KR102406838B1 - Closure of vessel with a sterilizing composition for companion animals containing active biological materials - Google Patents

Abstract

The present invention relates to a sterilizing composition for companion animals including an active biological material that can have high sterilization power without harming companion animals by using an active biological material containing bacteriophage. The present invention provides a bactericidal composition for companion animals comprising an active biological material consisting of an active biological material, an excipient, a thickener, a flavoring agent, a sweetener and a dispersing agent.

Description

Closure of vessel with a sterilizing composition for companion animals containing active biological materials

The present invention relates to a sterilizing composition for companion animals containing an active biological material, and more particularly, it contains an active biological material that can have high sterilization power without harming companion animals by using an active biological material containing bacteriophages. It relates to a sterilizing composition for companion animals.

Bacteriophage is a kind of virus that infects bacteria, and is often called phage for short. Bacteriophage is an organism with a simple structure in which a protein coat surrounds the center of a genetic material composed of nucleic acids, and nucleic acids are composed of single-stranded or double-stranded DNA or RNA. Bacteriophages require a host for survival, and it is known that certain bacteriophages exist in all bacteria. Bacteriophage penetrates into the host, completes the replication process, and then expresses a group of enzymes necessary to decompose the cell wall of the host bacteria. These enzymes disrupt the cell wall by attacking the peptidoglycan layer of the cell wall, which is responsible for the rigidity and mechanical strength of the cell wall.

These bacteriophages were discovered in 1915 by British bacteriologist Twort in a study on the phenomenon of transparent dissolution of Staphylococcus aureus (Micrococcus) colonies by something. In addition, in 1917, French bacteriologist d'Herelle discovered that there was something with the action of dissolving Shigella disentriae in the filtrate of the stool of a patient with this disease. Named as a bacteriophage from the meaning. Since then, bacteriophages for various pathogens such as Shigella, Typhoid, and Cholera have been continuously discovered. However, after penicillin was discovered by Flemming in 1950, research on bacteriophages continued only in some Eastern European countries due to the widespread use of antibiotics, and in other regions, it was somewhat withered. However, since 2000, due to the misuse of antibiotics, the frequency of appearance of multidrug-resistant pathogenic bacteria has increased, and many problems with existing antibiotics have been highlighted. It has received a lot of attention centering on them and is being actively carried out again.

However, bacteriophages have been mainly used for the purpose of alleviating or treating specific infectious diseases by oral administration or injection to the human body. However, in the case of bacteriophage, when activated by contact with water, it is known that the shelf life is very short, and the existing methods have limitations in being used in many parts due to the complicated administration method.

(0001) Republic of Korea Patent No. 10-0602418 (0002) Republic of Korea Patent No. 10-1478365

In order to solve the above problem, the present invention is to provide a sterilizing composition for companion animals including an active biological material that can have high sterilization power without harming the companion animal by using the active biological material containing bacteriophages.

In order to solve the above problems, the present invention provides a sterilizing composition for companion animals comprising an active biological material consisting of an active biological material, an excipient, a thickener, a flavoring agent, a sweetener and a dispersing agent.

In one embodiment, the active biological material may be a bacteriophage.

In one embodiment, the sterilizing composition may be in the form of a dry powder.

In one embodiment, the bactericidal composition may further include a non-pathogenic microorganism.

The present invention also includes (a) an outer cap portion to which the container is coupled to the inner circumferential surface; and (b) a body part located inside the outer cap part and having an accommodation space in which an active biological material, excipients, thickeners, flavoring agents, sweeteners and dispersants are accommodated in a sterilizing composition for companion animals. Inside the body portion (i) a pressing member formed on the upper side of the accommodating space; and (ii) a spherical elastic body located below the receiving space and sealing the receiving space, wherein the pressing member presses the elastic body by pressing to release the elastic body, thereby opening the receiving space. It provides a container closure comprising a sterilizing composition for animals.

In one embodiment, the lower portion of the main body portion may be formed with a fall preventing portion for preventing the detached elastic body from falling.

In one embodiment, the inner circumferential surface of the main body may be formed with a forced-fit sealing that can fix the spherical elastic body at a predetermined position and prevent the liquid inside the container from penetrating into the receiving space.

The sterilization composition for companion animals according to the present invention can be sterilized without the use of antibiotics, and by using bacteriophages that have antibacterial properties selectively for specific pathogens, the effect on companion animals can be minimized even when companion animals drink. It can be used for purposes such as sterilizing drinking water for companion animals and assisting with antibiotic effects.

