KR102413828B1 - Method of Manufacturing and Taking Grub Powder - Google Patents

Abstract

The disclosed method for producing slug powder includes a primary drying step of washing mature slugs and lowering the moisture content of the slugs washed by a low temperature vacuum drying method using a microwave to a set initial moisture content; a secondary drying step of drying the slugs provided in the primary drying step at a preset drying temperature and drying time; and a grinding step of pulverizing the slugs provided in the secondary drying step to prepare slug powder.

Description

Method of Manufacturing and Taking Grub Powder

The present invention (Disclosure) relates to a method for preparing and taking a slug powder, and more particularly, to a method for preparing and taking a slug powder capable of repairing liver damaged cells.

Herein, background information related to the present invention is provided, which does not necessarily imply prior art (This section provides background information related to the present disclosure which is not necessarily prior art).

In order to actively respond to the import of foreign health functional food by actively using Korean agricultural products as raw materials for health functional food, and to foster the domestic health functional food industry, it is necessary to discover excellence in health functionality and stability of Korean agricultural products.

It was analyzed that the growth of health functional food production continued, reflecting the consumer needs of various classes seeking new functionalities as interest in health increased despite the economic downturn at home and abroad.

As a result of analyzing the status of accreditation of functional ingredients for health functional food in 2013, it was found that while the accreditation of imported raw materials decreased while the accreditation of domestic manufacturing raw materials increased rapidly, while the overall accreditation of individually accredited health functional food was declining.

By function, products related to immune function improvement accounted for the highest share at 25%, followed by blood circulation improvement (22%), antioxidant (21%), nutrient supplementation (7%), and intestinal health (5%) products.

Meanwhile, the domestic insect market has nearly doubled in four years from 168 billion won in 2011 to 298 billion won in 2015, attracting attention as a new industry in which insects, which were considered disgusting objects, lay golden eggs.

Accordingly, the use of insects is rapidly expanding to food, pesticide substitutes, and food and drug raw materials.

Among insects, slugs belong to the orders Coleoptera, Scarabaceae, and Asteraceae, which are called “manufacturing” or “slugs” in traditional oriental medicine books such as folk and Donguibogam, and are herbivorous insects with a size of 17 to 24 centimeters, It inhabits Korea, China, Japan, and eastern Siberia, and is known to occur once every 1-2 years from May to October.

Slugs have long been used as oriental medicines for the treatment of liver diseases, etc., and have recently attracted great attention as an insect resource for the search and development of useful bioactive substances. In experiments, the effect of restoring liver lipid metabolism damaged by excessive alcohol intake is known, and its usefulness has been confirmed, such as a study on thrombolytic enzymes and a hepatoprotective effect on hepatotoxicity induced by the administration of carbon tetrachloride in rats. there is a bar

1. Korean Patent Publication No. 10-0353753

An object of the present invention (Disclosure) is to provide a method for preparing and taking a slug powder that can repair liver damaged cells.

Herein, a general summary of the present invention is provided, which should not be construed as limiting the scope of the present invention (This section provides a general summary of the disclosure and is not a comprehensive disclosure of its full scope or all of its features).

In order to solve the above problems, the slug powder manufacturing method according to any one aspect of the various aspects describing the present invention is to wash the mature slugs, and a low-temperature vacuum drying method using a microwave a primary drying step of lowering the water content of the washed slugs to a set initial moisture content; a secondary drying step of drying the slugs provided in the primary drying step at a preset drying temperature and drying time; and a grinding step of pulverizing the slugs provided in the secondary drying step to prepare slug powder.

In the method for producing slug powder according to another aspect of the present invention, the initial moisture content may be 75% to 80%.

In the slug powder manufacturing method according to another aspect of the present invention, the set drying temperature may be 29° C. to 35° C., and the set drying time may be 1 hour to 2 hours.

In the method for producing slug powder according to another aspect of the present invention, the first drying step includes: placing the washed slugs in a vacuum chamber and reducing the pressure; a decompression maintaining step of maintaining the decompression state of the vacuum chamber for a predetermined decompression time; and applying a microwave to the inside of the vacuum chamber after the decompression time has elapsed, and the initial moisture content may be 77%.

