KR102314879B1 - Drinking jelly composition comprising Psyllium husk - Google Patents

Abstract

The present invention relates to a drinkable jelly composition comprising Plantago ovata and carrageenan, providing a drinkable jelly which comprises dietary fiber to help smooth bowel movements.

Description

Drinking jelly composition comprising Psyllium　husk

The present invention relates to a drinking jelly composition comprising psyllium hulls.

Due to life stress, irregular meals, lack of fiber and water intake, and lack of exercise, modern people have many symptoms of constipation due to discomfort in the digestive system and reduced digestive ability. In addition, the number of people complaining of diseases such as metabolic diseases accompanied by constipation is gradually increasing, and among them, it has been reported that chronic constipation develops into various intestinal diseases including colorectal cancer. According to data from the National Health Insurance Corporation in 2016, the incidence of constipation was 1.4 times higher in women than in men, and higher in the elderly (170,000, 27.6%) in their 70s and older than in children under the age of 9 (159,000, 25.8%). reported to appear Currently, the prevalence of constipation in Korea is 16.5%, which is increasing in women and the elderly. This was found to be due to increased consumption of convenience foods and high-calorie foods, decreased intake of dietary fiber such as vegetables and fruits, and dietary problems caused by excessive nutrition or nutritional imbalance.

As dietary fiber is known to be effective in preventing defecation, obesity, and geriatric diseases, consumers' preference for processed foods containing dietary fiber is increasing. There is no internationally standardized definition, and according to the research results so far, dietary fiber is 'a total of high molecular substances such as polysaccharides that are not broken down by human digestive enzymes' or 'plant ovaries that are not broken down or absorbed by human digestive enzymes' It can be defined as 'a pyrogenic polysaccharide'. Dietary fiber is composed of vegetable components such as mustach polysaccharide, resistant starch, resistance dextrin, inulin, linin, chitin, pectin, beta-glucan, and oligosaccharide, and has different properties depending on each component. have. Fermented dietary fiber is broken down by bacteria and microorganisms in the large intestine to produce metabolites of short-chain fatty acids that play a variety of roles in gut health.

Dietary fiber is generally classified into insoluble dietary fiber and soluble dietary fiber according to its solubility in water. When dietary fiber is ingested, it is excreted without being digested by the body, but only about 5% is excreted and the rest is used as food for E. coli and affects the function of the colon.

Soluble dietary fiber absorbs water and becomes a gelatinous, viscous substance that is either fermented or not fermented by bacteria in the digestive tract. Due to this, it can delay the emptying time of the stomach to keep the feeling of fullness, and physiologically active substances such as short-chain fatty acids can be easily fermented by bacteria in the large intestine. Soluble dietary fiber includes pectin contained in fruits, vegetable gum, and inulin contained in root vegetables (pork potatoes, etc.).

Insoluble dietary fiber reduces the internal pressure of the intestine, increasing the amount of stool, increasing the number of bowel movements, and shortening the passage time of the stool. According to several studies, fat and bile acids during digestion are not absorbed by the body but are adsorbed and excreted by insoluble dietary fiber, so it is reported to significantly reduce the amount of triglycerides and cholesterol in the blood. Also, due to the low pH formed of short-chain fatty acids, bile acids and fatty acids are excreted in a non-ionized form, which is known to lower the incidence of colorectal cancer because the degree of damage to the colonic mucosa is weak. Insoluble dietary fiber is mainly a component of plant cells and includes cellulose and lignin contained in whole grains, beans, and fruits.

An example of the present invention is to provide a composition comprising a psyllium shell and a gelling agent.

Another embodiment of the present invention is to provide a drinkable jelly containing psyllium hull which is organoleptically excellent while facilitating bowel function.

Hereinafter, the present invention will be described in more detail.

An example of the present invention relates to a drinking jelly composition comprising psyllium shells, a gelling agent, and water.

Psyllium husk (husk of plantain seed; Psyllium husk) included in the present invention contains insoluble and water-soluble dietary fiber, and contains a large amount of arabino-xylan-polysaccharide that forms mucus, and thus has excellent water retention. With these properties, it increases the water content and volume of the feces to help defecation, and has the effect of lowering serum cholesterol and LDL-cholesterol concentrations.

"Drinking jelly" according to the present invention is a gel-phase composition that can be drunk, and has appropriate hardness, spreadability, moisture content, viscosity, water yield, etc. may be to have Specifically, the drinking jelly composition according to an embodiment of the present invention may be a drinkable jelly that does not undergo a mechanical digestion process such as masticatory movement, or undergoes a minimum mechanical digestion process.

The drinking jelly composition according to an embodiment of the present invention is a homo-phase in which the entire composition is a single phase, and a plurality of jellies in the beverage are hetero-distributed in granular form. It may be distinct from -phase.

The drinking jelly composition according to an embodiment of the present invention may have one or more properties selected from the group consisting of the following (1) to (6):

(1) hardness of 100 to 1,500 (g),

(2) a viscosity of greater than 1,800 to less than 7,300 (mPa.s);

(3) pH is 2 to 5,

(4) Yield is 5 to 50 (%),

(5) spreadability 60 cm ² or less, and

(6) Water content of 50% by weight or more.

Since drinking jelly is a drinkable gel-phase composition, when psyllium hull powder is used as the main raw material, the moisture retention of the raw material itself increases, causing problems in the stirring and filling process during manufacture. For example, when manufacturing drinking jelly, a gel is formed using a gelling agent and then injected into a spout pouch at a high temperature. may occur. In addition, the one-time intake to satisfy the convenience of intake is limited, so there is a need for development of a manufacturing method for drinking jelly having an excellent sensory gel texture. In particular, the psyllium hull has a problem of absorbing a large amount of moisture and changing into a gel form, which is inconvenient due to excessively high viscosity when drinking. Due to the high viscosity, there was a limitation in mass production because it was necessary to stir the raw material at a high speed after inputting the raw material when manufacturing it in the form of a beverage. Accordingly, it was difficult to prepare the psyllium hull in the form of drinking jelly.

The psyllium hull applicable to the present invention may be a powder. The particle size of the psyllium hull powder is not particularly limited, and for example, 0.01 mm to 10 mm, 0.01 mm to 5 mm, 0.01 mm to 1 mm, 0.01 mm to 0.7 mm, 0.01 mm to 0.5 mm, 0.01 mm to 0.3 mm, 0.01 mm to 0.1 mm. mm, 0.05 mm to 10 mm, 0.05 mm to 5 mm, 0.05 mm to 1 mm, 0.05 mm to 0.7 mm, 0.05 mm to 0.5 mm, 0.05 mm to 0.3 mm, 0.05 mm to 0.1 mm, 0.1 mm to 10 mm, 0.1 mm to 5 mm , 0.1 mm to 1 mm, 0.1 mm to 0.7 mm, 0.1 mm to 0.5 mm, 0.1 mm to 0.3 mm, or may be a powder having an average particle diameter of 0.1 mm to 0.2 mm, but is not limited thereto.

The composition according to an embodiment of the present invention contains psyllium hulls in an amount of 0.1 to 10% by weight, 0.1 to 8% by weight, 0.1 to 5% by weight, 0.1 to 3% by weight, 0.1 to 2% by weight, 0.1 to 1.5% by weight, 0.5 to 10 % by weight, 0.5 to 8% by weight, 0.5 to 5% by weight, 0.5 to 3% by weight, 0.5 to 2% by weight, 0.5 to 1.5% by weight, 1 to 10% by weight, 1 to 8% by weight, 1 to 5% by weight , 1 to 3% by weight, 1 to 2% by weight, or 1 to 1.5% by weight may be included.

The gelling agent applicable to the present invention is from the group consisting of carrageenan, glucomannan, locust bean gum, xanthan gum, guar gum, agar, pectin, gelatin, gum arabic, gellan gum, alginic acid, konjac, cellulose, tara gum, and tamarind gum. It may include one selected kind, and preferably, the gelling agent may include carrageenan. For example, the gelling agent may include carrageenan and further include glucomannan, locust bean gum, or both. As an example, the carrageenan used as a gelling agent may be kappa carrageenan, but is not limited thereto.

According to an example of the present invention, the gelling agent is 0.01 to 5% by weight, 0.01 to 4% by weight, 0.01 to 3% by weight, 0.01 to 2% by weight, 0.01 to 1.5% by weight, 0.01 to 1% by weight, 0.05 to 5 wt%, 0.05-4 wt%, 0.05-3 wt%, 0.05-2 wt%, 0.05-1.5 wt%, 0.05-1 wt%, 0.1-5 wt%, 0.1-4 wt%, 0.1-3 wt% %, 0.1-2 wt%, 0.1-1.5 wt%, 0.1-1 wt%, 0.2-5 wt%, 0.2-4 wt%, 0.2-3 wt%, 0.2-2 wt%, 0.2-1.5 wt%, 0.2 to 1% by weight, 0.3 to 5% by weight, 0.3 to 4% by weight, 0.3 to 3% by weight, 0.3 to 2% by weight, 0.3 to 1.5% by weight, 0.3 to 1% by weight, 0.4 to 5% by weight, 0.4 to 4 wt%, 0.4-3 wt%, 0.4-2 wt%, 0.4-1.5 wt%, 0.4-1 wt%, 0.5-5 wt%, 0.5-4 wt%, 0.5-3 wt%, 0.5-2 wt% %, 0.5 to 1.5% by weight, or may be included in an amount of 0.5 to 1% by weight.