1 is a perspective view of a state in which a container cap according to an embodiment of the present invention is installed.
2 is a cross-sectional view of a container stopper according to an embodiment of the present invention, (a) is an overall cross-sectional view (b) is an enlarged view of the sealing portion of the forced fitting.
Figure 3 shows a cross-sectional view of a container stopper according to an embodiment of the present invention, (a) is an overall cross-sectional view (b) is an enlarged view of the sealing portion of the forced fitting.
4 shows a method of operating a container closure according to an embodiment of the present invention, (a) is a state before operation, (b) is an elastic body due to the pressurization of the pressing member, showing that the active biological material is falling will be.
5 is a cross-sectional view of a container stopper having a fall prevention part according to an embodiment of the present invention. (a) is an overall cross-sectional view, (b) is a view showing that the elastic body is detached due to the pressing of the pressing member and is seated in the fall prevention part; , (c) shows a perspective view of the fall prevention part.
6 is a view showing an elastic body and a cross-section thereof according to an embodiment of the present invention. (a) is an external appearance of the elastic body, (b) is an elastic body having a hollow part, (c) is an elastic body including a polymer resin or a metal core part therein are shown respectively.
7 is an enlarged view of the pressing means according to an embodiment of the present invention, (a) a cross-sectional view before applying pressure to the pressing means, (b) is a cross-sectional view after the perforating means perforates the elastic body by applying pressure to the pressing means indicates
8 is a view showing a method for assembling a container closure according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail. In describing the present invention, if it is determined that a detailed description of a related known technology may obscure the gist of the present invention, the detailed description thereof will be omitted. Throughout the specification, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

Since the present invention can apply various transformations and can have various embodiments, specific embodiments are illustrated and described in detail in the detailed description. However, this is not intended to limit the present invention to specific embodiments, it should be understood to include all modifications, equivalents and substitutes included in the spirit and scope of the present invention.

The technology disclosed herein is not limited to the implementations described herein and may be embodied in other forms. However, the embodiments introduced herein are provided so that the disclosed content may be thorough and complete, and the technical spirit of the present technology may be sufficiently conveyed to those skilled in the art. In order to clearly express the components of each device in the drawings, the sizes such as widths and thicknesses of the components are slightly enlarged. In the description of the drawings as a whole, it has been described from an observer's point of view, and when an element is referred to as being positioned on another element, this means that the element may be positioned directly on the other element or an additional element may be interposed between the elements. include In addition, those of ordinary skill in the art will be able to implement the spirit of the present invention in various other forms without departing from the technical spirit of the present invention. And the same reference numerals in the plurality of drawings refer to elements that are substantially the same as each other.

Terms used in the present invention are only used to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise. In the present invention, the terms include or have is intended to designate that a feature, number, step, operation, component, part, or combination thereof described in the specification exists, but one or more other features, number, step , it should be understood that it does not preclude in advance the possibility of the presence or addition of an operation, component, part, or combination thereof.

On the other hand, the meaning of the terms described in this specification should be understood as follows. Terms such as “first” or “second” are for distinguishing one component from other components, and the scope of rights should not be limited by these terms. For example, a first component may be termed a second component, and similarly, a second component may also be termed a first component.

In addition, the singular expression should be understood to include the plural expression unless the context clearly dictates otherwise, and terms such as “comprise” or “have” refer to the described feature, number, step, operation, component, or part. It is to be understood that this is intended to indicate that there is a combination thereof, but does not preclude the possibility of addition or existence of one or more other features or numbers, steps, operations, components, parts, or combinations thereof. In addition, in performing the method or the manufacturing method, each process constituting the method may occur differently from the specified order unless a specific order is clearly described in context. That is, each process may occur in the same order as specified, may be performed substantially simultaneously, or may be performed in the reverse order.

In this specification, the term 'and/or' includes a combination of a plurality of listed items or any of a plurality of listed items. In this specification, 'A or B' may include 'A', 'B', or 'both A and B'.

The present invention relates to a bactericidal composition for companion animals comprising an active biological material comprising an active biological material, an excipient, a thickener, a flavoring agent, a sweetener and a dispersing agent.

The active biological material means a biological material capable of killing pathogens as it has activity against pathogens, and any biological material capable of killing pathogens can be used without limitation, but preferably bacteriophages.

Since the bacteriophage acts specifically on each pathogen, it is preferable to select and use an appropriate bacteriophage according to the type of each pathogen. As an example, bacteriophages for sterilizing Salmonella may use a slurry of Salmonella bacteriophages, such as an Intralytix product on the market under the name of "SalmoFresh".

In addition, as a bacteriophage for sterilizing bacteria belonging to the Bacillus genus, Caudovirales, Microviridae, Leviviridae, Inoviridae, Bacteriophages of the family Tectiviridae, or the family Corticoviridae can be used.

