KR102429915B1 - A composition comprising water extract of oat hull for preventing or treating menopausal symptoms - Google Patents

Abstract

The present invention relates to: a food composition for preventing or alleviating menopausal symptoms, which comprises an oat husk water extract or a fraction thereof; a feed composition; a pharmaceutical composition; and a method for preventing or alleviating menopausal symptoms using the food composition.

Description

A composition comprising water extract of oat hull for preventing or treating menopausal symptoms

The present invention provides a food composition for preventing or improving menopausal symptoms comprising an oat hull water extract or a fraction thereof; feed composition; pharmaceutical compositions; And it relates to a method for preventing or improving menopausal symptoms using the food composition.

The period from the onset of menopause to about one year is called the menopause transition period, often called menopause, and the period is generally about 4-7 years on average. Menopause refers to disorders of glucose metabolism, lipid metabolism, and energy metabolism and hormonal disorders, resulting in hot flashes, sweating, and atrophy of the genitourinary system in postmenopausal women (vaginal dryness, vaginitis due to recurrent vaginal infections and urinary tract infections; Menopause symptoms such as cystitis, dysuria, urgency) and mental instability (disruption of concentration and short-term memory, anxiety and irritability, and decreased memory) may occur.

As a candidate material for the prevention, improvement and treatment of these female menopausal symptoms, research on natural herbs has been actively conducted, and a red ginseng complex composition having an effect on improving female menopausal disorders (Korea Patent Publication No. 10-2006-0061323), etc. has been developed. has been However, their efficacy is still uncertain (Cano et al., 2008; Somjen et al., 2008).

Under this background, the present inventors have completed the present invention by confirming that the oat hull water extract has an effect of improving menopausal symptoms similar to female hormones.

One object of the present invention is to provide a food composition for preventing or improving menopausal symptoms comprising an oat hull water extract or a fraction thereof.

Another object of the present invention is to provide a feed composition for preventing or improving menopausal symptoms comprising an oat hull water extract or a fraction thereof.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating menopausal symptoms comprising an oat hull water extract or a fraction thereof.

Another object of the present invention is to provide a method for preventing or improving menopausal symptoms comprising administering the food composition to a subject other than humans.

Hereinafter, the content of the present invention will be described in detail as follows. On the other hand, descriptions and embodiments of one aspect disclosed in the present invention may be applied to descriptions and embodiments of other aspects with respect to common matters. Also, all combinations of the various elements disclosed herein are within the scope of the present invention. In addition, it cannot be seen that the scope of the present invention is limited by the specific descriptions described below.

One aspect of the present invention provides a food composition for preventing or improving menopausal symptoms comprising an oat hull water extract or a fraction thereof.

The term "oat" in the present invention is a biennial plant of the Grape family, whose scientific name is Avena sativa . The oat hulls of the present invention may refer to by-products other than the grains produced after processing of oats, but may also include oat hulls to which some grains are attached due to technical limitations. Oats and oat hulls may be raw, dried, or processed products, and commercially available ones may be purchased and used, or those collected or grown in nature may be used in the manufacture of extracts, but is not limited thereto.

As used herein, the term "extract" refers to a liquid component obtained by immersing a target substance in various solvents and then extracting it for a certain period of time at room temperature or in a heated state, and a solid obtained by removing the solvent from the liquid component. it means. The extraction may be performed one or more times, but is not limited thereto. In addition, it may be comprehensively interpreted as including all of the filtrate, diluent, concentrate thereof, crude product thereof, and purified product of the resultant product. Accordingly, the oat hull extract provided in the present invention is an extract obtained by extracting oat hulls, a purified product obtained by enzymatic decomposition of the extract, or a mixture thereof, and extracts of all formulations that can be formed using the extract itself and the extract. can be interpreted as including

The method of extracting the oat husks of the present invention is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include hot water extraction, ultrasonic extraction, filtration, reflux extraction, and the like, and these may be performed alone or in combination of two or more methods.

In the present invention, the type of solvent used for the extraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water, alcohol, or a mixed solvent thereof, and these may be used alone or in combination of two or more. 4 alcohols can be used. Specifically, it may be to use distilled water, but is not limited thereto.

For the purposes of the present invention, the extract of the present invention may be prepared by extracting oat hulls with water as an oat hull water extract.

As used herein, the term "fraction" refers to a result obtained by performing fractionation in order to separate a specific component or a specific component group from a mixture including various components.