According to an example of the present invention, when the gelling agent includes carrageenan and glucomannan, the carrageenan and the glucomannan are 0.5:1 to 4:1, 0.5:1 to 3.5:1, 0.5:1 to 3:1, 0.5:1 to 2.5:1, 0.5:1 to 2:1, 0.5:1 to 1.5:1, 0.8:1 to 4:1, 0.8:1 to 3.5:1, 0.8:1 to 3:1, 0.8: 1 to 2.5:1, 0.8:1 to 2:1, 0.8:1 to 1.5:1, 1:1 to 4:1, 1:1 to 3.5:1, 1:1 to 3:1, 1:1 to It may be included in a weight ratio of 2.5:1, 1:1 to 2:1, or 1:1 to 1.5:1, and for example, may be included in a weight ratio of 1:1 to 1.5:1.

According to an example of the present invention, the gelling agent may include carrageenan, glucomannan, and locust bean gum. The weight ratio of the carrageenan and the glucomannan may be as described above. The glucomannan and the locust bean gum are 1:1 to 15:1, 1:1 to 12:1, 1:1 to 11:1, 1:1 to 10:1, 1:1 to 8:1, 1:1 to 7.5:1, 1:1 to 7:1, 2:1 to 15:1, 2:1 to 12:1, 2:1 to 11:1, 2:1 to 10:1, 2:1 to 8 :1, 2:1 to 7.5:1, 2:1 to 7:1, 3:1 to 15:1, 3:1 to 12:1, 3:1 to 11:1, 3:1 to 10:1 , 3:1 to 8:1, 3:1 to 7.5:1, 3:1 to 7:1, 4:1 to 15:1, 4:1 to 12:1, 4:1 to 11:1, 4 :1 to 10:1, 4:1 to 8:1, 4:1 to 7.5:1, 4:1 to 7:1, 5:1 to 15:1, 5:1 to 12:1, 5:1 to 11:1, 5:1 to 10:1, 5:1 to 8:1, 5:1 to 7.5:1, 5:1 to 7:1, 6:1 to 15:1, 6:1 to 12 :1, 6:1 to 11:1, 6:1 to 10:1, 6:1 to 8:1, 6:1 to 7.5:1, 6:1 to 7:1, 6.5:1 to 15:1 , 6.5:1 to 12:1, 6.5:1 to 11:1, 6.5:1 to 10:1, 6.5:1 to 8:1, 6.5:1 to 7.5:1, or 6.5:1 to 7:1 of It may be included in a weight ratio, for example, it may be included in a weight ratio of 4:1 to 10:1.

According to an embodiment of the present invention, the gelling agent includes carrageenan, glucomannan, and locust bean gum, and the carrageenan, the glucomannan, and the locust bean gum are 5 to 15: 2 to 12: 1, 8 to 12: 2 to 12: 1, 9 to 10: 2 to 12: 1, 5 to 15: 3 to 11: 1, 8 to 12: 3 to 11: 1, 9 to 10: 3 to 11: 1, 5 to 15: 4 to 10: 1, 8 to 12: 4 to 10: 1, 9 to 10: 4 to 10: 1, 5 to 15: 5 to 9: 1, 8 to 12: 5 to 9: 1, 9 to 10: 5 to 9: 1, 5 to 15: 6 to 8: 1, 8 to 12: 6 to 8: 1, or 9 to 10: may be included in a weight ratio of 6 to 8: 1, for example, 9 to 10: 6 to 8: 1 may be included in a weight ratio.

The composition according to an embodiment of the present invention may have a hardness sufficient to implement the form of drinking jelly, and specifically has a minimum hardness value of a level capable of maintaining the form of a gel phase, and is used for drinking. It can have less than the maximum possible hardness value. For example, the hardness of the composition according to an embodiment of the present invention is 100 to 1,500 (g), 100 to 1,300 (g), 100 to 1,200 (g), 100 to 1,100 (g), 100 to 1,000 (g), 100 to 900 (g), 100 to 850 (g), 100 to 800 (g), 100 to 700 (g), 100 to 600 (g), 100 to 500 (g), 150 to 1,500 (g), 150 to 1,300 (g), 150 to 1,200 (g), 150 to 1,100 (g), 150 to 1,000 (g), 150 to 900 (g), 150 to 850 (g), 150 to 800 (g), 150 to 700 (g), 150 to 600 (g), 150 to 500 (g), 300 to 1,500 (g), 300 to 1,300 (g), 300 to 1,200 (g), 300 to 1,100 (g), 300 to 1,000 (g), 300 to 900 (g), 300 to 850 (g), 300 to 800 (g), 300 to 700 (g), 300 to 600 (g), 300 to 500 (g), or 400 to 500 (g) may be, for example, may be 450 to 500 (g).

The composition according to an embodiment of the present invention may have a viscosity sufficient to implement the form of drinking jelly, and specifically, has a minimum viscosity value of at least a level capable of maintaining the form of a gel phase, and is drinkable. It can have up to the maximum viscosity value of this easy level. For example, the viscosity of a composition according to an embodiment of the present invention is greater than 1,800 to less than 7,300 (mPa.s), greater than 1,800 to 6,500 (mPa.s), greater than 1,800 to 6,000 (mPa.s), greater than 1,800 to 5,500 (mPa.s), greater than 1800 to 5,000 (mPa.s), greater than 1800 to 4,000 (mPa.s), greater than 1800 to 3,000 (mPa.s), greater than 1800 to 2,500 (mPa.s), greater than 1800 to 2,000 (mPa.s), 1,850 to less than 7,300 (mPa.s), 1,850 to 6,500 (mPa.s), 1,850 to 6,000 (mPa.s), 1,850 to 5,500 (mPa.s), 1,850 to 5,000 (mPa.s) ), 1,850 to 4,000 (mPa.s), 1,850 to 3,000 (mPa.s), 1,850 to 2,500 (mPa.s), or 1,850 to 2,000 (mPa.s).

The composition according to an embodiment of the present invention may have a spreadability enough to implement the form of drinking jelly, specifically, more than the minimum spreadability to the extent that it does not flow down when ingested by squeezing from a container filled with drinking jelly. and, when pressure is applied to a container filled with drinking jelly, it may have less than the maximum spreadability to be easily extruded. For example, the composition according to an embodiment of the present invention may have a spreadability of 90% or less, 80% or less, 70% or less, 60% or less, or 55% or less compared to a control that does not include carrageenan as a gelling agent. have. The spreadability may be an area spread when the same pressure is applied to a sample of the same volume at the same height. ^{For example, the composition according to an exemplary embodiment of the present invention has a spreading area (cm 2} ) when squeezed by applying the same pressure to a sample having a volume of about 5 ml at a height of about 15 cm, that is, the spreadability, 60 cm ² or less, 50 cm ² or less, 40 cm ² or less, or 35 cm ² or less. At this time, the ^{lower limit of the spreading area (cm 2} ) is 10 cm ² or more, 15 cm ² or more, 20 cm ² or more, 25 cm ² or more, or 30 cm ² or more. For example, the spreading area, that is, the spreadability of the composition according to an embodiment of the present invention, may be ^{30 to 35 (cm 2 ).}

The composition according to an embodiment of the present invention may have a pH range suitable for use as drinking jelly, and may have a pH range suitable for maintaining the degree of gel formation and gel strength. For example, the pH of the composition according to an embodiment of the present invention is 2 to 5, 2 to 4, 2 to 3.5, 2.5 to 5, 2.5 to 4, 2.5 to 3.5, 3 to 5, 3 to 4, or 3 to It may be 3.5.

The composition according to an exemplary embodiment of the present invention may have a water yield suitable for use as drinking jelly, and may have a water yield suitable for maintaining the degree of gel formation and gel strength. For example, the yield (%) of the composition according to an embodiment of the present invention is 5 to 50 (%), 5 to 40 (%), 5 to 30 (%), 5 to 25 (%), 10 to 50 (%) ), 10-40 (%), 10-30 (%), 10-25 (%), 15-50 (%), 15-40 (%), 15-30 (%), 15-25 (%) , 20 to 50 (%), 20 to 40 (%), 20 to 30 (%), or 20 to 25 (%).

The composition according to an embodiment of the present invention may have a moisture content suitable for use as drinking jelly, for example, the moisture content of the composition according to an embodiment of the present invention is 50% by weight or more, 60% by weight or more, 70% by weight or more. % or more, 80% by weight or more, or 84% by weight or more.

The drinking jelly composition of the present invention may further include indigestible maltodextrin. Indigestible maltodextrin is one of the water-soluble dietary fibers. The roasted dextrin obtained by heating from corn starch is enzymatically decomposed with α-amylase (derived from Bacillus subtilis or Bacillus licheniformis) and amyloglucosidase (derived from Aspergillus niger), and indigestible components are fractionated from the purified dextrin. Thus, it is suitable for food, and its physiological properties include an effect on the bowel movement, an effect of inhibiting postprandial blood glucose rise, an effect of inhibiting postprandial triglyceride rise, and an effect of reducing body fat. In relation to the intestinal function, indigestible maltodextrin promotes colonic motility and functions to increase the frequency and amount of defecation.