SK311,

812, 06, 40, 58, 119, 130, 131, 200, 1623 등. 시포비리대 과(Siphoviridae family)에 속하는 박테리오파지로서는 박테리오파지 AC1, AC2, AC3, A6"C", A7, A8, A9"C", A10, b581, b595n, B3, B33, B39, BI-1, C22, CA-1, CA-2, CA-3, CA-4, CA-5, D, D3, D11, D37, D40, D62, D3112, F7, F10, g, gd, ge, gf, HK2, Hw12, Jb19, KF1, L39X35, L54a, M42, N1, N2, N3, N4, N5, N7, N8, N9, N10, N11, N12, N13, N14, N15, N16, OXN-32P, O6N-52P, P52, P87, Ph6, Ph12, Ph14, PCH-1, PC13-1, PC35-1, PH2, PH51, PH93, PH132, PMW, PM13, PM57, PM61, PM62, PM63, PM69, PM105, PM113, PM681, PM682, PO4, PP1, PP4, PP5, PP64, PP65, PP66, PP71, PP86, PP88, PP92, PP401, PP711, PP891, Pssy41, Pssy42, Pssy403, Pssy404, Pssy420, Pssy923, PS4, PS-10, Pz, S1, S6, SD1, SL1, SL3, SL5, SM, UC-18, U4, U15, S1, S2, S3, S4, S5, Z1, Z4, X2,

C5,

C11,

C11-1,

C13,

C15,

MO,

X,

04,

11,

240,

B5-2,

D,

RE, ω, 2, 2F, 3A, 3B, 3C, 5, 6, 7, 7m, 11, 13, 13/441, 14, 15, 16, 20, 21, 24, 28, 28A, 29, 31, 31B, 37, 40, 42B, 42C, 42D, 42E, 44, 44A, 45, 47, 47A, 47C, 48, 49, 51, 52, 52A, 52B, 53, 54, 54X1, 55, 61, 69, 70, 71, 71A, 72, 73, 75, 76, 77, 78, 79, 80, 80α, 81, 82, 82A, 83A, 84, 85, 86, 88, 88A, 89, 90, 92, 93, 94, 95, 96, 101, 102, 105, 107, 108, 110, 111, 115, 129/16, 129-26, 130, 130A, 148, 155, 157, 157A, 160, 165, 174, 187, 198, 218, 222, 236, 242, 246, 249, 258, 269, 275, 275A, 275B, 295, 297, 309, 318, 342, 350, 351, 356, 357-1, 400-1, 456, 459, 471, 471A, 489, 581, 594n, 676, 898, 1139, 1154A, 1259, 1314, 1363/14, 1380, 1405, 1563, 2148, 2460, 2638A, 2638B, 2638C, 2731, 2792A, 2792B, 2818, 2835, 2848A, 3619, 5841, 12100 등, 포도비리대 과(Podoviridae family)에 속하는 박테리오파지로서는 박테리오파지 A856, B26, CI-1, CI-2, C5, D, gh-1, F116, HF, H90, K5, K6, K104, K109, K166, K267, N4, N5, O6N-25P, PE69, Pf, PPN25, PPN35, PPN89, PPN91, PP2, PP3, PP4, PP6, PP7, PP8, PP56, PP87, PP114, PP206, PP207, PP306, PP651, Psp231a, Pssy401, Pssy9220, ps1, PTB2, PTB20, PTB42, PX1, PX3, PX10, PX12, PX14, PYO70, PYO71, R, SH6, SH133, tf, Ya5, Ya7,

BS,

Kf77,

-MC,

mnF82,

PLS27,

PLS743,

S-1, 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 12B, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 31, 53, 73, 119X, 145, 147, 170, 267, 284, 308, 525 등이 있다.. 이에 더하여 활용 가능한 박테리오파지로는 American Type Culture Collection에서 다음의 접근 번호(accession number)로 구입하여 사용할 수 있는 박테리오파지들도 있다. ATCC 12055-B1, ATCC 12055-B2, ATCC 12055-B3, ATCC 14205-B1, ATCC 14206-B1, ATCC 14207-B1 , ATCC 14209-B1, ATCC 14210-B1, ATCC 14211-B1, ATCC 14212-B1, ATCC 14213-B1, ATCC 14214-B1, ATCC 15692-B2, ATCC 15692-B3, ATCC 25102-B1, ATCC BAA-26-B1, ATCC BAA-27-B1, ATCC BAA-28-B1, ATCC BAA-28-B2, ATCC BAA-29-B1, ATCC BAA-30-B1, ATCC BAA-31-B1, ATCC BAA-47-B1, ATCC BAA-79-B1, ATCC BAA-81-B1, ATCC BAA-81-B2 등이 그 예이다.In addition, bacteriophages that are effective against pathogens can be used without limitation. The bacteriophages that can be utilized in the present invention are presented as follows. As bacteriophages belonging to the Myoviridae family, bacteriophages A, EW, K, Ph5, Ph9, Ph10, Ph13, P1, P2, P3, P4, P8, P9, P10, RG, SB-1, S3K, Twort ,

