KR102349860B1 - Composition for enhancing innate immunity and antivirus comprising Benincasae Pericarpium extract as effective component - Google Patents
Composition for enhancing innate immunity and antivirus comprising Benincasae Pericarpium extract as effective component Download PDFInfo
- Publication number
- KR102349860B1 KR102349860B1 KR1020150039180A KR20150039180A KR102349860B1 KR 102349860 B1 KR102349860 B1 KR 102349860B1 KR 1020150039180 A KR1020150039180 A KR 1020150039180A KR 20150039180 A KR20150039180 A KR 20150039180A KR 102349860 B1 KR102349860 B1 KR 102349860B1
- Authority
- KR
- South Korea
- Prior art keywords
- virus
- antiviral
- innate immunity
- extract
- newcastle disease
- Prior art date
Links
- 239000000284 extract Substances 0.000 title claims abstract description 62
- 230000015788 innate immune response Effects 0.000 title claims abstract description 56
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 38
- 239000000203 mixture Substances 0.000 title claims abstract description 27
- 230000002155 anti-virotic effect Effects 0.000 title 1
- 230000000840 anti-viral effect Effects 0.000 claims abstract description 62
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims abstract description 34
- 229910052802 copper Inorganic materials 0.000 claims abstract description 33
- 239000010949 copper Substances 0.000 claims abstract description 33
- 235000013376 functional food Nutrition 0.000 claims abstract description 21
- 229940027779 persimmon extract Drugs 0.000 claims abstract description 19
- 235000011511 Diospyros Nutrition 0.000 claims abstract description 18
- 244000236655 Diospyros kaki Species 0.000 claims abstract description 18
- 230000036541 health Effects 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 18
- 239000008194 pharmaceutical composition Substances 0.000 claims abstract description 18
- 239000004480 active ingredient Substances 0.000 claims abstract description 17
- 241001465754 Metazoa Species 0.000 claims abstract description 15
- 241000700605 Viruses Species 0.000 claims description 42
- 241001529459 Enterovirus A71 Species 0.000 claims description 34
- 241000700584 Simplexvirus Species 0.000 claims description 27
- 241000712461 unidentified influenza virus Species 0.000 claims description 26
- 241000711404 Avian avulavirus 1 Species 0.000 claims description 21
- 230000009385 viral infection Effects 0.000 claims description 20
- 241000711975 Vesicular stomatitis virus Species 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000002775 capsule Substances 0.000 claims description 7
- 239000006187 pill Substances 0.000 claims description 6
- 239000008187 granular material Substances 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 235000013361 beverage Nutrition 0.000 claims description 4
- 239000003085 diluting agent Substances 0.000 claims description 4
- 239000003937 drug carrier Substances 0.000 claims description 4
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 239000003826 tablet Substances 0.000 claims description 4
- 208000036142 Viral infection Diseases 0.000 claims description 3
- 239000000654 additive Substances 0.000 claims description 3
- 235000009508 confectionery Nutrition 0.000 claims description 3
- 235000013305 food Nutrition 0.000 claims description 3
- 238000009472 formulation Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000000996 additive effect Effects 0.000 claims description 2
- 239000000443 aerosol Substances 0.000 claims description 2
- 125000004432 carbon atom Chemical group C* 0.000 claims description 2
- 235000015218 chewing gum Nutrition 0.000 claims description 2
- 229940112822 chewing gum Drugs 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 claims description 2
- 235000015110 jellies Nutrition 0.000 claims description 2
- 239000008274 jelly Substances 0.000 claims description 2
- 239000000829 suppository Substances 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 239000006188 syrup Substances 0.000 claims description 2
- 235000020357 syrup Nutrition 0.000 claims description 2
- 235000004347 Perilla Nutrition 0.000 abstract description 16
- 230000000694 effects Effects 0.000 abstract description 11
- 230000036039 immunity Effects 0.000 abstract description 11
- 208000035473 Communicable disease Diseases 0.000 abstract description 10
- 201000010099 disease Diseases 0.000 abstract description 10
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 10
- 230000002265 prevention Effects 0.000 abstract description 9
- 239000003814 drug Substances 0.000 abstract description 7
- 230000003612 virological effect Effects 0.000 abstract description 7
- 230000002829 reductive effect Effects 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 3
- 238000005728 strengthening Methods 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 244000124853 Perilla frutescens Species 0.000 abstract 2
- 244000131316 Panax pseudoginseng Species 0.000 abstract 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 abstract 1
- 235000003140 Panax quinquefolius Nutrition 0.000 abstract 1
- 235000008434 ginseng Nutrition 0.000 abstract 1
- 239000005090 green fluorescent protein Substances 0.000 description 45
- 208000015181 infectious disease Diseases 0.000 description 27
- 238000004458 analytical method Methods 0.000 description 22
- 210000004027 cell Anatomy 0.000 description 21
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 14
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 14
- 241000229722 Perilla <angiosperm> Species 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 13
- 102000004127 Cytokines Human genes 0.000 description 11
- 108090000695 Cytokines Proteins 0.000 description 11
- 241000709661 Enterovirus Species 0.000 description 10
- 102100026720 Interferon beta Human genes 0.000 description 10
- 108090000467 Interferon-beta Proteins 0.000 description 10
- 229960005486 vaccine Drugs 0.000 description 10
- 102000014150 Interferons Human genes 0.000 description 8
- 108010050904 Interferons Proteins 0.000 description 8
- 229940079322 interferon Drugs 0.000 description 8
- 239000013642 negative control Substances 0.000 description 8
- 230000000770 proinflammatory effect Effects 0.000 description 8
- 238000002073 fluorescence micrograph Methods 0.000 description 7
- 230000006698 induction Effects 0.000 description 7
- 244000052769 pathogen Species 0.000 description 7
- 239000013641 positive control Substances 0.000 description 7
- 238000003556 assay Methods 0.000 description 6
- 238000007796 conventional method Methods 0.000 description 6
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 208000009889 Herpes Simplex Diseases 0.000 description 5
- 241000701074 Human alphaherpesvirus 2 Species 0.000 description 5
- 108090001005 Interleukin-6 Proteins 0.000 description 5
- 102000004889 Interleukin-6 Human genes 0.000 description 5
- 241000725643 Respiratory syncytial virus Species 0.000 description 5
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 5
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 5
- 239000003443 antiviral agent Substances 0.000 description 5
- 230000003833 cell viability Effects 0.000 description 5
- 229940088592 immunologic factor Drugs 0.000 description 5
- 230000001939 inductive effect Effects 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 210000002540 macrophage Anatomy 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 4
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 241000700588 Human alphaherpesvirus 1 Species 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 229940100601 interleukin-6 Drugs 0.000 description 4
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 3
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 3
- 241000709687 Coxsackievirus Species 0.000 description 3
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 235000013355 food flavoring agent Nutrition 0.000 description 3
- 230000028993 immune response Effects 0.000 description 3
- 239000000367 immunologic factor Substances 0.000 description 3
- 239000008101 lactose Substances 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 235000019359 magnesium stearate Nutrition 0.000 description 3
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 3
- 230000003449 preventive effect Effects 0.000 description 3
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 238000002255 vaccination Methods 0.000 description 3
- 239000002435 venom Substances 0.000 description 3
- 231100000611 venom Toxicity 0.000 description 3
- 210000001048 venom Anatomy 0.000 description 3
- 238000003809 water extraction Methods 0.000 description 3
- UBCHPRBFMUDMNC-UHFFFAOYSA-N 1-(1-adamantyl)ethanamine Chemical compound C1C(C2)CC3CC2CC1(C(N)C)C3 UBCHPRBFMUDMNC-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- 229930091371 Fructose Natural products 0.000 description 2
- 239000005715 Fructose Substances 0.000 description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 208000010359 Newcastle Disease Diseases 0.000 description 2
- 101001039853 Sonchus yellow net virus Matrix protein Proteins 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 2
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 235000010443 alginic acid Nutrition 0.000 description 2
- 229920000615 alginic acid Polymers 0.000 description 2
- DKNWSYNQZKUICI-UHFFFAOYSA-N amantadine Chemical compound C1C(C2)CC3CC2CC1(N)C3 DKNWSYNQZKUICI-UHFFFAOYSA-N 0.000 description 2
- 229960003805 amantadine Drugs 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 210000003169 central nervous system Anatomy 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000007123 defense Effects 0.000 description 2
- 230000008260 defense mechanism Effects 0.000 description 2
- 210000002249 digestive system Anatomy 0.000 description 2
- 241001493065 dsRNA viruses Species 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 210000002865 immune cell Anatomy 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 2
- 231100000956 nontoxicity Toxicity 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- VSZGPKBBMSAYNT-RRFJBIMHSA-N oseltamivir Chemical compound CCOC(=O)C1=C[C@@H](OC(CC)CC)[C@H](NC(C)=O)[C@@H](N)C1 VSZGPKBBMSAYNT-RRFJBIMHSA-N 0.000 description 2
- 229960003752 oseltamivir Drugs 0.000 description 2
- 238000010979 pH adjustment Methods 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 239000002574 poison Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 229960003415 propylparaben Drugs 0.000 description 2
- 210000002345 respiratory system Anatomy 0.000 description 2
- 229960000888 rimantadine Drugs 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 229960002920 sorbitol Drugs 0.000 description 2
- 235000010356 sorbitol Nutrition 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 239000000454 talc Substances 0.000 description 2
- 229910052623 talc Inorganic materials 0.000 description 2
- 235000012222 talc Nutrition 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 235000010447 xylitol Nutrition 0.000 description 2
- 239000000811 xylitol Substances 0.000 description 2
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 2
- 229960002675 xylitol Drugs 0.000 description 2
- ARAIBEBZBOPLMB-UFGQHTETSA-N zanamivir Chemical compound CC(=O)N[C@@H]1[C@@H](N=C(N)N)C=C(C(O)=O)O[C@H]1[C@H](O)[C@H](O)CO ARAIBEBZBOPLMB-UFGQHTETSA-N 0.000 description 2
- 229960001028 zanamivir Drugs 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 description 1
- 244000215068 Acacia senegal Species 0.000 description 1
- 235000006491 Acacia senegal Nutrition 0.000 description 1
- 208000034579 Acute haemorrhagic conjunctivitis Diseases 0.000 description 1
- 208000006740 Aseptic Meningitis Diseases 0.000 description 1
- 244000036905 Benincasa cerifera Species 0.000 description 1
- 235000011274 Benincasa cerifera Nutrition 0.000 description 1