In order to solve the above problem, the method for taking slug powder according to any one aspect of the various aspects describing the present invention includes the step of orally administering the slug powder prepared by the method for preparing the slug powder to the user. Thus, the dosing provides the recipient with a total daily dose of 100 to 3,000 mg/kg.B.W. of the slug powder per day.

In the method for taking slug powder according to another aspect of the present invention, the total daily dose may be 100 to 300 mg/kg.B.W.

In the slug powder dosage method according to another aspect of the present invention, the total daily dose may be selectively administered in two or more divided doses.

According to the present invention, by taking slug powder, it is possible to maximize the effect of repairing liver cell damage.

1 is a blood coagulation inhibitory activity of a total of 8 groups.
2 is a histological examination of the liver tissue of the experimental group of a total of 8 groups.

Hereinafter, an embodiment implementing the method for producing and taking the slug powder according to the present invention will be described in detail with reference to the drawings.

However, the intrinsic technical idea of the present invention cannot be said to be limited by the embodiments described below. It is revealed that the range that can be easily suggested as a method of substitution or modification of the embodiments described in is included.

In addition, since the terms used below are selected for convenience of explanation, in grasping the intrinsic technical idea of the present invention, they are not limited to the dictionary meaning and are appropriately interpreted as meanings consistent with the technical spirit of the present invention. it should be

The method for producing slug powder according to the present invention includes a primary drying step, a secondary drying step, and a grinding step.

In the first drying step, the mature slugs are washed, and the moisture content of the washed slugs is lowered to a set initial moisture content by using a low-temperature vacuum drying method using microwaves.

In the second drying step, the slugs provided in the first drying step are dried at a preset drying temperature and drying time.

In the grinding step, the slugs provided in the secondary drying step are pulverized to prepare slug powder.

Table 1 is a table of ingredients of slugs prepared according to the conventional method for preparing slug powder, and Table 2 is a table of ingredients of slugs prepared according to the method for preparing slug powder according to the present invention.

The conventional method for producing slug powder in Table 1 uses a general hot air drying method.

analysis items result unit Test Methods moisture 4.0 g/100g Food standards and specifications, moisture atmospheric pressure drying method Fat 14.8 g/100g Food standards and specifications Crude fat ether extraction method protein 51.8 g/100g Standards and Specifications for Food Crude Protein Protein Analyzer Measurement Method ash 5.6 g/100g Standards and Specifications for Food Batch Test Methods carbohydrate 23.8 g/100g Food standards and standards Carbohydrate calculation method Dietary Fiber 5.8 g/100g Food standards and specifications Total dietary fiber test method

analysis items result unit Test Methods moisture 5.2 g/100g Food standards and specifications, moisture atmospheric pressure drying method Fat 15.2 g/100g Food standards and specifications Crude fat ether extraction method protein 54.7 g/100g Standards and Specifications for Food Crude Protein Protein Analyzer Measurement Method ash 6.2 g/100g Standards and Specifications for Food Batch Test Methods carbohydrate 18.7 g/100g Food standards and standards Carbohydrate calculation method Dietary Fiber 5.2 g/100g Food standards and specifications Total dietary fiber test method

A generally used hot air drying method is drying for 48 hours at a temperature between 45°C and 50°C.

Comparing Tables 1 and 2, the slugs prepared according to the method for preparing slug powder according to the present invention have higher moisture and protein content, compared to slugs prepared according to the conventional method for preparing slug powder.

The method for producing slug powder according to the present invention employs a two-step drying method in which a low-temperature vacuum drying method using microwaves and a hot air drying method are sequentially performed.

Since the microwave directly applies electromagnetic energy to the moisture itself contained in the drying object, the destruction of nutrients is minimized. In addition, if the object to be dried is dried in a vacuum state, it is possible to dry even at a low temperature and to form a uniform drying state from the epidermis to the inside of the object to be dried.

Accordingly, it is possible to dry at a low temperature even in the secondary drying stage, and it is possible to prevent chemical transformation of slugs as much as possible.

