KR102461765B1 - SNP marker set for identifying strawberry cultivars and method for identifying strawberry cultivars using the same - Google Patents
SNP marker set for identifying strawberry cultivars and method for identifying strawberry cultivars using the same Download PDFInfo
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Abstract
본 발명은 딸기 순도검정 및 품종 판별용 마커 세트 및 이의 용도에 관한 것으로, 딸기의 순도검정 및 품종 판별을 신속하고 정확하게 판단할 수 있다.The present invention relates to a marker set for strawberry purity test and variety discrimination and uses thereof, and it is possible to quickly and accurately determine strawberry purity test and variety discrimination.
Description
본 발명은 딸기 순도검정 및 품종 판별용 마커 세트 및 이의 용도에 관한 것이다.The present invention relates to a marker set for strawberry purity test and variety discrimination, and uses thereof.
딸기(Fragaria × ananassa Duch.)는 세계적으로 가장 널리 재배되는 베리류 작물이다. 품종 딸기는 8배체 조상인 F. virginiana (Mill.) 및 F. chiloensis (Mill.) 사이에서의 교배로부터 기원하는 이질8배체(2n = 8x = 56) 종들이다. 한국에서의 딸기 생산량은 농림축산식품부의 통계에 따르면 2010년에 십억 달러 이상이다. 과일의 품질, 강도, 향, 기온적응성 및 병충해 저항성(탄저병, 백분병, 시들음병)은 딸기 육종 프로그램에서 중요한 특징들이다. 새로운 딸기 품종을 육종하는 것은 계통의 선택, 우수한 유전형질의 선택 등 다양한 것들이 요구되며, 이러한 형질을 선택하였다 하더라도 유전자형이 8배체의 이형접합형이라 육종이 까다롭다. 딸기는 주로 러너를 통해 클론적으로 증식하고 있으므로 특허된 품종의 잘못된 또는 불법적인 증식이 문제가 되고 있다. 따라서, 품종의 동정 및 구별은 육종가의 권리를 보호할 뿐 아니라 육종 프로그램에서 품종을 선택하는데 있어 매우 중요하다.Strawberry (Fragaria × ananassa Duch.) is the world's most widely cultivated berry crop. Varieties Strawberries are heterooctaploid (2n = 8x = 56) species originating from a cross between the octaploid ancestors F. virginiana (Mill.) and F. chiloensis (Mill.). Strawberry production in Korea was more than one billion dollars in 2010, according to the statistics of the Ministry of Agriculture, Food and Rural Affairs. Fruit quality, strength, aroma, temperature adaptability and pest resistance (anthrax, powdery mildew, wilt) are important characteristics of strawberry breeding programs. Breeding new strawberry varieties requires various things such as line selection and selection of excellent genetic traits, and even if these traits are selected, breeding is difficult because the genotype is heterozygous for octaploid. Strawberries are mainly propagated clonal through runners, so the wrong or illegal propagation of patented varieties is a problem. Therefore, identification and differentiation of breeds is very important in the selection of breeds in breeding programs as well as in protecting the rights of breeders.
딸기 품종의 동정은 최초로 아이소자임과 같은 생화학적 마커들을 사용하여 이루어졌다. 최근에는 무작위 증폭 다형 DNA(RAPD), 증폭된 단편길이 다형성(AFLP) 및 내부 단순 반복 염기 서열(inter-simple sequence repeat, ISSR) 마커들과 같은 DNA 마커들이 유전자형을 구별하기 위해 개발되었다. Fragaria sequence 정보의 축적은 단순 서열 반복(SSR) 및 cleaved amplified polymorphic sequence (CAPS)과 같은 더욱 정확한 공동우성(codominant) 마커들을 개발하는데 크게 기여하였다. 이러한 분자적 마커들은 유전적 유사성을 축적하고, 유전적 다양성을 모니터링하고, 연결매핑(linkage mapping), 품종을 식별하는데 사용된다. 최근 몇몇 그룹에서 SSR 마커를 딸기 품종 식별에 많이 사용하고 있다. SSR 마커들은 품종내에 다형성이 다수 존재하고, 전체 게놈에 걸쳐서 분포되어있다. 그러나 이러한 유형의 마커를 사용하는 데에는 딸기의 8배체 특성, allele binning 및 automation과 관련된 단점들이 있다. 또한 SSR 마커들은 DNA 단편의 다양한 다형성으로 인해 구분이 쉽지 않은 경우가 다수 존재한다.Strawberry varieties were first identified using biochemical markers such as isozyme. Recently, DNA markers such as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and inter-simple sequence repeat (ISSR) markers have been developed to discriminate genotypes. Accumulation of Fragaria sequence information has greatly contributed to the development of more accurate codominant markers such as simple sequence repeats (SSR) and cleaved amplified polymorphic sequences (CAPS). These molecular markers are used to accumulate genetic similarity, monitor genetic diversity, linkage mapping, and identify cultivars. Recently, several groups have been using SSR markers to identify strawberry varieties. SSR markers have many polymorphisms within cultivars and are distributed throughout the genome. However, the use of this type of marker has disadvantages related to strawberry octaploid characterization, allele binning and automation. In addition, there are many cases in which it is difficult to distinguish SSR markers due to various polymorphisms of DNA fragments.
최근 몇 년간 선호되는 DNA 마커 유형은 단일 염기 다형성(SNP)에 기초한 것이다. 최근 F. vesca 'Hawaii 4'의 2배체 게놈 서열이 완성되었으며, 이러한 게놈 정보는 SNP를 포함한 마커 개발에 유용한 정보로 이용할 수 있다. SNP는 bi-allelic이고, 게놈에서 매우 자주 나타나고, 가장 중요하게는 멀티플렉스하고 자동화할 수 있다는 점에서 다른 분자 마커에 비해 유리하다.The preferred type of DNA markers in recent years are those based on single nucleotide polymorphisms (SNPs). Recently, the diploid genome sequence of F. vesca 'Hawaii 4' was completed, and this genomic information can be used as useful information for the development of markers including SNPs. SNPs have an advantage over other molecular markers in that they are bi-allelic, appear very frequently in the genome, and most importantly, can be multiplexed and automated.
SNP를 사용한 품종의 동정은 딸기, 멜론, 와인포도에서 최근 보고되고 있다. 딸기와 같은 8배체 종에서의 고밀도 SNP 마커 플랫폼의 개발은 부분상동염색체 서열 변이(HSVs)의 존재 때문에 매우 복잡하고 어렵다.Identification of cultivars using SNP has recently been reported in strawberries, melons, and wine grapes. The development of high-density SNP marker platforms in octaploid species such as strawberry is very complex and difficult due to the presence of HSVs.
이러한 배경에서, 본 발명자들은 더욱 정확하고 효율적인 딸기 품종 식별을 위하여 예의 연구한 결과 본 발명을 완성하게 되었다. Against this background, the present inventors have completed the present invention as a result of intensive research for more accurate and efficient identification of strawberry varieties.
본 발명의 일 목적은 서열번호 1 내지 96의 염기서열에서 각 염기서열의 일 말단의 200번째 염기에 위치한 단일염기다형성 (single nucleotide polymorphism, SNP) 전부를 마커로 포함하는 딸기 순도검정 및 품종 판별용 마커 세트를 제공하는 것이다.One object of the present invention is for strawberry purity test and variety identification including all single nucleotide polymorphism (SNP) located at the 200th base of one end of each base sequence in the base sequence of SEQ ID NOs: 1 to 96 as a marker To provide a set of markers.
본 발명의 다른 일 목적은 상기 마커 세트를 포함하는, 딸기 순도검정 및 품종 판별용 키트를 제공하는 것이다.Another object of the present invention is to provide a kit for strawberry purity test and variety identification, including the marker set.
본 발명의 다른 일 목적은 딸기로부터 유래된 게놈 DNA를 주형으로 하고 상기 딸기 순도검정 및 품종 판별용 마커 세트를 사용하여 핵산을 증폭하는 단계; 및 얻어진 증폭 산물로부터 상기 딸기의 순도 및 품종을 결정하는 단계를 포함하는 딸기 순도검정 및 품종 판별 방법을 제공하는 것이다.Another object of the present invention is to amplify the nucleic acid using the genomic DNA derived from strawberry as a template and the marker set for the strawberry purity test and variety discrimination; and determining the purity and variety of the strawberry from the obtained amplification product.
본 발명의 일 양상은 서열번호 1 내지 96의 염기서열에서 각 염기서열의 일 말단의 200번째 염기에 위치한 단일염기다형성 (single nucleotide polymorphism, SNP) 전부를 마커로 포함하는 딸기 순도검정 및 품종 판별용 마커 세트를 제공한다.One aspect of the present invention is for strawberry purity test and variety identification including all single nucleotide polymorphism (SNP) located at the 200th base of one end of each base sequence in the base sequence of SEQ ID NOs: 1 to 96 as a marker A set of markers is provided.
상기 서열번호 1 내지 96의 염기서열은 다음과 같다:The nucleotide sequences of SEQ ID NOs: 1 to 96 are as follows:
96
상기 SNP (단일염기다형성, single nucleotide polymorphism)은 DNA에서 한 개의 뉴클레오티드의 삽입, 소실, 또상기 서열번호 1 내지 96의 염기서열은 단일염기다형성 (single nucleotide polymorphism, SNP)일 수 있고, 상기 단일염기다형성 (single nucleotide polymorphism, SNP)은 DNA에서 한 개의 뉴클레오티드의 삽입, 소실, 또는 치환이 일어나는 것을 말한다. ‘다형성'이라는 용어는 군집 내에서 변하는 유전자의 서열에서의 배치를 지칭한다. 다형성은 상이한 "대립유전자"로 구성된다. "대립유전자"는 한 쌍의 상동염색 체에서 서로 대응하는 부위, 즉 상동하는 유전자자리에 위치하는 대립형질에 대응하는 유전자를 말하며, 같은 염색체 위치 (same chromosomallocus)를 점유하는 한 유전자의 둘 또는 그 이상의 선택적인 형태 (alternative forms) 중 하나를 뜻한다. 이러한 다형성의 배치는 유전자에서의 그의 위치 및 그에서 발견되는 상이한 아미노산 또는 염기에 의해 확인될 수 있다. 이러한 아미노산 변이는 2개의 상이한 대립유전자인, 2개의 가능한 변이체 염기, C 및 T의 결과이다. 유전자형은 2개의 다른 별개의 대립유전자로 구성되기 때문에, 여러 가능한 변이체 중 임의의 변이체가 어느 한 개체에서 관찰될 수 있다 (예를 들어, CC (A로 표시), CT (H로 표시) 또는 TT (B로 표시)). 개개의 다형성은 또한 당업자에게 공지되어 있다.The SNP (single nucleotide polymorphism) is the insertion or deletion of one nucleotide in DNA, and the nucleotide sequence of SEQ ID NOs: 1 to 96 may be a single nucleotide polymorphism (SNP), and the single nucleotide Single nucleotide polymorphism (SNP) refers to the insertion, deletion, or substitution of one nucleotide in DNA. The term 'polymorphism' refers to an arrangement in the sequence of a gene that varies within a population. Polymorphisms consist of different “alleles”. "Allele" means a gene corresponding to an allele located at a region corresponding to each other in a pair of homologous chromosomes, i.e., at a homologous locus, and refers to two or more of a gene occupying the same chromosomallocus. It means one of the above alternative forms. The placement of such polymorphisms can be identified by their position in the gene and the different amino acids or bases found therein. These amino acid variations are the result of two different alleles, two possible variant bases, C and T. Because the genotype consists of two different distinct alleles, any of several possible variants can be observed in any one individual (e.g., CC (denoted A), CT (denoted H) or TT (denoted by B)). Individual polymorphisms are also known to those skilled in the art.
구체적으로 상기 단일염기다형성은 각 서열번호 1 내지 96 염기서열의 일 말단으로부터 200번째 염기에 위치하는 것일 수 있다. 더욱 구체적으로, 각 염기서열의 5’(전단) 또는 3‘(말단) 위치에서부터 200번째 염기에 위치하는 것일 수 있고, 가장 구체적으로는 서열번호 38 및 39는 단일염기다형성이 각 염기서열의 전단으로부터 200번째 위치할 수 있고, 서열번호 10, 26, 47, 57, 60, 61, 62, 74, 83, 84 및 92는 단일염기다형성이 각 염기서열의 말단으로부터 200번째 위치할 수 있으며, 나머지 서열번호들의 염기서열의 경우 400개의 염기서열을 가지고 있어 단일염기다형성이 전단 또는 말단의 위치에서부터 200 번째 염기에 위치하는 것일 수 있다. Specifically, the single nucleotide polymorphism may be located at the 200th base from one end of each SEQ ID NO: 1 to 96 nucleotide sequence. More specifically, it may be located at the 200th base from the 5' (front end) or 3' (terminal) position of each nucleotide sequence, and most specifically, SEQ ID NOs: 38 and 39 show that single nucleotide polymorphisms are the front end of each nucleotide sequence. may be located at the 200th position from, and in SEQ ID NOs: 10, 26, 47, 57, 60, 61, 62, 74, 83, 84 and 92, the single nucleotide polymorphism may be located at the 200th position from the end of each nucleotide sequence, and the remaining In the case of the nucleotide sequence of SEQ ID NOs, it has 400 nucleotide sequences, so single nucleotide polymorphism may be located at the 200 th base from the front end or terminal position.
상기 마커 세트는 딸기 순도 검정 및 품종을 식별하기 위해 이용될 수 있고, 구체적으로, 구별가능한 딸기 품종은 설향, 챈들러, 사치노카, 도요노카 및 베니훗베일 수 있다. The marker set may be used to assay strawberry purity and identify varieties, and specifically, distinguishable strawberry varieties may be Seolhyang, Chandler, Sachinoka, Toyonoka, and Benihutbe.
상기 마커 세트의 프라이머는 포스포로티오에이트(phosphorothioate), 알킬포스포로티오에이트와 같은 뉴클레오티드 유사체(analogue), 펩티드 핵산(peptide nucleic acid) 또는 삽입 물질(intercalating agent)을 포함할 수 있다. The primer of the marker set may include a nucleotide analogue such as phosphorothioate or alkyl phosphorothioate, a peptide nucleic acid, or an intercalating agent.
본 발명의 일 구체예로서 상기 마커는 검출 가능한 물질로 표지 되어 있을 수 있고, 검출 가능한 물질의 예시로서 형광, 인광 또는 방사성을 발하는 표지물질일 수 있다. 상기 표지 물질은 상기 마커의 5' 말단에 표지될 수 있다. In one embodiment of the present invention, the marker may be labeled with a detectable material, and may be a label that emits fluorescence, phosphorescence, or radioactivity as an example of a detectable material. The labeling material may be labeled at the 5' end of the marker.
상기 마커는 공지된 방법을 사용하여 화학적으로 합성할 수 있다. 이러한 서열은 또한 당해 분야에 공지된 많은 수단을 이용하여 변형시킬 수 있다. 이러한 변형의 비-제한적인 예로는 메틸화, 캡화, 천연 뉴클레오타이드 하나 이상의 동족체로의 치환, 및 뉴클레오타이드 간의 변형, 예를 들면, 하전되지 않은 연결체(예: 메틸 포스포네이트, 포스포트리에스테르, 포스포로아미데이트, 카바메이트 등) 또는 하전된 연결체(예: 포스포로티오에이트, 포스포로디티 오에이트 등)로의 변형이 있다.The marker can be chemically synthesized using a known method. Such sequences may also be modified using a number of means known in the art. Non-limiting examples of such modifications include methylation, encapsulation, substitution with one or more homologues of natural nucleotides, and modifications between nucleotides, such as uncharged linkages such as methyl phosphonates, phosphotriesters, phosphoro amidates, carbamates, etc.) or charged linkages (eg phosphorothioates, phosphorodithioates, etc.).
본 발명의 다른 일 양상은 상기 조성물을 포함하는, 딸기 순도검정 및 품종 판별용 키트를 제공한다.Another aspect of the present invention provides a kit for strawberry purity test and variety identification, comprising the composition.
상기 키트는 딸기 순도검정 및 품종 판별을 위한 공지의 물질을 더 포함할 수 있고, 구체적으로, DNA 중합 효소, dNTP 및 PCR 완충용액을 더 포함할 수 있다. 또한, PCR 반응 또는 증폭 산물의 확인에 필요한 구성 성분이 상기 키트에 추가로 포함될 수 있다. 상기 PCR 완충용액은 KCl, Tris-HCl 및 MgCl2를 함유할 수 있다. 상기 키트는 안내서를 포함할 수 있다. 안내서는 키트 사용법, 예를 들면, PCR 완충액 제조 방법, 제시되는 반응 조건 등을 설명하는 인쇄물이다. 안내서는 팜플렛 또는 전단지 형태의 안내 책자, 키트에 부착된 라벨, 및 키트를 포함하는 패키지의 표면상에 설명을 포함한다. 또한, 안내서는 인터넷과 같이 전기 매체를 통해 공개되거나 제공되는 정보를 포함한다.The kit may further include known materials for strawberry purity test and variety identification, and specifically, DNA polymerase, dNTP and PCR buffer may be further included. In addition, components necessary for PCR reaction or identification of amplification products may be additionally included in the kit. The PCR buffer may contain KCl, Tris-HCl and MgCl2. The kit may include instructions. A handbook is a printout explaining how to use the kit, eg, how to prepare a PCR buffer, and suggested reaction conditions. Instructions include a brochure in the form of a pamphlet or leaflet, a label affixed to the kit, and instructions on the surface of the package containing the kit. In addition, the guide includes information published or provided through an electronic medium such as the Internet.
상기 마이크로어레이(microarray)는 기판 표면의 구분된 영역에 상기 단일염기다형성을 검출할 수 있는 폴리뉴클레오티드, 예를 들면 프로브가 높은 밀도로 고정화되어 있는 것일 수 있다.The microarray may be a polynucleotide capable of detecting the single nucleotide polymorphism, for example, a probe immobilized at a high density in a divided region on the surface of a substrate.
상기 플루이다임 SNP 분석용 키트는 플루이다임 시스템용 프라이머를 포함할 수 있고, 구체적으로 STA, LSP, 및 ASP 1&2 프라이머를 포함할 수 있다. 플루이다임 SNP 분석용 키트는 DNA 중합효소, dNTP 혼합물 및 PCR 완충 용 액을 더 포함하는 것일 수 있다. DNA 중합효소 및 PCR 완충 용액은 상기 PCR 키트에서 설명한 내용을 참조하여 이해될 수 있다.The Fluidime SNP analysis kit may include primers for the Fluidime system, and specifically may include STA, LSP, and ASP 1&2 primers. The kit for Fluidime SNP analysis may further include a DNA polymerase, a dNTP mixture, and a PCR buffer solution. DNA polymerase and PCR buffer solution may be understood with reference to the description of the PCR kit.
본 발명의 다른 일 양상은 딸기로부터 유래된 게놈 DNA를 주형으로 하고 상기 딸기 순도검정 및 품종 판별용 마커 세트를 사용하여 핵산을 증폭하는 단계; 및 상기 증폭 산물로부터 상기 딸기의 순도 및 품종을 결정하는 단계를 포함하는 딸기 순도검정 및 품종 판별 방법을 제공한다.Another aspect of the present invention comprises the steps of using genomic DNA derived from strawberry as a template and amplifying the nucleic acid using the marker set for strawberry purity test and variety discrimination; and determining the purity and variety of the strawberry from the amplification product.
상기 게놈 DNA는 상기 딸기의 잎, 줄기, 과실, 또는 그의 조합으로부터 유래된 것일 수 있다. 구체적으로 본 발명의 방법을 위해 본 발명자들은 여러 딸기 품종의 잎 조직에서부터 게놈 DNA를 추출하였다. 상기 게놈 DNA는 식물체로부터 DNA를 채취하는 통상적인 방법을 이용하여 수득 될 수 있다. 상기 게놈 DNA는 예를 들면, 페놀 추출 방법을 이용하여 수득 될 수 있다.The genomic DNA may be derived from leaves, stems, fruits of the strawberry, or a combination thereof. Specifically, for the method of the present invention, the present inventors extracted genomic DNA from leaf tissues of several strawberry varieties. The genomic DNA can be obtained using a conventional method for collecting DNA from a plant. The genomic DNA may be obtained using, for example, a phenol extraction method.
상기 핵산을 증폭하는 단계는 딸기로부터 유래된 게놈 DNA를 주형으로 하고 상기 딸기 순도검정 및 품종 판별용 마커 세트를 사용하여 증폭하는 단계로서, 상기 핵산의 증폭은 PCR을 이용하여 수행될 수 있다. PCR 방법에 대해서는 당업계에 잘 알려져 있으며, 상업적으로 이용가능한 키트를 이용할 수 있다. PCR 반응은 당업계에 PCR 반응에 필요한 것으로 알려진 여러 성분을 포함하는 PCR 반응액을 이용하여 수행될 수 있다. 상기 PCR 반응액은 예를 들면, 분석하고자 하는 딸기로부터 유래된 게놈 DNA, 본 발명의 SNP 프라이머 세트, DNA 중합 효소(예를 들면, Taq polymerase), dNTP 혼합물, PCR 완충용액 및 물을 포함하는 것일 수 있다. The step of amplifying the nucleic acid is a step of amplifying the strawberry-derived genomic DNA as a template using the marker set for the strawberry purity test and variety discrimination, and the amplification of the nucleic acid may be performed using PCR. PCR methods are well known in the art and commercially available kits are available. The PCR reaction may be performed using a PCR reaction solution containing various components known in the art for a PCR reaction. The PCR reaction solution includes, for example, genomic DNA derived from strawberry to be analyzed, the SNP primer set of the present invention, a DNA polymerase (eg, Taq polymerase), a dNTP mixture, a PCR buffer solution, and water. can
본 발명의 일 구체예로서, 상기 핵산을 증폭하는 단계에서 얻어진 증폭 산물을 DNA 칩, 겔 전기영동, 방사성 측정, 형광 측정 또는 인광 측정을 통해 검출하는 단계를 포함할 수 있다. 겔 전기영동을 통해 검출할 경우, 증폭 산물의 크기에 따라 아가로스 겔 전기영동 또는 아크릴아미드 겔 전기영동을 이용할 수 있다. 형광 측정을 통해 검출할 경우, 형광 물질로 표지된 본 발명의 프라이머 세트를 이용하여 PCR을 수행한 후 형광 측정기를 이용하여 형광을 측정할 수 있다. In one embodiment of the present invention, the method may include detecting the amplification product obtained in the step of amplifying the nucleic acid through a DNA chip, gel electrophoresis, radiometric measurement, fluorescence measurement, or phosphorescence measurement. In the case of detection through gel electrophoresis, agarose gel electrophoresis or acrylamide gel electrophoresis can be used depending on the size of the amplification product. In the case of detection through fluorescence measurement, after PCR is performed using the primer set of the present invention labeled with a fluorescent material, fluorescence may be measured using a fluorescence meter.
