KR102428987B1 - Composition Containing Extracts of Camellia sinensis - Google Patents

Abstract

The present invention provides a composition containing an extract of a tea tree, preferably an extract of a new tea tree variety (Jangwon No. 3), and more particularly, epigallocatechin-3- O-(3-O - methyl) gallate (epigallocatechin- 3-O-(3-O-methyl) gallate; EGCG3”Me; 3” -O -Me-EGCG) in a high content, and relates to a composition containing an extract of Camellia sinensis as an active ingredient. The composition comprising, as an active ingredient, an extract of tea tree containing epigallocatechin-3- O-(3-O - methyl) gallate in a high content according to the present invention has antioxidant effects due to free radical scavenging and inhibition and cola It has an anti-inflammatory effect by inhibiting the expression and secretion of inflammatory factors such as prostaglandin and IL-8 in skin cells, and has an anti-cancer effect on cancer cell lines derived from various tissues. great.

Description

Composition Containing Extracts of Camellia sinensis

The present invention provides a composition containing an extract of a tea tree, preferably an extract of a new tea tree variety (Jangwon No. 3), and more particularly, epigallocatechin-3- O-(3-O - methyl) gallate (epigallocatechin- 3-O-(3-O-methyl) gallate; EGCG3”Me; 3” -O -Me-EGCG) in a high content, and relates to a composition containing an extract of Camellia sinensis as an active ingredient.

As human skin ages, it undergoes changes due to various internal and external factors. In other words, internally, the secretion of various hormones that control metabolism is reduced, the function and activity of immune cells is lowered, so the biosynthesis of immune proteins and biological constituent proteins necessary for the living body is reduced, and externally, due to the destruction of the ozone layer Due to the increase in the content of ultraviolet rays reaching the earth's surface in the sun's rays and the increase in environmental pollution, free radicals and active free radicals increase, thereby reducing the thickness of the skin, increasing wrinkles, and decreasing elasticity. The complexion of the skin also becomes dull, skin troubles occur frequently, and various changes such as freckles, freckles and age spots increase.

As aging progresses, symptoms of a change or decrease in the content and arrangement of collagen, elastin, hyaluronic acid, and glycoprotein, which are substances constituting the skin, appear, and oxidative stress caused by free radicals and free radicals is applied. In addition, cyclooxygenase-2 (Cox-2, cyclooxygenase), which is an enzyme that produces proinflammatory cytokines known to cause inflammation, in most cells constituting the skin due to aging or UV rays ) increases, the biosynthesis of matrix metalloproteinase (MMP), an enzyme that decomposes skin tissue by these inflammatory factors, increases, and NO (nitric oxide) production by iNOS (inducible nitric oxide synthase) is known to increase.

That is, the biosynthesis of matrix materials is reduced by the decrease in cell activity and micro-inflammation according to the naturally occurring endogenous aging, and external factors such as the increase in stress caused by various harmful environments and the increase in reactive oxygen species by sunlight. As the decomposition and degeneration are accelerated, the skin matrix is destroyed and thinned, and various symptoms of skin aging appear. Therefore, it is a reality that many studies are being conducted on active ingredients that can prevent and improve these aging phenomena.

Research on antioxidants started with the discovery of SOD (superoxide dismutase), an enzyme that scavenges superoxide radicals by McCord and Fridovich in 1969. As I became interested in it, it started in earnest.

Free radicals such as superoxide are produced in the body by external stress, ultraviolet rays, smoking, drugs, and free radicals, and have non-selective and irreversible destructive action on cellular components such as lipids, proteins, sugars, and nucleic acids (eg, DNA). It is known to cause various diseases such as aging, cancer, brain diseases such as stroke and Parkinson's disease, heart disease, ischemia, arteriosclerosis, skin diseases, digestive diseases, inflammation, rheumatism, and autoimmune diseases. In this way, free radicals cause various functional disorders due to oxidative destruction of cells, thereby causing aging and diseases.

Recently, as it has been revealed that active oxygen and superoxide act as direct causes of various diseases and aging, antioxidant research is shifting from research on the development of antioxidants as food additives to research on antioxidants as anti-aging and disease treatment agents. .

The tea tree is an evergreen tree belonging to the family Camellia (Family: Theaceae ) and the genus Camellia (Genus: Camellia ), and the scientific name is Camellia sinensis . Cultivated tea trees can be divided into Assam species ( var. assamica ) and Chinese species ( var. sinesis ), and the Assam species has large leaves, is distributed in tropical and subtropical regions such as India and Sri Lanka, and has weak cold resistance, but black tea It is widely used as a dragon, and if the Chinese species has leaflet characteristics, it is distributed in temperate regions such as Korea, China, and Japan and has strong cold resistance.

The Korean native tea tree is mainly classified as a Chinese species. The tea tree is a subtropical crop, and the optimum temperature for growth is 14~16℃ in average annual temperature. Low temperature is a limiting factor for tea tree growth, and even in varieties with strong cold resistance, leaves die when 1 hour passes at -15℃, and high temperature failure occurs when it exceeds 40℃. The minimum amount of precipitation required for the growth of tea trees is 1,300 mm, but for optimal tea cultivation, more than 1,500 mm of precipitation is required per year.

In the case of Korea, it is reported that Daeryeom imported tea seeds from China in 828 at the end of the Silla Dynasty and planted it around Mt. Its native habitat is mainly in the southern region south of the cultivation limit line with an average temperature of 13℃, and is cultivated in Jeonbuk, Jeonnam, Gyeongnam, and Jeju.

Tea is characterized by containing a lot of nitrogen compounds, polyphenols, sugars, organic acids, vitamins and minerals compared to other favorite beverages. It is also known that the components of these amino acids are correlated with the price of the product. The total nitrogen content of tea leaves has traditionally been an important factor in determining the quality of tea along with amino acids.

About 20% by weight of the total nitrogen content of tea leaves is caffeine, and other nitrogen compounds include amino acids, amides, proteins, and nucleic acids. Tea protein is hardly eluted because it binds to tannins during the manufacturing process or is coagulated by heating.

In addition, tea has been widely used since ancient times because it has various pharmacological actions as well as a favorite beverage. In recent years, research has been actively conducted on the functionality of tea. Tea contains a lot of polyphenols in particular, and most of the polyphenols in tea are flavonoids known as catechin compounds.

Theanine is a component with a high specific gravity accounting for about 40 to 60% by weight of the total free amino acids in green tea. Theanine component has a great influence on the umami taste of tea, and is correlated with tea quality along with total free amino acids and total nitrogen, and is used as an important index in quality evaluation along with sensory evaluation, which is an important selection for the development of new tea tree varieties. is evaluated as a factor.

On the other hand, the tea tree is an omnivorous crop in which cross-pollination occurs between individuals with different genotypes, and there is severe inter-individual variation. Therefore, conventional tea trees that are generally cultivated are not single varieties, but hybrids that have been passed down through sexual reproduction for a long time. Each tea tree has different genetic traits, so harvest time, growth rate, leaf shape and color, composition, disease resistance, cold resistance There are several different characteristics such as Although these native species have high genetic value for breeding, it is urgent to develop tea tree varieties that can maintain uniform quality while exhibiting excellent functionality even after multiple breeding.

For example, a breeding method (Korean Patent Publication No. 10-2011-0020676) of a new tea tree variety (named "Jangwon No. 1") that has a higher catechin content than that of a conventional tea tree and has excellent antioxidant effect (Korean Patent Application Laid-Open No. 10-2011-0020676), a conventional tea tree A method of breeding a new tea tree variety (named "Jangwon No. 2") with a higher amino acid and theanine content compared to that (Korean Patent Application Laid-Open No. 10-2012-0107751) has been developed. All of these breeding methods are characterized in that excellent genetic traits can be repeatedly reproduced without mutations by selecting tea trees with target characteristics from among native tea trees and propagating them asexually by cuttings.

As such, although the method of breeding new tea tree varieties containing a large amount of specific components is somewhat known, tea tree varieties are difficult to improve because they are omnivorous and genetically very complex in addition to the problem that tea trees take a long breeding period like other perennial plants. , the efficacy of the tea leaf extract obtained from these tea trees has not been specifically revealed.

Accordingly, the present inventors analyzed the ingredients and efficacy of extracts obtained from tea leaves of a new tea tree variety (Jangwon No. 3) produced through a breeding method and cultivation method of a new tea tree variety, and applied it to pharmaceuticals, cosmetics, food, etc. Results of diligent efforts , an extract of a tea tree, preferably an extract of a new tea tree variety (Jangwon No. 3), is particularly epigallocatechin-3-O-(3- O -methyl) gallate (epigallocatechin-3-O-(3- O -methyl) ) gallate; EGCG3”Me; 3” -O -Me-EGCG) in a high content, and found that it has excellent antioxidant, anti-inflammatory, anti-aging and anti-cancer effects, and completed the present invention.

An object of the present invention is epigallocatechin-3- O- (3- O -methyl) gallate; EGCG3”Me; 3” according to the present invention -O -Me-EGCG) to provide a composition containing an extract of a tea tree containing a high content, preferably an extract of a new tea tree variety (Jangwon No. 3) as an active ingredient.

Another object of the present invention is to obtain an extract of a tea tree containing epigallocatechin-3- O-(3-O - methyl) gallate in a high content, preferably a new tea tree variety (Jangwon No. 3) according to the present invention. It contains the extract as an active ingredient, and provides a pharmaceutical composition, a cosmetic composition or a health functional food composition having antioxidant, anti-inflammatory, anti-aging and anti-cancer uses.

In order to achieve the above object,

The present invention relates to a composition comprising, as an active ingredient, an extract of a tea tree containing epigallocatechin-3- O-(3-O - methyl) gallate in a high content, preferably an extract of a new tea tree variety (Jangwon No. 3) as an active ingredient. provides

The present invention also contains an extract of a tea tree containing epigallocatechin-3- O-(3-O - methyl) gallate in a high content, preferably an extract of a new tea tree variety (Jangwon No. 3) as an active ingredient. It provides an antioxidant pharmaceutical composition, cosmetic composition or health functional food composition.

The present invention also contains an extract of a tea tree containing epigallocatechin-3- O-(3-O - methyl) gallate in a high content, preferably an extract of a new tea tree variety (Jangwon No. 3) as an active ingredient. It provides an anti-inflammatory pharmaceutical composition, cosmetic composition or health functional food composition.

The present invention also contains an extract of a tea tree containing epigallocatechin-3- O-(3-O - methyl) gallate in a high content, preferably an extract of a new tea tree variety (Jangwon No. 3) as an active ingredient. It provides an anti-aging pharmaceutical composition, cosmetic composition or health functional food composition.

The present invention also contains an extract of a tea tree containing epigallocatechin-3- O-(3-O - methyl) gallate in a high content, preferably an extract of a new tea tree variety (Jangwon No. 3) as an active ingredient. It provides an anticancer pharmaceutical composition, a cosmetic composition or a health functional food composition.

The composition comprising, as an active ingredient, an extract of tea tree containing epigallocatechin-3- O-(3-O - methyl) gallate in a high content according to the present invention has antioxidant effects due to free radical scavenging and inhibition and cola It has an anti-inflammatory effect by inhibiting the expression and secretion of inflammatory factors such as prostaglandin and IL-8 in skin cells, and has an anti-cancer effect on cancer cell lines derived from various tissues. great.