The fractionation method for obtaining the fraction in the present invention is not particularly limited, and may be performed according to a method commonly used in the art. Non-limiting examples of the fractionation method include a fractionation method performed by treating various solvents, an ultrafiltration fractionation method performed by passing an ultrafiltration membrane having a constant molecular weight cut-off value, and various chromatography (size, charge, hydrophobicity or chromatographic fractionation method for performing separation according to affinity), and combinations thereof. Specifically, there may be mentioned a method of obtaining a fraction from the extract by treating the extract obtained by extracting the oat hulls of the present invention with a predetermined solvent.

In the present invention, the type of the fractionation solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohols having 1 to 4 carbon atoms; hexane (Hexan), ethyl acetate (Ethyl acetate), dimethyl chloride (Dimethyl chloride), chloroform (Chloroform), a nonpolar solvent such as dichloromethane (Dichloromethane); or a mixed solvent thereof. These may be used alone or in combination of one or more, but are not limited thereto, and specifically, hexane, ethyl acetate, butanol, or water may be used alone or in combination of one or more, but limited thereto doesn't happen

In addition, the extract or fraction may be prepared and used in the form of a dry powder after extraction and/or fractionation, but is not limited thereto.

As used herein, the term "menopausal symptoms" refers to symptoms and symptoms that appear in women before and after menopause as the secretion of female hormones decreases due to ovarian aging. The menopausal symptoms may be mixed with 'menopausal disease', 'menopausal syndrome' or 'menopausal symptoms'.

In the menopause, female hormones change, and in particular, it may be characterized by a decrease in the level or activity of estrogen, a female hormone, and various menopausal symptoms may be experienced along with these hormone changes.

The menopausal symptoms are not limited as long as the symptoms and symptoms appear in women before and after menopause, and female hormonal disorders, flushing, sweating, nervousness, depression, dizziness, fatigue, joint pain, muscle pain, headache, light palpitations, a sense of security, and sweating during sleep , sleep disturbance, dry skin, vaginal dryness, vaginal atrophy, lower urethral atrophy, dyspareunia, vaginitis, cystitis, dysuria, urgency, difficulty concentrating, memory impairment, anxiety, irritability, memory loss, dry skin, arthralgia, heart disease, high blood pressure , cardiovascular abnormalities, weight gain, may be selected from the group consisting of obesity, but is not limited thereto.

Specifically, the menopausal symptoms may be due to female hormonal disorders, but is not limited thereto.

During menopause, the secretion of estrogen (estradiol, estrone) decreases due to the depletion of follicles in the ovaries. Accordingly, as markers for improving menopause and measuring estrogen activity, estrogen receptor-α (ERα) and estrogen receptor-β (ERβ), estrogen response gene ESR1 (Estrogen receptor 1) and pS2 is known.

Accordingly, in one embodiment of the present invention, it was confirmed that when the cells were treated with the oat hull water extract, ERα and ESR1 decreased, and the expression of ERβ and pS2 increased. This result confirmed the same trend as when the positive control group, estradiol (E2), was treated. 1 to 4).

As used herein, the term "prevention" may refer to any act of suppressing or delaying the onset of menopausal symptoms by administering a composition comprising an oat hull water extract or a fraction thereof of the present invention to an individual.

As used herein, the term "improvement" may refer to any action for improving or benefiting symptoms of menopause by administering a composition comprising an oat hull water extract or a fraction thereof of the present invention to an individual.

As used herein, the term "food" refers to meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages , vitamin complexes, health functional foods, and health foods, and includes all foods in a conventional sense.

Since the food composition of the present invention can be consumed on a daily basis, an improvement effect on high female menopausal symptoms can be expected, and thus it can be very usefully used for health promotion purposes.

The functional food (functional food) is the same term as food for special health use (FoSHU), and in addition to supplying nutrients, it is processed to efficiently exhibit bioregulatory functions and has high medical effects. means food. Here, 'function (sex)' refers to obtaining useful effects for health purposes, such as regulating nutrients or physiological effects on the structure and function of the human body. The food of the present invention can be prepared by a method commonly used in the art, and at the time of manufacture, it can be prepared by adding raw materials and components commonly added in the art. In addition, the formulation of the food may be prepared without limitation as long as it is a formulation recognized as a food. The composition for food of the present invention can be prepared in various forms, and unlike general drugs, it has the advantage that there are no side effects that may occur during long-term administration of the drug using food as a raw material, and because it is excellent in portability, the present invention The food of the invention can be ingested as an adjuvant for enhancing the improvement effect of female menopausal disease.

