KR102398670B1 - Composition for detoxifying cadmium comprising aster glehnii extract - Google Patents

Abstract

The present invention relates to a composition for alleviating cadmium poisoning, comprising an extract of sagebrush extract or eoseongcho extract. It can be utilized as a composition for liver protection from liver toxicity and heavy metal accumulation caused by heavy metals.
The liver can be effectively protected from heavy metal toxicity and accumulation by using a natural extract such as an extract of sagebrush extract or eoseongcho extract according to the present invention.

Description

Composition for alleviating cadmium poisoning, including extract of Artemisia wormwood

The present invention relates to a composition for alleviating cadmium poisoning, comprising an extract of sagebrush.

Cadmium (Cd) is a first-class carcinogen and highly toxic metal. It is very harmful to the human body and causes symptoms such as breathing difficulties and liver dysfunction. It is the cause of 'itai-itai disease', one of the four major pollution diseases, and because it has similar properties to zinc required by the human body, it is ingested into the body and causes kidney failure or osteomalacia by interfering with calcium absorption. In contrast to zinc (Zn), which is in the same group on the periodic table, as an essential trace element that plays an important role in various processes in the body, cadmium is very toxic and accumulates in the body, causing various poisoning symptoms.

Cadmium enters the body through some foods (eg, fish and shellfish, animal kidneys or liver) or contaminated water, and accumulates in the liver and kidneys (kidneys), causing damage to these organs. In particular, kidney damage causes various diseases by excreting various proteins and sugars essential for human body function. Cadmium poisoning is accompanied by diarrhea, abdominal pain, severe vomiting, and can lead to impaired reproductive function and infertility, liver and kidney disorders, damage to the central nervous system and immune system, mental illness, high blood pressure, and cancer. Long-term ingestion even in small amounts can cause poisoning symptoms.

Aster glehni F.Schmidt is also known as poker greens, sagebrush, and goumerinamul. Poker sprouts, a special product of wild vegetables in Ulleungdo, are about 1 m in height, have fine hairs on the stem, and have rhizomes growing sideways. . The leaves are alternate phyllotaxis, and when the flowers bloom, the lower leaves fall off and are oval with short stems. Poker herbs have been studied for cough, diuretic, asthma, whitening, anti-wrinkle effects, suppression of adipocyte differentiation and anti-inflammatory effects.

Houttuynia Cordata ) is a perennial herb belonging to the family Trifoliumaceae, known by its pseudonym as Yakmomil, and grows in shady forests. Underground stems are long, thin, and white. The stem is upright, 20-50cm high, has several vertical lines, and smells like fishy smell. It is known to have antipyretic, pneumonia, antibacterial, diuretic, analgesic and other effects.

However, sagebrush and eoseongcho are only limited in their use as foods such as greens, such as roasted or fried young leaves in spring, and the effect of cadmium poisoning on their extracts is not known yet.

Republic of Korea Patent Publication No. 10-2016-0140417 (published on December 07, 2016)

It is an object of the present invention to provide a composition for alleviating cadmium poisoning comprising a natural extract as an active ingredient.

The composition for alleviating cadmium poisoning according to the present invention may include an extract of sagebrush as an active ingredient.

The composition for alleviating cadmium poisoning for the accumulation of heavy metals according to the present invention may include an extract of sagebrush and eosinthia extract as active ingredients.

The Artemisia serrata extract or Eosinosaurus extract according to the present invention protects liver cells from heavy metal toxicity and can inhibit the accumulation of the heavy metal, and is used as a composition for alleviating cadmium poisoning from liver toxicity and heavy metal accumulation caused by heavy metals can be

The liver can be effectively protected from heavy metal toxicity and accumulation by using a natural extract such as a wormwood extract or eoseongcho extract according to the present invention.