In the method for producing slug powder according to the present invention, the initial moisture content is 75% to 80%, preferably 77%.

In addition, in the method for producing slug powder according to the present invention, the set drying temperature is 29°C to 35°C, preferably 30°C to 33°C.

In addition, in the method for producing slug powder according to the present invention, the set drying time is 1 hour to 2 hours, preferably 2 hours 30 minutes.

The primary drying step in the slug powder manufacturing method according to the present invention includes the steps of placing the washed slugs in a vacuum chamber and reducing the pressure, and applying microwaves to the inside of the vacuum chamber when the pressure inside the vacuum chamber is reduced to a set pressure.

In addition, the initial moisture content is preferably 77%.

In general, in the vacuum drying method, since moisture evaporates rapidly, the tissue of the object to be dried is highly likely to be destroyed or deformed.

In the method for producing slug powder according to the present invention, the primary drying step includes the steps of placing the washed slugs in a vacuum chamber and decompressing, maintaining the reduced pressure in the vacuum chamber for a predetermined decompression time, and the lapse of decompression time Then, it may include applying a microwave to the inside of the vacuum chamber.

When the fully ripened and washed slugs are put in a vacuum chamber and reduced pressure, the skin of the slugs as well as the soft slug tissue swells up, and the moisture inside starts to evaporate due to the decrease in pressure.

If the decompression state is maintained for the decompression time, the tissue of the slugs is relaxed without being heated.

After curing under reduced pressure, if microwaves are applied, the moisture inside the slug tissue is heated and rapidly released to the outside.

In this process, the slug's tissue is destroyed or deformed. This tissue modification has the effect of increasing the drying efficiency of the secondary drying step performed thereafter.

In addition, by vacuum drying the primary drying, there is an advantage that the fragrance component inside the slug tissue can be removed.

If the initial moisture content in the primary drying step is high, there is a problem in that the drying time in the secondary drying step is long.

On the other hand, if the initial moisture content is excessively low, the tissue destruction of the slugs is deepened, and there is a problem in that workability after the first drying step is deteriorated.

By achieving the initial moisture content of 75% to 80%, preferably 77%, the slugs are semi-dry and maintain their shape, so that the operation of the secondary drying step can be easily performed.

The method for taking slug powder according to the present invention includes a method of orally administering to a user the slug powder prepared by the above-described method for preparing slug powder.

At this time, the administration provides the recipient with a total daily dose of 100 to 3,000 mg/kg.B.W. slug powder per day.

The total daily dose may be between 100 and 300 mg/kg.B.W.

The total daily dose may optionally be administered in two or more divided doses.

Example - Verification of the in vivo antithrombotic activity of ginseng powder.

Experimental evaluation items

The experiment was conducted with reference to the Food and Drug Administration's functional evaluation guidelines for health functional foods (2015) - 'Can help improve blood circulation'.

experimental group

The experimental group was divided into a total of 8 groups.

A total of 8 experimental groups were: normal diet group (G1), slugs (G2, 3,000 mg/kg B.W.) group, CCl4 administration group (G3), CCl4+ pycno-genol (G4, 500 mg/kg B.W.) group, CCl4+ slugs (G5, 100mg/kg B.W.) group, CCl4+ slug (G6, 300mg/kg B.W.) group, CCl4+slug (G7, 1,000mg/kg B.W.) group, CCl4+ slug (G8, 3,000mg/kg B.W.) group, Table 3 below is shown by comparing the composition and dosage of the experimental group of the above-mentioned 8 groups.

army substance to be administered daily dose
(mg/kg BW) dose amount
(mL/kg) G1: ND non-treat - 10 G2: PBS Slugs (PBS) 3,000 10 G3: CCl ₄ * CCl ₄ * - 10 G4: CCl ₄ +Pycnogenol CCl ₄ +Pycnogenol 500 10 G5: CCl ₄ +PBS-100 CCl ₄ + Slugs (PBS) 100 10 G6: CCl ₄ +PBS-300 CCl ₄ + Slugs (PBS) 300 10 G7: CCl ₄ +PBS-1000 CCl ₄ + Slugs (PBS) 1,000 10 G8: CCl ₄ +PBS-3000 CCl ₄ + Slugs (PBS) 3,000 10