The health food means a food having an active health maintenance or promotion effect compared to general food, and the health supplement food means a food for the purpose of health supplementation. In some cases, the terms health functional food, health food, and dietary supplement are preferred.

Specifically, the health functional food is a food prepared by adding the compound of the present invention to food materials such as beverages, teas, spices, gum, and confectionery, or encapsulating, powdering, suspension, etc., and when ingested, It means to bring an effect, but unlike general drugs, it has the advantage that there are no side effects that may occur when taking the drug for a long period of time by using food as a raw material.

The food composition may further include a physiologically acceptable carrier, the type of carrier is not particularly limited and any carrier commonly used in the art may be used.

In addition, the food composition may include additional ingredients that are commonly used in food compositions to improve odor, taste, vision, and the like. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, pantothenic acid, and the like may be included. In addition, minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), chromium (Cr); and amino acids such as lysine, tryptophan, cysteine, and valine.

In addition, the food composition includes a preservative (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), a disinfectant (bleaching powder and high bleaching powder, sodium hypochlorite, etc.), an antioxidant (butylhydroxyanisole (BHA), butyl hydro Loxytoluene (BHT), etc.), coloring agents (tar pigments, etc.), coloring agents (sodium nitrite, sodium nitrite, etc.), bleach (sodium sulfite), seasonings (MSG sodium glutamate, etc.), sweeteners (dulcin, cyclamate, saccharin, etc.) , sodium, etc.), flavorings (vanillin, lactones, etc.), swelling agents (alum, D-potassium hydrogen tartrate, etc.), strengthening agents, emulsifiers, thickeners (flavors), film agents, gum base agents, foam inhibitors, solvents, improvers, etc. It may contain food additives. The additive may be selected according to the type of food and used in an appropriate amount.

As an example of the food composition of the present invention, it may be used as a health drink composition, and in this case, it may contain various flavoring agents or natural carbohydrates as an additional component like a conventional drink. The above-mentioned natural carbohydrates include monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; polysaccharides such as dextrin and cyclodextrin; It may be a sugar alcohol such as xylitol, sorbitol, or erythritol. Sweeteners include natural sweeteners such as taumatine, stevia extract; A synthetic sweetener such as saccharin or aspartame may be used. The ratio of the natural carbohydrate may be generally about 0.01 to 0.04 g, specifically about 0.02 to 0.03 g per 100 mL of the health beverage composition of the present invention.

In addition to the above, the health beverage composition includes various nutrients, vitamins, electrolytes, flavoring agents, colorants, pectic acid, pectic acid salts, alginic acid, alginic acid salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, It may contain alcohol, a carbonation agent, and the like. In addition, it may contain the pulp for the production of natural fruit juice, fruit juice beverage, or vegetable beverage. These components may be used independently or in combination. Although the ratio of these additives is not very important, it is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the health beverage composition of the present invention.

Another aspect of the present invention provides a feed composition for preventing or improving menopausal symptoms comprising an oat hull water extract or a fraction thereof.

The oat hull water extract, fraction, menopausal symptoms, prevention, improvement, etc. are the same as those described in other embodiments.

The feed composition may include a known carrier, stabilizer, or additive acceptable for feed in addition to the oat hull water extract or fractions thereof. For example, there are binders, emulsifiers, preservatives, etc. added to prevent quality deterioration, and amino acids, vitamins, enzymes, flavoring agents, non-protein nitrogen compounds, silicate agents, buffers added to feed to increase efficacy , extractants, and oligosaccharides. In addition, it may further include a feed mixture, and the like, but is not limited thereto. The feed composition may contain various nutrients such as vitamins, amino acids, minerals, antioxidants, and other additives as necessary, and may be in a suitable state such as powder, granule, pellet, suspension, etc. The feed composition of the present invention may be supplied alone or mixed with feed for a unit animal. The feed of the present invention is not particularly limited, and any feed such as powder feed, solid feed, dry feed, wet feed, moist pellet feed, dry pellet feed, EP (Extruder Pellet) feed, raw feed, etc. may be used.

Another aspect of the present invention provides a pharmaceutical composition for preventing or treating menopausal symptoms comprising an oat hull water extract or a fraction thereof.

The oat hull water extract, fraction, menopausal symptoms, and prevention are the same as described in other embodiments.