1 is a graph showing the results of cytotoxicity evaluation of the extract and eoseongcho extract according to an experimental example of the present invention.
Figure 2 is a graph showing the HepG2 cell protective effect of the extract and eoseongcho extract according to an experimental example of the present invention.
Figure 3 is a graph showing the inhibitory effect of the extract of sagebrush extract and eoseongcho extract on cadmium uptake in HepG2 cells according to an experimental example of the present invention.

Hereinafter, the present invention will be described in detail.

The present inventors have completed the present invention by confirming the liver protection effect and the heavy metal accumulation inhibitory effect from heavy metal toxicity such as cadmium in the extracts of Artemisia sagebrush and Eosongcho extract.

As used herein, the term "extract" refers to a substance obtained by extracting a component of a natural product regardless of an extraction method, an extraction solvent, an extracted component, or the form of an extract. It may include any material obtainable by processing or processing by the method.

The present invention provides a composition for alleviating cadmium poisoning comprising an extract of sagebrush as an active ingredient.

The sagebrush may be collected or cultivated in nature, or purchased commercially. The extract may be obtained from extracts of flowers, leaves, stems, roots, or mixtures of these parts.

The extract may be water, a C1 to C4 alcohol, or an aqueous solution thereof as a solvent, and preferably a hot water extract.

Specifically, the extract may be a hot water extract obtained by hot water extraction at a temperature of 70 to 90° C., more preferably 80° C. for 5 to 7 hours, more preferably 6 hours.

The extract may be extracted according to a method commonly used in the art, for example, hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method, etc., which are performed alone or two or more methods It can be performed in combination.

The composition for alleviating cadmium poisoning according to the present invention may further include an extract of eoseongcho.

The eoseongcho may also be collected or cultivated in nature, or purchased commercially. The eoseongcho extract may be a flower, leaf, stem, root, or a mixture of these parts of the eoseongcho extract.

The extract may be water, C1 to C4 alcohol or an aqueous solution thereof as a solvent, and may be preferably a hot water extract.

Specifically, the eoseongcho extract may be a hot water extract obtained by hot water extraction at a temperature of 70 to 90 ℃, more preferably 80 ℃ for 5 to 7 hours, more preferably for 6 hours.

The extract can be extracted according to a method commonly used in the art, for example, there are hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method, etc., these are performed alone or by using two or more methods in combination can be performed.

The composition for alleviating cadmium poisoning according to the present invention may include only a single extract of the sagebrush extract or eoseongcho extract, and may include a mixed extract obtained by mixing the sagebrush extract or eoseongcho extract in a certain ratio, respectively, It may include a complex extract extracted by mixing Artemisia sagebrush and Eoseongcho.

In the composition for alleviating cadmium poisoning according to the present invention, the wormwood extract or the extract of Eosinosaurus may have an effect of protecting liver cells from toxicity of heavy metals and inhibiting the accumulation of the heavy metals in the cells. According to an experimental example of the present invention, it was confirmed that the extract exhibited the liver cell protective effect in cadmium-treated HepG2 cells, and suppressed cadmium accumulation.

The heavy metal may be one or more selected from cadmium (Cd) and lead (Pb), in addition to mercury (Hg), beryllium (Be), barium (Ba), arsenic (As), bismuth (Bi), antimony (Sb) It may include, but is not limited to.

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In the composition for alleviating cadmium poisoning according to the present invention, the composition may be a pharmaceutical composition or a health food composition. The sagebrush extract or Eoseongcho extract protects liver cells from the toxicity of heavy metals, and inhibits the accumulation of the heavy metals in the cells, so it can be used as a pharmaceutical composition for liver protection or a health food composition for liver protection.

The pharmaceutical composition according to the present invention may be prepared according to a conventional method in the pharmaceutical field. The pharmaceutical composition may be combined with a suitable pharmaceutically acceptable carrier according to the formulation, and if necessary, excipients, diluents, dispersants, emulsifiers, buffers, stabilizers, binders, disintegrants, solvents, etc. may be prepared further comprising there is. The appropriate carrier and the like do not inhibit the activity and properties of the extracts of Artemisia serrata or Eosinosaurus extract according to the present invention, and may be selected differently depending on the dosage form and formulation.