- 241000710780 Bovine viral diarrhea virus 1 Species 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- PTHCMJGKKRQCBF-UHFFFAOYSA-N Cellulose, microcrystalline Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC)C(CO)O1 PTHCMJGKKRQCBF-UHFFFAOYSA-N 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 241000710777 Classical swine fever virus Species 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 241000709734 Coxsackievirus A24 Species 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 241000450599 DNA viruses Species 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241001466953 Echovirus Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 1
- 239000004606 Fillers/Extenders Substances 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920000084 Gum arabic Polymers 0.000 description 1
- 206010069767 H1N1 influenza Diseases 0.000 description 1
- 241000711549 Hepacivirus C Species 0.000 description 1
- 208000001688 Herpes Genitalis Diseases 0.000 description 1
- 241000700586 Herpesviridae Species 0.000 description 1
- 208000000440 Herpetic Stomatitis Diseases 0.000 description 1
- 241001207270 Human enterovirus Species 0.000 description 1
- 241000725303 Human immunodeficiency virus Species 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 102000004310 Ion Channels Human genes 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 206010027201 Meningitis aseptic Diseases 0.000 description 1
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 1
- 101001054328 Mus musculus Interferon beta Proteins 0.000 description 1
- 208000009525 Myocarditis Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010067152 Oral herpes Diseases 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- 229920002230 Pectic acid Polymers 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 241000709664 Picornaviridae Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 241001135549 Porcine epidemic diarrhea virus Species 0.000 description 1
- 241000702263 Reovirus sp. Species 0.000 description 1
- 241000711931 Rhabdoviridae Species 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 244000228451 Stevia rebaudiana Species 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 244000299461 Theobroma cacao Species 0.000 description 1
- 235000005764 Theobroma cacao ssp. cacao Nutrition 0.000 description 1
- 235000005767 Theobroma cacao ssp. sphaerocarpum Nutrition 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- MKUXAQIIEYXACX-UHFFFAOYSA-N aciclovir Chemical compound N1C(N)=NC(=O)C2=C1N(COCCO)C=N2 MKUXAQIIEYXACX-UHFFFAOYSA-N 0.000 description 1
- 229960004150 aciclovir Drugs 0.000 description 1
- 229940072056 alginate Drugs 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000001754 anti-pyretic effect Effects 0.000 description 1
- 239000002221 antipyretic Substances 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 235000014121 butter Nutrition 0.000 description 1
- 235000001046 cacaotero Nutrition 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000000378 calcium silicate Substances 0.000 description 1
- 229910052918 calcium silicate Inorganic materials 0.000 description 1
- 235000012241 calcium silicate Nutrition 0.000 description 1
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000011889 copper foil Substances 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 239000012043 crude product Substances 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 230000008029 eradication Effects 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 208000030533 eye disease Diseases 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000007903 gelatin capsule Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 201000004946 genital herpes Diseases 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 239000012676 herbal extract Substances 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229940124590 live attenuated vaccine Drugs 0.000 description 1
- 229940023012 live-attenuated vaccine Drugs 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 229960003511 macrogol Drugs 0.000 description 1
- 239000000845 maltitol Substances 0.000 description 1
- 235000010449 maltitol Nutrition 0.000 description 1
- VQHSOMBJVWLPSR-WUJBLJFYSA-N maltitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-WUJBLJFYSA-N 0.000 description 1
- 229940035436 maltitol Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 230000003446 memory effect Effects 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 235000010981 methylcellulose Nutrition 0.000 description 1
- 229960002216 methylparaben Drugs 0.000 description 1
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 1
- 239000008108 microcrystalline cellulose Substances 0.000 description 1
- 229940016286 microcrystalline cellulose Drugs 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 230000004719 natural immunity Effects 0.000 description 1
- 210000000822 natural killer cell Anatomy 0.000 description 1
- 230000021616 negative regulation of cell division Effects 0.000 description 1
- 231100001083 no cytotoxicity Toxicity 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- -1 pH regulators Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- LCLHHZYHLXDRQG-ZNKJPWOQSA-N pectic acid Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)O[C@H](C(O)=O)[C@@H]1OC1[C@H](O)[C@@H](O)[C@@H](OC2[C@@H]([C@@H](O)[C@@H](O)[C@H](O2)C(O)=O)O)[C@@H](C(O)=O)O1 LCLHHZYHLXDRQG-ZNKJPWOQSA-N 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000005453 pelletization Methods 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 239000010318 polygalacturonic acid Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 229940092385 radish extract Drugs 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- HELXLJCILKEWJH-NCGAPWICSA-N rebaudioside A Chemical compound O([C@H]1[C@H](O)[C@@H](CO)O[C@H]([C@@H]1O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)O[C@]12C(=C)C[C@@]3(C1)CC[C@@H]1[C@@](C)(CCC[C@]1([C@@H]3CC2)C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O1)O)[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HELXLJCILKEWJH-NCGAPWICSA-N 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
- 229940081974 saccharin Drugs 0.000 description 1
- 235000019204 saccharin Nutrition 0.000 description 1
- 239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 description 1
- 229940001584 sodium metabisulfite Drugs 0.000 description 1
- 235000010262 sodium metabisulphite Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000003594 spinal ganglia Anatomy 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 208000003265 stomatitis Diseases 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 201000010740 swine influenza Diseases 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 239000000892 thaumatin Substances 0.000 description 1
- 235000010436 thaumatin Nutrition 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 230000029812 viral genome replication Effects 0.000 description 1
- 230000005727 virus proliferation Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/324—Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Polymers & Plastics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medical Informatics (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Nutrition Science (AREA)
- Virology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
본 발명은 동과피 추출물을 유효성분으로 함유하는 선천면역 증진 및 항바이러스용 약학 조성물, 동과피 추출물을 유효성분으로 함유하는 선천면역 증진 및 항바이러스용 건강기능식품, 동과피 추출물을 유효성분으로 함유하는 선천면역 증진 및 항바이러스용 사료 조성물 및 동과피 추출물을 인간을 제외한 선천면역 증진이 필요한 동물에 투여하는 것을 포함하는 동물의 선천면역 증진 및 항바이러스 활성 증진 방법에 관한 것이다. 본 발명에 따른 선천면역 증진 및 항바이러스용 동과피 조성물은 항바이러스 활성 및 선천면역 증진 효능을 나타내어, 박테리아 및 바이러스 감염성 질환의 예방 및 치료용으로 적용될 수 있고, 면역이 저하되거나 면역이 억제된 환자의 면역력 증진 및 조절을 위한 의약이나 건강기능식품, 및 동물의 항 질병 강화를 목적으로 하는 선천면역 증진 및 항바이러스용 사료 등으로 광범위하게 이용될 수 있다. 또한, 본 발명에 따른 선천면역 증진 및 항바이러스용 조성물은 독성이나 부작용을 거의 일으키지 않으므로 예방 목적으로 장기간 복용시에도 안심하고 사용할 수 있다.The present invention is a pharmaceutical composition for enhancing innate immunity and antiviral containing a copper persimmon extract as an active ingredient, a health functional food for enhancing innate immunity and antiviral containing a copper perilla extract as an active ingredient, and a copper persimmon extract as an active ingredient It relates to a method for enhancing innate immunity and antiviral activity in animals, comprising administering a feed composition for enhancing innate immunity and antiviral, and an extract of the ginseng persimmon to animals in need of enhancement of innate immunity, except for humans. The sinus perilla composition for enhancing innate immunity and antiviral according to the present invention exhibits antiviral activity and innate immunity enhancement efficacy, and thus can be applied for the prevention and treatment of bacterial and viral infectious diseases, and patients with reduced immunity or suppressed immunity It can be widely used as medicine or health functional food for enhancing and controlling immunity, and as feed for innate immunity and antiviral for the purpose of strengthening animal anti-disease. In addition, since the composition for enhancing innate immunity and antiviral according to the present invention hardly causes toxicity or side effects, it can be safely used even when taken for a long period of time for the purpose of prevention.
Description
본 발명은 동과피 추출물을 유효성분으로 함유하는 선천면역 증진 및 항바이러스 조성물에 관한 것으로, 더욱 상세하게는 약학, 건강기능식품 또는 사료 조성물로 이용될 수 있는 동과피 추출물을 유효성분으로 함유하는 선천면역 증진 및 항바이러스 조성물에 관한 것이다.The present invention relates to an innate immunity enhancement and antiviral composition containing a copper persimmon extract as an active ingredient, and more particularly, to a congenital containing copper perilla extract as an active ingredient, which can be used as a pharmaceutical, health functional food or feed composition. It relates to immune enhancing and antiviral compositions.
면역은 태어날 때부터 지니고 있는 선천 면역(innate immunity)과 후천적으로 생활하면서 적응되어 얻어지는 획득 면역(acquired immunity)으로 구분될 수 있다. 선천 면역은 일명 '자연 면역'이라고도 하며, 항원에 대해 비특이적으로 반응하고, 특별한 기억작용은 없는 것을 특징으로 한다.Immunity can be divided into innate immunity, which is possessed from birth, and acquired immunity, which is acquired by living adaptively. Innate immunity, also called 'natural immunity', is characterized by non-specific responses to antigens and no special memory action.
최근 들어, 생체 내 선천적 방어 면역시스템 중에서 인터페론(interferon)에 의해 유도되는 선천성 면역분야가 주목을 받고 있는데, 인터페론 매개 면역의 활성화는 다양한 전염병 병원체에 대한 근본적인 예방 방법이 될 수 있기 때문이다. 이에, 인터페론 활성화 기전 연구 및 인터페론을 유도시킬 수 있는 면역조절제제의 개발 연구가 활발하게 진행되고 있다. Recently, the field of innate immunity induced by interferon among the innate defense immune system in vivo has been attracting attention, because the activation of interferon-mediated immunity can be a fundamental prevention method against various infectious disease pathogens. Accordingly, studies on the mechanism of interferon activation and development of immunomodulatory agents capable of inducing interferon are being actively conducted.
한편, 병원체의 감염에 따른 선천 면역의 방어 기작의 하나로 사이토카인 같은 면역 인자가 분비되는데, 이들에 의해 면역 반응이 일어나고 병원체에 대한 방어가 이루어진다. 따라서 선천 면역 반응을 유도함으로써 다양한 전염병 병원체 대한 예방 및 치료 방법이 될 수 있으며, 이를 유도시킬 수 있는 선천면역의 증진 제제에 대한 연구가 필요하다.On the other hand, immune factors such as cytokines are secreted as one of the defense mechanisms of innate immunity according to the infection of the pathogen, whereby an immune response occurs and defense against the pathogen is made. Therefore, it can be a preventive and therapeutic method for various infectious disease pathogens by inducing an innate immune response, and research on agents for enhancing innate immunity that can induce it is needed.
바이러스(virus)는 라틴어로 독성물질을 의미하며, 세균여과지(0.22㎛)를 통과하는 일군의 감염형 병원성 입자이다. 바이러스는 숙주세포의 종류에 따라 박테리오파지, 식물 바이러스, 동물 바이러스로 분류하기도 하며, 핵산의 종류에 따라 DNA 바이러스, RNA 바이러스로 분류할 수 있다. 최근 신종 플루, AI 및 구제역 등 다양한 바이러스 질병이 사회적으로 큰 문제를 일으켰으며, 이에 따라 바이러스 질병의 효과적인 대책에 대한 고민이 사회적으로 큰 관심을 불러일으키고 있다. Virus means a toxic substance in Latin, and is a group of infectious pathogenic particles that pass through a bacterial filter paper (0.22㎛). Viruses can be classified into bacteriophages, plant viruses, and animal viruses according to the type of host cell, and can be classified into DNA viruses and RNA viruses according to the types of nucleic acids. Recently, various viral diseases such as H1N1 influenza, AI and foot-and-mouth disease have caused great social problems, and accordingly, concerns about effective countermeasures for viral diseases are arousing great social interest.