The indigestible maltodextrin is 0.05 to 25% by weight, 0.05 to 20% by weight, 0.05 to 15% by weight, 0.05 to 10% by weight, 0.05 to 8% by weight, 0.05 to 5% by weight, 0.05 to 3% by weight, 0.05 to 2.5 wt%, 0.1-25 wt%, 0.1-20 wt%, 0.1-15 wt%, 0.1-10 wt%, 0.1-8 wt%, 0.1-5 wt%, 0.1-3 wt%, 0.1-2.5 wt% %, 0.5 to 25% by weight, 0.5 to 20% by weight, 0.5 to 15% by weight, 0.5 to 10% by weight, 0.5 to 8% by weight, 0.5 to 5% by weight, 0.5 to 3% by weight, 0.5 to 2.5% by weight, 1 to 25% by weight, 1 to 20% by weight, 1 to 15% by weight, 1 to 10% by weight, 1 to 8% by weight, 1 to 5% by weight, 1 to 3% by weight, 1 to 2.5% by weight, 1.5 to 25 wt%, 1.5-20 wt%, 1.5-15 wt%, 1.5-10 wt%, 1.5-8 wt%, 1.5-5 wt%, 1.5-3 wt%, 1.5-2.5 wt%, 2-25 wt% %, 2 to 20% by weight, 2 to 15% by weight, 2 to 10% by weight, 2 to 8% by weight, 2 to 5% by weight, 2 to 3% by weight, or 2 to 2.5% by weight. .

The composition according to an embodiment of the present invention includes psyllium hull and indigestible maltodextrin, and the psyllium hull and the indigestible maltodextrin are 1:0.5 to 1:5, 1:0.5 to 1:4, 1:0.5 to 1: 3, 1:0.5 to 1:2.5, 1:0.7 to 1:5, 1:0.7 to 1:4, 1:0.7 to 1:3, 1:0.7 to 1:2.5, 1:0.9 to 1:5, 1:0.9 to 1:4, 1:0.9 to 1:3, 1:0.9 to 1:2.5, 1:1 to 1:5, 1:1 to 1:4, 1:1 to 1:3, 1: 1 to 1:2.5, 1: 1.5 to 1:5, 1:1.5 to 1:4, 1:1.5 to 1:3, 1:1.5 to 1:2.5, 1: 2 to 1:5, 1:2 to It may be included in a weight ratio of 1:4, 1:2 to 1:3, or 1:2 to 1:2.5. For example, the composition according to an embodiment of the present invention may include psyllium hull and indigestible maltodextrin in a weight ratio of 1:0.7 to 1:2.5, 1:1.1 to 1:2.5, or 1:1.5 to 1:2.5. .

The composition according to an embodiment of the present invention further comprises one or more selected from the group consisting of gelation accelerators, sugars, sweeteners, vitamins (eg, vitamin C), organic acids, acidity regulators, concentrates, minerals, and fragrances. it could be

The gelation accelerator may include at least one cation selected from the group consisting of sodium, magnesium, potassium, and calcium or a salt thereof.

The sugars include glucose, fructose, fructose, lactose, maltose, isomaltose, turanose, trehalose, xylitol, sorbitol, maltitol, lactitol, inositol, erythritol, starch syrup, isomerized sugar, dextrin, cyclodextrin, pro It may include at least one selected from the group consisting of lacto-oligosaccharide, maltooligosaccharide, isomaltooligosaccharide, galactooligosaccharide, soybean oligosaccharide, and lactosucrose.

The sweetener may include at least one selected from the group consisting of sorbatin, enzyme-treated stevia, stevia extract, stevia glycosides (rebaudioside, etc.), glycyrrhizin, saccharin, sucralose, and aspartame. The sweetener may be a high-sweetness sweetener.

The high-sweetness sweetener may be included in an amount of 0.001 to 1% by weight, 0.001 to 0.5% by weight, 0.01 to 1% by weight, or 0.01 to 0.5% by weight based on 100% by weight of the composition.

The organic acid may include at least one selected from the group consisting of acetic acid, lactic acid, citric acid, tartaric acid, fumaric acid, succinic acid, malic acid, gluconic acid, adipic acid, and phytic acid.

The acidity regulator may include one or more selected from the group consisting of antioxidants, sodium acetate, sodium citrate, sodium malate, sodium tartrate, sodium succinate, and sodium fumarate.

The concentrate may be a fruit concentrate, for example, it may include a fruit extract. The fruit extract may include, for example, at least one selected from the group consisting of mango, banana, raspberry, strawberry, green grape, pineapple, peach, orange, lemon, citron, apple, grape, and lychee. The fruit extract may include a fruit juice extracted from fruit juice, a concentrate of the fruit juice, a filtrate of the concentrate, and the like.

The above minerals (electrolyte and trace elements) include magnesium chloride, sodium chloride, sodium acetate, magnesium sulfate, magnesium chloride, calcium chloride, dipotassium phosphate, monosodium phosphate, calcium glycerophosphate, sodium iron succinate citrate, manganese sulfate, copper sulfate. , zinc sulfate, sodium iodide, potassium sorbate, zinc, manganese, copper, iodine, and may include one or more selected from the group consisting of cobalt.

The minerals are 0.01 to 5% by weight, 0.01 to 3% by weight, 0.01 to 1% by weight, 0.1 to 5% by weight, 0.1 to 3% by weight, or 0.1 to 1% by weight based on 100% by weight of the composition. it could be

The flavoring may include flavorings for food, such as fruit flavor, flower flavor, mint flavor, vanilla flavor, cream flavor, chocolate flavor, or caramel flavor.

The composition according to an embodiment of the present invention may be provided in a packaged state in a container. For example, it may be prepared to form a gel of a specific strength by filling a container with the solution for manufacturing jelly for drinking. The container may be in the form of a pouch, cup, stick, or the like. Accordingly, the composition according to an embodiment of the present invention may be a pouch-type jelly (jelly pouch), a cup-type jelly (jelly cup), or a stick-type jelly (jelly stick). For example, the composition according to one embodiment of the present invention may not have a gummy shape. Specifically, the composition according to an embodiment of the present invention may have a weaker gel strength than gummy jelly, but maintain the gel form in the container.

The pouch-type jelly is provided in a form contained in a pouch-shaped container, and is a form of food that can be consumed by squeezing the jelly contained in the pouch by applying pressure.

The cup-shaped jelly (jelly cup) is provided in a form contained in a cup-shaped container, and is a form of food that can be ingested by scooping it with a spoon or the like.

The stick-type jelly is provided in a form contained in a container in the form of a stick, and is in the form of food that can be ingested by squeezing it by applying pressure.

Another example of the present invention relates to a composition for improving constipation comprising psyllium shells, a gelling agent, and water. The psyllium shell and the gelling agent are the same as described above.

The composition may be a composition for improving the reduced amount of defecation or for reducing residual stool in the large intestine.

The composition may induce at least one characteristic selected from the group consisting of the following (1) to (9):

(1) an increase in the amount (weight) of feces, specifically, an increase in the total weight of feces by an increase in the size of feces, for example, 1.5 times or more, 1.6 times or more, 1.7 times or more, or The rate of increase in the amount of feces more than 1.8 times,

(2) an increase in stool size, for example, a rate of increase of 3 times or more, 3.1 times or more, 3.2 times or more, or 3.3 times or more of the stool size increase rate (%) of the control group,

(3) increase in the water content of the stool, for example, 10% or more, 20% or more, 25% or more, 30% or more, 40% or more, or 45% or more increase rate of the water content, or the increase rate of the water content of the feces of the control group (%) ) 12 times or more, 13 times or more, 14 times or more, or 15 times or more of stool moisture content increase rate,

(4) a decrease in the weight of residual feces in the large intestine, for example, a decrease of 10% or more, 15% or more, or 20% or more of the residual fecal weight in the large intestine compared to the control;

(5) improvement of colon inflammation;

(6) improvement of villous monocyte infiltration;

(7) promote the secretion of mucus in the large intestine,

(8) promote the secretion of acidic mucus in the large intestine;

(9) promote the secretion of neutral mucus in the large intestine.

The present invention can provide a jelly composition for drinking psyllium hull which facilitates a bowel movement and has an excellent sensory gel texture.