상기 품종을 결정하는 단계는 증폭 산물로부터 상기 딸기의 순도 및 품종을 결정하는 단계이다. The step of determining the variety is a step of determining the purity and variety of the strawberry from the amplification product.
본 발명의 일 구체예에서, 상기 증폭 산물로부터 상기 딸기의 순도 및 품종을 결정하는 단계는, 얻어진 증폭 산물을, 제1항에 따른 딸기 순도검정 및 품종 판별용 마커 세트에 의해 공지된 딸기 품종의 핵산을 증폭한 결과와 비교함으로써 수행되는 것일 수 있다.In one embodiment of the present invention, the step of determining the purity and variety of the strawberry from the amplification product comprises using the obtained amplification product of a strawberry variety known by the strawberry purity test and variety discrimination marker set according to claim 1 . It may be performed by comparing the result of amplifying the nucleic acid.
구체적으로, 상기 증폭된 산물로부터 딸기 품종을 결정하는 단계는 자동 염기서열 분석기를 이용하여 증폭 산물 (밴드)의 양을 컴퓨터 프로그램에 의해 확인함으로써 수행되는 것일 수 있다.Specifically, the step of determining the strawberry variety from the amplified product may be performed by using an automatic sequencing analyzer to check the amount of the amplified product (band) by a computer program.
본 발명의 딸기 순도검정 및 품종 판별용 마커 세트, 이를 포함하는 키트 및 이를 이용한 딸기 순도검정 및 품종 판별 방법은 딸기의 순도검정 및 품종 판별을 신속하고 정확하게 판단할 수 있다.The marker set for strawberry purity test and variety discrimination of the present invention, a kit including the same, and a strawberry purity test and variety discrimination method using the same can quickly and accurately determine the purity test and variety discrimination of strawberries.
도 1은 SNP 마커 (FAN-SCF1625) 활용한 유전자형 분석 결과를 나타내는 그래프이다.
도 2는 딸기의 5 품종의 8 고정계통에 대한 유전자형 분석 결과를 나타내는 그림이다.
도 3은 본 발명의 마커 세트를 이용하여 설향, 챈들러, 사키노카, 도요노카 및 베이훗베 5 품종의 판별한 결과를 나타내는 도이다.1 is a graph showing the results of genotyping using a SNP marker (FAN-SCF1625).
2 is a diagram showing the results of genotyping for 8 fixed strains of 5 varieties of strawberry.
3 is a diagram showing the results of discrimination of 5 varieties Seolhyang, Chandler, Sakinoka, Toyonoka and Beihutbe using the marker set of the present invention.
이하 하나 이상의 구체예를 실시예를 통하여 보다 상세하게 설명한다. 그러나, 이들 실시예는 하나 이상의 구체예를 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, one or more specific examples will be described in more detail through examples. However, these examples are for illustrative purposes of one or more embodiments, and the scope of the present invention is not limited to these examples.
실시예 1: 재료 및 방법Example 1: Materials and Methods
1-1. 시험 재료1-1. test material
딸기 품종 설향(대조구), 챈들러, 사치노카, 도요노카 및 베니훗베 5품종과, 챈들러의 S9세대 S27-3, S27-10, 사치노카의 S10세대 105(P209), P190, 도요노카 S9세대 9-1, 50(9-2), 50-2(9-3), 베니훗베의 S9세대 8-9 총 8개의 고정계통을 활용하였다.Strawberry varieties Seolhyang (control), Chandler, Sachinoka, Toyonoka and Benifutbe 5 varieties, Chandler's S 9th generation S27-3, S27-10, Sachinoka's S 10th generation 105 (P209), P190, Toyonoka S A total of 8 fixed lines were used: 9th generation 9-1, 50(9-2), 50-2(9-3), and S 9th generation 8-9 of Benihubbe.
1-2. 딸기 ‘설향’ 및 ‘생가생가나’ 염기서열 분석 및 품종 간 SNP 탐색 및 선발1-2. Strawberry ‘Seolhyang’ and ‘Saenggasangana’ base sequence analysis and SNP search and selection between varieties
CTAB 방법을 이용하여 고순도의 DNA를 분리하였고, DNA 라이브러리를 구축하여 해당 라이브러리를 Illumina Hiseq 2000 장비를 이용하여 2 품종 (설향 및 생가생가나)의 염기서열 분석을 실시하였다.High-purity DNA was isolated using the CTAB method, and a DNA library was constructed and sequencing of the two varieties (Seolhyang and Saenggasangana) was performed using the Illumina Hiseq 2000 equipment.
SAMtools (0.1.16) 프로그램을 사용하여 raw SNP을 detection하고 consensus sequence를 추출하였다.Using the SAMtools (0.1.16) program, raw SNPs were detected and consensus sequences were extracted.
분석대상 간의 SNP 비교분석을 수행하기 위해 샘플간 통한 SNP matrix를 작성하였으며, 각 샘플을 표준유전체와 비교하여 얻은 raw SNP position을 후보로 하여 리스트를 구축하였다.In order to perform SNP comparison analysis between analytes, a SNP matrix through samples was prepared, and a list was constructed using raw SNP positions obtained by comparing each sample with a standard genome as candidates.
최종적으로 SNP filtering을 통해 선발조건을 만족하는 SNP를 선발하였다. Finally, SNPs satisfying the selection conditions were selected through SNP filtering.
1-3. HRM (high resolution melting) 분석에 적합한 SNP primer 디자인1-3. SNP primer design suitable for HRM (high resolution melting) analysis
선발된 SNP는 비교 샘플 간의 다형성 SNP좌를 target으로 하여 HRM용 SNP primer를 개발하였다. primer는 Primer3(v.2.3.5) 프로그램을 사용하여 디자인하였으며, in silico PCR을 수행하여 게놈상에 한 번 관찰되는 primer를 선발하였다. For the selected SNP, a SNP primer for HRM was developed by targeting the polymorphic SNP locus between comparative samples. The primer was designed using the Primer3 (v.2.3.5) program, and in silico PCR was performed to select a primer that was observed once on the genome.
실시예 2: 연구 결과Example 2: Study Results
2-1.2-1. 딸기 ‘설향’ 및‘생가생가나’의 유전체 염기서열 분석 및 SNP 탐색 및 선발Genome sequencing and SNP search and selection of strawberries ‘Seolhyang’ and ‘Saenggasangana’
신초에서 DNA를 추출하여 DNA 라이브러리를 구축하여 해당 염기서열을 Illumina Hiseq 2000을 활용하여 분석하였고, 두 품종에 대한 총 553,848,668 reads를 얻었다. 또한 read mapping을 수행하였으며, ‘설향’ 및 ‘생가생가나’ 각각 76.0(약 530 Mbp), 76.5(약 534 Mbp) %의 overall read mapping rate을 보였다. DNA library was constructed by extracting DNA from shoots, and the corresponding nucleotide sequence was analyzed using Illumina Hiseq 2000, and a total of 553,848,668 reads were obtained for both varieties. Also, read mapping was performed, and the overall read mapping rates of ‘Seolhyang’ and ‘Saenggasangana’ were 76.0 (about 530 Mbp) and 76.5 (about 534 Mbp) %, respectively.
각 샘플의 raw SNP를 이용하여 딸기 품종 간의 통합 SNP matrix를 작성하였다. 필터 기준을 통과한 SNP들은 표준유전체에 mapping된 샘플의 read가 90% 이상으로 동일한 SNP type을 나타내는 경우 homozygous, 40~60%만 동일한 SNP type을 나타내는 경우 heterozygous, homozygous/heterozygousfh type으로 구분할 수 없는 경우 etc.로 구분하였다. SNP는 유전체상의 위치를 확인하기 위해 intergenic- 과 genic (exon, CDS, intron)- region으로 구분하여 분류하였다. An integrated SNP matrix between strawberry varieties was prepared using the raw SNPs of each sample. SNPs that have passed the filter criteria are homozygous when 90% or more of the sample mapped to the standard genome shows the same SNP type, and when only 40 to 60% of the sample shows the same SNP type, heterozygous, homozygous/heterozygousfh type. etc. SNPs were classified into intergenic- and genic (exon, CDS, intron)- regions to confirm their location on the genome.
딸기 2품종 union SNP matrix에서 동일 SNP좌를 비교하여 두 품종 간 서로 다른 SNP인 경우(polymorphic SNP)와 두 품종 간 서로 동일한 SNP인 경우(non-polymorphic SNP)을 선발하였다. 총 334,319개의 polymorphic SNP와 87,161개의 non-polymorphic SNP가 선발되었으며, 이 중 polymorphic SNP을 target으로 하여 HRM용 SNP primer 총 3,144개을 선발하였다.By comparing the same SNP locus in the union SNP matrix of two strawberry varieties, different SNPs between the two varieties (polymorphic SNP) and those with the same SNP between the two varieties (non-polymorphic SNP) were selected. A total of 334,319 polymorphic SNPs and 87,161 non-polymorphic SNPs were selected, and a total of 3,144 SNP primers for HRM were selected by targeting polymorphic SNPs.
선발한 3,144개 중 1,857개의 SNP primer를 HRM 분석하였으며, 실제로 다형성을 나타내지 않거나 분석하기 모호한 1,057개의 SNP를 제외하여 최종적으로 800개의 SNP 마커세트를 선발하였다. 1,857 SNP primers out of 3,144 selected were subjected to HRM analysis, and 800 SNP marker sets were finally selected, excluding 1,057 SNPs that did not actually show polymorphism or were ambiguous to analyze.
2-2. 딸기 품종 및 S2-2. Strawberry varieties and S 99 ~S~S 1010 세대에 SNP 마커세트를 적용하여 효용성 검정Efficacy test by applying a set of SNP markers to generations
개발한 800개의 SNP 프라이머를 딸기 5품종 및 8개의 고정계통에서 다형성이 뚜렷한 SNP priemer를 선발하였다 (도 1). 유전자형 분석 결과 총 96개의 HRM용 SNP primer를 최종 선발하였다(도 2, 표 1). With the developed 800 SNP primers, SNP primers with clear polymorphism were selected from 5 strawberry varieties and 8 fixed strains (FIG. 1). As a result of genotyping, a total of 96 SNP primers for HRM were finally selected (Fig. 2, Table 1).
개발된 96개의 딸기 마커세트로 품종 및 고정계통간의 다형성을 보이는 SNP를 선발하고, 이를 통해 고정도 및 품종을 판별할 수 있음을 확인하였다(도 2, 표 1). SNPs showing polymorphism between varieties and fixed lines were selected using the developed 96 strawberry marker sets, and it was confirmed that high precision and varieties could be discriminated through this (Fig. 2, Table 1).
분석에 사용된 품종 및 고정계통들의 유전적 다양성을 분석하기 위해 유전적 계통수(Phylogenetic tree, Dendrogram) 분석을 실시하였다. 계통 발생 분석은 인접 결합 방법(neighbor-joining method)을 사용하여 Darwin6 소프트웨어(http://darwin.cirad.fr/)에서 수행하였다. 계통도의 구조는 비가중 인접 결합 방법(unweighted neighbor-joining)에 기반하였고, 부트스트랩(bootstraps)은 1,000 반복(replicates)으로 결정하였다. In order to analyze the genetic diversity of the cultivars and fixed lines used in the analysis, a phylogenetic tree (Dendrogram) analysis was performed. Phylogenetic analysis was performed in Darwin6 software (http://darwin.cirad.fr/) using the neighbor-joining method. The structure of the phylogenetic tree was based on an unweighted neighbor-joining method, and bootstraps were determined to be 1,000 replicates.
설향, 챈들러, 사치노카, 도요노카 및 베니훗베 5품종의 경우 품종 판별이 가능했으며, 챈들러 유래의 고정계통인 S27-3과 S27-10는 유전적으로 동일한 양상을 보였다. 또한 도요노카 유리의 고정계통인 9-1와 50-2(9-2)의 경우도 유전적으로 동일한 양상을 보였다(도 3). 같은 원품종 유래에도 불구하고 유전적으로 동일한 양상을 보이지 않는 고정계통의 경우는 초기에 세대 분리가 발생하였거나 계속된 세대진전으로 인해 고정되면서 원품종과 유래계통간에 유전적으로 멀어진 것으로 보여 진다.In the case of 5 varieties of Seolhyang, Chandler, Sachinoka, Toyonoka, and Benihutbe, it was possible to identify the varieties, and S27-3 and S27-10, which are fixed lines derived from Chandler, showed the same genetic pattern. Also, the fixed strains of Toyonoka Yuri, 9-1 and 50-2 (9-2), showed the same genetic pattern (FIG. 3). In the case of a fixed lineage that does not show the same genetic pattern despite the origin of the same original variety, it appears that the generational segregation occurred at the beginning or it was fixed due to continued generational progress, resulting in a genetic distance between the original variety and the derived lineage.
딸기 5품종 및 8개의 고정계통의 유전적 고정도를 분석하였으며, 대조군인 설향의 경우 83.3 %의 이형접합성(heterozygosity)을 보였으며, 다른 품종의 경우 38.5 ~ 59.4 %의 이형접합성을 보였다. 고정 계통의 경우 품종보다 낮은 이형접합성을 보였으며, 반대로 높은 동형접항성을 보였다 (표 2).The genetic fixity of 5 strawberry varieties and 8 fixed strains was analyzed. Seolhyang, a control group, showed 83.3% heterozygosity, and other varieties showed 38.5-59.4% heterozygosity. Fixed strains showed lower heterozygosity than cultivars and, conversely, high homozygosity (Table 2).
분석 결과 96개의 SNP 마커로 딸기 품종을 모두 판별할 수 있을 것으로 나타났으며 (도 3), 또한 품종 유래의 고정계통의 유전자형을 분석하여 고정도를 확인할 수 있다 (표 2).As a result of the analysis, it was shown that all strawberry varieties could be discriminated by 96 SNP markers (FIG. 3), and the high accuracy could be confirmed by analyzing the genotype of the fixed line derived from the variety (Table 2).
상기한 결과들은 다음과 같이 요약할 수 있다: The above results can be summarized as follows:
국내 주요 품종‘설향’과 이와 유연관계가 먼‘생가생가나’의 어린잎에서 DNA를 추출하여 whole genome resequencing을 진행하였다. 해당 염기서열 분석은 Illumina Hiseq 2000을 활용하였고, 분석된 염기서열 정보를 활용하여 대량의 유용 SNP 3,144개를 확보하였으며, 이 중 High resolution melting (HRM) 분석용 1,857개의 SNP 프라이머 세트를 개발하였다.Whole genome resequencing was performed by extracting DNA from young leaves of ‘Seolhyang’, a major domestic cultivar, and ‘Saenggasangana’, which has a distant relationship with it. Illumina Hiseq 2000 was used for the nucleotide sequence analysis, and a large amount of 3,144 useful SNPs were secured using the analyzed nucleotide sequence information, of which 1,857 SNP primer sets were developed for high resolution melting (HRM) analysis.
딸기는 이질배수성 8배체 작물로 유전체 내 상동성 부위가 많아 품종판별 마커 분석에 어려움이 있다. 개발된 마커세트는 이러한 문제점을 해결하고자 sub-genome 상에 1 copy로 존재하는 SNP 프라이머를 선발하였다. 개발된 마커세트는 딸기 순도검정 및 품종 판별이 가능하며, 또한 유전적 거리에 기반한 근연관계 분석 등이 가능하다. Strawberry is a heterogeneous octaploid crop, and there are many homologous regions in the genome, making it difficult to analyze cultivar markers. The developed marker set selected SNP primers present in 1 copy on the sub-genome to solve this problem. The developed marker set enables strawberry purity test and variety discrimination, and also enables kinship analysis based on genetic distance.
최종적으로 800개의 SNP primer가 개발되었으며, 이 중 96개의 마커세트를 활용하여 5개의 품종(설향, 챈들러, 사치노카, 도요노카 및 베니훗베)판별과 각각 품종의 S9~S10세대의 8개의 고정계통의 고정도 확인을 통해 마커세트의 효용성을 확인하였다.Finally, 800 SNP primers were developed, of which, using 96 marker sets, 5 varieties (Seolhyang, Chandler, Sachinoka, Toyonoka, and Benihutbe) were identified and 8 S9 ~ S10 generations of each variety were identified. The efficacy of the marker set was confirmed through the confirmation of the high precision of the fixed system.
개발된 SNP 마커세트를 활용하여 딸기 품종을 판별할 수 있고, 고정계통의 순도를 검정하여 고정도를 신속히 판단할 수 있다. 따라서 본 발명은 딸기 품종의 진위성 검정과 품종보호 출원 시 대조 품종의 선정 등 다양한 방면에서 활용될 수 있다.Strawberry varieties can be identified using the developed SNP marker set, and high accuracy can be quickly determined by testing the purity of the fixed line. Therefore, the present invention can be utilized in various fields, such as the verification of the authenticity of strawberry varieties and selection of control varieties when applying for variety protection.
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.So far, with respect to the present invention, the preferred embodiments have been looked at. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.