As used herein, the term "treatment" refers to any action that treats or improves menopausal symptoms by administering a composition comprising an oat hull water extract or a fraction thereof of the present invention to an individual.

The "pharmaceutical composition" of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent. Such pharmaceutically acceptable carriers, excipients, or diluents may be non-naturally occurring. Specifically, the composition is formulated in the form of powders, granules, tablets, liquids, capsules, suspensions, emulsions, syrups, aerosols, etc., external preparations, suppositories, and sterile injection solutions according to conventional methods, respectively. can be used In the present invention, carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate , calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil. In the case of formulation, it is prepared using commonly used diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations include at least one excipient in the extract and its fractions, for example, starch, calcium carbonate, It is prepared by mixing sucrose or lactose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to simple diluents such as water and liquid paraffin, which are commonly used for suspensions, solutions, emulsions, syrups, etc. have. Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like can be used.

Another aspect of the present invention provides a method for preventing or treating menopausal symptoms comprising administering a pharmaceutical composition comprising an oat hull water extract or a fraction thereof to individuals other than humans,

As used herein, the term "individual" may refer to any animal, including humans, that has or is likely to develop menopausal symptoms. The animal may be a mammal, such as a cow, a horse, a sheep, a pig, a goat, a camel, an antelope, a dog, a cat, and the like, in need of treatment for symptoms similar to those of a human, but is not limited thereto. The subject in need of the composition of the present invention may be a human or non-human subject, but is not limited thereto.

As used herein, the term "administration" means introducing the composition of the present invention to an individual by any suitable method, and the route of administration of the composition of the present invention is through various routes, either oral or parenteral, as long as it can reach the target tissue. may be administered. Specifically, it may be a method of oral administration, but is not limited thereto.

Specifically, the prevention or treatment method of the present invention may include the step of administering the composition in a pharmaceutically effective amount to an individual who has or is at risk of developing menopausal symptoms.

The active ingredient of the pharmaceutical composition of the present invention may vary depending on the age, sex, weight, pathological condition and severity of the subject to be administered, the route of administration, or the judgment of the prescriber. Determination of the amount to be applied based on these factors is within the level of those skilled in the art, and the frequency of administration of the composition of the present invention is not particularly limited thereto, but may be administered once a day or administered several times in divided doses. The above dosage does not limit the scope of the present invention in any way.

The oat hull water extract, fraction, pharmaceutical composition, individual, administration, menopausal symptoms, prevention, and treatment are the same as described in other embodiments.

The oat hull water extract or a fraction thereof of the present invention can improve menopausal symptoms like estradiol as a natural product. Accordingly, industrial use can be expected in the related food, drug, and feed industries.

1 is a diagram showing that the oat hull water extract reduces ERα.
Figure 2 is a diagram showing that the oat hull water extract reduces ESR1.
3 is a diagram showing that the oat hull water extract increases ERβ.
4 is a diagram showing that the oat hull water extract increases pS2.
5 is a diagram comparing the dietary amount of a normal control group, an ovariectomized control group, an ovariectomized and estradiol treated group, and an ovariectomized and oat hull water extract treated group.
6 is a diagram showing that the oat hull water extract increases the size of the uterus in menopausal mice.
7 is a diagram showing that the oat hull water extract reduces body temperature in menopausal mice.
8 is a diagram showing that the oat hull water extract reduces cardiovascular parameters in menopausal mice.

Hereinafter, the present invention will be described in more detail through examples. However, these Examples and Experimental Examples are for illustrative purposes of the present invention, and the scope of the present invention is not limited to these Examples and Experimental Examples.

Example 1: Preparation of oat hull water extract

After adding 10 L of distilled water to 1 kg of oat hulls, stirring at room temperature for 24 hours, extraction was performed, filtration with filter paper, and this process was repeated 3 times.

Example 2: Confirmation of the effect of improving menopausal symptoms of oat hull water extract ( in vitro )

Since menopausal improvement is directly related to hormones, estrogen receptor-α (ERα) and estrogen receptor-β (ERβ) and ESR1, an estrogen response gene, are markers for measuring menopausal improvement and estrogen activity. (Estrogen receptor 1) and pS2 are known.

To confirm estrogen activity, protein expression of ERα or ERβ or its mRNA level can be measured, and it can be evaluated whether a test substance exhibits estrogen activity by analyzing the change in the mRNA level of ESR1 or pS2 gene.