1A is a view showing the state of gel formation of a sample using a gelling agent alone.
Figure 1b is a diagram showing the spreadability of the psyllium hull drinking jelly sample according to the type of gelling agent.
2 is a view showing the change in body weight of each experimental group according to an embodiment of the present invention.
3 is a view showing the amount of stool in each experimental group according to an embodiment of the present invention.
4 is a view showing the amount of stool increase (%) in each experimental group according to an embodiment of the present invention.
5 is a view showing the number of sides in each experimental group according to an embodiment of the present invention.
6 is a view showing the side shape of each experimental group according to an embodiment of the present invention.
7 is a view showing the moisture content before induction of constipation and after sample intake in each experimental group according to an embodiment of the present invention.
8 is a view showing an increase in the stool moisture content of each experimental group according to an embodiment of the present invention.
9 is a view showing the weight of the stool remaining in the large intestine of each experimental group according to an embodiment of the present invention.
10 is a view showing the results of H&E staining of colon tissues in each experimental group according to an embodiment of the present invention.
11a is a view showing the results of Periodic acid-Schiff staining and Alcian Blue staining in order to examine the generation, secretion state, and acidity of mucin, which is a component of intestinal mucus, in each experimental group according to an embodiment of the present invention.
11B is a view showing the results of confirming the mRNA expression level of interleukin 6 (IL-6) in the colon tissue in each experimental group according to an embodiment of the present invention.
12A is a view showing the wet weight (g) of the feces of each experimental group according to an embodiment of the present invention.
12B is a view showing an increase in wet weight (%) of the feces of each experimental group according to an embodiment of the present invention.
13 is a picture of the feces excreted for 24 hours after induction of constipation and after ingestion of the sample in each experimental group according to an embodiment of the present invention, and the area (size) of each feces was calculated through the Image J program.
14 is a view showing the results of measuring the dry weight of stools collected for 24 hours in each experimental group according to an embodiment of the present invention at 70° C. for 72 hours, and then calculating the moisture content of the stools and converting them into %. .
15A is a view showing photographs of the large intestine and cecum of each experimental group according to an embodiment of the present invention.
15B is a view showing the weight of stool remaining in the large intestine for each experimental group according to an embodiment of the present invention.
16 is a view showing the results of H&E staining of the large intestine and cecum for each experimental group according to an embodiment of the present invention.
17 is a view showing the results of PAS staining and AB staining of the large intestine and caecum for each experimental group according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

Example 1: Observation of physical properties of samples using gelling agent alone

To prepare a sample using the gelling agent alone, With the ingredients and contents shown in Table 1, sugar, a gelling agent, and psyllium hull powder are quantitatively added to purified water at a temperature of 70 to 85°C, and then completely dissolved by stirring. After stirring sufficiently, it was placed in a beaker and cooled in a refrigerator for at least 3 hours, and then the physical properties were observed. Sample 1 is xanthan gum as a gelling agent, Sample 2 is locust bean gum as a gelling agent, Sample 3 is guar gum as a gelling agent, Sample 4 is agar as a gelling agent, Sample 5 is gellan gum as a gelling agent, and Sample 6 is a gelling agent. In the case of kappa carrageenan and sample 7, glucomannan was used as a gelling agent. In Table 1, the content unit of each component is weight %.

division Purified water white sugar corn syrup gelling agent psyllium skin powder anhydrous citric acid vitamin C total sample 1 69.47 15.00 13.00 1.20 1.20 0.12 0.01 100.00 sample 2 69.56 15.00 13.00 1.20 1.20 0.03 0.01 100.00 sample 3 69.57 15.00 13.00 1.20 1.20 0.03 0.01 100.00 sample 4 69.57 15.00 13.00 1.20 1.20 0.02 0.01 100.00 sample 5 69.52 15.00 13.00 1.20 1.20 0.07 0.01 100.00 sample 6 69.57 15.00 13.00 1.20 1.20 0.02 0.01 100.00 sample 7 69.55 15.00 13.00 1.20 1.20 0.04 0.01 100.00

After the samples 1 to 7 were cooled in a refrigerator for 3 hours or more, the degree of gel formation was visually evaluated. The appearance of the gel formation of Samples 1 to 7 is shown in FIG. 1A, and it is described in Table 2 by observing whether the properties are maintained and spreadability.

division gelling agent maintenance spreadability sample 1 xanthan gum X - sample 2 locust bean gum X - sample 3 Guar Gum O X sample 4 agar O X sample 5 gellan gum O X sample 6 kappa carrageenan O X sample 7 glucomannan O X

As shown in Figure 1a and Table 2, when the gelling agent and the chamfering were applied together, a gel was formed, but there was a difference in the strength of the gel. It was not good, so it was difficult to implement the drinking jelly with the desired texture. Therefore, the gelling agent used in the above experiment was used alone or in a mixture of two or more when manufacturing the drinking jelly.

Example 2: Preparation of psyllium hull drinking jelly

Blue grape concentrate, fructose and gelling agent were quantitatively added to purified water at a temperature of 70 to 85° C. with the components and contents shown in Table 3 below, and then completely dissolved by stirring. Then, the following magnesium chloride, steviol glycoside, trisodium citrate, citric acid anhydride, sucralose, calcium lactate, DL-malic acid, vitamin C, indigestible maltodextrin, psyllium skin powder, and green grape flavor were added to the mixture and the remaining purified water was added. was added and sufficiently stirred. The obtained product was sterilized at a temperature of 90 to 95° C. for 1 to 5 minutes, filled in a spout pouch, and cooled at room temperature to prepare a drinking jelly for psyllium shell.

Each sample commonly contains 1.2% by weight of psyllium skin powder, 2.45% by weight of indigestible maltodextrin, 5.000% by weight of green grape concentrate, 0.300% by weight of green grape flavor, 5.000% by weight of fructose, 0.530% by weight of magnesium chloride, 0.010% by weight of steviol glycoside, Trisodium citrate 0.030% by weight, citric anhydride 0.210% by weight, sucralose 0.010% by weight, calcium lactate 0.190% by weight, DL-malic acid 0.090% by weight, and vitamin C 0.070% by weight were included. Glucomannan, and one or more selected from the group consisting of locust bean gum, were prepared by changing to two or more, or three kinds. Table 3 shows the main components and contents of each sample. In Table 3, the content unit of each component is weight %.

division psyllium skin powder Indigestible maltodextrin kappa carrageenan glucomannan Locust Bean Gum Purified water total sample 8 1.2 2.45 - - - 84.91 100 sample 9 1.2 2.45 0.28 - - 84.63 100 sample 10 1.2 2.45 - 0.21 - 84.7 100 sample 11 1.2 2.45 - - 0.03 84.88 100 sample 12 1.2 2.45 0.28 0.21 - 84.42 100 sample 13 1.2 2.45 0.28 - 0.03 84.6 100 sample 14 1.2 2.45 - 0.21 0.03 84.67 100 sample 15 1.2 2.45 0.28 0.21 0.03 84.39 100 sample 16 1.2 2.45 0.28 0.11 0.03 84.49 100 sample 17 1.2 2.45 0.28 0.32 0.03 84.28 100

Example 3. Evaluation of physical properties of psyllium hull drinking jelly

(1) pH measurement

Table 4 shows the pH of the psyllium hull drinking jelly of Samples 8 to 17 prepared in Example 2 using a pH meter. As shown in Table 4, the pH of the psyllium hull drinking jelly showed a value of about pH 3 to 4, and was in a pH range suitable for use as drinking jelly, and was suitable for maintaining the degree of gel formation and gel strength.

division pH brix Hardness Viscosity (mPa.s) Yield rate (%) Spreadability (cm ² ) sample 8 3.28 12.91 Measurable 1,500 26.4 72.29 sample 9 3.28 12.83 682.58 2,000 36.0 41.28 sample 10 3.28 12.94 Measurable 9,500 20.6 62.67 sample 11 3.27 12.77 Measurable 1,800 19.2 71.13 sample 12 3.28 13.38 470.03 2,800 24.8 31.42 sample 13 3.29 13.00 808.24 2,500 23.2 32.89 sample 14 3.26 12.84 Measurable 7,300 39.8 62.21 sample 15 3.29 13.26 475.28 1,900 23.8 33.10 sample 16 3.23 12.28 153.04 2,050 12.0 29.88 sample 17 3.23 12.25 1028.95 4,750 10.2 28.33

(2) Brix measurement

The Brix of the psyllium hull drinking jelly of Samples 8 to 17 prepared in Example 2 was measured using an ATAGO Digital Refractometer RX-5000-α and shown in Table 4. As shown in Table 4, the psyllium hull drinking jelly exhibited a similar Brix value of about 10 to 15 between samples.

(3) Hardness measurement

In order to measure the brittleness when the psyllium hull drinking jelly sample was compressed with a constant force, the hardness was measured using a texture analyzer. The psyllium hull drinking jelly samples of Samples 8 to 17 were left in the refrigerator for 3 hours or more, and then cut into cubes of width (10 mm), length (10 mm), and height (10 mm) and used as specimens. The hardness measurement conditions are shown in Table 5, and the hardness measurement results are shown in Table 4.

As shown in Table 4, sample 8, sample 10, sample 11, and sample 14 had too low gel strength to measure hardness. It was found that the sample containing carrageenan was preferable because it had appropriate hardness to be used as drinking jelly. The technical features are not limited to these theoretical inferences.

item condition probe Round, 35mm in diameter Pre-test speed 5mm/sec test speed 2mm/sec Post-test speed 2mm/sec Pressure 98% compression

(4) Viscosity measurement

For the psyllium hull drinking jelly sample prepared in Example 2, the viscosity was measured using a viscometer (Brookfield VT230) and shown in Table 4. The viscosity indicates the degree of stickiness of the sample, and in the case of Sample 15, the viscosity was lower than that of Sample 12 and Sample 13, so it was determined that drinking was easy.

(5) completion rate

For the psyllium hull drinking jelly samples prepared in Example 2, the yields were measured and shown in Table 4 after storage at 35° C. for 10 days. Specifically, a tissue was placed on a scale to measure the weight, and then a small amount of drinking jelly was squeezed on it and spread evenly, and then the weight was measured. After leaving the tissue for 30 minutes, after removing the jelly remaining on the tissue, the weight of the tissue was measured again, and the weight of the water released from the jelly was measured by subtracting the weight of the first measured tissue. The water yield was calculated as in Equation 1 below by dividing the weight of water released from the jelly by the weight of the first jelly.