<110> Republic of Korea(Management: Rural Development Administration) <120> SNP marker set for identifying strawberry cultivars and method for identifying strawberry cultivars using the same <130> RDA-P200044 <160> 96 <170> PatentIn version 3.2 <210> 1 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 1 attttatcat ttgataatat ttgaggtgac tattttaacc cgtgaaacaa ttaatgatat 60 ttttattata tgtacattca aattttagtg ttatttgtcc tactattttc atcaggtcaa 120 aattaagaat atgatatgct tctttttcat ttccttcaac tggtgctgtg taattttgtt 180 acaaagaaaa ggatctagcy atctaggcat ggtatcaaat tgggtcgggt cgaccctcct 240 aaaacacatt gggcaccctg atggtcagcc aacccactaa gtctattaac acataggcca 300 ttgctgggtt gggccgttct atatttcttt aggagtttta tttacgcaaa ctagctagta 360 ttcaagaatc ttgcattcca agcgactttt tttttttttt 400 <210> 2 <211> 294 <212> DNA <213> Fragaria ananassa Duch. <400> 2 caatagattc cacataatat tctcttatca cttgttgact tctccatcac ttctgaacac 60 cgtccgtccg atcctctcac aaagctcaca tcgcgtcaca cccaatccct tctctcaccg 120 acccaatcta aagcaagggt aacatagtca ttgcacccag cccatcagaa tcgacagaac 180 cagaccaaag ctaaaaacay caaccagccg ctgacagcaa ctaaccaacc tctcgaaacg 240 acgtcgtagt tcccaccgag cacgtaaacg acgccgcttc ttttgtcctc attc 294 <210> 3 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 3 tagttctatc ccatgtagac gtcgagctct ctgtcgtatc agagggacaa tgtcataaat 60 gatgatgatg aaactgctgc ttctcctatg tcacaatgtc ataacatttt gtgaagagga 120 tgaagctgca cgtgcaatag ctaaatgttg aaactgttct ctcagactca agcaagcttg 180 gtgcatgaga tttggctccm cttgtaattc gatcgtattt gtaaagtgta ggtcttcttc 240 tattcctatc gatgttggcc gtgatatagg gttcaattag tttctctatg ataattcctt 300 ttcctatatt tgaataaaga gtccgagtct ggacactgca cttgcagaat tggtgcgcgc 360 aatacatgca atgttgaaga ctacacccat atatgatcta 400 <210> 4 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 4 tcgttgtcaa ttaatagaat tacgaacctc accgtatgat ataaatgaga tagggtagtc 60 ttagtccatt ggtttcatga atgcagcaaa atggtcccca aattattgta ccacactctt 120 cctctctttt cctatattcc gtctgtgaca cagtccaatt tttagaccta gaaaactaga 180 tgatgagacc aacacattty agaattttta gtttttcctt tcgaaaattt gtttaggtaa 240 gagaaatgta agaaatttgg ttttaatatc aaagttagcc ctgctcttta acacataata 300 cacgtaaaac cctctgtatg tgtttttatt ggccgaagta agaaaccctc tggttgaaac 360 atttatggtt ccgaatgtgt catcggctag atgtcatggt 400 <210> 5 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 5 ttcaactctc tcaaggaatc caactttcca ataatttttg gaaattcctg tagcccaata 60 caatattgaa gatccaagat ctcaagcttc tccagcgatc caatggaatg aggaatctca 120 accaagctta tacaagctgt taaatggagt tcgattaagt tggggattcc gctcaagtct 180 ggaaattctc ttagagatty acaccgactt aaatccatat attttagatt tggcgaattc 240 tgtgtagcaa gaacaggaag aaaatatcaa ttaaccaacc aaaagcaata ataatttata 300 acattgaaaa taaaaccgag tataatattg aagtacctta cacccgttcc aaagccgagt 360 aatgtgacaa ctgtcagaaa tatgaagctt gacaagctta 400 <210> 6 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 6 atctgaatcg ggtgaagaaa ggaggtcctt gggcttacca acaagctatg atcttgatca 60 atgattacga tggcttttgt gatatcatgg ttgtcccatt ggattttgtg tggatatggg 120 tggaaattga gggacttccg gttgccctga tgacggaggc gacgactaga cttgtgggag 180 agaccattgg taccgtgctr taagtcgaca ccctgggttt gaacagaggt gtggctaggg 240 ttcgtattat tctacttaat aaccctgtaa gatttgaaag gagaattagg gtttcccctg 300 aagatgttat tctggtcaag taagtataag tgactactta gccgatatcg tatttgctgg 360 atgattaatc atggaggaag gtcttgtcca tgtgaagctg 400 <210> 7 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 7 agggtgttaa caagtttaag agtagcaaaa ttaccgatgg tagttatagt gcaaacctca 60 gtctcacaat taatcagaag caagctacaa attttgaaga agatattaga tggctgtttt 120 tggtccatct acagcatgag ctagttgagc taatatgtca atttgagcta gttgtatata 180 gaggtcatca ttaagcatam aactatgacg aagtatgaag tgcatgtctc aacgattaat 240 agcactgatg agagaagaca aagaatgaca attttgaatt ttcaagcata atcaatcgtc 300 cacaaatgct cggattgatt tagaacatca ttcatctaaa aagaaaatag aataatttct 360 agctagagta gtaaacgtta caaaatgcaa aatgcagtag 400 <210> 8 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 8 taggtttgga tgccaacaac actacctatg ttgttgctta tgcaatggtg gaaatggaga 60 acaaggattc atggatttgg tttctatgcc ttttgcagaa ggatctgaag ataactggag 120 agggaaatgg gttcacgtac tttcatatct gataagcaga aggggttgct tccagcatgt 180 gaatatgtcc tcccactagy tgagcacaga ttttgtgtga ggcacttgtg gactaatttt 240 aacaagaagt ttccagggaa agcattgaag gatcaacttt gggcaattgc caaatcaaca 300 acaagggcct attactggaa agaaatgact ttgttgaatc agatggacac agatgcttat 360 gattggataa ctggtaagac tggtcaattc atgtgtgatt 400 <210> 9 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 9 agatcatcgc tcccaagcaa aggcacaaaa ccctaagtgc gcacctctcc aaccaaaccc 60 ttcgaactcc atggcgttcg cccatccaaa gctaagcctc tctccatcat catcaacccc 120 caataagtcc tcgacctcgt cgtcgatgac aaagacccca aacaagtatt ctgcatcttc 180 atccatggtg caggcctcgy ccattgataa agacaccaag tcctttaccg cctcgcttgc 240 gcttgcaagt tcaaaggtgg ttgatctctc tgctggcatc tttgggctca aacgacgtgc 300 tacatacttt tttctggtag ccaagcccct caactttgag ccaccatctc ttgtgaacat 360 gaaaatgttc tgtaatagag tttgaggtat tggagaggaa 400 <210> 10 <211> 278 <212> DNA <213> Fragaria ananassa Duch. <400> 10 tctggtgtga tatttggctt gtattctagt atttgtcctc cccaaaacat ttgattcatg 60 ttatatttat agtcgcartc ccatcgatta ttctgactaa tacacattat gcgcgcttca 120 ttcacaattc atacacgaat ggatcctcaa taccacattc tggacatcat gggaaagtgt 180 ctttagtttc ctcttgttga ctagtacttc tacttcactt taaccacaac ttgttctaac 240 cgtaactcta tttgcccacg acgcaatttg actgaagc 278 <210> 11 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 11 gttgatatag atagtttctg atagataatt gcttcacttg ttgaatgcat cacacatcat 60 gatatgatta taacctgaat atcctattta tggcaaagtt tattataact tggacctacc 120 cctacccctt tctatccctt cacagctaca tgaagcaaat tgttcgtaat ccttcctgct 180 aatataattt caacacaacr tcaatttttt atggtttcgc tggccattcc actaacctgt 240 caggtttatg tatagaacat tgcaaacaag tttttagaga tcagaaagta tatttaaagg 300 agtttaaatg gtttaaacat tataattatt aaccatatca gacatttact attactattc 360 aaaccatatg cagttatcca tctcatactt ttggttgtga 400 <210> 12 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 12 catcaattat acttttgatg gtgtttttgg ttttcctatt acatgctcgt ctatggaaga 60 actcagagtt gcgatctcca tctttgagcc aagtaataat gcacgatcgt tgtctccagt 120 atttttcttg cgccgccaag atcttactga gctgaatgtg aagcagtttt ttctcttcat 180 actggtgtgg agaatatggy tgccgcatca aatcgttcag cttttcttca ataattcttg 240 cttccttcct ctgtttgttg aaatcagttt tatgccattc cataagctta cttcctgcag 300 ttcgaatctt gtaagcaatt tgtccaagca tgtttctagt agtaggttgc gcccacgctt 360 gtttgatgat agattctgct tctgcttctg ctcgaccaga 400 <210> 13 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 13 aagctgaaga gtcccacatg catgcaagtg aatcaaacag tataaagagc atcatgaata 60 attttgcatt ccatcttctg aaaccaagac ttttgatgtt gtttgacaag caatgatttt 120 gataagcata tttatgggct gtacccccta gcatctataa accttttgga tcttcttaac 180 caaaaagata agactttcar aacaaacacc tttcttcatc aaactggata ttgaaagagg 240 cttaagctat tcagatgatt gttaacttgc tattgtacta taaacttttc tttaattatt 300 gccataaaag ggctcgagtg ctattgataa ttaatgtttt gtaacggcca aagaacaaca 360 aagtaactag acatccccat ctagaaaact gcacaaaaat 400 <210> 14 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 14 cagatatgaa tgacacattg ctctgcactt tcatcagttt catgtagata atgagtccaa 60 gatcaatcct gctatagtgc tattatcttc taagttgttg tagggccgaa ttgctcatct 120 atgctgctat atatttacca tgtatgcata aacaactaaa tgaattgcac tatgtgctgg 180 gattattcag cttatgattk gacttcattg atgaaggttt ccttttagtg ttaggatctt 240 gcttagtttc tttcagctac tacatacagt agaatattat gggattaatt ccttcataaa 300 tttttctaga ccgttaatct aacctttgta tttgcagagc cattggttgt tggaactaat 360 tcttagcaaa tcgtctcaac aaggctagaa ctcaagatcc 400 <210> 15 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 15 atgaagtagc aagttgcagt atcaatgagt ggagagatat gataaaaaga agcccaacaa 60 aaatgcaata ttgcaagatc atatgcttta cttgtcaaaa actacaaaat ccaaatgttg 120 gtattctaaa gtatccaacc acattgagca ctcaactagg cataagaatc aggtgtcgaa 180 cagtaaggct aactaaatam atgacggagt gtatgcaatg cgtacataca tgtgctgtgc 240 attaaaatac acacaactac tgaccacatt gtagctaatt tcatgataga actacttaca 300 agtacctatc tttctccgat tcacatacat aaagcttatg ttcacccaat tctcacaaaa 360 caagcatacg aaacacgaga gggcttaggg ttacatacaa 400 <210> 16 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 16 aataagaaac tgtttattgt tcatcgatac acactgccaa gaatttcccc accatacacc 60 aattgaggcc atgcctgggc caaggactct tccttgatct actagaaatt tcggatttgg 120 ctttcatttg aatcaatatc acctataggc atcgactgtg ttcatattcc tctctggaaa 180 catgccaacc atttgatgak gagaaggcac tgaagtgttg ctcaaacttg acatccctgt 240 tgtggcaggt atattaacat tctcaggtgg aggtaaaaca gtagacaaaa acctactatt 300 aaaaccaaac ttaccagtga atcatcaaat tatgaaaagg aggcacaggt catatcccac 360 taacaccact gttaaccaac gccaacccgt ttggggaatt 400 <210> 17 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 17 catttccatt gtattccgta gcctgccatc atattctatg gattgcaatt ctccatgtga 60 ttgattgtca ttgatggtgg ttttactggt aaactttgat tactatgttt tgttatggag 120 aagactgcca agatttagaa tttgcattct tcattcctgt gtttactgtt gtatatattt 180 ggaaccctga taaccccgck gtcggttcca tttattttaa taattgtgga gttacttgta 240 atttctgtct tggtcttacg gaaacagaga caaaaaaaac ttgtatttac aggtgttttt 300 ctgtgttcag agtatttgaa gatgcttaag caggcagaaa gaagctctgt ctttgtattg 360 gttactcatt ttgagggcac ctacttcggt gcattatatg 400 <210> 18 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 18 ttcttgaatg atagatattt aaaatcatcg aagggtaaat cattgttcat gtacttggac 60 ctagtaatca aagaagttgt gttaacacca acagcaaaaa gatacaaatg aaaaatacat 120 gcatgactct attcaacagt ctagacaaac tacttattaa tcaccaccaa aaaagaaaag 180 tttagcgctc atgaaagagr gagcaaggta tgaagattat tgcctgtgtt gtttctagtc 240 tgctaatctt tttcaaaaac tgatgaaaca cttactgaga taagttcttc ccatgtgtag 300 ttgctttgac tacacgagaa ctcgcaccca cagatttaga acccagttgt tgattgatct 360 gctttcaaca caagttaaaa agaaaaacta cgtaagatga 400 <210> 19 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 19 ccattctatc cgtacaggat gcccttttgt aatataattg cgttcatgat tcagacagtt 60 tactttggag cttaagcatt tgcaggattc atttcatctg taaaaatcat cagtcttctt 120 atttgggaat caaatttcga tctgtgttga aaatgtagat atttgcccct ttcttagaat 180 ctccttttgt atttgattcr catgccaaaa atcccatgtt cttcgttgag aatatgcatc 240 tgggtacatg accggacgca atgcaacctg aatcgcttga gccatttgcc ggattggttt 300 actgattagt cctatttgtg gatcggtttt ctacctgtct gttttgctca caaacctttt 360 ttgttgtaat tgatcggtgt tacccaaaca tgtattgttc 400 <210> 20 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 20 aaataagaaa ggcgaagtat taaaacaaaa tatttacatg cacgctcctg ttcctgagct 60 taatacccaa ctacccaaca aagaacacca aactgtctca acacaaatga aaaagagaca 120 caaacctgta aagacaaggt actcctgaac cccaagaaag atacacccaa atcagcatag 180 tgctttgtct gcacagctcy tttaaaaact tcaatgaaaa caacataagt acataacaca 240 acctgcaata caatccaatt cagagaccat gaactgtagc attttcacat accgcaataa 300 tgaaagctag cttgactata atttacaaaa gctcgttatc agtactaccc tgaacagaaa 360 cttagataag ttatttgtct ttatttttct ctcagtttcc 400 <210> 21 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 21 gaactcacga tcgacctatt acaggataca agttgtttat ccgttaaaat aaaatctaaa 60 aaacaaagtt ctgaaattga ctaccaccgt ttaaattcct cattgtgttt tttaaatctt 120 ttcacacgca agctgagcta actggtaata gtattgtatt gtagctaaat aatctttaat 180 taatgctaat ggtgcaggtr ggactattct gtctcttatt tggttggata aaagaccgac 240 gtgatgatca aaaacaaagg cttggagact attccaaagt catgaaaacc atagacaaga 300 tatgtatctg tctccctttt ctttttcttt ttattatgaa tgaaaagaag cgaaaattat 360 aattacaaca tctgagttgc aaatagcaac agcagctgtt 400 <210> 22 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 22 tatgaacact gaacagttga acacagtttt ggcacataat atggggtcaa ataggattga 60 agatgctgtt gatggatttt tcattcaaaa ggagtggaat caaggtagat gagacctcta 120 tttctgttta ttgatgtgaa caaggtctta aaagaatgtg aattacaaaa ggtacccaat 180 tcaaggacct gcagttgcar tatcattttc agtagcttgc ttattattct tttcattgtt 240 ttttcaattg ttaggctttt cttgcttaag atacatctct atcgtgtaaa tgcgtaatgg 300 tgaagcaggc atttctttct ttttctgagt cttttctcca tttttgttta gtggtgtttg 360 caagctttgt tcatgttttg cctctatggc tctatctatg 400 <210> 23 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 23 gatcatatcg actggggatc tggaccgacg gctcctgcgt aactggaaac ccaatgggat 60 caaagaaact tgctcttttt ttcttttttg taataataga aaattctatt aaaagaaact 120 tgctctctac ttgccaagaa atgcccaata aaaatctcaa aattaagtag ccatgagttc 180 tcaatcactg tcagctgtay gtgtcgtcta gctactttca aaagatatat gaaagaaaaa 240 gaagaacaag gtgatgatga gatttgaatt acccgggttt cggggtttta cgtgtttaat 300 ttattgaaca cgtagcaaaa atagccgagt aaagataatg atcaggtagg caaagcagaa 360 atatggtcac agatcgagct gaccatgcat tccgtgctag 400 <210> 24 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 24 gctcgcaaga gatggatcgg tactcgtacc agagtatata tcaggctgtc cagaaattat 60 ttgcataata attattcaat tatattatcg gaatcactat gcagtgagct aaccacaccc 120 caattacgtc cctgttgacc tgtgtttggc ttgtccaaac atataccgta cgtacagctc 180 cgaagaaata tattgagatk gtgtaaattg ttgtttatcc acaaaaattt gaccacaact 240 acaaatgact gaaatgagta gaacaagatt accaagaacc tcttggtatt ggccatgggt 300 agtctttgca tggtaactag atgtatcgga taccctcatt tacaactaat ttgacaaata 360 ttacaattta cttgaaccaa attatcattt tcaacacaaa 400 <210> 25 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 25 ctctaggcat gcagctggta cgtattatgg tgggagctgc agggctgtgg aatgttgtgg 60 tagctagttt agtcgaggcc accatgggag cacaaattga aaggctttaa acggatcagt 120 aactgatgag aacttttgag aggtttcagt tgccgtcaat gtacgaatat tgcacatgag 180 gatgtgcaac gtctcagatr gttgttcctt acgccataga tacaatacat cttcatcatt 240 tgactcaaaa gctagaagtc gacatctgga aacccgaaag caaggggtgc agccgtgcag 300 gtcgaccaac tctttggaac atcattcgtc atcttctttc aactcccaaa tatagagact 360 gagtctgctt tcaggtcctg aatacaactg gcatagcctc 400 <210> 26 <211> 395 <212> DNA <213> Fragaria ananassa Duch. <400> 26 aaaaaaaaaa aaaaaacttt gtatgaatag ttcctaaaaa ctaccagcca tatgtactga 60 gcgcaaacaa aaaaacaaag cagactattg acatttctac aaacatttga ataggaaatc 120 acaagcaatg ggatgaacaa tacaacaata caaatatagg gacaacagca gaaggagatc 180 attcagaaac atgamagtga ttgctcctct tgggtccaag ctaataagca atgggagatc 240 atttataagc taaaatttag gatttctaat caagggtatg aaaattgttg ttcaatagat 300 caacctcaat agtctatttg tctcctcagc attatatcaa cagccattaa ttactctatc 360 tctttaaagg tccatcgatg tcaatcatca tatac 395 <210> 27 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 27 tgcaggatac tctaacgcag catggagagc agacccttca caagccagtg gtggttatgg 60 tagttctcag tccaggcaga cttaacagca gaggtccaac agagttagaa tttctttcac 120 aggaatcctt acacatacga tctttcccca acctaaactt atgcatgaca agagttgaca 180 atgaatcttt gggctggtty gatcgctcga ttcttgatgg tagttggcag cagtatttct 240 gctagaaatc acgactatta cattctagca gtattatttt ttagtaaaat cactttaata 300 ttagcttgct tagactgtgt gtgtgcttgc taattttgga gctaccctca tcctcattca 360 actttctctc ttccccttgt tgtttacact agatatttct 400 <210> 28 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 28 cagagtgatg tattggagat ggtgaggact aaggagggtt ggttgcgcaa cgatctgggt 60 tttggaagta gatggagggt cgtgggattt agaagaggta gagagggaga tgagggtagt 120 ttagactttt ttttgtcaaa tttagtgtca aagtcggctt gtgatgttat gttttgagtc 180 aatttcgaaa tttggacgar actgatagaa aataaaagta caaggaccat gctgattaaa 240 aaaaagttta tggaccaaat tgattttaag cctaaaattt aagagggtaa actgaattta 300 atctatatca aaatcacctc acgcaatata atcaatcaaa catatgcatc caaaaaaatt 360 attcatgttc cacatgtctc aacataatca caactcagtt 400 <210> 29 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 29 caagccctat gcatgcacat tgacactagt aattaatcgg tggaaaaatt atcacatggt 60 cctgaaccat ataatatatt acatgcggtt tttttttcaa acaaaatttt ttattaaata 120 aaaaagatag tacacatggg gaactgaaac taaaacccct tacaaacaaa caatagaaca 180 aaagacaatc aaaacataar aacaatcaga gctgattgta ttatagatca agacacacca 240 cgggtgtccg attacgggca tggtaaaggt aatatagtac aggttgtttg tttctcatcg 300 tctcttaaac ttaatttctt ttctaatttc cacttcttca tggtcctcac atgattcagg 360 tgatatgatt ggctatgacc atcacatgac agtgtcacgt 400 <210> 30 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 30 atctctgtag gtttggtaag ttgagttctt tttttatctt ttttgcttat tttgtacttt 60 aaggaaacca gaatcacaac agaaccagaa agaatgtgtt tctttcctct ttgaagcgat 120 gaaacggcgg cggatatgtt aatggtaacg tcagattagc ccacgagaat tttaatttgt 180 tattggatat tttatcttam caatattgga tttgggtact caaacttgcc accactccca 240 ccagctctac aagaatgaca ggaagtattg ttgctactct agcttcaatt aacatggtag 300 attgtaacac ccttacatct atgatttgtg tctcctatgt acgaatgaga ttatttgttc 360 gaaaagatca tttaacctaa catacatcga cggaggtagt 400 <210> 31 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 31 taaaaatcac tttctcctct aaaaaaattt gttgacacaa aataaaatat atttttatga 60 tataaatgct agtgaaaact catttggtgt ggtaaaatgt tgtctaattt aaaacactca 120 tgtttaaagt ttgagtcaca actcccatcg gtttgtggtc aacatgtttg aaagaccaac 180 atttgggttt gtgcgcctgr ggcgaaagat tagtttgact ttcctcgaat cttctagtag 240 acctcggaac aaatgttgac taggtggatg tgttactaac ttactacgta actgagataa 300 cttattacat cactcccaag aaaacaaaat gatagtggta tgatgtttca caaaatatga 360 tcttaatttc atgacatatt agatatatat tgagtattaa 400 <210> 32 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 32 ctataaaagg gaagcctccg atgatggaga acatcgagac aacaatgaca gagaagagct 60 actccggcgt agtcgtcagc gaacccgccc cgagttccgt ggagcctttg aaccgcaggt 120 ggatgtccga ttcaaaccga ggtacgtatc ttcttctccc tccggttccg gttctcgtac 180 ctcttgccct atcaattctm acctatttcg ttttcggagc agaaaagttg agagaagctt 240 ggccgatcgt caaatcgtta ttggaagaag tcgggatttc atgctcgatg gatacggtac 300 gttcaagtct gtgaattttt ggaaagctag ttatgaaatt gagattgaaa tttgaatggc 360 acactcaggt tgattccgca gtcacagtgg agtcactctg 