Therefore, in order to confirm the menopausal improvement effect of the oat hull water extract, the breast cancer cell line MCF-7 cell was treated with an oat hull water extract (experimental group) or estradiol (E2) (positive control group, 100㎍/㎖) , mRNA levels of ERα, ERβ, ESR1, and pS2 genes were analyzed by RT-PCR.

As a result, it was confirmed that when MCF-7 cells were treated with the oat hull water extract, ERα and ESR1 decreased, and the expression of ERβ and pS2 increased. This result confirmed the same trend as when the positive control estradiol (estradiol; E2) was treated (FIGS. 1 to 4).

Example 3: Confirmation of the effect of improving menopausal symptoms of oat hull water extract ( in vivo )

3-1: Menopause animal model production

A menopausal animal model was produced by ovariectomy for ICR mice (4 weeks old, female) who had undergone a one-week adaptation period. Normal control group (sham), ovariectomy control group (OVX), ovariectomy + estradiol 0.5 mg/kg treatment group (E2), ovariectomy + oat hull water extract 400 mg/kg treatment group (OBW100), and It was divided into ovariectomized + oat hull water extract 400mg/kg treatment group (OBW400). Their food intake was all at a similar level (FIG. 5), and after all of them were bred for 7 weeks, their body temperature was measured and sacrificed to confirm the change in menopausal symptoms.

3-2: Confirmation of increase in uterus size due to administration of oat hull water extract

Menopause is highly related to female hormones, and hormones have a significant effect on reproductive organs, so the size of the uterus was measured by administering oat hull water extract to an animal model of menopause.

To this end, the size of the uterus (uterus) removed from the sacrificed mice of Example 3-1 was compared. As a result, compared to the normal control group, when the ovaries were resected, the size of the uterus was significantly reduced, whereas the ovariectomized + oat hull water extract 400mg/kg treatment group (OBW100), the ovariectomized + oat hull water extract 400mg/kg treatment group (OB400) It was confirmed that the size of the uterus was significantly increased as in the ovariectomy + estradiol 0.5 mg/kg (E2) treatment group (FIG. 6).

3-3: Confirmation of decrease in body temperature due to administration of oat hull water extract

Since the body temperature increases in individuals with menopause symptoms, the temperature change was measured by administering an oat hull water extract to a menopause animal model.

To this end, as a result of measuring the tail temperature of the mice of Example 3-1, the body temperature increased when the ovaries were resected compared to the normal control group, whereas the ovariectomized + oat hull water extract 400 mg/kg treated group (OBW100), ovariectomized In the + oat hull water extract 400 mg/kg treatment group (OB400), it was confirmed that the body temperature dropped as in the ovariectomy + estradiol 0.5 mg/kg (E2) treatment group (FIG. 7).

3-4: Confirmation of improvement of cardiovascular indicators by administration of oat hull water extract

Estrogen relaxes blood vessels and reduces blood lipids. As a result, cardiovascular indicators (cholesterol and TG, etc.) increase in individuals with menopause symptoms. was measured.

To this end, blood from the mice of Example 3-1 was collected, serum was extracted, and cardiovascular indicators were measured (Asanset total cholesterol kit, AM202-K, Asan Pharmaceutical; Asanset triglyceride, AM157S-K, Asan Pharmaceutical; and Mouse Estradiol (E2) ELISA Kit). As a result, the concentration of triglyceride (TG) and total cholesterol increased when the ovaries were resected compared to the normal control group, while the ovariectomized + oat hull water extract 400mg/kg treatment group (OBW100), ovariectomized In the + oat hull water extract 400 mg/kg treatment group (OB400), it was confirmed that the cardiovascular index level was reduced as in the ovariectomy + estradiol 0.5 mg/kg (E2) treatment group (FIG. 8).

The above results suggest that the oat hull water extract functions like female hormones and has the effect of improving menopause.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims described below and their equivalents.

Claims (5)

A food composition for improving female hormonal disorders, comprising an oat hull water extract or a fraction thereof, wherein the improvement of female hormonal disorders is estrogen activity enhancement or flushing improvement, the food composition.
delete As a feed composition for improving female hormonal disorders, comprising an oat hull water extract or a fraction thereof,
The improvement of the female hormonal disorder is to improve estrogen activity or improve flushing,
The feed composition is for mammals, the feed composition.
delete A method for enhancing estrogen activity or improving flushing comprising administering the food composition of claim 1 to a mammal other than a human.

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