[Equation 1]

Yield (%) = (weight of water released from jelly) / (weight of jelly) X 100 (%)

(6) spreadability measurement

The spreadability of the psyllium hull drinking jelly samples prepared in Example 2 was measured. Spreadability was measured as the ^{spread area (cm 2} ) after squeezing each sample in the same amount of about 5 ml at the same height of about 15 cm. The spreadability measurement results are shown in Table 4 and FIG. 1B. As shown in Table 4 and FIG. 1B, in the case of Sample 8, Sample 10, Sample 11, and Sample 14 that did not contain carrageenan, the spreadability was too high, and thus the retention of the jelly formulation was deteriorated.

Specifically, samples 9, 12, 13, 15, 16, and 17 of psyllium hull gel-like drinking jelly containing carrageenan are samples that do not contain carrageenan (eg, sample 8, sample 10, sample 11). , or sample 14) showed spreadability of 90% or less, maintaining the jelly formulation to maintain the shape, and had an excellent sensory effect when consumed in the form of a pouch.

Example 4. Sensory evaluation of psyllium hull drinking jelly

The sensory evaluation including texture satisfaction was performed on the psyllium hull drinking jelly samples prepared in Example 2. Specifically, hold the sample in the mouth, stimulate the epidermis of the oral cavity evenly for 20 seconds, spit it out, wash the mouth sufficiently with hot water after each evaluation of one sample, and evaluate the next sample to evaluate the sensory elements on a 5-point scale (5 -point box scale). The sensory evaluation staff consisted of 15 trained panelists for taste and flavor evaluation, and they were displayed on a 5-point scale. The evaluation criteria for the above items are as follows, and the results are described in Table 6 below.

<Evaluation criteria>

Degree of drag in the mouth (Gently disappears in the mouth 1 ~ Long lasting in the mouth 5)

Texture strength (softness) (very weak 1 to very strong 5)

Satisfaction with throat swallowing (very dissatisfied 1 to very satisfied 5)

Overall Satisfaction (Very Poor 1 to Very Good 5)

Evaluation items degree of attraction in the mouth Taste intensity (softness) Satisfaction with throat swallowing Overall satisfaction sample 8 4.3 1.0 1.3 2.5 sample 9 4.2 2.5 1.2 2.7 sample 10 4.2 1.25 1.2 3.0 sample 11 4.0 1.5 One 2.5 sample 12 2.8 4.0 3.0 3.3 sample 13 4.0 1.75 1.5 2.5 sample 14 4.3 1.0 1.2 2.5 sample 15 1.7 4.8 4.5 3.8 sample 16 1.8 4.9 4.3 3.6 sample 17 1.8 5.0 4.3 3.4

As shown in Table 6, the sample containing carrageenan and glucomannan showed excellent taste and swallowing satisfaction, which means that cargarinan and glucomannan are important gelling agents in realizing the physical properties of drinking jelly. In particular, the organoleptic properties of samples 15 to 17 including carrageenan, glucomannan, and locust bean gum were the most excellent. In addition, when the weight ratio of carrageenan and glucomannan was 1:1 to 2.5:1, the most excellent function was exhibited. In addition, when the weight ratio of glucomannan and locust bean gum was 4:1 to 10:1, the most excellent sensory function was exhibited.

Example 5. Preparation of experimental animals and consumption of drinking jelly

In the case of using psyllium hull and soluble dietary fiber in combination, the following experiment was conducted to determine the effect of the difference according to the mixing ratio on the effect of defecation and to derive the optimal mixing ratio for the function of improving bowel movements and constipation.

(1) Experimental materials

Loperamide hydrochloride (Loperamide) used in this experiment is in the form of a trans isomer powder, Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA), barium sulfate _{(BaSO 4} ) was obtained from Daejung Chemical Co., Ltd. (gyeonggi-do, Korea), and Arabic gum (Acacia powder) was obtained from Junsei Chemical Co., Ltd. (Tokyo, Japan) was purchased and used.

(2) Experimental animals and breeding conditions

45 5-week-old female Sprague-Dawley rats were purchased from Daehan Biolink (Jincheon, Chungcheongbuk-do, Korea), acclimatized for 1 week, and then used in the experiment. For animal feed, Teklad Global 18% Protein Rodent Diet (2018S, Sterilizable), a general solid feed, was purchased from Envigo Inc (New Jersey, USA). 150 g was measured and supplied. The weight of the rat was measured at a constant time every day to confirm the change in weight. The temperature inside the kennel was 27±1℃, the humidity was 15±5%, and the light-dark cycle was controlled in units of 12 hours (9am-9pm). Animal experiments used in this study were approved by the Animal Experimental Ethics Committee (IACUCs) of Chungnam National University and were performed in compliance with ethical regulations.

(3) Experimental design

After acclimatization of 5-week-old SD female rats for 1 week, loperamide (5 mg/kg of mice) was orally administered twice a day (9 am and 6 pm) for 5 days to induce constipation. After that, samples for each group were orally administered twice a day (9 am and 6 pm) for 4 days. During the whole study period, a certain amount of water and feed were supplied at a certain time every day, and the weight and moisture content of the feces were measured by collecting feces based on 24 hours. After sacrifice, feces in the large intestine were collected and measured.

(4) Treatment of laboratory animals

1) How to induce constipation

Loperamide is an antidiarrheal drug for the treatment of diarrhea. When administered continuously for a certain period of time, mucus secretion and intestinal motility in the large intestine decrease, resulting in a longer transit time. It is a drug that stops diarrhea. It has been known that when loperamide is administered to experimental animals, abdominal distension and loss of appetite appear, leading to a decrease in food intake. In addition, when loperamide is administered continuously, the peristaltic movement of the colon, which repeats contraction and relaxation, is reduced, and the movement speed of the stool is reduced. It is known to appear similar to this. Therefore, in this study, constipation was induced by using loperamide. The body weights of all SD rats were measured every day for 5 days, and the average weight was calculated and constipation was induced by oral administration of 2 ml per rat twice a day at 9 am and 6 pm to 5 mg/kg mice.

2) Intake of psyllium hull and soluble dietary fiber mixture

After inducing constipation, a mixture of NMD as a psyllium hull and soluble dietary fiber of a different formulation for each group was orally administered for 4 days. The average body weight (152.7 g) of SD rats was measured before the start of oral administration, and the appropriate amount of the mixture to be consumed per day was determined using 0.16, a safety factor of rats. After putting the mixture powder in sterile distilled water, it was sufficiently vortexed so that the sample could be well dissolved. The sample was immersed in a 37℃ water bath 1 hour before oral administration, and taken out immediately before administration.

Dissolve the sample of Group 2 containing 4.59 g of psyllium husk, which is expected to have the highest viscosity, in distilled water and measure the highest concentration with the viscosity that can be administered orally. As a result, 15.71 mg mixture/ml dH ₂ O ingestion in a volume of 6 ml. Since the required amount per day can be ingested, it was administered orally twice a day at 9 am and 6 pm in 3 ml each. Table 7 shows the experimental design and mixture information. The number of animals in each experimental group was 5 animals.

division Psyllium hull (g) NMD(g) Total weight (g/day) Loperamide
(5mg/kg rat/day) Group 1
(control group) 0 0 0 + Group 2 4.59 2.94 7.53 + Group 3 2.94 2.94 5.88 + Group 4 1.44 2.94 4.38 + Group 5 0.59 2.94 3.53 + Group 6 0 2.94 2.94 + Group 7 4.59 0 4.59 + Group 8 4.06 0.43 4.49 +

3) Weight change and dietary intake

Changes in body weight of each group during the experimental period are shown in FIG. 2 and Table 8. The average body weight of each group increased normally during the experiment, and the difference in body weight between groups was not statistically significant in one-way ANOVA with Duncan's multiple range test. Therefore, it could be seen that the induction of constipation or intake of each sample did not affect the body weight of each group. Day 1; p=0.162, Day 2; p=0.098, Day 3; p=0.316, Day 4; p=0.38, Day 5; p=0.145, Day 6; p=0.158, Day 7; p=0.092, Day 8; p=0.157, Day 9; p=0.096.

division NS before induction of constipation (p=0.149) NS after induction of constipation (p=0.098) NS after sample intake (p=0.092) Group 1 (control) 134.88±2.10 151.4±3.44 159.14±2.18 Group 2 137±1.56 153.64±3.59 161.3±3.11 Group 3 138.725±3.83 154.2±4.25 161.075±4.30 Group 4 131.7±4.55 152.1±2.70 159.5±3.61 Group 5 131.56±3.00 155.65±3.29 161.16±3.42 Group 6 137.94±1.58 154.88±1.40 161.8±2.18 Group 7 137.38±0.89 153.56±1.66 161.8±1.33 Group 8 138.54±3.72 158.84±3.10 168.32±3.36

(5) Statistical analysis

For statistical processing of experimental results, SPSS/Windows 24.0 (SPSS Inc. Chicago, USA) program was used, and one-way ANOVA was performed on the difference between the mean values of three or more groups. After one-way ANOVA, to check the difference between independent variables, a post hoc comparison was performed using Duncan's multiple range test, and it was determined to be significant when p<0.05. To confirm the difference from the control group, student's t-test was used, and it was determined to be significant when p<0.05.

