 200 are formed to prevent mutual adhesion between the mattress layer 100 and the mineral layer 200. The release layer 300 may be formed by attaching a release film or release paper to one or more surfaces.

The release film or release paper may be a material that is easily separated from the matrix layer and the mineral layer or a material including a coating, but is not limited thereto.

Since the rest of the components except for the release layer 300 are the same as those described in FIGS. 1 to 3, detailed descriptions thereof will be omitted.

5 schematically shows a roll-type land improvement sheet 1100 according to another embodiment of the present invention. The roll-type land improvement sheet 1100 is obtained by winding a land improvement sheet and rolling it into a roll.

The matrix layer 100 of the roll-type land improvement sheet 1100 may have a thickness of 7 mm to 12 mm, and within the thickness range, the matrix layer 100 may be stored in a roll form without damage.

Since the remaining components of the roll-type land improvement sheet 1100 are the same as those described in FIGS. 1 to 4, detailed descriptions thereof are omitted.

6 schematically shows a plate-shaped land improvement sheet 1200 according to another embodiment of the present invention. The plate-shaped land improvement sheet 1200 may have a width of 0.5 m to 2 m, specifically 0.7 m to 1.8 m, and a length of 0.5 m to 2 m, specifically 0.7 m to 1.8 m.

In the plate-shaped land improvement sheet 1200, the matrix layer 100 may have a thickness of 12 mm to 20 mm, and in the above thickness range, the effect of the natural powder contained in the matrix layer 100 may be exhibited, and the complex lactic acid bacteria may be present. It can be suitable as an environment in which activity can be displayed.

Since the remaining components of the plate-shaped land improvement sheet 1200 are the same as those described in FIGS. 1 to 4, detailed descriptions thereof are omitted.

Hereinafter, the configuration and operation of the present invention will be described in more detail through preferred embodiments of the present invention. However, this is presented as a preferred example of the present invention and cannot be construed as limiting the present invention by this in any sense.

Contents not described herein can be technically inferred by those skilled in the art, so descriptions thereof will be omitted.

Example

Example 1

Natural powder containing 2 kg of rice hull powder with an average particle size of 1.5 mm and 1 kg of coconut shell powder with an average particle size of 0.1 mm, 300 g of the multi-lactic acid bacteria composition, and 600 g of soybean meal were mixed and then molded to form the natural powder and multi-lactic acid bacteria with a thickness of 7 mm. A matrix layer containing was created.

After mixing 300 g of soybean meal and 400 g of water, 1 kg of shell powder having an average particle size of 5 ㎛ was added and stirred again, then applied to the lower surface of the matrix and dried at room temperature for 24 to 72 hours to obtain a mineral with a thickness of 2 mm. Layers were created to produce roll-type land improvement sheets.

The composite lactic acid bacteria composition was prepared by mixing 38 g of molasses with 256 g of water, and then mixing 6 g of composite lactic acid bacteria powder.

As a result of isolation and identification of complex lactic acid bacteria by molecular biological techniques, Lactobacillus parafarraginis and Lactobacillus paracasei subsp. Tolerans, Lactobacillus buchneri, Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus, Lactobacillus vaccinostercus, Acetobacter lovaniensis, Acetobacter peroxydans, Pichia fermentans, Candida ethanolica, Lactobacillus buchneri and Saccharomycopsis schoenii .

Comparative Example 1

Natural powder containing 2 kg of rice hull powder with an average particle size of 1.5 mm and 1 kg of coconut shell powder with an average particle size of 0.1 mm was molded to create a matrix layer containing natural powder with a thickness of 7 mm.

After mixing 300 g of soybean meal and 400 g of water, 1 kg of shell powder having an average particle size of 5 ㎛ was added and stirred again, then applied to the lower surface of the matrix and dried at room temperature for 24 to 72 hours to obtain a mineral with a thickness of 2 mm. Layers were created to produce roll-type land improvement sheets.

Comparative Example 2

Natural powder containing 2 kg of rice hull powder with an average particle size of 1.5 mm and 1 kg of coconut shell powder with an average particle size of 0.1 mm, 300 g of the multi-lactic acid bacteria composition, and 600 g of soybean meal were mixed and then molded to form the natural powder and multi-lactic acid bacteria with a thickness of 7 mm. A roll-type land improvement sheet was prepared by creating a matrix layer containing

The composite lactic acid bacteria composition was prepared by mixing 38 g of molasses with 256 g of water, and then mixing 6 g of composite lactic acid bacteria powder.