현재 바이러스성 질병을 예방하기 위한 가장 좋은 방법은 백신접종이지만, 바이러스에 의한 질병의 경우, 대체로 많은 바이러스 혈청형(아형) 생성 등에 따른 백신의 효율성 문제가 중요하게 제기되고 있다. 이러한 백신의 문제점을 보완해 줄 수 있는 바이러스 예방용 억제제의 개발 및 보급은 중요한 사항이며, 이를 위해 특히 바이러스에 대한 초기 방어시스템인 생체 내 선천적 면역시스템을 자극하여 개체 동물의 면역력을 높여주는 예방제제의 발굴 및 개발은 중요한 제제 개발 방법이 될 수 있다.Currently, the best way to prevent viral diseases is vaccination, but in the case of diseases caused by viruses, the effectiveness of vaccines due to the generation of many viral serotypes (subtypes) is being raised as an important issue. The development and dissemination of inhibitors for virus prevention that can supplement the problems of these vaccines are important. The discovery and development of drugs can be an important drug development method.
인플루엔자바이러스의 증식을 억제하는 대표적인 항바이러스 제제로는 아만타딘(amantadine)과 리만타딘(rimantadine)이 있으나, 이들 두 가지 항바이러스 제제들은 혈청형 A형 인플루엔자바이러스에만 효과적이며, M2 단백질이 없는 혈청형 B형 인플루엔자바이러스에는 효과가 없는 것으로 확인되었다. 또한, 아만타딘과 리만타딘은 사용 시 인플루엔자바이러스 M2 단백질의 이온채널기능에 영향을 미치지 못하는 변이 바이러스의 출현이 매우 쉽게 일어나는 단점이 있는 것으로 확인되고 있다. 이러한 단점을 보완하기 위하여 16종의 모든 혈청형 A형 인플루엔자바이러스와 혈청형 B형 인플루엔자바이러스에 효과적인 항바이러스 제제로 자나미비르(zanamivir)와 오셀타미비르(oseltamivir)가 개발되었다. 그러나 자나미비르는 흡입 및 정맥 투여해야 하는 단점이 있으며, 오셀타미비르는 경구투여가 가능하나 최근 내성 바이러스의 출현 보고와 경구투여 시 구토와 현기증 등의 부작용이 있어 단점으로 지적되고 있다.Representative antiviral agents that inhibit the proliferation of influenza virus include amantadine and rimantadine, but these two antiviral agents are effective only for serotype A influenza virus, and serotype B without M2 protein It was confirmed that it had no effect on the influenza virus type. In addition, it has been confirmed that amantadine and rimantadine have a disadvantage in that the appearance of a mutant virus that does not affect the ion channel function of the influenza virus M2 protein occurs very easily when used. To compensate for these shortcomings, zanamivir and oseltamivir have been developed as effective antiviral agents against all 16 serotype A influenza viruses and serotype B influenza viruses. However, zanamivir has the disadvantage of having to be administered by inhalation and intravenous administration, and although oseltamivir can be administered orally, recent reports of the emergence of resistant viruses and side effects such as vomiting and dizziness when administered orally have been pointed out as disadvantages.
또한, 수포성구내염바이러스(Vesicular stomatitis virus)의 주된 통제 방법은 질병의 완전 치료가 불가능하기 때문에, 구제역과 마찬가지로 예방 및 차단 방역과 발생 지역 내 감수성 가축의 박멸이 최선의 방법이다.In addition, since the main control method for vesicular stomatitis virus is that it is impossible to completely cure the disease, the best method is prevention, blocking, quarantine and eradication of susceptible livestock in the area where it occurs, like foot-and-mouth disease.
또한, 뉴캐슬병 바이러스에 대한 백신은 크게 생독 백신과 사독 백신으로 구분되는데, 가장 널리 이용되어온 대표적인 뉴캐슬병 생독 백신주인 B1주와 La Sota주(Clone주 포함)는 백신 접종 반응을 유발하는 것으로 알려져 있으며, 전 세계적으로 사용되고 있는 뉴캐슬병 사독 백신인 다가 혼합 사독 오일 백신은 1회 백신 접종으로 3종 이상의 질병이 동시에 예방될 수 있으나, 산란계 농장의 경우 면역력 저하로 인한 뉴캐슬병 발생 피해 사례가 늘어나고 있다. In addition, vaccines against Newcastle disease virus are largely divided into live venom vaccine and dead venom vaccine, and B1 and La Sota (including Clone), which are the most widely used representative Newcastle disease live venom vaccines, are known to induce a vaccination reaction. The multivalent mixed dead poison oil vaccine, which is a worldwide Newcastle disease dead poison vaccine, can prevent three or more diseases at the same time with a single vaccination.
또한, RNA 바이러스 가운데에서도 비교적 크기가 작은 바이러스들이 있다. 이들은 작다는 뜻의 'pico'라는 말과 'RNA'를 합쳐 피코나바이러스라고 부르며, 여기에 속한 바이러스들을 통틀어 피코나바이러스과 (Picona viridae)라고 부른다. 피코나바이러스 과에 속하는 엔테로바이러스는 무균성 수막염, 수족구병, 포진성 구협염, 확장성 심근염, 급성 출혈성 결막염 등의 다양한 임상증상을 일으키는 약 70가지 혈청형을 포함하고 있으며, 폴리오바이러스(Poliovirus), 콕사키바이러스(Cossackie virus) 및 에코바이러스(Echo virus)와 기타 엔테로바이러스로 분류된다. 엔테로바이러스의 직경이 20~30nm 정도이며, 단일 가닥의 RNA를 유전자로 가지고 있다. 대부분이 등뼈동물의 소화기관을 비롯하여 호흡기관 및 중추신경계까지 감염되지만 뚜렷한 증상을 나타내지 않는 경우가 많다. 콕사키바이러스(Coxsackie virus, CXV)는 피코나바이러스 과에 속하는 인간 엔테로바이러스(human enterovirus)로서, 크게 A형과 B형으로 구분된다(Pallansch MA and Roos RP, Fields Virology, 4th edi, pp723-775, 2001). Also, among RNA viruses, there are viruses that are relatively small in size. They are called piconaviruses by combining the word 'pico' meaning small and 'RNA', and the viruses belonging to this group are collectively called the piconavirus family (Picona viridae). Enteroviruses belonging to the piconavirus family include about 70 serotypes that cause various clinical symptoms such as aseptic meningitis, hand, foot and mouth disease, herpetic stomatitis, dilated myocarditis, and acute hemorrhagic conjunctivitis. , Cossackie virus, Echo virus, and other enteroviruses. Enterovirus has a diameter of about 20-30 nm and has single-stranded RNA as its gene. Most of them infect the digestive system of vertebrates as well as the respiratory tract and the central nervous system, but in many cases they do not show any obvious symptoms. Coxsackie virus (CXV) is a human enterovirus belonging to the piconavirus family, and is largely divided into types A and B (Pallansch MA and Roos RP, Fields Virology, 4th edi, pp723-775). , 2001).
또한, 최근 세계 곳곳에서 고 위험성 엔테로바이러스 및 변종 바이러스(엔테로바이러스 71형(EV-71), 콕사키바이러스 A24 변이주)가 새롭게 발견되어 유행되고 있어 이를 조기에 탐지하기 위한 국가 간 공동감시체계 구축이 요구되고 있다. In addition, recently high-risk enteroviruses and mutant viruses (enterovirus type 71 (EV-71), coxsackie virus A24 mutant) have been newly discovered and prevalent all over the world, so the establishment of a joint monitoring system between countries to detect them at an early stage is essential. is being demanded
콕사키바이러스를 포함하는 엔테로바이러스는 척추동물의 소화기관을 비롯하여 호흡기관 및 중추신경계까지 감염되어 다양한 임상증상을 유발하기 때문에 국가 차원의 대책 마련이 시급히 요구되고 있으나 바이러스의 종류와 혈청형이 매우 다양하여 효과적인 상용화 백신이나 치료제가 개발되어 있지 못한 실정이다.Enteroviruses, including coxsackie virus, infect the digestive, respiratory and central nervous systems of vertebrates and cause various clinical symptoms. Therefore, an effective commercial vaccine or therapeutic agent has not been developed.
또한, 단순포진바이러스(Herpes Simplex Virus; 이하 HSV)는 헤르페스 바이러스 속에 속하는 DNA 바이러스로서 약 175nm의 크기를 가지는 비교적 큰 바이러스이며, 인간에게 널리 퍼져 있는 감염체이다. 단순포진 감염은 HSV 1형(이하, 'HSV-1'라 함) 및 HSV 2형(이하, 'HSV-2'라 함)의 감염으로써, 점막이나 피부를 침범하는 급성 수포성 질환이며 아토피가 있는 사람에게서 흔히 볼 수 있는 감염질환이다. HSV-1은 주로 입과 안구 주위에, HSV-2는 성기주위에 수포를 형성시킨다. 이러한 HSV의 감염은 면역기능이 저하되어 있는 환자들에게는 그 정도가 심하게 진행되며 경부암의 원인으로도 작용하는 것으로 보고되어 있다(Melnick, J. L., Adam, E. and Rawls, W., Concer, 34, 1355-1385, 1974). 또한, 신생아나 태아의 경우에는 스스로 HSV 항체를 생성하지 못할 뿐 아니라 모체의 항체가 태아에게 넘어가지 못하기 때문에, 일단 감염되면 대부분 치명적인 결과를 초래하게 된다고 알려져 있다. 현재, HSV-1에 의한 안구질환의 경우는 미국 내에서만 1년에 약 50만 명의 환자가 발생하며 이중 1000명 이상은 안구 이식수술을 받고 있는 것으로 알려져 있다. HSV-2의 경우 약 95%는 활동성 병변이 있는 상대방과의 성적 접촉을 통해 감염되는 것으로 알려져 있는데, 미국 성인 중 약 20~30%가 HSV-2에 감염되어 있으며, 이중 20 내지 50%는 재발성 성기 포진을 갖는 것으로 보고되어 있다(Johnson R. E. et al., N. Engl. J. Med., 321, 7,1989). In addition, Herpes Simplex Virus (hereinafter referred to as HSV) is a DNA virus belonging to the genus Herpes virus and is a relatively large virus having a size of about 175 nm, and is an infectious agent widely spread to humans. Herpes simplex infection is an infection of HSV type 1 (hereinafter referred to as 'HSV-1') and HSV type 2 (hereinafter referred to as 'HSV-2'). It is a common infectious disease in people with HSV-1 primarily causes blisters around the mouth and eyeballs, and HSV-2 around the genitals. It has been reported that such HSV infection progresses severely in patients with weakened immune function and acts as a cause of cervical cancer (Melnick, JL, Adam, E. and Rawls, W., Concer, 34, 1355-1385, 1974). In addition, in the case of a newborn or a fetus, it is known that most of the cases of HSV antibody cannot be generated by themselves, and that the mother's antibody cannot pass to the fetus, so that once infected, most of them lead to fatal results. Currently, in the case of eye disease caused by HSV-1, about 500,000 patients a year in the United States alone, and it is known that more than 1,000 of them are undergoing eye transplantation. It is known that about 95% of HSV-2 is transmitted through sexual contact with a partner with active lesions. About 20-30% of American adults are infected with HSV-2, of which 20-50% recur It has been reported to have genital herpes (Johnson RE et al., N. Engl. J. Med., 321, 7,1989).