The pharmaceutical composition according to the present invention can be applied in any dosage form, and more specifically, it can be used by formulating oral dosage forms, external preparations, suppositories, and parenteral dosage forms of sterile injection solutions according to conventional methods.

The solid dosage form among the oral dosage forms is in the form of tablets, pills, powders, granules, capsules, etc., and at least one or more excipients, for example, starch, calcium carbonate, sucrose, lactose, sorbitol, mannitol, cellulose, gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc may be included. In addition, the capsule formulation may further include a liquid carrier such as fatty oil in addition to the above-mentioned substances.

Among the oral dosage forms, liquid formulations include suspensions, solutions, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients, for example, wetting agents, sweeteners, fragrances, preservatives, etc. may be included. there is.

The parenteral formulation may include a sterile aqueous solution, a non-aqueous solution, a suspension, an emulsion, a freeze-dried formulation, and a suppository. Non-aqueous solvents and suspending agents include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, Tween 61, cacao butter, laurin fat, glycerogelatin, and the like can be used. It is not limited thereto, and any suitable agent known in the art may be used.

In addition, the pharmaceutical composition according to the present invention may further add calcium or vitamin D ₃ or the like to enhance therapeutic efficacy.

The pharmaceutical composition according to the present invention may be administered in a pharmaceutically effective amount.

As used herein, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment and not to cause side effects.

The effective dose level of the pharmaceutical composition may be determined according to the purpose of use, the age, sex, weight and health status of the patient, the type of disease, the severity, the activity of the drug, the sensitivity to the drug, the administration method, the administration time, the administration route and the excretion rate, the treatment The duration, formulation or concomitant use may be determined differently depending on factors including drugs and other factors well known in the medical field. For example, although not constant, generally 0.001 to 100 mg/kg, preferably 0.01 to 10 mg/kg, may be administered once to several times a day. The above dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition according to the present invention may be administered by an appropriate administration route according to the form of the formulation, and may be administered via various routes, either oral or parenteral, as long as it can reach the target tissue. The administration method does not need to be particularly limited, and for example, oral, rectal or intravenous, muscle, skin application, subcutaneous, respiratory inhalation, intrauterine dural or intracerebroventricular injection, etc. are administered in a conventional manner. can be

The pharmaceutical composition according to the present invention may be used alone for liver protection from toxicity or accumulation of heavy metals, or may be used in combination with surgery or other drug treatment.

In the health food composition according to the present invention, the health food may be prepared in the form of powder, granules, tablets, capsules, syrup, or beverages for the purpose of liver protection, and there is no limitation in the form that the food can take, It can include any food with meaning. For example, beverages and various drinks, fruits and their processed foods (canned fruit, jam, etc.), fish, meat and their processed foods (ham, bacon, etc.), breads and noodles, cookies and snacks, dairy products (butter, cheese, etc.) ), etc. are possible, and may include all functional foods in a conventional sense. It may also include food used as feed for animals.

The health food composition according to the present invention may be prepared by further including pharmaceutically acceptable food additives (food additives) and other suitable auxiliary ingredients commonly used in the art.

Unless otherwise specified, the suitability as a food additive can be judged according to the standards and standards for the relevant item in accordance with the general rules and general test methods of the Food Additives Code approved by the Food and Drug Administration. The items listed in the 'Food Additives Code' include, for example, chemical compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamic acid; natural additives such as persimmon pigment, licorice extract, crystalline cellulose, high pigment, and guar gum; Mixed preparations, such as a sodium L-glutamate preparation, a noodle-added alkali agent, a preservative preparation, and a tar color preparation, etc. are mentioned.