Example 6. Evaluation of defecation efficacy of drinking jelly using experimental animals (1)

In the defecation activity improvement experiment, when the amount of stool increased, intestinal peristalsis was promoted, and the defecation time decreased, reflecting the improvement in defecation activity. Therefore, in this experiment, in order to investigate the effect of defecation according to the mixing ratio of psyllium hull and soluble dietary fiber (NMD), according to the experimental animal and treatment method of Example 5, after induction of constipation and 24 hours after sample intake, Stools were collected and the weight, number of feces, shape, and moisture content (softness of feces) were measured.

During the total experimental period, feces were collected at a set time (9am) every day from two days before Loperamide administration, and the shape, number and weight of feces were measured for 24 hours. The collected stools were then completely dried in a dry oven at 70°C for 72 hours. g)*100) was measured.

(1) the weight of the side

After induction of constipation in all groups, including the control group, the weight of stool decreased rapidly, confirming that constipation was induced by loperamide. After ingestion of the sample, the amount of feces increased in all groups (FIG. 3). Even in the control group, the fecal weight increased despite the odor. This is because loperamide administration was stopped during sample intake, so the feces that stayed in the intestine due to constipation were discharged, which is thought to be the natural recovery of the experimental animals.

For comparison between the experimental groups, the amount of stool increase due to the cessation of constipation induction of the control group was set to 100%, and the increase in stool weight by the sample for each group was calculated in % and shown in FIG. 4 . As shown in FIG. 4 , Group 4 showed the highest amount of stool increase by the sample, followed by Group 2 and Group 3 with the highest amount of stool increase by the sample. When compared with the NMD alone group (Group 6) and the chamfered skin alone group (Group 7), it was found that the rate of change of change was higher in Groups 2, 3, and 4. Overall, it could be seen that the rate of increase in the change weight among the psyllium hull mixed groups was not dependent on the amount of psyllium hull.

The ranking of the increase in stool weight in each experimental group compared to the fasting group was as follows:

Group 4 (183.2%)> Group 2 (176.0%)> Group 3 (167.9%)> Group 5 (159.5%)> Group 1 (100%)

In the control groups, the psyllium hull alone group (Group 7) showed more defecation effect than the NMD alone group (Group 6), and it can be seen that the group 8 showed a significantly smaller increase in stool weight compared to the psyllium hull mixed group.

The ranking of the increase in stool weight of the entire test group including the control group compared to the first group was as follows:

Group 4 (183.2%)> Group 2 (176.0%)> Group 3 (167.9%)> Group 7 (165.8%)> Group 5 (159.5%)> Group 6 (150%)> Group 8 (130.5%)> Group 1 (100%)

(2) Number of sides

5 is a view showing the number of sides by group. When constipation was induced, the number of stools also decreased and showed a pattern that increased again after sample intake. Except for Group 8, the number of stools in all experimental groups increased significantly, and in Group 2, Group 4, and Group 5, the number of stools increased significantly after ingestion of the sample.

(3) the shape of the side

6 is a photograph taken by collecting the total feces excreted for 24 hours, and the size and shape of the feces after ingestion of the sample can be seen. After ingestion of the sample, the size of the feces in each group increased, and Group 4 had the largest feces. In the case of Group 3, Group 4, and Group 5, it was determined that the weight of the sides increased as the size of the sides increased rather than the number of sides. The blue scale bar in FIG. 6 represents 1 cm.

(4) water content in feces

According to the experiment to improve defecation activity, when the water content of the stool was increased, not only the volume of the stool increased, but also the peristalsis of the intestine was promoted and the defecation time was decreased, which indicates that the defecation activity was improved. Therefore, in this experiment, the water content of feces was checked to determine the effect of defecation according to the mixing ratio of dietary fiber NMD and psyllium containing dietary fiber.

The feces collected for 24 hours were ^{dried at 70 o} C for 72 hours, and then the weight was measured to calculate the moisture content of the feces. As shown in FIG. 7 , there was no change in the water content after induction of constipation and after ingestion of the sample in the control group, but the water content increased significantly in the experimental group.

In FIG. 8, the water content increased after ingestion of the sample was calculated as % based on the post-constipation induction in each group. Among the experimental groups, Group 4 showed the highest water content increase after sample intake, followed by Group 5 and Group 2 with the next highest water content increase. Group 4 showed a higher water content than NMD alone (Group 6) or psyllium hull alone (Group 7).

The ranking of the fecal moisture content in each experimental group compared to the succulent group was as follows:

Group 4 (48.3%)> Group 5 (43.8%)> Group 2 (29.5%) > Group 3 (22.6%)> Group 1 (-3.5%)

Table 9 summarizes the weight, number, and water content of feces for 24 hours after ingestion of the sample. As shown in Table 9, it was found that Group 4 had the highest rate of increase in weight, size, and moisture content of excreted feces within 24 hours after sample intake.

ranking Variation weight increase rate Variable increase rate rate of increase in stool size Moisture content increase rate One Group 4 (183.2%) Group 2 Group 4 Group 4 (48.3%) 2 Group 2 (176.0%) Group 5 Group 3 Group 5 (43.8%) 3 Group 3 (167.9%) Group 4 Group 5 Group 2 (29.5%) 4 Group 5 (159.5%) Group 3 Group 2 Group 3 (22.6%) 5 Group 1 (100%) Group 1 Group 1 Group 1 (-3.5%)

Example 7. Evaluation of defecation efficacy of drinking jelly using experimental animals (2)

(5) Measurement of the weight of feces in the large intestine

The weight of the feces remaining in the large intestine of the sacrificed experimental animals was measured to determine the feeling of residual stool when the psyllium hull and soluble dietary fiber mixture was ingested. The factor to be considered as the effect of improving bowel movements in this study is the ability to move the colon muscles (peristalsis). Based on the above data, it is thought that the intake of psyllium hull dietary fiber increases the water content of the stool, which increases the volume of the stool, and induces peristaltic movement of the colon muscle, thereby improving defecation activity. However, since the feces remaining in the large intestine induce a feeling of residual stool, in this experiment, the weight of the feces remaining in the large intestine was observed after sacrificing the experimental animals.

The standard error was large due to the difference between the experimental animals in the weight of the feces remaining in the large intestine, compared to the Groups with statistically significant differences in stool weight were Group 3**(p=0.0019), Group 4**(p=0.002), Group 5*(p=0.039), Group 6*(p=0.037) , was Group 7* (p=0.025) (Fig. 9, *p<0.05, **p<0.01). In particular, group 3 and group 4 showed the largest significant difference in all groups including the control group.

Example 8. Evaluation of defecation efficacy of drinking jelly using experimental animals (3)

(6) colon histopathological analysis

1) Evaluation of the anatomical structure and safety of the large intestine

In this study, loperamide was used for 5 days to induce constipation. Therefore, in order to find out whether 1) Loperamide itself is toxic, and 2) Psyllium shell mixture itself in different weight ratios caused damage to the colon structure, the colon tissue was fixed using 10% formalin and then H&E stained. was confirmed. Colonic tissues were fixed and stained with Haematoxylin and eosin stain (H&E) to check for toxicity and inflammation caused by induction of constipation by loperamide and ingestion of psyllium hull mixture.

In general, the structure of the large intestine consists of villi, and when there is damage to the colon tissue, immune cells invade the lumen or the length of the villi is shortened. In case of severe damage, the villi structure itself is destroyed, making it impossible to absorb nutrients, and pathogens including E. coli enter the intestinal system and cause inflammation. Therefore, colonic tissue was confirmed through H&E staining. In this experiment, in all groups including the control group, there was no destruction of the colon mucosa or reduction in the length of the villi, and the anatomical structure of the colon was normal in all groups ( FIG. 10 ).

Based on the nucleus of the cells stained with purple dots, in a normal state, colonic epithelial cells are arranged in a line along a short villi structure. However, when inflammation is present in the large intestine, immune cells including monocytes invade the mucous membrane irregularly, and as the inflammation worsens, the immune cells spread irregularly from the tip of the villi to the inner muscle layer. This is called monocyte infiltration. In this experiment, Group 1 (no intake group) showed the relatively severe inflammation pattern. In the control group, immune cells pass through the epithelium and invade to the inside of the connective tissue called the lamina propria (a part of the mucosa under the villi and the thin layer of connective tissue) to form a group or it can be confirmed that they are infiltration throughout the villi (Fig. arrow in 10).

In Group 2, monocyte infiltration was found only at the tip of the villi. In Group 3 and Group 4, a relatively small amount of monocyte infiltration was found between the tip of the villi and the villi compared to that of the first group. Therefore, it can be seen that the degree of inflammation was relatively lower than that of the saengchwi group. Therefore, it was found that the dietary fiber intake for 3 days had an effect on the recovery of the mild inflammatory response in the control group.

Among the control groups, a large number of goblet cells specifically secreting mucus were found in Group 6, monocyte infiltration was found only at the tip of the villi in Group 7, and monocyte infiltration was found up to the lamina propria in Group 8.