HSV는 초기 감염 후에 피부나 점막 표피세포에서 복제를 시작하여 신경조직을 따라 이동한 후, 전 생애에 걸쳐 척추 신경절에 잠복하고 있다가 면역기능이 저하될 경우 재활성화되어 감염부위로부터 여러 가지 질환을 발생시키는 것으로 알려져 있다. HSV에 의한 감염질환에 대한 치료제로는 뉴클레오사이드(nucleoside) 유도체인 아시클로비르(Acyclovir)가 초기감염에 매우 효과적인 것으로 알려져 있으나(Bryson, Y. J. et al., N. Engl. J. Med., 308, 916, 1983), 이 약제의 효과를 유지하기 위해서는 약제를 계속해서 투여해야 하며, 투여가 중단될 경우 HSV에 의한 감염질환이 재발하는 것으로 알려져 있다(Mindel A. et al., Lancet i: 926-928(1988); Straus S.E. et al., N. Engl. J. Med., 310, 1545(1984)). 또한 HSV의 최초 감염에 대한 치료 후 재발하는 경우에는 치사율이 높다고 보고되어 있다(Nahmias, A. J. and Coleman, R. M., Immunobiology of Herpes Simplex Virus Infection CRC Press, Boca Raton, 92-102,1984).After initial infection, HSV begins to replicate in the skin or mucosal epithelial cells, moves along the nervous tissue, remains dormant in the spinal ganglion throughout life, and is reactivated when the immune function is lowered to prevent various diseases from the site of infection. known to cause As a treatment for infectious diseases caused by HSV, acyclovir, a nucleoside derivative, is known to be very effective in initial infection (Bryson, YJ et al., N. Engl. J. Med., 308, 916, 1983), in order to maintain the effect of this drug, it is necessary to continuously administer the drug, and it is known that when the administration is stopped, infectious diseases caused by HSV recur (Mindel A. et al., Lancet i: 926-928 (1988); Straus SE et al., N. Engl. J. Med., 310, 1545 (1984)). In addition, it has been reported that the mortality rate is high in the case of recurrence after treatment for the initial infection of HSV (Nahmias, A. J. and Coleman, R. M., Immunobiology of Herpes Simplex Virus Infection CRC Press, Boca Raton, 92-102, 1984).
지금까지 이러한 HSV의 감염을 예방하기 위한 백신이 개발되어 왔으나, 바이러스 자체를 약화시켜 사용하는 생백신(live attenuated vaccine)은 HSV의 게놈이 종양발생(oncogenesis)에 직접 관여한다는 사실이 밝혀짐으로 인해서 그 사용이 금지되어 왔다(Cappel, R., Sprecher, S., De Cuyper, F. and De Braekeleer, J., J. Med. Virol., 16, 137-145,1985).Vaccines to prevent HSV infection have been developed so far, but live attenuated vaccines used by attenuating the virus itself have been found to be directly involved in the oncogenesis of the HSV genome. Its use has been banned (Cappel, R., Sprecher, S., De Cuyper, F. and De Braekeleer, J., J. Med. Virol., 16, 137-145, 1985).
이러한 상황하에서, 최근 해외 및 국내에서 기존의 항바이러스 제제의 단점을 극복하고자 하는 많은 노력들이 이루어지고 있으며, 그 중의 하나로 국내에서는 생약 추출물 및 식물 추출물을 대상으로 항바이러스 효능에 대한 연구가 진행중이기는 하지만, 아직까지는 미비한 실정이므로, 기존의 항바이러스 제제의 단점을 극복하여 독성 및 부작용이 거의 없이 우수한 선천면역 증진 효과 및 항바이러스 활성을 발휘할 수 있는 생약 추출물을 유효성분으로 하는 조성물의 개발이 필요한 실정이다.Under this circumstance, recent efforts to overcome the shortcomings of existing antiviral agents abroad and domestically have been made. , which is still incomplete, it is necessary to overcome the disadvantages of the existing antiviral agents and develop a composition using a herbal extract as an active ingredient that can exhibit excellent innate immunity enhancing effect and antiviral activity with almost no toxicity and side effects. .
한편, 동과피(Benincasae Pericarpium)는 박과의 동과(Benincasa hispida Cogniaux.:冬瓜)의 열매껍질을 말한다. 생김새는 안쪽으로 한쪽 또는 양쪽 말림을 한 통상 또는 쌍통상을 이루고 크기는 고르지 않으며 바깥 면은 매끈하고 광택이 나는 엷은 황색이나 황록색 또는 어두운 녹색을 띠는 가죽질이고 흰 가루(粉霜)로 덮여 있다. 안쪽은 엉성하고 거칠며 유관속이 보인다. 질은 물러서 쉽게 꺾어진다. 다른 이름으로 백과자(白瓜子), 동과인(冬瓜仁), 과자(瓜子), 과서(瓜犀), 과변(瓜辨), 수지(水芝), 지지(地芝) 등이 있다. 동과(冬瓜)란 겨울에 숙성되는 외라는 뜻이다. 일반적으로 1~3월에 심는데, 10월에 파종하면 봄에 심는 것보다 열매가 더욱 크고 좋다고 한다. 서리가 내린 후에는 껍질이 밀가루를 바른 듯이 하얗게 되고 씨앗도 흰색이 되어 백동과(白冬瓜)라고 했으며 씨앗은 백과자(白瓜子)라고 하였다. 이 약은 냄새가 거의 없으며 맛은 달고 성질은 약간 차다. On the other hand, copper peel (Benincasae Pericarpium) is a copper foil and with (Benincasa hispida Cogniaux:. refers to the peel of the fruit冬瓜). Appearance is cylindrical or double cylindrical with one or both sides rolled on the inside, and the size is not uniform. . The inside is sloppy and rough, and the ducts are visible. The vagina recedes and breaks easily. Other names include baekwaja (白瓜子), dongwain (冬瓜仁), sweets (瓜子), gwaseo (瓜犀), gwabyeon (瓜辨), resin (水芝), and jiji (地芝). Donggwa (冬瓜) means oysters that ripen in winter. In general, it is planted between January and March, but it is said that the fruit is bigger and better if sown in October than if planted in the spring. After frost, the skin turns white as if it had been coated with flour, and the seeds became white, and they were called baekdonggwa (白冬瓜), and the seeds were called baekja (白瓜子). This medicine has almost no odor and has a sweet taste and slightly cold in nature.
동과피는 이뇨, 전신부종, 해수, 천식, 해열, 더위 먹었을 때 사용하며, 약리작용으로 비신성수종(非腎性水腫)에 효과가 있음이 보고되었다. 하지만 아직까지는 동과피 추출물을 이용한 선천면역 증진 효과 및 항바이러스 활성에 관해 보고된 바 없다. Donggwapi is used for diuresis, systemic edema, seawater, asthma, antipyretic, and heat. However, there has been no report on the effect of enhancing innate immunity and antiviral activity using the extract of Donggwa peel.
본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명은 동과피 추출물이 선천 면역의 주요 세포인 대식 세포를 활성화시켜 다양한 바이러스의 증식을 억제할 수 있음을 확인함으로써 본 발명을 완성하였다. 또한, 본 발명은 독성 및 부작용이 거의 없어 장기간 안전성을 확보하면서, 각종 바이러스 또는 세균에 의한 감염성 질환의 예방 또는 치료용 약학 조성물, 건강기능식품 또는 가축 사료 조성물 등에 효과적으로 이용될 수 있는 동과피 추출물을 유효성분으로 함유하는 선천면역 증진 및 항바이러스 조성물 및 동물의 선천면역 증진 및 항바이러스 활성 증진 방법을 제공하는 것을 그 목적으로 한다.The present invention has been derived from the above needs, and the present invention has been completed by confirming that the extract of Dong persimmon extract can inhibit the proliferation of various viruses by activating macrophages, which are the main cells of innate immunity. In addition, the present invention provides a copper persimmon extract that can be effectively used in pharmaceutical compositions for the prevention or treatment of infectious diseases caused by various viruses or bacteria, health functional foods or livestock feed compositions, etc. while ensuring long-term safety due to almost no toxicity and side effects. An object of the present invention is to provide an innate immunity enhancement and antiviral composition containing as an active ingredient, and a method for enhancing animal innate immunity and antiviral activity.
상기 목적을 달성하기 위하여, 본 발명은 동과피 추출물을 유효성분으로 함유하는 선천면역 증진 및 항바이러스용 약학 조성물을 제공한다.In order to achieve the above object, the present invention provides a pharmaceutical composition for enhancing innate immunity and antiviral containing the extract of Donggwa peel as an active ingredient.
또한, 본 발명은 동과피 추출물을 유효성분으로 함유하는 선천면역 증진 및 항바이러스용 건강기능식품을 제공한다.In addition, the present invention provides a health functional food for enhancing innate immunity and antiviral containing the extract of Donggwa peel as an active ingredient.
또한, 본 발명은 동과피 추출물을 유효성분으로 함유하는 선천면역 증진 및 항바이러스용 사료 조성물을 제공한다.In addition, the present invention provides a feed composition for enhancing innate immunity and antiviral containing the extract of Donggwa peel as an active ingredient.
또한, 본 발명은 인간을 제외한 선천면역 증진이 필요한 동물에 동과피 추출물을 투여하는 것을 특징으로 하는 동물의 선천면역 증진 및 항바이러스 활성의 증진 방법을 제공한다. In addition, the present invention provides a method for enhancing innate immunity and antiviral activity in animals, characterized in that the extract is administered to an animal in need of enhancement of innate immunity, except for humans.
본 발명에 따르면 우수한 선천면역 증진 효능을 발휘하는 동과피 추출물을 이용함으로써 각종 바이러스 및 세균 감염성 질병의 예방 및 치료에 효과적으로 적용될 수 있으며, 면역력 증진에 기여할 수 있다.According to the present invention, it can be effectively applied to the prevention and treatment of various viral and bacterial infectious diseases, and can contribute to the enhancement of immunity, by using the extract of the copper persimmon which exhibits excellent innate immunity enhancement effect.
또한, 본 발명에 따른 동과피 추출물을 포함하는 선천면역 증진 및 항바이러스용 조성물은 독성이나 부작용을 거의 일으키지 않으므로 예방 목적으로 장기간 복용시에도 안심하고 사용할 수 있다. 따라서 본 발명에 따른 조성물은 박테리아 및 바이러스에 의한 감염성 질환의 예방 및 치료용으로 적용될 수 있고, 면역이 저하되거나 면역이 억제된 환자의 면역력 증진 및 조절을 위한 약학 조성물이나 건강기능식품, 및 동물의 항 질병 강화를 목적으로 하는 면역증진용 사료 조성물 등으로 광범위하게 이용될 수 있다.In addition, the composition for enhancing innate immunity and antiviral containing the extract according to the present invention does not cause toxicity or side effects, so it can be safely used for long-term use for preventive purposes. Therefore, the composition according to the present invention can be applied for the prevention and treatment of infectious diseases caused by bacteria and viruses, and pharmaceutical compositions or health functional foods for enhancing and controlling immunity of immunocompromised or immunosuppressed patients, and animal products. It can be widely used as a feed composition for enhancing immunity for the purpose of strengthening anti-disease.
또한, 본 발명에 따르면 동과피 추출물을 선천면역 증진에 필요한 동물에 투여함으로써 동물의 면역을 효과적으로 안전하게 증진시킬 수 있다.In addition, according to the present invention, it is possible to effectively and safely enhance the animal's immunity by administering the extract to the animal required for innate immunity enhancement.
도 1은 본 발명의 일 실시예에 따른 동과피 추출물의 인플루엔자바이러스(PR8-GFP virus)에 대한 항바이러스 활성분석 결과이다. Medium은 음성 대조군으로 아무것도 처리하지 않은 세포군; PR8-GFP는 바이러스 감염군; IFN-β/PR8-GFP는 양성대조군으로, PR8-GFP 바이러스 감염 및 1,000Units/㎖의 IFN-β 처리군; 동과피/PR8-GFP는 PR8-GFP 바이러스 감염 및 동과피 추출물 처리군이다.