400 <210> 33 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 33 tgaaagcctt ccgtcctcca ggtgaagcgt atagaatgaa tctaatgttt tcttctgata 60 cacagaaaaa cactcttact gctgtgggtc catctccaag actaggctgg gtcaggaggg 120 ttttgaaaat ctgtataact gaaaagctga gtccctcgca tgttataagt gtgttcatat 180 gcaaccatga tcagagaaty tcatttgttc ggaatgttga tgtccttaat gtcaaaaaga 240 ctggccattt gatgatcaaa gatggtgcag ctgaagtgat gttgtccttt ctcgaaggac 300 gaggtacatt catctctgtt cttgttggtt gctcagtatg cttggaaaca agcatttgga 360 ttgaaatgaa gaaaacatga taactaagtt catacgcgca 400 <210> 34 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 34 gcccccatct cttgagcagg tgtgttatag gatttgaaga attagtttta cataaatagt 60 aaatatactt ggtgttataa cccaaaatat aaattaattt tctttcactt cttcagctga 120 agctgataga ctgtgtcaat ggtgttcaat ctcttcctcc tcctggggag caagaaatgg 180 tgcgaccgca agtaggtccr attgaaacac ggggagttct tggaggagga cctcacatat 240 acccgctctc cagttgggga ccgcttgatg ctctgctgtc tcaattggat tactccttgt 300 ggtgttgcgt gaattttgag attactgggg tgtggccatt caatgatcga ttctagctaa 360 aaggtttggt tccaatttag tgcatttcta ctctgtctat 400 <210> 35 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 35 gatgaagaac gaaacaagcc ccaagaactt cctgtccaag ccttaacact cactcatgta 60 tatattgtta taacaaatga acacttgaaa tgctagacct tagatagcct gcttttatag 120 tttggatttc aagatctggt gaggggaaat ttagtctaga aaatctgctg ttatcgactc 180 ttgataaaaa tggcaatcar ttaagatttg ctcaatcaat cttctgagtt ctcttacata 240 tgccgcacca ttaacatttt ttttttcttt ttggttcttt attcatgaaa cttaattaaa 300 ggacatttgt ttttcctttt gcaaaaatgg tttatacaag atcccaataa agcaaagtta 360 tcagagaatg tgatggcttg tttcataatt ttagattaac 400 <210> 36 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 36 gtcagaggtt tggatcccgg tcttcgattt gctatggtat ggggaatcat caggttcttg 60 gcgttgtggg gaatgatcta ggaggttttg tgagagcaga tctgggtccg atcaagagga 120 aggagaagat ccagcgtgtt cgggttgtgt tggatgtgcg aaaccagatt ccagcggaaa 180 atatctttaa ctcctcgtcr gagatttctg tcaagctgag cctcaaggtt gaaagagccc 240 acaggatttg cagggagtgt ggcttctttg gccatggagg atgtacatgt gataggggtt 300 tgatttgtgg gttgctagtg gtggatgcag tggtgaccga cggagagctc gagattgttg 360 aggagccaga gatgcaggcg gaggaggatg aatcagaggt 400 <210> 37 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 37 tcagttggat gttttctcca ataatttcta cacttctttg ctcgatcaac ctcaatggga 60 cctagcttga caaagaacct acggacccca ggggtctgcg tggcttggga tcccaacacc 120 tgtgcaagcg gccataatca taaacgttct cctagctaac agattggtgc ccatggacca 180 ccctcaagct ctctgggatm gaaacgggta agcaattcat ggttgagtcg agatgactca 240 gatcacagac gactgaataa ttcacaagcg agtttcagat gtaccccaaa tgagttgctt 300 gatttgaggt cactcggcat atttattgcc aaaacgtatt ctctgcataa atgtgcgcac 360 ctgcaaaaga tgttgtgaac ctcacaactt tcggataaga 400 <210> 38 <211> 257 <212> DNA <213> Fragaria ananassa Duch. <400> 38 aaaaaaaatt aggaatcata cattagtttc tccttttata tataattaca tcatacrgtc 60 caaataccat cattttattc gtttcaacaa tcaccagatt atctgctata tattttatgc 120 atccaagtaa ataggatttg cagttcccta agcataatca gacactaaac ataaccaaaa 180 gaaaattagg tttcacctca gcatcgagac cgaaggaatt cctcccaatc acatccttta 240 aatgtgcttt ttctaga 257 <210> 39 <211> 295 <212> DNA <213> Fragaria ananassa Duch. <400> 39 aagtggagac actggttttt ggaggcttag gatttcctct cgctgtcttt aaatttttcc 60 gtattggcta ggcattgaaa aagattgttt tcatgttttc ttagaagaat cacatgatgt 120 gataagtcaa gttgtttact atagaaccct catggtaaga ctttgagtct cgcgatccta 180 actacaaaga gaaagaagay agtgcacatg cactaactca aatctagttc ttgcctcaac 240 tcccacaagt ttgtgactag caggattgag agacatccga gtttgtgcat ctgtg 295 <210> 40 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 40 actagaaaat accttagaaa tataaacaag attacacatg acagtagaaa ttaagcagca 60 acatgtaaat agcttacaag acctgtcaaa agtttgcgca gcattctcaa agatcatcaa 120 tgccctacga agtataagaa gaagaaggtt gggtaatgtc tcgtggagaa acttagcaaa 180 ggctagtggt caaaggaccr attttcgaac tgggcgttcg accttcttgt agggatctac 240 gaacagaatt ttcaccgtag acccgttgtc gaatagaatg cgactgacgt tgaagccatc 300 gataaaggcc gtaaccagaa atggttcgta gacgactcca catccttcct cctcgcagaa 360 tgtaataggt gcgcacccct tgcgctgggc tttaacaggc 400 <210> 41 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 41 tctctgttgc cgccaccaca aggagagcct taggcgaagt agcacgacag tttgattcct 60 tggtgcttct tgcaaatttt cagacacaga caacatgtta agtcatgcgg ctttcaagat 120 tcttaaccaa aaaatgaagc tagcggggtt ggacgtagtg gacggtgtac ccaagagagt 180 agcaagtaaa gttcaatcgr acatgcctgt tcacacccat tagaaaaccg cttccgggga 240 acctcttgca gatcacaatc cgcttgtggg ttgacgaggt cgctgaccaa gcttcatatg 300 aagatgtcgt tgtcaccaaa cagaaaggta aggatcatat tgtgacaccc ggaaatttga 360 caagcttaat tagaggtcat ttggtaaata gtttattgac 400 <210> 42 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 42 caaaacatat tctcaagaaa actatatatg gactcgggga gtacgactgc ctccaaaccc 60 aatgagtgca aaccaaagag cagttctccg ccgcatgcag tctcaaaaat catagggctt 120 ttagcatttt catcttcaca tgttagagag ctagaacaag agaaggagca cattaatttg 180 ccaagtcaac ttagttaggk tataactcat aatatcttaa ctttgccaaa agtgaaacct 240 caataccact aagtactaaa tctattaaac aaactaatga tgaatgagta tttgtatcat 300 caaacaaaga ccaacgacct tgcatctgcc tccttttgac aataatagat caccagctgg 360 tgaatctttc tacctaaaaa cacaagtgat cgaccatgca 400 <210> 43 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 43 acataccaaa gcctaagctc taaccttaat caatcttttc ccctttttca atttccatat 60 tactgtttgt tttagtgatt cataattcta attaagataa tataatccct tgttattgtt 120 aaatcctgct tttgcaagat catttgtacc tttatcaaag cagaggcaga catgataagt 180 gtttaaatat caatttgagr aaatttccca gccatgaaac accagatgaa ttgtcttctt 240 aaggtaactt ccctggaata ctacagaatt attgtttcta ccggtgtttc atcatggttg 300 cagtgactta ctattagcgc aggtatgatc aagtagaaga aataaaggca aaaacaagct 360 actcggttcc aaatgatggt attggtacct atagaaaaca 400 <210> 44 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 44 ttgtttgatg tctctccttc attgaaatcg gaagaggagt tgttctttgg gaagaccaaa 60 gggtttcaca gaacctgtgg attatccatg cacgatgcta ctggatgtga tcaatatttt 120 ctaatagaga tggatgaact catgaaggag gcttttggtg gacacactgc tttgtatcct 180 tctagttcca gtgaagctak aactgatgtc aagttctact atactttgga gacggactct 240 attgatgaac ctattgatat ggttttggag aaggtgcatg ttctattgat gaccaacccg 300 aagaagggta ctttgcatga gatgtccccg acgagggagg tcgacaatat tttcacggct 360 aatgctacct tggacgtcat tattaggttg atactaatcc 400 <210> 45 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 45 ttcttttgtt ggcttaacca cttctttaat gtcttcctaa ttgagaacta gctaggagat 60 catcaactcg atcacgaggg ttggcacatg acttggaatc atgccccaaa aacgcgttat 120 attaacaaga gaatcaacaa acccaaaatg actactcaat attcaccaat taccatctcg 180 gataagaaaa ctcgaagcam aatgaagact aggacatgga aatgacactc ttggagtctt 240 ggggacccaa ttttatgctg agacaattct tctatatgat ggtcgagaat aaaggttggc 300 ttttaacaaa gatgggaaag taatggctaa catgactgtt tagctggtga cttaaggatg 360 gcaaagtgtc actgttaagg gcagtgatcg aaagcagata 400 <210> 46 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 46 taacaaacac caacttgtaa aaattactac tctattaatg actccattta ttaggggatc 60 accaagcttt gatgacttgg gaactctata atgacgtcaa ctgcatcaag ctagggaacg 120 gatactccat aatataagtg gaacatacaa gctgcaagca gccaacttgt tctagtctgc 180 aaaagcaggc aagttatacy actaacgact gttgtatgca atattgtata ctactaacca 240 ctaccttcct aaaagaaaaa tccaacgact atcaatagct accttcaatc agtacaaaat 300 ctctaccgca actctgcctt cgaaggacca ctacgacacg ttcaatgaat gagaatctga 360 acacccaccc ctagtcgccg ccttaaaatc aggtctaatc 400 <210> 47 <211> 246 <212> DNA <213> Fragaria ananassa Duch. <400> 47 atagttacga tatatgttaa tgggtagata tatcttgggt attgctgtgc aagttctgca 60 aggatggatc ttgcggagga tttgaacaaa gaaaagcaaa caaattaatg ctgcccagtc 120 tcacctatat atgatctgtt gcagaggatc tgaataacga agtttggaag gagcaggtgg 180 aacccatctt tgaaaagcay ggcctaacct gtagaatgga tgaggtatcc ttctttattt 240 ttctta 246 <210> 48 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 48 tagccaactg aattgatgag gatatcatta ctaagttaac atgtagaagt atatattcaa 60 atcgttgtct aggctgcagc acagccattc aagtcgatcc gcgcctctac gtggcatgtg 120 tagaaatcac gtttgggcta tgttgctgag ttcttgggtc tatttggatt ttggatagtc 180 tgttgtttct tttgtagtty tttgtttgca atgtaattta gtttttagat ttgtttttta 240 gatctcaaat atgtccttac gtttacgaga ttgttggata tatgtcatgg atgctgatga 300 atgtttgaat ttgggtagct gggatgacca agaacccatt tttttgccga ctgttcgccg 360 agcatactat taatctgtgt taagcgtccc gagagatctt 400 <210> 49 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 49 caaaagtcaa ccaagcttgg aggagaaaga gagtgatgac tcagtaatta ttggcattcg 60 gtatgggaaa actccagatt gaaacggatt ctattccgaa actgttccta aaatttcatg 120 gctggaaaca tcgtctaata cataaacgac attaataaag attcaatttt cctgcaaata 180 ccaaaaaaaa taccattgay cgagtgcaac caacctcatc cggaattcca gttttggatt 240 tctattaaat gtgaggcatt atatatacta taaagagacc cagcctctat attagatgaa 300 aggaaatgaa gaaagtagtg ctcctaaaac gctttctgca ttagaatcga attacttctg 360 tctctgcttt tcttgctcct aactgtttct gcagctggac 400 <210> 50 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 50 tcactgttgg catgacatga gagccatgct tggtgttaga tcttgacaca ttgagaagtg 60 cgtaaagggt ctaatacatg tgtcgcatat ctaacctttt tccttgaatc acttttagac 120 catgcctaag agcatgattt gtgtcaaagc tcgacatttg acatggttga tgagggtagg 180 ccgaccatgt ttacatgcar cttttgaatg tccacaagag tgtttgaggt tcgaggctct 240 taagctttgg aggtcgaact cttagtctta gattttgagg ctctagactc gtcttgtgtg 300 tttagatcat gacatgtttt tgtcttgcca tgtcagatgg tgaggtaacc acttggtgac 360 actccaagac ttatggtgga ggtccaaagt tgacaccttg 400 <210> 51 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 51 atttaatgga ttactgtcat cctttaaaaa aaaattacat ctaaaataaa cgaatcatgt 60 ttggttacaa gtttaaatgt atagttaccg agtttctcgg agcagggaga aaattttgga 120 aacctagcct ctaggttttc gatttggcag ctgtgctctc cctcttggtg gacgttgatc 180 gtgggttcta gaggcggcar cgcgaattca ttccaacgac aatcatgtgt ggtggcgagc 240 gaaggatcag tgttttgcga cggcgaccgt gcctttccaa gtgtcgggtt tgatcaagac 300 cgtgcgatca gttatgattg gctggcaacg gcttgatctc tatcagaggt cgggcgatgt 360 agcaggctgt tagaacttca ttggtgtctc agacttaagg 400 <210> 52 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 52 tctttgtccc atctgggctt aattgtcaaa gtttagtttc caaagctttt tgtgcaaatg 60 aactaatgaa agaaaacact tattgttgtt ctggccatgg atgtcaatgt agtttggaaa 120 aatatgtcta agttcgaaaa aaaaaaaaac agaaattgta gtgcgtgact gcttcccttc 180 acattctgga atataattgr agattaacaa agactaaaga agattgattt cctcgtccat 240 gtgcagcata tgctttaccc cgtgggttct atgtattttt tttttaaaga aaattatatt 300 atgtctcatg ctttataaaa tatgtgtcaa tcattgattg aactcacgac aagaaaaaca 360 cactaggcgg gcaatagttc caagcaaccc ataacaaaag 400 <210> 53 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 53 agtaaaatca agaataaaat gcagtagtct tccatcactt gccttgacaa ggattaagga 60 aatcaagtcc tccttgccct aacccctaaa cagactagaa aactagattg ggtgataaaa 120 cccctaacta agtcatcacc gaaaggcttg aaagggataa agtccaaaaa tagcacctag 180 cctaactaga gcccatgcar ccttaaagga tagacttagc ccagacccaa caaggtgtag 240 agactagatt cgacaaccaa tgctgaagtt tgacgttgcc tgttgcaact ctgccatctc 300 gtcagccata gtgcatcacc acaagcctca cataccgcga cactgcaccg ccgccttccg 360 accatgcctc cacgatcagt agcaacttat agatcaaaga 400 <210> 54 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 54 tagctcttcc attaacttga ttgtgtctgg taatgaatat tacgatttac gaatccagtg 60 atctttattg gctttctggg tacaactagg aggaaggggc ctttggatag gaatccaagt 120 tggtgctttt gtgcaaacca ttatgcttgc ttttgttacg acttgtacaa attgggataa 180 acaggtctgc ttctcaaccr ctcatttttt gatgatgctg atcttggtgt aatatatatg 240 aggttgatcg ataaggttgg actgtgaaac tgaatattag ttttttcttt ctttcttctt 300 cttcttcttt gtttcttttt atttgatgtc aggctagtca tgtcataagg tcataagagc 360 ccattagact gtgaatggca tatttaatca tcaatatagc 400 <210> 55 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 55 gagctctgtt ttgcaagatt aactgtgatc gttgttactg attttcacca acaaacaaca 60 agcactttgc ttagttctag gatgaaaaat atttatattt atcataatat gaacgtactg 120 ggagacaaga agagttatga agttatgatt atactcgtga aattagcatc cgtcatgcac 180 gaataatctg agtatcatcr gcaacttgat gcaaaatacg tatatctttt tcattaccta 240 acaaattggt tgtcagtgtg catagagaaa ccatgcactc tttttcattg ctaatctgtt 300 atattatcta tatagaaata aatgcatgta tttatcgtcc ttcttctaat taaaggggaa 360 aaaaatctat aaaaggcatc aaggcaataa tgtgtgctgt 400 <210> 56 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 56 ttttttggtt tggaagaata tcaatggata tgtcaaactt cagctctatt tttccattct 60 ccatatacgg agtaatacca atgtcgagat ctcgtcacat ctacaattgg ctctacattc 120 agaatagtct caaacctcaa ttgaggataa tgaggaattg ttaatgccca caaatgtgat 180 tgtatttccc aaatccattr cctccgactt gttcgaacac cttagaacca tcgcttagac 240 catttgtaga tatttgtata tataatgttt gtctctaact acctttatgc ttaattgaca 300 caccaattat aagaaaacta tgtaagaatg tgtggtctat gtttggtttt ccctagaaat 360 attctttgct atctatgcgt atggctgctg gtatttgttt 400 <210> 57 <211> 265 <212> DNA <213> Fragaria ananassa Duch. <400> 57 gccgtttagt gggctgatca aatgaaatga aaaggctaag atttcatttg aattactggt 60 tcatttccac cttccgcaga taatagttat cttttgcaac gcccggatat tatttttact 120 gttactgtca tttggacagt caacgattat catttttata tttatttaca tttttgaggt 180 ttaagccgag atgactgttm tttaagccaa agggacaaca agacctagat gagaacgccg 240 cctctgttag tactattatc ctttc 265 <210> 58 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 58 tgtgaaacca ttcaggactt cgaactgtag taacaacctt ctgcaccatt cacatagctt 60 aatgtagtta tcattttcca tttccttgga gaattataga gttgaccggt ccagtattaa 120 cttcattatg gtttgatgtt cttagccctc tgtcagtcat gaagttgaaa tatgtgagtt 180 ctgagtcttt cattctaaay aacattattc ttgacttgac accaacaaat atgtcatgca 240 gctctacaag gcccaaagga atgtaattct ctgctctgca gcatgtcttc tttactggta 300 tgttggttag ctgtcaaatt cccttacatt ttctctgaaa tagaactttt gtgcccctaa 360 ttgatttatt gtggtttaaa atgttgtaca aatctttaca 400 <210> 59 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 59 gtactcggtt tgcgatttga tttttcacta tgttgattgc tacgcacagt agattgttat 60 tgctagtttc gcttccgcta agttgggact ggtctcagtc tttatgtgta aaagaatcaa 120 tttgctggcg catgagttgg gactcgtcac agtgtttatg tgaagaagaa tcgacatgct 180 gctgtattag ttttcgtgck gattctttaa ttatgtcatg aggagtggtt taagagtctg 240 taattttttt gtttttgttt tggttcaata tgagttgtga gtcggatgtt aaatggagtc 300 tcataagtag gctgaagagt gatttcataa gaaaattgat tatctattac tttgttttaa 360 tttagtattt gtcgcctttt atgaatttgg acaaataagc 400 <210> 60 <211> 347 <212> DNA <213> Fragaria ananassa Duch. <400> 60 gtggatcatg gtagtacaga acatcatgac gtttaggatt aggttggatc tcaaaggtgc 60 actccaaagt atcctaaaaa gctatgtagc ttggtcctga actttttatt gcatccaact 120 ttggatggat tattgttgct aaatttygtt gaaaaagcta gctagtgtcc aagtttttgt 180 cccgatacat tttctttttt caaattacga aataagtgtg agtgaaattt ggatcataaa 240 aagaaggaat ttgcgtcaaa aaatcaaagt cgtcaaagta aaagaacgag tttgatgcaa 300 ctttcacata ataaaatctc atctttaaca tcattttgag tctacac 347 <210> 61 <211> 307 <212> DNA <213> Fragaria ananassa Duch. <400> 61 aagaaaatta tgtgattgga ttgggccact cgtcagtttt gggctttgga tactttgggc 60 ctgaagttta agattacatc acttacatgt ggtcgagttg ataagaygta agacgaaaga 120 tgtggaactc ttataagtta taaccaggtt tgaatcccgc caatactgat tgaagttaaa 180 gaccgataca ttctccacgg ccaaggtaga taaagcattc tgacatgatc cccggtcatg 240 gattagttgc tatgcctaaa atgcttttga ataaaccgtg tgatttcaat aaaaaaaaaa 300 attctaa 307 <210> 62 <211> 249 <212> DNA <213> Fragaria ananassa Duch. <400> 62 ccagtattcc taggataaca atctgtagtg gggaaatata tatgtagtrg aaactaatcc 60 catatcgaga aggagttaaa aattatgcag tttataagtt aacacgtact cgataatatt 120 tgctgaggcc taaggtgaaa acctcacact tgaaaatccc aaagggaatg ttcataaaca 180 atcaagtgcc aaagtgagga caatatcggt ggtgatcaac ctcctccctc atcaccgagc 240 gaggccttt 249 <210> 63 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 63 ctcataagca acataagcag ctagcaatct atctgacaaa tagtaaaaaa aacgttcgta 60 ttataaatct ccaaatctgt tttgatattg actggagcag ccaaggaaaa agtacactac 120 caagcgcatt catgatatag agactggcat agattggtta atactagcaa actcaaaaat 180 gaatttgttg attggaaagr cacgcagcca cgtcgtagag acaaagcctc ctctcattgc 240 tcagtttctt cttgttcttc ttaagacaaa gcctcttctt tcttcttgct cagtttctgc 300 aaccactcat tccccacttt aagaagattt gcaggttcct cattgtttat aatacagcgt 360 ttcgcaaaga tgaccaccat atcaagttcc aggtcttgct 400 <210> 64 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 64 accttgaaat tttgataaga ttgtatcgaa attcgaaaat ctcttcatga ttcttctttt 60 tatcatcaac ttattcttaa tttcatgtct ttctctggtt cttagagtat tagattacct 120 tcttatttat atttgatcgc atattcgaag ctatgcgtgg agaatgatca atgaggataa 180 gccatcaagg aagaaagtty ttaaaaaacg tgccgctcaa aaggataagc aaccaaggca 240 agaatttttg gaacaacgta ctactcgaaa aaactattcc aaagcagaat atgttggaaa 300 aaagactgaa catcgagcgt aataactttc aaataaaaga agataatata agtaaggact 360 ataactttca aacataagaa aataatgcaa gaagttttgg 400 <210> 65 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 65 taggcacata tgcttaactg cgtacttgga caacacttaa gctaaccata acagccaaac 60 tcacccaaat tggaaacatc aaaggcaaga cagcagtacc agctacaagt agtagcaagc 120 cgctcgtcaa acagagttag gacacgccca tacgcgtggc ttcaataccg tttgacagaa 180 caacaatatt gagggcaccr gtcccacatc aaagaagagg ataagtcacc tacaccttta 240 gcttatagat agcaactctc tctctctctc tctctctctc tctctctctc tctcttcaat 300 tactatgcaa ttacttttta cctactgtta ttatgttaac attaatattg aattaggcat 360 cgaagcatag aagatcgctc atccgcagtc tcttcctcta 400 <210> 66 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 66 taacctcatt acaagtcaac gaaaatctca ctaaatagcg agcatataaa caaaaaaatt 60 tcggtttcct acaacaaatt catattttaa attctcatta accagtcacg caatgttgaa 120 taggatctcc agcaacacta aaaccactgg caagctagat agaatttgtt caacatacca 180 aaattcaaat tatggctaak actagcattt ccattaaatt tgaaacggaa atattttagc 240 taatttaatt