1 shows an elite line selection of collected green tea tree germplasm.
Figure 2 shows the propagation method (single cutting propagation) by a single cutting of selected green tea trees.
3 shows a new tea tree variety (Jangwon No. 3).
Figure 4 shows the superoxide anion scavenging activity of a new variety of tea tree (Jangwon No. 3) extract.
Figure 5 shows the hydroxyl radical scavenging activity of a new variety of tea tree (Jangwon No. 3) extract.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is those well known and commonly used in the art.

Hereinafter, the present invention will be described in more detail.

In an embodiment of the present invention, the selected new tea tree variety (New Cultivar of Camellia sinensis), a new tea tree variety (Jangwon No. 3) (seed accession number: KCTC (Korean Collection for Type Cultures) 12213BP (2012.5.14)); No.] after preparing a tea leaf extract), and then analyzing the components of the extract, the total content of catechin is 41.1% by weight based on the total weight of the extract in the extract of tea tree new variety (Jangwon No. 3) tea leaves, general It was found to be higher than that of Yabugida leaf extract (28.1% by weight), Kanayamidori leaf extract (26.7% by weight), and Yutagamidori leaf extract (26.4% by weight), which are tea tree-introduced varieties. In addition, the extract of a new tea tree variety (Jangwon No. 3) is epigallocatechin-3- O- (3- O -methyl) gallate; EGCG3”Me; 3” -O -Me-EGCG) was contained in an amount of 2.5% by weight based on the total weight of the extract, and it was confirmed to contain 10 times higher content than the general tea tree introduced variety (Example 1 and Test Example 1, Table 6).

On the other hand, as a result of comparative analysis of the catechin and caffeine components of the extract of the leaf of the new tea tree variety (Jangwon No. 3) and the extract of the leaf of Yabugida produced as a tea product, the extract of the leaf of the new tea tree variety (Jangwon No. 3) was found to be Yabu The total content of catechins was higher than that of Giga extract. In addition, the content of 3” -O -Me-EGCG) in the leaf extract of a new tea tree variety (Jangwon No. 3) was 4.7mg g ^-1 , 3.6 in green tea, oolong tea (semi-fermented tea) and black tea (fermented tea), respectively. mg·g ^-1 , 1.94 mg ^· g -1 (see Example 2 and Test Example ¹ , Table 7).

In another embodiment of the present invention, the antioxidant effect of the extract of a new tea tree variety (Jangwon No. 3) according to the present invention was confirmed. As a result, as shown in Table 8, (i) in the DPPH (1,1-diphenyl-2-picrylhydrazyl; 1,1-diphenyl-2-picryl hydrazyl) test, a new variety of tea tree (Jangwon No. 3) The extract (Example 1) showed a similar antioxidant effect compared to ascorbic acid (vitamin C), an antioxidant used as a positive control, and extracts of Yabugida, Kanayamidori and Yutagamidori of the general tea tree introduced in Comparative Examples 1 to 3 showed a significantly higher antioxidant effect compared to that, (ii) in the superoxide anion scavenging activity test, the extract of a new variety of tea tree (Jangwon No. 3) exhibited higher superoxide anion scavenging activity than the positive control, ascorbic acid, (iii) ) In the hydroxyl radical scavenging activity test, the extract of a new variety of tea tree (Jangwon No. 3) showed higher hydroxyl radical scavenging activity than the Yabugida extract (see Test Example 2).

In another embodiment of the present invention, the anti-aging effect of the extract of a new tea tree variety (Jangwon No. 3) according to the present invention was confirmed through its ability to inhibit collagenase production. As a result, as shown in Table 9, the lower the expression level of collagenase, the higher the ability to inhibit the expression of collagenase. . That is, the extract (Example 1) of a new tea tree variety (Jangwon No. 3) according to the present invention effectively inhibited the expression of collagenase in vitro , and the ability to inhibit the expression of collagenase rather than tocopherol used as a positive control. This excellence was confirmed. In particular, the extract (Example 1) of a new tea tree variety (Jangwon No. 3) according to the present invention showed more collagenase expression than the Yabugida, Kanayamidori and Yutagamidori extracts of the general tea tree introduced varieties of Comparative Examples 1 to 3 By effectively inhibiting collagen degradation in the skin, it was confirmed that the skin elasticity enhancement effect and the wrinkle reduction effect were excellent (see Test Example 3).

In another embodiment of the present invention, the anti-inflammatory effect of the extract of a new tea tree variety (Jangwon No. 3) according to the present invention was confirmed through the inhibitory effect on the production of prostaglandins and the inhibitory effect on the production of interleukin-8 (IL-8). did. As a result, as shown in Table 10, it was found that the extract (Example 1) of a new tea tree variety (Jangwon No. 3) according to the present invention had a very high prostaglandin production inhibitory effect like the control treated with aspirin, It was found that the inhibitory effect on prostaglandin production was significantly higher when compared with the extracts of Yabugida, Kanayamidori, or Yutagamidori of the general tea tree introduced in Examples 1 to 3. In addition, as shown in Table 11, it was found that the extract (Example 1) of a new tea tree variety (Jangwon No. 3) according to the present invention had a very high effect of inhibiting the secretion of IL-8 increased by PGSA, an inflammation-inducing stimulus. , It was found that the secretion of IL-8 was significantly reduced and inhibited compared to the Yabugida, Kanayamidori or Yutagamidori extracts of the general tea tree introduced varieties of Comparative Examples 1 to 3 (see Test Example 4).

In another embodiment of the present invention, the anticancer effect of the extract of a new tea tree variety (Jangwon No. 3) according to the present invention was confirmed through the cancer cell proliferation inhibitory effect. As a result, as shown in Table 12, when (i) each cancer cell line was treated with the extract of a new tea tree variety (Jangwon No. 3) at a concentration of 100 μg·mL ^-1 , the cancer cell proliferation rate of 50% or less in all cancer cell lines (survival rate); When the extract of a new tea tree variety (Jangwon No. 3) was treated at a concentration of 200 μg mL ^-1 to each cancer cell line, 12 in lung cancer cell line (A549), prostate cancer cell line (LNCaP) and breast cancer cell line (MCF-7), respectively. %, 24% and 16% of cancer cell proliferation rates, it was confirmed that the extract of a new variety of tea tree (Jangwon No. 3) has an inhibitory effect on cancer cell proliferation. (ii) In addition, as a result of treating each cancer cell line with 3" -O -Me-EGCG isolated from the extract of a new variety of tea tree (Jangwon No. 3), it inhibited the proliferation of cancer cells in a concentration-dependent manner for all cancer cell lines, especially the prostate. When a cancer cell line (LNCaP) was treated with 3" -O -Me-EGCG at concentrations of 50 μg mL ^-1 , 100 μg mL ^-1 and 200 μg mL ^-1 , the cancer cell proliferation rate was 46% and 37.7, respectively. % and 20%, which showed a higher cancer cell proliferation inhibitory effect than the extract of a new tea tree variety (Jangwon No. 3), and the breast cancer cell line (MCF-7) also showed high cancer cell proliferation when treated with 3" -O -Me-EGCG under the same conditions. It showed an inhibitory effect, and when 3" -O -Me-EGCG was treated with a concentration of 50 μg·mL ^-1 , 100 μg·mL ^-1 and 200 μg·mL ^-1 in lung cancer cell line (A549), cancer cell proliferation rate These were 41.0%, 20.0%, and 16.0%, respectively, and the lung cancer cell line (A549) exhibited higher cancer cell proliferation inhibitory efficacy than the prostate cancer cell line (LNCaP) and the breast cancer cell line (MCF-7) (see Test Example 5).

In another embodiment of the present invention, sensory evaluation of teas containing an extract of a new tea tree variety (Jangwon No. 3) according to the present invention was performed. As a result, as shown in Table 13, regardless of the processing method in the sensory evaluation, the new tea tree variety (Jangwon No. 3) had higher palatability in all of semi-fermented tea, fermented tea, and post-fermented tea than Yabugida and Okumidori. was evaluated (see Test Example 6).

Accordingly, in one aspect, the present invention provides epigallocatechin-3- O- (3- O -methyl) gallate; EGCG3”Me; 3”- O -Me-EGCG) containing a high content of the tea tree ( Camellia sinensis ) It relates to a composition containing an extract as an active ingredient.

The extract of the tea tree may be characterized as an extract of a new tea tree variety (Jangwon No. 3), but is not limited thereto.

The composition may be an antioxidant, anti-inflammatory, anti-aging and anti-cancer pharmaceutical composition, a cosmetic composition, or a health functional food composition, but is not limited thereto.

The content of epigallocatechin-3- O-(3-O - methyl) gallate contained in the extract of the tea tree is 1.25 wt% or more, preferably 1.25-8.00 wt%, based on the total weight of the extract, more Preferably it is 1.88-7.00 weight%, Most preferably, it is 2.25-6.00 weight%.

In another aspect, the present invention provides epigallocatechin-3- O- (3- O -methyl) gallate; EGCG3 ”Me; 3”-O- Me-EGCG) as an antioxidant pharmaceutical composition, cosmetic composition or health functional food composition containing an extract of the tea tree ( Camellia sinensis ) containing a high content as an active ingredient,

The content of epigallocatechin-3- O-(3-O - methyl) gallate contained in the extract of the tea tree is 1.25 wt% or more, preferably 1.25-8.00 wt%, based on the total weight of the extract, more Preferably from 1.88 to 7.00% by weight, most preferably from 2.25 to 6.00% by weight.

In another aspect, the present invention provides epigallocatechin-3- O- (3- O -methyl) gallate; EGCG3”Me; 3” -O -Me-EGCG) as an anti-inflammatory pharmaceutical composition, cosmetic composition, or health functional food composition containing an extract of the tea tree ( Camellia sinensis ) containing a high content as an active ingredient,

The content of epigallocatechin-3- O-(3-O - methyl) gallate contained in the extract of the tea tree is 1.25 wt% or more, preferably 1.25-8.00 wt%, based on the total weight of the extract, more Preferably from 1.88 to 7.00% by weight, most preferably from 2.25 to 6.00% by weight.

In another aspect, the present invention provides epigallocatechin-3- O- (3- O -methyl) gallate; EGCG3”Me; 3” -O -Me-EGCG) as an anti-aging pharmaceutical composition, cosmetic composition or health functional food composition containing an extract of the tea tree ( Camellia sinensis ) containing a high content as an active ingredient,

The content of epigallocatechin-3- O-(3-O - methyl) gallate contained in the extract of the tea tree is 1.25 wt% or more, preferably 1.25-8.00 wt%, based on the total weight of the extract, more Preferably from 1.88 to 7.00% by weight, most preferably from 2.25 to 6.00% by weight.

In another aspect, the present invention provides epigallocatechin-3- O- (3- O -methyl) gallate; EGCG3”Me; 3” -O -Me-EGCG) as an anticancer pharmaceutical composition, cosmetic composition or health functional food composition containing an extract of the tea tree ( Camellia sinensis ) containing a high content as an active ingredient,

The content of epigallocatechin-3- O-(3-O - methyl) gallate contained in the extract of the tea tree is 1.25 wt% or more, preferably 1.25-8.00 wt%, based on the total weight of the extract, more Preferably from 1.88 to 7.00% by weight, most preferably from 2.25 to 6.00% by weight.

