KR102399501B1 - Fermented feedstuffs of seashells using lactic acid bacteria and their preparation method - Google Patents

Abstract

The present invention relates to a shell fermented feed using lactic acid bacteria and a method for manufacturing the same, and to provide a shell fermented feed using lactic acid bacteria comprising a fermented shell lactic acid bacteria as an active ingredient. According to the present invention, it is possible to improve the meat quality and defecation condition of livestock and the quality of poultry eggs by improving the absorption rate of the active ingredients of shell and rice bran in the body.

Description

Fermented shell feed using lactic acid bacteria and their preparation method {Fermented feedstuffs of seashells using lactic acid bacteria and their preparation method}

The present invention relates to a shellfish fermented feed using lactic acid bacteria and a method for manufacturing the same.

From the quantitative consumption of meat in the past, there is a tendency to increase the quality consumption and demand for hygienic, safe and high-quality meat. Accordingly, it is necessary to search and develop natural additives and feeds with safe and eco-friendly quality.

Shells are called clam shells or shells, and are by-products of abalone, oysters, or clams. These shells are classified as industrial waste and most of them are disposed of in landfills. In relation to the method of recycling some shells, technologies for recycling shells are being developed, such as a method for manufacturing organic fertilizer using shells, a method for manufacturing inorganic thermal insulation materials using shells, and a technology to utilize the building materials of shells.

Rice bran generated during the milling process of rice contains more than 40 kinds of proteins, lipids, B vitamins, minerals, fibers, and very useful trace elements. Recently, various functionalities of rice bran ingredients have been studied, and in particular, anti-stress effects, antioxidant effects, and immunity enhancing effects on rice bran, which are the inner bran of rice among rice bran, have been reported.

When raising livestock such as chickens and pigs, feed is fed to effectively supply nutrients to livestock. At this time, it is possible to improve the productivity of livestock by supplying calcium using shells or by supplying effective nutrients using rice bran.

In the case of calcium, because of its high reactivity, carbonate (CaCO3; limestone, marble, calcite, calcite, etc.), sulfate (CaSO4; gypsum, etc.), fluoride (CaF2; fluorite, etc.), silicate (CaSiO3 (calcium metasilicate), Ca2SiO4 ( It is a compound such as calcium orthosilicate or dilime silicate), 3CaOSiO2 (trisilicate silicate), etc.; plagioclase feldspar, wollastonite, etc.) Calcium is an essential element for livestock. In animals, it is mainly combined with phosphoric acid, and 99% of it is used to form bones, and about 2% is present in cells and blood inside and outside cells, affecting the physiological functions of the body. When calcium is insufficient, the calcium stored in the bones escapes into the blood and the bones become weak. The most important function of calcium is the formation of bones. The skeleton supports the animal's weight and allows it to perform activities. If there is insufficient calcium in the growing livestock, calcifications of the skeleton are difficult, and the morphology of the bones is incomplete. Calcium carbonate (CaCO3) is the main component of shells, and the calcium content is about 35-40%, and it is used as shell feed.

In order to use the calcium of the shell, the shell was treated with hydrochloric acid to extract calcium, or treated and sterilized with high heat of 1,000° C. or more, and used as a powder. However, when hydrochloric acid is used, there is a problem of environmental pollution of residual chlorine and hydrochloric acid is limited as prohibited items for livestock feeding. In addition, the separation method through high heat requires a huge amount of thermal energy, so economical efficiency is low, and there is a problem in that it is not easy to apply because the facility cost is huge.

In addition, in the case of rice bran or rice bran, research has been conducted to use it as animal feed, but it was difficult to fully utilize its functionality due to the deterioration of digestion and absorption in the body.

And when shells or rice bran are powdered and supplied to livestock, their absorption is low, which causes digestive problems, and the quality deteriorates, so livestock farmers tend to avoid them. Existing inorganic substances for feed addition are classified into feed after the crushing process, so there is a problem in fluidity and absorbability depending on the shape and state of the particles. Furthermore, the low absorbency for livestock has a problem in reducing the quality of livestock, so there is a limitation in using shells and rice bran, which are functional materials.

Republic of Korea Patent Publication No. 10-2010-0027280

Accordingly, an object of the present invention devised to solve the above problems is to provide livestock feed and feed additives by fermenting shells using lactic acid bacteria.

In addition, another object of the present invention is to provide a livestock feed and feed additive capable of increasing the absorption of useful components in the body by feeding the fermented product of shell and rice bran to livestock.