In the G3 experimental group, CCl ₄ was diluted to 25% with olive oil and then administered intraperitoneally at 4 ml/kg BW twice a week.

laboratory animal

Six-week-old SD-male rats weighing about 250 g were purchased from 10 rats in each experimental group (G1 to G8) (Samtaco Co., Ltd., BIO KOREA) and used in the experiment. In order to adapt to the environment, the normal diet group (G1) had 1314 IRR [Altromin Spezialfutter GmbH & Co. KG (Im Seelenkamp 20, D-3 2791 Lage Postfach 11 20, D-32770 Lage_Germany)] was supplied as a basic diet.

In this experiment, to examine the antithrombotic effect of slug powder, pycnogenol as a slug powder or positive control was orally administered 5 times for 5 days before CCl ₄ administration at the same time every day to all animals.

After that, the normal diet group (G1) and carbon tetrachloride (CCl ₄ , Carbontetrachloride) was classified into CCl ₄ group (G3), which is an animal group with abnormal blood coagulation system.

The breeding house used a polycarbonate breeding box, and the temperature and humidity were adjusted to 22±2℃, 55±5%, and the light and dark was maintained at 8:00am-8:00pm, and the indoor lighting was maintained at light/dark every 12 hours.

substance to be administered

100, 300, 1,000, and 3,000 mg/kg bw of slugs + Vit C, slugs or pycnogenol were ingested to the experimental animals for 4 weeks, as shown in Table 1.

test

For laboratory animals, weight gain, autopsy, blood tests, and histopathological tests were performed.

weight gain

Measurements were made once a week during the administration period and on the day of autopsy when the experimental animals were introduced, when the above-mentioned 8 groups were separated.

autopsy

After removing the diet from 12 hours before the day before the experiment, the body weight of the animals was measured on the day of the experiment. Abdominal fat mass was measured by anesthetizing with ether and then excising the abdominal incision to extract both the fat around the epididymis and the fat around the kidney.

blood test

Some of the blood collected was left at room temperature for 30 minutes and then centrifuged at 3,000 rpm for 20 minutes to separate serum and stored at -70°C until used for analysis. Blood in sodium citrate tube was centrifuged at 3,000 rpm for 20 minutes and plasma was stored at -70°C until used for analysis. Triglyceride (TG), Total cholesterol (T-CHO), High density lipiprotein cholesterol (HDL-c), Low density lipiprotein cholesterol (LDL-c), Aspartate transaminase (AST), Alanine transaminase (ALT), Alkaline phosphatase (ALP) , Blood urea nitrogen (BUN), Creatinine (CRE), Total protein (TP), and Albumin (ALB) were analyzed using serum, and an automatic biochemical analyzer (Hitachi 3100, Hitachi Ltd, Japan) was used. Prothrombin time (PT), Activated partial thromboplastin time (aPTT). An automatic coagulation analyzer (CA660, Sysmex Co. Ltd) was used for fibrinogen (Fbg) analysis.

histopathological examination

Histopathological examination: Histopathological examination was performed using liver tissue fixed in formalin. After the fixed tissue was cut to a thickness of about 4 mm, it was made into a paraffin block through general tissue processing and embedding process. After that, sections were cut to a thickness of 4 μm using a rotary microtome, and then Hematoxylin and eosin stain (HE stain) and Masson trichrome stain were performed. While observing the stained slides with an optical microscope, they were graded from 0 to 4 points according to the area of chirosis.

analysis

All experimental results were expressed as the average value and standard error of 8 animals. The statistical method to know the significance of the difference between each group is SPSS 18.0 (Statistical Pack -age for Social Sciences, SPSS Inc., Chicagom IL, USA) software program. Duncan's multiple range test was performed using

result

body weight, liver and kidney tissue weight.

Table 4 below shows the body weight and organ weight of the experimental group of a total of 8 groups. Each value is the mean ± standard deviation, n=8.

According to Table 4, as a result of measuring body weight, liver and kidney tissue weight, no significant change was found in absolute and relative organ weight.