2) Inflammatory state of the large intestine

In order to confirm the inflammatory state of the colon tissue, mRNA expression of IL6 (fw: ACA GCG ATG ATG CAC TGT CAG, rv: ATG GTC TTG GTC CTT AGC CAC) was measured in the colon tissue. Briefly, colon tissue was put in Tri-reagent (MRC Inc., USA) and homogenized using MACS™ Disociator (Milteni Biotec Co, Bergisch Gladbach, Germany), then RNA was made and cDNA was synthesized, followed by 2X Taq Basic PCR Master RT-PCR was performed using Mix (BioFact, Korea). After electrophoresis on 3% agarose gel, bands were observed under UV light using E-Graph (AE-9000 E-Graph, ATTO, Japan). HRPT (fw: GCT GAC CTG CTG GAT TAC AT, rv: CCC GTT GAC TGG TCA TTA CA) was used as a loading control to measure the total amount of cDNA.

As seen in the H&E staining of FIG. 10, when constipation was induced, inflammation occurred in the colon. Therefore, the mRNA expression level of interleukin 6 (IL-6), known as the most common inflammatory marker, was confirmed in colon tissue ( FIG. 11b ). HRPT was used as a loading control to check whether the total cDNA amount was the same. After measuring and quantifying the intensity of the band of RT-PCR with differences between individuals through the Image J program, statistical significance was analyzed (FIG. 11b B). The expression of IL-6 was the highest in the first group (group 1), and although there was a difference between subjects when compared with the control group, the expression of IL-6 was significantly inhibited in Group 2 and Group 4 the most.

The order of IL-6 expression level by experimental group compared to the fast-drinking group is as follows.

Group 1> Group 5 > Group 3 > Group 2 * > Group 4 *

In the control group, it can be seen that the expression of IL-6 was significantly suppressed in group 7, and there was a relatively individual difference between group 8 and the expression of IL-6 was not significantly suppressed.

3) secretion of mucus in the large intestine

In order to examine whether the psyllium hull mixture affects the secretion of mucus in the colon and improves bowel activity, the production and secretion of mucin, a component of intestinal mucus, was evaluated for acidity with Periodic acid-Schiff (PAS: neutral mucin stain) staining and Alcian Blue (AB). : Acidic mucin stain) was confirmed. Alcian Blue (Acidic mucin) and PAS (Neutral mucin) staining was requested to T&P Bio (Gyeonggi-do, Korea) to check the change in the amount of mucin secretion in the colon and the acidity of the mucus due to ingestion of the psyllium hull mixture.

The secretion of mucus in the large intestine is used as one of the indicators for improving bowel movements, and the more mucus secretion in the large intestine, the smoother the bowel movement. Therefore, in this study, the secretion amount, secretion pattern, and acidity of mucus were confirmed by PAS staining and AB staining.

PAS staining shows the location and amount of acidic mucus in pink, and AB staining shows the location and amount of neutral mucus in light blue (FIG. 11a). It appears that the secreted neutral mucus forms a mucus layer and is located in the lumen.

In Group 1 (no intake group), the synthesis and secretion of mucus was low. Overall, Group 3 secreted the most mucus, followed by Group 4, Group 2, and Group 5, which produced and secreted the most mucus. When the environment in the large intestine is acidic, harmful bacteria among pathogenic bacteria and Escherichia coli are difficult to survive, and when the intestinal pH is slightly acidic, it is a healthy intestinal environment where beneficial bacteria can thrive. Therefore, in the case of Group 3 and Group 4, which induce the secretion of acidic mucus, it can be expected not only to improve bowel activity in the short term, but also to improve intestinal health in the long term. In addition, in Group 3, Group 4, and Group 5, not only the synthesis amount but also the secretion amount of neutral mucus increased.

Example 9. Evaluation of defecation efficacy of drinking jelly using experimental animals (4)

Sample preparation

Table 10 shows the products and experimental designs used in this study. In Table 10, vulgaris, dulcofiber, and araxyl were purchased and used commercially, and sample 15 prepared in Example 2 was used as the drinking jelly according to an embodiment of the present invention.

Group manufacturer human dose/day animal dose/day formulation n Control
(No intake group) - - - - 5 vulgaris Namyang 150ml 2.640ml liquid 5 Dulco Fiber Sanofi 21.2g 373mg Gumi 5 Araxyl Bukwang Pharmaceutical 4g 70mg granules 5 drinking jelly Samyang Corporation 120g 2.112g drinking jelly 8

Based on the average weight of rats, which is 169 g, on the 3rd day after induction of constipation, the appropriate dose of each product was set according to the same volume of 2 ml. After the induction of constipation, the sample was orally administered once a day at 9 am on the 4th day. Immediately before oral administration, an appropriate amount of the product was diluted in sterile distilled water, thoroughly mixed so that the sample could be well dissolved, and left at room temperature for 30 minutes- Homogenize for 1 hour. Bulgaris and Drinking Jelly were orally administered in an appropriate amount as they were. Dulco fiber was frozen at -80°C and then converted into powder using a mixer, and an appropriate amount was measured once a day, diluted in 2ml of distilled water, and then homogenized at room temperature for 30 minutes to 1 hour. Araxyl was converted into powder using a stick, and an appropriate amount was measured once a day, diluted in 2 ml of distilled water, and homogenized at room temperature for 30 minutes to 1 hour.

Experimental results and considerations

1. Weight change

Table 11 shows the change in body weight of each group during the experimental period. The average body weight of each group increased normally during the experiment, and the difference in body weight between the groups was not statistically significant every day. As a result, induction of constipation or intake of each product did not affect the body weight of each group. Day 0; p=0.310, Day 1; p=0.071, Day 2; p=0.076, Day 3; p=0.138, Day 4; p = 0.183.

Body weight (g) NS before constipation induction (p=0.310) NS after induction of constipation (p=0.076) NS after sample intake (p=0.183) Control (no intake group) 150.76±5.74 154.18±5.68 165.1±6.95 vulgaris 151.44±1.37 152.34±0.81 166.2±1.77 Dulco Fiber 156.84±4.40 162.86±3.38 173.4±4.07 Araxyl 155.5±5.48 159.36±4.32 169.5±4.12 drinking jelly 154.89±9.37 159.64±10.89 174±11.82

Values are Mean±S.E., NS: No Significant difference between the groups by one-way ANOVA with Duncan’s multiple range test

2. Fecal weight, number, shape, and moisture content excreted for 24 hours after sample intake (stool softening)

In order to confirm the defecation effect of each product, the weight, number, and moisture content of the stool were checked during the experiment period. In the defecation activity improvement experiment, when the amount of stool increased, intestinal peristalsis was promoted, and the defecation time decreased, reflecting the improvement in defecation activity. Therefore, in this experiment, the wet weight and the number of stools were measured by collecting the feces of the experimental animals by dividing into before constipation induction, after constipation induction, and after sample intake. was calculated and shown.

2.1 Change in side weight

After induction of constipation by loperamide, the weight of the feces in all groups, including the control group, decreased rapidly, confirming that the induction of constipation was successful in the experimental animal model. The control group showed an increase in the weight of the feces after the induction of constipation was finished despite the odor of the sample, but there was no significant difference (FIG. 12a). This is considered to be the natural recovery of the experimental animals, in which the feces that stayed in the intestine were discharged by inducing constipation because the administration of loperamide was stopped on Day 4, the day of product intake. Considering the natural recovery, the weight of excreted feces after ingestion of the sample was statistically significantly increased in all groups except for araxyl when compared with the control group at the time point after ingestion of the sample (FIG. 12a).

For a relative comparison between the experimental groups, the amount of stool increase by stopping the induction of constipation in the control group was set as 100%, and then the amount of increase in stool weight due to product intake for each group was calculated in % (FIG. 12b). In the drinking jelly group, the weight of feces showed the largest statistically significant increase rate than before induction of constipation. The amount of stool increase in the Araxyl, Vulgaris, and Dulcofiber groups was not significantly increased compared to that of the Susukchwi group. When compared with after induction of constipation, the ranking according to the amount of stool weight increase by product was as follows (*p<0.05, **p<0.01):

Drinking jelly (180.33%)* > Araxyl (147.87%)> Vulgaris (136.83%)> Dulcofiber (124.15%)> Control (100%)

2.2 Change in side size

13 is a picture of the feces excreted for 24 hours after induction of constipation and after ingestion of the sample, and the area (size) of each feces was calculated through the Image J program. After measuring the area of feces for each group, the area of one excreted feces per animal was measured by dividing by the number of feces.

Compared with the control group, the size of the drinking jelly group increased statistically significantly, and in particular, the area of the feces in the group that ate the drinking jelly increased significantly more than 3 times in the increase rate of the average fecal area. The control group, vulgaris, araxyl and dulcofiber did not show statistical significance. After induction of constipation, when the increase in the size of the feces of the Shuchuchi group was set as 100%, the ranking according to the fecal size increase was as follows (**p<0.01):

Drinking jelly (336%)**> Vulgaris (273%)> Dulcofiber (192%)> Araxyl (182%)> Control (100%)

2.3 Changes in the water content of stool

In the defecation activity improvement experiment, it is known that an increase in the water content of the stool results in an increase in stool volume, promotion of intestinal peristalsis, and a decrease in excretion time, which reflects the improvement in defecation activity. In this study, the feces collected for 24 hours were dried at 70°C for 72 hours to measure the dry weight, and then the moisture content of the feces was calculated and converted into %. There was no change in the water content before induction of constipation and after ingestion of the sample in the sabchuchwi group, but in the experimental group, the water content was significantly increased in all groups except Araxyl (FIG. 14).