As a result of isolation and identification of complex lactic acid bacteria by molecular biological techniques, Lactobacillus parafarraginis and Lactobacillus paracasei subsp. Tolerans, Lactobacillus buchneri, Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus, Lactobacillus vaccinostercus, Acetobacter lovaniensis, Acetobacter peroxydans, Pichia fermentans, Candida ethanolica, Lactobacillus buchneri and Saccharomycopsis schoenii .

Determination of chemical properties of soil

After placing the soil improvement sheet prepared according to Examples and Comparative Examples on the soil for 30 days, the soil within 10 cm was prepared as a sample, and the pH was measured at a sample: distilled water ratio of 1: 5, and the organic matter content was Tyurin law, available phosphoric acid in soil is Bray No. After extraction by method 1, it was measured by a colorimetric method using vanadomomolybdic acid, and is shown in Table 1 below.

soil fertility pH P ₂ O ₅ (mg/kg ^-1 ) SiO ₂ (mg/kg ^-1 ) Example 1 32 165 6.4 Comparative Example 1 20 141 6.4 Comparative Example 2 22 144 5.7

As shown in Table 1, the land improvement sheet of Example 1 including a mineral layer and a matrix layer containing natural powder and complex lactic acid bacteria in Example 1 and Comparative Example 1 including a mineral layer, the acidity of the soil It can be seen that the pH value decreases, that is, the pH value increases. In addition, it can be seen that effective phosphoric acid and SiO ₂ representing soil fertility are increased compared to the land improvement sheets of Comparative Example 1 lacking complex lactic acid bacteria and Comparative Example 2 lacking mineral layer.

Soil Cleanliness Measurement

The soil improvement sheet prepared in Example 1 was placed on oil-contaminated soil for 14 days. After collecting the oil-contaminated soil, the total petroleum hydrocarbons (TPH) concentration is shown in Table 4 below by requesting an official test period. The TPH concentration means the remaining TPH concentration in the soil.

TPH concentration (mg/kg soil) purification efficiency before experiment after experiment Example 1 23,480 11,975 49%

As shown in Table 2, it can be seen that the total petroleum hydrocarbons (TPH) concentration of the soil decreases when the land improvement sheet including the matrix layer and the mineral layer containing natural powder and complex lactic acid bacteria is applied.

Although the embodiments of the present invention have been described above, the present invention is not limited to the above embodiments, but may be manufactured in various different forms, and those skilled in the art to which the present invention belongs can understand the technical spirit of the present invention. However, it will be understood that it may be embodied in other specific forms without changing its essential features. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

100: matrix layer 110, 150: first matrix layer
130, 170: second matrix layer 200: mineral layer
300: release layer
1000. 1100: land improvement sheet

Claims (8)

delete mineral layer; a second matrix layer; and a first matrix layer is sequentially stacked;
The first matrix layer includes natural powder and complex lactic acid bacteria,
The second matrix layer contains natural powder,
The natural powder includes rice bran powder and coconut shell powder in a weight ratio of 1: 1 to 3: 1,
The complex lactic acid bacteria include at least one of lactic acid bacteria and yeast cells,
The lactic acid bacteria body, Lactobacillus parafarraginis, Lactobacillus paracasei subsp. Tolerans, Lactobacillus buchneri, Lactobacillus harbinensis, Lactobacillus perolens, Lactobacillus rhamnosus, Lactobacillus vaccinostercus, Acetobacter lovaniensis, and Acetobacter peroxydans;
The yeast strains include Pichia fermentans, Candida ethanolica, Lactobacillus buchneri and Saccharomycopsis schoenii,
The first matrix layer is prepared by mixing 5 to 20 parts by weight of the multi-lactic acid bacteria composition and 10 to 30 parts by weight of the peel powder with respect to 100 parts by weight of the natural powder,
The complex lactic acid bacteria composition, 100 parts by weight of water; with respect to, 0.1 to 5 parts by weight of complex lactic acid bacteria powder; and 1 to 30 parts by weight of molasses;
The mineral layer, 25 to 40 parts by weight of shell powder; 5 to 15 parts by weight of a binder; and 10 to 30 parts by weight of water;
A land improvement sheet containing a.
delete delete delete delete delete A biological land improvement method for soil contamination using the land improvement sheet of claim 2.

Images