According to an embodiment of the present invention in order to achieve the above object, the method comprising: pulverizing the shells of oysters or clams using a hammer drill to prepare shell powder; mixing rice bran and lactic acid bacteria mixture with the shell powder; fermenting the mixed materials to prepare a fermented product; mixing the fermented product into roughage; It provides a method for producing fermented shellfish feed using lactic acid bacteria comprising a.

Here, rice bran may be mixed in a weight ratio of 2:1 to 4:1 with respect to the shell powder.

In addition, the lactic acid bacteria mixture is Pediococcus acidilactici, Bacillus subtilis, Streptomyces sp), Aspergillus duck material (Aspergillus oryzae), Clostridium genus ( Clostridium sp), Lactobacillus bulgaricus (Lactobacillus bulgaricus) or Saccharomyces cerevisiae (Saccharomyces cerevisiae) may be a culture medium cultured by inoculating at least one strain.

In addition, the fermented product may be mixed in an amount of 0.001 to 3 parts by weight of the fermented product based on 100 parts by weight of the roughage.

In addition, the fermented product may be a feed additive composition.

In addition, the fermented product may further include an enzyme powder and a mineral.

According to an embodiment of the present invention, there is provided a fermented shellfish feed using lactic acid bacteria comprising fermented shell lactic acid bacteria as an active ingredient.

Here, the fermented shell lactic acid bacteria may be fermented including rice bran.

According to the present invention as described above, by improving the absorption rate of the active ingredients of shell and rice bran in the body, it is possible to improve the meat quality and defecation condition of livestock and improve the quality of poultry eggs.

The effects according to the present invention in the above are not limited to the above, and other effects of the present invention can be clearly identified by those of ordinary skill in the art through the examples and claims to be described later. can be understood

1 shows the results of comparing the meat quality of pigs fed with fermented shellfish feed using lactic acid bacteria of the present invention and commercially available pigs.
Figure 2 shows the results of comparing the fatty acid content of pigs fed a shellfish fermented feed using lactic acid bacteria of the present invention and commercially available pigs.

Hereinafter, the present invention will be described in more detail.

The present invention relates to a shellfish fermented feed using lactic acid bacteria and a method for manufacturing the same. It is characterized by improved absorption.

In addition, the present invention can improve the absorption rate of rice bran nutrients in livestock by converting the nutrients into a water-soluble form by microbial fermentation of rice bran.

More specifically, the present invention provides a method for preparing a shellfish fermented feed using lactic acid bacteria.

The manufacturing method according to the present invention comprises the steps of pulverizing the shells of oysters or clams using a hammer drill to prepare shell powder; mixing rice bran and lactic acid bacteria mixture with the shell powder; fermenting the mixed materials to prepare a fermented product; It includes; mixing the fermented product with roughage.

When explaining the method for producing fermented shellfish feed using lactic acid bacteria of the present invention in more detail, first, shell powder is prepared by grinding the shells of oysters or clams using a hammer drill. It is preferable to prepare the shells of oysters or clams by washing them, and the washed oysters or shells dry the remaining moisture at room temperature.

Washed and dried oysters or clam shells are crushed and powdered. In this case, an automated hammer drill may be used as the crusher, but a known crusher may also be used. In the present invention, the shell powder may be crushed to have a diameter of 0.1 to 1 cm with a crusher, but is not limited thereto.

Next, rice bran is mixed with shell powder. Rice bran has a size similar to that of shell powder, and a powder having a diameter of 0.1 to 1 cm can be used. In the present invention, rice bran is preferably mixed in a weight ratio of 2:1 to 4:1 with respect to the shell powder, and more preferably 3:1.

After mixing shell powder and rice bran powder, mix lactic acid bacteria mixture. The lactic acid bacteria mixture may use a culture medium cultured by inoculating a Pediococcus acidilactici strain. In the present invention, Pediococcus acidilactisi is a lactic acid bacterium to convert shell powder and rice bran powder into a water-soluble state to improve absorption in livestock. In the present invention, in addition to Pediococcus acidilactici, Bacillus subtilis, Streptomyces sp, Aspergillus oryzae, Clostridium sp ), Lactobacillus bulgaricus (Lactobacillus bulgaricus), Saccharomyces cerevisiae (Saccharomyces cerevisiae) may be used further including at least one of, but is not limited thereto.