SK311,

812, 06, 40, 58, 119, 130, 131, 200, 1623, etc. As a bacteriophage belonging to the Siphoviridae family, the bacteriophages AC1, AC2, AC3, A6"C", A7, A8, A9"C", A10, b581, b595n, B3, B33, B39, BI-1, C22 , CA-1, CA-2, CA-3, CA-4, CA-5, D, D3, D11, D37, D40, D62, D3112, F7, F10, g, gd, ge, gf, HK2, Hw12 , Jb19, KF1, L39X35, L54a, M42, N1, N2, N3, N4, N5, N7, N8, N9, N10, N11, N12, N13, N14, N15, N16, OXN-32P, O6N-52P, P52 , P87, Ph6, Ph12, Ph14, PCH-1, PC13-1, PC35-1, PH2, PH51, PH93, PH132, PMW, PM13, PM57, PM61, PM62, PM63, PM69, PM105, PM113, PM681, PM682 , PO4, PP1, PP4, PP5, PP64, PP65, PP66, PP71, PP86, PP88, PP92, PP401, PP711, PP891, Pssy41, Pssy42, Pssy403, Pssy404, Pssy420, Pssy923, PS4, PS-10, Pz, S1 , S6, SD1, SL1, SL3, SL5, SM, UC-18, U4, U15, S1, S2, S3, S4, S5, Z1, Z4, X2,

C5,

C11,

C11-1,

C13,

C15,

MO,

X,

04,

11,

240,

B5-2,

D,

RE, ω, 2, 2F, 3A, 3B, 3C, 5, 6, 7, 7m, 11, 13, 13/441, 14, 15, 16, 20, 21, 24, 28, 28A, 29, 31, 31B, 37, 40, 42B, 42C, 42D, 42E, 44, 44A, 45, 47, 47A, 47C, 48, 49, 51, 52, 52A, 52B, 53, 54, 54X1, 55, 61, 69, 70, 71, 71A, 72, 73, 75, 76, 77, 78, 79, 80, 80α, 81, 82, 82A, 83A, 84, 85, 86, 88, 88A, 89, 90, 92, 93, 94, 95, 96, 101, 102, 105, 107, 108, 110, 111, 115, 129/16, 129-26, 130, 130A, 148, 155, 157, 157A, 160, 165, 174, 187, 198, 218, 222, 236, 242, 246, 249, 258, 269, 275, 275A, 275B, 295, 297, 309, 318, 342, 350, 351, 356, 357-1, 400-1, 456, 459, 471, 471A, 489, 581, 594n, 676, 898, 1139, 1154A, 1259, 1314, 1363/14, 1380, 1405, 1563, 2148, 2460, 2638A, 2638B, 2638C, 2731, 2792A, 2792B, 2818, 2835, 2848A, 3619, 5841, 12100, etc., as a bacteriophage belonging to the Podoviridae family, as a bacteriophage A856, B26, CI-1, CI-2, C5, D, gh-1, F116, HF, H90, K5, K6, K104, K109, K166, K267, N4, N5, O6N-25P, PE69, Pf, PPN25, PPN35, PPN89, PPN91, PP2, PP3, PP4, PP6, PP7, PP8, PP56, PP87, PP114, PP206, PP207, PP306, P P651, Psp231a, Pssy401, Pssy9220, ps1, PTB2, PTB20, PTB42, PX1, PX3, PX10, PX12, PX14, PYO70, PYO71, R, SH6, SH133, tf, Ya5, Ya7,

BS,

Kf77,

-MC,

mnF82,

PLS27,

PLS743,

S-1, 1, 2, 3, 4, 5, 6, 8, 9, 10, 11, 12, 12B, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 31, 53, 73, 119X, 145, 147, 170, 267, 284, 308, 525, etc. There are also bacteriophages that can be purchased and used. ATCC 12055-B1, ATCC 12055-B2, ATCC 12055-B3, ATCC 14205-B1, ATCC 14206-B1, ATCC 14207-B1, ATCC 14209-B1, ATCC 14210-B1, ATCC 14211-B1, ATCC 14212-B1, ATCC 14213-B1, ATCC 14214-B1, ATCC 15692-B2, ATCC 15692-B3, ATCC 25102-B1, ATCC BAA-26-B1, ATCC BAA-27-B1, ATCC BAA-28-B1, ATCC BAA-28 -B2, ATCC BAA-29-B1, ATCC BAA-30-B1, ATCC BAA-31-B1, ATCC BAA-47-B1, ATCC BAA-79-B1, ATCC BAA-81-B1, ATCC BAA-81- B2 is an example.