도 2는 본 발명의 일 실시예에 따른 동과피 추출물의 수포성구내염바이러스(VSV-GFP virus)에 대한 항바이러스 활성분석 결과이다. Medium은 음성 대조군으로 아무것도 처리하지 않은 세포군; VSV-GFP는 바이러스 감염군; IFN-β/VSV-GFP는 양성대조군으로, VSV-GFP 바이러스 감염 및 1,000Units/㎖의 IFN-β 처리군; 동과피/VSV-GFP는 VSV-GFP 바이러스 감염 및 동과피 추출물 처리군이다.
도 3은 본 발명의 일 실시예에 따른 동과피 추출물의 단순포진바이러스(HSV-GFP virus)에 대한 항바이러스 활성분석 결과이다. Medium은 음성 대조군으로 아무것도 처리하지 않은 세포군; HSV-GFP는 바이러스 감염군; IFN-β/HSV-GFP는 양성대조군으로, HSV-GFP 바이러스 감염 및 1,000Units/㎖의 IFN-β 처리군; 동과피/HSV-GFP는 HSV-GFP 바이러스 감염 및 동과피 추출물 처리군이다.
도 4는 본 발명의 일 실시예에 따른 동과피 추출물의 뉴캐슬병바이러스(NDV-GFP virus)에 대한 항바이러스 활성분석 결과이다. Medium은 음성 대조군으로 아무것도 처리하지 않은 세포군; NDV-GFP는 바이러스 감염군; IFN-/NDV-GFP는 양성대조군으로, NDV-GFP 바이러스 감염 및 1,000Units/㎖의 IFN- 처리군; 동과피/NDV-GFP는 NDV-GFP 바이러스 감염 및 동과피 추출물 처리군이다.
도 5는 본 발명의 일 실시예에 따른 동과피 추출물의 엔테로바이러스(EV-71 virus)에 대한 항바이러스 활성분석 결과이다. Medium은 음성 대조군으로 아무것도 처리하지 않은 세포군; EV-71는 바이러스 감염군; IFN-/EV-71은 양성대조군으로, EV-71 바이러스 감염 및 1,000Units/㎖의 IFN- 처리군; 동과피/EV-71는 EV-71 바이러스 감염 및 동과피 추출물 처리군이다.
도 6은 본 발명의 실시예에 따른 동과피 추출물에 의한 전 염증성 사이토카인 유도 분석결과를 나타낸다.
도 7은 본 발명의 실시예에 따른 동과피 추출물에 의한 인플루엔자바이러스(H1N1 및 H5N2)의 감염 억제를 확인한 결과이다. H1N1 및 H5N2는 인플루엔자바이러스만 처리(1.0 MOI)된 군이고, Medium은 음성대조군으로 아무것도 처리되지 않은 MDCK 세포군이며, 동과피/H1N1 및 동과피/H5N2는 1㎍/㎖의 동과피 추출물과 인플루엔자바이러스(1.0 MOI)를 동시에 처리한 군이다.1 is a result of analysis of antiviral activity against influenza virus (PR8-GFP virus) of an extract of Donggwa peel according to an embodiment of the present invention. Medium is a negative control, untreated cell group; PR8-GFP is a virus-infected group; IFN-β/PR8-GFP was a positive control group, including PR8-GFP virus infection and IFN-β treatment at 1,000 Units/ml; The sinus perilla/PR8-GFP is the PR8-GFP virus-infected group and the perilla extract treated group.
2 is a result of analysis of antiviral activity against vesicular stomatitis virus (VSV-GFP virus) of the extract of the sinus persimmon according to an embodiment of the present invention. Medium is a negative control, untreated cell group; VSV-GFP is a virus-infected group; IFN-β/VSV-GFP was a positive control group, including VSV-GFP virus infection and 1,000 Units/ml IFN-β treatment group; Spinach peel/VSV-GFP is a group treated with VSV-GFP virus infection and sinus hull extract.
Figure 3 is a result of analysis of antiviral activity against herpes simplex virus (HSV-GFP virus) of the extract of the copper persimmon skin according to an embodiment of the present invention. Medium is a negative control, untreated cell group; HSV-GFP is a virus-infected group; As a positive control, IFN-β/HSV-GFP was infected with HSV-GFP virus and treated with IFN-β at 1,000 Units/ml; The sinus perilla/HSV-GFP is the HSV-GFP virus-infected group and the perilla extract treated group.
4 is a result of analysis of antiviral activity against Newcastle disease virus (NDV-GFP virus) of the extract of the copper persimmon skin according to an embodiment of the present invention. Medium is a negative control, untreated cell group; NDV-GFP is a virus-infected group; IFN-/NDV-GFP was positive control, NDV-GFP virus infection and IFN- treatment group of 1,000 Units / ㎖; The sinus perilla/NDV-GFP is the NDV-GFP virus-infected group and the perilla extract treated group.
5 is a result of analysis of antiviral activity against the enterovirus (EV-71 virus) of the extract of the copper persimmon skin according to an embodiment of the present invention. Medium is a negative control, untreated cell group; EV-71 is a virus-infected group; IFN-/EV-71 was a positive control group, including EV-71 virus infection and IFN-treated group at 1,000 Units/ml; Donggwaskin/EV-71 is a group treated with EV-71 virus-infected and Donggwa skin extract.
Figure 6 shows the results of pro-inflammatory cytokine induction analysis by the extract of the copper persimmon skin according to an embodiment of the present invention.
7 is a result confirming the inhibition of infection of the influenza virus (H1N1 and H5N2) by the extract of the copper persimmon skin according to an embodiment of the present invention. H1N1 and H5N2 are groups treated with only influenza virus (1.0 MOI), Medium is a negative control group of MDCK cells that are not treated with anything, and sinus hull / H1N1 and dong rind / H5N2 are 1 ㎍ / ㎖ of copper rind extract and influenza virus. (1.0 MOI) was treated simultaneously.
본 발명은 동과피 추출물을 유효성분으로 함유하는 선천면역 증진 및 항바이러스용 약학 조성물에 관한 것이다. The present invention relates to a pharmaceutical composition for enhancing innate immunity and antiviral containing the extract of the copper persimmon skin as an active ingredient.
상기 동과피 추출물은 물, 탄소수 1 내지 4의 알코올 중에서 선택된 1종 이상의 용매로 추출하는 것이 바람직하고, 더 바람직하게는 물, 메탄올, 에탄올, 또는 부탄올의 용매를 이용하여 추출하는 것이며, 더욱더 바람직하게는 물을 용매로 이용하여 열수 추출한 것이다. 상기 열수 추출은 1) 동과피 중량을 기준으로, 5 내지 30배의 증류수를 첨가하는 단계; 2) 100~130℃의 온도에서 2~5시간 동안 열수 추출하는 단계; 및 3) 상기 열수 추출물을 여과하는 단계;를 거쳐 수행하는 것이 바람직하지만 이에 한정하지 않는다.The extract of the copper persimmon skin is preferably extracted with one or more solvents selected from water and alcohols having 1 to 4 carbon atoms, more preferably extracted using a solvent of water, methanol, ethanol, or butanol, even more preferably is hot water extraction using water as a solvent. The hot water extraction comprises the steps of 1) adding distilled water 5 to 30 times the weight of the copper persimmon skin; 2) extracting hot water at a temperature of 100 to 130° C. for 2 to 5 hours; and 3) filtering the hot water extract; preferably, but not limited thereto.
또한, 상기 동과피 추출물은 추출처리에 의해 얻어지는 추출액, 추출액의 희석액 또는 농축액, 추출액을 건조하여 얻어지는 건조물, 또는 조정제물 또는 정제물 중 어느 하나를 포함하는 것으로 한다.In addition, the copper persimmon skin extract shall include any one of an extract obtained by extraction treatment, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or a crude product or a purified product.
본 발명의 동과피 추출물이 항바이러스 활성을 갖는 바이러스의 일례는 오르토믹소비리대(Orthomixoviridae), 랍도비리대(Rhabdoviridae), 파라믹소비리대(Paramixoviridae), 허피스비리대(Herpesviridae) 및 피코나비리대(Picornaviridae) 중에서 선택된 하나 이상의 바이러스이며, 바람직하게는 인플루엔자바이러스, 뉴캐슬병바이러스, 수포성구내염바이러스(Vesicular stomatitis virus), 콕사키바이러스(Cossackie virus), 엔테로바이러스 71형(Enterovirus-71), 단순포진바이러스(Herpes Simplex Virus), 리노바이러스(Rhinovirus), 호흡기세포융합바이러스(respiratory syncytial virus; RSV), 구제역바이러스(Foot and mouth disease virus), 콜로라도참진드기열바이러스, 레오바이러스, 인간면역 결핍 바이러스, B형 간염바이러스, C형 간염바이러스, 돼지열병바이러스, 소바이러스성설사바이러스(Bovine Viral Diarrhea Virus), 돼지생식기호흡기증후군바이러스(Porcine reproductive and respiratory syndrome virus), 돼지오제스키병바이러스, 로타바이러스, 파보바이러스, 돼지유행성설사바이러스(Porcine epidemic diarrhea virus) 등이 있으며, 더 바람직한 바이러스의 일례는 인플루엔자바이러스, 뉴캐슬병바이러스, 수포성구내염바이러스, 단순포진바이러스, 엔테로바이러스 71형, 리노바이러스(Rhinovirus), 호흡기세포융합바이러스(respiratory syncytial virus; RSV)이지만 이에 제한하지 않는다. 병원체의 감염 시 선천 면역의 방어 기작의 하나로 면역 인자가 분비되는데, 이들 면역 인자들(TNF-α, IL-6 및 IFN-β)의 분비가 병원체를 방어하므로, 적정한 수준의 사이토카인의 반응 유도는 다양한 전염병 병원체에 대한 예방 및 치료 방법이 될 수 있다. 특히 바이러스에 대한 면역력을 증강시킬 수 있는 것이다. An example of a virus in which the extract of the sinus persimmon of the present invention has antiviral activity is Orthomixoviridae, Rhabdoviridae, Paramixoviridae, Herpesviridae and piconaviridae It is one or more viruses selected from the group Picornaviridae, preferably influenza virus, Newcastle disease virus, Vesicular stomatitis virus, Cossackie virus, Enterovirus type 71 (Enterovirus-71), herpes simplex. Herpes Simplex Virus, Rhinovirus, respiratory syncytial virus (RSV), Foot and mouth disease virus, Colorado tick virus, reovirus, human immunodeficiency virus, B hepatitis virus, hepatitis C virus, swine fever virus, bovine viral diarrhea virus There is porcine epidemic diarrhea virus, etc., and more preferred examples of viruses include influenza virus, Newcastle disease virus, vesicular stomatitis virus, herpes simplex virus,
본 발명의 동과피 추출물은 면역 인자 유도능을 강하게 나타내며, 개체의 선천면역을 증가시켜 바이러스의 감염 및 증식을 억제하는 효과가 있을 뿐만 아니라, 세포 독성이나 부작용을 거의 나타내지 않으므로 장기간 복용시에도 안심하고 사용할 수 있는 것을 특징으로 한다. The extract of the present invention strongly exhibits immune factor inducing ability, increases the individual's innate immunity to suppress virus infection and proliferation, and exhibits almost no cytotoxicity or side effects. It is characterized in that it can be used.