A new tea tree variety (Jangwon No. 3) according to the present invention (seed accession number: KCTC (Korean Collection for Type Cultures) 12213BP (2012.5.14)) was supplied from Jangwon Co., Ltd. The new tea tree variety (Jangwon No. 3) has excellent antioxidant, anti-aging, skin whitening, anti-cancer, and anti-allergic effects, and was obtained for the purpose of developing a variety of functional tea tree containing a large amount of catechin, a functional ingredient that is effective in preventing and improving various stress-related diseases. As a cultivar, a small number of tea trees with high catechin content were selected based on the evaluation of growth characteristics and component content analysis after performing a separation breeding method on the genetic resources of native tea trees in Andeok-myeon, Seogwipo-si, Jeju-do, collected in 2006. Since the agronomic characteristics of the selected tea tree resources were not good, crossbreeding with excellent breeding lines was carried out in consideration of quality and quantity. The seeds were sown in the breeding house for crossbreeding, and after planting in the main field, secondary selection was carried out through component analysis and plant characteristic evaluation in 2010. Agronomic properties were measured according to the International Union for the Protection of New Varieties of Plants (UPOV) of the National Seed Resources of Korea (see Preparation Examples below).

In the present invention, the extract of the tea tree may be at least one type of extract selected from the group consisting of flowers, leaves, fruits, stems and roots of the tea tree, but is not limited thereto, and preferably an extract of the tea tree leaves. to be.

In particular, the tea tree according to the present invention has a high catechin content in its leaves. Epigallocatechin-3- O - among the catechins contained in the extract of the tea tree, preferably the extract of the new tea tree variety (Jangwon No. 3). The content of (3- O -methyl) gallate is, based on the total weight of the extract, 1.25 wt% or more, preferably 1.25-8.00 wt%, more preferably 1.88-7.00 wt%, most preferably 2.25-6.00 wt% % by weight.

The extract of the tea tree is epigallo contained in the flowers, leaves, fruits, stems or roots of the tea tree according to the harvest time (harvest season), or the leaf harvesting time (leaf season) of the flowers, leaves, fruits, stems or roots of the tea tree. The content of catechin-3- O-(3-O - methyl) gallate may be different. For example, in the case of a new tea tree variety (Jangwon No. 3), when the leafing period is May, 2.50% by weight, 4.16% by weight in August, or 5.36% by weight in November, epigallocatechin-3- O-(3-O - methyl) ) a tea tree leaf extract containing gallate based on the total weight of the extract can be obtained.

In the present invention, the epigallocatechin-3- O-(3-O -methyl) gallate is EGCG having an O - methylated form . , 3” -O -Me-EGCG or EGCG3”Me.

The tea tree according to the present invention, in particular, has a high catechin content in its leaves. The extract of the tea tree, preferably the extract of a new tea tree variety (Jangwon No. 3), contains gallocatechin (GC), epigallocatechin ( epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), epicatechin gallate, ECG) and a total of 8 types of catechins of catechin gallate (CG) based on the total weight of the extract, 20.55-82.2% by weight, preferably 30.8-61.65% by weight, more preferably 37-51.38% by weight contains with

In the present invention, the extract of the tea tree, preferably the extract of the new tea tree variety (Jangwon No. 3) may be characterized in that it contains 0.01 to 20% by weight based on the total weight of the composition. When the extract of the tea tree, preferably the extract of the new tea tree variety (Jangwon No. 3) is contained in an amount of less than 0.01% by weight, antioxidant effect, anti-inflammatory effect, anti-aging effect, and anti-cancer effect cannot be expected, and extract of tea tree, preferably When the content of the extract of a new tea tree variety (Jangwon No. 3) exceeds 20% by weight, skin irritation due to cytotoxicity may be concerned.

A new tea tree variety (Jangwon No. 3) according to the present invention comprises the steps of selecting a tea tree having the following characteristics (1) to (5) from a conventional tea tree; collecting the selected tea tree when the lower part of the stem from which the first tea has not been harvested begins to harden; And it is characterized in that it is obtained by the breeding method of a new tea tree variety comprising a; and the step of cutting the first section with one leaf and the second section with two leaves, and obtaining a new tea tree variety that is propagated by asexual propagation by cuttings.

The selected tea tree is characterized by having the following characteristics (1) to (5):

(1 ) Epigallocatechin-3- O-(3-O - methyl) gallate among catechins contained in tea tree extract (eg, tea leaf extract ) ; EGCG3”Me; 3” -O -Me-EGCG) content is, based on the total weight of the extract, 1.25% by weight or more, preferably 1.25-8.00% by weight, more preferably 1.88-7.00% by weight, most preferably usually 2.25-6.00 wt%;

(2) The blade width is 37-47 mm, preferably 39-46 mm, more preferably 43-45 mm, most preferably 44.1 mm, and the blade length is 85-95 mm, preferably 87-95 mm, more preferably 94 ~95mm, most preferably 94.2mm, the leaf area is 2200-3200mm ² or 4000-4500mm ^{2 , preferably 2500-2800mm 2 or 4100-4300mm 2 , more} preferably 4150-4200mm ² , most preferably 4154.2 mm ² ;

(3) Leaf shape is oblong, hair shape is medium, leaf color is green;

(4) New bud length is 145.0-165.0mm, preferably 150.0-165.0mm, more preferably 162.0-164.0mm, most preferably 163.5mm, and white bud length is 123.0-143.0g/100bud, preferably 128.0- 139.0 g/100 bud, more preferably 136.0 to 139.0 g/100 bud, most preferably 138.3 g/100 bud; and

(5) the male of the eggplant is upright; characterized in that, it provides a breeding method.

In the present invention, the selected tea tree may be a tea tree additionally having the following characteristics (6) to (17).

(6) chlorophyll content (soil & plant analyzer development, SPAD) 58~62;

(7) the total amino acid (TFAA) content is 3.50% by weight or more;

(8) the theanine content is 2.20% by weight or more;

(9) the total nitrogen content is not less than 6.00% by weight;

(10) the caffeine content is 23.5 mg/g or less;

(11) The number of branches is medium, the thickness and density of the branches are medium;

(12) germination period is late March;

(13) the flowering start time is September, the full bloom start time is October, the full bloom end time is October, and the flowering end time is November;

(14) May, June-August and October 3-4 harvests available;

(15) Jo Man-seong is rebirth, cold resistance is strong, and disease resistance is medium;

(16) The extract of tea tree, based on the total weight of the extract, 6.35-25.4% by weight, preferably 9.53-19.05% by weight, more preferably 11.4-15.9% by weight epigallocatechin (EGC), 9.4- 37.6% by weight, preferably 14.1 to 28.2% by weight, more preferably 16.9 to 23.5% by weight epigallocatechin gallate (EGCG) and 2.65 to 10.6% by weight, preferably 3.98 to 7.95% by weight, more preferably containing 4.77-6.63% by weight of epicatechin gallate (ECG); and

(17) Gallocatechin (GC), epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin gallate contained in tea tree extract , EGCG), gallocatechin gallate (GCG), epicatechin gallate (ECG) and catechin gallate (CG), the total content of catechins is 20.55-82.2 based on the total weight of the extract. % by weight, preferably 30.8 to 61.65% by weight, more preferably 37 to 51.38% by weight.

In the present invention, the breeding method may further include the step of immersing in water for 1 to 2 hours by obliquely cutting the bottom of the cut branch leaving about 2-3 cm from the lowest leaf after planting. .

In the present invention, general cultivation conditions of native tea trees are applied, and for example, it can be carried out as follows. Immediately after cutting, shade the sun so that the ray transmittance is around 30-45%. As cuttings are absorbed from the lower mortar like cut flowers and survive until rooted, watering is usually sufficient in the morning and evening. About 1 month after cutting, the root is rooted, and the cuttings are slightly excised. After rooting, 50 to 60% of the maximum water capacity is appropriate. Fertilization should be done from August and it may be started from additional fertilization (September). Weeding and pest control on seedlings should be carried out with care, and the sun shade should be removed after sufficient rooting and growth.

In addition, epigallocatechin-3- O -(3- O -methyl ) gallate contained in tea tree; EGCG3”Me; 3”-O - Me - In order to increase the content of EGCG), a tea tree was grown using a method of growing a new variety of tea tree ( Camellia sinensis ), characterized in that the tea tree is grown under the following conditions (1) to (5):

(1) line transmittance of 30-45%;

(2) watering twice a day for rooting of cuttings;

(3) Watering excision at rooting 1 month after cutting;

(4) The maximum water capacity after rooting is 50~60%; and

(5) Fertilization starts in August or September.

The cultivated tea tree is not particularly limited, but it is preferably a new tea tree variety according to the present invention having the following characteristics (1) to (16):

(1) Epigallocatechin-3- O- (3- O -methyl) gallate (epigallocatechin-3- O- (3- O -methyl) among catechins contained in the extract of a new tea tree variety (eg, tea leaf extract) gallate; EGCG3”Me; 3” -O -Me-EGCG) is, based on the total weight of the extract, 1.25% by weight or more, preferably 1.25-8.00% by weight, more preferably 1.88-7.00% by weight, most preferably 2.25 to 6.00% by weight;

(2) chlorophyll content (soil & plant analyzer development, SPAD) 58~62;

(3) the total amino acid (TFAA) content is 3.50% by weight or more;

(4) the theanine content is 2.20% by weight or more;

(5) the total nitrogen content is not less than 6.00% by weight;

(6) Caffeine content not more than 23.5 mg/g;

(7) The blade width is 37-47 mm, preferably 39-46 mm, more preferably 43-45 mm, most preferably 44.1 mm, and the blade length is 85-95 mm, preferably 87-95 mm, more preferably 94 ~95mm, most preferably 94.2mm, the leaf area is 2200-3200mm ² or 4000-4500mm ^{2 , preferably 2500-2800mm 2 or 4100-4300mm 2 , more} preferably 4150-4200mm ² , most preferably 4154.2 mm ² ;

(8) Leaf shape is oblong, hair shape is medium, leaf color is green;

(9) New bud length is 145.0 to 165.0 mm, preferably 150.0 to 165.0 mm, more preferably 162.0 to 164.0 mm, most preferably 163.5 mm, and white bud weight is 123.0 to 143.0 g/100 bud, preferably 128.0 to 139.0 g/100 bud, more preferably 136.0 to 139.0 g/100 bud, most preferably 138.3 g/100 bud;

(10) The number of branches is erect, the number of branches is medium, and the thickness and density of branches are medium;

(11) germination period is late March;

(12) the flowering start time is September, the full bloom start time is October, the full bloom end time is October, and the flowering end time is November;

(13) May, June-August and October 3-4 harvests available;

(14) Cho Man-seong is rebirth, cold resistance is strong, disease resistance is middle;

(15) The extract of the new tea tree variety is 6.35 to 25.4% by weight, preferably 9.53 to 19.05% by weight, more preferably 11.4 to 15.9% by weight epigallocatechin (EGC), 9.4 based on the total weight of the extract ~37.6% by weight, preferably 14.1 to 28.2% by weight, more preferably 16.9 to 23.5% by weight epigallocatechin gallate (EGCG) and 2.65 to 10.6% by weight, preferably 3.98 to 7.95% by weight , more preferably containing 4.77 to 6.63% by weight of epicatechin gallate (ECG); and

(16) Gallocatechin (GC), epigallocatechin (EGC), catechin (C), epicatechin (EC), epigallocatechin contained in the extract of new tea tree varieties The total content of catechins of gallate, EGCG), gallocatechin gallate (GCG), epicatechin gallate (ECG) and catechin gallate (CG) is 20.55~ 82.2% by weight, preferably 30.8 to 61.65% by weight, more preferably 37 to 51.38% by weight.