Pathogenic bacteria that can be applied by the composition containing the bacteriophage as an active ingredient in the present invention include all pathogenic bacteria having a cell wall. Representative examples include Staphylococcus aureus, Salmonella enteritidis, and Enterococcus faecalis, but this is only representatively selected because it cannot show all examples of application to all pathogenic bacteria, and Enterobacteriacae , Staphylococcus genus, Enterococcus, Streptococcus, Staphylococcus epidermidis, Coagulase-negative Staphylococci) Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Enterococcus faecalis, Enterococcus stuartii, Providencia stuartii ), Proteus mirabilis, Morganella morganii, Acinetobacter calcoaceticus, Enterobacter aerogenes, Streptococcus agalactiae ), Streptococcus avium, Streptococcus bovis, Streptococcus durans, Streptococcus faecalis, Streptococcus pneumoniae, Streptococcus pneumoniae Ness (Streptococcus pyogenes), Streptococcus suis (Streptococcus suis), Streptococcus viridans (Streptococcus viridans), Streptococcus salivariu Streptococcus salivarius, Burkholderia cepacia, Stenotrophomonas maltophilia, Acaligenes xylosoxidans, non-tuberculous mycobacteria, nontuberculous mycobacteria Mycobacterium bovis, Mycobacterium smegmatis, Mycobacterium tuberculosis, Burkholderia multivorans, Burkholderia star Vilis (Burkholderia stablis), Burkholderia vietnamesis (Burkholderia vietnamesis) and the like may be the target pathogenic bacteria of the present invention, but is also not limited thereto.

In addition, the sterilization composition using the active biological material as an active ingredient may be provided as a liquid or solid composition, and is preferably prepared as a powder or granules for long-term storage and smooth fall from the container cap to the inside of the container.

The solid composition may be achieved through various drying processes commonly used in the art, and preferably a freeze-drying method may be used. In particular, in the case of the present invention, it is preferable to prepare the freeze-dried granules using the same method as shown in Korean Patent Application Laid-Open No. 2012-0096347.

In addition, the bacteriophage freeze-dried granules prepared as described above are preferably mixed with excipients, thickeners, flavoring agents, sweetening agents and dispersing agents to prepare a powdery formulation.

The excipient is used as a certain extender to increase the insufficient amount of the bacteriophage freeze-dried granules, and the extender that does not affect the bacteriophage freeze-dried granules can be used without limitation. As the excipient, starch, sugar, lactose or mannitol may be used, and more preferably, sugar or starch may be used.

The thickener is added to increase the particles of the powder formulation (granulation) and reduce agglomeration when added to a liquid so that it can be easily mixed. Guar gum, xanthan gum, gum arabic, pectin, cellulose, starch or Agar may be used.

The flavoring agent and sweetener are added to increase the flavor and taste after mixing with the liquid, and can be used in combination with a fruit flavor, meat flavor, herb or natural flavor preferred by companion animals. Separately, meat, fish or algae It can be used by mixing dry powder or extract of dried powder, nutrients, minerals, etc. as a sweetening agent.

The dispersing agent is used to easily mix without clumping when the powdered formulation is mixed with water, and it is preferable to use an edible dispersing agent that does not harm companion animals. In particular, it is more preferable to use a sodium phosphate-based dispersant having a high dispersing power while being used for food.

The bactericidal composition may further include a non-pathogenic microorganism. The main sterilizing component included in the sterilization composition is a bacteriophage, and as described above, one type of bacteriophage is known to have an effect only on one type of pathogenic bacteria. Therefore, it is preferable to mix the powdered formulation with a non-pathogenic microorganism having a beneficial effect on the companion animal and drink it to the companion animal. As the non-pathogenic microorganism, various microorganisms may be selected and used according to the type of companion, but preferably, easily available lactic acid bacteria may be used.

The content of the bacteriophage freeze-dried granules can be freely selected and there is no particular limitation thereto.

In addition, the powder formulation may contain only one type of effective bacteriophage or may contain two or more types of effective bacteriophage. A composition containing only one type of bacteriophage is a suitable composition to reduce and remove only one bacteria, and a composition containing two or more bacteriophages is a suitable composition to reduce and remove two or more bacteria. In actual field application, treatment for various bacteria is required, so if you use a composition containing only one type of bacteriophage, you will have to use several types of compositions that are different from each other in the type of bacteriophage included, If you use a composition containing a type of bacteriophage, the type of composition to be used together is reduced by that much. How to configure the type of bacteriophage included in the composition is only a matter of choice in the application of the present invention.