As a result of converting the increase in water content by product intake of each group into %, the water content was significantly higher in the order of drinking jelly, dolco fiber, vulgaris, araxyl, and control group. In the drinking jelly group, the p-value was less than 0.001, so it was confirmed that the p-value was higher than that of Dolco Fiber or Vulgaris, which had a p-value of less than 0.05. Compared to after induction of constipation, the ranking according to the increase in fecal moisture is as follows (*p<0.05, ***p<0.001):

Drinking jelly (15%)***> Dulcofiber (11%)*> Vulgaris (7%)*> Araxyl (4%)> Control (1%)

Table 12 summarizes the results for stool weight, number of stools, stool size, and moisture content. In the case of the drinking jelly group, the increase in stool weight, stool size, and water content were the best. It was inferred that drinking jelly increases the weight and size of the stool by increasing the water content of the stool. Therefore, considering the overall increase in the weight/size/moisture content of feces, it was judged that drinking jelly was the most effective product in all product groups.

ranking stool increase rate rate of increase in stool size Moisture content increase rate One Drinking Jelly* Drinking Jelly** Drinking Jelly*** 2 Araxyl vulgaris Dulco Fiber* 3 vulgaris Dulco Fiber Vulgaris* 4 Dulco Fiber Araxyl Araxyl 5 control control control

Significant difference between before and after product treatment was tested by t-test. *p<0.05, **p<0.01, ***p<0.001

3. Weight of residual stool in the large intestine

In this study, in order to verify the immediate effect of defecation improvement, 24 hours after ingestion of the product without fasting, the experimental animals were sacrificed and the weight of feces in the large intestine was observed.

A photograph of the cecum and large intestine is shown in FIG. 15A. The weight of feces remaining in the large intestine was decreased in all experimental groups compared to the control group (FIG. 15b). However, only drinking jelly showed a statistically significant decrease (p<0.5) compared to the control group. In the case of the vulgaris group, there was a large difference in the number of feces per individual and the resulting feces weight, so the average feces weight decreased, but no statistical significance was shown due to the high inter-individual error. The ranking according to the weight of the stool remaining in the large intestine is as follows (*p<0.05).

Control > Araxyl > Dulcofiber > Vulgaris > Drinking Jelly*

Therefore, the weight of the feces remaining in the large intestine showing a feeling of residual stool is considered to have the best effect as a bowel movement improvement product because there is significantly less drinking jelly.

4. Structure and mucus secretion in the large intestine and cecum

The purpose of this study is to measure the bowel movement improvement effect of drinking jelly containing psyllium hull compared to other products.

Since Loperamide was used for 3 days to induce constipation, 1) Loperamide itself was toxic, and 2) the product itself caused damage to the colon structure. The anatomical structure was confirmed.

Increased intestinal mucus secretion facilitates bowel movements. Therefore, in this experiment, the production and secretion of mucin, a component of intestinal mucus, was analyzed with Periodic acid-Schiff (PAS: neutral mucin stain) staining and Alcian Blue (AB: acidic mucin stain) to check the mucin secretion and acidity of the colon and cecum. ) was confirmed.

4.1 Evaluation of the anatomical structure and stability of the colon and appendix

In case of damage to the colon tissue, immune cells invade into the special structure of the large intestine, the villi, or the length of the villi is shortened. Pathogens enter the intestinal system and cause inflammation. Another study reported that eating an excess of insoluble dietary fiber without adequate fluid intake may cause the stool to become rather hard.

First, the structure of the colon and cecum and the degree of infiltration of immune cells were measured by H&E staining (FIG. 16). In this experiment, there was no destruction of colonic mucosa or reduction of the length of the villi in all groups including the control group, and the anatomical structures of the colon and cecum appeared to be normal in all groups. This means that ingestion of loperamide or the product did not adversely affect the anatomy of the colon and cecum.

The part stained with purple dots is the nucleus of the cells, and in a normal state, it can be confirmed by the purple dots that the epithelial cells of the colon are arranged in a line along the short villi structure. However, when there is inflammation in the large intestine, immune cells including monocytes invade the mucosa, which can be confirmed by abnormal and irregular purple dots (monocyte infiltration). The more severe the inflammation, the more immune cells invade from the tip of the villi to the inner muscle layer. In this experiment, the group showing the most severe inflammatory pattern was the suppository group. Immune cell infiltration was seen in the lamina propria through the epithelium in the case of the supreme group, but infiltration between the villi was overall in the other groups (indicated by arrows in FIG. 16). Relatively, the degree and number of infiltration were less in the drinking jelly group than in vulgaris, araxyl, and dulcofiber. In the case of dulcofiber, it can be confirmed that local infiltration into the lamina propria formed a cluster. However, in the case of drinking jelly, there were few immune cells that invaded the lamina propria, and the number of places where immune cells were gathered was also small.

4.2 Mucous secretion of the large intestine and cecum

PAS staining shows the location and amount of acidic mucus in pink, and AB staining shows the location and amount of neutral mucus in light blue (FIG. 17). In both acidic and neutral mucus, the synthesis and secretion of mucus in the colon and cecum were the lowest in the control group.

In the case of the drinking jelly group, it is thought that the amount of synthesis and secretion of acidic mucus in the large intestine and cecum continued to be relatively similar. In the case of dulcofiber, the colon and caecum showed less acidic mucus synthesis and secretion similar to that of the control group, and in the case of araxyl, the large intestine and cecum showed a pattern of secretion more than the synthetic amount. In the case of vulgaris, the large intestine showed a higher secretion than the synthetic amount, and the cecum showed less mucus synthesis and secretion similar to that of the control group.

In the large intestine and caecum, the control group showed less synthesis and secretion of neutral mucus. Relatively, it seems that drinking jelly has a lot of synthesis of neutral mucus in the large intestine and caecum. In the cecum, all groups except drinking jelly showed a similar level of neutral mucus to the control group.

Therefore, it was found that the drinking jelly according to the present invention promotes the active production and secretion of both acidic and neutral mucus in the large intestine and caecum.

Claims (18)

Psyllium hulls, a gelling agent, and water;
The gelling agent includes carrageenan and glucomannan,
The carrageenan and the glucomannan are included in a weight ratio of 0.5:1 to 4:1,
As a gelling agent, that has spreadability of 90% or less compared to a control that does not contain carrageenan,
Drinking jelly composition. The composition according to claim 1, which has one or more properties selected from the group consisting of the following (1) to (4):
(1) hardness of 100 to 1,500 (g),
(2) a viscosity of greater than 1,800 to less than 7,300 (mPa.s);
(3) pH is 2 to 5,
(4) Yield is 5 to 50 (%). The composition according to claim 1, wherein the moisture content is at least 50% by weight. According to claim 1, Based on 100% by weight of the composition, the psyllium hull is included in 0.1 to 10% by weight, the composition. According to claim 1, Based on 100% by weight of the composition, the gelling agent will be included in 0.01 to 5% by weight, the composition. The method according to claim 1, wherein the gelling agent is selected from the group consisting of locust bean gum, xanthan gum, guar gum, agar, pectin, gelatin, gum arabic, gellan gum, alginic acid, konjac, cellulose, tara gum, and tamarind gum. wherein the composition further comprises at least one species. The composition of claim 1, wherein the gelling agent further comprises locust bean gum. The composition of claim 7, wherein the glucomannan and the locust bean gum are included in a weight ratio of 1:1 to 15:1. The composition of claim 7, wherein the carrageenan, the glucomannan, and the locust bean gum are included in a weight ratio of 9 to 10: 6 to 8: 1. delete The composition of claim 1, wherein the composition further comprises one or more selected from the group consisting of a gelation accelerator, sugars, high-sweetness sweeteners, vitamins, organic acids, acidity regulators, fruit concentrates, minerals, and fragrances. The composition of claim 1, wherein the composition further comprises indigestible maltodextrin. The composition of claim 12, wherein the indigestible maltodextrin is included in an amount of 0.05 to 10% by weight, based on 100% by weight of the composition. The composition of claim 12, wherein the psyllium hull and the indigestible maltodextrin are included in a weight ratio of 1: 0.5 to 1:5. According to claim 1, wherein the composition is a composition for improving constipation, the composition. The composition of claim 1, wherein the composition is a composition for improving reduced bowel movements and/or reducing residual stool in the large intestine. The composition of claim 1, wherein the composition induces at least one characteristic selected from the group consisting of the following (1) to (9):
(1) increase the amount of stool,
(2) increase in stool size;
(3) increase in the water content of feces,
(4) reduction of residual stool weight in the large intestine;
(5) improvement of colon inflammation;
(6) improvement of villous monocyte infiltration;
(7) promote the secretion of mucus in the large intestine,
(8) promote the secretion of acidic mucus in the large intestine;
(9) promote the secretion of neutral mucus in the large intestine. 18. The composition according to any one of claims 1 to 9 and 11 to 17, wherein the composition is a pouch-type jelly, a cup-type jelly, or a stick-type jelly.

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