In the present invention, a pharmaceutical composition containing an extract of a tea tree, preferably an extract of a new tea tree variety (Jangwon No. 3), is a pharmaceutical composition such as a preservative, a stabilizer, a wetting agent or an emulsification accelerator, a salt and/or a buffer for regulating osmotic pressure. It may further contain adjuvants and other therapeutically useful substances (carriers, excipients, diluents, etc.), and may be formulated in various oral or parenteral administration forms according to conventional methods.

The term “carrier” is defined as a compound that facilitates the addition of the compound into a cell or tissue. For example, dimethylsulfoxide (DMSO) is a commonly used carrier that facilitates the introduction of many organic compounds into cells or tissues of living organisms.

The term "excipient" is a substance added to make the drug easier to eat or to give it a certain color and shape when the amount of the main drug is small in the preparation process of tablets or pills, and lactose or starch is mainly used do.

The term “diluent” is defined as a compound that not only stabilizes the biologically active form of the compound of interest, but is diluted in water which will dissolve the compound. Salts dissolved in buffer solutions are used as diluents in the art. A commonly used buffer solution is phosphate buffered saline because it mimics the salt state of human solutions. Because buffer salts can control the pH of a solution at low concentrations, buffer diluents rarely modify the biological activity of a compound.

A composition containing an extract of a tea tree, preferably an extract of a new tea plant variety (Jangwon No. 3), may be administered to a human patient as such or in combination with other active ingredients as in combination therapy or in admixture with suitable carriers or excipients. As a composition, it can be administered.

Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

In addition, the oral administration agents include powders, granules, tablets, capsules, syrups, etc., and the parenteral administration agents include, for example, injections, drops, ointments, lotions, gels, creams, sprays, suspensions, It may be in the form of an emulsion, suppository (坐劑), patch, etc., but is not limited thereto.

The pharmaceutical composition according to an embodiment of the present invention may be administered parenterally, rectally, topically, transdermally, or the like. The pharmaceutical composition according to an embodiment of the present invention may be administered topically to the scalp, for example.

In addition, the pharmaceutically acceptable dose of the active ingredient, that is, the dosage may be determined according to the age, sex, and weight of the subject to be treated, the specific disease or pathological condition to be treated, the severity of the disease or pathological condition, the route of administration, and the judgment of the prescriber. It will be different. Dosage determination based on these factors is within the level of one of ordinary skill in the art. The dosage is, for example, 1 to several times to be administered in an amount of 0.01 to 5000 mg, preferably 0.1 to 2000 mg, more preferably 0.5 to 500 mg, and most preferably 1 to 100 mg per 1 kg of body weight per day. It can be administered in divided doses, but the dosage does not limit the scope of the present invention in any way.

Pharmaceutical compositions suitable for use in the present invention include compositions in which the active ingredients, including extracts of tea tree, preferably extracts of a new variety of tea tree (Jangwon No. 3), are contained in an amount effective to achieve its intended purpose. do. More specifically, a therapeutically effective amount means an amount of a compound effective to prolong the survival of the subject to be treated, or to prevent, alleviate or ameliorate symptoms of a disease. Determination of a therapeutically effective amount is within the ability of one of ordinary skill in the art, particularly in light of the detailed disclosure provided herein.

The therapeutically effective amount for the extract of the tea tree used in the methods of the present invention, preferably the extract of a new variety of tea tree (Jangwon No. 3), and the composition (compounds) containing it as an active ingredient is initially measured from cell culture analysis can be For example, a dose can be calculated in an animal model to obtain a circulating concentration range that includes an IC ₅₀ (half maximal inhibitory concentration) or EC ₅₀ (half maximal effective concentration) determined in cell culture. Such information can be used to more accurately determine useful doses in humans.

The toxicity and therapeutic efficacy of the extract of the tea tree described here, preferably the extract of a new tea tree variety (Jangwon No. 3), or the composition (compounds) containing it as an active ingredient, is, for example, LD ₅₀ (of the population In order to determine the lethal dose to 50%), ED ₅₀ (dose having a therapeutic effect on 50% of the population), IC ₅₀ (dose having a therapeutically inhibitory effect on 50% of the population), in cell culture or experimental animals It can be estimated by the standard constraint processes of . The dose ratio between toxicity and therapeutic effect is the therapeutic index and it can be expressed as the ratio between LD ₅₀ and ED ₅₀ (or IC ₅₀ ). Compounds exhibiting high therapeutic indices are preferred. Data obtained from these cell culture assays can be used to estimate a range of doses for use in humans. The dosage or application of such compounds is preferably within a range of circulating concentrations that include the ED ₅₀ (or IC ₅₀ ) with little or no toxicity.

In the present invention, the formulation of the pharmaceutical composition is preferably any one selected from the group consisting of ointments, lotions, gels, creams, sprays, suspensions, emulsions, and patches. When the composition according to the present invention is applied to a pharmaceutical composition, the composition may be formulated in a solid, semi-solid or liquid form by adding a commercially available inorganic or organic carrier to the composition as an active ingredient. The active ingredient of the present invention can be easily formulated according to a conventional method, and a surfactant, excipient, colorant, spice, stabilizer, preservative, preservative, wetting agent, emulsification accelerator, suspending agent, salt for osmotic pressure control and/or Buffers and other commercially available adjuvants may be appropriately used.

In the present invention, the pharmaceutical composition may be a pharmaceutical composition effective in treating cancer or tumor.

The term “cancer” or “tumor” refers to or describes a physiological condition in mammals that is typically characterized by unregulated cell growth/proliferation. Examples of cancer include, but are not limited to, carcinoma, lymphoma (eg, Hodgkin's and non-Hodgkin's lymphoma), blastoma, sarcoma, and leukemia. More specific examples of such cancers include squamous cell cancer, small cell lung cancer, non-small cell lung cancer, adenocarcinoma of the lung, squamous cell carcinoma of the lung, peritoneal cancer, hepatocellular carcinoma, gastrointestinal cancer, pancreatic cancer, glioma, cervical cancer, ovarian cancer, liver cancer, Bladder cancer, hepatocellular carcinoma, breast cancer, colon cancer, colorectal cancer, endometrial or uterine carcinoma, salivary gland carcinoma, kidney cancer, liver cancer, prostate cancer, vulvar cancer, thyroid cancer, liver carcinoma, leukemia and other lymphoproliferative disorders, and various types of two including cervical cancer. In the present invention, the cancer is preferably prostate cancer, lung cancer or breast cancer.

In the present invention, the cosmetic composition containing the extract of the tea tree, preferably the extract of the new tea tree variety (Jangwon No. 3) is a composition for external application to the skin, and is a softening lotion, astringent lotion, nutritive lotion, lotion, eye cream, nourishing cream, massage It may be characterized in that it is formulated with any one selected from the group consisting of cream, cleansing cream, cleansing foam, cleansing water, powder, essence, and pack.

In addition, the composition according to the present invention is a fatty substance, an organic solvent, a solubilizer, a thickening agent, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, a surfactant, water, an ionic or non ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic actives, lipid vesicles or any other ingredients commonly used in cosmetics; It may contain adjuvants commonly used in the field of cosmetics or dermatology. The adjuvant is introduced in an amount generally used in the field of cosmetology or dermatology. In addition, the composition of the present invention may contain a skin absorption promoting substance in order to increase the skin improvement effect.

In addition, useful carriers for the cosmetic composition include, for example, water, acetone, ethanol, ethylene glycol, propylene glycol, butane-1,3-diol, isopropyl myristate, isopropyl palmitate or mineral oil. The carrier may be a solution, colloidal dispersant, emulsion (oil-in-water or water-in-oil), suspension, cream, lotion, gel, foam, mousse, spray, and the like. Other ingredients that may be selected according to the intended use of the carrier and/or combination may further be included. Additional possible ingredients include water-soluble colorants (such as FD&C Blue #1); oil-soluble colorants (such as D&C Green #6); water-soluble sunscreens (such as Eusolex 232); oil-soluble sunscreens (such as octyl methoxycinnamate); particulate sunscreen (such as zinc oxide); antioxidants (such as BHT); chelating agents (such as disodium EDTA); emulsion stabilizers (such as carbomers); preservatives (such as methyl paraben); fragrances (such as pinene); flavorings (such as sorbitol); humectants (such as glycerin); waterproofing agents (such as PVP/eicocene copolymers); water-soluble film-forming agents (such as hydroxypropyl methylcellulose); oil-soluble film-forming agents (such as hydrogenated C-9 resins); cationic acid polymers (such as polyquaternium 10); Anionic polymers (such as xanthan gum), vitamins (such as tocopherol), and the like may be included, but are not limited thereto.

In the present invention, 'functional food' or 'functional food' means a food in which the functionality of a general food is improved by adding an extract of a tea tree according to the present invention, preferably an extract of a new tea tree variety (Jangwon No. 3) to general food. do.

Functionality can be roughly divided into physical properties and physiological functionality. When the extract of the present invention is added to general food, the physical properties and physiological functionality of general food will be improved, and the present invention comprehensively describes the improved functional food as 'functional food'. (Health functional food)' or 'Functional food (Health functional food)'.

In the present invention, the term 'health functional food' refers to a food manufactured and processed using raw materials or ingredients useful for the human body according to Act No. 10219 of the Health Functional Food Act, and provides nutrients for the structure and function of the human body. It refers to ingestion for the purpose of obtaining useful effects for health purposes, such as controlling or physiological effects.

A health functional food containing an extract of a tea tree according to the present invention, preferably an extract of a new tea tree variety (Jangwon No. 3), has a synergistic effect on the main effect within the range that does not impair the main effect aimed at the present invention. It may contain other ingredients and the like. For example, in order to improve physical properties, additives such as fragrances, pigments, bactericides, antioxidants, preservatives, humectants, thickeners, inorganic salts, emulsifiers, and synthetic polymers may be further included. In addition, auxiliary components such as water-soluble vitamins, oil-soluble vitamins, high molecular peptides, high molecular weight polysaccharides and seaweed extract may be further included. The components can be appropriately selected and formulated by those skilled in the art without difficulty depending on the formulation or purpose of use, and the amount added may be selected within a range that does not impair the purpose and effect of the present invention. In addition, the composition of the present invention itself can be used in the form of an additive to other foods.