Group Weight (g) Absolute organ weight (g) Relative organ weight (g) Liver Kidney Liver Kidney G1 ND 209.1 ± 9.40 7.0 ± 0.4 1.7 ± 0.1 3.3 ± 0.2 0.8 ± 0.0 G2 PBS 213.4 ± 21.0 7.1 ± 0.6 1.7 ± 0.1 3.4 ± 0.4 0.8 ± 0.0 G3 CCl ₄ * 218.1 ± 8.10 10.7 ± 0.8 1.8 ± 0.2 4.9 ± 0.3 0.8 ± 0.1 G4 CCl ₄ +Pycnogenol 219.0 ± 11.6 11.6 ± 2.4 1.7 ± 0.2 5.3 ± 1.1 0.8 ± 0.1 G5 CCl ₄ +PBS-100 212.4 ± 9.10 8.9 ± 1.9 1.7 ± 0.1 4.2 ± 0.9 0.8 ± 0.1 G6 CCl ₄ +PBS-300 212.8 ± 11.2 10.7 ± 1.2 1.8 ± 0.2 5.1 ± 0.7 0.8 ± 0.1 G7 CCl ₄ +PBS-1000 218.5 ± 9.30 10.3 ± 0.6 1.8 ± 0.2 4.7 ± 0.5 0.8 ± 0.1 G8 CCl ₄ +PBS-3000 211.9 ± 14.1 10.4 ± 0.6 1.6 ± 0.1 4.9 ± 0.4 0.8 ± 0.1

Effect on Hematological Changes

Table 5 below is the biochemical index of the experimental group of a total of 8 groups.

According to Table 5, hematological changes of each experimental group are compared as follows.

- For the entire experimental group (G1 to G8), there was no statistical significance in total protein (TP) and albumin (Alb) according to sample treatment. In the case of TP, the production of TP decreases due to insufficient production in liver disease, and it increases in immunoglobulinosis during liver disease. is considered to be highly probable.

- AST, which is an indicator of liver damage in serum, showed a tendency to increase in both G3~G8 compared to G1 and G2 in the untreated group. In particular, the slug powder 100 mg/kg administration group (G5) significantly decreased by 39.8%, and showed a tendency to increase thereafter.

- In the case of ALT, both G3~G8 showed a tendency to increase compared to the untreated group G1 and G2, but compared with G3, the carbon tetrachloride alone group, all of the sample treated groups (G4~G8) showed a tendency to decrease. The slug powder 300 mg/kg administration group (G6) significantly decreased by about 33.5%, and showed a tendency to increase thereafter.

Group TP (g/dL) Alb (g/dL) AST (IU/L) ALT (IU/L) ALP (IU/L) G1 ND 5.68±0.23 2.46±0.14 172.53±32.68 31.89±5.83 31.89±5.83 G2 PBS 5.46±0.18 2.40±0.09 151.46±31.46 32.64±4.37 32.64±4.37 G3 CCl ₄ * 5.58±0.17 2.45±0.12 3415.63±1494.41 2393.50±907.99 2393.50±907.99 G4 CCl ₄ +Pycnogenol 5.44±0.21 2.33±0.08 3287.29±1865.45 2226.00±904.32 2226.00±904.32 G5 CCl ₄ +PBS-100 5.89±0.50 2.55±0.19 2057.00±574.75 1595.50±504.02 1595.50±504.02 G6 CCl ₄ +PBS-300 5.66±0.35 2.48±0.17 2269.91±1456.11 1155.96±819.99 1155.96±819.99 G7 CCl ₄ +PBS-1000 5.90±0.39 2.54±0.21 2231.38±605.53 1805.88±555.73 1805.88±555.73 G8 CCl ₄ +PBS-3000 5.46±0.18 2.34±0.07 2911.25±957.54 2292.28±1079.04 2292.25±1079.04

- On the other hand, the enzyme used as an indicator to check liver damage caused by carbon tetrachloride (CCl ₄ ) is AST, ALT, alanine transamidase, lactate dehydrogenase, ornitine carbonyl transferase, etc. ALT activity is widely used in clinical trials as an indicator to measure the degree of liver damage.