본 발명의 선천면역 증진 및 항바이러스용 약학 조성물은 약학적으로 허용가능한 담체, 부형제 또는 희석제를 더 포함할 수 있다. 본 발명에 이용될 수 있는 약학적으로 허용가능한 담체, 부형제 또는 희석제는 본 발명의 효과를 해하지 않는 한 특별히 제한되지 않으며, 예를 들어 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제, 윤활제, 감미제, 방향제, 보존제 등을 포함할 수 있다. 약학적으로 허용 가능한 담체, 부형제 또는 희석제의 대표적인 예로는, 락토즈, 덱스트로스, 슈크로스, 솔비톨, 만니톨, 자일리톨, 말티톨, 전분, 젤라틴, 글리세린, 아카시아 고무, 알지네이트, 칼슘포스페이트, 칼슘카보네이트, 칼슘실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로즈, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트, 광물유, 프로필렌글리콜, 폴리에틸렌글리콜, 식물성 오일, 주사가능한 에스테르, 위텝솔, 마크로골, 트윈 61, 카카오지, 라우리지 등을 들 수 있다. 본 발명의 선천면역 증진 및 항바이러스용 약학 조성물은 정제, 환제, 산제, 과립제, 캡슐제, 현탁액, 에멀젼, 시럽제, 에어로졸, 외용제, 좌제 및 주사제로 이루어진 군으로부터 선택되는 형태일 수 있다. 약학 조성물의 제제화 방법은 기술분야에 공지된 통상의 방법에 따라 수행될 수 있으며, 특별히 제한되지 않는다. The pharmaceutical composition for enhancing innate immunity and antiviral of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent. Pharmaceutically acceptable carriers, excipients or diluents that can be used in the present invention are not particularly limited as long as they do not impair the effects of the present invention, and include, for example, fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweetening agents, flavoring agents, preservatives, and the like. Representative examples of pharmaceutically acceptable carriers, excipients or diluents include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, gum acacia, alginate, calcium phosphate, calcium carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil, injectable Ester, Witepsol, Macrogol, Tween 61, cacao butter, laurage, etc. are mentioned. The pharmaceutical composition for enhancing innate immunity and antiviral of the present invention may be in a form selected from the group consisting of tablets, pills, powders, granules, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, and injections. The method for formulating the pharmaceutical composition may be performed according to a conventional method known in the art, and is not particularly limited.
본 발명의 선천면역 증진 및 항바이러스용 약학 조성물은 경구 또는 비경구 투여될 수 있으며, 투여량은 투여 대상의 연령, 성별, 체중, 상태, 질병의 정도, 약물의 형태, 투여 경로 및 기간에 따라 적절히 선택될 수 있으나, 일반적으로 약 5~500㎎/㎏, 바람직하게는 약 100~250㎎/㎏을 1일 1~3회 투여할 수 있다.The pharmaceutical composition for enhancing innate immunity and antiviral of the present invention can be administered orally or parenterally, and the dosage depends on the age, sex, weight, condition, degree of disease, type of drug, administration route and period of the subject. Although it may be appropriately selected, in general, about 5 to 500 mg/kg, preferably about 100 to 250 mg/kg, may be administered 1 to 3 times a day.
본 발명의 선천면역 증진 및 항바이러스용 약학 조성물의 제제화 방법, 투여량, 투여 경로, 구성성분 등은 기술분야에 공지된 통상의 기술로부터 적절히 선택될 수 있음이 통상의 기술자에게 명백하다. It is clear to those skilled in the art that the formulation method, dosage, administration route, components, etc. of the pharmaceutical composition for enhancing innate immunity and antiviral of the present invention can be appropriately selected from conventional techniques known in the art.
본 발명의 선천면역 증진 및 항바이러스용 약학 조성물은 박테리아 감염성 질병 또는 바이러스 감염성 질병의 예방 및 치료에 이용될 수 있다. 본 발명의 선천면역 증진 및 항바이러스용 약학 조성물은 유효 성분으로 동과피 추출물 외에 다른 약학적 활성 성분을 함께 포함하거나, 또는 다른 유효 성분을 포함하는 약학 조성물과 혼합되어 이용될 수 있다. The pharmaceutical composition for enhancing innate immunity and antiviral of the present invention can be used for the prevention and treatment of bacterial or viral infectious diseases. The pharmaceutical composition for enhancing innate immunity and antiviral of the present invention may include other pharmaceutically active ingredients in addition to the copper persimmon extract as an active ingredient, or may be used in combination with a pharmaceutical composition including other active ingredients.
또한, 본 발명은 동과피 추출물을 포함하는 선천면역 증진 및 항바이러스용 건강기능식품에 관한 것이다. 본 발명의 선천면역 증진 및 항바이러스용 건강기능식품은 식품학적으로 허용가능한 식품 보조 첨가제를 더 포함할 수 있다. 본 발명에 이용될 수 있는 식품학적으로 허용가능한 식품 보조 첨가제는 포도당, 과당, 말토오스, 슈크로스, 덱스트린, 시클로덱스트린과 같은 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당 알코올과 같은 천연 탄수화물, 타우마틴, 스테비아 추출물 등의 천연 향미제, 사카린, 아스파르탐산 등의 합성 향미제, 착색제, 펙트산 또는 그의 염, 알긴산 또는 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산화제 등을 포함하나, 이에 제한되는 것은 아니다. 본 발명의 선천면역 증진 및 항바이러스용 건강기능식품은 분말, 과립, 정제, 캡슐, 캔디, 츄잉검, 젤리 및 음료로 이루어진 군으로부터 선택되는 형태일 수 있다. 선천면역 증진 및 항바이러스용 건강기능식품 중의 동과피 추출물의 함량은 식품의 형태, 풍미, 맛 등을 고려하여 적절하게 선택될 수 있으며, 예를 들어 건강기능식품 전체 중량에 대하여 0.01~30 중량%의 범위일 수 있다. 본 발명의 선천면역 증진 및 항바이러스용 건강기능식품의 형태, 조성 및 제조방법 등은 기술분야에 공지된 통상의 기술로부터 적절히 선택될 수 있음이 통상의 기술자에게 명백하다.In addition, the present invention relates to a health functional food for enhancing innate immunity and antiviral containing the extract of the copper persimmon skin. The health functional food for enhancing innate immunity and antiviral of the present invention may further include a food-logically acceptable food supplementary additive. The pharmaceutically acceptable food supplement additives that can be used in the present invention include sugars such as glucose, fructose, maltose, sucrose, dextrin, and cyclodextrin, and natural carbohydrates such as sugar alcohols such as xylitol, sorbitol and erythritol, thaumatin , natural flavoring agents such as stevia extract, synthetic flavoring agents such as saccharin and aspartamic acid, coloring agents, pectic acid or salts thereof, alginic acid or salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, alcohols, carbonation agents, and the like, but are not limited thereto. The health functional food for enhancing innate immunity and antiviral of the present invention may be in a form selected from the group consisting of powder, granule, tablet, capsule, candy, chewing gum, jelly and beverage. The content of the copper persimmon extract in the health functional food for enhancing innate immunity and antiviral can be appropriately selected in consideration of the form, flavor, taste, etc. of the food, for example, 0.01 to 30% by weight based on the total weight of the health functional food may be in the range of It is clear to those skilled in the art that the form, composition and manufacturing method of the health functional food for enhancing innate immunity and antiviral of the present invention can be appropriately selected from conventional techniques known in the art.
또한, 본 발명은 동과피 추출물을 포함하는 선천면역 증진 및 항바이러스용 사료 조성물에 관한 것이다. 선천면역 증진 및 항바이러스용 사료 조성물 중의 동과피 추출물의 함량은 급여 가축의 종, 주령, 체중, 및 사육 조건 등에 따라 적절히 선택될 수 있으며, 사료 조성물 전체 중량에 대하여 0.01~95중량%, 바람직하게는 0.1~80중량%의 비율일 수 있다. 본 발명의 선천면역 증진 및 항바이러스용 사료 조성물은 기술분야에 공지된 사료 제조방법에 따라 제조될 수 있으며, 예를 들어, 각종 사료 원료 또는 배합사료와 본 발명의 동과피 추출물을 혼합한 후, 추가적인 가공 공정, 예를 들어 펠렛 형태로의 성형 또는 과립 등의 형태로의 절단 단계 등을 더 수행함으로써 제조될 수 있다. 본 발명의 선천면역 증진 및 항바이러스용 사료 조성물의 구성성분, 조성, 제조방법, 급여방법 등은 기술분야에 공지된 통상의 기술로부터 적절히 선택될 수 있음이 통상의 기술자에게 명백하다. In addition, the present invention relates to a feed composition for enhancing innate immunity and antiviral containing the extract of Donggwa peel. The content of copper persimmon extract in the feed composition for enhancing innate immunity and antiviral may be appropriately selected according to the species, age, weight, and breeding conditions of the fed livestock, and 0.01 to 95% by weight, preferably based on the total weight of the feed composition may be in a proportion of 0.1 to 80% by weight. The feed composition for enhancing innate immunity and antiviral of the present invention can be prepared according to a feed manufacturing method known in the art, for example, after mixing various feed raw materials or compound feed with the copper persimmon extract of the present invention, It may be manufactured by further performing an additional processing process, for example, molding into pellets or cutting into granules or the like. It is clear to those skilled in the art that the components, composition, manufacturing method, feeding method, etc. of the feed composition for enhancing innate immunity and antiviral of the present invention can be appropriately selected from conventional techniques known in the art.
또한, 본 발명은 인간을 제외한 선천면역 증진이 필요한 동물에 동과피 추출물을 투여하는 것을 특징으로 하는 동물의 선천면역 증진 및 항바이러스 활성의 증진 방법에 관한 것이다. 본 발명의 동물의 선천면역 증진 및 항바이러스 활성 증진에 있어서, 동과피 추출물의 투여량, 투여경로, 투여 시기 등은 기술분야에 공지된 통상의 기술로부터 적절히 선택될 수 있음이 통상의 기술자에게 명백하다.
In addition, the present invention relates to a method for enhancing innate immunity and antiviral activity in animals, characterized in that the extract is administered to an animal in need of enhancement of innate immunity, except for humans. It is clear to those skilled in the art that in the enhancement of the animal's innate immunity and antiviral activity of the present invention, the dosage, route of administration, administration time, etc. of the copper persimmon extract can be appropriately selected from conventional techniques known in the art. do.
이하, 실시예를 이용하여 본 발명을 더욱 상세하게 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로 본 발명의 범위가 이들에 의해 제한되지 않는다는 것은 당해 기술분야에서 통상의 지식을 가진 자에게 있어 자명한 것이다.
Hereinafter, the present invention will be described in more detail using examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited thereto.
실시예Example 1. 동과피 추출물의 제조 1. Preparation of Donggwa Peel Extract
동과피 추출물은 동과피의 중량을 기준으로, 20배의 증류수를 동과피에 첨가하여 115℃에서 180분간 열수 추출하고, 0.45㎛로 1차 여과한 후, 0.22㎛로 2차 여과하여 침전물을 제거하였다. 이어서, pH를 7.0으로 조정하여 1.5㎖ Ep-튜브에 1㎖씩 분주하고 -20℃에서 저장하여 모든 분석에 사용하였다.