The formulation of the health functional food containing the extract of the tea tree according to the present invention, preferably the extract of the new tea tree variety (Jangwon No. 3) is not particularly limited, but, for example, tablets, granules, drinks, beverages, solutions, emulsions, It can be formulated in various forms such as a viscous mixture, tablet, powder, liquid, and tea, and is preferably a functional tea composition. In addition, when administering the health functional food, it may be administered by various methods such as simple drinking, injection administration, spray method, or squeeze method.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

[ production example ] Breeding method for improving tea tree varieties

The tea tree disclosed in the present specification ( Camellia sinensis (L). ) Jangwon No. 3, a new variety, evaluated the chemical composition of the tea tree (see Table 2 below) and growth characteristics by performing a hybrid breeding method on genetic resources of native and wild tea trees. For tea tree specimens selected by evaluation through analysis such as test (see Table 3 below), branches that have passed the first growing season (early May) are used as cuttings and nurtured through asexual propagation process, which is the same as the parameters of the tea tree. As a new variety with excellent properties (see Table 4 below), it was developed at the Sulloc Tea Research Institute located at 1241 Seogwangseo-ri, Andeok-myeon, Seogwipo-si, Jeju-do, Korea.

The new variety of tea tree finally selected based on the analysis result was named "Jangwon No. 3", and the new variety, Jangwon No. 3, completed the seed deposit with the Korea Research Institute of Bioscience and Biotechnology (seed accession number: KCTC (Korean Collection for Type Cultures)). 12213BP (2012.5.14)).

In this embodiment, the cropping method and cultivation management were performed according to the standard farming rotation of the Rural Development Administration of Korea.

Briefly describing the hybrid breeding method, the hybrid breeding is primarily performed by selecting genetic resources containing a high content of 3" -O -Me-EGCG, a specific functional component, from among domestic native tea tree genetic resources, and then After crossing the circle with the parent model, the progeny were obtained and then nurtured, but an elite line containing a high content of 3" -O -Me-EGCG was nurtured.

Among the elite lines, tea trees having excellent basic growth characteristics were finally cultivared based on the following characteristics. Each of the following characteristics is preferably measured based on the International Plant Genetic Resource Evaluation Standard (UPOV) of the National Seed Resources of the Republic of Korea.

(i) germination period and leaf characteristics (leaf shape, hair growth, leaf color, leaf width, leaf length, leaf area, chlorophyll content, etc.);

(ii) Neonatal characteristics: Shin-A-Jang and Baek-A-Jung;

(iii) Branch characteristics: number, number, thickness, and density of branches;

(iv) flowering characteristics: flowering start time, full bloom start time, full bloom end time and flowering end time; and

(v) other characteristics: early ripeness, cold resistance, disease resistance and harvest season

The growth characteristic test is, more specifically, during the first water cycle (April 24 to May 5), at the time of opening 70% for newborns, a grid of 20 cm in width and length is high on a tea tree and 1 in the grid All shoots up to the three core parts were collected, and the average values of growth indices such as premature man- age, leaf length, leaf width, new height, and new height were measured according to the International Plant Genetic Resource Evaluation Standard of the National Seed Resources of the Republic of Korea. Manseong Jo was compared by examining the sprouting period when the proportion of germinated sprouts among all sprouts was 70%. The length of the leaf stem from the base of the bud to the uppermost leaf was measured. The new weight was evaluated as the weight of 100 sprouts. The leaf length, leaf width, and leaf area were measured on the third leaf from the upper part of the collected newborn baby, and the leaf area was measured using a leaf area meter (LI-3100 Area Meter, LI-COR. Inc., Japan).

Table 1 below shows the agronomic characteristics, which are the selection criteria of new tea tree varieties.

characteristic Selection Criteria germination period germination period Late March (March 19 to March 29) lobe characteristics leaf type long oval Mo Yong (毛茸) middle leaf color green Blade width (mm) 37-47 Card length (mm) 85-95 Leaf area (mm ² ) 2200~3200 Chlorophyll content (SPAD) 55~75 neonatal characteristics New height (mm) 145.0~165.0 Baek Ah Jung (g/100 bud) 123.0~143.0 branch characteristics sousa upright wash middle branch thickness middle branch density middle flowering characteristics start to bloom From September 17th to September 27th start to bloom September 30th - October 10th end of full bloom October 14th to October 24th End of flowering From November 15th to November 25th Jo Man-seong rebirth cold resistance river disease resistance middle harvest 3 times a year (preferably in May, July, October)

Table 2 below shows the chemical composition characteristics for the evaluation of chemical components, which are the selection criteria for new tea tree varieties.

Ingredient Content Characteristics content range 3” -O -Me-EGCG (mg/g) 4.00 and above Total amino acid (TFAA) content (% by weight) 3.50 or higher Theanine content (wt%) 2.20 or later Nitrogen content (wt%) 6.00 and above Caffeine content (mg/g) 23.5 or less Total catechin content (mg/g) 100.0 or less

The "SPAD" value described herein is a value measured by a non-destructive method that predicts the chlorophyll content or nitrogen content of a leaf by measuring the green degree of the leaf using a chlorophyll meter (SPAD-502, Minolta, Japan) means "SPAD(Soil & Plant analyzer development)" is a measuring instrument developed by the Soil & Plant analyzer development (SPAD) of the Japanese Ministry of Agriculture, Forestry and Fisheries. The principle of this instrument is to use light emitting diodes to generate light in the wavelength band of 650nm, which is sensitive to chlorophyll, and 940nm, which is insensitive to chlorophyll, and pass this light through a 2*3mm window, and then have a thickness of 1.2mm. The intensity of light passing through the leaves of a plant within is measured with a silicon photodiode, and the chlorophyll content is expressed as a value between -9.9 and 99.9 using the difference between the two wavelength values.

In addition, using Near-Infrared Reflectance Spectroscopy (NIRs) and High Performance Liquid Chromatography (HPLC), theanine, total amino acids (TFAA), total nitrogen, caffeine, catechins Component analysis was performed. Samples for analysis are collected 100g from the first to third leaves of the upper shoot based on the time when the opening degree of the first five leaves is 70% during the first water car (early May) period, and passed through the steamer (TERADA) for 40 seconds After steaming and drying in a dryer at 80°C, the powder was processed into powder with a pulverizer and sieved through a 60 mesh sieve.

For near-infrared spectroscopy, NIR Analyzer (NIRs-XDS, Foss) was used to measure the near-infrared absorption spectrum (400-2500 nm) for each sample and calculate the individual content.

Table 3 below shows the growth characteristics, which are the selection criteria for new varieties of tea tree.

characteristic Selection Criteria germination period germination period March 21 - March 27 lobe characteristics leaf type long oval Mo Yong (毛茸) middle leaf color green Leaf width (mm) 39-44 Card length (mm) 87-92 Leaf area (mm ² ) 2500-2800 Chlorophyll content (SPAD) 58-62 neonatal characteristics New height (mm) 150.0~160.0 Baek Ah Jung (g/100 bud) 128.0~138.0 branch characteristics sousa upright wash middle branch thickness middle branch density middle flowering characteristics start to bloom September 20th - September 24th start to bloom October 3 to October 7 end of full bloom October 17th - October 21st End of flowering From November 18th to November 22nd Jo Man-seong rebirth cold resistance river disease resistance middle harvest May, July, October

"Jangwon No. 3", a new tea tree variety obtained by hybrid breeding through the above selection criteria, has the characteristics shown in Table 4 below.

characteristic Manor 3 results germination period germination period March 22 lobe characteristics leaf type long oval Mo Yong (毛茸) middle leaf color green Leaf width (mm) 44.1 Card length (mm) 94.2 Leaf area (mm ² ) 4154.2 Chlorophyll content (SPAD) 63.1 neonatal characteristics New height (mm) 163.5 Baek Ah Jung (g/100 bud) 138.3 branch characteristics sousa upright wash river branch thickness middle branch density middle flowering characteristics start to bloom September 20 start to bloom October 3 end of full bloom October 20 End of flowering November 26 Jo Man-seong early childhood cold resistance river disease resistance middle harvest May, June, August, October

[ Example 1] Preparation of extracts of new tea tree varieties

Jangwon No. 3, a new tea tree cultivar selected by the above-mentioned breeding method, was collected from the upper 1st to 3rd leaves of the shoots based on the time when the opening rate of the first 5 leaves was 70% during the first water tea (early May) period. It was steamed by passing it through hot air for 40 seconds, dried in a dryer at 80° C., and then powdered with a pulverizer, sieved through a 60 mesh sieve, and powdered. 1 L of 70% (v/v) aqueous ethanol solution (absolute ethanol 70% (v/v) + distilled water 30% (v/v)) was added to 100 g of the thus-prepared new tea tree variety (Jangwon No. 3) pulverized sample and refluxed. After extraction and filtration, it was concentrated under reduced pressure at 40-45° C. to finally obtain 19.2 g of a dried extract (Example 1) of a new tea tree variety (Jangwon No. 3) leaves.

[ comparative example One] Yabugida (general tea tree variety) Preparation of extracts

In Example 1, except that Yabugida, a common tea tree introduced variety, was used instead of a new tea tree variety (Jangwon No. 3), it was prepared in the same manner as in Example 1, and finally 17.5 g of a dried extract of Yabugida leaves was obtained.

[ comparative example 2] Kanaya Midori (general tea tree variety) Preparation of extracts

In Example 1, except that Kanayamidori, a general tea tree introduced variety, was used instead of a new tea tree variety (Jangwon No. 3), it was prepared in the same manner as in Example 1, and finally 17.1 g of a dried extract of Kanayamidori leaves was obtained.

[ comparative example 3] Yutagami-dori (general tea tree variety) Preparation of extracts

In Example 1, a dried extract of Yutagami-dori leaves was obtained in the same manner as in Example 1, except that Yutagami-dori, a common tea tree introduced variety, was used instead of the new tea tree variety (Jangwon No. 3). did.

[Example 2] Preparation of extracts of new varieties of tea tree

For tea Preparation of extracts

After the tea leaves of Jangwon No. 3, a new tea tree variety, were collected by cultivation period, various tea products were manufactured according to the conventional processing method. Briefly, green tea (unfermented tea) that prevents the fermentation process by inactivating oxidative enzymes by steaming or roasting the tea leaves of Jangwon No. 3, a new tea tree variety, is produced, and the fermentation progress of green tea Oolong tea (semi-fermented tea), black tea (fermented tea), or post-fermented tea by microbial fermentation was prepared according to the condition) (see Formulation Example 10 below).

[ comparative example 4] Yabugida (general tea tree variety) Preparation of extracts

For tea Preparation of extracts

Unfermented tea, oolong tea (semi-fermented tea), black tea (fermented tea) in the same manner as in Example 2, except that Yabugida, a general tea tree introduced variety, was used instead of the new tea tree variety (Jangwon No. 3) in Example 2 and post-fermented tea (see Formulation Example 10 below).

[ comparative example 5] Okumi-dori (general tea tree variety) Preparation of extracts

For tea Preparation of extracts

Unfermented tea, oolong tea (semi-fermented tea), black tea (fermented tea) in the same manner as in Example 2, except that Okumidori, a common tea tree introduced variety, was used instead of the new tea tree variety (Jangwon No. 3) in Example 2 ) and post-fermented tea were prepared (see Formulation Example 10 below).