The other auxiliary ingredients include, for example, flavoring agents, natural carbohydrates, sweeteners, vitamins, electrolytes, coloring agents, pectic acid, alginic acid, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonation agents, etc. may further contain. In particular, as the natural carbohydrate, monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol can be used. , As the sweetener, natural sweeteners such as taumatine and stevia extract, or synthetic sweeteners such as saccharin and aspartame can be used.

The effective dose of the extract of Artemisia serrata or Eosinosaurus extract contained in the health food according to the present invention can be appropriately adjusted according to the purpose of its use, such as liver protection, in particular, liver protection from toxicity or accumulation of heavy metals. The composition has the advantage of not having side effects that may occur during long-term administration of general drugs by using natural products and food as raw materials, has excellent portability, and can be ingested as an adjuvant for liver protection.

In addition, the present invention provides a composition for alleviating cadmium poisoning against the accumulation of heavy metals, which includes an extract of sagebrush and Eosongcho extract as active ingredients.

The heavy metal may be one or more selected from cadmium (Cd) and lead (Pb), in addition to mercury (Hg), beryllium (Be), barium (Ba), arsenic (As), bismuth (Bi), antimony (Sb) It may include, but is not limited to.

The sagebrush extract and Eoseongcho extract can protect liver cells from the toxicity of heavy metals, and inhibit the accumulation of the heavy metals in the cells. Corresponding features may be substituted for the above-mentioned parts.

Hereinafter, examples will be described in detail to help the understanding of the present invention. However, the following examples are merely illustrative of the content of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

<Example 1> Preparation of extracts of sagebrush and Eoseongcho

After crushing the dried wormwood and seaweed, it was added with 10-fold distilled water, and then extracted at a temperature of 80° C. for 6 hours. The extracted sample was filtered with Advantec (No. 5A, Tokyo, Japan) filter paper, concentrated using a vacuum concentrator, and then freeze-dried and used as a sample for analysis.

<Experimental Example 1> Toxicity evaluation of extracts of Mugwort wormwood extract and Eoseongcho extract

HepG2 cells were used to evaluate the toxicity of the extracts of Artemisia serrata and Eosinosaurus extracts on hepatocytes.

MTT assay was performed to measure the cytotoxicity of the extracts of sagebrush extract and eosinosaurus extract against cadmium-treated HepG2 cells. HepG2 cells were aliquoted to 2×10 ⁵ cells/well in a 24-well plate and cultured for 20 to 24 hours in a 5% CO ₂ incubator, and then, extract After treatment by concentration, 5% CO ₂ Incubated for 20 to 24 hours in an incubator. 50 μl of MTT (5 mg/ml) solution dissolved in PBS was put into each well and reacted in an incubator for 4 hours. After that, the supernatant was removed, and 200 μl of DMSO was added to each well to dissolve the formed formazan crystal, and absorbance was measured with an ELISA reader at a wavelength of 550 nm. The measured formazan production level was expressed as a percentage (%) compared to the value of normal cells.

1 is a graph showing the results of cytotoxicity evaluation of the extract and eoseongcho extract according to an experimental example of the present invention.

As a result of measuring the cytotoxicity of the sagebrush extract and Eoseongcho extract on HepG2 cells, as shown in FIG.

< Experimental example 2> scumbag Extracts and Eoseongcho extract Liver protection against cadmium check the effect

HepG2 cells were used to confirm the liver-protective effect of sagebrush extracts and eosin-chief extracts against cadmium.

One. HepG2 for cadmium toxicity in cells. scumbag Confirmation of the protective effect of extracts and eoseongcho extracts

MTT assay was performed to measure the cytoprotective ability of the extracts of sagebrush and Echo extract against cadmium-treated HepG2 cells. HepG2 cells were aliquoted to 2×10 ⁵ cells/well in a 24-well plate and cultured in a 5% CO ₂ incubator for 20 to 24 hours _. Pre-cultured for 20-24 hours in an incubator. CdCl ₂ reacted for 24 hours after treatment so that the final concentration of 10 μM, 50 μl of MTT (5 mg/ml) solution dissolved in PBS was put into each well and reacted in an incubator for 4 hours. Then, the supernatant was removed, and 200 μl of DMSO was added to each well to dissolve the formed formazan crystal, and absorbance was measured with an ELISA reader at a wavelength of 550 nm. The measured formazan production level was expressed as a percentage (%) compared to the value of normal cells.