atttttgttt cttaaaaatg ggcaggcgcc tatgtaacgt aaaacatttt 300 cacgattgca tgaacaagtc aattgtggct aacatttttt ctttccgtta acagctaatg 360 tttcgttcta cataagctgc caaaacaagg tgctaatcca 400 <210> 67 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 67 atatgtttat tgttgggggg ccatttgaga agcaacatgg ggtcgagtac cctgcagaga 60 ggcctttgcc tctgaagttt ggtgacgcat gtaagtgtcg tgcgctctac tttatatttt 120 atatttatca cttaattcca atattaacta ttattcttgt tgtgtagctg agaattgaaa 180 tttcgctcct caagataaar cacttcaaat atgaagaaag tcttcacgat tgtccttgaa 240 catggccccc tttcctttct cagagcctgt tcaattgaag ctcttatctc gcgtggattt 300 ttgtaaagcc cagttatgca atgtttacct tttttgtttg agcttgactt tatgcacctt 360 tgcttattgt ctggtgtttg catagtgaaa attattagtg 400 <210> 68 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 68 gagttgcacc aagaaatgca aacttattgt tgagccccaa ttcataacac ttggaagtaa 60 taataactat ggtccaggaa gttggtagcg agtaattaaa cgactatggt ccagatcgaa 120 gtcatcgaac caatcccagt tcccagacgg ccagagaata tgcagctctg aaaaagcaaa 180 aacacgtttc gacttgtcar tggtccataa tatataccct cctgtcattt tgacgtagtt 240 taattcccag tatatataaa tccacacaca acggaatcat cctacaccac cagaaaccct 300 cacaaccaac caagagtcca agacttgatc tctgccatgg ccaaacctcc accggcgagg 360 gccaaggctc gctcggttct cgccggcggc ctgatgaacc 400 <210> 69 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 69 atatttttca tctctttttc attgtcttgt acaaataatg aaaacctact acttaaattt 60 ccgagtttag gcatttcggc ggacaatgat aattgctatc tgtgcattgt gcatatcaag 120 gaaaagaatg gtcttcttca agaaattcac aatgaatgag tctgatattg aggaattcat 180 aaggattaat ggatcacttr cctcaaagcg gtacagttat ttaaaagtga aaaagatgac 240 aaacacattc aaagatcaga tagggaaagg aggatatggc actgtataca aaggaatcct 300 acctgatggc cttgttgtgg cagtgaaaat gctaagcgga tcaaaaggta acggagagga 360 ctttataaat gaagttgcta gcattggtag aacttctcat 400 <210> 70 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 70 taacgcaaca atgtctagtg ctgatttggg aattgccgac tgcaccagta ttccaaaaat 60 aagctaagca aagagaaacc atcaactaca acagaaaaag taaaacctaa agcactgcaa 120 cttgacaaaa gttggacatc ctcaatcgtg gtgtacactg agtacactga ggtacttttc 180 tatattaaat tatgttgcay ataaaataac ttatattcag tgttcataaa aaatacaagt 240 gcactcctta atgtccttca atttttactt gtattaacca aatgctagtc tgatcaagat 300 aaatgaatag agaggagact tctttttgtt tcttgggcat aaataaatac acagcatctt 360 tggtttctca acttatagga ctgacagaaa catgttacct 400 <210> 71 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 71 ttgggcagtc gaaattcacg ttcatagggt gacagatcct gagttttgga tccataaagg 60 gtcgatgagt ataacttcat caacataggc tattttttgg atcctgaatg cgggagcggt 120 aaaaccacat cgatataggc tttgatgttt tttggggcat tgggatctca gcttagattg 180 gggtctggtg cttttggtgr tggtgtggca ttagatgttt tgttccttgt cactaggcga 240 cgacgacgta tgtattgtga cgatgaccca tgaatttcgt ttgcatttag gccagctaga 300 tcttaaattt agttaatgct tttattttct agtttaattc caataattcc tttttgttaa 360 ggtactctag gcaggaatct aaacatgctt tattgtgcac 400 <210> 72 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 72 gattatttgg tttagtgacg aaaatttttc cttaaaatta ggatatctta aaaattgatt 60 caacaatgac agactgaagt tgatgcataa ttaagaaaga aagaaaaaga aaaaaagagc 120 agcaagaaag aaaaatgttc atacttgttg ctgtagtcac agttcttaac aaccgaacta 180 caaagccttg aaacaaaacy atcccaacca caaacagaac ccaataggaa gaagagacag 240 actttactaa agaaatgtcg tctctttcaa taatctccag ggcatctaga caagaatttg 300 ggcctctaga taagaatttg ggcacctaaa tacagattta ctttgacaaa ttgctattct 360 caaatccatc attttgagat ttatatgtaa taacttgaac 400 <210> 73 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 73 ttcataatta agttcctaga acaatcaaca aggcggctca tgtggttgtt ggtgttgtag 60 cccttggtag tgggcgtagt atttggttaa gatttgcaat gatcccgtag ctacgttggt 120 ttctaagggt gtagagtgaa gaaatttttt ccactactga tcgttctcat gttatgtacg 180 taattttacc tcaagcatcy aaatctctca ggaccttgtg atcgattcta cgaaaacaac 240 agtccacaac agggaattgg tagtcttaaa agtaaagata gaatgtaaaa ggatagcatg 300 caagcaactt agcaacatga tcaacctgca tatgcttaat taaataatga aactgagaag 360 aaaaagaaaa aaaaagtttg ttttgtcact tagtctggct 400 <210> 74 <211> 326 <212> DNA <213> Fragaria ananassa Duch. <400> 74 attctgttta aggctttaag ccaaacataa gtagcatcag taatccttct ctttcaggca 60 ttttctcagt aaccaaagag gcacattatg aatgcatcaa aagctttaca atagcaacac 120 ttaackaaac gtaattgaag caatatgaaa accaaaatag atatccaaaa caaaactgat 180 gatcaatttc gaaatcagat tgaggtttga gctaacctgg tgatctaggc atcgtgattg 240 accaaaagag caggcaacgt tcactagagc caaaacaaga atatacaact atggaatcgc 300 agtagcttaa aatgcacaca cagtga 326 <210> 75 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 75 catgaatgaa agaaagtagt ttccatcctg ttcaagtaat gcagcacctc ctacttcttc 60 tgtatatcta aatgggtgaa tgaaaaacaa acagctccaa agggttctag agtaacagtg 120 tcgccagagt tactgatctt tagaaaaaca tacgagaagc aaagctacca ggttaacatg 180 gaatacatat gagatatttr cccattactc gattcaaaca ttcaaattct acactttggt 240 gtaaagcatt gcctagagcc caacaagcca gggactcaaa attttaactc aaaattgaga 300 gaagaaatgc tttacttcca agggactcta aacttataat ccgacatttc agatgcttct 360 ttttcgaaat cgctgtatgg gctgaggagg aggagggctg 400 <210> 76 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 76 tgacatttgc taataataat ggtttgctac taagtactaa caattaatac tagtagcaca 60 tctagttatt gttatgctaa tgtatgtaca attggaatgg gaagtttagg aaatattgta 120 caatatttgt tactgattgg tgcatataaa cagaatacaa gcgcatcaat atgtaggaca 180 actactcttt gttgttcggr aatctttggt tgaaaattcg gtttatcatt ggatgtgcaa 240 tccttatttg ctttcttaga gctttttttt taagtttctc atttttgtta acaaacaaat 300 gcactatcaa gaatcaagaa atagaagaga tatagaagaa gaaacaaaat gggccaatat 360 acccatttta gataaaggat attcagggag gatgagaaac 400 <210> 77 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 77 aactactaaa aattagactt cttcgctttg gcgtcttagg actttttctt cttcgttttt 60 cttcacaata ctcgaataag ataacaccat gtgttaaata ttaatttggc cgatgttttt 120 aagtcaatcc aatgctaatc ccacaccaaa ggtttaatga cgataatttc attaaatttg 180 tatctccata atgaatttty tctgaaagag tgtataaaag aaaatgtttc gtaatttttg 240 agtctcgtcg atttgaaaat cattttataa gagtgaaaag ttttgtgata tagttcataa 300 tttgagtaag ctactaatat gttttttcat atggcttatt ttaaagaaaa aaaaaagacg 360 acaaattata tgaaatgaat gcaaagaagt acatggagcg 400 <210> 78 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 78 aataataaga tcaacccata caaacatttg cttttacagt gccgtttatt ttccttattc 60 ttctaagttc taactatgta tatacgaaat aattttggat cataaaatta tataaactaa 120 attgtggaaa taaaaataaa tggagattga ggctcattga caacgttttt ctttgggatc 180 aagttctctc aacgcatggy accagcacct ctatctctct ccctcaaaac acacaaaatc 240 acatcagaaa atgttggaat atacataaga atttgattct taaacaccac aataaaacat 300 taataggact gaagctaggc tttagatgcc atgaataaag tatatattga ttaaattgca 360 taggaaaaat gcaattagaa atgtagctgc tatctgattt 400 <210> 79 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 79 actgtgagaa tactcaattt tataaaaact tagttttaac gtcctccgtc tttttgctcc 60 gtagaatacg gtcctattct agggtttcac aatatggttt cttccttctg tctgttgacg 120 ctttcatcgg acgagacttt gtggtggcca gatggtggtc gggcttctta ggtcggtggt 180 ggtggatcga gagagggagr ctatccaaag agtggactgt gaggttggtt gatggtttga 240 gatgtagttt ggtttctctc caacatggtt gtctagtggt tgaacatggg caatgactag 300 tctcttaaag gtcctgcttc agacgacgac ccgacgatcg tggtagagcg gctgacttgg 360 tttcagacat gaggccactt atttgggccg gttcttggat 400 <210> 80 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 80 gggtctattt acaatatact atatactaaa accagtacat aaaaatattg ttcataaaga 60 ctcgtaaata aacctttctg tccctatttt ctgtttaatc atgagatttc gtgcgtcgct 120 tatccatatg tagccgctct tttctttctc gctctctatt acccatttat ttacaattat 180 gccattgctc tgtacgttty tgtctttctt cccctgtgag gatcattttg caatcgtaaa 240 aatataagat gtcaaaagtc cccgcagcaa ggtgcaggca ttttaccttg tttgttacaa 300 cactcataac agattttatt ttttgttgtt gaataaaaca gatttgttct aaaaaaaaaa 360 aacttaattt gagtatgaag agtgaaaatc aaaattagaa 400 <210> 81 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 81 tgtgttgtca tgtttggctc cgattagggt ctcatttgtt tggctagcaa gggtcgcctg 60 aaagtggaaa cttaggggac gtttggtgca ctggatttga tttgattaga tatccaatcc 120 tgatctagct tgactagatt ggattgagga tgtctaaccc catatcaaat acaatgtttg 180 gtgaggtgca ctcatggcar attggaaaag cggaaattcg ttgttttgca ttttcttttt 240 ctattctgct tttttttttt tgtcctcacg tctgagatac aatttttaca ataattaatt 300 ttgacatgta atttaataaa ttaaaactaa aaatagcata aaaataatta aaatcagacg 360 cacagtaaat actagaacaa ttataataaa atatgtgatg 400 <210> 82 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 82 atatctaact agtgtatatt tttcatagat aaaaactcaa ctatttgtac cgtatatgcg 60 tatgatttaa agtgaaattt taatttcata acacaaataa tgtagaaaat agtacgtttt 120 tgtttgaaac ggaggatgaa caagtacatg atccatcatt ttacaaacgc tacacaataa 180 ttcatcttgc aaacgtactm gtcttctaca taatacatat gttgatagtg atcgatcttc 240 ccgttgcaag atcacgtatc ttcatattgg tcagttgctt tgtttcattt ggcaaagata 300 gcaattaacc aacaagccat acgaatgctt cctctaacta tgtttggatc ccaagaaatg 360 atgacgaaaa tataaaattg agcattccaa ttatcatata 400 <210> 83 <211> 317 <212> DNA <213> Fragaria ananassa Duch. <400> 83 catatctaca gcatctagaa attgcaaact acccacttga tccaaaggtg tagagataag 60 atgatgactg gaatagtaga gcttgggaaa tcaatgtcaa ctcgagaagt caaggcactc 120 cattcatact aagagttgca aacttaaggg gacaactaca attgccttta ttggtaacga 180 tttcatccac tcggcccctr accacagaag gcatgcttat gaatcaacca tatgtagcta 240 ttttaggcat taatactttg gaaaagtttt ttccgattga cggagatata attgccacaa 300 tatgtgatta catattg 317 <210> 84 <211> 354 <212> DNA <213> Fragaria ananassa Duch. <400> 84 tcctgtggtt tgttttccca ccacacttgc ctggttttcc actgacttgt ttcttaaact 60 gatggaattg cagctaccaa aatcccagca gtacccaaag caacatctgc acaaagcggt 120 atgtattttt tatgtatatt gccatggtaa aacracagtc ttctgtgatt atcattaccc 180 catgtagaag cagatcacta catttcagcg tagtcacgtc aaatattgaa tgtagtatcc 240 attgacatgt ttttactttt agttcatact gaagataatc atattaattg cattaggtag 300 tgataacatt tgtttgaact tgaccgagtt aatacttagg cacaaaatta caaa 354 <210> 85 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 85 tgtataatga aagaaaaatt agtcatgaaa tttcacatta gctaaacaag aaaacataaa 60 atttgaagtc atggatccat atgagaatga tctccaatta ccaagtataa cagaaatttc 120 ctccagaaca catactctgc aactagcatg atctctttat cagtggttag atactgttgt 180 tctagtgtga ctcgatgttk tccgcatgat ggcacaaggc aaaagctgac gagcaataat 240 ttgaaggttc gaaatccggc gctccttgag cctcgatttt taccgatagg gtctggttgc 300 cttctatgtc gtttgagacg gaaacttgaa agactgttag atgtgtttga ttccgatagc 360 tatcgtaaga gccatcccct atatcagcca ccaaccatat 400 <210> 86 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 86 attgcttaat gacacatgaa gactcggtcg taccgctaag gccaatcatc actttgaggt 60 agattgtaat ctcgataatt actctatgag tatgaattgt aatcttttga tattgatgca 120 aaatacttat ctgaaagatc acgatccata ctcatagcgt aatgatcgag attacaattt 180 agctcataaa gtaatgatar gaattgtatg agtcgttgtg tgtcgttaag caattttgag 240 ataatgtgga aaaaaatatg aaagctgtta gtcaaaaatg aatttgtaaa aagaaatatg 300 aaagttatta gtcaaaaata aatttcgaat atgtagctag catgatatgc tttaaacccg 360 cacttccata cattttattt catgatcact aatagtctaa 400 <210> 87 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 87 ctaggtttct gatgagcaat ctggagctca tctttggttg tttgaagcgg cctctccggt 60 ctaagtctcc gttggccgcc actacatgct gcttaggagt tcagtgcctc gcaaacttga 120 tcattgctgt cacaaaaacc ttcaacagca cctggtccga tgtttccgaa atctatagct 180 tcttactcac ctttgctacm atgagtggca cctaccaggt acattactct tttgcttcat 240 gttcgatacc gcttggtgtt ctagaattga atacttgatg ttcatgttga gtaaatggat 300 ttgatttgga aacaggtgac aaaagaagcg tgttcctgtc ttcatcgtat actggaatgt 360 tttcaaggca cgccgctgct tgatcctgca agcaaaaata 400 <210> 88 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 88 tatgaagcta atgcagcagt ttgaatttat tccacaccaa attcccaaat ctgatattta 60 catcacaacc agtctcctac tcgtctgtca aattgagatg cacctttagt tctctttctt 120 gtgtatcagt catctacctt gacaaaaata tggacatctt aattcctgac agtctatact 180 ctgcagatat aatcagaagy tcctatatcc tcttaaacac caccacatct tataccaaaa 240 caatacatgt attctagaag ttaacaaaca attgttctat gattatcata atagcgttca 300 gctaaacaca caagcgaaaa agtgggcaca ctaaacacga aagattcaaa cacaatgtat 360 tgggaacttg ggatacctat tcacacggcg ctccctagga 400 <210> 89 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 89 acaaacaaat ctgttctctc acagggtaag aggtggtgct gaacagctga ttattcatgg 60 ggaacgctag gagaaagtga gagctcacat caatcatttg tagcagctat gagctcaacg 120 tcagcggagg cagagaccaa attaaaagag ccatactaaa gcttatacgt tatggctttt 180 tcatgatgat atgacaaaak ttagatgaaa ccattgtcca gaatgagagc gacaacgtca 240 caataagaaa gttgtgagac tctgaccaat gtttaatata aagatcctaa aacaatttaa 300 acagcaagat ccatcctcac aatacaaatc ctactgaaat cgaaattcac aacttgaagg 360 ccctttctgc atcagttaat tcacaagtac gtagggagga 400 <210> 90 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 90 gagatctttt tgctgccttt attttttggg caattaaaaa aaatgtttca tcaaagacac 60 taaagtcacg tttctaatag taaagatatg ttgagaaatc ttttgtacat cagattacac 120 tttcagagat tattaggcct actgcccaca actcggtggc taaataatac ataacaacag 180 agcttacatt tttcctgggm ttttttgggt cgaaattatt gggccttttt tctacgtgtt 240 ccatatatag cgcagattgt agccattgga aatttagttt atgttgtgta acgagaattt 300 ttcatctgct ggaccatgca ctttagaaat gacatatgga cagttacaaa ttagtgtctg 360 aaccttgtgt tcttgttctt gttatagtga agatagagaa 400 <210> 91 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 91 aattgctatt acaaaagtta ttaatatttt gtcattaaaa gatatttttg gctagtactg 60 gtattacaac aaaatacatt taagagagaa acaaaatact ggtattattc caaaaaaaat 120 atcaattgga tgaagtactg gtattacaac aaattacatt taagagagaa acgaaatact 180 ggctctgcta gggttcgttk tagcaccttc aaacatgatc tccatggaga ttttggcttg 240 gaattgttgg ggtttaaaca acgaaatctc gtatgacagt gagaaagaga gagaggggtt 300 ccccgaaaca tcaatcagat gttgcgtttc taggttgcgc tagatttggc tgagcttatt 360 gatttgggct atttcggtgc cccttttacc tggaaaggag 400 <210> 92 <211> 341 <212> DNA <213> Fragaria ananassa Duch. <400> 92 gcttcaagag ttccaacatg ctcgctcaac tcatgtagca acacagcaac ttaccaaggc 60 aacatgctct aatggccatt ttgtagctgc ctttgccaat tcagttcctc ctcttaatta 120 gttcagccaa acaggtagag macttagcta tataagacag ggtttggact ttctccaaca 180 aatgaagcta actgaggctt tagtggaaac acacactttt ctgcagtccc agcatatcga 240 acactggggt tctgatctct ttagtttggt agtatcattg acgaccttaa atttgctcct 300 gcacaccttg ccagaagtct aagtttctta tgcgcctaga a 341 <210> 93 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 93 gacgtgcttg ccttaatgtg taagtactct gtactgttgg tgattctttg gcaggaatat 60 tggatttgat tcctgcttta tgagtcaagg gcatagctgc acagggggtt gtataattgt 120 actacgccta tgaacaaata ttatgtaatg tatatacacg taaatgtttg caaactgaac 180 gaaaatatat atacactagm aatggcaccc gcgcgttgcg ggttttgagg aatccacatg 240 tttgagaacc aatctttaaa agacagaaaa tttagttgat atgaatcatt tgataactgc 300 atgcatatta gttgtctgaa tacacacaga tacagatgat gctgcagtgt ttaaaagctg 360 ttcaaggcta actcggttac aacagcttgg tggacaactt 400 <210> 94 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 94 tatgattgtc tctcacgcta gttatatatc tctcatgctc atggtactgc tttacgtcaa 60 aattgctagg tgaatattga tcgctttaca tagcctgtta ctgctttcat tattgttaat 120 tggtgaatgt cattagtttt ggcatataag ggttatctca ttctataaca cgtacaatat 180 ttaggtatac aatcaattay acgtacactc aatattttat tttaaaatac aaaataaatg 240 tactataact agaaacagtt tcagtcctta ttgttgtccg tgtactttta ctatgagcat 300 ctgacagctt ttttgattaa tagatcactg gacagtgatg agtgaaagat gcagatgcac 360 atcgatgaat cgaaaaagaa gacctgaatg tagatcacca 400 <210> 95 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 95 atttgaattt agggtgtgta gattttacta gtcttgtttt aattcatgga taattttaag 60 tcgtagccaa acacgaaggc atgctaactg atgctatctg ttgcaatatg tgccaagaga 120 cacttttcgg ggaagccctc agttggattt atgagttttc cgctaattcc atttagtatt 180 tccaacagct agtgaatcgm tttgtagaac gtttcatatt gtgtatcgat agggaaaata 240 cctacagttg ttcaaagtta agccgaccgg atgttaagga agggttgaaa attttcatga 300 actaatggca agcggctacc gcacgttagg atttttgaca ataacgtagc agaggaagcc 360 tttaaacaag ctttaattgg gaaaattcat gtacgcggta 400 <210> 96 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 96 aaggcataca aactacatat atattaagat gaagaaagct agcttcggtg tgaaaattag 60 ttttgataat ggcttaataa tggtcagcgt tggaatcgtt taggtgagat gttcatattc 120 ataataataa gatttctatt attagaacgt tgacgcgtaa tttggaaaca tttgaagaaa 180 agaaaaatat aacaagcaay atccgtatac aagctaacta tagggacagt taatccattc 240 gattttcctt tgtgattagg tgatcatcta tcaaacattc accaactgca aaatatcaaa 300 tctatcaatt tagaaggaac tgtttttggt ggcaaaacaa attatcctac gtaaaacaca 360 atttaaactt aacacgaacc atcactttaa tcaagtactg 400 <110> Republic of Korea (Management: Rural Development Administration) <120> SNP marker set for identifying strawberry cultivars and method for identifying strawberry cultivars using the same <130> RDA-P200044 <160> 96 <170> PatentIn version 3.2 <210 > 1 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 1 attttatcat ttgataatat ttgaggtgac tattttaacc cgtgaaacaa ttaatgatat 60 ttttattata tgtacattca aattttagtg ttatttgtcc tactattttc atcaggtcaa 120 aattaagaat atgatatgct tctttttcat ttccttcaac tggtgctgtg taattttgtt 180 acaaagaaaa ggatctagcy atctaggcat ggtatcaaat tgggtcgggt cgaccctcct 240 aaaacacatt gggcaccctg atggtcagcc aacccactaa gtctattaac acataggcca 300 ttgctgggtt gggccgttct atatttcttt aggagtttta tttacgcaaa ctagctagta 360 ttcaagaatc ttgcattcca agcgactttt tttttttttt 400 <210> 2 <211> 294 <212> DNA <213> Fragaria ananassa Duch. <400> 2 caatagattc cacataatat tctcttatca cttgttgact tctccatcac ttctgaacac 60 cgtccgtccg atcctctcac aaagctcaca tcgcgtcaca cccaatccct tctctcaccg 120 acccaatcta aagcaagggt aacatagtca ttgcacccag cccatcagaa tcgacagaac 180 cagaccaaag ctaaaaacay caaccagccg ctgacagcaa ctaaccaacc tctcgaaacg 240 acgtcgtagt