- The increase in AST and ALT is a phenomenon that occurs in hepatocellular diseases, and ALP is increased due to hepatic refractory obstruction due to tumor, hepatitis, cirrhosis, etc. In this experiment, AST and ALT ALP were significantly increased by administration of carbon tetrachloride. However, in the case of the slug powder administration group, the low concentration (100 mg~300 mg) G5 and G6 showed a significant decrease compared to the carbon tetrachloride single administration group (G3), and thereafter, the high concentration group (G7 ~ G8) showed a tendency to increase again. .

Therefore, it is judged that the mixed powder of slugs in the G5 and G6 groups plays a major role in repairing the damage to the hepatocytes.

Effect on blood clotting inhibition

1 is a blood coagulation inhibitory activity of a total of 8 groups.

- PT and aPTT, which are blood coagulation indicators measured for blood improvement evaluation, are indicators that reflect the extrinsic pathway and the intrinsic pathway of the blood coagulation system, respectively, and the antithrombotic effect can be estimated.

PT, a screening test for the exogenous coagulation system, puts tissue-thromboplastin containing Ca ²⁺ into plasma and measures the coagulation time to measure the concentration and function of plasma coagulation factors II, VII, X and fibrinogen acting on the exogenous coagulation system. used to evaluate

On the other hand, aPTT is an overall screening test for the coagulation mechanism, and is a sensitive test that evaluates the overall function of the intrinsic pathway (coagulation factors II, VII, IX, X, XI, XII).

- As shown in FIG. 1, PT and aPTT, which are blood coagulation-related indicators, were statistically significantly decreased in G5 (100 mg) and G6 (300 mg) compared to the positive control pycnogenol (G4), but 300 mg In the abnormally administered group (G7~G8), there was a tendency to extend again.

However, compared to the control group, the PT of the slug powder addition group showed a tendency to prolong, and the aPTT decreased in the high fat control group compared to the control group, but showed a tendency to increase according to the slug powder addition group.

The prolongation of PT and aPPT is due to the deficiency of blood coagulation factors, which is also related to the activation of liver function.

A significant decrease in PT and aPPT in G5 and G6 can be judged to indicate that liver damage is minimal.

In addition, fibrinogen, a blood coagulation factor, also decreased significantly according to the amount of slugs added, which is considered to be likely to contribute to the improvement of blood circulation disorders by delaying blood coagulation.

Effect on changes in liver tissue

2 is a histological examination of liver tissues of a total of 8 experimental groups.

As shown in FIG. 2 , as a result of performing MT stain and HE stain to confirm the condition of the liver, in the normal group (G1) and in the G2 group administered with 3,000 mg of slugs in normal animals, degeneration of liver lobules and sinusoid structures or central venous strain Satellite changes, specific changes in the bile duct and portal vein were not observed, whereas significant necrosis and fat deposition were observed in the carbon tetrachloride alone group (G3), and bleeding was confirmed in some animals.

In addition, a statistically significant decrease was confirmed in all groups except for 3,000 mg (G8) of the slugs administered group, and also significantly decreased in the pycnogenol administration group (G4), which was used as a positive control. It was confirmed that it can inhibit liver damage induced by

Experiment result

It was confirmed that the method for taking slug powder according to the present invention is effective for improving liver function and repairing liver damage of slug powder.

Claims (7)

A primary drying step of washing the mature slugs and lowering the moisture content of the slugs washed by a low-temperature vacuum drying method using a microwave to a set initial moisture content;
a secondary drying step of drying the slugs provided in the primary drying step at a preset drying temperature and drying time; and
Grinding step of pulverizing the slugs provided in the secondary drying step to produce slug powder;
The set drying temperature is 29 ℃ to 35 ℃,
The set drying time is 1 hour to 2 hours,
The first drying step is
placing the washed slugs in a vacuum chamber and reducing the pressure;
a decompression maintaining step of maintaining the decompression state of the vacuum chamber for a predetermined decompression time;
and applying microwaves to the inside of the vacuum chamber after the decompression time has elapsed;
The initial moisture content is 77% slug powder manufacturing method. delete delete delete delete delete delete

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