For reference, it is good to prepare a composition containing as many bacteriophages as possible in the form of a cocktail for practical application. However, if too many types of bacteriophages are included, the number of each bacteriophage is insufficient and a lot of time is required for processing. It is preferable to prepare a mixture containing 2 to 10 types of bacteriophages in advance and then select and use them for each purpose.

The present invention also includes (a) an outer cap portion to which the container is coupled to the inner circumferential surface; and (b) a body part located inside the outer cap part and having an accommodation space in which an active biological material, excipients, thickeners, flavoring agents, sweeteners and dispersants are accommodated in a sterilizing composition for companion animals. Inside the body portion (i) a pressing member formed on the upper side of the accommodating space; and (ii) a spherical elastic body located below the receiving space and sealing the receiving space, wherein the pressing member presses the elastic body by pressing to release the elastic body, thereby opening the receiving space. It provides a container closure comprising a sterilizing composition for animals.

The container stopper may be manufactured so that a commercially available drinking water bottle (container, 400) is coupled to the inner peripheral surface of the outer cap part 100. Generally, commercially available drinking water or beverage bottle 400 has a diameter of 25 mm or 28 mm. In particular, 250 to 500 drinking water bottles, which are easy to mix with the sterilizing composition 240 for companion animals, have an inlet diameter of 25 mm, so that the outer cap part 100 can be combined with the inlet of these drinking water or beverage bottles. It is preferable to be manufactured.

In addition, it is preferable that the outer cap part 100 is coupled to the container 400 by fitting or rotational coupling (see FIG. 1 ). In the case of the drinking water bottle, since the lid is manufactured to be rotationally coupled, it is preferable to be manufactured to be rotationally coupled in the same way as a commercially available lid. It can be manufactured to be fitted.

Therefore, it is preferable that the container stopper including the outer cap part 100 is manufactured so as to be combined with the container in the same standard as the existing drinking water or beverage cover.

The body part 200 is located inside the outer cap part 100, and may be manufactured to have a accommodating space 230 capable of accommodating the sterilizing composition 240 for companion animals therein.

Looking at this in detail, the main body 200 includes a wall formed concentrically with the outer cap part (!00) inside the outer cap part 100, and the upper part of the wall surface, that is, the upper part of the main body part, is a pressing member. It may be manufactured to be sealed through 210 . In this case, the pressing member 210 is preferably formed integrally with the wall portion, and when manufactured and attached as a separate part, it is preferable that the sterilizing composition 240 for companion animals is not leaked to the outside. In addition, the lower portion of the wall is sealed by a spherical elastic body 300 to be described later. That is, the main body 200 is sealed by the upper portion of the circular wall surface by the pressing member 210 and the lower portion by the spherical elastic body 300 to constitute the accommodation space 230 (see FIG. 1 ).

The pressing member 210 is installed on the upper portion of the main body 200, and serves to press and release the elastic body 300, which will be described later, as it changes shape by pressing. The pressing member 300 is preferably formed to be convex upward, and it is preferable to press the elastic body 300 to be released by pressing the elastic body 300 while being concavely deformed downward by the user's pressure (refer to FIG. 4). At this time, the pressing is preferably performed using the user's finger. However, if the pressing member 210 is not smoothly pressed using a finger due to the small area of the pressing member 210, an extension member is connected to the upper portion to press the extension member with the palm of the hand. It is also possible to pressurize in this way. In addition, it is preferable that the pressing member 210 is manufactured not to protrude to the outside of the main body 200 in order to prevent unwanted pressing (see FIG. 1 ). If the pressing member is manufactured to protrude to the outside, pressurization may occur due to its own weight during packaging or loading, and this may cause the elastic body to be pressurized to generate a gap or to deviate from the position so that the active biological material can be mixed into the container. have. Therefore, it is preferable that the pressing member 210 is manufactured so as not to protrude out of the main body 200 in order to prevent such unwanted pressing.

In addition, in order to further protect the pressing member 210 manufactured so as not to protrude out of the main body as described above, and to prevent contamination of the depressed body 200 due to the pressing member 210, the upper portion of the pressing member is protected. A member 500 may be attached. The protective member can be used by simply attaching a film, but it is also possible to prevent pressurization and at the same time prevent intrusion of contaminants by inserting a cap made in a cylindrical shape on the upper portion of the pressing member.