For the copper persimmon extract, 20 times distilled water was added to the perilla skin based on the weight of the perilla skin, hot water extraction was performed at 115° C. for 180 minutes, first filtered with 0.45 μm, and second filtration with 0.22 μm to remove the precipitate. did Then, the pH was adjusted to 7.0, and each 1 ml was dispensed into a 1.5 ml Ep-tube, and stored at -20°C to be used for all analyses.
실시예Example 2. 동과피 추출물의 항바이러스 활성 분석 2. Analysis of Antiviral Activity of Donggwa Peel Extract
인플루엔자바이러스(Influenza virus; PR8), 수포성구내염바이러스(Vesicular stomatitis virus), 단순포진바이러스(Herpes simplex virus; HSV), 뉴캐슬병바이러스(Newcastle disease virus) 및 엔테로바이러스 71형(Enterovirus-71; EV-71)에 대한 동과피 추출물의 항바이러스 활성 분석을 수행하였다.
Influenza virus (PR8), Vesicular stomatitis virus, Herpes simplex virus (HSV), Newcastle disease virus and Enterovirus type 71 (Enterovirus-71; EV-71) ) was analyzed for the antiviral activity of the extract of Donggwa peel extract.
(1) 항바이러스 활성 분석 방법(1) Antiviral activity assay method
인플루엔자바이러스(Influenza virus; PR8), 수포성구내염바이러스(Vesicular stomatitis virus; VSV), 단순포진바이러스(HSV-GFP) 및 뉴캐슬병바이러스(Newcastle disease virus) 를 마우스 대식세포주인 Raw 264.7 세포(8×105cell/well)에 감염시켜 분석하였고, 엔테로바이러스 71형(Enterovirus-71; EV-71)은 Hela 세포에 감염시켜 분석하였다.Influenza virus (Influenza virus; PR8), vesicular stomatitis virus (Vesicular stomatitis virus; VSV), a Raw 264.7 cells (8 × 10 5 to herpes simplex virus (HSV-GFP) and Newcastle disease virus (Newcastle disease virus) mouse macrophage cell line cell/well), and enterovirus 71 (Enterovirus-71; EV-71) was analyzed by infecting Hela cells.
12-웰 TC 플레이트에 세포를 배양한 후, 1% FBS가 첨가된 DMEM에 상기 실시예 1에서 제조한 1㎍/㎖의 동과피 추출물(pH 조정)을 각각 처리하였다. 음성 대조군은 1% FBS가 첨가된 DMEM만을 처리하였고, 양성 대조군(Positive control)은 마우스 IFN-β(500units/㎖)를 처리하였다. 동과피 추출물의 처리 12시간 후, PR8-GFP(MOI:1.0), VSV-GFP(MOI:1.0), HSV-GFP(MOI:3.0), NDV-GFP(MOI:3.0) 및 EV-71(MOI:1.0)를 각각 접종하였다. 접종하고 2시간 후 접종액을 제거하고, PBS로 3회 세척하고, 12 및 24시간 후, 바이러스 감염 정도를 확인하였다.
After culturing the cells in a 12-well TC plate, 1 μg/ml of the copper perilla extract (pH adjustment) prepared in Example 1 was treated in DMEM supplemented with 1% FBS, respectively. A negative control group was treated only with DMEM supplemented with 1% FBS, and a positive control group was treated with mouse IFN-β (500 units/ml). After 12 hours of treatment with the radish extract, PR8-GFP (MOI: 1.0), VSV-GFP (MOI: 1.0), HSV-GFP (MOI: 3.0), NDV-GFP (MOI: 3.0) and EV-71 (MOI) :1.0) were inoculated, respectively. After 2 hours of inoculation, the inoculum was removed, washed 3 times with PBS, and after 12 and 24 hours, the degree of virus infection was checked.
(2) 항바이러스 활성 분석결과(2) Antiviral activity assay result
1) 인플루엔자바이러스(1) Influenza virus ( PR8PR8 -- GFPGFP virusvirus )의 분석결과) analysis result
인플루엔자바이러스에 대한 항바이러스 활성 분석결과를 도 1에 개시하였다. 도 1(a)는 감염 12시간 후의 GFP(green fluorescent protein) 형광이미지를 나타내고, 도 1(b)는 감염 24시간 후의 GFP(green fluorescent protein) 형광이미지를 나타낸 것이다. 동과피 추출물로 처리한 결과, 인플루엔자바이러스의 감염률이 현저하게 떨어졌으며, 세포 생존율도 바이러스 감염군에 비해 상승한 것을 확인할 수 있었다(도 1).
The results of the analysis of antiviral activity against influenza virus are disclosed in FIG. 1 . Figure 1 (a) shows a GFP (green fluorescent protein)
2) 2) 수포성구내염바이러스(VSV-GFP virus)의of vesicular stomatitis virus (VSV-GFP virus) 분석결과 Analysis
수포성구내염바이러스에 대한 항바이러스 활성 분석 결과를 도 2에 개시하였다. 도 2(a)는 감염 12시간 후의 GFP(green fluorescent protein) 형광이미지를 나타내고, 도 2(b)는 감염 24시간 후의 GFP(green fluorescent protein) 형광이미지를 나타낸 것이다. 도 2로부터 확인할 수 있는 바와 같이, 동과피 추출물로 처리한 결과, 수포성구내염바이러스의 감염률이 현저하게 떨어진 것을 확인하였다.
The results of the analysis of antiviral activity against vesicular stomatitis virus are shown in FIG. 2 . Figure 2 (a) shows a GFP (green fluorescent protein)
3) 단순포진바이러스(3) Herpes simplex virus ( HSVHSV -- GFPGFP virusvirus )의 분석결과) analysis result
단순포진바이러스에 대한 항바이러스 활성 분석결과를 도 3에 개시하였다. 도 3(a)는 감염 12시간 후의 GFP 형광이미지를 나타내고, 도 3(b)는 감염 24시간 후의 GFP 형광이미지를 나타낸 것이다. 도 3으로부터 확인할 수 있는 바와 같이, 동과피 추출물로 처리한 결과, 단순포진바이러스의 감염률이 현저하게 떨어진 것을 확인하였다.
The results of the analysis of antiviral activity against herpes simplex virus are shown in FIG. 3 . Figure 3 (a) shows the
4) 뉴캐슬병바이러스(NDV-GFP virus)의 분석결과 4 ) Analysis result of Newcastle disease virus (NDV-GFP virus)
뉴캐슬병바이러스에 대한 항바이러스 활성 분석결과를 도 4에 개시하였다. 도 4(a)는 감염 24시간 후의 GFP 형광이미지를 나타내며, 도 4(b)는 상대적인 GFP 형광량을 나타낸 것이다. 도 4에 개시한 바와 같이, 뉴캐슬병바이러스가 감염된 세포에 동과피 추출물을 처리한 결과, 뉴캐슬병바이러스가 현저하게 감소한 것을 확인하였다.
The results of the antiviral activity assay for Newcastle disease virus are shown in FIG. 4 . Figure 4 (a) shows the
5) 엔테로바이러스 ( EV -71 virus ) 71형의 분석결과 5 ) Result of analysis of enterovirus ( EV- 71 virus) 71
엔테로바이러스 71형에 대한 항바이러스 활성 분석결과를 도 5에 개시하였다. 도 5(a)는 감염 12시간 후의 광학이미지를 나타내고, 도 5(b)는 감염 24시간 후의 광학이미지를 나타낸 것이다. 도 5에 개시한 바와 같이, 엔테로바이러스 71형이 감염된 세포에 동과피 추출물을 처리한 결과, 엔테로바이러스 71형이 현저하게 감소한 것을 확인하였다.
The results of the antiviral activity assay for
실시예Example 3. 마우스 대식 세포주를 이용한 3. Using mouse macrophage cell lines 동과피의sinusoidal 전 염증성 사이토카인 유도( Induction of pro-inflammatory cytokines ( propro -- inflammatoryinflammatory CytokineCytokines InductionInduction ) 분석) analyze
동과피 추출물의 면역인자 유도효과를 확인하기 위하여 전 염증성 사이토카인 유도 분석을 수행하였다.A pro-inflammatory cytokine induction assay was performed to confirm the immune factor-inducing effect of the sinus persimmon extract.
(1) 전 염증성 사이토카인 유도 분석방법(1) Pro-inflammatory cytokine induction analysis method
마우스 대식세포주인 Raw 264.7을 키워 분석에 사용하였다. 6-웰 TC 플레이트에 세포를 배양한 후, 1% FBS가 첨가된 DMEM에 상기에서 제조된 동과피 추출물(pH 조정)을 첨가하여 처리하였다. 음성 대조군은 1% FBS가 첨가된 DMEM만을 처리하였고, 양성 대조군은 바이러스, 암세포 등의 외부 물질에 반응하여 분비되는 사이토카인으로, 세포 내 항암 및 항바이러스 작용을 일으켜 면역반응을 유도하는 물질인 인터페론-β(IFN-β)를 1000Units/㎖ 처리하였다. 시료는 1㎍/㎖의 동과피 추출물을 처리하고 12시간 후와 24시간 후의 세포에 대하여 TNF-α, IL-6 및 IFN-β의 생성량(pg/㎖)을 ELISA로 측정하였다.
A mouse macrophage cell line, Raw 264.7, was grown and used for analysis. After culturing the cells in a 6-well TC plate, 1% FBS was added to DMEM for treatment by adding the extract (pH adjustment) prepared above. The negative control group was treated only with DMEM supplemented with 1% FBS, and the positive control group was a cytokine secreted in response to external substances such as viruses and cancer cells, interferon, a substance that induces an immune response by causing intracellular anticancer and antiviral action. -β (IFN-β) was treated with 1000 Units/ml. Samples were treated with 1 ㎍ / ㎖ of the extract of Donggwa peel, and the production amount (pg / ㎖) of TNF-α, IL-6 and IFN-β in
(2) 전 염증성 사이토카인 유도 분석결과(2) Pro-inflammatory cytokine induction analysis result
동과피 추출물에 의한 전 염증성 사이토카인 유도 분석결과를 도 6에 개시하였다. 종양괴사인자 알파(tumor necrosis factor-α; TNF-α)는 주로 활성화된 대식세포에 의해 분비되며, 가장 중요한 역할은 면역세포의 조절이다. 또한 바이러스 복제를 억제하는 능력이 있는 것으로 알려져 있다. 인터루킨 6(Interleukin 6; IL-6는 B-세포를 활성화시켜 항체생산을 증가시켜 항원특이적 면역반응을 촉진하는 중요한 사이토카인이다. 인터페론(Interferon; IFN)은 세포의 조절물질로서 그 기능이 아주 다양하다. 예를 들면, 바이러스로부터의 세포보호, 조직배양에서나 골수에서의 세포분열 억제, T세포의 작용 조절, 자연면역세포(NK세포)의 기능 항진을 유도하여 식균작용을 상승시키고, 특수 암세포의 분열 억제하는 것으로 알려져 있다. 도 6에 개시한 바와 같이, Raw 264.7 세포에서 동과피 추출물에 의해 전 염증성 사이토카인인 IL-6 및 IFN-β가 강하게 유도되는 것을 확인할 수 있다.