Figure 2 is a graph showing the HepG2 cell protective effect of the extract and eoseongcho extract according to an experimental example of the present invention.

As a result of measuring the cytoprotective effect of the extracts of sagebrush extract and eoseongcho extract in HepG2 cells treated with cadmium, as shown in FIG.

In the case of sagebrush, the cell viability increased by about 50.38% compared to the control treated with cadmium at a concentration of 500 μg/ml, resulting in a cytoprotective effect. It increased by 14.56%, showing a cytoprotective effect.

2. HepG2 for cadmium uptake in cells. scumbag Confirmation of the inhibitory effect of the extract and Eosongcho extract

ICP-MS (Agilent 7500cx) was used to confirm the inhibitory effect of cadmium uptake on HepG2 cells of the extracts of Artemisia sagebrush and Echo extract. HepG2 cells were aliquoted to 5×10 ⁵ cells/well in a 6-well plate and pre-cultured for 20 to 24 hours in a 5% CO ₂ incubator. After treatment according to the concentration of the wormwood extract and the Eoseongcho extract, it was cultured in a 5% CO ₂ incubator for 20 to 24 hours. CdCl ₂ reacted for 24 hours after treatment so that the final concentration of 10 μM, washed twice with PBS, and the cells were collected with PBS. The number of cells was counted at 1×10 ⁶ cell/ml, diluted with 1.8 ml of aqua regia (hydrochloric acid 3: nitric acid 1) and 18 ml of distilled water, and reacted at 80° C. for 3 hours. After filtering, cadmium in the solution was measured using ICP-MS.

Figure 3 is a graph showing the inhibitory effect of the extract of sagebrush extract and eoseongcho extract on cadmium uptake in HepG2 cells according to an experimental example of the present invention.

As a result of measuring the cell accumulation amount inhibitory effect of the sagebrush extract and the Echo extract in HepG2 cells treated with cadmium, the amount of cadmium was measured to be 3.54 ppb in 1×10 ⁶ cells not treated with cadmium, as shown in FIG. 3, and cadmium was When treated, it was confirmed that cadmium of 37.53 ppb was measured and accumulated.

In the case of treatment with the sagebrush extract, it was measured to be 15.21 ppb at a concentration of 500 μg/ml, and it was confirmed that the accumulation amount was reduced by 59.52%. It was confirmed that the reduction was 55.62%, and it was confirmed that the accumulation amount of the extracts was decreased in a concentration-dependent manner. It can be confirmed that Artemisia sagebrush extract and Ewoongcho extract inhibit the accumulation of cadmium in HepG2 hepatocytes.

As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, it is clear that this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. Do. That is, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (7)

A composition for alleviating cadmium poisoning, comprising an extract of sagebrush as an active ingredient. The method of claim 1,
The composition is
A composition for alleviating cadmium poisoning, characterized in that it further comprises an extract of Eoseongcho. The method of claim 1,
The extract is
A composition for alleviating cadmium poisoning, characterized in that hot water extraction at a temperature of 70 to 90° C. for 5 to 7 hours. The method of claim 1,
The composition is
A composition for alleviating cadmium poisoning, characterized in that it is administered to a patient who needs to remove cadmium due to cadmium accumulation in the body than normal. The method of claim 1,
The composition is a composition for alleviating cadmium poisoning, characterized in that it is a health food composition, a pharmaceutical composition, or a composition for adding feed. A method of alleviating cadmium poisoning, comprising administering an extract of sagebrush to a non-human subject. 7. The method of claim 6,
Cadmium poisoning alleviation method, characterized in that it further comprises an extract of Eoseongcho in addition to the extract of the sagebrush extract.

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