The elastic body 300 is in close contact with the inside of the main body to seal the receiving space, and is preferably manufactured in a spherical shape and inserted into the main body. In addition, the elastic body 300 is manufactured to have a certain elasticity to seal the accommodation space 230 formed in the body part 200 and at the same time to apply a certain pressure by the pressing means 210 to the body part 200 . ) may be installed to be able to escape (see FIG. 4). To this end, the elastic body 300 may be made of rubber, which is a material having elasticity, and more preferably, it may be made of silicone rubber that is harmless to the human body. In addition, the elastic body 300 may be manufactured in a hollow hollow type to have appropriate elasticity, and may be manufactured to have appropriate elasticity by pressing or decompressing air in the hollow part 310 . Conversely, the elastic body 300 may be manufactured so that the specific gravity exceeds 1 to make drinking easier after the fall of the elastic body 300, and in the case of silicone, as the specific gravity is less than 1, the polymer resin inside the elastic body Alternatively, by installing the core part 320 made of metal, the specific gravity of the elastic body 300 may be 1 or more (refer to FIG. 6 ). When the specific gravity of the elastic body exceeds 1, it may fall and then sink into water, and thus does not float when drinking, so that drinking can be made comfortably.

In addition, the elastic body 300 may open the accommodation space 230 by using a method other than falling due to separation as described above. When the elastic body is manufactured to have a hollow structure (FIG. 6 (b)), when the pressurized air inside the elastic body is removed, the volume of the elastic body may be reduced like a balloon. When the volume of the elastic body is reduced in this way, it is possible to drop the sterilizing composition 240 for companion animals through the gap between the elastic body 300 and the main body 200 . For this purpose, a perforating means 260 may be installed on the lower surface of the pressing member 210 , that is, a portion adjacent to the elastic body, so that the elastic body 300 can be punctured (see FIG. 7 ). In addition, barbs or hooks are installed in the perforating means 260 to prevent the elastic body 300 from falling after the perforation, so that the elastic body 300 is suspended from the perforating means 260 after perforation (FIG. 7) Reference).

In addition, a forced fitting seal 220 may be formed on the inner circumferential surface of the main body 200 so that the elastic body 300 can be fixed at a predetermined position. The force-fit seal 220 refers to a circular protrusion formed on the inner circumferential surface of the main body, and the force-fit seal 220 digs in while pressing the elastic body 300 to prevent separation of the elastic body due to impact. Sealability can be improved (refer to (b) of FIG. 2). In addition, the above-mentioned effect can be exerted even if only one of the forced fitting seals is installed (FIG. 3), but it is preferable to install two for the convenience of assembly (FIG. 2). When two of the force-fitting seals are installed, the maximum diameter of the elastic body 300 is positioned between the force-fitting seals 220, so that it can be fitted and assembled at an accurate position, as well as between the force-fitting seals 220. When the spacing of the slits is appropriately adjusted, the forced-fit seal 220 can be compressed from the top and bottom of the maximum diameter portion of the elastic body, so that it can have a higher sealing property (see FIG. 2 ).

A drop prevention unit 250 may be installed at a lower portion of the main body 200 (see FIG. 5 ). The drop prevention unit 250 is installed to prevent the detached elastic body 300 from falling, and it is preferable to have a grid structure to prevent the elastic body 300 from falling and to facilitate the discharge of the active biological material. do (FIG. 5 (c)). That is, when the fall prevention part 250 having a lattice structure as described above is installed, it is possible to prevent the elastic body 300 from falling when the elastic body 300 is detached, as well as for companion animals located in the upper part of the elastic body 300 . The sterilizing composition 240 may be discharged through the side and/or lower surface of the fall prevention part 250 so that it can be mixed smoothly.

Hereinafter, the operation method of the container stopper will be described in detail through the method of using the container stopper of the present invention.

After purchasing the container 400 for drinking water or beverages, remove the original container lid and install the container stopper of the present invention. Alternatively, as a container closure, a product with the container closure of the present invention may be purchased and used.

When the container stopper is installed as described above, the user presses the pressing member 210 to mix the sterilizing composition 240 for companion animals inside. At this time, after removing the protective means 500 made of a film or polymer material installed to protect the pressing member, the pressing member 210 is pressed.

The pressing member 210 is changed in shape by the pressing as described above. Specifically, the pressing member 210 manufactured in an upwardly convex shape is changed to a downwardly concave shape by the pressing as described above (FIG. 4). Reference).

As the shape of the pressing member 210 changes as described above, the elastic body 300 located therein is pressed, and at this time, the elastic body 300 deviates from its original position according to one of the following three methods. The accommodation space 230 is opened.