tcccaccgag cacgtaaacg acgccgcttc ttttgtcctc attc 294 <210> 3 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 3 tagttctatc ccatgtagac gtcgagctct ctgtcgtatc agagggacaa tgtcataaat 60 gatgatgatg aaactgctgc ttctcctatg tcacaatgtc ataacatttt gtgaagagga 120 tgaagctgca cgtgcaatag ctaaatgttg aaactgttct ctcagactca agcaagcttg 180 gtgcatgaga tttggctccm cttgtaattc gatcgtattt gtaaagtgta ggtcttcttc 240 tattcctatc gatgttggcc gtgatatagg gttcaattag tttctctatg ataattcctt 300 ttcctatatt tgaataaaga gtccgagtct ggacactgca cttgcagaat tggtgcgcgc 360 aatacatgca atgttgaaga ctacacccat atatgatcta 400 <210> 4 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 4 tcgttgtcaa ttaatagaat tacgaacctc accgtatgat ataaatgaga tagggtagtc 60 ttagtccatt ggtttcatga atgcagcaaa atggtcccca aattattgta ccacactctt 120 cctctctttt cctatattcc gtctgtgaca cagtccaatt tttagaccta gaaaactaga 180 tgatgagacc aacacattty agaattttta gtttttcctt tcgaaaattt gtttaggtaa 240 gagaaatgta agaaatttgg ttttaatatc aaagttagcc ctgctcttta acacataata 300 cacgtaaaac cctctgtatg tgtttttatt ggccgaagta agaaaccctc tggttgaaac 360 atttatggtt ccgaatgtgt catcggctag atgtcatggt 400 <210> 5 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 5 ttcaactctc tcaaggaatc caactttcca ataatttttg gaaattcctg tagcccaata 60 caatattgaa gatccaagat ctcaagcttc tccagcgatc caatggaatg aggaatctca 120 accaagctta tacaagctgt taaatggagt tcgattaagt tggggattcc gctcaagtct 180 ggaaattctc ttagagatty acaccgactt aaatccatat attttagatt tggcgaattc 240 tgtgtagcaa gaacaggaag aaaatatcaa ttaaccaacc aaaagcaata ataatttata 300 acattgaaaa taaaaccgag tataatattg aagtacctta cacccgttcc aaagccgagt 360 aatgtgacaa ctgtcagaaa tatgaagctt gacaagctta 400 <210> 6 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 6 atctgaatcg ggtgaagaaa ggaggtcctt gggcttacca acaagctatg atcttgatca 60 atgattacga tggcttttgt gatatcatgg ttgtcccatt ggattttgtg tggatatggg 120 tggaaattga gggacttccg gttgccctga tgacggaggc gacgactaga cttgtgggag 180 agaccattgg taccgtgctr taagtcgaca ccctgggttt gaacagaggt gtggctaggg 240 ttcgtattat tctacttaat aaccctgtaa gatttgaaag gagaattagg gtttcccctg 300 aagatgttat tctggtcaag taagtataag tgactactta gccgatatcg tatttgctgg 360 atgattaatc atggaggaag gtcttgtcca tgtgaagctg 400 <210> 7 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 7 agggtgttaa caagtttaag agtagcaaaa ttaccgatgg tagttatagt gcaaacctca 60 gtctcacaat taatcagaag caagctacaa attttgaaga agatattaga tggctgtttt 120 tggtccatct acagcatgag ctagttgagc taatatgtca atttgagcta gttgtatata 180 gaggtcatca ttaagcatam aactatgacg aagtatgaag tgcatgtctc aacgattaat 240 agcactgatg agagaagaca aagaatgaca attttgaatt ttcaagcata atcaatcgtc 300 cacaaatgct cggattgatt tagaacatca ttcatctaaa aagaaaatag aataatttct 360 agctagagta gtaaacgtta caaaatgcaa aatgcagtag 400 <210> 8 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 8 taggtttgga tgccaacaac actacctatg ttgttgctta tgcaatggtg gaaatggaga 60 acaaggattc atggatttgg tttctatgcc ttttgcagaa ggatctgaag ataactggag 120 agggaaatgg gttcacgtac tttcatatct gataagcaga aggggttgct tccagcatgt 180 gaatatgtcc tcccactagy tgagcacaga ttttgtgtga ggcacttgtg gactaatttt 240 aacaagaagt ttccagggaa agcattgaag gatcaacttt gggcaattgc caaatcaaca 300 acaagggcct attactggaa agaaatgact ttgttgaatc agatggacac agatgcttat 360 gattggataa ctggtaagac tggtcaattc atgtgtgatt 400 <210> 9 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 9 agatcatcgc tcccaagcaa aggcacaaaa ccctaagtgc gcacctctcc aaccaaaccc 60 ttcgaactcc atggcgttcg cccatccaaa gctaagcctc tctccatcat catcaacccc 120 caataagtcc tcgacctcgt cgtcgatgac aaagacccca aacaagtatt ctgcatcttc 180 atccatggtg caggcctcgy ccattgataa agacaccaag tcctttaccg cctcgcttgc 240 gcttgcaagt tcaaaggtgg ttgatctctc tgctggcatc tttgggctca aacgacgtgc 300 tacatacttt tttctggtag ccaagcccct caactttgag ccaccatctc ttgtgaacat 360 gaaaatgttc tgtaatagag tttgaggtat tggagaggaa 400 <210> 10 <211> 278 <212> DNA <213> Fragaria ananassa Duch. <400> 10 tctggtgtga tatttggctt gtattctagt atttgtcctc cccaaaacat ttgattcatg 60 ttatatttat agtcgcartc ccatcgatta ttctgactaa tacacattat gcgcgcttca 120 ttcacaattc atacacgaat ggatcctcaa taccacattc tggacatcat gggaaagtgt 180 ctttagtttc ctcttgttga ctagtacttc tacttcactt taaccacaac ttgttctaac 240 cgtaactcta tttgcccacg acgcaatttg actgaagc 278 <210> 11 <211> 400 <212> DNA < 213> Fragaria ananassa Duch. <400> 11 gttgatatag atagtttctg atagataatt gcttcacttg ttgaatgcat cacacatcat 60 gatatgatta taacctgaat atcctattta tggcaaagtt tattataact tggacctacc 120 cctacccctt tctatccctt cacagctaca tgaagcaaat tgttcgtaat ccttcctgct 180 aatataattt caacacaacr tcaatttttt atggtttcgc tggccattcc actaacctgt 240 caggtttatg tatagaacat tgcaaacaag tttttagaga tcagaaagta tatttaaagg 300 agtttaaatg gtttaaacat tataattatt aaccatatca gacatttact attactattc 360 aaaccatatg cagttatcca tctcatactt ttggttgtga 400 <210> 12 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 12 catcaattat acttttgatg gtgtttttgg ttttcctatt acatgctcgt ctatggaaga 60 actcagagtt gcgatctcca tctttgagcc aagtaataat gcacgatcgt tgtctccagt 120 atttttcttg cgccgccaag atcttactga gctgaatgtg aagcagtttt ttctcttcat 180 actggtgtgg agaatatggy tgccgcatca aatcgttcag cttttcttca ataattcttg 240 cttccttcct ctgtttgttg aaatcagttt tatgccattc cataagctta cttcctgcag 300 ttcgaatctt gtaagcaatt tgtccaagca tgtttctagt agtaggttgc gcccacgctt 360 gtttgatgat agattctgct tctgcttctg ctcgaccaga 400 <210> 13 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 13 aagctgaaga gtcccacatg catgcaagtg aatcaaacag tataaagagc atcatgaata 60 attttgcatt ccatcttctg aaaccaagac ttttgatgtt gtttgacaag caatgatttt 120 gataagcata tttatgggct gtacccccta gcatctataa accttttgga tcttcttaac 180 caaaaagata agactttcar aacaaacacc tttcttcatc aaactggata ttgaaagagg 240 cttaagctat tcagatgatt gttaacttgc tattgtacta taaacttttc tttaattatt 300 gccataaaag ggctcgagtg ctattgataa ttaatgtttt gtaacggcca aagaacaaca 360 aagtaactag acatccccat ctagaaaact gcacaaaaat 400 <210> 14 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 14 cagatatgaa tgacacattg ctctgcactt tcatcagttt catgtagata atgagtccaa 60 gatcaatcct gctatagtgc tattatcttc taagttgttg tagggccgaa ttgctcatct 120 atgctgctat atatttacca tgtatgcata aacaactaaa tgaattgcac tatgtgctgg 180 gattattcag cttatgattk gacttcattg atgaaggttt ccttttagtg ttaggatctt 240 gcttagtttc tttcagctac tacatacagt agaatattat gggattaatt ccttcataaa 300 tttttctaga ccgttaatct aacctttgta tttgcagagc cattggttgt tggaactaat 360 tcttagcaaa tcgtctcaac aaggctagaa ctcaagatcc 400 <210> 15 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 15 atgaagtagc aagttgcagt atcaatgagt ggagagatat gataaaaaga agcccaacaa 60 aaatgcaata ttgcaagatc atatgcttta cttgtcaaaa actacaaaat ccaaatgttg 120 gtattctaaa gtatccaacc acattgagca ctcaactagg cataagaatc aggtgtcgaa 180 cagtaaggct aactaaatam atgacggagt gtatgcaatg cgtacataca tgtgctgtgc 240 attaaaatac acacaactac tgaccacatt gtagctaatt tcatgataga actacttaca 300 agtacctatc tttctccgat tcacatacat aaagcttatg ttcacccaat tctcacaaaa 360 caagcatacg aaacacgaga gggcttaggg ttacatacaa 400 <210> 16 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 16 aataagaaac tgtttattgt tcatcgatac acactgccaa gaatttcccc accatacacc 60 aattgaggcc atgcctgggc caaggactct tccttgatct actagaaatt tcggatttgg 120 ctttcatttg aatcaatatc acctataggc atcgactgtg ttcatattcc tctctggaaa 180 catgccaacc atttgatgak gagaaggcac tgaagtgttg ctcaaacttg acatccctgt 240 tgtggcaggt atattaacat tctcaggtgg aggtaaaaca gtagacaaaa acctactatt 300 aaaaccaaac ttaccagtga atcatcaaat tatgaaaagg aggcacaggt catatcccac 360 taacaccact gttaaccaac gccaacccgt ttggggaatt 400 <210> 17 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 17 catttccatt gtattccgta gcctgccatc atattctatg gattgcaatt ctccatgtga 60 ttgattgtca ttgatggtgg ttttactggt aaactttgat tactatgttt tgttatggag 120 aagactgcca agatttagaa tttgcattct tcattcctgt gtttactgtt gtatatattt 180 ggaaccctga taaccccgck gtcggttcca tttattttaa taattgtgga gttacttgta 240 atttctgtct tggtcttacg gaaacagaga caaaaaaaac ttgtatttac aggtgttttt 300 ctgtgttcag agtatttgaa gatgcttaag caggcagaaa gaagctctgt ctttgtattg 360 gttactcatt ttgagggcac ctacttcggt gcattatatg 400 <210> 18 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 18 ttcttgaatg atagatattt aaaatcatcg aagggtaaat cattgttcat gtacttggac 60 ctagtaatca aagaagttgt gttaacacca acagcaaaaa gatacaaatg aaaaatacat 120 gcatgactct attcaacagt ctagacaaac tacttattaa tcaccaccaa aaaagaaaag 180 tttagcgctc atgaaagagr gagcaaggta tgaagattat tgcctgtgtt gtttctagtc 240 tgctaatctt tttcaaaaac tgatgaaaca cttactgaga taagttcttc ccatgtgtag 300 ttgctttgac tacacgagaa ctcgcaccca cagatttaga acccagttgt tgattgatct 360 gctttcaaca caagttaaaa agaaaaacta cgtaagatga 400 <210> 19 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 19 ccattctatc cgtacaggat gcccttttgt aatataattg cgttcatgat tcagacagtt 60 tactttggag cttaagcatt tgcaggattc atttcatctg taaaaatcat cagtcttctt 120 atttgggaat caaatttcga tctgtgttga aaatgtagat atttgcccct ttcttagaat 180 ctccttttgt atttgattcr catgccaaaa atcccatgtt cttcgttgag aatatgcatc 240 tgggtacatg accggacgca atgcaacctg aatcgcttga gccatttgcc ggattggttt 300 actgattagt cctatttgtg gatcggtttt ctacctgtct gttttgctca caaacctttt 360 ttgttgtaat tgatcggtgt tacccaaaca tgtattgttc 400 <210> 20 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 20 aaataagaaa ggcgaagtat taaaacaaaa tatttacatg cacgctcctg ttcctgagct 60 taatacccaa ctacccaaca aagaacacca aactgtctca acacaaatga aaaagagaca 120 caaacctgta aagacaaggt actcctgaac cccaagaaag atacacccaa atcagcatag 180 tgctttgtct gcacagctcy tttaaaaact tcaatgaaaa caacataagt acataacaca 240 acctgcaata caatccaatt cagagaccat gaactgtagc attttcacat accgcaataa 300 tgaaagctag cttgactata atttacaaaa gctcgttatc agtactaccc tgaacagaaa 360 cttagataag ttatttgtct ttatttttct ctcagtttcc 400 <210> 21 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 21 gaactcacga tcgacctatt acaggataca agttgtttat ccgttaaaat aaaatctaaa 60 aaacaaagtt ctgaaattga ctaccaccgt ttaaattcct cattgtgttt tttaaatctt 120 ttcacacgca agctgagcta actggtaata gtattgtatt gtagctaaat aatctttaat 180 taatgctaat ggtgcaggtr ggactattct gtctcttatt tggttggata aaagaccgac 240 gtgatgatca aaaacaaagg cttggagact attccaaagt catgaaaacc atagacaaga 300 tatgtatctg tctccctttt ctttttcttt ttattatgaa tgaaaagaag cgaaaattat 360 aattacaaca tctgagttgc aaatagcaac agcagctgtt 400 <210> 22 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 22 tatgaacact gaacagttga acacagtttt ggcacataat atggggtcaa ataggattga 60 agatgctgtt gatggatttt tcattcaaaa ggagtggaat caaggtagat gagacctcta 120 tttctgttta ttgatgtgaa caaggtctta aaagaatgtg aattacaaaa ggtacccaat 180 tcaaggacct gcagttgcar tatcattttc agtagcttgc ttattattct tttcattgtt 240 ttttcaattg ttaggctttt cttgcttaag atacatctct atcgtgtaaa tgcgtaatgg 300 tgaagcaggc atttctttct ttttctgagt cttttctcca tttttgttta gtggtgtttg 360 caagctttgt tcatgttttg cctctatggc tctatctatg 400 <210> 23 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 23 gatcatatcg actggggatc tggaccgacg gctcctgcgt aactggaaac ccaatgggat 60 caaagaaact tgctcttttt ttcttttttg taataataga aaattctatt aaaagaaact 120 tgctctctac ttgccaagaa atgcccaata aaaatctcaa aattaagtag ccatgagttc 180 tcaatcactg tcagctgtay gtgtcgtcta gctactttca aaagatatat gaaagaaaaa 240 gaagaacaag gtgatgatga gatttgaatt acccgggttt cggggtttta cgtgtttaat 300 ttattgaaca cgtagcaaaa atagccgagt aaagataatg atcaggtagg caaagcagaa 360 atatggtcac agatcgagct gaccatgcat tccgtgctag 400 <210> 24 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 24 gctcgcaaga gatggatcgg tactcgtacc agagtatata tcaggctgtc cagaaattat 60 ttgcataata attattcaat tatattatcg gaatcactat gcagtgagct aaccacaccc 120 caattacgtc cctgttgacc tgtgtttggc ttgtccaaac atataccgta cgtacagctc 180 cgaagaaata tattgagatk gtgtaaattg ttgtttatcc acaaaaattt gaccacaact 240 acaaatgact gaaatgagta gaacaagatt accaagaacc tcttggtatt ggccatgggt 300 agtctttgca tggtaactag atgtatcgga taccctcatt tacaactaat ttgacaaata 360 ttacaattta cttgaaccaa attatcattt tcaacacaaa 400 <210> 25 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 25 ctctaggcat gcagctggta cgtattatgg tgggagctgc agggctgtgg aatgttgtgg 60 tagctagttt agtcgaggcc accatgggag cacaaattga aaggctttaa acggatcagt 120 aactgatgag aacttttgag aggtttcagt tgccgtcaat gtacgaatat tgcacatgag 180 gatgtgcaac gtctcagatr gttgttcctt acgccataga tacaatacat cttcatcatt 240 tgactcaaaa gctagaagtc gacatctgga aacccgaaag caaggggtgc agccgtgcag 300 gtcgaccaac tctttggaac atcattcgtc atcttctttc aactcccaaa tatagagact 360 gagtctgctt tcaggtcctg aatacaactg gcatagcctc 400 <210> 26 <211> 395 <212> DNA <213> Fragaria ananassa Duch. <400> 26 aaaaaaaaaa aaaaaacttt gtatgaatag ttcctaaaaa ctaccagcca tatgtactga 60 gcgcaaacaa aaaaacaaag cagactattg acatttctac aaacatttga ataggaaatc 120 acaagcaatg ggatgaacaa tacaacaata caaatatagg gacaacagca gaaggagatc 180 attcagaaac atgamagtga ttgctcctct tgggtccaag ctaataagca atgggagatc 240 atttataagc taaaatttag gatttctaat caagggtatg aaaattgttg ttcaatagat 300 caacctcaat agtctatttg tctcctcagc attatatcaa cagccattaa ttactctatc 360 tctttaaagg tccatcgatg tcaatcatca tatac 395 <210> 27 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 27 tgcaggatac tctaacgcag catggagagc agacccttca caagccagtg gtggttatgg 60 tagttctcag tccaggcaga cttaacagca gaggtccaac agagttagaa tttctttcac 120 aggaatcctt acacatacga tctttcccca acctaaactt atgcatgaca agagttgaca 180 atgaatcttt gggctggtty gatcgctcga ttcttgatgg tagttggcag cagtatttct 240 gctagaaatc acgactatta cattctagca gtattatttt ttagtaaaat cactttaata 300 ttagcttgct tagactgtgt gtgtgcttgc taattttgga gctaccctca tcctcattca 360 actttctctc ttccccttgt tgtttacact agatatttct 400 <210> 28 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 28 cagagtgatg tattggagat ggtgaggact aaggagggtt ggttgcgcaa cgatctgggt 60 tttggaagta gatggagggt cgtgggattt agaagaggta gagagggaga tgagggtagt 120 ttagactttt ttttgtcaaa tttagtgtca aagtcggctt gtgatgttat gttttgagtc 180 aatttcgaaa tttggacgar actgatagaa aataaaagta caaggaccat gctgattaaa 240 aaaaagttta tggaccaaat tgattttaag cctaaaattt aagagggtaa actgaattta 300 atctatatca aaatcacctc acgcaatata atcaatcaaa catatgcatc caaaaaaatt 360 attcatgttc cacatgtctc aacataatca caactcagtt 400 <210> 29 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 29 caagccctat gcatgcacat tgacactagt aattaatcgg tggaaaaatt atcacatggt 60 cctgaaccat ataatatatt acatgcggtt tttttttcaa acaaaatttt ttattaaata 120 aaaaagatag tacacatggg gaactgaaac taaaacccct tacaaacaaa caatagaaca 180 aaagacaatc aaaacataar aacaatcaga gctgattgta ttatagatca agacacacca 240 cgggtgtccg attacgggca tggtaaaggt aatatagtac aggttgtttg tttctcatcg 300 tctcttaaac ttaatttctt ttctaatttc cacttcttca tggtcctcac atgattcagg 360 tgatatgatt ggctatgacc atcacatgac agtgtcacgt 400 <210> 30 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 30 atctctgtag gtttggtaag ttgagttctt tttttatctt ttttgcttat tttgtacttt 60 aaggaaacca gaatcacaac agaaccagaa agaatgtgtt tctttcctct ttgaagcgat 120 gaaacggcgg cggatatgtt aatggtaacg tcagattagc ccacgagaat tttaatttgt 180 tattggatat tttatcttam caatattgga tttgggtact caaacttgcc accactccca 240 ccagctctac aagaatgaca ggaagtattg ttgctactct agcttcaatt aacatggtag 300 attgtaacac ccttacatct atgatttgtg tctcctatgt acgaatgaga ttatttgttc 360 gaaaagatca tttaacctaa catacatcga cggaggtagt 400 <210> 31 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 31 taaaaatcac tttctcctct aaaaaaattt gttgacacaa aataaaatat atttttatga 60 tataaatgct agtgaaaact catttggtgt ggtaaaatgt tgtccctaattt aaaacactca 120 c ttacagatagtagt aaacca atttgggttt gtgcgcctgr ggcgaaagat tagtttgact ttcctcgaat cttctagtag 240 acctcggaac aaatgttgac taggtggatg tgttactaac ttactacgta actgagataa 300 cttattacat cactcccaag aaaacaaaat gatagtggta tgatgtttca caaaatatga 360 tcttaatttc atgacatatt agatatatat tgagtattaa 400 <210> 32 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 32 ctataaaagg gaagcctccg atgatggaga acatcgagac aacaatgaca gagaagagct 60 actccggcgt agtcgtcagc gaacccgccc cgagttccgt ggagcctttg aaccgcaggt 120 ggatgtccga ttcaaaccga ggtacgtatc ttcttctccc tccggttccg gttctcgtac 180 ctcttgccct atcaattctm acctatttcg ttttcggagc agaaaagttg agagaagctt 240 ggccgatcgt caaatcgtta ttggaagaag tcgggatttc atgctcgatg gatacggtac 300 gttcaagtct gtgaattttt ggaaagctag ttatgaaatt gagattgaaa tttgaatggc 360 acactcaggt tgattccgca gtcacagtgg agtcactctg 400 <210> 33 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 33 tgaaagcctt ccgtcctcca ggtgaagcgt atagaatgaa tctaatgttt tcttctgata 60 cacagaaaaa cactcttact gctgtgggtc catctccaag actaggctgg gtcaggaggg 120 ttttgaaaat ctgtataact gaaaagctga gtccctcgca tgttataagt gtgttcatat 180 gcaaccatga tcagagaaty tcatttgttc ggaatgttga tgtccttaat gtcaaaaaga 240 ctggccattt gatgatcaaa gatggtgcag ctgaagtgat gttgtccttt ctcgaaggac 300 gaggtacatt catctctgtt cttgttggtt gctcagtatg cttggaaaca agcatttgga 360 ttgaaatgaa gaaaacatga taactaagtt catacgcgca 400 <210> 34 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 34 gcccccatct cttgagcagg tgtgttatag gatttgaaga attagtttta cataaatagt 60 aaatatactt ggtgttataa cccaaaatat aaattaattt tctttcactt cttcagctga 120 agctgataga ctgtgtcaat ggtgttcaat ctcttcctcc tcctggggag caagaaatgg 180 tgcgaccgca agtaggtccr attgaaacac ggggagttct tggaggagga cctcacatat 240 acccgctctc cagttgggga ccgcttgatg ctctgctgtc tcaattggat tactccttgt 300 ggtgttgcgt gaattttgag attactgggg tgtggccatt caatgatcga ttctagctaa 360 aaggtttggt tccaatttag tgcatttcta ctctgtctat 400 <210> 35 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 35 gatgaagaac gaaacaagcc ccaagaactt