[ test example 1] Comparative analysis of extract components

The extract of the new tea tree variety (Jangwon No. 3) leaf prepared in Example 1, the extract of the yabugida leaf prepared in Comparative Example 1, the extract of the Kanayamidori leaf prepared in Comparative Example 2, and the yutagamidori prepared in Comparative Example 3 The catechin and caffeine components of each leaf extract were analyzed.

First, for analysis of catechin and caffeine components, each of the extracts was dissolved in 50% methanol to make a 10,000 ppm solution, and then component analysis (High-Performance Liquid Chromatography, HPLC) (Waters) was used ( Waters, 2996 PDA detector) was carried out. The stationary phase was Kanto Chemical's Mightysil RP-18 GP 250*4.6 (5 μm) column, and the mobile phase used the composition ratio shown in Table 5 below. If necessary, analysis of catechin and caffeine components may be performed by near-infrared spectroscopy.

time (minutes) A: water B: acetonitrile 0 90 10 10 90 10 30 85 15 42 80 20 44 5 95 45 5 95 49 90 10 50 90 10

As a result, as shown in Table 6 below, the total content of catechin was 41.1% by weight based on the total weight of the extract in the extract of the new tea tree variety (Jangwon No. 3) leaf, and the extract of Yabugida leaf, which is a general tea tree introduced variety. (28.1% by weight), the extract of the leaves of Kanayamidori (26.7% by weight), and the extract of leaves of yutagamidori (26.4% by weight) were higher.

In particular, extracts from the leaves of a new tea tree variety (Jangwon No. 3) are epigallocatechin-3- O- (3- O -methyl) gallate; EGCG3”Me; 3” -O -Me-EGCG) was confirmed to be contained in an amount of 2.5% by weight based on the total weight of the extract.

Due to the difference in the total content of catechin and the content of epigallocatechin-3- O-(3-O - methyl) gallate, the extract derived from a new tea tree variety (Jangwon No. It can be predicted that the aging and anti-inflammatory effects will be better.

Item New tea tree varieties (Manor No. 3)
extract
(Example 1)
(weight%) Yabugi
extract
(Comparative Example 1)
(weight%) Kanaya Midori
extract
(Comparative Example 2)
(weight%) Yutagami Street
extract
(Comparative Example 3)
(weight%) (1) Gallocatechin
(gallocatechin, GC) 0.5 0.4 0.2 0.4 (2) epigallocatechin
(epigallocatechin, EGC) 12.7 7.7 7.6 8.4 (3) catechins
(catechin, C) 0.5 0.2 0.3 0.4 (4) epicatechin
(epicatechin, EC) 3.1 2.0 1.6 1.9 (5) epigallocatechin gallate
(epigallocatechin gallate, EGCG) 18.8 15.1 14.6 12.1 (6) Gallocatechin Gallate
(gallocatechin gallate, GCG) 0.2 0.1 0.1 0.2 (7) epicatechin gallate
(epicatechin gallate, ECG) 5.3 2.6 2.3 3.0 (8) catechin gallate
(catechin gallate, CG) N/D
(Not detected) N/D N/D N/D (1)~(8): Total content of 8 types of catechins 41.1 28.1 26.7 26.4 Content of 3" -O -Me-EGCG 2.5 0.2 0.1 0.2 Caffeine content 6.6 5.0 4.9 4.7

On the other hand, the catechin and caffeine components of the extract of the new tea tree variety (Jangwon No. 3) leaf prepared in Example 2 and the Yabugida leaf extract prepared in Comparative Example 4 were analyzed.

As a result, as shown in Table 7 below, the extract of the new tea tree variety (Jangwon No. 3) leaf of Example 2 had a higher total catechin content than the Yabugida extract of 4 in comparison. At this time, the total content of the catechin is gallic acid (GA), gallocatechin (GC), epigallocatechin (EGC), catechin (catechin, C), epigallocatechin gallate (epigallocatechin gallate) , EGCG), epicatechin (EC), gallocatechin gallate (GCG), epigallocatechin-3- O-(3-O - methyl) gallate (epigallocatechin-3- O- (3-) O -methyl) gallate; EGCG3”Me; 3” -O -Me-EGCG) and epicatechin gallate (ECG).

In addition, the content of 3” -O -Me-EGCG) in the extract of the new tea tree variety (Jangwon No. 3) leaf of Example 2 was 4.7 mg g in green tea, oolong tea (semi-fermented tea) and black tea (fermented tea), respectively. ^-1 , 3.6mg·g ^-1 , and 1.94mg·g ^-1 . That is, 3” -O -Me-EGCG contained in the leaves of a new tea tree variety (Jangwon No. 3) is changed into components such as theoflavin and theorubidine, which are catechin polymers, by oxidation reaction during processing like other catechin compounds. It was confirmed that the content of 3” -O -Me-EGCG contained in the tea was decreased.

tea
kind
tea
(tea) GA GC EGC C EGCG EC GCG 3" -O -Me-EGCG ECG total content
(mg g ^-1 ) Yabugi
(Comparative Example 4) unfermented tea 2.20 2.70 21.30 1.20 59.30 7.78 3.20 0.00 12.10 109.78 half-fermented tea 1.30 2.10 13.20 0.90 34.20 0.58 0.21 0.00 7.50 59.99 fermented tea 1.23 1.90 1.44 0.47 7.25
1.16 1.75 0.00 7.90 23.1 new tea tree
(Manor No. 3)
(Example 2) unfermented tea 3.80 2.90 22.51 1.00 66.77 8.06
2.42
4.70 14.30 126.46 half-fermented tea 2.10 1.82 16.30
0.52 42.10 1.11 1.20 3.60 9.40 78.15 fermented tea 1.92 1.20 1.05 0.33 12.94 5.82 2.86
1.94 11.75 39.81

[ test example 2] Antioxidant effect test

(One) DPPH exam

DPPH (1,1-diphenyl-2-picryl hydrazyl; 1,1-diphenyl-2-picryl hydrazyl) as a free radical in order to examine the antioxidant effect of each extract prepared in Examples 1 and 3 ), a method for evaluating antioxidant activity was performed by comparing and measuring the DPPH oxidation inhibitory efficacy through the change in absorbance caused by the reduction (oxidation of antioxidants). That is, for each of the extracts prepared in Example 1 and Comparative Examples 1-3, oxidation of DPPH was inhibited and the degree of absorbance decreased compared to the control was measured, and the concentration exhibiting an absorbance of 50% or less compared to the absorbance of the control. was evaluated as an effective antioxidant concentration.

First, 190 μl of 100 μM DPPH solution (using ethanol as a solvent), 10 μl each of the extracts prepared in Example 1 and Comparative Examples 1 to 3, and 10 μl of a positive control were added to make a reaction solution, reacted at 37° C. for 30 minutes, and then at 540 nm. Absorbance was measured. As the positive control, ascorbic acid (AsA, Vitamin C), a widely used antioxidant, was used. The DPPH analysis results of each material are shown in Table 8 below, and IC ₅₀ means the sample concentration when the absorbance is reduced by 50% by the added sample.

test substance IC ₅₀ (ppm) AsA (Vitamin C) 7.1 New tea tree variety (Jangwon No. 3) extract (Example 1) 10.2 Yabugida extract (Comparative Example 1) 35.1 Kanaya Midori Extract (Comparative Example 2) 31.5 Yutagamidori extract (Comparative Example 3) 37.7

As shown in Table 8, the extract (Example 1) of a new tea tree variety (Jangwon No. 3) exhibited a similar antioxidant effect compared to ascorbic acid, an antioxidant used as a positive control, and the general tea tree introduced variety Yabu of Comparative Examples 1 to 3 Compared with the extracts of Kida, Kanaya-midori and Yutagami-dori, it showed a significantly higher antioxidant effect.

(2) superoxide Anion scavenging activity test

In order to confirm the superoxide anion scavenging activity of the new tea tree variety (Jangwon No. 3) extract (Example 1) and the Yabugida extract (Comparative Example 1), a new tea tree variety (Jangwon No. 3) at concentrations of 10 μM and 20 μM The superoxide anion scavenging activity of the extract, the Yabugida extract and the positive control ascorbic acid (AsA, Vitamin C) was measured and compared by ESR spectrophotometer.

As a result, as shown in Figure 4, at a concentration of 10μM (or 20μM) of the new tea tree variety (Jangwon No. 3) extract or Yabugida extract It was confirmed that the superoxide anion scavenging activity was higher than that of ascorbic acid, a positive control. That is, the new tea tree variety (Jangwon No. 3) extract exhibited a scavenging activity of 54.8% at a concentration of 10 μM and 76.3% at a concentration of 20 μM, and the Yabugida extract exhibited 47.4% scavenging activity at a concentration of 10 μM and 64.9% at a concentration of 20 μM. activity, AsA exhibited a scavenging activity of 21.2% at a concentration of 20 μM.

Through these results, the DMPO-OOH signal generated without treatment with antioxidants can be strongly eliminated, and when calculated in moles, the ratio of DMPO reacting with superoxide anion is higher than the ratio of DMPO with superoxide anion. -O -Me-EGCG) reacted with superoxide anion more than about 10,000 times higher.

The superoxide anion scavenging activity of the new tea tree variety (Jangwon No. 3) extract was higher than AsA . -It is thought that it is due to the gallate group that EGCG has in common and the ortho-trihydroxy which has in the B ring.

In addition, the fact that the new tea tree variety (Jangwon No. 3) extract exhibited higher superoxide anion scavenging activity than the Yabugida extract was caused by 3" -O -Me-EGCG, a catechin component as well as the EGCG component commonly contained in tea leaves. It is considered to be due to a synergistic effect.

(3) Hydroxyl radical scavenging activity test

To confirm the hydroxyl radical scavenging activity of a new tea tree variety (Jangwon No. 3) extract (Example 1) and Yabugida extract (Comparative Example 1), a new tea tree variety (Jangwon No. 3) at concentrations of 10 μM, 25 μM and 50 μM ) extract, Yabugida extract, and ascorbic acid (AsA) as a positive control group was compared by measuring the hydroxyl radical scavenging activity of the ESR spectrophotometer (Electron Spin Resonance Spectrophotometer).

As a result, as shown in FIG. 5 , the extract of a new variety of tea tree (Jangwon No. 3) had higher scavenging activity than the Yabugida extract at a concentration of 10 μM or 25 μM. On the other hand, at a concentration of 50 μM or more, the scavenging activity between the two extracts was not significantly different, but the new tea tree variety (Jangwon No. 3) extract exhibited a hydroxyl radical scavenging activity at a level about 5 times higher than that of AsA.

Therefore, it was confirmed that the extract of a new tea tree variety (Jangwon No. 3) according to the present invention and a composition comprising the same can provide excellent antioxidant effect.

[ test example 3] Collagenase of produce deterrent measurement

The collagenase production inhibitory ability of each extract prepared in Example 1 and Comparative Examples 1 to 3 was measured by comparing it with tocopherol. Tocopherol is a representative antioxidant, a substance known to have the function of preventing skin aging by regenerating epidermal cells of the skin.

For the test, 5,000 cells/well of human fibroblasts were placed in a 96-well microtiter plate containing DMEM (Dulbecco's Modified Eagle's Media) medium containing 2.5% fetal bovine serum, and 90% Incubated until full growth. Then, after culturing in serum-free DMEM medium for 24 hours, extracts of Preparation Examples 1 and Comparative Examples 1 to 3 dissolved in serum-free DMEM medium were treated at 50 ppm concentration and tocopherol at 1x10 ^-4 molar concentration for 24 hours. Cell culture was collected.

The degree of collagenase production was measured for the collected cell culture using a commercially available collagenase measuring instrument (Amersham Pharma, USA). First, the collected cell culture solution was placed in a 96-well plate uniformly coated with the primary collagenase antibody, and the antigen-antibody reaction was performed in a thermostat for 3 hours.

After 3 hours, the chromophore-conjugated secondary collagen antibody was placed in a 96-well plate and reacted for 15 minutes again. After 15 minutes, a color-generating substance was added to induce color development at room temperature for 15 minutes, and then 1M sulfuric acid was added to stop the reaction (color development).

The absorbance of a 96-well plate having a yellow color was measured at 405 nm using an absorber, and the degree of collagenase synthesis was calculated by Equation 1 below. At this time, the reaction absorbance of the cell culture solution collected from the group not treated with the composition was used as a control. That is, the expression level of collagenase in the untreated group was set to 100, and in contrast, the expression level of collagenase in the group treated with the test substance was obtained, and the results are shown in Table 9 below.

test substance Collagenase expression level (%) untreated group 100 tocopherol 63 New tea tree variety (Jangwon No. 3) extract (Example 1) 51 Yabugida extract (Comparative Example 1) 69 Kanaya Midori Extract (Comparative Example 2) 74 Yutagamidori extract (Comparative Example 3) 76

The lower the expression level of collagenase, the higher the ability to inhibit the expression of collagenase, the less degradation of collagen in the skin, the decrease in skin elasticity is inhibited, and the amount of wrinkles generated is reduced. As shown in Table 9, the extract (Example 1) of a new tea tree variety (Jangwon No. 3) according to the present invention effectively inhibited the expression of collagenase in vitro , and more collagen than tocopherol used as a positive control. It was confirmed that the ability to inhibit the expression of genase was excellent. In particular, the extract (Example 1) of a new tea tree variety (Jangwon No. 3) according to the present invention showed more collagenase expression than the Yabugida, Kanayamidori and Yutagamidori extracts of the general tea tree introduced varieties of Comparative Examples 1 to 3 By effectively inhibiting it, it was confirmed that the collagen decomposition in the skin was suppressed, and the skin elasticity enhancement effect and the wrinkle reduction effect were excellent.

Therefore, it was confirmed that the extract of a new tea tree variety (Jangwon No. 3) according to the present invention and a composition comprising the same can provide an excellent anti-aging effect.

[ test example 4] Anti-inflammatory effect test

1. Inhibitory effect of prostaglandin production

The skin anti-inflammatory effect of each extract prepared in Example 1 and Comparative Examples 1 to 3 was evaluated as the effect of inhibiting the production of prostaglandins. Anti-inflammatory effects were measured on macrophages using each of the extracts. First, the cyclooxygenase (COX) activity remaining in the cells was irreversibly inhibited by adding aspirin to the macrophages collected from the abdominal cavity of the mouse so that the final concentration was 500M. Then, 100 μl of the suspension was put into each well of a 96-well cell culture tube and cultured for 2 hours in an incubator under 5% CO ₂ and 37° C. conditions to attach macrophages to the surface of the vessel. Then, the adherent macrophages were washed 3 times with PBS and then used for anti-inflammatory effect test. After culturing for 12 hours by adding RPMI medium containing LPS (Lipopolysaccharides) at 1% (w/v) to 5x10 ⁴ cells/ml of the cultured macrophages, the production of prostaglandins was induced and Example 1 and Comparative Example 1 Each of the extracts prepared in ~ 3 was treated with 100 μl and the prostaglandins released were quantified using an enzyme immunoassay (ELISA). At this time, the prostaglandin production inhibitory activity (%) of each extract prepared in Example 1 and Comparative Examples 1 to 3 is shown in Table 10 below.

test substance Prostaglandin inhibitory ability (%) Untreated group (NT) 0 Control group (aspirin treatment group) 58.6 Tea tree new variety (Jangwon No. 3) extract (Example 1) 56.9 Yabugida extract (Comparative Example 1) 37.5 Kanaya Midori Extract (Comparative Example 2) 27.4 Yutagamidori extract (Comparative Example 3) 33.8

As shown in Table 10, it was found that the extract (Example 1) of a new tea tree variety (Jangwon No. 3) had a very high prostaglandin production inhibitory effect like the control treated with aspirin, and the general tea tree of Comparative Examples 1 to 3 It was found that the inhibitory effect on prostaglandin production was significantly higher than that of the introduced varieties Yabugida, Kanayamidori and Yutagamidori extracts.

Therefore, the extract of a new tea tree variety (Jangwon No. 3) according to the present invention and a composition comprising the same have an anti-inflammatory effect by suppressing the expression of prostaglandin, a skin inflammatory factor, and further improve skin acne and skin allergies by alleviating skin troubles. was able to confirm that

2. IL-8 production inhibitory effect

The skin anti-inflammatory effect of each extract prepared in Example 1 and Comparative Examples 1 to 3 was evaluated as an inflammatory factor, Interleukin-8 (IL-8) production inhibitory effect. First, one day before the experiment, normal human skin keratinocytes (NHEK, obtained from Lonza) were dispensed in a 96-well plate at a volume of 5x10 ⁴ cells/well and then 37°C, 5% CO ₂ Incubator (incubator) ) incubated for 24 hours. After 24 hours, the cells were washed twice with PBS and replaced with serum free keratinocyte basement media (KBM). Each of the extracts prepared in Example 1 and Comparative Examples 1 to 3 was treated in each well for 30 minutes and reacted for 30 minutes, PGSA (10㎍ / ㎖), PGSA (50㎍ / ㎖), PGSA (50 μg/ml)+LPS (1 μg/ml) were treated respectively. Here, PGSA (peptidoglycan from S. aureus ) is a peptidoglycan extracted from Staphylococcus aureus, a major component of the cell wall of Gram-positive (+) bacteria, and the cell membrane components of bacteria are known to cause inflammation, In particular, in the case of Staphylococcus aureus, it has been reported that about 90% of atopic patients cause secondary infection by this fungus. After culturing in an incubator at 37° C., 5% CO ₂ for 24 hours, the culture medium was taken and ELISA for interleukin-8 was performed, and the results are shown in Table 11 below. For ELISA, the experimental method of the manufacturer (BD science) was used.

test substance IL-8 secretion (pg/ml) Untreated group (NT) 935.12 PGSA 10 μg/ml 4725.64 PGSA 10㎍/㎖ + 25ppm of new tea tree variety (Jangwon No. 3) extract (Example 1) 1503.29 PGSA 10㎍/㎖ + new tea tree variety (Jangwon No. 3) extract (Example 1) 50ppm 1002.57 PGSA 10㎍/㎖ + Yabugida extract (Comparative Example 1) 25ppm 2765.42 PGSA 10㎍/㎖ + Yabugida extract (Comparative Example 1) 50ppm 2185.22 PGSA 10㎍/㎖ + Kanayamidori extract (Comparative Example 2) 25ppm 2864.77 PGSA 10㎍/㎖ + Kanayamidori extract (Comparative Example 2) 50ppm 2186.59 PGSA 10㎍/㎖ + Yutagamidori extract (Comparative Example 3) 25ppm 2869.10 PGSA 10㎍/㎖ + Yutagamidori extract (Comparative Example 3) 50ppm 2105.73

As shown in Table 11, it was found that the extract (Example 1) of a new tea tree variety (Jangwon No. 3) had a very high effect of inhibiting the secretion of IL-8 increased by PGSA, an inflammation-inducing stimulus, Comparative Examples 1 to 3 It was found that it significantly reduced and inhibited the secretion of IL-8 compared to the extracts of Yabugida, Kanayamidori and Yutagamidori of general tea tree introduced varieties.

Therefore, the extract of a new tea tree variety (Jangwon No. 3) according to the present invention and a composition comprising the same have an anti-inflammatory effect by suppressing the secretion of IL-8, which is a stimulant that causes skin inflammation, and furthermore, alleviates skin troubles to relieve skin acne and skin allergies. could be improved.

[ test example 5] cancer cellism proliferation inhibition test

An extract of a new tea tree variety (Jangwon No. 3) leaf (hereinafter referred to as a new tea tree variety (Jangwon No. 3) extract), or 3" -O -Me-EGCG isolated from the extract, a cancer cell line lung cancer cell line (A549), kidney cancer Cell line (ACHN), colon cancer cell line (HCT15), prostate cancer cell line (LNCaP) and breast cancer cell line (MCF-7) were treated, and then incubated for 36 hours at 37° C., 5% CO ₂ incubator for 36 hours. After incubation, the cancer cell proliferation inhibitory effect of the new tea tree variety (Jangwon No. 3) extract or 3" -O -Me-EGCG on the cell line was confirmed by MTT assay, but the negative control treated with 0.1% DMSO As a reference, the relative cancer cell proliferation rate was measured.

As a result, as shown in Table 12 below,

(i) When each cancer cell line is treated with an extract of a new tea tree variety (Jangwon No. 3) or 3" -O -Me-EGCG at a concentration of 100 μg·mL ^-1 , the cancer cell proliferation rate of 50% or less in all cancer cell lines (survival rate) was shown,

When the extract of a new tea tree cultivar (Jangwon No. 3) was treated at a concentration of 200 μg·mL ^-1 to each cancer cell line, the lung cancer cell line (A549), the prostate cancer cell line (LNCaP), and the breast cancer cell line (MCF-7) The cancer cell proliferation rates of 18%, 24% and 16% were shown, confirming that the extract of a new variety of tea tree (Jangwon No. 3) had an inhibitory effect on cancer cell proliferation.

(ii) In addition, as a result of treating each cancer cell line with an extract of a new tea tree variety (Jangwon No. 3), or 3" -O -Me-EGCG, the cancer cell proliferation was inhibited in a concentration-dependent manner for all cancer cell lines,

In particular, the prostate cancer cell line (LNCaP) was treated with 3" -O -Me-EGCG derived from the extract of a new variety of tea tree (Jangwon No. 3) at concentrations of 50㎍·mL ^-1 , 100㎍·mL ^-1 , and 200㎍·mL ^-1 . In this case, the proliferation rate of the prostate cancer cell line was about 46%, 37%, and 20%, respectively, indicating a high cancer cell proliferation inhibitory effect. " -O -Me-EGCG showed a high cancer cell proliferation inhibitory effect,

When the lung cancer cell line (A549) was treated with 3" -O -Me-EGCG derived from the extract of a new tea tree variety (Jangwon No. 3) at concentrations of 50 μg·mL ^-1 , 100 μg·mL ^-1 , and 200 μg·mL ^-1 , cancer cell proliferation rates were approximately 41%, 20%, and 16%, respectively, indicating that the lung cancer cell line (A549) exhibited higher cancer cell proliferation inhibitory efficacy than the prostate cancer cell line (LNCaP) and breast cancer cell line (MCF-7).

sample density
(μg/mL) Cancer cell proliferation rate (mean±SD) A549 ACHN LNCaP HCT15 MCF-7 Extract of a new tea tree variety (Jangwon No. 3) 200 18.0±8.0 32.0±4.0 24.0±5.0 25.0±4.0 16.0±2.0 100 21.0±6.0 46.0±6.0 35.0±4.0 36.0±2.0 29.0±4.0 50 54.0±7.0 61.0±6.0 41.0±6.0 51.0±4.0 58.0±5.0 3" -O -Me-EGCG 200 16.0±6.0 40.0±3.0 20.0±3.0 36.0±2.0 18.0±2.0 100 20.0±5.0 49.0±5.0 37.0±4.0 42.0±1.0 31.0±1.0 50 41.0±3.0 63.0±3.0 46.0±3.0 60.0±3.0 62.0±2.0

[ test example 6] Example 2 and comparative example Sensory evaluation of extracts prepared in 4-5

Green tea (non-fermented tea), oolong tea (semi-fermented tea), black tea (fermented tea), or black tea (post-fermented tea) have different characteristics such as shape, color, aroma, color, and taste. In particular, in oolong tea (semi-fermented tea), black tea (fermented tea), or black tea (post-fermented tea), palatability changes such as aroma, color, and taste occur greatly depending on the fermentation progress, and the ingredients contained also change. In order to compare changes in palatability and functionality according to the processing method according to the degree of fermentation for tea leaves through extreme shading, the extract of the new tea tree variety (Jangwon No. 3) of Example 2, Yabugida (Japanese variety) of Comparative Example 4 For the extract and the okumidori (Japanese variety) extract of Comparative Example 5, appearance evaluation (shape, color; out of 40 points) and internal quality evaluation (scent, color, taste) in order to examine the palatability and functionality as a tea ; out of 60 points) was performed.

As a result, as shown in Table 13 below, regardless of the processing method in the sensory evaluation, the new tea tree variety (Jangwon No. 3) showed higher palatability in all semi-fermented tea, fermented tea, and post-fermented tea than Yabugida and Okumidori. It has been shown to have excellent properties in the manufacture of various kinds of tea, so it was evaluated to have very high utilization.

product
(Processing type) kind Appearance evaluation
(40 points) internal evaluation
(60 points) Sum
(100 points) shape colour Scent search taste green tea
(Non-fermented tea) Yabugi 18 18 19 18 18 91 Okumi-dori 17 18 18 18 18 89 new tea tree
(Manor No. 3) 18 19 18 18 19 92 oolong tea
(Semi-fermented tea) Yabugi 17 16 16 15 16 80 Okumi-dori 17 17 16 17 16 83 new tea tree
(Manor No. 3) 18 18 19 19 19 93 black tea
(fermented tea) Yabugi 17 16 15 16 14 78 Okumi-dori 17 17 15 16 15 80 new tea tree
(Manor No. 3) 18 18 19 19 19 93 black tea
(Post-fermented tea) Yabugi 17 17 15 17 15 81 Okumi-dori 17 16 15 16 14 78 new tea tree
(Manor No. 3) 18 19 19 19 18 93

As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereby. something to do. Accordingly, it is intended that the substantial scope of the present invention be defined by the appended claims and their equivalents.

In addition, the formulation example comprising the composition containing the extract of the new tea tree variety (Jangwon No. 3) of the present invention will be described in more detail below, but it will be clear that the scope of the present invention is not limited thereby.

[ Formulation example 1] Manufacture of nutrient lotion

Nutrient lotion was prepared by a conventional method according to the composition shown in Table 14 (unit: wt%).

ingredient content Purified water remaining amount glycerin 8.0 butylene glycol 4.0 Hyaluronic Acid Extract 5.0 beta glucan 7.0 Carbomer 0.1 Tea tree new variety (Jangwon No. 3) extract (Example 1 or 2) 1.0 Caprylic/Capric Triglycerides 8.0 squalane 5.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 cetearyl alcohol 1.0 triethanolamine 0.1

[ Formulation example 2] Manufacture of nourishing cream

Nutrient creams were prepared in a conventional manner according to the composition shown in Table 15 (unit: wt%).

ingredient content Purified water remaining amount glycerin 3.0 butylene glycol 3.0 liquid paraffin 7.0 beta glucan 7.0 Carbomer 0.1 Tea tree new variety (Jangwon No. 3) extract (Example 1) 5.0 Caprylic/Capric Triglycerides 3.0 squalane 5.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 Polysorbate 60 1.2 triethanolamine 0.1

[ Formulation example 3] Preparation of massage cream

A massage cream was prepared in a conventional manner according to the composition shown in Table 16 (unit: wt%).

ingredient content Purified water remaining amount glycerin 8.0 butylene glycol 4.0 liquid paraffin 45.0 beta glucan 7.0 Carbomer 0.1 Tea tree new variety (Jangwon No. 3) extract (Example 1) 2.0 Caprylic/Capric Triglycerides 3.0 beeswax 4.0 cetearyl glucoside 1.5 Sorbitan Sesquioleate 0.9 Vaseline 3.0 paraffin 1.5

[ Formulation example 4] Manufacture of the pack

A pack was prepared in a conventional manner according to the composition shown in Table 17 (unit: wt%).

ingredient content Purified water remaining amount glycerin 4.0 polyvinyl alcohol 15.0 Hyaluronic Acid Extract 5.0 beta glucan 7.0 allantoin 0.1 Tea tree new variety (Jangwon No. 3) extract (Example 1) 0.5 nonylphenyl ether 0.4 Polysorbate 60 1.2 ethanol 6.0

[ Formulation example 5] Preparation of ointment

An ointment was prepared in a conventional manner according to the composition shown in Table 18 (unit: wt%).

ingredient content Purified water remaining amount glycerin 8.0 butylene glycol 4.0 liquid paraffin 15.0 beta glucan 7.0 Carbomer 0.1 Tea tree new variety (Jangwon No. 3) extract (Example 1) 1.0 Caprylic/Capric Triglycerides 3.0 squalane 1.0 cetearyl glucoside 1.5 Sorbitan Stearate 0.4 cetearyl alcohol 1.0 beeswax 4.0

[ Formulation example 6] Preparation of soft capsules

The extract (Example 1) of a new tea tree variety (Jangwon No. 3) (Example 1) 80 mg, vitamin E 9 mg, vitamin C 9 mg, palm oil 2 mg, hydrogenated vegetable oil 8 mg, yellow wax 4 mg and lecithin 9 mg are mixed, and mixed according to a conventional method to fill the soft capsule. liquid was prepared. Soft capsules were prepared by filling 400 mg per capsule. Separately, a soft capsule sheet was prepared at a ratio of 66 parts by weight of gelatin, 24 parts by weight of glycerin, and 10 parts by weight of sorbitol solution, and the filling solution was filled to prepare a soft capsule containing 400 mg of the composition according to the present invention.

[ Formulation example 7] Preparation of tablets

An extract (Example 1) of a new tea tree variety (Jangwon No. 3) (Example 1) 80 mg, vitamin E 9 mg, vitamin C 9 mg, galactooligosaccharide 200 mg, lactose 60 mg and maltose 140 mg were mixed and granulated using a fluidized bed dryer, followed by sugar ester (sugar ester) 6 mg was added. 504 mg of these compositions were compressed in a conventional manner to prepare tablets.

[ Formulation example 8] Manufacture of drinks

After mixing 80 mg of extract (Example 1) of a new tea tree variety (Jangwon No. 3), vitamin E 9 mg, vitamin C 9 mg, glucose 10 g, citric acid 0.6 g, and liquid oligosaccharide 25 g, 300 ml of purified water was added, and 200 ml of each bottle was filled to the brim. After filling the bottle, it was sterilized at 130° C. for 4 to 5 seconds to prepare a beverage.

[ Formulation example 9] Preparation of granules

80 mg of extract (Example 1) of a new tea tree variety (Jangwon No. 3), vitamin E 9 mg, vitamin C 9 mg, anhydrous crystalline glucose 250 mg, and starch 550 mg were mixed, formed into granules using a fluidized bed granulator, and then filled in a bag was prepared.

[ Formulation example 10] Preparation of tea composition

In order to suppress the activity of polyphenol oxidase, which decomposes polyphenols, in tea leaves of a new tea tree variety (Jangwon No. 3), a steamer operated by a boiler was used to maintain the used tea leaf temperature at 90°C for about 30 seconds. . After drying sufficiently so that the moisture content is 6% or less, it was ground to a particle size of 0.5 to 4 mm using a grinder, and then this was prepared into a 1.0 g tea bag.

Korea Institute of Bioscience and Biotechnology KCTC12213BP 20120514

Claims (24)

epigallocatechin-3- O -(3- O -methyl) gallate; EGCG3”Me; 3”-O - Me-EGCG), epigallocatechin-3- O Skin elasticity improvement and skin elasticity containing extract of Camellia sinensis including locatechin (epigallocatechin, EGC), epigallocatechin gallate (EGCG) and epicatechin gallate (ECG) as an active ingredient As a composition for reducing skin wrinkles,
The extract contains, based on the total weight of the extract, 1.25% by weight or more of epigallocatechin-3- O-(3-O - methyl) gallate, 6.35-25.4% by weight of epigallocatechin, 9.4-37.6% by weight Epigallocatechin gallate and 2.65 to 10.6% by weight of epicatechin gallate;
The tea tree is a composition for enhancing skin elasticity and reducing skin wrinkles, characterized in that it is Camellia sinensis Jangwon No. 3, which was seeded under the accession number KCTC (Korean Collection for Type Cultures) 12213BP. The skin elasticity enhancement and A composition for reducing skin wrinkles. The method according to claim 1, wherein the extract contains 9.53 to 19.05% by weight of epigallocatechin, 16.9 to 23.5% by weight of epigallocatechin gallate, and 3.98 to 7.95% by weight of epicatechin gallate, based on the total weight of the extract. A composition for enhancing skin elasticity and reducing skin wrinkles, comprising: The composition for enhancing skin elasticity and reducing skin wrinkles according to claim 1, wherein the extract is at least one selected from the group consisting of flowers, leaves, fruits, stems and roots of tea tree. The composition of claim 1, wherein the extract is contained in an amount of 0.01 to 20% by weight based on the total weight of the composition. The composition for enhancing skin elasticity and reducing skin wrinkles according to claim 1, wherein the extract has an activity to inhibit the expression of collagenase in skin cells. According to claim 1, wherein the extract is characterized in that the ethanol extract, skin elasticity enhancement and skin wrinkle reduction composition. According to claim 7, wherein the extract is obtained by collecting the leaves of the tea tree, steaming with a steamer, drying in a dryer, pulverizing with a grinder, adding an aqueous ethanol solution to reflux extraction, filtering, and then concentrating under reduced pressure. A composition for enhancing skin elasticity and reducing skin wrinkles, characterized in that it will. The skin according to claim 8, wherein the leaf is collected from the upper first to the third leaf of the shoot based on the period when the opening degree of the first five leaves is 70% during the first water tea (early May) period. A composition for improving elasticity and reducing skin wrinkles. delete [10] The composition according to any one of claims 1 to 9, wherein the composition is a pharmaceutical composition, a cosmetic composition, or a health functional food composition. delete delete delete delete delete delete delete delete delete delete delete delete delete

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