1. Full drop method

When the elastic body 300 is manufactured, a core part made of a polymer resin or metal is installed therein so that the specific gravity is higher than that of water (FIG. 6(c)). The elastic body 300 manufactured as described above is separated by pressurization, falls to the lower portion of the container 400 and sinks into the drinking water or beverage. In addition, as described above, while the elastic body 300 is falling, the sterilizing composition 240 for companion animals loaded on the upper portion of the elastic body falls simultaneously with the elastic body 300 and is mixed with drinking water or beverages.

2. Use of fall prevention part

When the elastic body 300 is manufactured, a hollow part is formed therein, and then air is injected into the hollow part at a predetermined pressure (FIG. 6(b)). The elastic body 300 manufactured as described above falls to the lower portion by the pressure of the pressing means 210, and falls to the fall prevention unit 250 installed in the lower portion of the main body. At this time, as the fall prevention part 250 is manufactured in a grid shape, the elastic body 300 cannot fall any more and is located inside the fall prevention part 250 (FIG. 5 (b)). While the sterilizing composition 240 for companion animals loaded on the upper portion of the elastic body 300 falls simultaneously with the elastic body 300 . The moment the elastic body is caught in the fall prevention part 250, it moves downward along the surface of the elastic body 300, and since the side and/or lower surface of the fall prevention part 250 are made in a grid shape, the fall prevention part ( 250) through the side and / or lower surface is mixed with drinking water or beverages.

3. Use of means of perforation

When the elastic body is manufactured, a hollow part is formed inside, and then air is injected into the hollow part at a constant pressure (FIG. 6(b)). When the pressing means 210 is pressed, the perforating means 260 installed under the pressing means 210 perforates the elastic body 300 (see FIG. 7), and the perforated elastic body is the perforating means ( It is fixed to the lower part of the pressing means 210 by being caught on the barb or hook installed in the 260). In addition, the volume of the perforated elastic body as described above is reduced as the internal pressure decreases, and is for companion animals loaded on the upper portion of the elastic body 300 through a gap between the main body 200 and the elastic body 300 . The sterilizing composition 240 may fall downward and be mixed with drinking water or beverages.

After mixing the active biological material with drinking water or drinking water according to one of the three methods, the container stopper is removed and drinking is performed.

Hereinafter, the present invention will be described in detail through a method for manufacturing a container closure.

The outer cap portion, the body portion, the pressing member and the fall prevention portion of the container cap may be manufactured through a single mold. The outer cap part, the body part, the pressing member and the fall prevention part can be manufactured in a shape that can be manufactured through the existing polymer resin lid manufacturing facility, and it is preferable to be made of polyethylene, which is the same material as that of the existing lid (Fig. 8(a)).

After turning over the lid manufactured as described above, the sterilizing composition for companion animals is injected into the portion where the pressing member is located. At this time, since the pressing member is manufactured integrally with the main body, the sterilizing composition for companion animals can be loaded on the lower portion of the pressing member (FIG. 8(b)).

After loading the above antiseptic composition for companion animals. The accommodating space is sealed using a spherical elastic body. As described above, it is preferable to install two forced-fitting seals to position the maximum diameter portion of the elastic body between the forced-fitting seals (FIG. 8(c) )).

When it is sealed using the elastic body as described above, and then turned over and combined with the container, the sterilizing composition for companion animals is located in the receiving space formed on the upper part of the elastic body and sealed at the same time (FIG. 8 (d)) .

As the specific parts of the present invention have been described in detail above, for those of ordinary skill in the art, it is clear that these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby. will be. Accordingly, it is intended that the substantial scope of the present invention be defined by the appended claims and their equivalents.

100: outer cap portion 200: body portion
210: pressure member 220: forced fitting sealing
230: accommodation space 240: sterilization composition for companion animals
250: fall prevention part 300: elastic body
400: container 500: protection member

Claims (7)

delete delete delete delete (a) an outer cap portion to which the container is coupled to the inner circumferential surface; and
(b) located inside the outer cap part, the body part having a bacteriophage, excipient, thickener, flavoring agent, sweetener, dispersing agent and a sterilizing composition for companion animals in the form of a dry powder consisting of a non-pathogenic microorganism therein is accommodated in a receiving space ;
including,
Inside the body part
(i) a pressing member formed on the upper side of the accommodation space; and
(ii) a spherical elastic body located below the accommodation space and sealing the accommodation space;
includes,
The pressing member presses the elastic body by pressing to release the elastic body to open the receiving space,
A fall prevention part for preventing the detached elastic body from falling is formed in the lower part of the main body part,
The elastic body separated by the pressing member is located inside the fall prevention part and is separated from the container together with the container in the process of removing the container stopper.
delete 6. The method of claim 5,
A container closure, characterized in that a forced fitting sealing is formed on the inner circumferential surface of the main body to fix the spherical elastic body at a predetermined position and prevent the liquid inside the container from penetrating into the accommodation space.

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