The results of analysis of pro-inflammatory cytokine induction by the extract of sinus pericarp are disclosed in FIG. 6 . Tumor necrosis factor alpha (tumor necrosis factor-α; TNF-α) is mainly secreted by activated macrophages, and the most important role is the regulation of immune cells. It is also known to have the ability to inhibit viral replication. Interleukin 6 (IL-6) is an important cytokine that promotes antigen-specific immune response by activating B-cells to increase antibody production. Interferon (IFN) is a cellular regulator, and its function is very For example, it increases phagocytosis by inducing cell protection from viruses, inhibition of cell division in tissue culture or bone marrow, regulation of T cells, and enhancement of natural immune cells (NK cells), and special cancer cells. As shown in Figure 6, it can be confirmed that the pro-inflammatory cytokines IL-6 and IFN-β are strongly induced by the sinus perilla extract in Raw 264.7 cells.
실시예Example 4. 동과피 추출물에 의한 4. By extract of Donggwa peel H1N1H1N1 및 and H5N2H5N2 바이러스 감염 억제 분석 Viral Infection Suppression Assay
(1) 분석방법(1) Analysis method
MDCK 세포주에 1㎍/㎖의 동과피 추출물과 1.0 MOI(multiplicity of infection) 바이러스를 동시에 처리하고 24시간 후, 세포독성을 측정하기 위하여 10㎕의 Ez-Cytox 시약을 처리하고 12시간 동안 배양하고 450nm에서 흡광도를 측정하였다.
MDCK cell line was simultaneously treated with 1 μg/ml of copper persimmon extract and 1.0 MOI (multiplicity of infection) virus, and 24 hours later, 10 μl of Ez-Cytox reagent was treated to measure cytotoxicity, followed by incubation for 12 hours and 450 nm absorbance was measured.
(2) 분석결과(2) Analysis result
동과피 추출물에 의한 H1N1 및 H5N2 바이러스 감염에 의한 세포 생존도를 분석한 결과, H1N1 및 H5N2 바이러스 감염에 의해 세포 생존률이 약 20%로 감소하였으며, 1㎍/㎖의 동과피 추출물과 1 MOI 바이러스를 동시에 처리한 군의 경우, 세포 생존률이 75~90%로 나타나, 바이러스 감염에 의한 세포 생존률의 감소를 막아주는 것을 확인하였다(도 7).
As a result of analyzing the cell viability due to H1N1 and H5N2 virus infection by the sinus hull extract, the cell viability was reduced to about 20% by H1N1 and H5N2 virus infection. In the case of the group treated at the same time, the cell viability was 75-90%, confirming that the decrease in cell viability due to virus infection was prevented ( FIG. 7 ).
제조예production example 1. 주사제 1. Injection
상기 실시예 1에서 제조한 동과피 추출물: 100㎎Donggwa peel extract prepared in Example 1: 100 mg
소듐 메타비설파이트: 3.0㎎Sodium metabisulfite: 3.0 mg
메틸파라벤: 0.8㎎Methylparaben: 0.8mg
프로필파라벤: 0.1㎎Propylparaben: 0.1mg
주사용 멸균 증류수: 적량Sterile distilled water for injection: appropriate amount
상기 성분을 혼합하고 통상의 방법으로 최종 부피가 2 ㎖이 되도록 제조하여, 앰플에 충전하고 멸균하여 주사제를 제조하였다.
The above ingredients were mixed and prepared to have a final volume of 2 ml by a conventional method, filled in an ampoule, and sterilized to prepare an injection.
제조예production example 2. 정제 2. Refining
상기 실시예 1에서 제조한 동과피 추출물: 200㎎Donggwa peel extract prepared in Example 1: 200 mg
감자 전분: 100㎎Potato Starch: 100mg
락토오스: 100㎎Lactose: 100mg
콜로이드성 규산: 16㎎Colloidal silicic acid: 16 mg
스테아린산 마그네슘: 적량Magnesium Stearate: Appropriate amount
통상의 정제 제조방법에 따라 상기 성분을 혼합하고 타정하고 정제를 제조하였다.
According to a conventional tablet manufacturing method, the above ingredients were mixed and compressed to prepare a tablet.
제조예production example 3. 3. 캡슐제capsules
상기 실시예 1에서 제조한 동과피 추출물: 100㎎Donggwa peel extract prepared in Example 1: 100 mg
유당: 50㎎Lactose: 50mg
전분: 50㎎Starch: 50 mg
탈크: 2㎎Talc: 2 mg
스테아린산 마그네슘: 적량Magnesium Stearate: Appropriate amount
통상의 캡슐 제조방법에 따라 상기 성분을 혼합하고 젤라틴 캡슐에 충전하여 캡슐제를 제조하였다.
According to a conventional capsule manufacturing method, the above ingredients were mixed and filled in a gelatin capsule to prepare a capsule.
제조예production example 4. 4. 환제pill
상기 실시예 1에서 제조한 동과피 추출물: 120㎎Donggwa peel extract prepared in Example 1 above: 120 mg
옥수수 전분: 100㎎Corn Starch: 100 mg
멸균 증류수: 적량Sterile Distilled Water: Appropriate amount
상기 성분을 혼합하고, 통상의 환제 제조방법에 따라 적절한 크기를 갖는 구형으로 제환하여 환제를 제조하였다.
The above ingredients were mixed, and the pills were prepared by pills in a spherical shape having an appropriate size according to a conventional pill manufacturing method.
제조예production example 5. 건강 기능 식품 5. Health Functional Foods
1) 건강 음료1) Healthy drinks
올리고당(2%), 액상과당(0.5%), 설탕(2%), 식염(0.5%), 물(75%) 등의 음료 재료에 상기 실시예 1에서 제조된 동과피 추출물을 적량 혼합하여, 살균함으로써 음료를 제조하였다. An appropriate amount of the copper persimmon extract prepared in Example 1 is mixed with beverage ingredients such as oligosaccharide (2%), fructose liquid (0.5%), sugar (2%), salt (0.5%), and water (75%), Beverages were prepared by sterilization.
2) 기능성 식품2) Functional food
상기 실시예 1에서 제조한 동과피 추출물을 각종 비타민 및 미네랄 함유 기능성 식품에 적량 혼합하여 동과피 추출물이 함유된 기능성 식품을 제조하였다.
An appropriate amount of the copper perilla extract prepared in Example 1 was mixed with various vitamins and minerals-containing functional food to prepare a functional food containing the copper perilla extract.
제조예production example 6. 사료 조성물 6. Feed composition
동물용 배합 사료에 상기 실시예 1에서 제조된 동과피 추출물을 적량 혼합하여, 사료 조성물을 제조한 후, 펠렛화 및 과립화하였다.An appropriate amount of the extract prepared in Example 1 was mixed with the compound feed for animals to prepare a feed composition, followed by pelletization and granulation.
Claims (13)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020150039180A KR102349860B1 (en) | 2015-03-20 | 2015-03-20 | Composition for enhancing innate immunity and antivirus comprising Benincasae Pericarpium extract as effective component |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1020150039180A KR102349860B1 (en) | 2015-03-20 | 2015-03-20 | Composition for enhancing innate immunity and antivirus comprising Benincasae Pericarpium extract as effective component |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20160112826A KR20160112826A (en) | 2016-09-28 |
KR102349860B1 true KR102349860B1 (en) | 2022-01-11 |
Family
ID=57102071
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020150039180A KR102349860B1 (en) | 2015-03-20 | 2015-03-20 | Composition for enhancing innate immunity and antivirus comprising Benincasae Pericarpium extract as effective component |
Country Status (1)
Country | Link |
---|---|
KR (1) | KR102349860B1 (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103768177A (en) * | 2014-01-23 | 2014-05-07 | 念丁芳 | Externally applied medicinal composition for treating liver disease intestinal endotoxemia |
CN104367876A (en) * | 2014-11-21 | 2015-02-25 | 邱宝花 | Oral care solution and preparation method thereof |
-
2015
- 2015-03-20 KR KR1020150039180A patent/KR102349860B1/en active IP Right Grant
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103768177A (en) * | 2014-01-23 | 2014-05-07 | 念丁芳 | Externally applied medicinal composition for treating liver disease intestinal endotoxemia |
CN104367876A (en) * | 2014-11-21 | 2015-02-25 | 邱宝花 | Oral care solution and preparation method thereof |
Also Published As
Publication number | Publication date |
---|---|
KR20160112826A (en) | 2016-09-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR101780975B1 (en) | Composition for enhancing innate immunity and antivirus comprising Pini Pollen extract as effective component | |
KR101951003B1 (en) | Composition for enhancing innate immunity and antivirus comprising Echinopsis Radix extract as effective component | |
KR101770573B1 (en) | Composition for enhancing innate immunity and antivirus comprising Schizonepetae Spica extract as effective component | |
KR101930778B1 (en) | Composition for antivirus comprising Ulmi Cortex extract as effective component | |
KR101951010B1 (en) | Composition for enhancing innate immunity and antivirus comprising Isatidis Folium extract as effective component | |
KR101725938B1 (en) | INNATE IMMUNE ENHANCING AND ANTIVIRAL COMPOSITION COMPRISING EXTRACT OF Coptis japonica (Thunb.) Makino | |
KR101762606B1 (en) | Composition for enhancing innate immunity and antivirus comprising Foeniculi Fructus extract as effective component | |
KR101874465B1 (en) | Composition for enhancing innate immunity and antivirus comprising Eupatorii Herba extract as effective component | |
KR101782847B1 (en) | Composition for enhancing innate immunity and antivirus comprising Hoveniae Semen Cum Fructus extract as effective component | |
KR101837445B1 (en) | Composition for enhancing innate immunity and antivirus comprising Dianthi Herba extract as effective component | |
KR101837448B1 (en) | Composition for enhancing innate immunity and antivirus comprising Piperis Longi Fructus extract as effective component | |
KR101951008B1 (en) | Composition for enhancing innate immunity and antivirus comprising Psoraleae Semen extract as effective component | |
KR101762608B1 (en) | Composition for enhancing innate immunity and antivirus comprising Hoveniae Semen Cum Fructus extract as effective component | |
KR102022062B1 (en) | Composition for enhancing innate immunity and antivirus comprising Chelidonii herba extract as effective component | |
KR102287638B1 (en) | Composition for enhancing innate immunity and antivirus comprising Euonymi Lignum Suberalatum extract as effective component | |
KR102287633B1 (en) | Composition for enhancing innate immunity and antivirus comprising Puerariae Flos extract as effective component | |
KR101791036B1 (en) | Composition for enhancing innate immunity and antivirus comprising Melandrii Herba extract as effective component | |
KR20160118741A (en) | Composition for enhancing innate immunity and antivirus comprising Mori Ramulus or Mori Radicis Cortex extract as effective component | |
KR102349860B1 (en) | Composition for enhancing innate immunity and antivirus comprising Benincasae Pericarpium extract as effective component | |
KR101677119B1 (en) | INNATE IMMUNE ENHANCING AND ANTIVIRAL COMPOSITION COMPRISING EXTRACT OF Angelicae Tenuissimae Radix | |
KR102307757B1 (en) | Composition for enhancing innate immunity and antivirus comprising Typhae pollen extract as effective component | |
KR20160104979A (en) | Composition for enhancing innate immunity and antivirus comprising Eriocauli Herba extract as effective component | |
KR101800443B1 (en) | Composition for enhancing innate immunity and antivirus comprising Asteris Radix extract as effective component | |
KR20160112840A (en) | Composition for antivirus comprising Trichosanthis Radix extract as effective component | |
KR101967921B1 (en) | Composition for enhancing innate immunity and antivirus comprising Drynaria Rhizome extract as effective component |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant |