KR102421986B1 - Method and kit for measuring the amount of sebum - Google Patents

Abstract

The present invention relates to a method for measuring an amount of sebum, wherein a kit for measuring sebum according to an embodiment of the present invention comprises a compound represented by chemical formula 1. [Chemical formula 1] When the amount of sebum is determined by the method of the present invention, the amount of sebum can be determined simply and accurately.

Description

Method and kit for measuring the amount of sebum

The present invention relates to methods and kits for measuring the amount of sebum.

Sebum is a secretion from the sebaceous glands in the skin, and the liquid fat secreted from the sebaceous glands passes through the hair follicles and is discharged from the hair follicles to prevent drying of the skin surface.脣溝), armpits, chest and abdomen (胸背部), and is widely distributed in the groin. Sebum secretion, especially during puberty, increases rapidly, because during puberty, male hormones are secreted excessively and stimulate the sebaceous glands attached to the hair follicles. If such sebum is not properly discharged and removed, it accumulates in the pores and oxidizes with air, resulting in black masses (blackheads) appearing on the skin surface, which can be a great stress for adolescents who are interested in their appearance and are sensitive. .

On the other hand, the device for determining sebum is a sebumeter, which detects the amount of sebum using the reflected wavelength. However, since such a device is expensive, a device and a sebum detection method to replace it are needed.

In the present invention, a method for measuring the amount of sebum only by detecting the color after simple staining of sebum was devised.

Application No. 10-2013-0158925

It is an object of the present invention to provide a kit for measuring sebum and a method and kit for determining the amount of sebum using the same.

Accordingly, the present invention provides a kit for measuring sebum comprising a compound represented by the following formula (1).

[Formula 1]

In another aspect of the present invention, there is provided a method for determining the amount of sebum comprising; staining the sebum isolated from the subject with a compound represented by the following formula (1).

[Formula 1]

The sebum separated from the individual may be collected using an adsorbent.

In addition, the method may further include determining the amount of the measured sebum using Equation 1 below to measure the amount of the dyed sebum.

[Equation 1]

Casual sebum level = Red-((Green + Blue)/2)

Red: The value of the red component in the RGB cube model.

Green: The value of the green component in the cube model of RGB.

Blue: The value of the blue component in the cube model of RGB.

In addition, the kit for measuring sebum according to an embodiment of the present invention, an adsorbent for absorbing skin wastes in contact with the skin; A compound represented by the following formula (1) for dyeing the adsorbent; and an analysis device for determining the amount of sebum using the adsorbent dyed with the compound, wherein the analysis device includes: a sample receiving unit for accommodating the adsorbent; a light irradiator for irradiating light having an RGB component toward the adsorbent; a color sensor for detecting the light reflected or emitted by the adsorbent; and a processor that determines the amount of sebum by using the component values in the RGB cube model detected by the color sensor.

[Formula 1]

When determining the amount of sebum by the method of the present invention, it is possible to determine the amount of sebum simply and accurately.

1 is a view showing the results of collecting sebum using oil blotting paper.
Figure 2 is a view showing the results when sebum is collected using the plastic film of the present invention.
3 is a result of measuring the amount of sebum after digitizing the color density and converting it into RGB components using Image J software (Image J) of the present invention.
Fig. 4a shows the difference in RGB components for each wavelength of light, and Fig. 4b shows that the color sensor according to an embodiment of the present invention detects the RGB components of light, digitizes the measured data, and transmits the digitized data to the processor.
5A is a conceptual diagram illustrating a kit for measuring sebum according to an embodiment of the present invention.
Figure 5b is a conceptual diagram expressing a kit for measuring sebum according to another embodiment of the present invention.
6 is a flowchart of a method for measuring sebum according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail by way of embodiments of the present invention with reference to the accompanying drawings. However, the following embodiments are presented as examples for the present invention, and when it is determined that detailed descriptions of well-known techniques or configurations known to those skilled in the art may unnecessarily obscure the gist of the present invention, the detailed description may be omitted, and , the present invention is not limited thereby. Various modifications and applications of the present invention are possible within the scope of equivalents interpreted therefrom and the description of the claims to be described later.

In addition, the terms used in this specification are terms used to properly express preferred embodiments of the present invention, which may vary depending on the intention of a user or operator or customs in the field to which the present invention belongs. Accordingly, definitions of these terms should be made based on the content throughout this specification. Throughout the specification, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

The color sensors 21 and 22 are detectors that detect the wavelength of the light emitted or reflected by the object to determine the color. A MOS-type camera by CCD or other light-receiving element is used, and the wavelength is converted into voltage and the output is processed to determine the color.

In one aspect, therefore, the present invention provides a kit for measuring sebum comprising a compound represented by the following formula (1).

[Formula 1]

The compound represented by Formula 1 is ORO (Oil Red O), which is used as a sample for staining fat. The compound can absorb a wavelength of 518 nm to dye a subject red.

In another aspect of the present invention, there is provided a method for determining the amount of sebum comprising; staining the sebum isolated from the subject with a compound represented by the following formula (1).

[Formula 1]

The sebum may be collected using the adsorbents 31 and 32, and if it is collected by other methods, staining with the compound of Formula 1 may not be performed well.

In one embodiment, the adsorbents 31 and 32 may be a plastic film in which the absorption rate is increased by porous chemical treatment of polypropylene made by refining petroleum. In another embodiment, the adsorbents 31 and 32 may be paper using hemp. Although the film has excellent absorbency, paper can also absorb sebum.

In addition, the color sensors 21 and 22 measure the color of sebum dyed with the compound of Formula 1 in the present invention, and the processor 10 uses the data obtained from the color sensors 21 and 22 to obtain a color value. can be obtained The processor 10 determines the amount of sebum using the obtained value.

The processor 10 determines the amount of sebum by a formula using the RGB color model.

The RGB color model is a method of expressing 　 colors using the three primary colors of light. Red (RED), 　Green (GREEN), 　Blue (BLUE) is used to mix colors using three types of light sources.

Like the 'RGB cube model', a color can be expressed as a point in a three-dimensional and Cartesian coordinate system using the three primary colors as a reference line.

For example, 　black is a state in which all three light sources have no light, and it can be expressed as 　coordinates (0,0,0).

If the maximum value of each light source is 255, red is expressed as the coordinates (255,0,0), only the RED light source has the maximum value, and the GREEN light source and the BLUE light source mean there is no light.

The processor 10 may calculate the RGB value and measure the amount of casual sebum. In the present invention, the amount of casual sebum may be defined by Equation 1 below, and the amount of casual sebum has a very high correlation with the amount of sebum that is actually present.

[Equation 1]

Casual sebum level = Red-((Green + Blue)/2)

Red: The value of the red component in the RGB cube model.

Green: The value of the green component in the cube model of RGB.

Blue: The value of the blue component in the cube model of RGB.

Hereinafter, the present invention will be described in detail by way of Examples.

[Example 1] Quantitative effect of sebum according to sebum collection method

1. When sebum is collected using oil blotting paper

Four types of commercial oil blotting paper were used to collect facial skin sebum (see Fig. 1), and for detecting the collected sebum, ORO (Oil Red O) staining of the present invention was performed. Among the facial skin regions, the well-known “T zone” was selected as a region with a lot of sebum. More specifically, samples and controls (negative control: no sebum) at 0 minutes, 30 minutes and 1 hour after face washing and ORO staining were stained after sebum collection was performed using commercial oil blotting paper.

As shown in FIG. 1 , it was confirmed that the quantification of casual sebum using chromaticity caused by red or pink background color noise was difficult.

2. When sebum is collected using plastic film

After collecting sebum using a plastic film (PVC), ORO staining was performed in the same manner as in Example 1-1. The results are shown in FIG. 2 .

As shown in FIG. 2 , a color difference between the ORO-stained plastic film without sebum and the plastic film with sebum could be detected. It was confirmed that the color density of the plastic film was higher in ORO dyeing with sebum after 1 hour than in samples dyed 0 or 30 minutes after washing.

[Example 2] Calculation of casual sebum level of the present invention

Based on the result of collecting sebum with the plastic film, the color density was digitized and converted into RGB components using Image J software (Image J).

The visual change of the intensity of each RGB component for different stained sebum after washing the face is shown in Fig. 3a, and it was confirmed that the color intensity of the dyed ORO (pink) was increased.

The above results confirmed that there was a correlation with the intensity conversion value of RGB components according to sebum recovery time when R ² = 0.9065 in all three color components (Fig. 3b).

The brightness conversion value of the RGB component (y-axis) was obtained by subtracting the average value of the green and blue components from the red component value, and this value was referred to as "casual sebum level".

[Equation 1]

Casual sebum level = Red-((Green + Blue)/2)

[Example 3] Comparison between the conventional method and the method for measuring sebum of the present invention

In order to verify the accuracy of the sebum amount measurement method of the present invention, the existing sebumeter® was used.

Specifically, sebum collected using a PVC film in the t zone after 0, 30, and 1 hour after washing was digitized by dyeing. After that, the amount of casual sebum was measured using a sebumeter® in the same procedure. As a result, it was confirmed that the correlation between the intensity of the ORO-stained color and the physimeter value was 0.946, 0.996, and 0.994 in the three samples, respectively (see Table 1 below). Therefore, the collection method using a plastic film and ORO staining for quantitative measurement of sebum is effective in determining the daily sebum level.

[Table 1]

Fig. 4a shows the difference in RGB components for each wavelength of light, and Fig. 4b shows how the color sensor according to an embodiment of the present invention detects the RGB components of light and then digitizes the measured data and transmits the digitized data to the processor. 5A is a conceptual diagram illustrating a kit for measuring sebum according to an embodiment of the present invention. Figure 5b is a conceptual diagram expressing a kit for measuring sebum according to another embodiment of the present invention.

4A to 5B, the kit for measuring sebum according to an embodiment of the present invention includes adsorbents 31 and 32 for absorbing skin wastes in contact with the skin; A compound represented by the following formula (1) for dyeing the adsorbents (31, 32); and an analysis device for determining the amount of sebum using the adsorbents 31 and 32 dyed with the compound, wherein the analysis device includes: sample receiving units 41 and 42 for accommodating the adsorbents 31 and 32; light irradiation units 51 and 52 for irradiating light having RGB components toward the adsorbents 31 and 32; color sensors 21 and 22 for detecting the light reflected or emitted by the adsorbents 31 and 32; and a processor 10 that determines the amount of sebum by using the ratio of the amount of R·G·B detected by the color sensors 21 and 22 or the component value in the RGB cube model.

The color sensors 21 and 22 detect light R (red), G (green), and B (blue), which are three primary colors of light among sensors (optical sensors).

The light irradiating units 51 and 52 irradiate the light having an RGB component to the adsorbents 31 and 32 . The light irradiation units 51 and 52 irradiate light having RGB components to the sample receiving units 41 and 42 .

The processor 10 calculates the amount of sebum using Equation (1).

A kit for measuring sebum according to an embodiment of the present invention includes: adsorbents 31 and 32 for absorbing skin wastes in contact with the skin; a compound for dyeing the adsorbents 31 and 32; and an analysis device for determining the amount of sebum using the adsorbents 31 and 32 dyed with the compound, wherein the analysis device includes: sample receiving units 41 and 42 for accommodating the adsorbents 31 and 32; light irradiation units 51 and 52 for irradiating light having RGB components toward the sample receiving units 41 and 42; color sensors 21 and 22 for detecting the light reflected or emitted by the adsorbents 31 and 32; And the amount of sebum is measured by using the ratio of the amount of R·G·B detected by the color sensors 21 and 22 or the component value in the RGB cube model.

5A and 5B have different positions of the light irradiator and the blocking member blocking the color sensor from detecting the light directly irradiated from the light irradiator.

According to the exemplary embodiment of FIG. 5A , a portion of the light irradiated from the lower portion of the sample receiving unit 41 is absorbed by the adsorbent 31 . The remaining wavelengths that are not absorbed by the adsorbent 31 are radiated to the color sensor 21 . The color sensor 21 detects the light passing through the adsorbent 31 . The sample receiving part 41 may be provided with a blocking member 61 capable of reflecting or absorbing the light that does not pass through the adsorbent 31 among the light irradiated from the bottom, that is, direct light.

In another embodiment according to FIG. 5B , a portion of the light irradiated by the light irradiator 52 is absorbed by the adsorbent 32 . The remaining wavelengths that are not absorbed by the adsorbent 32 are reflected by the color sensor 22 . The color sensor 22 detects the light reflected by the adsorbent. A blocking member 62 that reflects or absorbs light directly irradiated from the light irradiator 52 is positioned around the color sensor 22 . A plurality of light irradiation units 52 may be disposed, and the plurality of light irradiation units 52 are inclined toward the adsorbent 32 or the sample receiving unit 42 .

6 is a flowchart of a method for measuring sebum according to an embodiment of the present invention.

A method for determining the amount of sebum according to an embodiment of the present invention includes: dyeing the adsorbents 31 and 32 after wiping the skin with a compound (S100); irradiating light having RGB components to the adsorbents 31 and 32 (S200); a step of detecting, by the color sensors 21 and 22, the reflected light reflected or emitted by the adsorbents 31 and 32 (S300); and determining the amount of sebum by using the ratio of the R·G·B quantity detected by the processor 10 or the component value in the RGB cube model (S400). In the step of determining the amount of sebum, Equation 1 is used.

Claims (10)

a compound represented by Formula 1;
an adsorbent dyed by the compound and wiping the skin;
a light irradiator for irradiating light having an RGB component to the adsorbent;
a color sensor for detecting the reflected light reflected or emitted by the adsorbent; and
A kit for measuring sebum comprising a processor for determining the amount of sebum based on the degree of dyeing by using the component values in the RGB cube model detected by the color sensor.
[Formula 1]

Dyeing sebum isolated from the subject with a compound represented by the following formula (1);
irradiating light having an RGB component toward the dyed sebum by a light irradiator;
detecting, by a color sensor, light reflected or emitted by the dyed sebum; and
Based on the data obtained by the color sensor, the method for determining the amount of sebum comprising the step of determining, by a processor, the amount of the measured sebum using Equation 1 below.
[Equation 1]
Casual sebum level = Red-((Green + Blue)/2)
Red: The value of the red component in the RGB cube model.
Green: The value of the green component in the cube model in RGB.
Blue: The value of the blue component in the cube model of RGB.
A method for determining the amount of sebum containing.
[Formula 1]

3. The method of claim 2,
A method for determining the amount of sebum, the sebum separated from the individual is collected using an adsorbent. delete delete The method of claim 1,
The processor is a kit for measuring sebum for determining the amount of sebum using Equation 1 below.
[Equation 1]
Casual sebum level = Red-((Green + Blue)/2)
Red: The value of the red component in the RGB cube model.
Green: The value of the green component in the cube model in RGB.
Blue: The value of the blue component in the cube model of RGB. an adsorbent that comes into contact with the skin and absorbs skin wastes;
A compound represented by the following formula (1) for dyeing the adsorbent; and
An analysis device for determining the amount of sebum using the adsorbent dyed with the compound,
The analyzer is
a sample receiving unit accommodating the adsorbent;
a light irradiator for irradiating light having an RGB component toward the adsorbent;
a color sensor for detecting the light reflected or emitted by the adsorbent; and
A sebum measurement kit comprising a processor for determining the amount of sebum by using the component values in the RGB cube model detected by the color sensor.
[Formula 1]

8. The method of claim 7,
The processor is a kit using Equation 1 below.
[Equation 1]
Casual sebum level = Red-((Green + Blue)/2)
Red: The value of the red component in the RGB cube model.
Green: The value of the green component in the cube model in RGB.
Blue: The value of the blue component in the cube model of RGB. an adsorbent that comes into contact with the skin and absorbs skin wastes;
Oil Red O for dyeing the adsorbent; and
An analyzer for judging the amount of sebum using the adsorbent dyed with the Oil Red O,
The analyzer is
a sample receiving unit accommodating the adsorbent;
a light irradiation unit irradiating light having RGB components toward the sample receiving unit;
a color sensor for detecting the light reflected or emitted by the adsorbent; and
A sebum measurement kit comprising a processor for determining the amount of sebum by using the component values in the RGB cube model detected by the color sensor. 10. The method of claim 9,
The processor is a kit using Equation 1 below.
[Equation 1]
Casual sebum level = Red-((Green + Blue)/2)
Red: The value of the red component in the RGB cube model.
Green: The value of the green component in the cube model in RGB.
Blue: The value of the blue component in the cube model of RGB.

Images