cctgtccaag ccttaacact cactcatgta 60 tatattgtta taacaaatga acacttgaaa tgctagacct tagatagcct gcttttatag 120 tttggatttc aagatctggt gaggggaaat ttagtctaga aaatctgctg ttatcgactc 180 ttgataaaaa tggcaatcar ttaagatttg ctcaatcaat cttctgagtt ctcttacata 240 tgccgcacca ttaacatttt ttttttcttt ttggttcttt attcatgaaa cttaattaaa 300 ggacatttgt ttttcctttt gcaaaaatgg tttatacaag atcccaataa agcaaagtta 360 tcagagaatg tgatggcttg tttcataatt ttagattaac 400 <210> 36 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 36 gtcagaggtt tggatcccgg tcttcgattt gctatggtat ggggaatcat caggttcttg 60 gcgttgtggg gaatgatcta ggaggttttg tgagagcaga tctgggtccg atcaagagga 120 aggagaagat ccagcgtgtt cgggttgtgt tggatgtgcg aaaccagatt ccagcggaaa 180 atatctttaa ctcctcgtcr gagatttctg tcaagctgag cctcaaggtt gaaagagccc 240 acaggatttg cagggagtgt ggcttctttg gccatggagg atgtacatgt gataggggtt 300 tgatttgtgg gttgctagtg gtggatgcag tggtgaccga cggagagctc gagattgttg 360 aggagccaga gatgcaggcg gaggaggatg aatcagaggt 400 <210> 37 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 37 tcagttggat gttttctcca ataatttcta cacttctttg ctcgatcaac ctcaatggga 60 cctagcttga caaagaacct acggacccca ggggtctgcg tggcttggga tcccaacacc 120 tgtgcaagcg gccataatca taaacgttct cctagctaac agattggtgc ccatggacca 180 ccctcaagct ctctgggatm gaaacgggta agcaattcat ggttgagtcg agatgactca 240 gatcacagac gactgaataa ttcacaagcg agtttcagat gtaccccaaa tgagttgctt 300 gatttgaggt cactcggcat atttattgcc aaaacgtatt ctctgcataa atgtgcgcac 360 ctgcaaaaga tgttgtgaac ctcacaactt tcggataaga 400 <210> 38 <211> 257 <212> DNA <213> Fragaria ananassa Duch. <400> 38 aaaaaaaatt aggaatcata cattagtttc tccttttata tataattaca tcatacrgtc 60 caaataccat cattttattc gtttcaacaa tcaccagatt atctgctata tattttatgc 120 atccaagtaa ataggatttg cagttcccta agcataatca gacactaaac ataaccaaaa 180 gaaaattagg tttcacctca gcatcgagac cgaaggaatt cctcccaatc acatccttta 240 aatgtgcttt ttctaga 257 <210> 39 <211> 295 <212> DNA <213> Fragaria ananassa Duch. <400> 39 aagtggagac actggttttt ggaggcttag gatttcctct cgctgtcttt aaatttttcc 60 gtattggcta ggcattgaaa aagattgttt tcatgttttc ttagaagaat cacatgatgt 120 gataagtcaa gttgtttact atagaaccct catggtaaga ctttgagtct cgcgatccta 180 actacaaaga gaaagaagay agtgcacatg cactaactca aatctagttc ttgcctcaac 240 tcccacaagt ttgtgactag caggattgag agacatccga gtttgtgcat ctgtg 295 <210> 40 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 40 actagaaaat accttagaaa tataaacaag attacacatg acagtagaaa ttaagcagca 60 acatgtaaat agcttacaag acctgtcaaa agtttgcgca gcattctcaa agatcatcaa 120 tgccctacga agtataagaa gaagaaggtt gggtaatgtc tcgtggagaa acttagcaaa 180 ggctagtggt caaaggaccr attttcgaac tgggcgttcg accttcttgt agggatctac 240 gaacagaatt ttcaccgtag acccgttgtc gaatagaatg cgactgacgt tgaagccatc 300 gataaaggcc gtaaccagaa atggttcgta gacgactcca catccttcct cctcgcagaa 360 tgtaataggt gcgcacccct tgcgctgggc tttaacaggc 400 <210> 41 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 41 tctctgttgc cgccaccaca aggagagcct taggcgaagt agcacgacag tttgattcct 60 tggtgcttct tgcaaatttt cagacacaga caacatgtta agtcatgcgg ctttcaagat 120 tcttaaccaa aaaatgaagc tagcggggtt ggacgtagtg gacggtgtac ccaagagagt 180 agcaagtaaa gttcaatcgr acatgcctgt tcacacccat tagaaaaccg cttccgggga 240 acctcttgca gatcacaatc cgcttgtggg ttgacgaggt cgctgaccaa gcttcatatg 300 aagatgtcgt tgtcaccaaa cagaaaggta aggatcatat tgtgacaccc ggaaatttga 360 caagcttaat tagaggtcat ttggtaaata gtttattgac 400 <210> 42 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 42 caaaacatat tctcaagaaa actatatatg gactcgggga gtacgactgc ctccaaaccc 60 aatgagtgca aaccaaagag cagttctccg ccgcatgcag tctcaaaaat catagggctt 120 ttagcatttt catcttcaca tgttagagag ctagaacaag agaaggagca cattaatttg 180 ccaagtcaac ttagttaggk tataactcat aatatcttaa ctttgccaaa agtgaaacct 240 caataccact aagtactaaa tctattaaac aaactaatga tgaatgagta tttgtatcat 300 caaacaaaga ccaacgacct tgcatctgcc tccttttgac aataatagat caccagctgg 360 tgaatctttc tacctaaaaa cacaagtgat cgaccatgca 400 <210> 43 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 43 acataccaaa gcctaagctc taaccttaat caatcttttc ccctttttca atttccatat 60 tactgtttgt tttagtgatt cataattcta attaagataa tataatccct tgttattgtt 120 aaatcctgct tttgcaagat catttgtacc tttatcaaag cagaggcaga catgataagt 180 gtttaaatat caatttgagr aaatttccca gccatgaaac accagatgaa ttgtcttctt 240 aaggtaactt ccctggaata ctacagaatt attgtttcta ccggtgtttc atcatggttg 300 cagtgactta ctattagcgc aggtatgatc aagtagaaga aataaaggca aaaacaagct 360 actcggttcc aaatgatggt attggtacct atagaaaaca 400 <210> 44 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 44 ttgtttgatg tctctccttc attgaaatcg gaagaggagt tgttctttgg gaagaccaaa 60 gggtttcaca gaacctgtgg attatccatg cacgatgcta ctggatgtga tcaatatttt 120 ctaatagaga tggatgaact catgaaggag gcttttggtg gacacactgc tttgtatcct 180 tctagttcca gtgaagctak aactgatgtc aagttctact atactttgga gacggactct 240 attgatgaac ctattgatat ggttttggag aaggtgcatg ttctattgat gaccaacccg 300 aagaagggta ctttgcatga gatgtccccg acgagggagg tcgacaatat tttcacggct 360 aatgctacct tggacgtcat tattaggttg atactaatcc 400 <210> 45 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 45 ttcttttgtt ggcttaacca cttctttaat gtcttcctaa ttgagaacta gctaggagat 60 catcaactcg atcacgaggg ttggcacatg acttggaatc atgccccaaa aacgcgttat 120 attaacaaga gaatcaacaa acccaaaatg actactcaat attcaccaat taccatctcg 180 gataagaaaa ctcgaagcam aatgaagact aggacatgga aatgacactc ttggagtctt 240 ggggacccaa ttttatgctg agacaattct tctatatgat ggtcgagaat aaaggttggc 300 ttttaacaaa gatgggaaag taatggctaa catgactgtt tagctggtga cttaaggatg 360 gcaaagtgtc actgttaagg gcagtgatcg aaagcagata 400 <210> 46 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 46 taacaaacac caacttgtaa aaattactac tctattaatg actccattta ttaggggatc 60 accaagcttt gatgacttgg gaactctata atgacgtcaa ctgcatcaag ctagggaacg 120 gatactccat aatataagtg gaacatacaa gctgcaagca gccaacttgt tctagtctgc 180 aaaagcaggc aagttatacy actaacgact gttgtatgca atattgtata ctactaacca 240 ctaccttcct aaaagaaaaa tccaacgact atcaatagct accttcaatc agtacaaaat 300 ctctaccgca actctgcctt cgaaggacca ctacgacacg ttcaatgaat gagaatctga 360 acacccaccc ctagtcgccg ccttaaaatc aggtctaatc 400 <210> 47 <211> 246 <212> DNA <213> Fragaria ananassa Duch. <400> 47 atagttacga tatatgttaa tgggtagata tatcttgggt attgctgtgc aagttctgca 60 aggatggatc ttgcggagga tttgaacaaa gaaaagcaaa caaattaatg ctgcccagtc 120 tcacctatat atgatctgtt gcagaggatc tgaataacga agtttggaag gagcaggtgg 180 aacccatctt tgaaaagcay ggcctaacct gtagaatgga tgaggtatcc ttctttattt 240 ttctta 246 <210> 48 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 48 tagccaactg aattgatgag gatatcatta ctaagttaac atgtagaagt atatattcaa 60 atcgttgtct aggctgcagc acagccattc aagtcgatcc gcgcctctac gtggcatgtg 120 tagaaatcac gtttgggcta tgttgctgag ttcttgggtc tatttggatt ttggatagtc 180 tgttgtttct tttgtagtty tttgtttgca atgtaattta gtttttagat ttgtttttta 240 gatctcaaat atgtccttac gtttacgaga ttgttggata tatgtcatgg atgctgatga 300 atgtttgaat ttgggtagct gggatgacca agaacccatt tttttgccga ctgttcgccg 360 agcatactat taatctgtgt taagcgtccc gagagatctt 400 <210> 49 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 49 caaaagtcaa ccaagcttgg aggagaaaga gagtgatgac tcagtaatta ttggcattcg 60 gtatgggaaa actccagatt gaaacggatt ctattccgaa actgttccta aaatttcatg 120 gctggaaaca tcgtctaata cataaacgac attaataaag attcaatttt cctgcaaata 180 ccaaaaaaaa taccattgay cgagtgcaac caacctcatc cggaattcca gttttggatt 240 tctattaaat gtgaggcatt atatatacta taaagagacc cagcctctat attagatgaa 300 aggaaatgaa gaaagtagtg ctcctaaaac gctttctgca ttagaatcga attacttctg 360 tctctgcttt tcttgctcct aactgtttct gcagctggac 400 <210> 50 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 50 tcactgttgg catgacatga gagccatgct tggtgttaga tcttgacaca ttgagaagtg 60 cgtaaagggt ctaatacatg tgtcgcatat ctaacctttt tccttgaatc acttttagac 120 catgcctaag agcatgattt gtgtcaaagc tcgacatttg acatggttga tgagggtagg 180 ccgaccatgt ttacatgcar cttttgaatg tccacaagag tgtttgaggt tcgaggctct 240 taagctttgg aggtcgaact cttagtctta gattttgagg ctctagactc gtcttgtgtg 300 tttagatcat gacatgtttt tgtcttgcca tgtcagatgg tgaggtaacc acttggtgac 360 actccaagac ttatggtgga ggtccaaagt tgacaccttg 400 <210> 51 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 51 atttaatgga ttactgtcat cctttaaaaa aaaattacat ctaaaataaa cgaatcatgt 60 ttggttacaa gtttaaatgt atagttaccg agtttctcgg agcagggaga aaattttgga 120 aacctagcct ctaggttttc gatttggcag ctgtgctctc cctcttggtg gacgttgatc 180 gtgggttcta gaggcggcar cgcgaattca ttccaacgac aatcatgtgt ggtggcgagc 240 gaaggatcag tgttttgcga cggcgaccgt gcctttccaa gtgtcgggtt tgatcaagac 300 cgtgcgatca gttatgattg gctggcaacg gcttgatctc tatcagaggt cgggcgatgt 360 agcaggctgt tagaacttca ttggtgtctc agacttaagg 400 <210> 52 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 52 tctttgtccc atctgggctt aattgtcaaa gtttagtttc caaagctttt tgtgcaaatg 60 aactaatgaa agaaaacact tattgttgtt ctggccatgg atgtcaatgt agtttggaaa 120 aatatgtcta agttcgaaaa aaaaaaaaac agaaattgta gtgcgtgact gcttcccttc 180 acattctgga atataattgr agattaacaa agactaaaga agattgattt cctcgtccat 240 gtgcagcata tgctttaccc cgtgggttct atgtattttt tttttaaaga aaattatatt 300 atgtctcatg ctttataaaa tatgtgtcaa tcattgattg aactcacgac aagaaaaaca 360 cactaggcgg gcaatagttc caagcaaccc ataacaaaag 400 <210> 53 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 53 agtaaaatca agaataaaat gcagtagtct tccatcactt gccttgacaa ggattaagga 60 aatcaagtcc tccttgccct aacccctaaa cagactagaa aactagattg ggtgataaaa 120 cccctaacta agtcatcacc gaaaggcttg aaagggataa agtccaaaaa tagcacctag 180 cctaactaga gcccatgcar ccttaaagga tagacttagc ccagacccaa caaggtgtag 240 agactagatt cgacaaccaa tgctgaagtt tgacgttgcc tgttgcaact ctgccatctc 300 gtcagccata gtgcatcacc acaagcctca cataccgcga cactgcaccg ccgccttccg 360 accatgcctc cacgatcagt agcaacttat agatcaaaga 400 <210> 54 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 54 tagctcttcc attaacttga ttgtgtctgg taatgaatat tacgatttac gaatccagtg 60 atctttattg gctttctggg tacaactagg aggaaggggc ctttggatag gaatccaagt 120 tggtgctttt gtgcaaacca ttatgcttgc ttttgttacg acttgtacaa attgggataa 180 acaggtctgc ttctcaaccr ctcatttttt gatgatgctg atcttggtgt aatatatatg 240 aggttgatcg ataaggttgg actgtgaaac tgaatattag ttttttcttt ctttcttctt 300 cttcttcttt gtttcttttt atttgatgtc aggctagtca tgtcataagg tcataagagc 360 ccattagact gtgaatggca tatttaatca tcaatatagc 400 <210> 55 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 55 gagctctgtt ttgcaagatt aactgtgatc gttgttactg attttcacca acaaacaaca 60 agcactttgc ttagttctag gatgaaaaat atttatattt atcataatat gaacgtactg 120 ggagacaaga agagttatga agttatgatt atactcgtga aattagcatc cgtcatgcac 180 gaataatctg agtatcatcr gcaacttgat gcaaaatacg tatatctttt tcattaccta 240 acaaattggt tgtcagtgtg catagagaaa ccatgcactc tttttcattg ctaatctgtt 300 atattatcta tatagaaata aatgcatgta tttatcgtcc ttcttctaat taaaggggaa 360 aaaaatctat aaaaggcatc aaggcaataa tgtgtgctgt 400 <210> 56 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 56 ttttttggtt tggaagaata tcaatggata tgtcaaactt cagctctatt tttccattct 60 ccatatacgg agtaatacca atgtcgagat ctcgtcacat ctacaattgg ctctacattc 120 agaatagtct caaacctcaa ttgaggataa tgaggaattg ttaatgccca caaatgtgat 180 tgtatttccc aaatccattr cctccgactt gttcgaacac cttagaacca tcgcttagac 240 catttgtaga tatttgtata tataatgttt gtctctaact acctttatgc ttaattgaca 300 caccaattat aagaaaacta tgtaagaatg tgtggtctat gtttggtttt ccctagaaat 360 attctttgct atctatgcgt atggctgctg gtatttgttt 400 <210> 57 <211> 265 <212> DNA <213> Fragaria ananassa Duch. <400> 57 gccgtttagt gggctgatca aatgaaatga aaaggctaag atttcatttg aattactggt 60 tcatttccac cttccgcaga taatagttat cttttgcaac gcccggatat tatttttact 120 gttactgtca tttggacagt caacgattat catttttata tttatttaca tttttgaggt 180 ttaagccgag atgactgttm tttaagccaa agggacaaca agacctagat gagaacgccg 240 cctctgttag tactattatc ctttc 265 <210> 58 <211> 400 <212> DNA <213 > Fragaria ananassa Duch. <400> 58 tgtgaaacca ttcaggactt cgaactgtag taacaacctt ctgcaccatt cacatagctt 60 aatgtagtta tcattttcca tttccttgga gaattataga gttgaccggt ccagtattaa 120 cttcattatg gtttgatgtt cttagccctc tgtcagtcat gaagttgaaa tatgtgagtt 180 ctgagtcttt cattctaaay aacattattc ttgacttgac accaacaaat atgtcatgca 240 gctctacaag gcccaaagga atgtaattct ctgctctgca gcatgtcttc tttactggta 300 tgttggttag ctgtcaaatt cccttacatt ttctctgaaa tagaactttt gtgcccctaa 360 ttgatttatt gtggtttaaa atgttgtaca aatctttaca 400 <210> 59 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 59 gtactcggtt tgcgatttga tttttcacta tgttgattgc tacgcacagt agattgttat 60 tgctagtttc gcttccgcta agttgggact ggtctcagtc tttatgtgta aaagaatcaa 120 tttgctggcg catgagttgg gactcgtcac agtgtttatg tgaagaagaa tcgacatgct 180 gctgtattag ttttcgtgck gattctttaa ttatgtcatg aggagtggtt taagagtctg 240 taattttttt gtttttgttt tggttcaata tgagttgtga gtcggatgtt aaatggagtc 300 tcataagtag gctgaagagt gatttcataa gaaaattgat tatctattac tttgttttaa 360 tttagtattt gtcgcctttt atgaatttgg acaaataagc 400 <210> 60 <211> 347 <212> DNA <213> Fragaria ananassa Duch. <400> 60 gtggatcatg gtagtacaga acatcatgac gtttaggatt aggttggatc tcaaaggtgc 60 actccaaagt atcctaaaaa gctatgtagc ttggtcctga actttttatt gcatccaact 120 ttggatggat tattgttgct aaatttygtt gaaaaagcta gctagtgtcc aagtttttgt 180 cccgatacat tttctttttt caaattacga aataagtgtg agtgaaattt ggatcataaa 240 aagaaggaat ttgcgtcaaa aaatcaaagt cgtcaaagta aaagaacgag tttgatgcaa 300 ctttcacata ataaaatctc atctttaaca tcattttgag tctacac 347 <210> 61 < 211> 307 <212> DNA <213> Fragaria ananassa Duch. <400> 61 aagaaaatta tgtgattgga ttgggccact cgtcagtttt gggctttgga tactttgggc 60 ctgaagttta agattacatc acttacatgt ggtcgagttg ataagaygta agacgaaaga 120 tgtggaactc ttataagtta taaccaggtt tgaatcccgc caatactgat tgaagttaaa 180 gaccgataca ttctccacgg ccaaggtaga taaagcattc tgacatgatc cccggtcatg 240 gattagttgc tatgcctaaa atgcttttga ataaaccgtg tgatttcaat aaaaaaaaaa 300 attctaa 307 <210> 62 <211> 249 < 212> DNA <213> Fragaria ananassa Duch. <400> 62 ccagtattcc taggataaca atctgtagtg gggaaatata tatgtagtrg aaactaatcc 60 catatcgaga aggagttaaa aattatgcag tttataagtt aacacgtact cgataatatt 120 tgctgaggcc taaggtgaaa acctcacact tgaaaatccc aaagggaatg ttcataaaca 180 atcaagtgcc aaagtgagga caatatcggt ggtgatcaac ctcctccctc atcaccgagc 240 gaggccttt 249 <210> 63 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 63 ctcataagca acataagcag ctagcaatct atctgacaaa tagtaaaaaa aacgttcgta 60 ttataaatct ccaaatctgt tttgatattg actggagcag ccaaggaaaa agtacactac 120 caagcgcatt catgatatag agactggcat agattggtta atactagcaa actcaaaaat 180 gaatttgttg attggaaagr cacgcagcca cgtcgtagag acaaagcctc ctctcattgc 240 tcagtttctt cttgttcttc ttaagacaaa gcctcttctt tcttcttgct cagtttctgc 300 aaccactcat tccccacttt aagaagattt gcaggttcct cattgtttat aatacagcgt 360 ttcgcaaaga tgaccaccat atcaagttcc aggtcttgct 400 <210> 64 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 64 accttgaaat tttgataaga ttgtatcgaa attcgaaaat ctcttcatga ttcttctttt 60 tatcatcaac ttattcttaa tttcatgtct ttctctggtt cttagagtat tagattacct 120 tcttatttat atttgatcgc atattcgaag ctatgcgtgg agaatgatca atgaggataa 180 gccatcaagg aagaaagtty ttaaaaaacg tgccgctcaa aaggataagc aaccaaggca 240 agaatttttg gaacaacgta ctactcgaaa aaactattcc aaagcagaat atgttggaaa 300 aaagactgaa catcgagcgt aataactttc aaataaaaga agataatata agtaaggact 360 ataactttca aacataagaa aataatgcaa gaagttttgg 400 <210> 65 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 65 taggcacata tgcttaactg cgtacttgga caacacttaa gctaaccata acagccaaac 60 tcacccaaat tggaaacatc aaaggcaaga cagcagtacc agctacaagt agtagcaagc 120 cgctcgtcaa acagagttag gacacgccca tacgcgtggc ttcaataccg tttgacagaa 180 caacaatatt gagggcaccr gtcccacatc aaagaagagg ataagtcacc tacaccttta 240 gcttatagat agcaactctc tctctctctc tctctctctc tctctctctc tctcttcaat 300 tactatgcaa ttacttttta cctactgtta ttatgttaac attaatattg aattaggcat 360 cgaagcatag aagatcgctc atccgcagtc tcttcctcta 400 <210> 66 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 66 taacctcatt acaagtcaac gaaaatctca ctaaatagcg agcatataaa caaaaaaatt 60 tcggtttcct acaacaaatt catattttaa attctcatta accagtcacg caatgttgaa 120 taggatctcc agcaacacta aaaccactgg caagctagat agaatttgtt caacatacca 180 aaattcaaat tatggctaak actagcattt ccattaaatt tgaaacggaa atattttagc 240 taatttaatt atttttgttt cttaaaaatg ggcaggcgcc tatgtaacgt aaaacatttt 300 cacgattgca tgaacaagtc aattgtggct aacatttttt ctttccgtta acagctaatg 360 tttcgttcta cataagctgc caaaacaagg tgctaatcca 400 <210> 67 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 67 atatgtttat tgttgggggg ccatttgaga agcaacatgg ggtcgagtac cctgcagaga 60 ggcctttgcc tctgaagttt ggtgacgcat gtaagtgtcg tgcgctctac tttatatttt 120 atatttatca cttaattcca atattaacta ttattcttgt tgtgtagctg agaattgaaa 180 tttcgctcct caagataaar cacttcaaat atgaagaaag tcttcacgat tgtccttgaa 240 catggccccc tttcctttct cagagcctgt tcaattgaag ctcttatctc gcgtggattt 300 ttgtaaagcc cagttatgca atgtttacct tttttgtttg agcttgactt tatgcacctt 360 tgcttattgt ctggtgtttg catagtgaaa attattagtg 400 <210> 68 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 68 gagttgcacc aagaaatgca aacttattgt tgagccccaa ttcataacac ttggaagtaa 60 taataactat ggtccaggaa gttggtagcg agtaattaaa cgactatggt ccagatcgaa 120 gtcatcgaac caatcccagt tcccagacgg ccagagaata tgcagctctg aaaaagcaaa 180 aacacgtttc gacttgtcar tggtccataa tatataccct cctgtcattt tgacgtagtt 240 taattcccag tatatataaa tccacacaca acggaatcat cctacaccac cagaaaccct 300 cacaaccaac caagagtcca agacttgatc tctgccatgg ccaaacctcc accggcgagg 360 gccaaggctc gctcggttct cgccggcggc ctgatgaacc 400 <210> 69 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 69 atatttttca tctctttttc attgtcttgt acaaataatg aaaacctact acttaaattt 60 ccgagtttag gcatttcggc ggacaatgat aattgctatc tgtgcattgt gcatatcaag 120 gaaaagaatg gtcttcttca agaaattcac aatgaatgag tctgatattg aggaattcat 180 aaggattaat ggatcacttr cctcaaagcg gtacagttat ttaaaagtga aaaagatgac 240 aaacacattc aaagatcaga tagggaaagg aggatatggc actgtataca aaggaatcct 300 acctgatggc cttgttgtgg cagtgaaaat gctaagcgga tcaaaaggta acggagagga 360 ctttataaat gaagttgcta gcattggtag aacttctcat 400 <210> 70 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 70 taacgcaaca atgtctagtg ctgatttggg aattgccgac tgcaccagta ttccaaaaat 60 aagctaagca aagagaaacc atcaactaca acagaaaaag taaaacctaa agcactgcaa 120 cttgacaaaa gttggacatc ctcaatcgtg gtgtacactg agtacactga ggtacttttc 180 tatattaaat tatgttgcay ataaaataac ttatattcag tgttcataaa aaatacaagt 240 gcactcctta atgtccttca atttttactt gtattaacca aatgctagtc tgatcaagat 300 aaatgaatag agaggagact tctttttgtt tcttgggcat aaataaatac acagcatctt 360 tggtttctca acttatagga ctgacagaaa catgttacct 400 <210> 71 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 71 ttgggcagtc gaaattcacg ttcatagggt gacagatcct gagttttgga tccataaagg 60 gtcgatgagt ataacttcat caacataggc tattttttgg atcctgaatg cgggagcggt 120 aaaaccacat cgatataggc tttgatgttt tttggggcat tgggatctca gcttagattg 180 gggtctggtg cttttggtgr tggtgtggca ttagatgttt tgttccttgt cactaggcga 240 cgacgacgta tgtattgtga cgatgaccca tgaatttcgt ttgcatttag gccagctaga 300 tcttaaattt agttaatgct tttattttct agtttaattc caataattcc tttttgttaa 360 ggtactctag gcaggaatct aaacatgctt tattgtgcac 400 <210> 72 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 72 gattatttgg tttagtgacg aaaatttttc cttaaaatta ggatatctta aaaattgatt 60 caacaatgac agactgaagt tgatgcataa ttaagaaaga aagaaaaaga aaaaaagagc 120 agcaagaaag aaaaatgttc atacttgttg ctgtagtcac agttcttaac aaccgaacta 180 caaagccttg aaacaaaacy atcccaacca caaacagaac ccaataggaa gaagagacag 240 actttactaa agaaatgtcg tctctttcaa taatctccag ggcatctaga caagaatttg 300 ggcctctaga taagaatttg ggcacctaaa tacagattta ctttgacaaa ttgctattct 360 caaatccatc attttgagat ttatatgtaa taacttgaac 400 <210> 73 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 73 ttcataatta agttcctaga acaatcaaca aggcggctca tgtggttgtt ggtgttgtag 60 cccttggtag tgggcgtagt atttggttaa gatttgcaat gatcccgtag ctacgttggt 120 ttctaagggt gtagagtgaa gaaatttttt ccactactga tcgttctcat gttatgtacg 180 taattttacc tcaagcatcy aaatctctca ggaccttgtg atcgattcta cgaaaacaac 240 agtccacaac agggaattgg tagtcttaaa agtaaagata gaatgtaaaa ggatagcatg 300 caagcaactt agcaacatga tcaacctgca tatgcttaat taaataatga aactgagaag 360 aaaaagaaaa aaaaagtttg ttttgtcact tagtctggct 400 <210> 74 <211> 326 <212> DNA <213> Fragaria ananassa Duch. <400> 74 attctgttta aggctttaag ccaaacataa gtagcatcag taatccttct ctttcaggca 60 ttttctcagt aaccaaagag gcacattatg aatgcatcaa aagctttaca atagcaacac 120 ttaackaaac gtaattgaag caatatgaaa accaaaatag atatccaaaa caaaactgat 180 gatcaatttc gaaatcagat tgaggtttga gctaacctgg tgatctaggc atcgtgattg 240 accaaaagag caggcaacgt tcactagagc caaaacaaga atatacaact atggaatcgc 300 agtagcttaa aatgcacaca cagtga 326 <210> 75 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 75 catgaatgaa agaaagtagt ttccatcctg ttcaagtaat gcagcacctc ctacttcttc 60 tgtatatcta aatgggtgaa tgaaaaacaa acagctccaa agggttctag agtaacagtg 120 tcgccagagt tactgatctt tagaaaaaca tacgagaagc aaagctacca ggttaacatg 180 gaatacatat gagatatttr cccattactc gattcaaaca ttcaaattct acactttggt 240 gtaaagcatt gcctagagcc caacaagcca gggactcaaa attttaactc aaaattgaga 300 gaagaaatgc tttacttcca agggactcta aacttataat ccgacatttc agatgcttct 360 ttttcgaaat cgctgtatgg gctgaggagg aggagggctg 400 <210> 76 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 76 tgacatttgc taataataat ggtttgctac taagtactaa caattaatac tagtagcaca 60 tctagttatt gttatgctaa tgtatgtaca attggaatgg gaagtttagg aaatattgta 120 caatatttgt tactgattgg tgcatataaa cagaatacaa gcgcatcaat atgtaggaca 180 actactcttt gttgttcggr aatctttggt tgaaaattcg gtttatcatt ggatgtgcaa 240 tccttatttg ctttcttaga gctttttttt taagtttctc atttttgtta acaaacaaat 300 gcactatcaa gaatcaagaa atagaagaga tatagaagaa gaaacaaaat gggccaatat 360 acccatttta gataaaggat attcagggag gatgagaaac 400 <210> 77 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 77 aactactaaa aattagactt cttcgctttg gcgtcttagg actttttctt cttcgttttt 60 cttcacaata ctcgaataag ataacaccat gtgttaaata ttaatttggc cgatgttttt 120 aagtcaatcc aatgctaatc ccacaccaaa ggtttaatga cgataatttc attaaatttg 180 tatctccata atgaatttty tctgaaagag tgtataaaag aaaatgtttc gtaatttttg 240 agtctcgtcg atttgaaaat cattttataa gagtgaaaag ttttgtgata tagttcataa 300 tttgagtaag ctactaatat gttttttcat atggcttatt ttaaagaaaa aaaaaagacg 360 acaaattata tgaaatgaat gcaaagaagt acatggagcg 400 <210> 78 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 78 aataataaga tcaacccata caaacatttg cttttacagt gccgtttatt ttccttattc 60 ttctaagttc taactatgta tatacgaaat aattttggat cataaaatta tataaactaa 120 attgtggaaa taaaaataaa tggagattga ggctcattga caacgttttt ctttgggatc 180 aagttctctc aacgcatggy accagcacct ctatctctct ccctcaaaac acacaaaatc 240 acatcagaaa atgttggaat atacataaga atttgattct taaacaccac aataaaacat 300 taataggact gaagctaggc tttagatgcc atgaataaag tatatattga ttaaattgca 360 taggaaaaat gcaattagaa atgtagctgc tatctgattt 400 <210> 79 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 79 actgtgagaa tactcaattt tataaaaact tagttttaac gtcctccgtc tttttgctcc 60 gtagaatacg gtcctattct agggtttcac aatatggttt cttccttctg tctgttgacg 120 ctttcatcgg acgagacttt gtggtggcca gatggtggtc gggcttctta ggtcggtggt 180 ggtggatcga gagagggagr ctatccaaag agtggactgt gaggttggtt gatggtttga 240 gatgtagttt ggtttctctc caacatggtt gtctagtggt tgaacatggg caatgactag 300 tctcttaaag gtcctgcttc agacgacgac ccgacgatcg tggtagagcg gctgacttgg 360 tttcagacat gaggccactt atttgggccg gttcttggat 400 <210> 80 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 80 gggtctattt acaatatact atatactaaa accagtacat aaaaatattg ttcataaaga 60 ctcgtaaata aacctttctg tccctatttt ctgtttaatc atgagatttc gtgcgtcgct 120 tatccatatg tagccgctct tttctttctc gctctctatt acccatttat ttacaattat 180 gccattgctc tgtacgttty tgtctttctt cccctgtgag gatcattttg caatcgtaaa 240 aatataagat gtcaaaagtc cccgcagcaa ggtgcaggca ttttaccttg tttgttacaa 300 cactcataac agattttatt ttttgttgtt gaataaaaca gatttgttct aaaaaaaaaa 360 aacttaattt gagtatgaag agtgaaaatc aaaattagaa 400 <210> 81 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 81 tgtgttgtca tgtttggctc cgattagggt ctcatttgtt tggctagcaa gggtcgcctg 60 aaagtggaaa cttaggggac gtttggtgca ctggatttga tttgattaga tatccaatcc 120 tgatctagct tgactagatt ggattgagga tgtctaaccc catatcaaat acaatgtttg 180 gtgaggtgca ctcatggcar attggaaaag cggaaattcg ttgttttgca ttttcttttt 240 ctattctgct tttttttttt tgtcctcacg tctgagatac aatttttaca ataattaatt 300 ttgacatgta atttaataaa ttaaaactaa aaatagcata aaaataatta aaatcagacg 360 cacagtaaat actagaacaa ttataataaa atatgtgatg 400 <210> 82 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 82 atatctaact agtgtatatt tttcatagat aaaaactcaa ctatttgtac cgtatatgcg 60 tatgatttaa agtgaaattt taatttcata acacaaataa tgtagaaaat agtacgtttt 120 tgtttgaaac ggaggatgaa caagtacatg atccatcatt ttacaaacgc tacacaataa 180 ttcatcttgc aaacgtactm gtcttctaca taatacatat gttgatagtg atcgatcttc 240 ccgttgcaag atcacgtatc ttcatattgg tcagttgctt tgtttcattt ggcaaagata 300 gcaattaacc aacaagccat acgaatgctt cctctaacta tgtttggatc ccaagaaatg 360 atgacgaaaa tataaaattg agcattccaa ttatcatata 400 <210> 83 <211> 317 <212> DNA <213> Fragaria ananassa Duch. <400> 83 catatctaca gcatctagaa attgcaaact acccacttga tccaaaggtg tagagataag 60 atgatgactg gaatagtaga gcttgggaaa tcaatgtcaa ctcgagaagt caaggcactc 120 cattcatact aagagttgca aacttaaggg gacaactaca attgccttta ttggtaacga 180 tttcatccac tcggcccctr accacagaag gcatgcttat gaatcaacca tatgtagcta 240 ttttaggcat taatactttg gaaaagtttt ttccgattga cggagatata attgccacaa 300 tatgtgatta catattg 317 <210> 84 <211> 354 <212> DNA <213> Fragaria ananassa Duch. <400> 84 tcctgtggtt tgttttccca ccacacttgc ctggttttcc actgacttgt ttcttaaact 60 gatggaattg cagctaccaa aatcccagca gtacccaaag caacatctgc acaaagcggt 120 atgtattttt tatgtatatt gccatggtaa aacracagtc ttctgtgatt atcattaccc 180 catgtagaag cagatcacta catttcagcg tagtcacgtc aaatattgaa tgtagtatcc 240 attgacatgt ttttactttt agttcatact gaagataatc atattaattg cattaggtag 300 tgataacatt tgtttgaact tgaccgagtt aatacttagg cacaaaatta caaa 354 <210> 85 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 85 tgtataatga aagaaaaatt agtcatgaaa tttcacatta gctaaacaag aaaacataaa 60 atttgaagtc atggatccat atgagaatga tctccaatta ccaagtataa cagaaatttc 120 ctccagaaca catactctgc aactagcatg atctctttat cagtggttag atactgttgt 180 tctagtgtga ctcgatgttk tccgcatgat ggcacaaggc aaaagctgac gagcaataat 240 ttgaaggttc gaaatccggc gctccttgag cctcgatttt taccgatagg gtctggttgc 300 cttctatgtc gtttgagacg gaaacttgaa agactgttag atgtgtttga ttccgatagc 360 tatcgtaaga gccatcccct atatcagcca ccaaccatat 400 <210> 86 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 86 attgcttaat gacacatgaa gactcggtcg taccgctaag gccaatcatc actttgaggt 60 agattgtaat ctcgataatt actctatgag tatgaattgt aatcttttga tattgatgca 120 aaatacttat ctgaaagatc acgatccata ctcatagcgt aatgatcgag attacaattt 180 agctcataaa gtaatgatar gaattgtatg agtcgttgtg tgtcgttaag caattttgag 240 ataatgtgga aaaaaatatg aaagctgtta gtcaaaaatg aatttgtaaa aagaaatatg 300 aaagttatta gtcaaaaata aatttcgaat atgtagctag catgatatgc tttaaacccg 360 cacttccata cattttattt catgatcact aatagtctaa 400 <210> 87 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 87 ctaggtttct gatgagcaat ctggagctca tctttggttg tttgaagcgg cctctccggt 60 ctaagtctcc gttggccgcc actacatgct gcttaggagt tcagtgcctc gcaaacttga 120 tcattgctgt cacaaaaacc ttcaacagca cctggtccga tgtttccgaa atctatagct 180 tcttactcac ctttgctacm atgagtggca cctaccaggt acattactct tttgcttcat 240 gttcgatacc gcttggtgtt ctagaattga atacttgatg ttcatgttga gtaaatggat 300 ttgatttgga aacaggtgac aaaagaagcg tgttcctgtc ttcatcgtat actggaatgt 360 tttcaaggca cgccgctgct tgatcctgca agcaaaaata 400 <210> 88 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 88 tatgaagcta atgcagcagt ttgaatttat tccacaccaa attcccaaat ctgatattta 60 catcacaacc agtctcctac tcgtctgtca aattgagatg cacctttagt tctctttctt 120 gtgtatcagt catctacctt gacaaaaata tggacatctt aattcctgac agtctatact 180 ctgcagatat aatcagaagy tcctatatcc tcttaaacac caccacatct tataccaaaa 240 caatacatgt attctagaag ttaacaaaca attgttctat gattatcata atagcgttca 300 gctaaacaca caagcgaaaa agtgggcaca ctaaacacga aagattcaaa cacaatgtat 360 tgggaacttg ggatacctat tcacacggcg ctccctagga 400 <210> 89 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 89 acaaacaaat ctgttctctc acagggtaag aggtggtgct gaacagctga ttattcatgg 60 ggaacgctag gagaaagtga gagctcacat caatcatttg tagcagctat gagctcaacg 120 tcagcggagg cagagaccaa attaaaagag ccatactaaa gcttatacgt tatggctttt 180 tcatgatgat atgacaaaak ttagatgaaa ccattgtcca gaatgagagc gacaacgtca 240 caataagaaa gttgtgagac tctgaccaat gtttaatata aagatcctaa aacaatttaa 300 acagcaagat ccatcctcac aatacaaatc ctactgaaat cgaaattcac aacttgaagg 360 ccctttctgc atcagttaat tcacaagtac gtagggagga 400 <210> 90 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 90 gagatctttt tgctgccttt attttttggg caattaaaaa aaatgtttca tcaaagacac 60 taaagtcacg tttctaatag taaagatatg ttgagaaatc ttttgtacat cagattacac 120 tttcagagat tattaggcct actgcccaca actcggtggc taaataatac ataacaacag 180 agcttacatt tttcctgggm ttttttgggt cgaaattatt gggccttttt tctacgtgtt 240 ccatatatag cgcagattgt agccattgga aatttagttt atgttgtgta acgagaattt 300 ttcatctgct ggaccatgca ctttagaaat gacatatgga cagttacaaa ttagtgtctg 360 aaccttgtgt tcttgttctt gttatagtga agatagagaa 400 <210> 91 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 91 aattgctatt acaaaagtta ttaatatttt gtcattaaaa gatatttttg gctagtactg 60 gtattacaac aaaatacatt taagagagaa acaaaatact ggtattattc caaaaaaaat 120 atcaattgga tgaagtactg gtattacaac aaattacatt taagagagaa acgaaatact 180 ggctctgcta gggttcgttk tagcaccttc aaacatgatc tccatggaga ttttggcttg 240 gaattgttgg ggtttaaaca acgaaatctc gtatgacagt gagaaagaga gagaggggtt 300 ccccgaaaca tcaatcagat gttgcgtttc taggttgcgc tagatttggc tgagcttatt 360 gatttgggct atttcggtgc cccttttacc tggaaaggag 400 <210> 92 <211> 341 <212> DNA <213> Fragaria ananassa Duch. <400> 92 gcttcaagag ttccaacatg ctcgctcaac tcatgtagca acacagcaac ttaccaaggc 60 aacatgctct aatggccatt ttgtagctgc ctttgccaat tcagttcctc ctcttaatta 120 gttcagccaa acaggtagag macttagcta tataagacag ggtttggact ttctccaaca 180 aatgaagcta actgaggctt tagtggaaac acacactttt ctgcagtccc agcatatcga 240 acactggggt tctgatctct ttagtttggt agtatcattg acgaccttaa atttgctcct 300 gcacaccttg ccagaagtct aagtttctta tgcgcctaga a 341 <210> 93 < 211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 93 gacgtgcttg ccttaatgtg taagtactct gtactgttgg tgattctttg gcaggaatat 60 tggatttgat tcctgcttta tgagtcaagg gcatagctgc acagggggtt gtataattgt 120 actacgccta tgaacaaata ttatgtaatg tatatacacg taaatgtttg caaactgaac 180 gaaaatatat atacactagm aatggcaccc gcgcgttgcg ggttttgagg aatccacatg 240 tttgagaacc aatctttaaa agacagaaaa tttagttgat atgaatcatt tgataactgc 300 atgcatatta gttgtctgaa tacacacaga tacagatgat gctgcagtgt ttaaaagctg 360 ttcaaggcta actcggttac aacagcttgg tggacaactt 400 <210> 94 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 94 tatgattgtc tctcacgcta gttatatatc tctcatgctc atggtactgc tttacgtcaa 60 aattgctagg tgaatattga tcgctttaca tagcctgtta ctgctttcat tattgttaat 120 tggtgaatgt cattagtttt ggcatataag ggttatctca ttctataaca cgtacaatat 180 ttaggtatac aatcaattay acgtacactc aatattttat tttaaaatac aaaataaatg 240 tactataact agaaacagtt tcagtcctta ttgttgtccg tgtactttta ctatgagcat 300 ctgacagctt ttttgattaa tagatcactg gacagtgatg agtgaaagat gcagatgcac 360 atcgatgaat cgaaaaagaa gacctgaatg tagatcacca 400 <210> 95 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 95 atttgaattt agggtgtgta gattttacta gtcttgtttt aattcatgga taattttaag 60 tcgtagccaa acacgaaggc atgctaactg atgctatctg ttgcaatatg tgccaagaga 120 cacttttcgg ggaagccctc agttggattt atgagttttc cgctaattcc atttagtatt 180 tccaacagct agtgaatcgm tttgtagaac gtttcatatt gtgtatcgat agggaaaata 240 cctacagttg ttcaaagtta agccgaccgg atgttaagga agggttgaaa attttcatga 300 actaatggca agcggctacc gcacgttagg atttttgaca ataacgtagc agaggaagcc 360 tttaaacaag ctttaattgg gaaaattcat gtacgcggta 400 <210> 96 <211> 400 <212> DNA <213> Fragaria ananassa Duch. <400> 96 aaggcataca aactacatat atattaagat gaagaaagct agcttcggtg tgaaaattag 60 ttttgataat ggcttaataa tggtcagcgt tggaatcgtt taggtgagat gttcatattc 120 ataataataa gatttctatt attagaacgt tgacgcgtaa tttggaaaca tttgaagaaa 180 agaaaaatat aacaagcaay atccgtatac aagctaacta tagggacagt taatccattc 240 gattttcctt tgtgattagg tgatcatcta tcaaacattc accaactgca aaatatcaaa 300 tctatcaatt tagaaggaac tgtttttggt ggcaaaacaa attatcctac gtaaaacaca 360atttaaactt aacacgaacc atcactttaa tcaagtactg 400
Claims (6)
서열번호 38 및 39의 5' 위치에서부터 200번째; 및
서열번호 10, 26, 47, 57, 60, 61, 62, 74, 83, 84 및 92의 3' 위치에서 200번째 위치한 단일염기다형성 (single nucleotide polymorphism, SNP) 전부를 마커로 포함하는 딸기 순도검정 및 품종 판별용 마커 세트로서
상기 딸기는 설향, 챈들러, 사치노카, 도요노카 및 베니훗베 중 어느 하나 이상인 마커 세트.
200th from both ends of SEQ ID NOs: 1 to 9, 11 to 25, 27 to 37, 40 to 46, 48 to 56, 58 to 59, 63 to 73, 75 to 82, 85 to 91 and 93 to 96;
200th from the 5' position of SEQ ID NOs: 38 and 39; and
Strawberry purity test including all of the single nucleotide polymorphism (SNP) at the 200th position at the 3' position of SEQ ID NOs: 10, 26, 47, 57, 60, 61, 62, 74, 83, 84 and 92 as a marker and as a marker set for breed identification.
The strawberry is a marker set of at least one of Seolhyang, Chandler, Sachinoka, Toyonoka, and Benihutbe.
상기 마커는 검출 가능한 물질로 표지되어 있는 마커 세트.
According to claim 1,
The marker is a set of markers labeled with a detectable substance.
A kit for strawberry purity test and variety identification, comprising the marker set of claim 1.
상기 증폭 산물로부터 상기 딸기의 순도 및 품종을 결정하는 단계를 포함하는 딸기 순도검정 및 품종 판별 방법.
Amplifying the nucleic acid using the genomic DNA derived from strawberry as a template and the marker set for strawberry purity test and variety discrimination according to claim 1; and
and determining the purity and variety of the strawberry from the amplification product.
상기 핵산을 증폭하는 단계에서 얻어진 증폭 산물을 DNA 칩, 겔 전기영동, 방사성 측정, 형광 측정 또는 인광 측정을 통해 검출하는 단계를 포함하는 딸기 순도검정 및 품종 판별 방법.
5. The method of claim 4,
and detecting the amplification product obtained in the step of amplifying the nucleic acid through a DNA chip, gel electrophoresis, radiometric measurement, fluorescence measurement, or phosphorescence measurement.
상기 증폭 산물로부터 상기 딸기의 순도 및 품종을 결정하는 단계는, 얻어진 증폭 산물을, 제1항에 따른 딸기 순도검정 및 품종 판별용 마커 세트에 의해 공지된 딸기 품종의 핵산을 증폭한 결과와 비교함으로써 수행되는 것인 딸기 순도검정 및 품종 판별 방법.5. The method of claim 4,
In the step of determining the purity and variety of the strawberry from the amplification product, the obtained amplification product is compared with the result of amplifying the nucleic acid of a known strawberry variety by the strawberry purity test and variety discrimination marker set according to claim 1, Strawberry purity test and cultivar identification method to be performed.
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