Meanwhile, in the present invention, the enzyme powder and the mineral may be mixed and fermented. In the present invention, the enzyme powder preferably uses pectinase and endoproteases. These pectinase and endoprotease are enzymes optimized for livestock feed among various enzymes, and can increase the protein content and mineral content in the feed by decomposing the protein contained in the main material during the fermentation process. Although not limited thereto, it is preferable to use a mixture of 40 to 60% by weight of pectinase and 40 to 60% by weight of endoprotease as an enzyme, and more preferably, pectinase and endoprotease are mixed in a ratio of 50:50 that can be used For example, a commercial product in which pectinase and endoproteases are mixed may be used.

In the present invention, the mineral is a material separated from ores, and may be used as a trace mineral component in the body. For example, sodium bicarbonate (bicarbonate), bentonite (bentonite), magnesium oxide, a mineral preparation consisting of a complex mineral, zinc, copper, cobalt, a mineral preparation consisting of a trace mineral consisting of selenium may be used, but is not limited thereto. Preferably, a commercially available mineral preparation can be used.

Next, the mixed ingredients are fermented. In the present invention, fermentation may be performed at room temperature. Preferably, it may be fermented at 10 to 25 °C for 2 to 10 days.

And by mixing the fermented fermented product with roughage, it is possible to prepare a shell fermented feed. Here, the fermented product is preferably mixed in an amount of 0.001 to 3 parts by weight of the fermented product based on 100 parts by weight of the roughage, and more preferably 0.2 to 1 parts by weight. In the present invention, the feed can be fed by drying the moisture content to 12% or less, but the present invention is not limited thereto.

Since the fermented shellfish of the present invention has the effect of improving the absorption rate of livestock by converting the calcium of the shell into a water-soluble form of calcium, it can be used as a feed composition and a feed additive composition for improving the quality and health of livestock.

Specifically, the feed containing the fermented shellfish according to the present invention as an active ingredient can be prepared in various types of feed known in the art, and preferably, a concentrated feed, roughage or special feed may be included.

In the enriched feed, seed fruits containing grains such as wheat, oats and corn, rice bran, wheat bran, and barley bran as by-products obtained from refining grains, soybeans, fluids, sesame seeds, linseed seeds, coco palms, etc. are extracted. Fish solvable, meat powder, which is a concentrated product of fish meal, fish waste, and fresh liquid obtained from fish, fish meal, fish waste, and residual starch, which is the main component of starch residue, which is the remainder of starch residue obtained by subtracting starch from sweet potatoes and potatoes. Animal feed such as dried whey, yeast, chlorella, seaweed, etc.

Forage includes raw grass feed such as wild grasses, grasses, and green cuttings, turnips for feed, beets for feed, root vegetables such as ruther bearger, a type of turnip, silage feed that is fermented with lactic acid after filling silos with raw herbs, green crops, and grain; These include wild grass, hay cut and dried, straw from breeders, and leaves from legumes. In the present invention, generally used roughage or compounded feed can be used. Preferably, it contains crude protein, crude fat, crude fiber, crude flour, calcium and phosphorus, and may include vitamins, calcium agents, sugars, blood substitutes, minerals and organic acids, etc. to improve the health and quality of livestock.

In special feed, mineral feed such as rock salt, urea feed such as urea or its derivative diureide isobutane, and natural feed ingredients are added in a small amount to supplement the ingredients that are likely to be lacking when only natural feed ingredients are mixed, or to improve the storage of feed. There are feed additives, dietary supplements, etc.

The feed composition according to the present invention can be used as a variety of feed additives.

In the present invention, 'feed additive' refers to a substance added to feed for the purpose of various effects such as nutrient supplementation and weight loss prevention, improvement of digestibility of fiber in feed, improvement of oil quality, prevention of reproductive disorders and improvement of fertility rate, prevention of high temperature stress in summer . The feed additive of the present invention corresponds to an auxiliary feed under the Feed Management Act, and is a mineral preparation such as sodium bicarbonate (bicarbonate), bentonite, magnesium oxide, and composite minerals, and trace minerals such as zinc, copper, cobalt, and selenium. Preparations, kerotene, vitamin E, vitamins A, D, E, nicotinic acid, vitamins such as vitamin B complex, protective amino acids such as methionine and lyic acid, protective fatty acids such as fatty acid calcium salt, probiotics (lactic acid bacteria), yeast culture Water, live bacteria such as mold fermented products, yeast agents, and the like may be further included.

The feed composition according to the present invention is not particularly limited as long as it is an individual for the purpose of improving the quality of livestock and livestock products through increased absorption of nutrients in the body, and any type of feed composition is applicable. The subject may be an animal, such as a mammal, including cattle, pigs, horses, chickens, cats, dogs, rats and mice as non-primates, and cynomolgus, monkeys and chimpanzees as monkeys as primates. In another embodiment, the subject may be a horse, a cow, a pig, a chicken, or a dog or a cat as a livestock animal.

The dosage of the feed according to the present invention may vary depending on factors such as the species, size, weight, and age of the animal. In principle, a typical dosage may range from 0.001 to 1000 g per animal/day, but is not limited thereto.

In the case of pigs fed the feed prepared in the present invention, compared to normal pigs, the growth was smooth, diarrhea disappeared and normal stools were seen during development, and the amount and odor of stool were greatly reduced. The meat of the pigs fed the feed of the present invention had uniform marbling of meat quality and the odor was significantly reduced compared to that of general pork. As a result of physicochemical analysis of meat quality, calcium content increased significantly (73.12: 100.17 mg/kg), saturated fatty acid content decreased (38.92: 37.15%), and unsaturated fatty acid content increased (61.01: 62.85%).

In addition, in the case of eggs, the size of the egg and the yolk increased, the fishy smell decreased, and the content of unsaturated fatty acids and calcium increased.

Hereinafter, embodiments of the present invention will be described in more detail with reference to the accompanying drawings. However, the following examples are only intended to embody the contents of the present invention, and the present invention will not be limited thereby.

<Example 1> Feed preparation and feeding

1 kg of washed and dried oyster shells and clam shells were pulverized using a hammer drill, and rice bran was mixed therewith. The mixing ratio was 3:1 of crushed oyster shells, clam shells, and rice bran by weight.

Thereafter, 40 L of a lactic acid bacteria mixture culturing Pediococcus acidilactici in the shell powder and rice bran powder mixture, 300 g of a pectinase and endoprotease (Australian enzyme) mixture, and 300 g of a mineral were mixed, followed by 3 at room temperature. Fermented products were prepared by daily fermentation.

The prepared fermented product was dried with warm air, mixed in a weight ratio of 1 to 100 weight ratio of the existing feed, and fed to chickens, pigs, and cows.

<Example 2> Feed feeding results

After feeding the feed prepared in Example 1 to chickens, pigs, and cows raised for 50 days, the defecation, egg condition, and meat quality of the individual who ate the feed were checked.

In the case of laying hens, midwives of eggs were prevented, and as a result of feeding chickens with diarrhea, diarrhea stopped after 7 days, and normal bowel movements and normal spawning were confirmed. The odor was reduced in defecation.

In the case of pigs, it had the effect of improving the meat quality. As a result of comparing the meat quality of pigs fed the feed of the present invention with that of pigs on the market, it was confirmed that the calcium content was improved in pigs fed the feed of the present invention (FIG. 1). This was determined to be the effect of calcium absorption into the body by feeding the feed of the present invention.

In addition, it was confirmed that the pig fed the feed of the present invention improved saturated fatty acid and unsaturated fatty acid compared to normal pigs (FIG. 2).

As described above, although specific embodiments of the present invention have been described in detail, those skilled in the art who understand the spirit of the present invention may add, change, delete, etc. other components within the scope of the same spirit, and other degenerate inventions However, other embodiments included within the scope of the present invention may be easily proposed. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention is indicated by the following claims rather than the above detailed description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents are included in the scope of the present invention. should be interpreted as

Claims (8)

pulverizing the shells of oysters or clams using a hammer drill to prepare shell powder;
Mixing the culture medium by inoculating the shell powder with rice bran powder and Pediococcus acidilactici strain;
fermenting the mixed materials to prepare a fermented product;
mixing the fermented product into roughage;
including,
In the step of producing the fermented product, the Pediococcus acidilactisi is a shell fermented feed manufacturing method using lactic acid bacteria, characterized in that it improves the absorption rate in livestock by converting shell powder and rice bran powder into a water-soluble state.
The method of claim 1,
The method for producing a shellfish fermented feed using lactic acid bacteria, characterized in that the rice bran powder is mixed in a weight ratio of 2:1 to 4:1 with respect to the shell powder.
delete The method of claim 1,
The fermented product is a method for producing a shellfish fermented feed using lactic acid bacteria, characterized in that mixed with 0.001 to 3 parts by weight of the fermented product with respect to 100 parts by weight of the roughage.
The method of claim 1,
The fermented product is a method for producing a shell fermented feed using lactic acid bacteria, characterized in that the feed additive composition.
The method of claim 1,
The fermented product is a shell fermentation feed manufacturing method using lactic acid bacteria, characterized in that it further comprises an enzyme powder and a mineral.
delete delete

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